LOCUS       DADOOS010000001        82692 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.001, whole genome shotgun sequence.
ACCESSION   DADOOS010000001 DADOOS010000000
VERSION     DADOOS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 82692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.001"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            17..535
                     /locus_tag="J8C37_000001"
     CDS             17..535
                     /locus_tag="J8C37_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ5303472.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="J8C37_000002"
     CDS             663..1346
                     /locus_tag="J8C37_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAZ5303473.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="J8C37_000003"
     tRNA            1655..1728
                     /locus_tag="J8C37_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="J8C37_000004"
     CDS             1887..2246
                     /locus_tag="J8C37_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAZ5303474.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="J8C37_000005"
     CDS             2369..4000
                     /locus_tag="J8C37_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAZ5303475.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="J8C37_000006"
     CDS             complement(4062..4703)
                     /locus_tag="J8C37_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAZ5303476.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="J8C37_000007"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="J8C37_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAZ5303477.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="J8C37_000008"
     CDS             5320..5664
                     /locus_tag="J8C37_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAZ5303478.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="J8C37_000009"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="J8C37_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAZ5303479.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6377..9211
                     /locus_tag="J8C37_000010"
     CDS             6377..9211
                     /locus_tag="J8C37_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5303480.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="J8C37_000011"
     CDS             9402..9893
                     /locus_tag="J8C37_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAZ5303481.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /gene="manX"
                     /locus_tag="J8C37_000012"
     CDS             9996..10985
                     /gene="manX"
                     /locus_tag="J8C37_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAZ5303482.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="J8C37_000013"
     CDS             11018..11821
                     /locus_tag="J8C37_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAZ5303483.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="J8C37_000014"
     CDS             11842..12756
                     /locus_tag="J8C37_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAZ5303484.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="J8C37_000015"
     CDS             12839..13213
                     /locus_tag="J8C37_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAZ5303485.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="J8C37_000016"
     CDS             13352..13597
                     /locus_tag="J8C37_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ5303486.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13769..14833
                     /locus_tag="J8C37_000017"
     CDS             13769..14833
                     /locus_tag="J8C37_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAZ5303487.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15079..15504
                     /locus_tag="J8C37_000018"
     CDS             15079..15504
                     /locus_tag="J8C37_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ5303488.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15497..15946
                     /gene="nusB"
                     /locus_tag="J8C37_000019"
     CDS             15497..15946
                     /gene="nusB"
                     /locus_tag="J8C37_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAZ5303489.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16043..16897
                     /locus_tag="J8C37_000020"
     CDS             16043..16897
                     /locus_tag="J8C37_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAZ5303490.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16898..18235
                     /locus_tag="J8C37_000021"
     CDS             16898..18235
                     /locus_tag="J8C37_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAZ5303491.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18240..18464
                     /locus_tag="J8C37_000022"
     CDS             18240..18464
                     /locus_tag="J8C37_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAZ5303492.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18466..19356
                     /locus_tag="J8C37_000023"
     CDS             18466..19356
                     /locus_tag="J8C37_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAZ5303493.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19365..20183
                     /locus_tag="J8C37_000024"
     CDS             19365..20183
                     /locus_tag="J8C37_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAZ5303494.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20290..20739
                     /locus_tag="J8C37_000025"
     CDS             20290..20739
                     /locus_tag="J8C37_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAZ5303495.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20757..22439
                     /gene="recN"
                     /locus_tag="J8C37_000026"
     CDS             20757..22439
                     /gene="recN"
                     /locus_tag="J8C37_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAZ5303496.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22494..22610)
                     /locus_tag="J8C37_000027"
     CDS             complement(22494..22610)
                     /locus_tag="J8C37_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAZ5303497.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22683..23639)
                     /locus_tag="J8C37_000028"
     CDS             complement(22683..23639)
                     /locus_tag="J8C37_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAZ5303498.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23810..24178
                     /locus_tag="J8C37_000029"
     CDS             23810..24178
                     /locus_tag="J8C37_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAZ5303499.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24348..24779
                     /gene="mraZ"
                     /locus_tag="J8C37_000030"
     CDS             24348..24779
                     /gene="mraZ"
                     /locus_tag="J8C37_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAZ5303500.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24800..25759
                     /gene="rsmH"
                     /locus_tag="J8C37_000031"
     CDS             24800..25759
                     /gene="rsmH"
                     /locus_tag="J8C37_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAZ5303501.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25764..26165
                     /gene="ftsL"
                     /locus_tag="J8C37_000032"
     CDS             25764..26165
                     /gene="ftsL"
                     /locus_tag="J8C37_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAZ5303502.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26166..28358
                     /locus_tag="J8C37_000033"
     CDS             26166..28358
                     /locus_tag="J8C37_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAZ5303503.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28384..29346
                     /locus_tag="J8C37_000034"
     CDS             28384..29346
                     /locus_tag="J8C37_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAZ5303504.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29360..30721
                     /locus_tag="J8C37_000035"
     CDS             29360..30721
                     /locus_tag="J8C37_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAZ5303505.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30747..31835
                     /gene="murG"
                     /locus_tag="J8C37_000036"
     CDS             30747..31835
                     /gene="murG"
                     /locus_tag="J8C37_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAZ5303506.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31850..33070
                     /locus_tag="J8C37_000037"
     CDS             31850..33070
                     /locus_tag="J8C37_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAZ5303507.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33226..34551
                     /gene="ftsA"
                     /locus_tag="J8C37_000038"
     CDS             33226..34551
                     /gene="ftsA"
                     /locus_tag="J8C37_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAZ5303508.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34574..35815
                     /gene="ftsZ"
                     /locus_tag="J8C37_000039"
     CDS             34574..35815
                     /gene="ftsZ"
                     /locus_tag="J8C37_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAZ5303509.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35832..36509
                     /locus_tag="J8C37_000040"
     CDS             35832..36509
                     /locus_tag="J8C37_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAZ5303510.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36523..37122
                     /locus_tag="J8C37_000041"
     CDS             36523..37122
                     /locus_tag="J8C37_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAZ5303511.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37137..37424
                     /locus_tag="J8C37_000042"
     CDS             37137..37424
                     /locus_tag="J8C37_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAZ5303512.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37473..38255
                     /locus_tag="J8C37_000043"
     CDS             37473..38255
                     /locus_tag="J8C37_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAZ5303513.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38276..38983
                     /locus_tag="J8C37_000044"
     CDS             38276..38983
                     /locus_tag="J8C37_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAZ5303514.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39245..42043
                     /gene="ileS"
                     /locus_tag="J8C37_000045"
     CDS             39245..42043
                     /gene="ileS"
                     /locus_tag="J8C37_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAZ5303515.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42092..43618)
                     /locus_tag="J8C37_000046"
     CDS             complement(42092..43618)
                     /locus_tag="J8C37_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAZ5303516.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43633..44280)
                     /locus_tag="J8C37_000047"
     CDS             complement(43633..44280)
                     /locus_tag="J8C37_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAZ5303517.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44464..44793)
                     /locus_tag="J8C37_000048"
     CDS             complement(44464..44793)
                     /locus_tag="J8C37_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAZ5303518.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44970..45698
                     /gene="sfsA"
                     /locus_tag="J8C37_000049"
     CDS             44970..45698
                     /gene="sfsA"
                     /locus_tag="J8C37_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAZ5303519.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45714..46727
                     /locus_tag="J8C37_000050"
     CDS             45714..46727
                     /locus_tag="J8C37_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ5303520.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46727..48004
                     /locus_tag="J8C37_000051"
     CDS             46727..48004
                     /locus_tag="J8C37_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAZ5303521.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48067..50769
                     /locus_tag="J8C37_000052"
     CDS             48067..50769
                     /locus_tag="J8C37_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5303522.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50921..51238
                     /locus_tag="J8C37_000053"
     CDS             50921..51238
                     /locus_tag="J8C37_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5303523.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51268..51588
                     /locus_tag="J8C37_000054"
     CDS             51268..51588
                     /locus_tag="J8C37_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ5303524.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51696..53156
                     /locus_tag="J8C37_000055"
     CDS             51696..53156
                     /locus_tag="J8C37_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5303525.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53224..53445
                     /locus_tag="J8C37_000056"
     CDS             53224..53445
                     /locus_tag="J8C37_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303526.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53476..53658
                     /locus_tag="J8C37_000057"
     CDS             53476..53658
                     /locus_tag="J8C37_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAZ5303527.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53658..54071
                     /locus_tag="J8C37_000058"
     CDS             53658..54071
                     /locus_tag="J8C37_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAZ5303528.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54194..55375
                     /locus_tag="J8C37_000059"
     CDS             54194..55375
                     /locus_tag="J8C37_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5303529.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55764..55837
                     /locus_tag="J8C37_000060"
     tRNA            55764..55837
                     /locus_tag="J8C37_000060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55798..55800,aa:Arg,seq:cct)
     gene            complement(55874..56074)
                     /locus_tag="J8C37_000061"
     CDS             complement(55874..56074)
                     /locus_tag="J8C37_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ5303530.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56379..57611)
                     /locus_tag="J8C37_000062"
     CDS             complement(56379..57611)
                     /locus_tag="J8C37_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAZ5303531.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57865..58434
                     /locus_tag="J8C37_000063"
     CDS             57865..58434
                     /locus_tag="J8C37_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5303532.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58612..59052)
                     /locus_tag="J8C37_000064"
     CDS             complement(58612..59052)
                     /locus_tag="J8C37_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAZ5303533.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59210..59974
                     /gene="map"
                     /locus_tag="J8C37_000065"
     CDS             59210..59974
                     /gene="map"
                     /locus_tag="J8C37_000065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAZ5303534.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60006..60929
                     /locus_tag="J8C37_000066"
     CDS             60006..60929
                     /locus_tag="J8C37_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAZ5303535.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61005..62144
                     /locus_tag="J8C37_000067"
     CDS             61005..62144
                     /locus_tag="J8C37_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAZ5303536.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62137..62937
                     /locus_tag="J8C37_000068"
     CDS             62137..62937
                     /locus_tag="J8C37_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ5303537.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62937..63764
                     /locus_tag="J8C37_000069"
     CDS             62937..63764
                     /locus_tag="J8C37_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ5303538.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63742..64476
                     /locus_tag="J8C37_000070"
     CDS             63742..64476
                     /locus_tag="J8C37_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ5303539.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64576..65442
                     /gene="rfbA"
                     /locus_tag="J8C37_000071"
     CDS             64576..65442
                     /gene="rfbA"
                     /locus_tag="J8C37_000071"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAZ5303540.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65456..66028
                     /gene="rfbC"
                     /locus_tag="J8C37_000072"
     CDS             65456..66028
                     /gene="rfbC"
                     /locus_tag="J8C37_000072"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAZ5303541.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66050..67078
                     /gene="rfbB"
                     /locus_tag="J8C37_000073"
     CDS             66050..67078
                     /gene="rfbB"
                     /locus_tag="J8C37_000073"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAZ5303542.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67176..68027
                     /gene="rfbD"
                     /locus_tag="J8C37_000074"
     CDS             67176..68027
                     /gene="rfbD"
                     /locus_tag="J8C37_000074"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAZ5303543.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68062..70095
                     /locus_tag="J8C37_000075"
     CDS             68062..70095
                     /locus_tag="J8C37_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303544.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70139..71419
                     /locus_tag="J8C37_000076"
     CDS             70139..71419
                     /locus_tag="J8C37_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAZ5303545.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71629..72435
                     /locus_tag="J8C37_000077"
     CDS             71629..72435
                     /locus_tag="J8C37_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303546.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72447..73667
                     /locus_tag="J8C37_000078"
     CDS             72447..73667
                     /locus_tag="J8C37_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303547.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73657..75243
                     /locus_tag="J8C37_000079"
     CDS             73657..75243
                     /locus_tag="J8C37_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ5303548.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75282..77420
                     /locus_tag="J8C37_000080"
     CDS             75282..77420
                     /locus_tag="J8C37_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ5303549.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(77788..78771)
                     /locus_tag="J8C37_000081"
     CDS             complement(77788..78771)
                     /locus_tag="J8C37_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAZ5303550.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79149..80540
                     /locus_tag="J8C37_000082"
     CDS             79149..80540
                     /locus_tag="J8C37_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAZ5303551.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            80556..81596
                     /locus_tag="J8C37_000083"
     CDS             80556..81596
                     /locus_tag="J8C37_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ5303552.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            81615..>82692
                     /locus_tag="J8C37_000084"
     CDS             81615..>82692
                     /locus_tag="J8C37_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAZ5303553.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSR"
BASE COUNT        27035 a        13932 c        17511 g        24214 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13681 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13741 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13801 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    13861 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    13921 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    13981 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14041 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14101 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14161 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14221 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14281 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14341 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14401 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14461 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14521 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14581 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14641 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14701 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14761 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    14821 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    14881 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    14941 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15001 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15061 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15121 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15181 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15241 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15301 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15361 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15421 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15481 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15541 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15601 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15661 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15721 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15781 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    15841 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    15901 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    15961 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16021 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16081 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16141 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16201 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16261 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16321 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16381 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16441 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16501 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16561 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16621 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16681 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16741 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16801 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    16861 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    16921 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    16981 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17041 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17101 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17161 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17221 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17281 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17341 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17401 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17461 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17521 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17581 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17641 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17701 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17761 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    17821 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    17881 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    17941 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18001 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18061 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18121 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18181 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18241 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18301 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18361 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18421 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18481 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18541 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18601 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18661 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18721 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18781 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    18841 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    18901 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    18961 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19021 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19081 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19141 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19201 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19261 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19321 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19381 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19441 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19501 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19561 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19621 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19681 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19741 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19801 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    19861 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    19921 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    19981 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20041 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20101 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20161 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20221 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20281 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20341 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20401 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20461 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20521 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20581 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20641 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20701 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20761 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    20821 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    20881 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    20941 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21001 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21061 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21121 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21181 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21241 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21301 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21361 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21421 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21481 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21541 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21601 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21661 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21721 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21781 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    21841 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    21901 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    21961 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22021 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22081 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22141 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22201 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22261 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22321 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22381 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22441 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22501 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22561 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22621 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22681 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22741 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22801 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    22861 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    22921 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    22981 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23041 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23101 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23161 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23221 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23281 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23341 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23401 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23461 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23521 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23581 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23641 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23701 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23761 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    23821 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    23881 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    23941 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24001 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24061 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24121 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24181 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24241 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24301 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24361 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24421 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24481 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24541 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24601 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24661 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24721 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24781 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    24841 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    24901 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    24961 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25021 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25081 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25141 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25201 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25261 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25321 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25381 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25441 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25501 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25561 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25621 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25681 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25741 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25801 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    25861 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    25921 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    25981 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26041 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26101 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    26161 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26221 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26281 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26341 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26401 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    26461 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26521 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26581 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26641 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26701 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26761 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    26821 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    26881 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    26941 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27001 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27061 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27121 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27181 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27241 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    27301 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27361 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27421 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27481 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27541 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27601 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27661 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27721 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    27781 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    27841 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    27901 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    27961 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28021 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28081 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28141 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28201 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28261 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28321 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28381 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28441 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28501 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28561 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28621 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28681 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28741 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28801 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    28861 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    28921 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    28981 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29041 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29101 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29161 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29221 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29281 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29341 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29401 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29461 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29521 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29581 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29641 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29701 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29761 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    29821 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    29881 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    29941 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30001 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30061 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30121 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30181 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30241 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30301 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30361 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30421 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30481 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30541 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30601 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30661 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30721 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30781 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    30841 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    30901 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    30961 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31021 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31081 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31141 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31201 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31261 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31321 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31381 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31441 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31501 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31561 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31621 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31681 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31741 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31801 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    31861 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    31921 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    31981 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32041 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32101 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32161 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32221 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32281 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32341 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32401 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32461 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32521 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32581 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32641 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32701 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32761 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    32821 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    32881 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    32941 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33001 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33061 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33121 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33181 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33241 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33301 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33361 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33421 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33481 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33541 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33601 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33661 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33721 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33781 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    33841 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    33901 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    33961 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34021 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34081 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34141 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34201 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34261 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34321 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34381 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34441 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34501 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34561 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34621 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34681 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34741 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34801 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    34861 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    34921 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    34981 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35041 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35101 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35161 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35221 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35281 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35341 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35401 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35461 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35521 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35581 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35641 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35701 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35761 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    35821 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    35881 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    35941 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36001 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36061 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36121 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36181 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36241 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36301 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36361 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36421 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36481 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36541 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36601 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36661 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36721 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36781 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    36841 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    36901 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    36961 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37021 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37081 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37141 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37201 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37261 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37321 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37381 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37441 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37501 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37561 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37621 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37681 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37741 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37801 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    37861 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    37921 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    37981 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38041 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38101 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38161 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38221 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38281 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38341 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38401 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38461 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38521 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38581 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38641 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38701 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38761 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    38821 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    38881 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    38941 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39001 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39061 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39121 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39181 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39241 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39301 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39361 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39421 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39481 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39541 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39601 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39661 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39721 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39781 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    39841 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    39901 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    39961 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40021 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40081 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40141 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40201 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40261 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40321 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40381 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40441 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40501 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40561 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40621 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40681 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40741 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40801 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    40861 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    40921 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    40981 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41041 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41101 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41161 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41221 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41281 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41341 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41401 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41461 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41521 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41581 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41641 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41701 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41761 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    41821 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    41881 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    41941 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42001 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42061 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42121 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42181 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42241 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42301 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42361 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42421 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42481 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42541 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42601 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42661 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42721 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42781 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    42841 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    42901 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    42961 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43021 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43081 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43141 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43201 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43261 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43321 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43381 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43441 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43501 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43561 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43621 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43681 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43741 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43801 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    43861 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    43921 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    43981 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44041 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44101 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44161 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44221 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44281 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44341 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44401 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44461 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44521 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44581 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44641 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44701 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44761 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    44821 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    44881 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    44941 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45001 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45061 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45121 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45181 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45241 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45301 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45361 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45421 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45481 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45541 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45601 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45661 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45721 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45781 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    45841 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    45901 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    45961 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46021 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46081 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46141 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46201 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46261 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46321 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46381 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46441 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46501 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46561 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46621 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46681 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46741 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46801 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    46861 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    46921 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    46981 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47041 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47101 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47161 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47221 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47281 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47341 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47401 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47461 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47521 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47581 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47641 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47701 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47761 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    47821 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    47881 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    47941 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48001 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48061 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48121 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48181 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48241 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48301 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48361 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48421 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48481 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    48541 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48601 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48661 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48721 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48781 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    48841 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    48901 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    48961 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49021 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49081 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49141 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49201 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49261 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49321 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49381 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49441 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49501 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49561 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49621 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49681 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49741 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49801 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    49861 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    49921 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    49981 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50041 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50101 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50161 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50221 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50281 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50341 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50401 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50461 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50521 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50581 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50641 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50701 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50761 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    50821 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    50881 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    50941 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51001 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51061 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51121 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51181 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51241 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51301 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51361 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51421 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51481 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51541 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51601 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51661 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51721 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51781 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    51841 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    51901 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    51961 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52021 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52081 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52141 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52201 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52261 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52321 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52381 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52441 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    52501 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52561 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52621 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52681 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52741 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52801 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    52861 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    52921 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    52981 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53041 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53101 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53161 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53221 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53281 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53341 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53401 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53461 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53521 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53581 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    53641 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    53701 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    53761 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    53821 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    53881 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    53941 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    54001 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    54061 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    54121 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    54181 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    54241 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    54301 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    54361 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    54421 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    54481 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    54541 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    54601 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    54661 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    54721 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    54781 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    54841 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    54901 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    54961 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    55021 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    55081 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    55141 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    55201 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    55261 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    55321 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    55381 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    55441 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    55501 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    55561 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    55621 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    55681 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    55741 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    55801 aagcgatagg tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt
    55861 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
    55921 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
    55981 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
    56041 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
    56101 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
    56161 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
    56221 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
    56281 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
    56341 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
    56401 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
    56461 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
    56521 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
    56581 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
    56641 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
    56701 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
    56761 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
    56821 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
    56881 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
    56941 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
    57001 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
    57061 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
    57121 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
    57181 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
    57241 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
    57301 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
    57361 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
    57421 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
    57481 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
    57541 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
    57601 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
    57661 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
    57721 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
    57781 taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata
    57841 aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt
    57901 ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac
    57961 atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat
    58021 gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact
    58081 gttgagcata cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga
    58141 agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg
    58201 cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat
    58261 ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc
    58321 gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc
    58381 caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac
    58441 tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt
    58501 tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa
    58561 aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt
    58621 tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag
    58681 atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa
    58741 atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta
    58801 aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg
    58861 cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc
    58921 atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc
    58981 ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt
    59041 tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga attataccaa
    59101 aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg
    59161 tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt
    59221 aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca
    59281 taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt
    59341 cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga
    59401 atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc
    59461 tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc
    59521 tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga
    59581 agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg
    59641 tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga
    59701 ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg
    59761 agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt
    59821 taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga
    59881 cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat
    59941 tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga
    60001 ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga
    60061 cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt
    60121 atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc
    60181 acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc
    60241 agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg
    60301 tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga
    60361 tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac
    60421 taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg
    60481 gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc
    60541 agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga
    60601 tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct
    60661 tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg
    60721 caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta
    60781 attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg
    60841 tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga
    60901 aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca
    60961 gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt gtttctttta
    61021 gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca
    61081 aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag
    61141 ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat
    61201 tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag
    61261 taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta
    61321 tagggatctt gattgcttct cttattattt attttgtagc agatatacgt attgattcct
    61381 tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt
    61441 tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg
    61501 cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg
    61561 cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt
    61621 ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag
    61681 ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga
    61741 tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga
    61801 gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa
    61861 tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt
    61921 tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc gtattgatta
    61981 ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac
    62041 atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa
    62101 aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg
    62161 atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt
    62221 atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt
    62281 cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt
    62341 catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc tagcgattct
    62401 cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg
    62461 gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa
    62521 gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc
    62581 tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc
    62641 cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta
    62701 gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt
    62761 gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc
    62821 ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc
    62881 gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga
    62941 aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt
    63001 atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata
    63061 gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc
    63121 cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa
    63181 cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat
    63241 tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga
    63301 gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag
    63361 aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg
    63421 tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc
    63481 attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa
    63541 tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta
    63601 ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc
    63661 gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct
    63721 ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt
    63781 tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct
    63841 cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg
    63901 gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt
    63961 caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt
    64021 ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt
    64081 gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg
    64141 caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat
    64201 gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat
    64261 attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga
    64321 aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg
    64381 agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt
    64441 acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata
    64501 tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa
    64561 ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac
    64621 cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat
    64681 tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca
    64741 caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc
    64801 gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa
    64861 tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt
    64921 ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat
    64981 catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg
    65041 tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt
    65101 tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta
    65161 gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg
    65221 ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc
    65281 tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct
    65341 taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa
    65401 aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa
    65461 agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca
    65521 tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata
    65581 tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca
    65641 tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt
    65701 agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat
    65761 cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt
    65821 tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc
    65881 agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc
    65941 aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga
    66001 aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat
    66061 cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa
    66121 tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa
    66181 cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga
    66241 attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca
    66301 taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta
    66361 tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga
    66421 ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga
    66481 aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg
    66541 ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa
    66601 ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac
    66661 gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg
    66721 gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg
    66781 cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat
    66841 cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca
    66901 cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga
    66961 gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga
    67021 ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata
    67081 cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc
    67141 ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg
    67201 gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa
    67261 cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat
    67321 taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag
    67381 aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag
    67441 caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa
    67501 acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta
    67561 cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa
    67621 cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg
    67681 aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta
    67741 ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt
    67801 tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag
    67861 atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt
    67921 attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag
    67981 aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc
    68041 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
    68101 ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat
    68161 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
    68221 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
    68281 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
    68341 caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg
    68401 atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg
    68461 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
    68521 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta
    68581 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
    68641 catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc
    68701 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
    68761 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
    68821 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
    68881 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
    68941 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
    69001 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
    69061 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
    69121 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
    69181 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
    69241 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
    69301 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
    69361 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
    69421 tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt
    69481 gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac
    69541 ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
    69601 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
    69661 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
    69721 aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg
    69781 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
    69841 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
    69901 gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac
    69961 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
    70021 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
    70081 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt
    70141 gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
    70201 tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt
    70261 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
    70321 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
    70381 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt
    70441 tcctttggtt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
    70501 cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac
    70561 agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat
    70621 ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg
    70681 atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc
    70741 agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt
    70801 tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc
    70861 ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa
    70921 agtcaatttg tttaccgatc gagaagatat ttggcataaa ttctatcaga cgctaggaga
    70981 aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt
    71041 tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg
    71101 gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc
    71161 caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga
    71221 ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt
    71281 tcatcgatat gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga
    71341 cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag
    71401 gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc
    71461 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
    71521 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
    71581 aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat
    71641 ttgtatttat ttatgaagga catttatcag aacagaaaat tattgctaca gttttcgatc
    71701 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
    71761 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
    71821 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
    71881 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
    71941 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
    72001 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
    72061 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
    72121 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
    72181 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
    72241 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
    72301 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
    72361 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
    72421 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
    72481 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
    72541 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
    72601 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
    72661 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
    72721 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
    72781 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
    72841 ttgaatttgc tgacatcgga gaccatattt atcaacctgt aaaaacttat tcaagcggga
    72901 tgttcgttcg tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg
    72961 atgaagcatt ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa aaatttacag
    73021 aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatatcagt tcgattcgtc
    73081 gtttttgtga tcgttcctat tggttgaaaa acggagaagt cgtcgaatat ggcgatacga
    73141 tggaagtaac gacgaattat gaaaactttt taaagaaaaa atcagttaaa acagtggatc
    73201 ggaataaaaa ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg
    73261 aattgttgga taaagacaaa aatccggtgg atatgctgga acaaggagaa aaactatacg
    73321 ttaaagtaac ttatgaagta aaagatgata caatcaaaaa tccggtttta ggaattgcgc
    73381 tacgaacagt cgacaatttt tatgtatgcg gacttaatac tctactagat ggtatcaaga
    73441 ttccttggaa aaaaggagag aatatctttt atctggagta tgaaaagtta ggtttgttag
    73501 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
    73561 ataagacaaa gtacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
    73621 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
    73681 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
    73741 ttagagtttg gtcccggaaa cggccgatta acgaaacatt taatcggagc aaaacaatgt
    73801 gaagtgagca ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga atttgctcaa
    73861 gatggatttt atggtgatat cgaaagtttt gagtgggcaa actattatgc tggacagaca
    73921 tttgattacg tcttgtttgc ggatgtttta gaacatttgg tagatcctgg aaaaacattg
    73981 aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaacttg
    74041 gcgcataatt ctgtcatgat cgatttgttc aataaccaat tgccgtgggc ttcttatggt
    74101 ctcctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa
    74161 aaagcgggat tattcattaa tattgaagat tatttgtact tagctgtagg tgatacagaa
    74221 ttaaaatcaa cttatgaaga attgccggaa gctgtacgct atgatttcaa aatgcgacca
    74281 tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagc acaaagttcc
    74341 attgcagagc cacaaaattc taattatgta aaagtgttag aagtaaccca gcaaacgaaa
    74401 caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa tgagacacta
    74461 agtttccccg tatcagaaac aacagaaaga atggtgtttc gcttcgcgca acaacctagc
    74521 tttatcgaat tcagcgcaga agctgccgga gaaaaactaa catttatcga ttcgaatgct
    74581 gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaattacc agagttcgtt
    74641 ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa
    74701 ttgacaccaa caatgaaaga attgttggaa acgattcgtc ccatggctga agtcacaaag
    74761 cagctttctg agaaaaatga cgaattagaa agagaaaatc atcatcttct tgaggaaaat
    74821 acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt
    74881 gatgaatgga caataaaaca tagactagga cgacgtaaga aagaaacttc gaaaaaaatc
    74941 caagaaaaag aactttctat atgtattgac gagaaaatct gggatgcaga aacaaaaata
    75001 ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agcccttgtc ctataagtta
    75061 tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagaaga agtcaatcaa
    75121 gcagaacagc tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa
    75181 gaacgttcat tcttagtgga agcagtagct caaaatggcc aatcatggat cattgaaatg
    75241 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
    75301 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
    75361 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
    75421 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
    75481 agacccaaag cggtttcaag gtacgtttat cagggaaaat gagaggaaaa gccatcttag
    75541 acttccaaac agcgagacat caaaatggca tagctgtgaa attaaacggt aaataccctt
    75601 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
    75661 attatgcaag gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc
    75721 caggatcgat cgactatgca gagtggatca gtcgtcatga gaaaccagat ttgaaagagc
    75781 aaaggaaaga agcacaagga tttgcgtacc gtccattgat ttccatactt atgcctgtct
    75841 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtactggat caaacgtatg
    75901 atcattggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
    75961 tggaatccta tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac
    76021 acatcagtct ggcaactaat tctgcattgg aaatagcaga aggagaattt atagcactct
    76081 tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaacg
    76141 tacatccaga actggatgtg atctacagtg acgaagataa aatcgatgca gatggcaatc
    76201 gctttgatcc gcatttcaaa gcagactggt cgcctgatac attgatggga aataattata
    76261 tctctcactt aggcgtatat cgaacaagca ttgttaaaga acttggcggt ttccgtaaag
    76321 gctatgaagg ttctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag
    76381 atcatattta ccacattgac cgagtcttgt atcattggcg gacgatccca ggatcaactg
    76441 caagtaatgg cgaagccaaa agttatatct atgattccgg agtgaaagca ttgacagatg
    76501 ccctgtctag aagaaacatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg
    76561 agattgctta tgacgtcttg caagaggatt tggtcagtgt gatcattcca acgaagaatg
    76621 gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt
    76681 atgaaatcat tattgcggat aacggaagta cagatccaaa aatgcaagaa ttatttgcag
    76741 aatacaagca tcagctgaaa gaccgcttta tcgtagaatt gattgacatt ccattcaatt
    76801 attctcgaat taacaattta gcagcagaaa aagcaaacgg caaatacttt ttattcttga
    76861 ataacgatac agaagtgatt gagccagatt ggatgacggt gatggtttct tatgcacagt
    76921 ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg
    76981 caggagtatt attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa
    77041 cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
    77101 cttgtatgat ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag
    77161 aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg
    77221 tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata
    77281 ctccagaaaa acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag
    77341 catatattgc gcatgatcca ttttacaatg ataatttaac aaaagacgga atcggcgact
    77401 tttcgttgag accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca
    77461 taacggcttt ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa
    77521 gtcgaggttg ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc
    77581 ttttcaaatt tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt
    77641 tattcgtttt taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca
    77701 tgctcagagc gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa
    77761 agaatcgata ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc
    77821 tacaaaagcc actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag
    77881 attgttctta gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc
    77941 gttctcagaa tctaataccc accataaata gccatatctc atattttgta agtcttctaa
    78001 agcactggtt ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc
    78061 tttatacatc cctttattta ggcataatac accaattttt gccagatcac ttgccgataa
    78121 acacaaacca tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt
    78181 aggagactta tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac
    78241 atggtttggt atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag
    78301 cttacttgtc tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa
    78361 ttcgccggtt ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc
    78421 acttgaacag acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag
    78481 caagtgcttg attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg
    78541 aaaataatct aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc
    78601 aatcagcaaa gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt
    78661 ataaccattc cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca
    78721 tatatttggt tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta
    78781 cctcactttc ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt
    78841 cacgtaatac aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa
    78901 aatataaagg ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa
    78961 tttaccaaca ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt
    79021 gattagaggt gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat
    79081 aaactaggtt tttaaaggtg actatggtaa gataatagag atattatttt gtgattagga
    79141 gtgtatatat gaataaaaat ggagaatgga gtgctgcacg acggatattc attatattaa
    79201 tggatgttat tgtatacaat gcagcaatat atagtagttt cttactgaag tttcgtggag
    79261 agatcccatt acgaaattta gaaactttcc aacactcagc aattattatt tcagtgattt
    79321 ttattgcgtt gaacattttg ttgggtgcat atgtttttta taatcgaatg atcagtgaca
    79381 tcgtgtttgt cacagtcatc ggtcaggtat tgatgacact agggatcatg gtagtgactt
    79441 ttgcgggccg ctggtttgct tttccacgtc ttgttatctt actttcattt gttctcggta
    79501 cgcttttctt aagtatctat cgtattctcg tatataaact ttatttacgc gtaagtcgag
    79561 ataaaaaggt cgtcattatt gggttagaaa aggatgtcgc accagcaatc caaaattttg
    79621 gttcaaagaa aaacaacaaa cataaagtcg aagcagtcat tcttgataac tattatgcaa
    79681 acatcaaaaa tatgattaaa gacatcgata ttgtctatct tgcttctcat atcgaagaaa
    79741 ctgaaaaaat caaaatctat caattagtta ctaaaaatga aaaaaaattg tttttgaata
    79801 caacatttga aaacttgaca ttgatcaatc caaatattat gaatttcgaa gatgaaagta
    79861 ttattgaagc atctggattt agaatccaac ctgaatatga actattcaaa cgtttgtttg
    79921 atatcatcgt ttcacttgtt ttattagtta ttgcttcacc atttatgtta ttcacagcaa
    79981 ttttagtcaa agtgacatca ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa
    80041 atcaaaagga attttcaatc tataaattcc gctcgatgac cgccacagcc gaagcaaaat
    80101 ctggaccagt tttagctaag agcaatgatg cacgagtaac gcctgtaggt aaatttattc
    80161 gagctgtccg ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga
    80221 tcgtaggacc acgtcctgaa cgttctttct tcgtcgatca gttcaatgca gaaaaccctt
    80281 attactactt acggcataat gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa
    80341 aatatgtctc tgattacaac agtaaattac gttttgattt actttatatc aaaaaatatt
    80401 cattgatgct tgatttaaag atcttgctgc aaaccatcaa gatcctattt gataaaatgt
    80461 cctctcaagg acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt gcttttccga
    80521 atgaaaaaat ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa
    80581 tcgtaccagt atacaatgca gaagcaacta ttggtcgaac gattgatatt ctaaaagaat
    80641 tggattatga agagtacgaa gtcgttttag taaatgatgg atcaacagat caaacacagg
    80701 aaatcattca agaactgatt gaaaatgatg aacgttttga attgattact acgaccaatc
    80761 aagggcccgg attagcaaga aacgaaggaa taagacagtc aaaaggagaa tatctcttat
    80821 tcttcgatgc agatgacacg ccagtaaaaa cgatcttgtc agattatgat catttgctag
    80881 aaaagagtcc aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca atggacgagg
    80941 gaagaatcgt ttccgaaaaa gaaaacttag ttacagaatg tcactatcta actcacgaag
    81001 agtttataaa agacatgtat cagttaatga atgaacagtt gatgtatgtt gtatggaaca
    81061 aatgctatcg tagcgatatt ctgaaaaata accaaattct atttaaaact tataatagtt
    81121 gtgaggatcg aatatttaac ttacattatt atgaatactg tcatcaagtg atcatgaacc
    81181 caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac
    81241 ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta acagacgata
    81301 ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta
    81361 tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga
    81421 gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa
    81481 taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa
    81541 gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa
    81601 tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag
    81661 cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt
    81721 ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt gaaagattag
    81781 gaggccgtat ttttgttgta cctaatccta agaaaaatat ttttaaatat ttaatagaag
    81841 tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt tattttggcg
    81901 ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt gctcatagcc
    81961 atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa
    82021 ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa gctggtgaaa
    82081 gtctgtttca aaaccatccc tttgagatag ttcataatgg tattgattta gagctttata
    82141 gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca atgagtactt
    82201 ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat ttgttagaga
    82261 tttttttaga aatactaaaa atgaattctg attcttactt gattttagta ggtgaagggt
    82321 cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa agagtaaaat
    82381 ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt tttgtaatgc
    82441 cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca aaattaaaat
    82501 tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg gattttgtat
    82561 cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg tatgctaata
    82621 aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa atggagagta
    82681 tatatagtag ag
//
LOCUS       DADOOS010000002        64188 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.002, whole genome shotgun sequence.
ACCESSION   DADOOS010000002 DADOOS010000000
VERSION     DADOOS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 64188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.002"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(137..1528)
                     /locus_tag="J8C37_000085"
     CDS             complement(137..1528)
                     /locus_tag="J8C37_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAZ5303554.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="J8C37_000086"
     CDS             complement(1741..2127)
                     /locus_tag="J8C37_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAZ5303555.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="J8C37_000087"
     CDS             complement(2183..2806)
                     /locus_tag="J8C37_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAZ5303556.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="J8C37_000088"
     CDS             complement(2870..3538)
                     /locus_tag="J8C37_000088"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAZ5303557.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="J8C37_000089"
     CDS             complement(3550..3990)
                     /locus_tag="J8C37_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303558.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4721)
                     /locus_tag="J8C37_000090"
                     /pseudo
     CDS             complement(4049..4721)
                     /locus_tag="J8C37_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4906..5304)
                     /locus_tag="J8C37_000091"
     CDS             complement(4906..5304)
                     /locus_tag="J8C37_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAZ5303559.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5301..6074)
                     /locus_tag="J8C37_000092"
     CDS             complement(5301..6074)
                     /locus_tag="J8C37_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAZ5303560.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6071..6754)
                     /locus_tag="J8C37_000093"
     CDS             complement(6071..6754)
                     /locus_tag="J8C37_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAZ5303561.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6921..7808)
                     /locus_tag="J8C37_000094"
     CDS             complement(6921..7808)
                     /locus_tag="J8C37_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5303562.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7805..8674)
                     /locus_tag="J8C37_000095"
     CDS             complement(7805..8674)
                     /locus_tag="J8C37_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5303563.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8811..10097)
                     /locus_tag="J8C37_000096"
     CDS             complement(8811..10097)
                     /locus_tag="J8C37_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5303564.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10370..10948
                     /locus_tag="J8C37_000097"
     CDS             10370..10948
                     /locus_tag="J8C37_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAZ5303565.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10948..11877
                     /locus_tag="J8C37_000098"
     CDS             10948..11877
                     /locus_tag="J8C37_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5303566.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11874..13610
                     /locus_tag="J8C37_000099"
     CDS             11874..13610
                     /locus_tag="J8C37_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5303567.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13673..14509)
                     /gene="pheA"
                     /locus_tag="J8C37_000100"
     CDS             complement(13673..14509)
                     /gene="pheA"
                     /locus_tag="J8C37_000100"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAZ5303568.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14506..15015)
                     /locus_tag="J8C37_000101"
     CDS             complement(14506..15015)
                     /locus_tag="J8C37_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAZ5303569.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15008..16303)
                     /gene="aroA"
                     /locus_tag="J8C37_000102"
     CDS             complement(15008..16303)
                     /gene="aroA"
                     /locus_tag="J8C37_000102"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAZ5303570.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16316..17407)
                     /locus_tag="J8C37_000103"
     CDS             complement(16316..17407)
                     /locus_tag="J8C37_000103"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAZ5303571.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17424..18590)
                     /gene="aroC"
                     /locus_tag="J8C37_000104"
     CDS             complement(17424..18590)
                     /gene="aroC"
                     /locus_tag="J8C37_000104"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAZ5303572.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18592..19656)
                     /gene="aroB"
                     /locus_tag="J8C37_000105"
     CDS             complement(18592..19656)
                     /gene="aroB"
                     /locus_tag="J8C37_000105"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAZ5303573.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19689..20711)
                     /gene="aroF"
                     /locus_tag="J8C37_000106"
     CDS             complement(19689..20711)
                     /gene="aroF"
                     /locus_tag="J8C37_000106"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAZ5303574.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21370..22344)
                     /locus_tag="J8C37_000107"
     CDS             complement(21370..22344)
                     /locus_tag="J8C37_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAZ5303575.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22325..24208)
                     /locus_tag="J8C37_000108"
     CDS             complement(22325..24208)
                     /locus_tag="J8C37_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAZ5303576.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24226..26451)
                     /locus_tag="J8C37_000109"
     CDS             complement(24226..26451)
                     /locus_tag="J8C37_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAZ5303577.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26566..27600
                     /locus_tag="J8C37_000110"
     CDS             26566..27600
                     /locus_tag="J8C37_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5303578.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27667..28680)
                     /locus_tag="J8C37_000111"
     CDS             complement(27667..28680)
                     /locus_tag="J8C37_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5303579.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28758..28946)
                     /locus_tag="J8C37_000112"
     CDS             complement(28758..28946)
                     /locus_tag="J8C37_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAZ5303580.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29386..29514)
                     /locus_tag="J8C37_000113"
     CDS             complement(29386..29514)
                     /locus_tag="J8C37_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAZ5303581.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29517..30890)
                     /gene="rlmD"
                     /locus_tag="J8C37_000114"
     CDS             complement(29517..30890)
                     /gene="rlmD"
                     /locus_tag="J8C37_000114"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAZ5303582.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31001..32056)
                     /locus_tag="J8C37_000115"
     CDS             complement(31001..32056)
                     /locus_tag="J8C37_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAZ5303583.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32066..33496)
                     /gene="gatB"
                     /locus_tag="J8C37_000116"
     CDS             complement(32066..33496)
                     /gene="gatB"
                     /locus_tag="J8C37_000116"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAZ5303584.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33493..34965)
                     /gene="gatA"
                     /locus_tag="J8C37_000117"
     CDS             complement(33493..34965)
                     /gene="gatA"
                     /locus_tag="J8C37_000117"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAZ5303585.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34965..35270)
                     /gene="gatC"
                     /locus_tag="J8C37_000118"
     CDS             complement(34965..35270)
                     /gene="gatC"
                     /locus_tag="J8C37_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAZ5303586.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35376..37412)
                     /gene="ligA"
                     /locus_tag="J8C37_000119"
     CDS             complement(35376..37412)
                     /gene="ligA"
                     /locus_tag="J8C37_000119"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAZ5303587.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37429..39663)
                     /gene="pcrA"
                     /locus_tag="J8C37_000120"
     CDS             complement(37429..39663)
                     /gene="pcrA"
                     /locus_tag="J8C37_000120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAZ5303588.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39818..41377
                     /locus_tag="J8C37_000121"
     CDS             39818..41377
                     /locus_tag="J8C37_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAZ5303589.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41488..43419)
                     /locus_tag="J8C37_000122"
     CDS             complement(41488..43419)
                     /locus_tag="J8C37_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAZ5303590.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43437..44351)
                     /gene="pfkB"
                     /locus_tag="J8C37_000123"
     CDS             complement(43437..44351)
                     /gene="pfkB"
                     /locus_tag="J8C37_000123"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAZ5303591.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44348..45100)
                     /locus_tag="J8C37_000124"
     CDS             complement(44348..45100)
                     /locus_tag="J8C37_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ5303592.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45259..45468)
                     /locus_tag="J8C37_000125"
     CDS             complement(45259..45468)
                     /locus_tag="J8C37_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303593.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45479..46807)
                     /locus_tag="J8C37_000126"
     CDS             complement(45479..46807)
                     /locus_tag="J8C37_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303594.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47172..47759)
                     /locus_tag="J8C37_000127"
     CDS             complement(47172..47759)
                     /locus_tag="J8C37_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAZ5303595.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47842..49092)
                     /gene="clpX"
                     /locus_tag="J8C37_000128"
     CDS             complement(47842..49092)
                     /gene="clpX"
                     /locus_tag="J8C37_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAZ5303596.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49339..50628)
                     /locus_tag="J8C37_000129"
     CDS             complement(49339..50628)
                     /locus_tag="J8C37_000129"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAZ5303597.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50782..51714)
                     /locus_tag="J8C37_000130"
     CDS             complement(50782..51714)
                     /locus_tag="J8C37_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303598.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51871..52530)
                     /locus_tag="J8C37_000131"
     CDS             complement(51871..52530)
                     /locus_tag="J8C37_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAZ5303599.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52533..53558)
                     /gene="sppA"
                     /locus_tag="J8C37_000132"
     CDS             complement(52533..53558)
                     /gene="sppA"
                     /locus_tag="J8C37_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAZ5303600.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53571..53861)
                     /locus_tag="J8C37_000133"
     CDS             complement(53571..53861)
                     /locus_tag="J8C37_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303601.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54270..56075
                     /gene="glmS"
                     /locus_tag="J8C37_000134"
     CDS             54270..56075
                     /gene="glmS"
                     /locus_tag="J8C37_000134"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAZ5303602.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56147..57502)
                     /locus_tag="J8C37_000135"
     CDS             complement(56147..57502)
                     /locus_tag="J8C37_000135"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAZ5303603.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57526..58695)
                     /locus_tag="J8C37_000136"
     CDS             complement(57526..58695)
                     /locus_tag="J8C37_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303604.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58692..59579)
                     /locus_tag="J8C37_000137"
     CDS             complement(58692..59579)
                     /locus_tag="J8C37_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAZ5303605.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59768..63460)
                     /gene="nifJ"
                     /locus_tag="J8C37_000138"
     CDS             complement(59768..63460)
                     /gene="nifJ"
                     /locus_tag="J8C37_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAZ5303606.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63659..64180
                     /locus_tag="J8C37_000139"
     CDS             63659..64180
                     /locus_tag="J8C37_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAZ5303607.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17837 a        13920 c        11457 g        20974 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaaattt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgttttttt actgatcact
     4321 tttaaaaagc tttttagaat cttttgctga cgtctaaaaa tagaaaaaga attttcgtca
     4381 tctatttgat aacgcatttg tttttgcagt tctgctgcag tcagatggat cttcccatcg
     4441 tttgtatcga tcattaaacc ttctggaaac agttcaacca ctgctcgatc ccaagattca
     4501 agagaaagct cgatataatc ttttactggt aattccaatt ctaccttaaa aaagtgcaaa
     4561 acagccgaca gcccttctgc ttgatagatt tctcctaatg ttagcccctc tagatggaac
     4621 tctggtgaaa aatgcagcaa tgttttattt tcctgcaatt gaacatatag aaatgtagat
     4681 cgctcaatca gagtatcttt taaaacaact aaaaaattca tttttctccc ccttttttca
     4741 tcatttttaa aatagcatat tttgaaaaga aagtctgatg cttagagtag aatttcataa
     4801 aaatttacta tttcataagt attcacgaga aaaatacaaa caaaaaagcg gaaataacgc
     4861 tcatccgcct tcttgtttgt gtttatttcc tcttcaaacg cttgtctatg attcagactt
     4921 ttctctatcg ttattttcat ctaaaagttc ttgcattccg ataatttctc gatattgatg
     4981 aatcttagtg ataccaatga ctttgtctac atcaatacta aaacaaatac cattacctgg
     5041 aatatcgatg ttgatgcctt gaccaatcgc atccattact ttattcgtca aactatcggg
     5101 tactaatgtc aacacaatat ctttttctgg cgaaatgtcc aaacctaaaa atttccctga
     5161 atcatgtaca ccagaacctc ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt
     5221 tgaaaagtcg ataacatctt cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag
     5281 attcaatgct acaagatctt tcatttgctt tcccccaatt tttgcaatct ttctttttcg
     5341 attctgtctt tttcatcttt tcgtttataa atatatccta gtactaaagt ggataatatt
     5401 ggtgtcaatg caatcattgc aaccacacca aacccgtcaa tgatcggatc tcttccttca
     5461 atttgtgcag cgattgccac gctcattgat aaaataaatg tcgaacacat cgggcccgtt
     5521 gctactccac cgttatcaaa agccattgct aaaaagatct tgtccacttt tcgtcctaaa
     5581 ataaatgcta gcccgtatcc tgggatcaag aaataataaa gagagaatcc gacgagcaaa
     5641 cgccacatag acaatcctac tgctgcgccg atcccaattg aaattaccat cagcatgatt
     5701 ttcgatcgga ttctcccctc gctcaattct tcaacctgtt tgatcattac atgaatcgcc
     5761 ggctcagcaa accctactaa gaaccccatt aaaaagccaa ggggaaccag tatataattg
     5821 taaggcaatc ccgcaatagc actgcctaaa tgctgtccta ccggtacata agccatattg
     5881 actccgtgca ggaaaaggat caacccaata gtcgttatca agaaaccttt cataatgtca
     5941 aagaaacgct ttttgcgcat acgaaaacta atgatattca ttaaaacaaa taggattacg
     6001 ataggtgcca ctgccaaaat gacttccatg gtaacgattt cgattccatc tatcacttgc
     6061 tgccagaaac tcattggaag atcaccccca aaatcatcac ggcaagaatc ggccctaacg
     6121 aagcaatacc gaccattcca aaactgtcat tttcattttt cttttgtttg atcatactag
     6181 tcatccctcc ggcaagggcc aagataaatg gaacagttac tggaccagta gtcactcccc
     6241 ccgaatcaaa agcaatcggc ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc
     6301 ctatgtagcc tatagccatc aattgataat aggatagctt gaatacgacc cgaagcagcg
     6361 caaaggctag aaatactcca gtgccgacgc tgacgatccc aatcagcaac actctgccaa
     6421 tctctccgtc agatatatca tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa
     6481 tcgtgatcac gatcccgata gcaaatccca aactgaccaa aacggtcaaa cttttctttt
     6541 tgatcatata tttccctact agatctccga cttccatcat agaataatct gctccaaaca
     6601 aaaagagcgt cattccgatc atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt
     6661 ctccttctaa cggtgcaaaa acaaacgtta aaatgacaat caggacagtc atcggcaata
     6721 tagcagcaat cacttcttta aaatttttta gcatgtatcc tcctccttta ctctttaagt
     6781 ttaccttaaa atgaaagagt tttcgagcac aaacttaaaa cgttatcatt taatttttct
     6841 caacataaaa taacacacgt cccaaaagta tctttttact tggaaaaatg actttgaagg
     6901 aacgcgctta ttgtgttttt ttattttaca gagccgatca ttccttgaac gaattgcttt
     6961 tgcaaaacaa aaaatacgat ggcagttggt aaagtagcga tgactaatcc agccatgatc
     7021 attccatagt ctggtgtata ggaagcaccc atagcagata aaacaagcgg aaccgtccgt
     7081 ttatcaggag attgcaaagc tactaagggc cataaataat tgttccagct actcatgaat
     7141 gtgatgattg cagccgcggc ataggtattt ttagccgtcg gcatatacac tctaaaaaaa
     7201 atacttagtt cttttagacc gtctaatcga gcggcttcta ctagatcttt tggaaaagat
     7261 ttaacatttt gtcgaaagaa gaagatcaaa aaagcagtgc taacagatgg caggatgaca
     7321 accaagaaac tgttgattcc caaaaccgtt ccgttcaatt gtgaaaacat tcgatataaa
     7381 gggatcatca atgccgcaaa aggaaccatc atcgatagca gtagaatatt gaaaattagg
     7441 tcttttcgtt tactttgata aatctcaaat ccatatcctg ccattgacga aatcagcaat
     7501 gcaaacacag tggtcaccaa agcgatcatc gctgaattcc aaagggaacg agtaaaattc
     7561 agctcattgg aaaacaaatt ttgaaaattg atgatcaagt tactgccaat ctttattttt
     7621 cccgatgtga tatccacggg tgtattcgtc attcccaaaa ccatccagac aaaagggaaa
     7681 atggaaataa atgataggat gcttaatgct gtatacttta atactgaccc aattgtcgaa
     7741 cgaagttcta atgcttgttt tttgacgttg gggcgttcga ttgtttcttt ttctaccata
     7801 tttttcatcg actttctcct cctactttcg tttggattat cgacaatatc acaataaagg
     7861 aaacaataac aaaggaaact gctgccgcat atccaaaatt aggcgtgaat ttataactca
     7921 aattatagat gtattgagat agggtggtcg ttgcatttgc tggtcccccg ccagtaatat
     7981 tttgtacttc atcaaaaagc tgcaatgtcc cgatcgtcga agtaatggaa gtaaacaaaa
     8041 tgattggttt taaatttggg atcgtcactt tgataaactg ctgccattta gaagcaccgt
     8101 caatagatgc tgcttcataa atctccgaat caatattttg catgcctgaa aggaaaaaaa
     8161 tcatattgta tccagtccaa cgccaagtga tcgaaagaat gatcagaact ctcgcccaaa
     8221 ttggatgagt caaccagcca atcggtgtgt caatcatacc aatgcccaac aacaagttgt
     8281 ttacaaggcc agattgagaa aatagacttt tcatgatcag agaataactg accatcgaag
     8341 tgatacatgg aagaaagata gcggttcgaa acaacccttt atatttcaac tttttatcat
     8401 ttaaaaggtt ggaaataaaa agtgctagaa acaacatgat cggtacttgt acaagtaaat
     8461 acaggaatgt attaaaaaaa gcttttttaa aagttgcatc tgtcaacatc cgctgataat
     8521 tacctaaccc gttaaatgtc atattgacag ggggaccact ttgaaaagac gtgatcaaag
     8581 ccaaaaccat cggaataaaa accatgattg atatcaaaat gactggcaag attaaaaaag
     8641 catgcccgtt tttgaataaa tctttctgct tcattctttt ccctccaatt ctatcattga
     8701 ttatctacat gaaaagacaa gaaagacaat atctgtaagg cagacgtgat cttttttgtc
     8761 ttttcgtttg tctgatgaaa aagcgaagaa attcactttt ttaccaccga tcaattagcc
     8821 aattgtgctt ctacttgttt ttgtgtatct gctagtacct tgtctgtctc ttctccattg
     8881 atgattcgat gaagagcttc agctacgact gcttcaattg cataggtctc atgtccgtaa
     8941 ttcacttctg gtatttcaga tgtccatgtt gaaaaatcgt caaagatctt ttgaccactg
     9001 taaaattcag aagcttcttg ataagcttct tgttcattcg cgctaagcat cgtagatact
     9061 agtccgattt ctttcgcaag agtgcccatc agttcttcat tcgaagcaaa cgttgcttct
     9121 aggaaatctt tcgctgaatc ttctccagct actcctttga tcacatacca gccagcgccg
     9181 ccaaggttag aagcttgcgc tttttgcaga ttttctggaa gtgcaggtat tggtgcaatc
     9241 ttccattttc cagattggtt ttctgctcct tggatcgttg aagaatacca agcgccagtt
     9301 gggctagaag ctactgctcc tgattgtatc gcagttactc cagcattcca gtcagaggtt
     9361 tgttcgacta gatcttcttt caaaagagtg gcaaacaatt caagaccata ctttaaagat
     9421 gcattgtctt ggatcgtgac tgtctcccca tcttcagatg tataccattc tcctgcctgt
     9481 tgcataatca tccttactcg accaaggtca gaaggattga cttcagcgat tttctttccc
     9541 gtcttttctt tgacatctcg cgctacttga atatactcgt cccacttcag attctccata
     9601 tcctcttctg tataacctgc ttctgccatc agatccgtac gatagaagtt tgctgttacg
     9661 cccgaatcga aaggaacacc gtatatttta tctcctactt tgtttaccgc aaatttatac
     9721 ggtgcaaaat gatcttcctt tacaatagca gacaaattag cgaatgcatc tggataagaa
     9781 gtcaaatagc cttgtatcct ataatcttca atcaagacga tattgggcaa tccttctgta
     9841 ttcccgctcg ctaaagcggt gttcagtttc tgaacgatat catcctgaga catcgtaaca
     9901 acttctatct ctgtttcttc gttctcataa actttttttg cttcattcac tgctttgata
     9961 ttgaatgtct catcccacgc ccagacagtg actttttctt ctttgtctgc tgtactacta
    10021 gcacttccgc atcctgttaa gaaacctgat gctgccgcta aacaaacgac tccagcaatt
    10081 aactttctga tgttcatttt tatccgctcc ttttccatcc attagttgtt tacgtttgct
    10141 acattcatca taccaaaatt tgaaaacggt ttctttatta ttcagatact tcaaaaattt
    10201 ctcctaaaat aataaaaaaa tacaaatcaa ctatgacaaa aggaataatc ttagcatcta
    10261 gaccaaagat cgaaatggtg aggtttcttt aaaataaaaa tatccttgca ttttctccta
    10321 gaaataagta tttttaaatt acaagactat tcttaaggcg ggtaaaaaaa tgccaatacg
    10381 cgtaattctt atcgatgatg aacagacgat tttagatgga atgaaacagt tgatcaattg
    10441 gtctgagcac gggtttcaat taatagggac tttttccagt ccgctgaaag cgcttgattt
    10501 ttctttggta catgcaatcg atatcgtggt cacagatgta atgatgcctg aattaaatgg
    10561 aatcgaatta tcgcggctat taaaacagca acaacctgaa acacagatcc ttatcctctc
    10621 tagctatgat gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg ctgattatct
    10681 tttaaagcct cgattgagtc ctgatttttt tctaaattcc ttaactgccg tttcacaaaa
    10741 aatcaaaaaa aagcccaaca aaagcccaat caccaatcgt tatgagcaga tctgcgagaa
    10801 tctttcacag cagattgctg gacaaactcg tccattgctt gaccctcttc tttttgaaca
    10861 caccttgttt ttctgttata taccgaaaaa cggccattca gttcaaaaca aggctctaaa
    10921 caaaaacagt cgatcaccca ttcattgatg gatagcgacg attcgtttac tatttatcct
    10981 gtttctacct tttcggaaga atccggctat gtaatcaata ctgattccaa agatacactt
    11041 aggaagtttg ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt tgttgtttct
    11101 gattcttttg caatccaaga tatcaatgaa atattccaag agatcagaaa ttgcaccaaa
    11161 ggacaacgtt tctatcataa gggaaagaac cttgcttatc agcatgaact tcttccgtta
    11221 ttaaccaacc acgagcagca agcaagtagt tatttaacaa aaatcgttca taaagactac
    11281 gctttagctg tcaaagaact tgagaatttc ttcacgacga tattggaaca gcctattgat
    11341 ccagcttatt tgaaacatga ggcaatcaat cgtttctatg cattactcaa cgctttggct
    11401 gacgagcatg ttgggaatga agaactttct ttgctcaaga tcgcccttcc ttctttatta
    11461 gccgatgctg agtatttcga agatttttct attcttgtca aagaaacact tgagaaactc
    11521 acccactttt ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt gatctacgag
    11581 ttcgtccagg aaaattatga gaaagaaatc aatttacaac ttttatccga aaaatatcat
    11641 ttttcatata gctatctctc agccatcttt actgaaaaat ttggtatcaa cttttcaaaa
    11701 tatctaaaaa aagtccgtat cagtaaagcc aagctctttt tgacggaaac acggcttact
    11761 ttgacggaaa tctgtgagaa agtcggctat accgaactgg gttatttctc cagagttttt
    11821 aaagaagaga caggaatgac accttctcaa taccgtaaag gaacttttgt aaaatgagaa
    11881 aaaaaatcaa agagcatgaa ctctttgcca aacttctgtt cattttgttc atcggtcttt
    11941 ttctgcaagc catcgtcatt tctttgttta tttatcatcg atcaagggat gcctatatcc
    12001 agctgttcaa tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa tttgaaagtt
    12061 tgaatgatac aatcgagaat acattagccg cttttgattc taatccagct gtcaaaagct
    12121 atttttccaa tgatccagcc caacatatgg aacagcttca gcaactaaga acaatccaaa
    12181 aaatgaatga ctctctttca aaaatccatc caatgatcga ttacgatgta ttgatctttg
    12241 gagaaaatgg aagaactttt gtcggaaacg acatgctcac tgccgtatct gccgattctt
    12301 tttttcaatc tgcgattgct cagagagtga atgaacgtgc tgctgataca caaatgcttt
    12361 ttgcacacca cggattgact cttcgcgata agaaagcacc cagcgtcttc tttgtcaaaa
    12421 aactgaaaaa tacattgaat cacgtttacg gatatgctgt tttatcgatc tcatccaagc
    12481 aactagcgaa tctttttcaa tctgtagtaa atcctgagat atcgaagatc tccttcatga
    12541 acgaagaaca gacgatcatc gcatcaaatg aagaaaatac aattgggagc cgatcaatga
    12601 ttttcaaaaa actaacagta ggtgaaacaa cgatttcaaa tggacagcag ttgacccgtc
    12661 ttcctctgta ccgccaggat tgtacattac tcagtcaagt cgatcttcat tctttatcta
    12721 accaaatggg cgtgattttt ccgattattt tgtataatat cgtgacactt gcttttgctg
    12781 gctcattggt atttatgtat ttggatcgac acacaaaatc gatctaccgc ttgattgatt
    12841 cgctaaaacg gctagaggaa gatggcttaa aagctactgt tcctgtacaa ggaagccatg
    12901 aagtccaatt gctagggaat acgatcaatc aattgctaag taatgctcag catcattatg
    12961 aacagacgat ccaagacgaa aagaagaaac gttcgcttga aatccaaact atgcaagcac
    13021 agatacagcc ccattttatt tataatacgc tgactagctt gaaatttttg atttggcaga
    13081 aaaaaacaga tgaagcagtt caaggaatcg acagtttcat tactttacta agaagtacga
    13141 ttgggaaaaa agaagaagtg attccagtca aggaagaaat caaaagcgta caaagttaca
    13201 tcgatctttt gagtatgcga tatggcgaaa gaatctcggc taaaataatg attcctgatg
    13261 aactgcagtc attatctatg ccgaatatga tccttcaacc aattatcgaa aacgcctatc
    13321 ttcatgcttt tcacaataaa acgcaaggat tcatcacggt gtacggcaaa acaaaaaatc
    13381 agcttctttt attcgaagtg attgataacg gcgatggctt cgatactgaa aataccaaac
    13441 aaaaaaatga tcatttttca ggaatcggca tcagccatgt agatgagcgg atacgcctaa
    13501 tgtatggcga tgcatatggc ttaacgatcc aatcagtgat tgggacagga acgactgtaa
    13561 cgatttgtct accgcttctt cctaaaaatc aatcagaata tcaaaaataa agaatagcca
    13621 agcgctgatt tttttatttt aagttaaata aatcaatcag tattcttgac tttcatctac
    13681 gcgtctcttc tactgtcagc actggataac agcccaatat ttgactttct cctccgagca
    13741 tcttgatttc ttggagcgca ttctctacta atgtcattgg acggtccaac aacaaatcga
    13801 tcacgaaaaa atattctccc aagctagttt tcaatggtcg ggattcaatc ttacttaagt
    13861 tgatttcccg ccagccaaaa gctgccagca ttttatgaag catccctggc cgattagcag
    13921 gtagtgtcaa gatgacactc atttttactg gagctgactg ctggctggtc atctttcggt
    13981 caccgacgat ccaaaaacgt gtctggttca actcattatc ttggatattc tcagctaaga
    14041 tctccaatcc aacatgctgt gctgtttcta atgaagcaat cgctgctgcg tcttctttcg
    14101 gatgttctgc tacatacatt gctgcagcag ttgttgattc tgttgctact aaaggaacat
    14161 taggataatg cgtttctaaa aactgttgtg attgtgctag tgcttgtgga tgtgaaagga
    14221 tttttgtaat ctttgttgct ggattgccta aaagctgctg cttgatagga agaacgattt
    14281 cacttttaac ttcgacttct ggatgtttgg aaagaagatc gattgtgtga tgaacagctc
    14341 cttccaatga attctctaca ggaacgacag ccagatcgac ttgtttgcga aacaaagcgc
    14401 gcaaacaaac agggatggaa gcatacgcag tcaattgttc tgtaggaaat agctgacaag
    14461 ccgcctgata agtgaatgaa ctttctggcc ctagatagct gacgttcata tgcttttgat
    14521 cctttctata atggtatcga tgatcttttc tggtgtttga ttggtcgtgt tgattcgatg
    14581 tgtcgcactt tcttcatacc aagaaagtcg ttgtgccaat aatgctttta tttcttctct
    14641 tgtttttcca agagctaaag gtcgaataga cgtttcatct tgttggatac gatcaactag
    14701 acagtccgct tctgcctcta agtaaagaac aatcggctgt gtttttaaaa aacgccgatt
    14761 ttctgcactt tggacaatcc ctccaccagt cgcgataatt ttgtcttcat ttgacagctt
    14821 tcttaaaata tctgactcca cttttcgaaa gcttttttca ccgtaatagt gaaaatattc
    14881 ggcaatcgac aattggatat gttcctcaat ctttgtatct aaatctacat actctttttg
    14941 taaacattga gctaaccctt taccgattgt tgtttttcca gcgcccatga aaccaatcag
    15001 caaaatgcta gccatgagct tctcctcctg acacaagttc tgctaaatcg tcaaaaaatg
    15061 ccggataaga gattgaaaca gcttcagctc cttctagttc gcaaggtcca tcagctatca
    15121 aagcagctac ttgcagcatc ataccgattc gatgatcgcc tctgctggac acttttgcgc
    15181 catgcagagg tgtcggccca tggatgatca gaccgtctgc tgtcgctttt atctttgctc
    15241 ccattttctg taattcttct gccaccgcat caattctgtt ggtttctttt acttttaatt
    15301 cttctgcgtc tttgatgatc gtgattcctt ctgcttgagt agccactaaa gctagaatgg
    15361 gcaattcatc aatcagacgc ggaatgattt ctccaccaat cgttgctttc ttcaaatgac
    15421 tcgtcttgac acttaaatca gccgattggt tctgttcatt gtgattcgtc tgggtaatcg
    15481 ctgcgcccat ctcctccaac acatctaaga taccagttct tgttggattg accccgacat
    15541 tttttaacaa cagttggctt tccggaacaa gaagtcctgc tgctagaaag aaagcagctg
    15601 atgaaatatc acctggaaca gttatctgtt gaccagttaa tttttgcggt ccggtcacta
    15661 tgatcgtttt atcttccact gtgatccttc cgccaaattg acgaatcatt tcttccgtat
    15721 ggttacggga acgttctttt tcaacgatta cggatgtgcc ttctgcttgt aatgccgcaa
    15781 acagcaaagc agatttgact tgcgcactgg ctacaggcat attgtatcgg ataggtgata
    15841 aagagcgggt tcctgtcaca gtgattggta gatactggtc attttcttct ccttgcaagt
    15901 cagcgcccat ctcgcgtaaa ggttccatca ctcgccccat tggacgttta gacaacgaag
    15961 catccccgaa cagagtagtc gtaaaaggct gtccagccaa aatacccatc aataaacgta
    16021 tggtcgtacc ggagtttccc atgtcaattg attcggttgg ttggacaaat gagtggctac
    16081 cgcgaccatg aatcttgatc acctcttcct cttcttcgat ctcgacacct aattgttgga
    16141 aggcatgtaa agtactcaga caatcttctg cgcgcaaaaa attttgtata gtcgtcgttc
    16201 cttcagcaat tgctccaaac atgatgctgc gatgggaaat tgatttatct gctggtatcc
    16261 taacagtccc tcttaatcca tgtatttgtt gtaataattg catttatcca gcctcctatt
    16321 tgatccagca atgataactg gtattagaca taatgattct ttttgcttgt aacaaatcct
    16381 gttggttttt aaaactgatt tgcagtattc ctaaaatatc ttccctggtc tcttggatct
    16441 tcaaattaat caaagaaact cccgcttttc cgatcagtcc agttacttct gcaataacgc
    16501 caggttcatc tggcacatcg ataaataaat caaaaaaggc aggaatcgcg ccttgcttat
    16561 ggacaggcaa ctggtctcga ctttcttttg cccgattgaa aaaagaaaag atttccgatt
    16621 ggttctcagt catgatccat tctgctattt gtgtcatttc ttgttgccaa tctgccaatc
    16681 gttccaataa tatctgctta ttgctcaaca aaatatctgt ccacatgaca ggatctgagg
    16741 aagcgatcct agtgatatct cgaaagcctc ctgctgccag ttgtttggct cgcggatact
    16801 cttgattgaa ttgttctgcc tgattgacta agccagctgc cagaatatgt ggaaaatgac
    16861 ttagcattcc tgttatacgg tcatgttctc ttgctgtaag agttacatat tttgctttgg
    16921 ttccgcaaaa tagttcttgc agtttttcaa catctttttt cgtcttttcc aaaggtgtaa
    16981 agatgtaata ggcattttca aatagtttag catctgctgc ctgtatacct gatttatgag
    17041 aaccagccat tggatgtcca ccgacaaacc gaagattttt ctgctcagcg gatgtcacga
    17101 cttgctgctt cgtactgctg acatccgtaa tgatgacgtg atcagacaag ttgattcttt
    17161 caaggacatc aagataagct aaggttgtac ggacgggaac ggctaataag ataacgtctg
    17221 cttgttctgc acctgtttca aaatcacttg caacgtaatc aacgatctgc tgttctactg
    17281 cttgctcacg ggtttttgac tgactatccc aaccaactac tatcagttct ggattggtct
    17341 gacgaataca aagtgccata gaagcaccaa tcagtccaag ccccacgata aaaacatgtt
    17401 gtgccatctt atttgccccc tttttagtaa tgtttcaagt agtcacggta ctccttcact
    17461 gcagctttca actcaacaag cgaatcactt gggaattttg acaatagctc tgtcgctacc
    17521 acagtggcta caacattttc cgcaacgacg ctagctgcag gaactgctgt actatccgaa
    17581 cgttctacac ttgctttgta aggttctttt gtatcgatat caacactttg aagtggcttg
    17641 tacaatgtag gaatgggttt catgaccccg cgaatgacaa gtggttcgcc attggtcatt
    17701 ccgccttcga agccgcctag attattggta cgtcggaaat agcctctttc ttcttcccat
    17761 aggatctcat ccatcacttc tgagcctggt aatgaaccag aagtgaatcc gaccccaaat
    17821 tctacgcctt taaaggcatt gatactaacc acagcttgtg cgattttggc atctagcttc
    17881 gtgtcccatt gtacgtagct tcctaatcca gctggaacgc ctccaacgag cacttctaca
    17941 attcctccga tcgtatctcc atttttcttt gtctgatcga tcaaatcttt tattctttgc
    18001 tcaactgctg gatcaacaac tcgtacttct gacgcttcag aaatttcttt gatttcttga
    18061 atcgagatat ttccaggtat ttctgcttgg attccaccaa gacaaatcac gtgtcctgct
    18121 acgatgatgt caagttcttc cagaattttc ttggcaattg cgccgattgc tacacgcatg
    18181 gtcgtctctc ttgcagagga ccgttcaagc acattccgaa gatctcgatg atggtatttc
    18241 ataccgccta ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg cagtgattgg
    18301 tctttttcag gcacttctgc tacagacatg acagttgtcc aatttttcca atctttattt
    18361 tctaccacta aagtgacagg tgaaccaatt gttttgccgt gacggatgcc agacgtgatc
    18421 cgaacacgat ctttttcgat cttcatcctt cctcctctgc cgtaaccagc ttgtctgcga
    18481 gcgagttcgt gattgatttc ttcttctgac agaggtaatc ctgctggtag tccttcaata
    18541 atggctgtta attctggtcc atgggattct cccgcagtca tataacgcat cttacagtcc
    18601 tcctaagtaa tctttgaatg tctctgtggg aacaggaacg atttttgctt gcccaatctt
    18661 ttctagcaag atgatattca attggcttcc tcttgctttc ttatcatggg ttatcgcgtg
    18721 gaataattca tctggatgac ttagatcgta cgtaatcggc agatggaatt tttgaagtat
    18781 cctttctaaa gcagctgtcg atccagttgg agacaagccg attttttctg catgagaagt
    18841 gatggctacc attccaatag ctactccttc tccatgactg actaccccat atcctgctgt
    18901 attttcaatt gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat ttccttggtc
    18961 aaagacatct tcttccacta cttttcgttt gatttccaac gtagcagtaa tgattggaac
    19021 agcatgttcc agaagatctt tttcgtcttt cagctcgttc agctgtttcc aaagcgttgc
    19081 atctgctata gctgctgatt tgatgatttc agcgattcct tctcgtaaac gacgtaccgg
    19141 caaagttttt agaacctcag gatcaatcaa tacaccagct ggctggctaa atgttccgac
    19201 aaggtttttc gctttgtttg tattgactgc tgtcttcccg ccaatactag aatctacttg
    19261 tgcaagtagc gtagtcggaa tttgaacgaa atggatccca cgcatgaacg tcgaggccac
    19321 gaatgctgct agatcaccca ccattcctcc accaagtgca agtatcccat cgctgcgggt
    19381 aaaatcatta tctgctaaaa aatcgtataa ctcagctgct tgttctaaag atttactggt
    19441 ctcacccgcc ggtacgattg aaaggactgg ttcaaaatta gcttgtctca attggaacat
    19501 gattttttct gcatatagtg gagcaacgtt tgtatcagtg atcacagcta ctttttgagg
    19561 tctccaaatc gtttgaagcc atgatccagc ctgttctagt gcgccttttt caatatgaat
    19621 atcgtatttt tggttatcaa catgtacagt aagcattcgt ttcacttctt tcttttaaaa
    19681 agctggaatt aatcgagtgt ctgaatcttt tccattgctt ctttcatgat cgcaacttct
    19741 ttcatcatgc gtgagtaagt cttttcattc aacgattggg gaccgtctga ccaagcattt
    19801 gcaggatcag gatggatctc tacgatcatg ccatctgctc cgcttgccac acccgcacga
    19861 gccattggtg gtacaagttc ccaaattcct gtaccatgac ttggatcaac aatcacaggg
    19921 aaatgagtta attttttcat caatgggaca gcacttaaat caaacgtatt acgtgtcgct
    19981 gtttcgtaag tccgaatccc acgttcgata aaaatgacag gagatttgtc ttcgactgca
    20041 atgtattctg cagcattaag ccattcattg atcgtaccgg aaattccacg tttcaatcca
    20101 acaggtttac ctgttttacc gacagcagat agcagtttaa aattttgcat attacgtgcg
    20161 ccaatctgta ggatatcgct gtactcagcc accatatcga tatgcccttc atccatcact
    20221 tcagtaatca ctttcattcc aaattcatca gccgcttgac ggatgtattt caatccttct
    20281 tcttctaaac cttggaaagc atatggagaa gtacgtggtt tgaaggcacc acctcgcaga
    20341 atttttgcgc ccccggcttt ggccattcgc gcacattctc tgatttggtc taatccttcg
    20401 atagaacaag gaccagccat cgtcacgaaa ctgccgtcac caatttttac tccgtctaca
    20461 tccacgactg tattgccagg atggaattct cgagaagtca atttgtatgt gtttaaaata
    20521 cgcacagcat tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa acttcgcgta
    20581 tcacctttta gtccaatcac tactcgttct gttccttgat cgattgagac tttgaatcct
    20641 tctttttcga tatgatcgac gacttttttt atttgttctt ccgttgcttc ttttttcata
    20701 attactatca ttgtttttcc cgctttcttt tgtttactta tctatcttgg ctgattttct
    20761 ctactttttc aaggtggttt tacattaatt cctactaaat gaccgtgtcc tgttgttctc
    20821 attttcgctc cttctttcct acttatgacc cagataatca acaaaaaaag atgtccctgt
    20881 aggaaattgc ctctacagtg aacatcttca acgagcctta aaaagcgctg caaaaatata
    20941 aaatagccac tatagatgaa tttctctacg tggctgtaag tcgtgtttaa aaatctttag
    21001 ccatcataga tacaaacctg gtttgtttgt acccacgaag tcatgaatac aaacctatct
    21061 aaaatagcta aagtaatacg tattcagttt tgtaagtttt ctagatttca taaaaaatcg
    21121 ctccaaactg atgtaagaat tgtacctagt atagacataa aaaaatcgac tgtcaatcat
    21181 ttttatgaaa ctttttttaa ttttctgata gttaactttg tttttcagaa aaataacaat
    21241 tgattttatt gaagaaaata gtcatgaaca aaagtatctg agaacttttt tactctcttg
    21301 ttcatgacat atagaataaa gcagctttgt cttgtaaact ccgtttttca aataataatc
    21361 taaagattgc tactgaataa taaagccgat ttcatggtcc tcttcgctgc tgattcggta
    21421 aggtgactca acatccgctc cccagctatt gatacccccg actcctctta cttttgcaaa
    21481 aatcgtaaga accgttcgtc tttctggcgg tagttcttct atatgtgttg cctgttccag
    21541 ttcttctgct ttgaatggta aagcggaaaa agcaaatggc gtatcctgct gttcaaatcg
    21601 caacgaaaaa tcttcttgat ctttatccgt attatccaac gtttgatttc ttgttagttc
    21661 tacccatttc gtttccatat gcatgccgca ttcttgggga acaaggtatg gggaaacggg
    21721 gagtcctgcc actttataaa taccttcgac accaccagcc atccgatccg gatatgtttc
    21781 ccctgatagg ccttcatact caaaaaattt tgctttcgtt ggcataatga accttaaacc
    21841 caatgcgggt aattccggta atcctttttg tccataataa tgcatattga caagcatttt
    21901 accctctcca gcgatttggt aggaaattgt cacttgggtt tttggtactg tgttgatttc
    21961 cagtgtaaaa atgatttcaa ccgtttcagc gtattcatta cttgaatatt gattatttac
    22021 tggagcaatc ggcaaatcga tctttttatt gtccaccagt acttctattt ctttgacttt
    22081 tggatacaat ccggctccta accacatacc ggatttaaat ccaaatccgc tacctctgtc
    22141 attatcggtc gttgctcgcc aaaaaactgg aagtgtttcc cggtatagcc attcttttcc
    22201 gtttttattg agcgattcca gtccgcctct tgtataagaa aaaatataat gcgtattccc
    22261 cgatttgaca cctaatgtac agtcgccgaa gacgattgca actttttccg tatccatatg
    22321 acttttattt actgtatttt tcataataat atcttacctc ctgcatggcg ggtttttctt
    22381 gtccatctgc aaaaacgatc ccattccctg agaattcata gtcacttggt ttatcgtcaa
    22441 agtcaccgcc atagcgcatc acttctctgc ctgtgattgg atcagtcact tggatcgcct
    22501 gatctttaaa atcccatata tagcctcctt ggtacatttc ataacgatca agcaacgtca
    22561 tgtaagaatc cattccacct aacgaattcc ccatgtcatg catatattca cataagatga
    22621 acggtttttc cgctttattt tcaaggtagt caatgatctc ttctggtggc gcatacatgc
    22681 agctttttac atctgaaatc gaatcttcga atgctggatt tggaaaattc ccttcataat
    22741 ggacaagtcg atcggggtct acttttttga agtaatcgtt catttgtcga atgttttctt
    22801 ccgcataaga ttcatttcca agagaccaaa aaagaatact tggatggttt tttaatgttt
    22861 cgaaattcgt ttttgctcga tcaagaacag ccgcctgcca ctgaggtact gcacctggta
    22921 cgttgtaaga aggctcaaat gcgcccattt tttgccatga gccatgggat tcgaggttcg
    22981 tttccgccat cacatggatt cctgcttggt cgcataaata ataccaagtg atctggttgg
    23041 ggtaatgaga tgtccgtacg ccgttgatct ttgcattttt tatgatagcc aaatcttttt
    23101 ccatatcttc tatagtgatg gcgcgtcctt ttttcggatg ccactcatgt cgatttaccc
    23161 cgttgataat catccgtttt ccattgaagt agatgatttt atctttgatt tcgattttac
    23221 gaaaaccgaa ggaatagtca atatactccg ttactctttt ttcttcgtct atcgtctcca
    23281 attcaagctt atataaattg ggctgaaaat gcccccacag tgccacattt tcaaatccat
    23341 cgatgcgaat cgtcatttgc tcttttaacg gatattgctg ttgatagatt tccttcccat
    23401 caggatctag tatcgctaca ttcagctgac tgtttttggc tacttgctca aaagacaaat
    23461 ctacttcaag tttgccattt tgacatttgt catctaagcg aggtttgatc caaagatctt
    23521 ctaaatggtt ttgtcctcga gcaactaaga agacatctct gaagatccca aaaaatcgga
    23581 aaaagtcctg atcttccaag aatgcagctg tacttctttt gtaaacctcc acagctagaa
    23641 tattttcttc ttccaaataa ggtgttaaat caaattcaga cggagtgaac gaatcttcgg
    23701 catacccgat aaaatgacca ttcaaccaaa taaacattgc ttgttctaca ccagcaaaat
    23761 gaatcgagat ttgttttccc tttagacctt cttctaatcg aaaagtcgtc aaatacgaac
    23821 caaccggatt gtctttagca gctgaaaaga tcccttccgc ctctttctca gggcttgtcg
    23881 tgaaaggggg acggcgaaat acatgtccct cccacggata catcgtattg atgtaatgga
    23941 tcttgtcata atttgctaat tcaatatgac taggaacagt gatttcatca aaagaacagg
    24001 catcaaaatc ttttttataa aatgtttttg gtcgtaccga aggatttggc gaataacaaa
    24061 aacgccatga tccatttaaa gactgtttaa gggaggatgt ggtttctact tcttcccttg
    24121 ttgcataaca aacatgatcg ctatgtgcat ttgattggtt cactctaaaa acttgtggat
    24181 catctaacca ttttatattt gctttcatac ttttttcctt ctctctcata actttttgaa
    24241 atgaagcagt tctgtttgga aatcaccttg aaaatcgggg aagtaaagtc caactgtcat
    24301 caattcggcc cccgaaaata tccgcttcga ggtctgttcc tgataaagtg cctcatcatc
    24361 taaccccatc agatatactt catggaacgc tggttgagct gaagctagta tcctccctaa
    24421 aaacacaata gcttcatctt gtttaggaga cacaaacatc catgcggcaa tattttcttc
    24481 aaaaggactc gccaaacgat aataatggcc atactggatc accggacgaa tcgtttgata
    24541 ggatataatt tggtctttta cttgcttttt ttcttcagga gacagctttg tcaaatccag
    24601 ttcataacca aaaacagcgc tcatagctgt ttctcctctt gttttcaacg aagtagatct
    24661 gtcggtctga tgatttggta cagctgatac atgagctgtg atggacgaaa tcggataagc
    24721 aaggcttgtt ccatattgga ttttcatacg ctccactgca tccgtgttat cacttgtcca
    24781 tgactgcggc atataataaa taaacccggc atcaaaccgt ccacctccac cagaacagcc
    24841 ttcccataag atttccggat aatcagttgt caaatgttca agtagttcat aaagcccgag
    24901 catataacga tgcgctactt ctccttgtct ctgtgggtca agggtaatcg aatagacatc
    24961 tgaaagcgaa cggttcatat cccatttgat gtagtcaaga ggaatcgtat ctaaaatctt
    25021 tctcatttgt ttctcaatcg tctgccgaac atctaacctt gtaaaatcca gtacatgttg
    25081 tgatctggaa gcagaaggca ttctacctgg ctcctgcata agaaaatcag gatgtgttcg
    25141 atacagttca gaatcaaccg agatcatttc aggttcaaac cataaaccaa atttcaatcc
    25201 tttgctatga gcataatctg ctatttcacg taatccattt gtcaacttgc cttcatactc
    25261 gaaccagtct cctaatgaag aattgtccgc atctcttttt ccaaaccagc catcatctaa
    25321 tacgaacatt tcgatgccta gctcctttgc ttcatcaatg atcgccttga ttttttcact
    25381 attgaagtca aagtaagtgg cttcccagtt attgaccaga atcggccgtt cagcaaattg
    25441 atgacgacct cttgcgattc tttcacgtag caactcatgg tggatctgac tcattttgtt
    25501 caatccctga cttgagtaag aaaggatcat ttcaggtgtt tgaaaagact ggcctgcatt
    25561 caattcccac gaaaaacgat ggctgttgat cccaccgacg actctcaatt gattgatctg
    25621 atctttttca agttcgaaag aatggttgcc ggaataaaca aattgaagtc cgatcgcttc
    25681 tccagtattt tctgtcgtat gatgatgaac taaagcaata aagttattca tatggtgact
    25741 gctcgttcct cgacggcttt caaatacttt tgttccagat aaaacagatt gccgtgaaat
    25801 ctgtctttca ttgacatgtg ctcctggcaa agcaatgact tcatcaaatc gcctagtatg
    25861 ggcaaagtct agctggaaac tagccgcttt ttctagtttg attgtttctc cggtttcatt
    25921 tcttatcttt acagatcgtg tgatcactgc tctatcagta aatatggtat agaacaaatc
    25981 gaaataaatc gctgtttctc tatccttaag tgaaatcacc aacgtttgtg cctctgactt
    26041 gtccatcaca taagtttgag gcaagccttc gatctctggc ttcccatctg taatataatg
    26101 gctgtcatat ctcagatcca gtaagtctga tccattcttg cgctgaatga tcgatgcggg
    26161 cgttcgaaag tccatgctgc catgagaaga gtattcttgc ggtagtgtat ctttcgacag
    26221 agaccggtca ggatttaagg gaacatttcc tgaaaatcca cgatctcttc ggggataatt
    26281 tttattgtct ttgtactgct tgactggttt gccaaaataa atatgcgcca atacattgct
    26341 ttcttctatc tttatcagat aagaaagaaa tgtatttcgt aaatggaaga ctcggctctt
    26401 ttcatcaaat aagatgaaat ttttatctgt ttttctatgc gtaatatcca tcatttcact
    26461 cccaacaaaa cggaaaccgt ttactttttt ttataattcc tgtatactgt ttactaaaca
    26521 attttgtcaa taaaaatttt ttattttttt gaggagtgtg taaaaatggt tggaattaga
    26581 gatgtcgcac gaaaggcaaa tgtctctcct gctacagttt cccgtgtgtt gaataacgat
    26641 cagacgatcc atgtggctca gactactcgg caacggatag tagaggcagc tgctgcgttg
    26701 gattatgaca tcagtactcg taaatatatc aaaaagagaa tgccttcgat tggtgtgatc
    26761 tctacgatcg gcaaagcgag cgaagaacag gatatctatt acaaacattt gcgagtaggg
    26821 atggaagaag aagctcgacg tctgcattta gggatgaatc gaatctataa tctatctgat
    26881 aatcctaaac aatgggaaga tctagatcag ttaggtgcgg tgatcgttgt tgggactgtt
    26941 acgaaagaat cgattaccgc cttgttaaaa caaaacatgc aaatagtcgt cgtggataac
    27001 cctgacatcc agcaagaggt cgatatggtg tatgcagatc ttgaacggat gactaaaacc
    27061 gtactggaat tgttcttaga aaatggtcat aaaagaatag cctatatcgg gggatataaa
    27121 gtcgatatgg atgaattcgg tgggaagcag atttctataa atgaaaagag gatgcgtgcc
    27181 tatcagcatt ttatgatcga tcatcactta gaaaaatata tgtcttatca tctaggtggc
    27241 tggacagaaa aacaaggtga ggtacttgcg actaatctat tagaaaatga atcccctctt
    27301 cctactgcca ttcttgtcgg gagcgatcca atggcaattg gtgtctatcg ggcattgaaa
    27361 caaaaaaata tccaaattgg agaagagatt gttgtagcaa gctttgataa tatcgagaca
    27421 atcgcaaacc tctcccctgg cttgaccagt gttcaaatca atgcgaaatc aattgggcaa
    27481 gcagctgttc gaatggcatt ggagcgaatt gacggtatac gggacgaacc aatcattttg
    27541 acttatcctg cacgattgat tgttagggaa agttttatcc caagaacaaa aaggtattga
    27601 aagagaggtt gtgaaaagcc gcttcgtttc ttcatactca aaacgaaacg gctttttcac
    27661 aacctcttac ttatacttat tcacaaaatc caatgtctgc tggtaacaat tttgcgattc
    27721 ttttagcgtg taatcaaatt ggtattcatg ggtaatctct ttttttgtat ctgtataaaa
    27781 caaggattgc acgggtacct ttaattctgt caaccgattt tctaatgctt gaccttgctc
    27841 ctggaatgag tatgcatttc cgtcagttat atacgttggc ggaaattcct tagaaacatg
    27901 gtcagccaca ctagcttctt tcaattgctc actcgttttc caatcttttg ttccaatcaa
    27961 cgaccacgca acggttttga cgaaaaactt cataaatcga tcatccgaat gctgctgtgc
    28021 tgttagcttc aaatcaacag gtccgcaata agaaatggtt cctttcagac tatcttttgg
    28081 aagtagctgt ttgattttca tttgtttagc atacgcatca ttcgtttgta tagctgcata
    28141 ttgcaatgcg atttgtgaac ctgcactatc tccaccaaaa aataattgtt ccatgtctag
    28201 caatttgtcg tctttttctt cagctaataa atctttgatc agttcattca cttgaagaac
    28261 ttgattaggg tattctgaat caggcgctaa ttcataattc atcgcaacca ctgcgatgtt
    28321 ggcgtctgaa actaattttg tcgcaaattc tttgacaccc gatttatctc cgccgacgaa
    28381 tcctccacca tgtacccaaa atagaacagg aacaggcccc ttgctgtttt ttggatagta
    28441 gatgtcataa gtattctctt tatacttgga ctcatacgtc aaatctgttt ttttcgccac
    28501 gtttttttct gcttgatcga acgttttttt atcagtgatt tgaacagtat ttgaaaacat
    28561 atggttgatc aagaatgctc caggtctggg ggaaagctgg aaggcaagaa agattgcaaa
    28621 cgcgatccca agaatgattc cgaagaacca tccgataaat ttaagaattt tctttttcat
    28681 aaggctccct cagtagttag tattaagcat tctgattata ccatagtgtt atttcctatc
    28741 aagctctcct agatgattta atgattttcc ccaaacttct tctgcaatgc ttttgacaaa
    28801 agaaattttt tccattgttc ttcaaacaaa acattttctt cttctgttga agcaaatcca
    28861 cattgaggac ttaagcacaa acgatccaaa ggcagatatc gtgtcgcttc gtagatccgg
    28921 tcaatcactt gctggtgctc ttccatttcg ccatttttcg ttacagtcaa aatctaagac
    28981 aactaattta tcttcagaga ctttctctaa aagaacaaaa ctcccagcac gttcactatc
    29041 gtattctaaa taataggcac ggacattttc ttgagtaaaa taatgggtct gctacgtcgt
    29101 catcccccct gatgccgcca aaatcgaata ataatttccg cgtcaaacat gtatatttag
    29161 tgttgaatca tttgagaata tgaaaataaa aatctttttg atcaatcagt taatcaaatt
    29221 gaaataacga aaaatctgag acagaatgac gttcattctg acccagattt gattttgcac
    29281 aactcaacag tccttattgg caaatcgcca aaataatgta gactaactaa aaagactaag
    29341 aaacaataac aaaagttaac acttcttagt ctttctttag cacgtctatt tattaaagat
    29401 tttagggcct tttctcatgt ttttacgatc attcttaggt gtttccattt gtttggatcg
    29461 gcctccacct tgtttctttt tgatcaattc tttcattttt tctttttggt ccatttttat
    29521 tctcctttaa cgaatgaagc gactaattcg atatgatggg tttgcgggaa aagatctaca
    29581 ggctgaactt tcttcacggc ataacctgct tctgcaaata acttagcatc tcttgcaaaa
    29641 gttgccggat tacatgagat atagacgatt cgttctggtg acatttcgat tgctgattca
    29701 ataaaatgat gatctaatcc ttttcgtgga gggtcaacta cgatcacaga tggtttgatc
    29761 cctttttctt gccatttttt cataactttt tctgcttttc cgacttcata atgaacattt
    29821 tcgattttat tatttaaagc attgaactgt gcatcttcaa ttgcttctgg aataacttcc
    29881 atcccataca ccttatccac tctgtctgct aaagacaagc caatcgtacc tattcctgaa
    29941 taagcgtcga tcacaatgtc ttttttatct aactcggcaa actcgatggc tgtttggtac
    30001 agatactctg cttgttgagt atttacttga taaaatgact ttgctgagat tcgataggtt
    30061 tttcctaata gctgatcttc gatatagtct ctgccatata aaactttttc tttgtctcct
    30121 aaaatcacat ttgttttttc ttcattgata ttttggatga ccgaaaccac ttctggcaat
    30181 tcttcatgaa tagcttcagc aatttccttg gatttaaaga atttttcttt acgagtaact
    30241 aaaacgacca tcatttcatg ggaataatga ccacgacgga taatgatgtg acgaagctgt
    30301 ccttcatttt tttcttcatt atatcctcgt acttggaacc gttgcaagat atctctgact
    30361 actttgattg cttgatcgat gacaggatct tggatataaa aatcttcgat agggactaga
    30421 tcatggctat tctttttgta aaaaccggtg gctaactgtc catcgatacg gcgaacaggg
    30481 atctgtgctt tattacggta gccgaatggt tctgccattc cgaccgttgg aagtacagga
    30541 acttctggca tcttagcaat cttcgccatt acatgctcta cttgtttttg tttgaagaca
    30601 agctgttgtt catacttcaa atgggctaaa ggtgcgatac ctatgcgaag taatgcattg
    30661 ttttctacag gctgacgatc aggtgattgt gtaataaatg tttcgacttt tccatatcca
    30721 aacttattgc ctgcttttac tactagtatt tctacttctt ctccagggag tgcattttcg
    30781 ataaataatg gataaccatc gatttttgct acccccattc cttcataact caagtcgacg
    30841 atttcgactg tatatcgttc gtttttcttt actatttgct gttctgtcat attttgctcc
    30901 tttaagccta tttttcaatc ctactgaact ctactataaa tcagctaatt acacaactaa
    30961 aaccggcact gaccttacta cgttcagcac cggcttttga ttaattcttt tgattaggtg
    31021 tttcgtctgc tatttttccg tcaccgtcga tatcttcatc cgttaaacgt tcgacttctt
    31081 ttacgaattc tttgctgaca acctctaatt cttcttcatc tgtacctgtg attgcttcat
    31141 caggaatctg atcggtattg gcaaagaact caatgtgttg atgaagattt tggaaatgca
    31201 tcggtgcttc cccgccgtat tcaccatcta agttgatcat tactggtcga ttgatatcat
    31261 cttctgtttc aacgaacaaa ctgcttgttt tggtatagat tattcttgga tcatcgacat
    31321 gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat ctcaaagata tttgctgtct
    31381 tcacaatgat taaagaaaac ttcccatcat ccaatttggc atctggcgcg atctgttcga
    31441 atcctccaac agagttcgtc agccctagga aaaacatcga agcatttccg atatattccc
    31501 cttcatcata cttcaagcgc attttgattg gctttactcg agggagcatc tctgctcctt
    31561 tcgcaagata agcaagataa ccaaaaatac tctttagttc tgatggaact tcataagtca
    31621 attctgtcaa atagccacct gcagcgatat tgatgaagta actgttatca gaaaggccga
    31681 tgtccatttt tacggtttgg tgttttttta tcacttctgc tgctgcttta atattatctc
    31741 taggaatttt taatgctcga gcatagtcgt tcgttgtacc agctgggatg atggccattt
    31801 taggacggtg ttcaagcgga gcgattccat taaccacttc gttgattgtg ccatcgccac
    31861 cagctgccac tactagatca aaacctagtt ctgcgactct acatgcttca ttcttggctg
    31921 aatctggttc tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct tctaatgcct
    31981 gtaggatatc cgccaaattt tttttcaata attcttttcc cgaggtgggg ttataaatta
    32041 cgcgtgcttt tttcattttc agcctctatc gtttcgctaa ttcatcttta agtagcttat
    32101 tcacgacacc aggatttgct tgtcctttag ttgctttcat gatttgaccg actaggaagc
    32161 caaccgcacg atcttttcca tttttgaaat catcgacaga ttgttggttg ttatccaaaa
    32221 tttcattaat gattggcagt agtttgttcg gatcagataa ttgcacccaa ccattttttt
    32281 caactacttc ttgggcttct ccgccatttg tgatcaattc acggaagact tttttggcga
    32341 tttttgagct gatcgttcca tcggcgatca aacgaatcat tcctgctaag ttctcaggtg
    32401 tcaattttgt ctcagctaat tccagtttct cactgttcaa gtaagcagaa acttcaccca
    32461 tcaaccagtt agatgcttgc ttggcatctg caccattttc caaaacagct tcaaaaaaat
    32521 cagacatttc ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt ccaaactcac
    32581 ggatatagcg ttcgcgacga gaagctggca tctcaggcaa actagctttt actttttgaa
    32641 tccattcgtc atcgatcacg aatttcggga tatcaggctc tgggaagtaa cgataatcac
    32701 tagacccttc tttgacacgc atcaaaactg ttttattcgt tgtttcgtca aaacggcgtg
    32761 tttcttgttg gatttctcct cctgaaagaa gaacttttgc ttgacgtttt tcttcaaaag
    32821 ctaaaccttt tttcacgaaa gataaagagt tcaagttttt caattctgct ttcgtaccga
    32881 actcttcttg tccataaggg cgaagagaaa tattggcatc acagcgcata gagccttctt
    32941 ccattttcac atcgctgact tctgtaaatt ggataattga tcgcaatgct tctagataag
    33001 cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac gatttcgatc aaaggcgtgc
    33061 cttgtcggtt caaatcaacg taagaataac cgccaatacc atgcatgttt tttccagcat
    33121 cttcttctaa atggacacgt tcgatccgga tttttttctt tttaccttct acttcgattt
    33181 cgatccatcc gtcatgaccg attggttgat caaattggga aatttgatat gcttttggat
    33241 tatctgggta aaagtaattt ttccggtcaa aatgagtatc tttagagatt tcacagttta
    33301 atgccaaagc tgctttcatc ccgaattcca gcgcagcttt attcattact ggcagcacgc
    33361 ctggataacc ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca aaatgagctg
    33421 gcgctggaga gaaaattttt gagttggttt ttaactcgac gtggacttct agtccaatga
    33481 ctgtttcaaa attcattcgt ctttccctcc taaaatagct ggttgttgtt tatggaattc
    33541 tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa aataattacc
    33601 gatgatctgc aatccaactg gtaatccttc tgataaacct gccggtacag acatacctgg
    33661 aagtccagcc aagttgacag ggatcgttaa gatgtcactc atgtacattg tgattggatc
    33721 gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa gatggcccga tgatcaagtc
    33781 ataatctgca aagacttttt caaaatctcg tttgatcaat gtacggactt gtcctgcttt
    33841 tttgaagtaa gcgtcataat aacccgcgct taaggagaat gtacctaaca tgatacggcg
    33901 ttttacttct tcaccgaatc cttctgaacg tgagttgaca tatacatcat ctaatgattt
    33961 cacattttca gagcgataac cgtagcggat accatcgaat cgttgcaaat tggaacttgc
    34021 ttctgatgag gcaatgatat aataaacagc cacaccgtat tttgaatgag gcaagctgac
    34081 ttcttccaca acagcaccta atgaacggaa tgtttctgct gctttcaata cagcttcttt
    34141 tacgccaggg gccacacctt cgcctaagta ttctttaggt aaagcaatct tcattccttt
    34201 gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag aagtaccgtc
    34261 tttttcgtca taaccgctga tagctgtcaa tgctaatgca ttatctttca ccgtacgggt
    34321 cattggtccg atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac gtgagacacg
    34381 gccataagta ggtttcatcc ctacgatgcc gttaaaagct gctggttggc ggatacttcc
    34441 cccagtatca ctacctaaag aaactggaat ttgtccagct gcaactgctg ctgcagaacc
    34501 accagaagag ccaccaggta cttttgttgg gtcccaagca ttttttgttt tcttgaaata
    34561 agaagtttct gtacttccac ccatagcaaa ttcatccatg tttagcttac caacagggat
    34621 catatctgct tgatagattt tatccatgac cgttgcatca taaattggtt caaaattgta
    34681 aaggatctta gatgcagcgg ttgtcaagat atcttttgtt acgatattat ccttgatacc
    34741 aattggaata ccagcaagga cgttgctttc atcaatccct ttttcatcta aagcttttgc
    34801 ctgttccaat gctttttctt cgttcaatgt aatgaatgcc tcgacctcag gctctgtttc
    34861 tttgattcgt tcaaatgttg tttttgtcaa atcttgtaca gtgatttctt tcgatacaag
    34921 catatgatgc agttcttcta gagaacgatt gtgtaattct gtcattatgc gccagcctcc
    34981 ccattatcga tgattgcggg tactttgata aagccgtctt ctgtttccgg cacatttttc
    35041 aataattcat cgcggttcca tccagcttgc gcccgatctt gtcgcatcac gtttactgtt
    35101 tcaaccacat tcgacgtaaa tggaacacct tccgtatcta cttcttccag catttcaacc
    35161 atatcgatga ttttacctaa ttggtcggta aaacttgcaa gttcttcatc tgcgaacgca
    35221 agttttgcta atttggcaac gtgtttcact tgttcttcac taatcgccat actacccctt
    35281 ctttctaaac gagttaaaaa ctttgctctc tattttaaca caagagtgcc gtgtctgaaa
    35341 ttgtttattg aaatttgtct gaaaaaactc atttttcatt cttctactga gcgactttta
    35401 gccaatgcat ctgccatttg atcttctgtc caaacttcga ttcctaattc ttgtgctttg
    35461 gtcagcttgc tgccagcatc ttctcctgct accacgatat ccgttttttt cgatacgcta
    35521 ccagtcactt tcccgccaag attttcgatc gtctcttttg cttcttcacg agtaaatcgt
    35581 gttagtttac cagtaaggac tactgttttt tctttaaaag gagactcgac ttcttgcaat
    35641 tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa ttcattcatc
    35701 aattcatgga cttcttcgtt actgaaataa gtgactacac tgtcggcaat gatatcaccg
    35761 atcgtatcaa gcgcaataat cgactcatag tcacttttac tcaatgtttc tagatcaccg
    35821 aaatgttctg ctaaaatttt tgctgctttc gctccgacat gtcggattcc taacccgaaa
    35881 atcaaccgct cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat attatttgct
    35941 gatttttctt ttattttgtc caacgtcaat aactcttctt ctgtcaattt gtaaagatca
    36001 gcaacatctg ctactagttt tttatcatac atctgttcta gtacgcgcgg ccccaaccca
    36061 tcgatattca tcgcatttct tgaaacaaag tggttcaaac cttctttcat ttgagcaggg
    36121 catttaggat tgatgcaccg taaggctact tcttcatcca aatggacaag ttcacttcca
    36181 caaactggac aatgtgtagg caattggtat tcttcgctgt ctttaggtcg tttatctaac
    36241 acgacttgcg aaacttctgg gatgatgtct cccgctttat aaatcagaac tgtatctttc
    36301 aagcggatat ccttcaactt aatataatca ccgttatgca ggctggcacg gcttactgtc
    36361 gttcctgcta ctcggactgg tgtcatgata gctgttggcg ttactacccc tgttcgaccg
    36421 actgtccatt cgatgttttc gattagcgtt tcaacttctt ctggcggaaa cttgtaagct
    36481 gctgcccatc tcggtgcttt gatagtaaaa ccaagttgat cttgtagcga gaattcattc
    36541 actttgatga cgattccatc gatttcatag ggcaaatcca cacgtttgtc atgatattct
    36601 tcaatgtatg accacacttc atcaatcgaa tgacatagac gtttttcccg attcgtgtgg
    36661 aatccaattt tttctagctc ttctagcgca tcatactggg tttttgcttg cataggaccg
    36721 aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat tttagaatct
    36781 aactgacgca aagaaccagc tgcggcgttc cgaggattcg caaagatatc ttttccctct
    36841 gcttctctgt cttggttcaa ttggacaaat gaccgttttg gcatgaaaca ttcgcctctt
    36901 acttcgatgt tcattggttc ttttaatttg attgggaccg accggacagt ttttaggttt
    36961 tcagtgatgt tttctcctac agttccatcc ccgcgagtcg cacctctcac gaaatttcca
    37021 tcttcataac gcaaagaaac ggaaagtcca tctattttta gttcacagca atagtcaact
    37081 acacgtccca ctgctttttg tacacgttga tcaaaagcta tcagctcttc tttgctgaaa
    37141 acgtcgttca aactgtaaag cggaatatca tgagtgactt tttcaaaacc ttctaatact
    37201 tttccgccta ctcgttgagt gggagaatca gacgtgatca aatcaggata ttgttcctcg
    37261 attgctacta attctgcgta ttctttatca taaacatagt cttccactgt tggtttatct
    37321 tctacgtagt attcgcgaga ccattggttc agtcttgtac gaagttcttc tgctcgttta
    37381 gcagcttcgt caatcgtcat ttcatgttcc atcatttacc ctccactact aagctttttc
    37441 aattggcgca aaggcagcca aaagacgttt tactccttga cttgggaaag ccacatctag
    37501 ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt cctagacccc attttttgtg
    37561 tttgacttta tctccaggtt tccagctgtt attttcccct ccagtagcac ttttagaggg
    37621 aacaaccgct gtctctggct gtttatagct atatgctgtt tttttagcga atacaggcgt
    37681 ttttttaggc gtaggacgca tgccttcgat ttccagtaac tcctgatcga tttcttctac
    37741 aaaacgagaa ggtcggttat attgcgtccg tccatataat gttctggaga aagcatttgt
    37801 taaatacaac gcttcttctg ctcgcgtgat ccccacatat gcgagacggc gttcttcctc
    37861 cagttcgctt tcttccatca atgctcgaga taaaggaaaa accccctctt ctagaccaat
    37921 caggaataca actggaaatt ctaatccttt ggctgcatgc aaagtcatca atgtcacttg
    37981 ggaagcatct tcttctaaat tgtcaatgtc ggaaacaagt gccagatcat ttaagaagac
    38041 cgttagtttt tcttctggtg catcagcgtc ttcttcattt tgctgttcga attgtttgtc
    38101 gaattcttgt gtgaccgtta aaaattcctc caagttttcg atgcgtgctt gtgcttctaa
    38161 tgtattttgg atttttaagt cttctagata tccgctacga tccaaaacct ctttcgtcaa
    38221 ttctgtcact gtcaagtatg ggatcatctg agtgacttct tggatcatct ctccaaatgc
    38281 acctaattgc tgtcctgctt ttccaccgat atttgccaga tccacatttt gcgctgcttc
    38341 aagcagtggc cattcatgaa gagaagcaaa actgcgtagt ttttcgatag atcctggtcc
    38401 gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg attgaatctt gaggatttac
    38461 cagcacattc aaatacgcta aaatatcttt gatttcttta cgatcgtaga acttgtgccc
    38521 tccaaccatt ttataaggaa tatttgcttt caacagagtt tcttccatta cacgagattg
    38581 tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc cgatgattgc ttcgaatctc
    38641 ttcttgcatc cggtctacga taaaacgggt ttcgtctcgt tcattatctg cacggtagta
    38701 agtgatcttg tttccttctt tgttttctgt ccacaagttt tttggttttc gattgctatt
    38761 gttttcaatg acttggtttg ctgcgctaag gatgtttttc gtggagcgat agttttgttc
    38821 tagcaaaatt actgcagcat ctggataatc tttttcaaaa tccaagatgt tctgcatatc
    38881 tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc acgacgcaca ggttcctaaa
    38941 tctacctgcc aataaattga ctagcgtata ctgtgcgtga tttgtatctt ggtactcatc
    39001 tacatgaata taatggaatt tgttttgata atacgtgagt gaatcaggat gttcttcaaa
    39061 taatcgaatc gtattcatga tcaggtcgtc aaagtccata cattgattat attgaagctc
    39121 tttttggtaa gcagcataac attttgccgc aatctcttcg taataactgc cttgcatttt
    39181 tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga gagattgttc ctaatatact
    39241 tcgaggatca tattttttcg gatcaatgtt cagttctttt agtatccgtt tcatcaaggt
    39301 cagctgttca gaagaatcga tgattgtaaa gttccggtta tacccgataa aatcaacatc
    39361 tcttcttaaa atacgcacac acatcgagtg aaaagtcgat acccagacat cttcgcctcc
    39421 agatgcgaga atcgcattga cacgttcttt catttctttt gcagccttat tggtaaaagt
    39481 aatcgctagg atgttccaag gattgacttc tttttcctca atcaaatagg caatgcggtg
    39541 tgtcagcact ctcgtctttc cacttcctgc acctgccatt agcagtaatg gtccgtcagt
    39601 gtgaagaaca gcttctttct gacgggggtt catcccgttt aataattcag ctttggatga
    39661 cattaacaac atcctttctt ttatcaatct tgttaattat agcattttct tggctttcat
    39721 taaactagct agttcaaaat catttgtttc tttttatgga tttttccttg tcaattcgtt
    39781 gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag gaaaaatcaa
    39841 gaaattagat aagactcaga tatattttat tctaaaagga atatttgttg gactgctgac
    39901 aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt tagttcgaag
    39961 tatctatgaa atgagtcggg aacagccgat ttacttaatt ggcctagcta tttgctgcat
    40021 ccttgcagca tttttcgtgg gttatctggt caaaaatgaa ccggatatca agggaagcgg
    40081 gattccacaa gtagagggac agcttcgcgg ggaattatca atgaactggt tctctgtttt
    40141 atggaaaaag ttcattggcg gtgtactttc agttggtgcg ggcctttttc taggtagaga
    40201 aggaccttct atacagcttg gcgcatccat tggacaagga gctggacaat tgtttcggag
    40261 tccatcttca gaagaaaaga ttttaatttc tagcggtgca agtagcggac ttgcagcagc
    40321 attcaatgct cctatcgcag gattactctt tgttttagaa gaaatccatc actccttttc
    40381 tccacttgtt tggctgacat catttgcttc agcaatcact gctaacttcg tctctcttta
    40441 ctttttcggc ttgagacctg ttttatatct aggtgacctt ccttctttgc cgttgaaata
    40501 ttatggttcc ttagtgatct taggagtact tctaggcatc ttaggtttta tttatcagaa
    40561 tgtcttgctg gctttgccaa aattttacaa ccgattgcct ttaccagctt attttcacgg
    40621 gttcgttcct ttcttgctga ttttgccaat tggttattta tggcctagta ctctaggcgg
    40681 aggcaatcaa atcgttttag cttttggaca tgcttccttg ccaatcatta tgattatcgg
    40741 tttgtttgtt cttcgattcg ttttttccat gatttcttac ggtgggggtc ttccaggggg
    40801 gattttcctt ccgatattga cattgggggc tttaatcggt agcttttatg gaaacattat
    40861 ggttgatttc gggatggatc caatctacat caaggatttt gtcgttattg ccatggctgg
    40921 ttatttcaca gcgattggaa aagcaccgct gacagcaatt attttagtga cagaaatggt
    40981 tggctctctt aatcatctga tgcctttagg attggctgct cttgttgctt atatcgtcaa
    41041 tgaccttctc ggcggtaatc caatttacga atcattattg gaacgtctac ttggaaatac
    41101 tcagaccgcc tcactaaaag gtgcaaggac aacttttgat tttccagtaa cggcagagag
    41161 ttctttagac ggattaatgg tacgagactt caattggcct aaaaatatgc tgctaatctc
    41221 tatacgcaga ggaaatcagg aaatcttgac tcacggtgat acgatcatga atgtaggaga
    41281 tattttggtt atcttgactg atgaagcaaa cttgccaaga atcaagcagg aaatcgaggc
    41341 taaatcttct atcacaaatt tgaagcaatc attttaacga acatggaaaa actattttac
    41401 aacaactatt catctacact acttgattct atatgattct ataaaaaaag aagaggctgg
    41461 gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa
    41521 taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac
    41581 cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc
    41641 gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg
    41701 aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac
    41761 gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt
    41821 agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac
    41881 acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt
    41941 gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc
    42001 agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat
    42061 agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa
    42121 gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa
    42181 tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc
    42241 tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact
    42301 tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg
    42361 gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac
    42421 gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa
    42481 aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc
    42541 atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt
    42601 accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg
    42661 attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat
    42721 tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc
    42781 gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc
    42841 tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct
    42901 ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc
    42961 tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt
    43021 taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa
    43081 atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc
    43141 gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt
    43201 aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg
    43261 tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc
    43321 aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat
    43381 gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa
    43441 tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag
    43501 tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga
    43561 tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact
    43621 gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc
    43681 aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt
    43741 ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat
    43801 tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca
    43861 cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga
    43921 taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt
    43981 cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct
    44041 aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct
    44101 tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg
    44161 ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata
    44221 cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa
    44281 tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact
    44341 gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt
    44401 ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg
    44461 acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg
    44521 ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg
    44581 caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat
    44641 tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa
    44701 gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt
    44761 ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat
    44821 cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt
    44881 tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc
    44941 catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg
    45001 attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa
    45061 gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac
    45121 aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa
    45181 ttataaatca gtcaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
    45241 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
    45301 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
    45361 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
    45421 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
    45481 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
    45541 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
    45601 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
    45661 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
    45721 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
    45781 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
    45841 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
    45901 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg
    45961 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
    46021 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
    46081 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
    46141 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
    46201 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
    46261 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
    46321 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
    46381 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
    46441 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
    46501 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
    46561 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
    46621 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
    46681 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
    46741 gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
    46801 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
    46861 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
    46921 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
    46981 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
    47041 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
    47101 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
    47161 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
    47221 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
    47281 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
    47341 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
    47401 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
    47461 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
    47521 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
    47581 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
    47641 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
    47701 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
    47761 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
    47821 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
    47881 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
    47941 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
    48001 agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa
    48061 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
    48121 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
    48181 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
    48241 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
    48301 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
    48361 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
    48421 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
    48481 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
    48541 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
    48601 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
    48661 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
    48721 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
    48781 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
    48841 cgagcgtttt gcgcgatctt gtccaatcac gtagttatta aatacttcaa gaatttctag
    48901 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
    48961 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
    49021 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
    49081 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
    49141 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
    49201 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
    49261 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
    49321 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
    49381 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
    49441 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
    49501 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
    49561 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
    49621 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
    49681 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
    49741 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
    49801 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
    49861 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
    49921 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
    49981 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
    50041 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
    50101 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
    50161 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
    50221 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
    50281 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
    50341 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
    50401 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
    50461 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
    50521 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
    50581 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
    50641 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
    50701 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
    50761 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
    50821 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
    50881 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
    50941 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
    51001 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
    51061 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
    51121 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
    51181 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
    51241 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
    51301 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
    51361 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
    51421 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
    51481 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
    51541 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
    51601 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
    51661 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
    51721 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
    51781 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
    51841 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
    51901 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
    51961 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
    52021 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
    52081 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
    52141 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
    52201 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
    52261 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
    52321 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
    52381 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
    52441 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
    52501 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
    52561 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
    52621 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
    52681 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
    52741 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
    52801 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
    52861 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
    52921 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
    52981 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
    53041 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
    53101 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
    53161 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
    53221 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
    53281 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
    53341 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
    53401 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
    53461 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
    53521 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
    53581 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
    53641 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
    53701 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
    53761 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
    53821 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
    53881 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
    53941 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
    54001 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
    54061 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
    54121 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
    54181 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
    54241 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
    54301 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
    54361 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
    54421 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
    54481 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
    54541 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
    54601 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
    54661 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
    54721 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
    54781 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
    54841 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
    54901 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
    54961 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
    55021 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
    55081 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
    55141 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    55201 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    55261 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    55321 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    55381 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    55441 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    55501 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    55561 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    55621 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    55681 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    55741 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    55801 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    55861 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    55921 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    55981 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    56041 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    56101 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    56161 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    56221 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    56281 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    56341 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    56401 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    56461 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    56521 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    56581 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    56641 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    56701 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    56761 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    56821 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    56881 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    56941 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    57001 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    57061 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    57121 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    57181 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    57241 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    57301 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    57361 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    57421 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    57481 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    57541 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    57601 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    57661 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    57721 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    57781 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    57841 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    57901 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    57961 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    58021 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    58081 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    58141 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    58201 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    58261 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    58321 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    58381 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    58441 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    58501 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    58561 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    58621 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    58681 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    58741 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    58801 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    58861 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    58921 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    58981 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    59041 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    59101 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    59161 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    59221 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    59281 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    59341 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    59401 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    59461 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    59521 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    59581 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    59641 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    59701 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    59761 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    59821 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    59881 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    59941 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    60001 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    60061 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    60121 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    60181 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    60241 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    60301 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    60361 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    60421 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    60481 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    60541 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    60601 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    60661 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    60721 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    60781 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    60841 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    60901 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    60961 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    61021 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    61081 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    61141 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    61201 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    61261 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    61321 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    61381 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    61441 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    61501 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    61561 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    61621 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    61681 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    61741 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    61801 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    61861 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    61921 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    61981 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    62041 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    62101 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    62161 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    62221 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    62281 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    62341 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    62401 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    62461 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    62521 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    62581 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    62641 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    62701 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    62761 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    62821 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    62881 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    62941 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    63001 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    63061 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    63121 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    63181 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    63241 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    63301 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    63361 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    63421 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    63481 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    63541 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    63601 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    63661 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    63721 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    63781 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    63841 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    63901 gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    63961 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    64021 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    64081 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    64141 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataa
//
LOCUS       DADOOS010000003        60191 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.003, whole genome shotgun sequence.
ACCESSION   DADOOS010000003 DADOOS010000000
VERSION     DADOOS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 60191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.003"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="J8C37_000140"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="J8C37_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAZ5303608.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="J8C37_000141"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="J8C37_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAZ5303609.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="J8C37_000142"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="J8C37_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAZ5303610.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1640..2272
                     /locus_tag="J8C37_000143"
     CDS             1640..2272
                     /locus_tag="J8C37_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAZ5303611.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="J8C37_000144"
     CDS             complement(2274..3179)
                     /locus_tag="J8C37_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAZ5303612.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3917)
                     /locus_tag="J8C37_000145"
     CDS             complement(3264..3917)
                     /locus_tag="J8C37_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAZ5303613.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="J8C37_000146"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="J8C37_000146"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAZ5303614.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="J8C37_000147"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="J8C37_000147"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAZ5303615.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="J8C37_000148"
     CDS             6166..7251
                     /locus_tag="J8C37_000148"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAZ5303616.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="J8C37_000149"
     CDS             7248..8312
                     /locus_tag="J8C37_000149"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAZ5303617.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="J8C37_000150"
     CDS             complement(8561..9364)
                     /locus_tag="J8C37_000150"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAZ5303618.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="J8C37_000151"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="J8C37_000151"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAZ5303619.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="J8C37_000152"
     CDS             complement(10242..11249)
                     /locus_tag="J8C37_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAZ5303620.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="J8C37_000153"
     CDS             complement(11256..11900)
                     /locus_tag="J8C37_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAZ5303621.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="J8C37_000154"
     CDS             complement(12000..12758)
                     /locus_tag="J8C37_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAZ5303622.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="J8C37_000155"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="J8C37_000155"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAZ5303623.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="J8C37_000156"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="J8C37_000156"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAZ5303624.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="J8C37_000157"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="J8C37_000157"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAZ5303625.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="J8C37_000158"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="J8C37_000158"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAZ5303626.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="J8C37_000159"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="J8C37_000159"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAZ5303627.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="J8C37_000160"
     CDS             18156..19025
                     /locus_tag="J8C37_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ5303628.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="J8C37_000161"
     CDS             complement(19151..20050)
                     /locus_tag="J8C37_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ5303629.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="J8C37_000162"
     CDS             complement(20215..21039)
                     /locus_tag="J8C37_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ5303630.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="J8C37_000163"
     CDS             complement(21050..22345)
                     /locus_tag="J8C37_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAZ5303631.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="J8C37_000164"
     CDS             complement(22361..22840)
                     /locus_tag="J8C37_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAZ5303632.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="J8C37_000165"
     CDS             complement(22846..23874)
                     /locus_tag="J8C37_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAZ5303633.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="J8C37_000166"
     CDS             complement(23891..24997)
                     /locus_tag="J8C37_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAZ5303634.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="J8C37_000167"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="J8C37_000167"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAZ5303635.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="J8C37_000168"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="J8C37_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAZ5303636.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="J8C37_000169"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="J8C37_000169"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAZ5303637.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="J8C37_000170"
     CDS             29968..30666
                     /locus_tag="J8C37_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAZ5303638.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="J8C37_000171"
     CDS             complement(30834..33473)
                     /locus_tag="J8C37_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ5303639.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="J8C37_000172"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="J8C37_000172"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAZ5303640.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="J8C37_000173"
     CDS             35316..36491
                     /locus_tag="J8C37_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ5303641.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="J8C37_000174"
     CDS             36495..37136
                     /locus_tag="J8C37_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303642.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="J8C37_000175"
     CDS             37133..38050
                     /locus_tag="J8C37_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5303643.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="J8C37_000176"
     CDS             38047..38718
                     /locus_tag="J8C37_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303644.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="J8C37_000177"
     CDS             complement(39259..39747)
                     /locus_tag="J8C37_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAZ5303645.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="J8C37_000178"
     CDS             complement(39948..40214)
                     /locus_tag="J8C37_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAZ5303646.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="J8C37_000179"
     CDS             complement(40214..40477)
                     /locus_tag="J8C37_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ5303647.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40470..40742)
                     /locus_tag="J8C37_000180"
     CDS             complement(40470..40742)
                     /locus_tag="J8C37_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303648.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40953..41138
                     /locus_tag="J8C37_000181"
     CDS             40953..41138
                     /locus_tag="J8C37_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5303649.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41280..43103)
                     /locus_tag="J8C37_000182"
     CDS             complement(41280..43103)
                     /locus_tag="J8C37_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAZ5303650.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43701..46406)
                     /locus_tag="J8C37_000183"
     CDS             complement(43701..46406)
                     /locus_tag="J8C37_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAZ5303651.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46712..47659)
                     /locus_tag="J8C37_000184"
     CDS             complement(46712..47659)
                     /locus_tag="J8C37_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAZ5303652.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47849..48118
                     /gene="rpsN"
                     /locus_tag="J8C37_000185"
     CDS             47849..48118
                     /gene="rpsN"
                     /locus_tag="J8C37_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAZ5303653.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48294..48656)
                     /locus_tag="J8C37_000186"
     CDS             complement(48294..48656)
                     /locus_tag="J8C37_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAZ5303654.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48667..49788)
                     /locus_tag="J8C37_000187"
     CDS             complement(48667..49788)
                     /locus_tag="J8C37_000187"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAZ5303655.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49804..50154)
                     /locus_tag="J8C37_000188"
     CDS             complement(49804..50154)
                     /locus_tag="J8C37_000188"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAZ5303656.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50284..51795)
                     /locus_tag="J8C37_000189"
     CDS             complement(50284..51795)
                     /locus_tag="J8C37_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAZ5303657.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52117..53469)
                     /locus_tag="J8C37_000190"
     CDS             complement(52117..53469)
                     /locus_tag="J8C37_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAZ5303658.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53622..54698)
                     /locus_tag="J8C37_000191"
     CDS             complement(53622..54698)
                     /locus_tag="J8C37_000191"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAZ5303659.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54894..56639
                     /locus_tag="J8C37_000192"
     CDS             54894..56639
                     /locus_tag="J8C37_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAZ5303660.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56919..57356)
                     /locus_tag="J8C37_000193"
     CDS             complement(56919..57356)
                     /locus_tag="J8C37_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAZ5303661.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57340..58713)
                     /locus_tag="J8C37_000194"
     CDS             complement(57340..58713)
                     /locus_tag="J8C37_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303662.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59036..59524)
                     /locus_tag="J8C37_000195"
     CDS             complement(59036..59524)
                     /locus_tag="J8C37_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5303663.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59546..60148)
                     /locus_tag="J8C37_000196"
     CDS             complement(59546..60148)
                     /locus_tag="J8C37_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAZ5303664.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19082 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    43141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    43201 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    43261 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    43321 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    43381 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    43441 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    43501 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    43561 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    43621 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    43681 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    43741 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    43801 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    43861 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    43921 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    43981 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    44041 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    44101 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    44161 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    44221 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    44281 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    44341 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    44401 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    44461 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    44521 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    44581 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    44641 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    44701 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    44761 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    44821 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    44881 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    44941 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    45001 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    45061 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    45121 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    45181 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    45241 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    45301 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    45361 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    45421 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    45481 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    45541 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    45601 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    45661 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    45721 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    45781 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    45841 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    45901 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    45961 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    46021 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    46081 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    46141 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    46201 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    46261 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    46321 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    46381 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    46441 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    46501 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    46561 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    46621 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    46681 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    46741 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    46801 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    46861 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    46921 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    46981 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    47041 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    47101 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    47161 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    47221 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    47281 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    47341 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    47401 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    47461 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    47521 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    47581 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    47641 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    47701 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    47761 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    47821 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    47881 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    47941 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    48001 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    48061 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    48121 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    48181 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    48241 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    48301 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    48361 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    48421 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    48481 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    48541 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    48601 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    48661 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    48721 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    48781 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    48841 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    48901 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    48961 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    49021 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    49081 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    49141 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    49201 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    49261 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    49321 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    49381 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    49441 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    49501 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    49561 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    49621 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    49681 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    49741 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    49801 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    49861 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    49921 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    49981 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    50041 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    50101 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    50161 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    50221 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    50281 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    50341 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    50401 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    50461 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    50521 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    50581 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    50641 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    50701 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    50761 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    50821 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    50881 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    50941 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    51001 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    51061 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    51121 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    51181 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    51241 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    51301 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    51361 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    51421 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    51481 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    51541 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    51601 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    51661 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    51721 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    51781 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    51841 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    51901 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    51961 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    52021 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    52081 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    52141 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    52201 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    52261 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    52321 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    52381 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    52441 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    52501 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    52561 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    52621 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    52681 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    52741 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    52801 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    52861 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    52921 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    52981 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    53041 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    53101 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    53161 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    53221 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    53281 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    53341 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    53401 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    53461 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    53521 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    53581 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    53641 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    53701 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    53761 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    53821 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    53881 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    53941 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    54001 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    54061 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    54121 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    54181 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    54241 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    54301 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    54361 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    54421 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    54481 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    54541 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    54601 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    54661 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    54721 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    54781 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    54841 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    54901 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    54961 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    55021 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    55081 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    55141 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    55201 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    55261 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    55321 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    55381 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    55441 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    55501 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    55561 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    55621 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    55681 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    55741 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    55801 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    55861 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    55921 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    55981 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    56041 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    56101 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    56161 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    56221 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    56281 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    56341 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    56401 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    56461 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    56521 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    56581 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    56641 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    56701 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    56761 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    56821 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    56881 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    56941 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    57001 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    57061 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    57121 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    57181 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    57241 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    57301 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    57361 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    57421 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    57481 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    57541 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    57601 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    57661 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    57721 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    57781 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    57841 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    57901 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    57961 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    58021 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    58081 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    58141 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    58201 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    58261 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    58321 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    58381 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    58441 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    58501 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    58561 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    58621 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    58681 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    58741 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    58801 tcttgtaaaa aaaaaataca gaaagaagca taagcttctt tctgcatttt tacggtactt
    58861 gattactcaa gcagacaagc ccttctccac aaaagtcttg cccgacaccc ctgcatccgg
    58921 caggtgataa agatagaaat caattatcct gtgcctccaa aatcaattag gagctctgta
    58981 aaccagagtc cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg
    59041 cgagagcttt tttcatttta taatcgacca tttgaaatcc ttgaccgata tctgaaataa
    59101 gttcttctgt aatctcaaat ccttcatgct tataaacctc gattgcttga tgattcccct
    59161 gattcactcg caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc
    59221 gaaaaactga agaagtcaat ccttgaccac gtactttatt tagcaaatat aatttactta
    59281 aataaatttt tccatttatc tcttcataag cagtgtaacc gacagtcttt tcttcgcttt
    59341 ttaataaaaa gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat
    59401 aagttttcag catgtaatcc acttgttttt gaccaatgat cggcacaaaa acttccggcc
    59461 atacttcttg tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt
    59521 ccatacaccc acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga
    59581 gcatgatcat gctatacaca cccaccgacc acggtttcaa gaaaatcaca atctgcataa
    59641 attccctaaa agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa
    59701 agggaagcag caaggtaaca actagtactt ttttaatatc tttcgttttt cttgtcagca
    59761 attcatcaaa tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat
    59821 agtaacggac aagcaaaaat ccgattgctt ccccaatcac taggccaata caactatata
    59881 aaagcccaaa tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca
    59941 aaataggaat gatcacttgg atcacttgca acagaacaaa tatcatcact gcatacttgc
    60001 caaattgctg gataaacgct tgcaaagacg tgctcgatcg aaaaatccca tgatgataag
    60061 cataaagaat caacacaaag aaaaaaaaca gaccaataat gggcatgatc tgaagacatt
    60121 tttgtaaaac tgatcctttt ttagacatct atattcccca catctttttt catttattaa
    60181 aactgcctcc t
//
LOCUS       DADOOS010000004        53273 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.004, whole genome shotgun sequence.
ACCESSION   DADOOS010000004 DADOOS010000000
VERSION     DADOOS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 53273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.004"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(524..1078)
                     /locus_tag="J8C37_000197"
     CDS             complement(524..1078)
                     /locus_tag="J8C37_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAZ5303665.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1204..3132)
                     /locus_tag="J8C37_000198"
     CDS             complement(1204..3132)
                     /locus_tag="J8C37_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAZ5303666.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3438..4154)
                     /locus_tag="J8C37_000199"
     CDS             complement(3438..4154)
                     /locus_tag="J8C37_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAZ5303667.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="J8C37_000200"
     CDS             complement(4155..5978)
                     /gene="glpO"
                     /locus_tag="J8C37_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAZ5303668.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="J8C37_000201"
     CDS             complement(5992..7488)
                     /gene="glpK"
                     /locus_tag="J8C37_000201"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAZ5303669.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7624..9084)
                     /locus_tag="J8C37_000202"
     CDS             complement(7624..9084)
                     /locus_tag="J8C37_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5303670.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9074..10369)
                     /locus_tag="J8C37_000203"
     CDS             complement(9074..10369)
                     /locus_tag="J8C37_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ5303671.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10701..11378)
                     /locus_tag="J8C37_000204"
     CDS             complement(10701..11378)
                     /locus_tag="J8C37_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAZ5303672.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11408..12499)
                     /locus_tag="J8C37_000205"
     CDS             complement(11408..12499)
                     /locus_tag="J8C37_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAZ5303673.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12600..13034)
                     /locus_tag="J8C37_000206"
     CDS             complement(12600..13034)
                     /locus_tag="J8C37_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5303674.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="J8C37_000207"
     CDS             complement(13618..15075)
                     /gene="ascB"
                     /locus_tag="J8C37_000207"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAZ5303675.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15072..16967)
                     /locus_tag="J8C37_000208"
     CDS             complement(15072..16967)
                     /locus_tag="J8C37_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5303676.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17025..18800)
                     /locus_tag="J8C37_000209"
     CDS             complement(17025..18800)
                     /locus_tag="J8C37_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ5303677.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18850..20346)
                     /locus_tag="J8C37_000210"
     CDS             complement(18850..20346)
                     /locus_tag="J8C37_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5303678.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20540..22162)
                     /locus_tag="J8C37_000211"
     CDS             complement(20540..22162)
                     /locus_tag="J8C37_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAZ5303679.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22611..23924)
                     /locus_tag="J8C37_000212"
     CDS             complement(22611..23924)
                     /locus_tag="J8C37_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAZ5303680.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24169..24393)
                     /locus_tag="J8C37_000213"
     CDS             complement(24169..24393)
                     /locus_tag="J8C37_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303681.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24413..24781)
                     /locus_tag="J8C37_000214"
     CDS             complement(24413..24781)
                     /locus_tag="J8C37_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAZ5303682.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24979..25689)
                     /locus_tag="J8C37_000215"
     CDS             complement(24979..25689)
                     /locus_tag="J8C37_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303683.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25707..26204)
                     /locus_tag="J8C37_000216"
     CDS             complement(25707..26204)
                     /locus_tag="J8C37_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5303684.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26241..26693)
                     /locus_tag="J8C37_000217"
     CDS             complement(26241..26693)
                     /locus_tag="J8C37_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5303685.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26828..27433)
                     /locus_tag="J8C37_000218"
     CDS             complement(26828..27433)
                     /locus_tag="J8C37_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAZ5303686.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27883..28503)
                     /locus_tag="J8C37_000219"
     CDS             complement(27883..28503)
                     /locus_tag="J8C37_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5303687.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28505..29374)
                     /locus_tag="J8C37_000220"
     CDS             complement(28505..29374)
                     /locus_tag="J8C37_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAZ5303688.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29397..29804)
                     /locus_tag="J8C37_000221"
     CDS             complement(29397..29804)
                     /locus_tag="J8C37_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAZ5303689.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29823..31112)
                     /locus_tag="J8C37_000222"
     CDS             complement(29823..31112)
                     /locus_tag="J8C37_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5303690.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(31139..31441)
                     /locus_tag="J8C37_000223"
     CDS             complement(31139..31441)
                     /locus_tag="J8C37_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5303691.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31466..33559)
                     /locus_tag="J8C37_000224"
     CDS             complement(31466..33559)
                     /locus_tag="J8C37_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ5303692.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(34048..34812)
                     /locus_tag="J8C37_000225"
     CDS             complement(34048..34812)
                     /locus_tag="J8C37_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAZ5303693.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34923..35792)
                     /gene="mdcB"
                     /locus_tag="J8C37_000226"
     CDS             complement(34923..35792)
                     /gene="mdcB"
                     /locus_tag="J8C37_000226"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAZ5303694.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35817..36893)
                     /locus_tag="J8C37_000227"
     CDS             complement(35817..36893)
                     /locus_tag="J8C37_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAZ5303695.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36917..37510)
                     /locus_tag="J8C37_000228"
     CDS             complement(36917..37510)
                     /locus_tag="J8C37_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAZ5303696.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37514..39190)
                     /gene="mdcD"
                     /locus_tag="J8C37_000229"
     CDS             complement(37514..39190)
                     /gene="mdcD"
                     /locus_tag="J8C37_000229"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAZ5303697.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(39180..39479)
                     /locus_tag="J8C37_000230"
     CDS             complement(39180..39479)
                     /locus_tag="J8C37_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAZ5303698.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39581..41227)
                     /gene="mdcA"
                     /locus_tag="J8C37_000231"
     CDS             complement(39581..41227)
                     /gene="mdcA"
                     /locus_tag="J8C37_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAZ5303699.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(41234..42145)
                     /locus_tag="J8C37_000232"
     CDS             complement(41234..42145)
                     /locus_tag="J8C37_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAZ5303700.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42265..43122
                     /locus_tag="J8C37_000233"
     CDS             42265..43122
                     /locus_tag="J8C37_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ5303701.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43309..43671)
                     /locus_tag="J8C37_000234"
     CDS             complement(43309..43671)
                     /locus_tag="J8C37_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303702.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(43860..44042)
                     /locus_tag="J8C37_000235"
     CDS             complement(43860..44042)
                     /locus_tag="J8C37_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303703.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            44017..45456
                     /locus_tag="J8C37_000236"
     CDS             44017..45456
                     /locus_tag="J8C37_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ5303704.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45463..48015)
                     /locus_tag="J8C37_000237"
     CDS             complement(45463..48015)
                     /locus_tag="J8C37_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAZ5303705.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(48129..49460)
                     /locus_tag="J8C37_000238"
     CDS             complement(48129..49460)
                     /locus_tag="J8C37_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5303706.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49426..50904)
                     /locus_tag="J8C37_000239"
     CDS             complement(49426..50904)
                     /locus_tag="J8C37_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5303707.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(51209..53044)
                     /gene="tdc"
                     /locus_tag="J8C37_000240"
     CDS             complement(51209..53044)
                     /gene="tdc"
                     /locus_tag="J8C37_000240"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAZ5303708.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        15313 a        11450 c         8945 g        17565 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
      181 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
      241 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
      301 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
      361 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
      421 aacaacttct cctgtttttg gtttggttgg cttattatcg agaagtagct actgggagtc
      481 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
      541 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
      601 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
      661 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
      721 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
      781 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
      841 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
      901 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
      961 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
     1021 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
     1081 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
     1141 caatgatttc actgttttta tttttcttta cagccactga tattatgagc taatttgaga
     1201 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
     1261 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
     1321 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
     1381 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
     1441 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
     1501 tatccgatca ccttttgcaa agagctggcg ttctttttta ttctccttcc tatttgtaac
     1561 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
     1621 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
     1681 gctttctcct gtttctccaa cccgcaactg atatttagga cgaagtgctt cccgatcgaa
     1741 agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga tttctccata
     1801 atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga ttgcgatcgc
     1861 tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa gtaccaacgt
     1921 attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag aaaatgtact
     1981 caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat caaagacagc
     2041 aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca aagtcaataa
     2101 accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga taatcaagcc
     2161 tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta aaaatggatg
     2221 tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc ttccattgat
     2281 ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg tttcgatcaa
     2341 taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca ggacttgata
     2401 ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt tagaaatcac
     2461 agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg ttccatctat
     2521 tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt gaacaatcat
     2581 tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta attttgactg
     2641 gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc
     2701 gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat ttatcaaatc
     2761 aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct tatcaaaaaa
     2821 ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat cggttggctg
     2881 cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca accccataaa
     2941 tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc
     3001 caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt tgctatagtt
     3061 tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt gactaatcat
     3121 ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat aaataagggc
     3181 ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat ccttattaga
     3241 atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa taaactttgt
     3301 ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca taccccattc
     3361 ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt tgtcaaactt
     3421 tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat aattgctccg
     3481 atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc tttattagct
     3541 attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt gattgcatat
     3601 cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac agcgaatacg
     3661 ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac taatacaaat
     3721 gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg acctgttgca
     3781 aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa ataagaaagc
     3841 caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa aggaacgaca
     3901 agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg attcaaatga
     3961 gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc ccaaccaaat
     4021 gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac agccgcacac
     4081 acaccatctc caagcaaaac taggatcagt gtcccaataa actctcccat gatttgagtc
     4141 atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc tgcaatcaat
     4201 gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa atatccagca
     4261 attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa caacagatga
     4321 tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc taaaccataa
     4381 cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc ttttgctaat
     4441 tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc atcttcttcc
     4501 tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc taatttagtt
     4561 ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt cgtctctatt
     4621 ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc catcttacgg
     4681 taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct ttctaaagaa
     4741 ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa atggttcaac
     4801 acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac tgtatcgaca
     4861 actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta atcagaacca
     4921 gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc attcaatgtg
     4981 atatttgctt ctggatagcg atgattgatc acatccaata gataatctac atcttcttgt
     5041 gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc aaagtaagtc
     5101 ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt atcgaagtat
     5161 gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc tttagttgga
     5221 cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc tacccaagga
     5281 ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt cagcaggtca
     5341 cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc tttcatttta
     5401 cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac taaacgggca
     5461 tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt tttcaaaaac
     5521 ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt ggttcctgtc
     5581 acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat attgaaggtt
     5641 gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg gatgtgtggg
     5701 gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc cacatcaaat
     5761 gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga agtcccttct
     5821 gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga ctggactgcc
     5881 acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc ttgctgcatt
     5941 ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc cttattcaaa
     6001 tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc ggcgctcttc
     6061 ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct cgtctgtatc
     6121 tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa
     6181 gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca tcagatattc
     6241 attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag tgtcatcttg
     6301 catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag ctttaatgat
     6361 atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca
     6421 ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt cgcttgtaga
     6481 gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc catctctcag
     6541 ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt aaacctttcc
     6601 atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg gtttttcacc
     6661 ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc ctggttcgaa
     6721 tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc
     6781 gcttccataa aaatgataac cttgcgtata accataaact tctgagttag aagtgacttt
     6841 tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc aatctagctg
     6901 atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat gtgcttgtcc
     6961 atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt cgcctttttc
     7021 agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg ttgctgagaa
     7081 ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc cttcattttt
     7141 taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat gatagattgg
     7201 acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta tgccaatccc
     7261 agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa ctgactgtac
     7321 agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga aatattgcgt
     7381 gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga
     7441 gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg tgattccctc
     7501 cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta aaaaaagaaa
     7561 ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga agctattttt
     7621 taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta
     7681 aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct ggcaaaatgt
     7741 gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac cgccgattcc
     7801 cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca gactttctaa
     7861 atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa gaacattggc
     7921 atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg gcaattgctg
     7981 caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga gcattttctg
     8041 ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa agaaaaacca
     8101 ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga ttttcaaacg
     8161 acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg
     8221 taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa
     8281 agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg ctgctgttcg
     8341 actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg actgatagag
     8401 taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc tttttttcgt
     8461 aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac
     8521 acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg
     8581 ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct gtagctcttc
     8641 tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct ggttcaattc
     8701 tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta atcgaacgat
     8761 ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc tttcctgcaa
     8821 taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca gcgcctgata
     8881 ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt caatatcttc
     8941 caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac cattgataga
     9001 ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact gctcttttct
     9061 ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac accatatctg
     9121 gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca aacgtccatc
     9181 cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt ttgctttgga
     9241 gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta actttgcttt
     9301 gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg ggataaaaaa
     9361 tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac tgattgccga
     9421 ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg ctgacatttc
     9481 gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt gttggacgaa
     9541 aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt
     9601 catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac atctgattgt
     9661 cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt ttttccgtct
     9721 ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa
     9781 gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct agataacgaa
     9841 aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat gccaaccctt
     9901 cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt gcattcatga
     9961 tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata agcgttgatt
    10021 ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat
    10081 ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt actgtctttt
    10141 ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc caagaaagtt
    10201 gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga atccatccta
    10261 acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg cgaagataaa
    10321 tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc tgcattcctc
    10381 tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat tcataactcg
    10441 ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc ataccgtgtt
    10501 ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca tttctgaaga
    10561 gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg tacctctgtc
    10621 caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg taatagttag
    10681 aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc gcctaattga
    10741 tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac cacttctgaa
    10801 aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc tgctaagaat
    10861 ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc agtcactgta
    10921 cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc tacaacaccg
    10981 tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac tttcccaaga
    11041 tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata aaccgctgca
    11101 taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg atttctgaaa
    11161 tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact ttgcggttct
    11221 aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt ttttactaat
    11281 acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc gatcttttcg
    11341 tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc tttctataaa
    11401 aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc aaataaagct
    11461 ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt tccaataatt
    11521 tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt tctgcatatt
    11581 tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact aaagggatct
    11641 ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat ttatcagaac
    11701 gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt gactcacgta
    11761 atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca tattcttctg
    11821 gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc gctgagaaaa
    11881 ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg ccccattgat
    11941 cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta tttgctccat
    12001 gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg ccaaatgctt
    12061 ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt
    12121 ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct cgacttgtca
    12181 ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg attgctttcg
    12241 ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca cctggccagc
    12301 cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga cctttagaac
    12361 gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg
    12421 tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt aaagtaattg
    12481 attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa cctatcgaga
    12541 atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct taaaaaattt
    12601 tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc tttacttgct
    12661 tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc atcgacttcc
    12721 ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc ttcgctaact
    12781 gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg
    12841 gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt taatatcaat
    12901 aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc agctaattta
    12961 ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa cgctttttca
    13021 attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga tttttatttt
    13081 ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa aaagaagtag
    13141 ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca ccgtgaatca
    13201 gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga aaatggcttt
    13261 ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac accgtttata
    13321 tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa caaaaatagt
    13381 tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt acaccgttta
    13441 ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt caaaaaacag
    13501 cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga gaatactgtt
    13561 tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt ccttttatta
    13621 taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa
    13681 ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc cataacgttt
    13741 tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg tataaccaaa
    13801 taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt tcaaatagtc
    13861 aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag ctccgagtcc
    13921 gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca aagcaattct
    13981 caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg gatttttaat
    14041 tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag cactgactgc
    14101 actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta agatttcttc
    14161 atcacctact tctattttta agttgacacc tttttcttca aacattcgtt gcgtatacgc
    14221 tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt gctgctcaat
    14281 tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat agctgatcat
    14341 acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta aagcacttgc
    14401 tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat ggttttcttc
    14461 aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat tgaaagtcat
    14521 ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa
    14581 ctgaataagc tttcgattag gccatccacc atattctgta accaaatata acggcatttc
    14641 ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt caaacacttg
    14701 atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag gaaaaattct
    14761 tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata aacggatgtc
    14821 ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat acttgctgtt
    14881 gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat ctgtccattt
    14941 ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc ctttttcatc
    15001 tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata aaaatccttc
    15061 tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg attatttttc
    15121 ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac cattgtggtc
    15181 ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa atcgccttta
    15241 cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa attcactgta
    15301 tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc aattgcatgt
    15361 ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt tccgtccgtt
    15421 ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc gtttacttca
    15481 gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt tgaggattct
    15541 tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact gatcatcgct
    15601 aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa
    15661 atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac tccaccaaat
    15721 atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt cacaccataa
    15781 acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga taaacttttt
    15841 actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg cgcagctacg
    15901 gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt gttggtcatt
    15961 actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa tccgccgata
    16021 acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc tgcaatcagc
    16081 tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac catcacgatg
    16141 atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt gcgaatctta
    16201 ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt acttgtatac
    16261 cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact atatgcactg
    16321 acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg atctgtattg
    16381 aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct
    16441 gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa actcgtacat
    16501 aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt cggggcaaaa
    16561 acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt tcctctcttt
    16621 ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata cacttcattt
    16681 actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt gatcacacct
    16741 tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt aaagcgtaac
    16801 cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa tcggacaata
    16861 tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa tgccgtagca
    16921 atccttctta caagataagt atagttctta gaaaacggtt tctccattca attagtaccc
    16981 cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc gagcaaatca
    17041 ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga gacaaaaaaa
    17101 acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata ctctgtgatt
    17161 tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt taacggaagc
    17221 ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt tttatggatt
    17281 tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt tttttcttca
    17341 actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa ctcatctaga
    17401 acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc agtagtcaaa
    17461 attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac tggttctatc
    17521 cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag tgtattcaaa
    17581 atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa aggattcaac
    17641 gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg taattgaaaa
    17701 tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga gatgatcttt
    17761 tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat ttgtttgata
    17821 tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc taaaaaacga
    17881 ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt ttcatcatta
    17941 atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc ttgattggaa
    18001 aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat cggaataaat
    18061 aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt caaaacgaga
    18121 taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga tataagaacc
    18181 tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact gatttgttga
    18241 ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa aattcttttt
    18301 gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag ttctaattta
    18361 ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc tgtttttgaa
    18421 agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa caataagtac
    18481 gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt cgtcacctct
    18541 tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc tgaacgttta
    18601 tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat ttcagtttgg
    18661 atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat ccaatgattc
    18721 ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat ttgcttacac
    18781 ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc acttgcagag
    18841 gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag actggataat
    18901 ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc tcccatcatt
    18961 ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc ctgccgatac
    19021 gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt ttattgcctc
    19081 agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt ggattcgatt
    19141 caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata atggtaattc
    19201 ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa ttggccaact
    19261 ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg ctccgccagt
    19321 atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac taaatgcaat
    19381 ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta ctggtgcaca
    19441 gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc acatgacatc
    19501 ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct ctggatcaca
    19561 gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct tagcatttgg
    19621 atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat gatgacaggc
    19681 ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc tagcagggaa
    19741 tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct cattaaaggt
    19801 catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa
    19861 aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg ctaagggggt
    19921 ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg tattgattag
    19981 tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc ttggaaatat
    20041 tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca gtaaacgaat
    20101 atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat ggagtgattc
    20161 gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag gatgctggtt
    20221 catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca accatgcacc
    20281 ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa atatttcctg
    20341 atccataact atctctcctc gctatcaaca atttcttact tgaagtatag ctcgattcat
    20401 caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa tgctactctt
    20461 catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa aacactcgtt
    20521 aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa ggtcgtaaat
    20581 caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc cttaaagctt
    20641 cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg agccattgct
    20701 ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc actaattcat
    20761 agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc ttgtacatat
    20821 aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta taatttggat
    20881 tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg ttccattgca
    20941 taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt tcctcttttg
    21001 taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct tttgcttccg
    21061 cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag atataagggg
    21121 tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt cgatacacat
    21181 cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa aataaactat
    21241 tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc agttcagaga
    21301 tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat cctatgtgtt
    21361 cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt ttagcgattt
    21421 cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca aaagttgctt
    21481 gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt tctttatccg
    21541 gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct ttatcgatcc
    21601 agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa ttcaaatcag
    21661 gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc aactgccaag
    21721 cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc ggatcacgcc
    21781 aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg aaccacggat
    21841 gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt cttttctttg
    21901 cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg attgcttgat
    21961 aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga gaaagccaaa
    22021 ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc cgcaaatctc
    22081 caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa acaactgaat
    22141 ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct attttttgct
    22201 taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata gctattgcac
    22261 cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct caactgtttt
    22321 ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt ataaacggtg
    22381 ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg aactattttt
    22441 tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt tataaacggt
    22501 gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag aaactatttt
    22561 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    22621 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    22681 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    22741 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    22801 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    22861 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    22921 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    22981 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    23041 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    23101 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    23161 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    23221 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    23281 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ctatcccgat gaagatgcca
    23341 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    23401 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    23461 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    23521 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    23581 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    23641 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    23701 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    23761 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    23821 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    23881 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    23941 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    24001 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    24061 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    24121 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    24181 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    24241 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    24301 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    24361 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    24421 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    24481 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    24541 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    24601 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    24661 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    24721 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    24781 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    24841 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    24901 aggattgctt tcctccacaa acttttttta caaaaaaaaa aaacggcaat gaatgccgct
    24961 cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta gtgtgtgtct
    25021 aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga ttgcctgtgc
    25081 gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg cttgtgtttc
    25141 ttccgtcagc attgttcttt gggcttcacg ccagttgaga cagcgacaga tcgctccttg
    25201 ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg cacctaaagg
    25261 caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga tcttttcgat
    25321 atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga tatctacaag
    25381 cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag cttcaataga
    25441 agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact gagcaatgac
    25501 ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg ctgcctcatt
    25561 caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga taccatttac
    25621 ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat ccactattac
    25681 ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag atagtctgtt
    25741 tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc ttttttacga
    25801 taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc tgttttttgc
    25861 ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc ttgatgttta
    25921 aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat ttcctcagat
    25981 tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat actcactagg
    26041 aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc ataagcttgt
    26101 gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc tgtaaatgtt
    26161 tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg aatttcgtat
    26221 ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac aaaattccaa
    26281 tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt tgcttcttcg
    26341 tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat gatgaaagga
    26401 tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt ttctttatct
    26461 tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc agtcgttgta
    26521 cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt ttcgcaaata
    26581 cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc gatattgcta
    26641 atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc catttcatcc
    26701 cacctttatt ttgtttgtaa gactagttta cctctttttg ttgcaaatgc aactattttc
    26761 aataaaaaaa tcaagttctt ttttaataac tagtaaagta tattcttttc ttcttttacg
    26821 aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac tgtttcttcg
    26881 ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc atgttggaag
    26941 tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca aaaacaagtt
    27001 tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa actgctcgca
    27061 actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc acattttctt
    27121 caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc gtccgtttat
    27181 cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct acttttggtg
    27241 ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt tcttttccta
    27301 tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct ttatcaaata
    27361 catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg tattgagcca
    27421 acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt aatcaagtat
    27481 agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa taaaagaatg
    27541 ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata aatggcgttc
    27601 aaaagcttgc atctgcggta ataagcccca ccaaacgaaa aatatgacga gctattcttg
    27661 tctggctgtt cttattaatt gaggcaggac agctttttca caacctcccc actaaacggt
    27721 gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga gaagctgttt
    27781 ttttgaaatt gctccttgtg actcggagct gaacgacagg ctcacgacct ctcggtaaac
    27841 agcgttcaaa agccaatttt tcgatattaa gccatttctt tgttacttat tgtcattgat
    27901 ttctttttct aatttatcca atagtggagt gtaatccact tgagtatagt ctgttacatc
    27961 aaattcatat agtgttccta actggaattt gtcatatttt cggtcggcga tgatcttttt
    28021 gaatgcttct ttcgtaatat gattggtccc tttcgttcga tcttccaata cgtcaccata
    28081 gtgataatga tccatgatat agctcaaatg acgatctgat tcacgatcac gtttgtaccg
    28141 acgttgacat aacacttcaa agtctgctgt taggcgaatc gtgatcacct gatatccgta
    28201 tctatctgct aattcttgga gtcgtgcttt ttgcttatca ctaaaaggat actcggacac
    28261 aatcactttc tttcctgctt ccatatacaa atcaagtgct ttgtaataga aaggataaac
    28321 tttcgtcctt tcttgttccg cccgttcttc caaattgtta aacccgatgg attctgcata
    28381 gtattccttg atttcatcga gcgtgatggc atacatatct ggaaatcgtt cccgtagttt
    28441 attcataaga tatgtttttc ctgtaccagg actaccagcc agaagaatga aatacttttt
    28501 catctcattt atttcctaag ttaattggac tttccaagtt cataaacagt ccaatcttat
    28561 gcttgacaat ttcttttact gcttcatttg ctggaatcac attatggcga agagacttgt
    28621 tgatttctgt ttcactaaag gtttctttag ctactttcgt ccagttgact aaaagctccg
    28681 ttccaacatt gaatttgcgg atcccgttat tgatcaattc aggataatct tcttctttca
    28741 cacctgttcc tccatggata actaggggga catcaactac tgcatcgatt tctttcagca
    28801 agggaatgtt tacttctgtt tttgatttga attgtccatg attcgtaccg atagcaatag
    28861 ctaatgcatc cacgcctgta gcttctacaa atttcactgc gtcttccggt cttgtatata
    28921 ccttgtcatt ttcagcgaca tggattcctt cttctgtacc gccgattgtt ccaagttctc
    28981 cttctacgga aacaccgtgc gcatgggcat attctactac tgcttttgtc ttcaggatat
    29041 tttctttgaa aggcagatgg gacccatcat acatgacaga agtatatccg ctgtcgattg
    29101 cttctttgat atcatcaaag tctcgggcgt ggtctaagtg caagactgca tcaaccattt
    29161 cttcttctgc aatagctgtc attacattga ctaacacatg catgccgata tatttagcgg
    29221 tatcgacact tgtttgaata atgatcggcg aacccatttc ttttgctgcg cctagcatag
    29281 caggaagcat ttctaggtta tgcgtattaa aagctccaac tgtaaaattc aactcttctg
    29341 ctgtttttgt tacttctttc aatgttgtat acatctttta ttgccacctt atctttttat
    29401 ttgtttcctt tgatcgttac ttgttttgca atttgtcgca tctcatccat tttgcccatg
    29461 tagtaatcga tcccttgatc atcaccagca cgagcagcat ctgcacgctt ctggttatag
    29521 aggctcatca gttttttata cccatctccg atcgtctgtt tattttccaa actcttttct
    29581 aaatattgga tggcttgatc gttcacatac aattcagaaa atttaagtcc aacttgttca
    29641 taaagtttcg ctaatttatg cttatcttgg gtagtggaaa tctcttgttt caattgtgta
    29701 atctctgatt gaatcttttc ggtcgcttct ggcgataggg ctttttcttc ttttgccaga
    29761 acttttttcg gttctttttc ttttttctta aaaaatccaa acatcatttt ccctactttc
    29821 aattaaaatg ctttcaaaac tgggcttaag atccaggcat ataagagtgc tgcggctact
    29881 gtatctacat cggttgctgt agcattgatg aatcccatcg aattaaagat cgttactagc
    29941 aaagcgggca ataatgtaat aaagaaacca tgagcaatac cgccaatgat cgctcctcgg
    30001 cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc cggcaaagaa attggttagc
    30061 ataccaggta aaatcatcgc taagccgaac atgggcaaag taaacatggc aataatcgta
    30121 ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg ggctataagg aaagaaaact
    30181 ggacaatcta aggcagggat cgcatcagga actaatttca tagctatccc tcgaaatgcc
    30241 ggcacgattt cacctaacag tagacgtaca ccagctagca atacatacac acccactacg
    30301 aattggatgg cttgtaaaaa ggcaaacatg atatagtttt gatctcctga taattcagaa
    30361 gcgaatggtt cgcctgcaaa taaaacagtg atgatatata gcgggaccat cacgaccatg
    30421 acagacaaat aagtatcttg cagaaattcg aaatgtgtcg gcaacttgat atcttcgatc
    30481 gactttttat tttcgccttt ctcaccaaaa agcttagcaa caccggcttc aaataagtac
    30541 ccgatcgtac aaaaatgacc taaagcaata tcatttgatc cagtgatttt acgaatgatc
    30601 ggttgcgcta ctgccggcat agcaatggcg aaacaagctc cgataaaacc accgaccaag
    30661 atcaatacga tccctcgcag accggcaaaa tagccaaaga ctgtcgtcat cgttgccatc
    30721 cataaaattg cttgaccagt taaaaaaatg tacttccatt ttgtgaaacg agccaaaata
    30781 atattgaata caaaaatggc taagaacgtc agtgcaatat ctctacctaa acccaaatca
    30841 ttcattgctt gaccattgat agattcaata gaagggatga tcccttgcat atgaaatcct
    30901 tcagtaaaga ttttcccgaa ataagttaaa cttccaacga tgatgctcga accggcactc
    30961 aacacttgaa atcctaggag tgtctttaac gtgccagaga tgacttgtcc tgctgatttc
    31021 ttctgcagta gtagacccaa catagcaatc aatgcaatgg tgatcgatgc ctgagtcaaa
    31081 atattttcga tgataaaatt gataacgccc atgtttttac cctcttttct ttttgtattt
    31141 agataactcc cttatcttgc agaactgggg tgattttttc ttcaatttca ttttttgaaa
    31201 caatattttt caaatagatc atcgccgttt taggatcaac tgcgaacttt tcgaactgcg
    31261 aacgaaaatt ttctgctgtg atgatcacat ctggtttcat ggaaactgcg gaagaaatat
    31321 cacaatggtc cagtttggct tctactccta atttgttcaa aacatcctca gcagacatct
    31381 gtgctgcaaa actacttcct aaacctgcac cgcatacgaa taaaatgttc aatttgttca
    31441 tctttgttct cccccgtatc atttattatt tctcaactac tgaaaaaagc agttgtttga
    31501 aatcttcgat atttgtgcat tcaatcaatc gatccaactt ctcttcgttg ttgatcaatt
    31561 caattagttc tttcatgatg ttcagatgcg agtaggagtc tactgctgcc aaacaaaaaa
    31621 taatctttac tggatccatt tcaggattac cgaaatcgat tggttgctca atcgtagcta
    31681 cgctgactcc taatttattg accccatctt ctggtcgggc atgggctagt gccatgtgtt
    31741 tgccaatcac gatatacggt ccgtaatcat tcactgcacg aatcatcgct tcgacatagc
    31801 gctcttcgac aatgttttct ttaaccaatg gtctagcagc tagttcaatc gattcctccc
    31861 aagtagtggc tttttctttg atcatgatat tgctgtccgt caaaatatct tttaacatag
    31921 gctggatctc ccttttgttg atttctaaat tgtttgtttc gaataatttt tctaacttgc
    31981 catagatgat tcgatctact cggccaccac tttctttcat gacatctatt atttgagtga
    32041 ataactccgt cgattcatga ctgttaggct ctaaccgctg tagttcttca tttcttttca
    32101 aaaagtcttc tataaccaag atattcgtct cagttaaaat cggatcgaca actaaaagcg
    32161 gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag attgacatcc aatttatcta
    32221 ctaattcgat ttcttttgaa cttaaaactg cagtaatctc gatctgagga aacttttctt
    32281 tcagattctc tgctaacaga tttccagtag ccatcccatg gttacagaca acgactgatt
    32341 tataaacata attcaagtct cgcttgatta cactggcgat cgtcgaaaag tggatgacaa
    32401 gaaaagctat ctcgtcctcc agtatcttgc caccaattac ttcttcgatc gtaggaacaa
    32461 aatgagtgac tgcgcggtag attgggaaat aatttgttcg gatgttttct ttcaatggat
    32521 tcatgacttg gatatgattc ttgattctta caatcagtgc agccatgtgc ttatagagat
    32581 tttcgaataa agcttcttcc tttagatgga aaggtacgtg tgtctcgttc tctacaaatt
    32641 ggatcaattg gatcgttagt aattgagctt gtacccactc gattgaattg gagatatctc
    32701 tagcattgaa cgtctcaata gtgaaacgaa gataatttct ttctacttgc gggacattgt
    32761 ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga aaagctactg tcttttattt
    32821 ctgtattttt ccaattaatg gcagagataa attcatgttt cctgattcgc tggatccaga
    32881 tcaatgtaaa aagcaggatt tgttttttat aaatatcatc ttcttgtttc aacaacttat
    32941 tggaatagat ctcctgtatt tttttgattt ccaccaaagg aaaataatgg agaaagattt
    33001 tttgagccgg tgttaattgt gcatcttgat tcataaagaa gtcggtcatt cccagattct
    33061 gattgataaa atcatagatc atcgtacgga tagatcgttc tgaaccttta taaaccagtc
    33121 cttgctttcc ataactgacg atctctatcc cataactgac cagtgttgct cgtaaccgtc
    33181 tcatatcttc atctaatgta cttttagaga tttgaaattc ttcttctttt ttgtttagat
    33241 ggatcgggat tcggctgtaa gcaacggcta aaactagatc aaaaatacgt tcgcgccgat
    33301 tcaaaaagcc gtctttcaca tcttttattt cagtttctaa tcgagcttgc ttttcctttt
    33361 ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat ctccggaagc tttttactgc
    33421 tcaaccagat attcgcttca agaacttcct gacggatcgt ccgctgactg acattgaact
    33481 cacttgctag atgttttgct tttactggat attgggctgt catgattctg tgaagtattt
    33541 caattgttct tgttttcata gaaatgatct ccttctgatt tcaagtatat ggttccgctt
    33601 acataataaa aagtagatat ttctgccact tgaagtggaa agaaagtaag caaagcaaac
    33661 aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa agacatacgc ctattattac
    33721 aaaaattttg cccatctata caaaaaagag gttgtgaaaa agctgtcctg catcaagtaa
    33781 taagaacaac taaactaaaa tagtgcttcc tatttttcgt ttggttgttc ttattatcgc
    33841 agatgcaagc ttttaaacac catttagtgg agaggttgtg acagagatgt tcagccttga
    33901 gcgataagaa gcgattttga aaaatcgctt cccatatttt acaaaatcgt gccttattgc
    33961 cgataggcta tctctggcac actgtttatt cagaaaaagg agctgcgaca aggcttttgt
    34021 cacaactccg ttcataaata tttcctgtca atccagcata atcggctgaa taaaatcggc
    34081 ttgccatctt ttttctttct cagaaaaatc aaaataccat atctgagaaa aatactgtcg
    34141 ttgtgtctta cttcctgaga tcaatcgccc atcttttcga tcgactgaat aatcacagca
    34201 tctgaaatca attcggatgg caaaactatt ttcgtggatc tgtcgataat ttcctacttc
    34261 ctgaatagtt atttttttag tatgatttct tacaccgtct gctttgtttt tttgcagaac
    34321 tttctgatgt tcggcaaaca gcttgtctgt ataacagctt ctggcttttt caggagtttc
    34381 ttcttcccaa gcggattgga tcttcatgaa tatcttttgg atttccagaa actgtgcttt
    34441 cttttcttta gccgttcctg acatgcggga caatatctcc gctttcagcc ttttcttttg
    34501 tcttttattc ttgacgatag aaaaagcaga aacccctcct actgacaaaa cagctaatgc
    34561 aagaaatccc gtttgctcat aactagaagg acctgtacct ggaccataat acccataaga
    34621 atctctgccg taagaacgac tgccaaaata actgccccca tatgaacgac caccaaatga
    34681 acctctgccc gaacttcgac cgctagatgt acctccacca tgaccacccg cgacagcgaa
    34741 tccttgggtc ggcagagcaa acaaacatag gcccactaac aaaatgattg ctaaataatt
    34801 gatttttttc attgtcttcc tcgttatctt ttattacaaa aaactatttt tatgatagcc
    34861 ttgctaaaag gaagagtcaa taaccagatg cataaactgt ttttttagca attttgtctt
    34921 atttagtcat tgagtaacat cggacaaatg tatgtgtcgg cagtccataa ttcctctatc
    34981 ttcagcatga acaagcttgc agacaagaga tctgcgcttc ctccaggaga gatttggttt
    35041 tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg gtaaagcact ttgactagca
    35101 gcatgagcaa gtttttgcat ttccactaac gttttctgat cactgcgatg caatacgcat
    35161 gtgtcatcta gcgaactcat caaaactaac aacatatgca tttgtttctg tacatatgaa
    35221 tcattatcta gctccttagc tgcttggatt gcaagcctga tatgcggata ccctaatatc
    35281 gcttcttcat atgcaccatt tacgccatat ttttgcttca ccctatgacc atgagtggtt
    35341 tttttatgac gatattttgt atcaggaaaa gcagcaagct tgctgacatc atcaagtatt
    35401 ttcgataaag aaaaaggctg ttccatactg attttttgtg caatgacact gatcaatagc
    35461 cccattgccc aaatcgctcc tttatgggta ttgatgccat ttgtagcaat cagcattgcc
    35521 ttttccgctt ttcgcccaat ctcagcaatt ctttctctta actcctgatc aatagcatga
    35581 tgccatgcta tttgagccat ttcttcaaaa aaaggagtta gcgtcttagc agacttaaga
    35641 aataaagcat agtccatatc ggaatgtgca cccgcatcca gtgcatccac taatccaggt
    35701 tttggactta atgttacttc tgcgatcaat gcttcctcta ccaacgcagc gagttgctga
    35761 gtcactgctt tttttcgttc gtcatttatc atgacactca ctccttcttc ctactattac
    35821 ttgctgaaac cttttttgat agctacccaa atgatatgaa gtaaccagct taaagcaaat
    35881 gcacaacttg ccgtcattag ataagctaca agaatcatga ctcctgaagg taactgcagc
    35941 caactatctt taaacaataa taacagtact tggaaaacaa atacattggc taaatacgta
    36001 cgataagcat aaacagacaa ccatttgaac aatggcaatt ttggcgaatc gtttacaatc
    36061 attttggaag cgcctatgaa gacagcaaag atgatgacta aactgtataa actttggatc
    36121 gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg gatgtgcgag caatgaacgg
    36181 acagaaaaga acgcgataac taaggcaaca ggtaagaaaa gataacgtac tctgtccaaa
    36241 tattgataga ctttttcgtg ataaacaaat gcggctaccc cataaatcat gaaactagca
    36301 acaaaccgat ctaataagta ccagctgtca tgataaggcc cagtcaatac ttgtgtttga
    36361 tagaacagat accatgcgac atataaaatc gttgaaccaa tcaatactgg ccatacatat
    36421 tttttattag caaatacttt gtaaccgagc caaacaaaaa acggcatgaa caattggatc
    36481 tgcagcatca taacggtata ccataaatga ggcgctccat cacctgtgac atatttcagt
    36541 aaaaagcttg cagtatccgt atacggagtt tgttgttgga ctgctggaaa tagccaaaga
    36601 taagccgtcg tccatagaag atacggaacg actagttcaa aaaacttttc tttcaaaaat
    36661 tcccaataag acaaatgctg acttgttttt accatattat aaacaattgc aaaaataaac
    36721 attggtgctg tgtatttaac gatgacgtaa aaggtaccaa gaaaagctgt atttgttgcg
    36781 ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca tcataacagc cgttacagct
    36841 gatactttct ggaagtcact tacgttgatt ttgatttttt tgttttcaaa catctctttc
    36901 tctctctttt tctttattac caaagttctt tggttaattt aggtccttcg actgttttca
    36961 atagaatttt tttctctggc tctctcaaat actcttttag tgaaaaaccg ccttgccgag
    37021 tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg cgattgaatt acctgaattg
    37081 gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa atcactggtt tcttttactg
    37141 ccggaatccc agtcgataat tcaaatccta agctgccgcc gattccccat ttacatcctg
    37201 ataatatgtt tctagcagca tgaaattgct gataaacagg ataatccatg attttctctt
    37261 gtctatacga ttcttgttcg atgatgtccc acggatgaat gacttgtttg acggcatgtt
    37321 ttgagatttc tgttgaccat ctctgattgc gggcaggtcc acgtaaacca atcggtagta
    37381 tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa catcttttca gttagaacga
    37441 cccattcagg caaggtgaac ccttctattt catgtttatc aaaaaggaca atatcatgtg
    37501 cttgaatcat tttttatgca tcccattctt cgtccatttt ggcacggact ttatttgttt
    37561 cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg atttgtcagc atcgtgtcat
    37621 ttgtattgcg gacatcttga atggcttcat tgattgcttc gatgactttg ctgacatttt
    37681 gttctgaagc agcttggtcg gtgatatctt ctaggaaatg atagatcgct cctagttttg
    37741 tataattatt gatatcgtaa gcgatagctg gaactttttc agcagctgct tcgatttcag
    37801 caaccgaacg ttttgtcaca cgtgcagcac tttctttaga catcgcttgg acggtaattt
    37861 ctttattgtt taaagcaatc aagcggcttg attgtaatcc atgtgctaaa aatgcacctg
    37921 aaatagcatt tcctgcaatc aatccaataa ctggatgtcc agcttgacgg agttttgcat
    37981 aagcagcagc actattagcc aaagccacat ggataccgat caattcttcg ttatagccgt
    38041 atgcttggct tggtacatcg atcacaacga tgattggccg tttcactttt ttgtctttat
    38101 cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa accttcaact aaaccaactt
    38161 caccgtttcg tacacggtac atcgggtttt taggatcagg tacgattgat gtcacgatgg
    38221 cttcttctat gaatttactt gatttaccat aatacacagt actaatagaa gaagttggat
    38281 ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac acttgctgca gctggttcat
    38341 ttccttctgc tacttctggt aagtcaattg attttgcttg atggttagca attgcttcat
    38401 tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga atcaagtaat gataaataaa
    38461 atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc cattgctgcg atgacagcag
    38521 ctttgattgc ttctacacta tcagtcacta attcatcaat gatttttgat tctacccgtt
    38581 gacgagatcc taatgtattc caaatcaagt ctttgtcact agaatcaaat tctcgaacgc
    38641 ctgcttcttg ttcaataact tctggtccat tcaaaccgac tctcgctttt tttgttgcga
    38701 ttaaataact cattaatgct gaagtaatcg acataccgcc aaatgatcct acacgtcctg
    38761 gaatcaaacc gatgactggt acatattgtt ttagagcaat gattacattt ccgatttctg
    38821 aaatagataa cagtccgtag tttgcttctt gcaaacgaac accacctgta tctagtacaa
    38881 tgactggaaa gatctcattt ccttttttat tttcttctaa cacatgttca agtgcagcaa
    38941 cgatttttgc accggaaact tcaccgatac cgccaccttg gaattttcct tccatagcga
    39001 ttactactgc ttgtttgcca ttgatcgtgc cacggctgac aacgatcccg tcatcacttt
    39061 cagggacgat tccttgtgct ggtaaacttg gcgcgatgat ttgatcgaat ggatcaatca
    39121 attcacgtcc attgtcaagt agagcgttca ctcgttctct ggcgtttaat tctacaaaac
    39181 tatttttcat ctgaaagttc ctccattgct tgcgtcaatc gtaggtacac aactcctggt
    39241 gtagcaccga agtcattgat cacgatatct gctgaaattg gataacgatc aaaaaatctc
    39301 gttaatacat tgttccatac ttcgccgaag ccatcgcttc ctgtacaaat ttttacagta
    39361 gattctgttt tttctgttgg aaacatcaag atttccaaat ctcctgaacc gaccactcct
    39421 acatggattt tcttattgat cggctgtttc gttggatatt tataagctaa ttgttccatg
    39481 gatctctccc ctttttgtaa aacagagcat acacgttctg cttattcgtc tttcttcttc
    39541 catctgggta aatggcgaca aaaggttttt ctcgccaatt ttatgaccaa gttctgaatt
    39601 tcattggtgg ttcatacagg ccatctgacc attcgatcaa atcatcgatg gtttgtgcag
    39661 ctaataatga acgtttcgct tcactacgtt tgatgcctag atcttctggt aatttcacga
    39721 tgccggctgc acgcaagcta tctagtgatt ctttcgtgct agtcattcca agaggtgtaa
    39781 caccgccaat tgctgcaatt gctgcttgtc gttcttcttt tgtttttgct ttgtacaaat
    39841 aagcaattcc ttcttctgtc acgatatgtg tagtatcttc gctataaatc atcactggtg
    39901 cattggctaa gcctgcttgt ttttgaacag ctagtgcatc taattcttcg acaaagactg
    39961 gttttttatt tgaaccatat gtttcaacca tttgaacaac taatttttgg cctttcccta
    40021 atggatcatc attgtcgcga agtgattgcc atgctggtgt agaatgacga cgaccgccag
    40081 gattatgtcc catgttaggc gcaccgccaa aaccagataa acgtcctttt gttacggtag
    40141 aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat aaacatatcg atggcatatt
    40201 gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt gccgtcttta ccgacaaaaa
    40261 agacatctgg gcgagcagca atatattttt ccataccaac ttcgccacca aaactatgga
    40321 tgctttcaac ccaacctgat tcgatggctg gaatcatcgt tggatgtggg ttcaataccc
    40381 agttcttcgc gattttccct tttaacccta gtgattcacc ataagttggc agtaacaatt
    40441 cgatagctgc tgtattgaat ccaataccgt gattcaaaga ttgtacgccg tgttctgcgt
    40501 agattccttt gattgccatc attgccatta gaatttgcaa ttctgtgatg ttttgaggat
    40561 ctcgtgtgaa taaagcttct aattgataag gtttatctgc tttcacgaca acgtctaccc
    40621 agtcaccagg gatatctaca cgtggtaaag aatcgacaac ttcgttgact tgaacgatca
    40681 cgataccatc tttgaaagct gcagcttcga tgattgttgg tgtttcttcc gtattgaaac
    40741 ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc tactaaaaca acatttggaa
    40801 tcaaatcgat gaataagcgt ccataaagtt ccaaataagt atggatatca cctaatttca
    40861 ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg tgcaccagca taagagaaat
    40921 cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg ttctggtcgt gagatactag
    40981 acatgatcaa atgcagatca ttcacttttt ctgcatctac tttttcaaga ctttctgaaa
    41041 gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac tttgtcgccg ggagcaatca
    41101 gtttttccat tacttctacg attttttcag tttctacata cttgccattg agcaagttgc
    41161 tgattttttc caatcgctgc gctttttttt cgcgtttaga agcccagttg cggtttggtt
    41221 tttccataat tagtcatctc catttttgat atagaaacgt ggcgtcatca ataccatgtt
    41281 ggtctagatt gatcgtacgg atccctttat ctacaccaaa ttttgtatga atcagctttg
    41341 tcaaagtatt gccgggggga aattcaatcg tcagatccat tcctaattct tttgccaagt
    41401 ccatcatttg gttccactgc acagggaaag ctaggttttc caacaaatct ttacgaatct
    41461 cttctgcgtc ctgagtggct cttccatccg tattttttat atacaaacat ctagcatcag
    41521 atagatggac agactgcata tacggcttga atttcttgac cgtatcattc atcaagagac
    41581 agtgagatgg cacagggact ttcagcagct tcgctgtttg cgcttggtgc cgtttggctt
    41641 ctgccaacgt aacggataaa ccttcgatac tcccagataa cgtatgttgc atcggacaat
    41701 tttcatttga cagataaact ggatggtcag gatcaaatgt ttttgctaca atttcctcag
    41761 cttcgcttct cgtcacacct actactacgc ccattgcata accgcttgga taagcttctt
    41821 tcattaagac tgcgcggtga tagactagtt ccgctgcatt ctcaaatctc aaagatcgac
    41881 aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc tgcaatatca ggctgaatac
    41941 cgtttttttc catttcatct aaataaaata ctgcttttgt cagtaatgct agctggataa
    42001 aaaccgtatc ttgatagttt tcttctttat caactaaccg ataccccgtc acattttcta
    42061 aacgttccat gtacatttca gggatatccg ctaacatccc taacttctga ctgccttgtc
    42121 caggaaataa gaaacctgta ctcatgagcc tcgcctcctc agcacttact ttttccactg
    42181 ctatcttagt caatttctgc tataataaaa aatgccaaaa aaagaaaaaa ccataacaaa
    42241 aaggtatgga tgggatggat aattatgaaa ttgcttcatc tgcagtattt taaaaaagta
    42301 ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat ttatttctca acctgccttg
    42361 agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc ctttgtttta ccatagcggc
    42421 agaaacattg agacgaccgt ttatgcagaa gaattttatc catatgcagt caagacatta
    42481 gacacattag aagaaggaat caaattattg tcttcggtca atgagaagat cgttacttcc
    42541 gtcatattgt atttagaggt agcctctgtt tctattccga acttggtccg aatttttatg
    42601 gaaaagcatc ctgatatcca attaacgatc atgcaacatc atttaccaga tgatattacc
    42661 gatcctgtac tgtacatcac ttcggagatg aagcctggca tgaccaatat tcctatcatg
    42721 aaagaacctg tttatgtcgc cattcctaag aaacatcctc ttgctaaaaa agaacatctg
    42781 caattagaag atatccaacg cacccccatc ttgatgctgt caaagagcaa cgccttccgt
    42841 catacactgg atgcagcact tgaagcgaaa agtatcgaat tgacgatcag ttcgacgaca
    42901 gatgatcctg cgacattacg ttctattgtc aagcaaggat tagctgttag ctttttccca
    42961 aaagtttctt ggtcttacga taaaaatgat cctttcgtct tgcgggaaat caaagatttg
    43021 cccttgacac gaacaattta tttatcttct agcttcacag aagatcatcc tttgatcaaa
    43081 atgattgcaa aaacgatgcg ggaattttat ttaggaaaat aaaaaaaaaa gagctgggac
    43141 aaaattcttg tcgcagctct tttttcgaac aaacggtgtt ccagaagtaa tttcttagaa
    43201 aataagccga aatttcccaa aaatttgaga agcaattttc gtaaattcct tcttatttct
    43261 tgaagctaaa catttctgtc acaacctctt ctctattgtc ctagacattc attgcgttga
    43321 tttaaaactt ccatcaatca aatctgctag ttcatctagc ttttctattt tacgtagatc
    43381 aatagagatc caatgttctt tattcatgtg ataggctgga aaatacccct cttttcctcg
    43441 caaaattccg ccaagttcag gatcgacctt caaatcaaga atattgatga tcctatggtc
    43501 acttgcatca tcatagattt tttcttctgg aacagacatc aggaccccaa accatttttt
    43561 gcttttttga tgtctgaata cgcaataatc aggaaatttt ttaaaaatat attctggaga
    43621 caaattgtat gtttcattga tgtgatcgat tagttctttt ttcgttagca tctgcttcca
    43681 tctctttctt attgattcgc tttattcaaa gataccacgt aagaatagaa aaatgaatac
    43741 aagtagttgc taatcaaata cttcgatatt tttctttttt atcaactctt aaatgttcaa
    43801 tccgcctgtg ttggtcttgc taccatctac tattaaaatg tttattattg tttgcccacc
    43861 tattttttga ttttagtggt atcatttttc aagttcatga tgaaattctt atcatcagta
    43921 aatgctccag aaaaataaac aatgattgaa aaatcagttg aaacttgacc accgtttatt
    43981 ttctccgtat tcttagaata gaagggagtc tgattgatgt attcactaat gaaaaaaatc
    44041 attaccgaaa aagatattcg acgtcatgtt tctttagtag aacaattgct gaatcataca
    44101 aagataaccg taaatgaatt agctgaaatc atagggacaa ccgaacgaac gattttttct
    44161 gaccttcaat ctattcgctc tcagctgccc gaaggctggg acatcatcag cgatcaagca
    44221 ggaatcagtt tgcaaaacca gcaaaattta ttaacaaatg acctttggga gatttttttc
    44281 aaacaatcag tcagtgtcga actgctgaaa aatctgcttt ttacaaaaaa agttgctgtc
    44341 cctgattttc tagccgacca tgggctttct tatgggacat tgaaaagaca tgtgactaaa
    44401 atcaatcagc gtttagcaag ttacgacttg cagatcgatt tgacgaaata tacagcttgt
    44461 attcttggaa aagagcgtgc gattcgtact ttttatcatc gattgctgat tcctttcact
    44521 cataacaact attttttcga ggattattcg atccatgaat cccattattt tcaattttta
    44581 agaaacctca gtcaaactga actagcggtt gaaacagaag agattttcgg aacatgttgg
    44641 ttctttatca atacgatccg tataaaagcg aattgccgat tagattcttc tatccatacc
    44701 aacagcacac tctcctccct gtatgactct gcattgaaaa aattgtattt aacggaaggt
    44761 atttatctta aagacactga gctttctttt gcttctttct gctttctgga aagttggaat
    44821 tacaacaata actatggaca agaaaccgct cgctgcctgc atcattctcc atttttagag
    44881 gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt ctcttgatca actaaaaaaa
    44941 acatctctga ctgataactt ggctctcctc atattaaaat atcatgaatc gccgatattg
    45001 attgaagcat tggaccgtca ataccatcgt tttttagaaa catatgatca gcaatatgca
    45061 cacttgtatc aacagaaaga gaatttactg aacgatctaa aaaaagaaat agaaatcaat
    45121 gaaccaaatt actttctcca actcttgtct ctactgatcc aacaaacgat tttttctatc
    45181 aaaccgaatt tgttcaatgt ctattttatt ttccaaggag aaccttcatg gaaggcgttc
    45241 ttgcagcagg aattaaaaga ttacttagga aaacgtgtct gcttcttacc tatcgaacta
    45301 accgatcttt ccactatttc ttttgaaaaa acagatatct tgatttccaa tttccctcta
    45361 gattctctag atatccctat tgtatacatt tcttctattc caacgaaaaa tgagctcaat
    45421 cgtttgactg aactcacttt caaaactttt ttatagtaac ggctagcgac tgtattctct
    45481 agctgttttt tcttttttgc gaaacagttt gttcatgatc cctttttcta tttctacaac
    45541 taagaataga agaatcacat ttgctccaat caataactgt tgtgtcatcg ttaaacttac
    45601 tgtaccgatc agctgctggc cgactggtag aaaaataacc atcccttgca ataaagcaag
    45661 gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga ccttggttca atgacggctt
    45721 ggacaactct ctgcaattga tcatgtaaac agcttgagct aagacgatat tttgcagaag
    45781 aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc aataaatagg caggaaccat
    45841 gatcagtagg gagacataga tgatccgaaa caaatgataa ccagatagga ttccttgctt
    45901 tggatctctt ggtgggcgct tcatgatttc tggatctggc gactcgaacc ctagtgcata
    45961 agataaagtg attgtcgtta ccatattgac ccataaaatc tgtactgggg acaaaggtag
    46021 cgggcgattc agcataagtg cccaaatcaa gatcaatcct tgagctaatg cagttggcaa
    46081 gaagaaattg atggtttttt gcaaattatc caaaatccgc cggccctctt ttacggcttt
    46141 tgcaatcgta tggaaattat catctcctaa aaccatatca gctgcctgtt tgcttacttc
    46201 actacctttg attcccatag caattcctac atcggatctt ttcaaagcag gtgcatcatt
    46261 tacgccgtct ccggtcattc cgacgatttc tccatttttc tgcaaagctt taacaattcg
    46321 cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact attggcactt gctgaatcaa
    46381 ctcttcatca gacataagat ccaaatcgat cccttctaaa actttggcag tatgttttaa
    46441 gccaatttgt tccgcaatcg cttttgctgt gtctttgtga tcccctgtaa tcattttcac
    46501 tgagatccct gcttgttggc attcctttac agctttgact gcactttctt ttggcggatc
    46561 gatcaatccg gcgatccctg caaaagttaa ttgggttaaa gtctcatgcg tgagttcctg
    46621 tttcgaatct acttttttat aagcaaatcc taaaacacgc tgaccttttc tcgcaaattc
    46681 tgcagctgtc tgactccatg cttcttgttc tgcagaagaa agagttgatt tagcaaataa
    46741 tacttctggt gcacctttga cgtaaatcac cgctcctttt tcctccgcat gcatcgttgc
    46801 catatatttg tattcagaac tgaacggaat ctttgcttct atttttttca aaggtagatt
    46861 ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga ttgccactta aattttctgt
    46921 tttctgttct tcttttgttt ttaattcctg acagtttttc ataatggaca acacgcaggc
    46981 ttcttctttt gcagctacat ccacaactgt catttcattt tttgtcaatg ttcctgtctt
    47041 atctgaacag atcactgtca tagaacctaa tgtttctacc gaaggaagtc ctttgatgat
    47101 cgccttttct tttgccattt catgtactcc aacggataga atcatggtca aaacagctgg
    47161 caatccttcg ggtaccattg acaccaccaa agcaatcgta gccgaaagaa ggatatgcca
    47221 atccatacca tatcgaaatg tcgtaaaaat aactaagaat aggctcaaac aaagaatgcc
    47281 ttgaaaaatt tgtttgttta atcggttgat ttttttaatc agaggcgtcg tttgttgttc
    47341 gaccgattgc aatgcttgat tgattttacc gatttctgtc gtgtttcctg tttctacaac
    47401 gacaccgact gcagaaccag attgtacaag cgttcctgaa aaagccatat ttttctggtc
    47461 gccgatcgat tcttcttctg tcaaaacggc atgacttttt tcagatgctt ctgattctcc
    47521 cgtcaaaatc gattcttcca ctaataaatc atgtgcttcg attaatcgga tatctgctgg
    47581 aacgacatct ccggcacgta agacaatgac atctcctttt accagttctt tcgcagggat
    47641 tttttcttgc atcccgtttg atagtactat cgcttcttgt cccatcagct gcttcaaacc
    47701 attcaatgat tcttccgctt ttctttcttg ccagtaacta acgagtccat tgacgatcac
    47761 tacgaataaa ataatacttc cttcaatata ttcactagaa gcaaatttca aaaaagaggc
    47821 aatgatcaac acgatcatca gcagatcagt aaaatgcttt gctatttttt tccatcgttt
    47881 tgtctgctgc cttgctttta tttcattata tccttgatca gtcaggcgtt tctgtcgttc
    47941 ttcttgcgac aagccttctg ttgatgtttg atagtttttc aatacatctt ctaccgtcaa
    48001 tctgtaccag ttcatcctat cccctcctgt ttaattgata ctgttattat acgcagggaa
    48061 gttttttcag gcgtatcgat tatttcaggg gggtctgaaa taattcgttt tgcaaaatgt
    48121 atctgggcct aactgatatt tctattgctt attcttttat atacaaaatt taagaataca
    48181 aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc tttcgaataa caagctgaaa
    48241 gcaattgaag taaaaaacac caacaaagca agatctttat atctttgtgt agtgttccgt
    48301 ttcttttctt taataaattg aacatgtgca cacaagatcg aagaccacac aaatagaaaa
    48361 caactcgttg tcatgcctgc caaaagctga aaaaacaatc gactatcagc tacaaaaaaa
    48421 tgtagacaaa gaccggcaaa aaataacatt cctgtaatta aaataccgtg taaaggtacg
    48481 cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt tttcttttgc tgtttgatat
    48541 aaaatcctgc tagtactgta gactgcacta tttgctgagg acaaagaagc aaccaaaatc
    48601 attagattga caattgctgc tccgccttgg caatggatca cttgcagcat agtcactaaa
    48661 ggactttctt ttaaatctaa acaatgccat ggaacaacaa ataaaatcga caaaatcgtt
    48721 cctatataga aaaagagaat acgccaaggc agacagttga ttgctttttt caaattcttt
    48781 tgcggatcac ttgtctcgcc aacggttaag ccgatcattt caattccagc aaaactgaat
    48841 aaaaccatag gaaatgagac taccattcca gcaaagccaa acggtagtaa ttcatcgatc
    48901 tgtccaaaat tttctttgat tccgatagac tgggtatgaa aatctgagac actcaatcct
    48961 atacccaata agataaaaag gaaaatcccc aagatcttga tgattgagaa aaaagattcc
    49021 aattcaccaa accacttgac tgcaacaagg ttgactgcca ttaagatcat tagtgcgcta
    49081 cctccgctta tcaaagctgg tacatgtaca aaccagaaat ggatatacaa tcctattgct
    49141 gttaattccg caagtccagc tccaagccag caaagccaat aagtccaatt gacaaaaaat
    49201 gcccactttt tgcctaaata atagtcaacg aaatcactga tagagttaaa tgtactattt
    49261 gctaaaaata aatcacccat ggcccgcatc aaaccataaa tagccaaacc gcatatcaaa
    49321 taaacgagaa taacagctgg ccctgctgat gcaattgcct ttgctgaacc taaaaataat
    49381 cctgttccaa ttgcgccacc taatgcgatt aactggatat ttcgattagt tagagtccgt
    49441 ttcattgccg gtttttccat caagttcacc tttttctttt tcaagcagca tggcgacttc
    49501 ctgttcccaa gaaggtttct ttattttttc aatacagagt ggtactacaa acaaaatgac
    49561 actgatggct gtgactgcaa ttagattgat ccatgttcca gctttcatcg tcagtccgac
    49621 agaaaatccg attgccgcaa ataaaacaat aacgacagtc caaactccaa gattgctctt
    49681 tttctctttt tttccaattc taaatggtcg attcaacttt gcatgtgtat aacgcaatct
    49741 caaaactgcg atccccatga tgacatagac gatacagtag ataacagtag ttgaatttac
    49801 gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa taaaaaagtg caaagattga
    49861 gataatgaca gactgagtca aaataacgga tttggatacg tcaaattgat tcgtcttcca
    49921 aaaattgaat tttggtggat acgcaccttt tctagcactt tctgtcactg tctttgctgg
    49981 tcctgaggcc caagcagata attggactgc tacaccaatg aagacaagta aactaaacag
    50041 attcacaata agatgtggca aaccaagaat ttggcaataa atcgaaatag attgcgcgat
    50101 attattcaat tccatttgtc cttttggtac gacatttgct acaactaaag cattcaaaat
    50161 attgacgaaa aatataaaaa ttaacgcggc aaatactccg cgaatatacg ttttaactgg
    50221 attttccaat cgagtgatat acacagagga catttcgata ccagtaaaaa taaacatgat
    50281 cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct ggtattaatt tgtcccatga
    50341 aaaactgcct aatgttccgg ttggtacgat cccaactttg atccacgcag caaaccctaa
    50401 taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa acaccgactt ttccgccgat
    50461 ttttgccata tcaaatttca aattcaaaag agtgatgatc caataaacga ctaaaatgac
    50521 aactaatgta aatttgctgt tcaaaccaag cggcacatta tcgataatat taccaaacaa
    50581 cggagcgagt gctgaagcga ccatcaccat cccaggaaac atttggaccc ataacagcca
    50641 tgagacaacg aacccccatt tttttcctag agcattcgat acccaaagtt ctggaccacc
    50701 tttttgggga aacattccag caaattcacc agagatcaat gagattggaa aagcgtacaa
    50761 taatactgca accagcatgt aaaaaatcat cgtccagctc gttgctgcta catctggaat
    50821 atttcgtaca cttgctacaa gggcgcaggt cattccgata aaggtcatgg tcgtgatttg
    50881 ctttgatgaa ccagtatctt tcatctttat tccttctttc aatagcgaca tatgtttttg
    50941 ttttctttta tttttgttca agaacagaaa tgacaaggtt tcatcgattc agtacaaaac
    51001 ccagtatcac cgaaacatcc attgaacgat cttagtgaac tgggtttgat acatgaaacg
    51061 aacgatccaa cttatagaac gttttataaa aaagaatatc ttggcgagga atatatgaaa
    51121 aacaggggga aatacaattg ttttgatgaa gcaaataatt gtctgttctc catatgtctc
    51181 ttgttattct ttcacacaaa aatcagttct attggatgat ttcgtttagt ttcttttcca
    51241 ttgctttggc gatttttttt gtataaaaat tgaaaattcg atcatcattc aaataaggag
    51301 tcatgatagc cgcacgtagc agagtgattt tctgttcttt tttccattct tctttcgtta
    51361 gacccattct ttcaacaaaa cgaatcggag aatcaccgta atcttcttgt gtaaatgtag
    51421 tatgagaagt aataaaccgt tcaccataaa catcgccatc catataagaa gaccgatcga
    51481 acattttttc atttaattca ttgatagcgt tcaaatctgt acagccttgt tctttgaata
    51541 cccaattgac catattaaaa tcaggatgat tcaatggata gacttcgatt gtttttcctt
    51601 taacagtaaa agtcagatga tcgagaaatt ctctgaatct ttgagctgcc tcgattgaag
    51661 ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa tacacgatgt gctgtccaga
    51721 ctgcggcggc ggttgctcca gcttttgatc cttccaaaat ataagctcct aacatatctg
    51781 gagcctttac tgatttttca aacacatatg gcgcgaaata agaaatgata tttctcatat
    51841 ttttatgttt gattacgatt cctccagcag cataagggac atagcccatc ttatgaggat
    51901 ctatcgtgac agaatccgct tctgaaatag ctctgaaccc ttcatatacc tctttgtcaa
    51961 tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa ttcagctaag gaagcatacg
    52021 gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc atagccgcca tatgcggcat
    52081 caacatgtaa atagaaatag atcccttcat cttttaacct ttctcttaat tggacgattt
    52141 tatctacact gtctacttga ccttcttctg tcgttcctac gacagcaacc acacctagaa
    52201 ttgggatttt ttgtccagct aattcacgaa ttgttttttc caaagcatta atatccattc
    52261 gataatcctc ttgaactggg atagcaacca tctgatctaa gccgactccg caaatatcta
    52321 acgccttcat ccaagaatag tgttttgttt gagggaccag ccatttccct aatctttgga
    52381 tattttttcc gctacgggaa gaagctgctt tgacctcatc catttcttcg tccgtaaagc
    52441 tttcggtcat ttccaaaatt tcttcaacag ataaattcaa aagttcccac tcagacattt
    52501 ttttcacttt ttcaggcagc acttccttga cagctaaagg aatcgatttg atgcaacggg
    52561 cgtaccataa tccttcaaga ttagcaatag aaccatctgc tgcaatgtgt ccccatccgt
    52621 ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc tgcttccatt tgcgaagttg
    52681 ccatagaaga ttccaatgca acattatttg ggttccacag catcgcatag ttgtaagcta
    52741 ataaagcagg catcaatgtt tcagcattca tttgtcccca gtaacggcct gctgaatgcc
    52801 atgggatcga tcctgcgcgt aatcgctgac tgacttcatc caagacctct atcatatggt
    52861 cgcgggtggc tagataatct ggagtgagct tatctccttc gctaattgca ggcatatctg
    52921 aaggaatata attttctctc catccaagat gttcatctac catcttgttc aacaacattt
    52981 tataaactgg tccattttca gctttgtcgc ctataaagac ggccttgata tccatatctt
    53041 tcatttttct tccctccata ttttgttggt caatgtcttg cagatacatg tgtttatctg
    53101 taacacctct tcactatagc gccaaaaatg agaaattttt ttttataaca tcaaatccac
    53161 gtagattttt acaaacaaaa ataaataaag tcggattttt ctgataattc aacgcaaaaa
    53221 agcgaacaat ttttcaataa atcagtcttc taaaatagat aacctaaaaa aag
//
LOCUS       DADOOS010000005        50233 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.005, whole genome shotgun sequence.
ACCESSION   DADOOS010000005 DADOOS010000000
VERSION     DADOOS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 50233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.005"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            277..1602
                     /locus_tag="J8C37_000241"
     CDS             277..1602
                     /locus_tag="J8C37_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAZ5303709.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1595..1945
                     /locus_tag="J8C37_000242"
     CDS             1595..1945
                     /locus_tag="J8C37_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303710.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1942..2121
                     /locus_tag="J8C37_000243"
     CDS             1942..2121
                     /locus_tag="J8C37_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303711.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2257..2547
                     /locus_tag="J8C37_000244"
     CDS             2257..2547
                     /locus_tag="J8C37_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAZ5303712.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2915..3703
                     /locus_tag="J8C37_000245"
     CDS             2915..3703
                     /locus_tag="J8C37_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ5303713.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3690..4016
                     /locus_tag="J8C37_000246"
     CDS             3690..4016
                     /locus_tag="J8C37_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303714.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4365..5405
                     /locus_tag="J8C37_000247"
     CDS             4365..5405
                     /locus_tag="J8C37_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAZ5303715.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6241..6573
                     /locus_tag="J8C37_000248"
     CDS             6241..6573
                     /locus_tag="J8C37_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAZ5303716.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7164..7493
                     /locus_tag="J8C37_000249"
     CDS             7164..7493
                     /locus_tag="J8C37_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303717.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7480..7821
                     /locus_tag="J8C37_000250"
     CDS             7480..7821
                     /locus_tag="J8C37_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAZ5303718.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7935..8174
                     /locus_tag="J8C37_000251"
     CDS             7935..8174
                     /locus_tag="J8C37_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303719.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8241..8480
                     /locus_tag="J8C37_000252"
     CDS             8241..8480
                     /locus_tag="J8C37_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303720.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8566..9237
                     /locus_tag="J8C37_000253"
     CDS             8566..9237
                     /locus_tag="J8C37_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAZ5303721.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9290..11266
                     /locus_tag="J8C37_000254"
     CDS             9290..11266
                     /locus_tag="J8C37_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAZ5303722.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11279..11935
                     /locus_tag="J8C37_000255"
     CDS             11279..11935
                     /locus_tag="J8C37_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ5303723.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            12031..12858
                     /locus_tag="J8C37_000256"
     CDS             12031..12858
                     /locus_tag="J8C37_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303724.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12868..13116
                     /locus_tag="J8C37_000257"
     CDS             12868..13116
                     /locus_tag="J8C37_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303725.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13147..15258
                     /locus_tag="J8C37_000258"
     CDS             13147..15258
                     /locus_tag="J8C37_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAZ5303726.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(15296..17353)
                     /locus_tag="J8C37_000259"
     CDS             complement(15296..17353)
                     /locus_tag="J8C37_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAZ5303727.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17467..17679)
                     /locus_tag="J8C37_000260"
     CDS             complement(17467..17679)
                     /locus_tag="J8C37_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303728.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17866..18333
                     /locus_tag="J8C37_000261"
     CDS             17866..18333
                     /locus_tag="J8C37_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303729.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18330..18572
                     /locus_tag="J8C37_000262"
     CDS             18330..18572
                     /locus_tag="J8C37_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303730.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18590..18892
                     /locus_tag="J8C37_000263"
     CDS             18590..18892
                     /locus_tag="J8C37_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303731.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18963..21017
                     /locus_tag="J8C37_000264"
     CDS             18963..21017
                     /locus_tag="J8C37_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303732.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            21017..22849
                     /locus_tag="J8C37_000265"
     CDS             21017..22849
                     /locus_tag="J8C37_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAZ5303733.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23544..25460
                     /gene="ltrA"
                     /locus_tag="J8C37_000266"
     CDS             23544..25460
                     /gene="ltrA"
                     /locus_tag="J8C37_000266"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAZ5303734.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            25577..26377
                     /locus_tag="J8C37_000267"
                     /pseudo
     CDS             25577..26377
                     /locus_tag="J8C37_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26374..28905
                     /locus_tag="J8C37_000268"
     CDS             26374..28905
                     /locus_tag="J8C37_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303735.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28955..29287
                     /locus_tag="J8C37_000269"
     CDS             28955..29287
                     /locus_tag="J8C37_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303736.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29288..29881
                     /locus_tag="J8C37_000270"
     CDS             29288..29881
                     /locus_tag="J8C37_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303737.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            30044..31738
                     /locus_tag="J8C37_000271"
     CDS             30044..31738
                     /locus_tag="J8C37_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAZ5303738.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32426..34303
                     /gene="ltrA"
                     /locus_tag="J8C37_000272"
     CDS             32426..34303
                     /gene="ltrA"
                     /locus_tag="J8C37_000272"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAZ5303739.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34434..34811
                     /locus_tag="J8C37_000273"
                     /pseudo
     CDS             34434..34811
                     /locus_tag="J8C37_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34811..36016
                     /locus_tag="J8C37_000274"
     CDS             34811..36016
                     /locus_tag="J8C37_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAZ5303740.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            36032..36676
                     /locus_tag="J8C37_000275"
     CDS             36032..36676
                     /locus_tag="J8C37_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303741.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36687..37493
                     /locus_tag="J8C37_000276"
     CDS             36687..37493
                     /locus_tag="J8C37_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303742.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37515..37745
                     /locus_tag="J8C37_000277"
     CDS             37515..37745
                     /locus_tag="J8C37_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303743.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38291..38566
                     /locus_tag="J8C37_000278"
     CDS             38291..38566
                     /locus_tag="J8C37_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303744.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38608..39030
                     /locus_tag="J8C37_000279"
     CDS             38608..39030
                     /locus_tag="J8C37_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303745.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            39049..40641
                     /locus_tag="J8C37_000280"
     CDS             39049..40641
                     /locus_tag="J8C37_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303746.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40653..40988
                     /locus_tag="J8C37_000281"
     CDS             40653..40988
                     /locus_tag="J8C37_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303747.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41135..41329
                     /locus_tag="J8C37_000282"
     CDS             41135..41329
                     /locus_tag="J8C37_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303748.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41361..43733
                     /locus_tag="J8C37_000283"
     CDS             41361..43733
                     /locus_tag="J8C37_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303749.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            43794..45254
                     /locus_tag="J8C37_000284"
     CDS             43794..45254
                     /locus_tag="J8C37_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAZ5303750.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            45265..47469
                     /gene="topB"
                     /locus_tag="J8C37_000285"
     CDS             45265..47469
                     /gene="topB"
                     /locus_tag="J8C37_000285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ5303751.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            47715..48308
                     /locus_tag="J8C37_000286"
     CDS             47715..48308
                     /locus_tag="J8C37_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5303752.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            48324..49223
                     /locus_tag="J8C37_000287"
     CDS             48324..49223
                     /locus_tag="J8C37_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAZ5303753.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            49226..49711
                     /gene="ssb"
                     /locus_tag="J8C37_000288"
     CDS             49226..49711
                     /gene="ssb"
                     /locus_tag="J8C37_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ5303754.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            50074..>50233
                     /locus_tag="J8C37_000289"
     CDS             50074..>50233
                     /locus_tag="J8C37_000289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303755.1"
                     /translation="MMNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIID
                     KLLIMDDGK"
BASE COUNT        18562 a         7850 c        10144 g        13677 t
ORIGIN      
        1 tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact ccaaaaaagt
       61 ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt agattttcaa
      121 attaattagt aaagtgatag aataaattga tcatacaaat cataagaaga agcagggtga
      181 tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca acatcgaaca
      241 tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc aaaagagcca
      301 gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga atgtatagaa
      361 agaaatttag atccattaac aacagaactt gttgttatga gtcgatctga taatactggt
      421 tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat tacaaatgtg
      481 agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt tccaccacgt
      541 atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag atatgtggct
      601 gatgaagatc tactgattta ctcgatcgat gaatcgattt taaaagtgac aaaatcactg
      661 aatcttttta cgactgaaga aacacgaagt cagcgaagga agaagctcgc tcaaatgatc
      721 caagaacgta ttaaagaaga gttaggtttg attgctacgg taggcgtcgg cgataatccc
      781 cttttggcta aattagcctt agacaatgaa gcaaagcata atgaaggatt tattgcggaa
      841 tggacgtacg aaaacgttcc tgagaaagta tggaatattc ccgaaatgac ggacttttgg
      901 ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg gaattttgag cattagagac
      961 cttgctaact ggaatcctta tacgattaag aatagattag gtgtgattgg attacagctg
     1021 tacttccatg ctaacggaat cgatcgaaca gatatagcaa tcccgccaga accgacaaag
     1081 gaaaaatctt atggtaattc acaagtgtta ccacgagatt atactagaag aaatgaaatc
     1141 gagctggtgg taaaagaaat ggcggaacaa gtagccattc gtatcaggca gcataattgt
     1201 aaaacaggat gtgttcattt aaacattggt acgtcgatct tagaaacgag accaggattt
     1261 tcacaccaga tgaaaatacc tatcacagat aatacaaagg aattgcagaa ttattgtctt
     1321 ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat aacctatagc
     1381 aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca aaagcaaatt
     1441 aacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg ttttacgtcg
     1501 attgtacatg cttcttcaat gttagaaagc gctagaagca ttactagaag cacacttgtt
     1561 ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg atcgtggacg
     1621 tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg ccgacaatga
     1681 acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga ttgcggaagt
     1741 attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag aacgaaacgc
     1801 tgaaggatac tatccagaag atattgttgg caagatctta ggtagtgatg agctggggat
     1861 ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat tttaccagga
     1921 taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa gtatataacc
     1981 tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa tgaggttggt
     2041 gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt taggttttat
     2101 attttcccgt tatatatata aggtatttta cggattctat agagttatct ctatgaatcc
     2161 ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa actattattg
     2221 caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac atttgtttac
     2281 tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga acaattttgt
     2341 gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca acgtagaata
     2401 aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt aaacatgtct
     2461 gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa gattaaacat
     2521 gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat tacgatatct
     2581 taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa gaagggaatt
     2641 attagtaaca atatagacag tttaataatg tttaaattat tctagttgct gctcgcaaaa
     2701 catatttctt atgattaata aaaaatttta aagtaataaa aagtgattat tttactttcg
     2761 aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac aaaataatca
     2821 cacaatatta caatataatg catatacgat attgcaatat cacaatatta tgatataatt
     2881 atattataat ataagatgaa tgggggatat tttaatggca agaatattgt cagtagccaa
     2941 tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat gtttgaaatt
     3001 tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg ctacagataa
     3061 tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat taaaagggga
     3121 ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc cagccgacat
     3181 agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca ggcttaaaag
     3241 agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc caccaagttt
     3301 aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc cagttgaagc
     3361 tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa cggtaaaaga
     3421 atatacgaac gacaagcttg aagtacgagg agtcctattt actaagtata ataagcgtta
     3481 taacatttct aaagaaatga cgactgcagc aaccgaaatt tctagagtca ttggtgcaaa
     3541 aattttcaaa acttatattc gtagaacaat aaccgttgac gaagcgcaag ctgctggatc
     3601 agatctaatc aattttaata aaccctcaac agctgaagat gactataaag catttacaga
     3661 agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt caagctgcag
     3721 taagtagact actaggaaat aaaaataaaa agaactacaa accaaaagaa gtagaagaaa
     3781 tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt agtgtaagaa
     3841 cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat tggacgacta
     3901 ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta aaatccatac
     3961 ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa atttaattta
     4021 cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc agtagtatca
     4081 aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata taatgttatt
     4141 gtaatatcgt gatatagtta ccatgagctt aaaccctgaa atagagcggt ttgctttaat
     4201 ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa caaagtttgg
     4261 cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa ttcattgtaa
     4321 catagttttg aacaaaacgc tatcctacag aaagaggatt ggctatgaat gattttaatt
     4381 attacaaatc aaaagaaatt tatcgtgaaa aatattatca aatgcctaag gtatttttta
     4441 ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat tgcttatatg ctacttaaag
     4501 atcgatttga ttattcagtc aaaaataact gggtagattc tgatgataat atattcttca
     4561 tattcacagt tgaagaatta atgaaattgt tacaatgccg cgaaggtaaa gtatctaaga
     4621 tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa gagaggaaga gtaaataaac
     4681 gtgatggtaa gattgaatct atgcctaatt tattgtactt aggcaagcca gaagttacga
     4741 atcaagacgt ctttaaaatc atggaagagg aagacaatac tgattccacc gtaattgcga
     4801 aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc taccgtaatt gcgaaaatcg
     4861 caaatacggc aaaatccagt catatcaagg actcaaccgt aattgcgaaa atcgcagaca
     4921 atctattcta ttctaattct atagacacta atagacacat aatagacact gaaactgacc
     4981 agttacaaaa tcaagttttg ctagataact tcgtcgacat catgcaagag gacagtataa
     5041 atacatttgt accagaaaat gtattaaact tgataaaaac attttcttca agttacagtg
     5101 aagctcaaca aaccgtccaa actattcata atgcaaagaa aaaagctgaa gaattagagc
     5161 aaactattgt cgtttatgaa gaactggaat cttatggaat tgatgctgac aaaggattat
     5221 atatgacttt actgaaagct tatcaaaaac aaaaaactga aaaagtaaac aatttgcaga
     5281 atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa agctattgcg gcaaagcttg
     5341 caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa aattggttag
     5401 agtagttgat aaaaagcaat tactataaaa atgatcaatt ttaaattaga acttttttat
     5461 gattttaatt ttattacaaa tattacaaat aaatagttag tattccggag tttttagatt
     5521 aaaagtttat gaaaacaatc acaatcgtag gttttaaatt cataatcgtt agatatttta
     5581 gttatttttc actcaaaaaa atagggaact agtgtttgca tatgtgcttt tttgagttat
     5641 aataattcat gaagagaggt aattcactta tttccccaag ctgaaaagcc tgatattact
     5701 tcttttcagg cagtgctgct aatattcaat tacacttttt tgtatatttt tagttgtttt
     5761 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggacgat
     5821 aaatttggac aattgaaaat acaaaaaaaa gaaaggccct gtgagactcg gaaggctggt
     5881 aaccttcgtc gacgtccaca ggtggcatta agatcactcc cttaatgcgt ttacctttca
     5941 gtgttacggt attacccgta acggatttag tataacagat attcaaaagt gataccactt
     6001 tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcata
     6061 atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa
     6121 cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt
     6181 tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa gtaagtgggt ggattatttt
     6241 atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa acggtgtacc
     6301 gctacagaaa agatcacgaa gaaactatca actggtcaaa gtaaaaccgt tgtgcagtgt
     6361 aagaaatata taatccagaa agacaaagtc tcagaagaaa tgatttatta cattgggaaa
     6421 caaaaacaaa taattttaaa agatcctatc cctttaaaag agctatatcc aacaatcaaa
     6481 catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt
     6541 accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa cttgtatttt
     6601 atcgtttttc gcttgcttga aaatattgtc gagacttcta aaggaactta gaatcactag
     6661 aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac ggggtaggtt
     6721 tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt tggatagtgg
     6781 tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt agaagttaga
     6841 caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta ggctttgacg
     6901 caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg
     6961 tcgtgtgctg cagtacatgt agatacttgc gaagacgttg atctttggat caatcgagca
     7021 tttaccgttt tctactaggg tttctcgttt cggacgccat aaacgagtcg atttatttta
     7081 tatatttttg agtgaacgaa aaacgataaa aatattcgtt atgaagtaaa actaatttag
     7141 tagatagcaa aggtggtaga ggaatgattc aaaaatttgc gaaaatgagt aaaactgcat
     7201 ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt agctccaaag aaattcaaac
     7261 gagagattgc agaaaaagaa gcaattttgt cggtattgaa tgcggtagac gataaatgtc
     7321 cagtaaaaat tagtttttca gatatagcaa caattatcga aggtatttat tatggtgaag
     7381 ttgatgaaga gatatcttct acaaaagatt tattagattt tttggataaa aacaaagata
     7441 cctttatagt actaactgat tatgaggagg ttacaacaaa tgaaaaatta tgatccgaat
     7501 agccgatggg gaacacatac gattaaggtt tctttccaac gttgggatta caaaggattt
     7561 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     7621 gatttatacg atcaaaaatt gactgataat ccaattggat tcggcttgct acctgaagat
     7681 gatcaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     7741 gttgaagata cttggtcata tttgagtgat tatattgtca gcgttgaaat cattgaattt
     7801 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     7861 gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag gaatttattt
     7921 aggaggaaaa ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa
     7981 aaggaaggta tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaatccc
     8041 tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa
     8101 cccataatgg aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga
     8161 taacccaaga ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa
     8221 caaggaaggt aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga
     8281 aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg
     8341 tcatgcaata tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt
     8401 caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac
     8461 tattgttaga agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg
     8521 aaatgagaaa aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta
     8581 tttggttttt taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac
     8641 caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa
     8701 atcaatgaac atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct
     8761 ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc
     8821 tttgcacaaa ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat
     8881 caatacactt tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa
     8941 ggaaactatg tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt
     9001 caaaagttgt cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac
     9061 aaggtaacgc gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa
     9121 gagaactctt tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact
     9181 tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa
     9241 ggttttaatc aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa
     9301 tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt
     9361 tcaggggtat caagtacacg cagaagatgt cacgcaaaaa acaccaccag agaaggtcaa
     9421 tatcacggtt cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg
     9481 gatcaaaaac gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa
     9541 ggcagattat ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag
     9601 agaagattct gatctaacca acgcgaaagt ggacgagatt gtacaagatg tagaagacaa
     9661 gggatctaac agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga
     9721 aaatggtgag atcactttta ctgatcaacc agcctatgtc aattccaaag ggaatgtata
     9781 tgtggtttat gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt
     9841 tgtgattgcg ccaatgacag ataaaacagg atcaggattt ttaaaaaata ttcatttgta
     9901 tcctaagaac attgtttcta agttgagttt tgaattaacg aaatttggtg acgatggcgc
     9961 agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt gagttataca agggcgaacc
    10021 tggaaaagga acaaaattgg gtgatcttgt atcggatgat caaggaaagc tgactgcgac
    10081 tgatttgact ttaggaaaat actattttgt ggaagttcct tcggaagttg tagtgggatc
    10141 agatcaagaa ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcgca
    10201 taataaatta acctttgaaa ttaccaatga cggcgtgaca agtgacttaa aagcttccta
    10261 cgtgaactat aaagcaccag tgatcgataa aacagtgaca aatggaacag gacaagaaca
    10321 ttctttccaa attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat
    10381 tgcaggtgga acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag
    10441 cgtatttaag tggggagata cagcaggaca aggactttct tatgtcgctg ctaaagcaaa
    10501 tatcaaagta acaaataaag atggatctgt ggtattaaaa gaaaatacag attataaaat
    10561 ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga
    10621 tactgtagca agtttacacg gacaagatct tcaaatgact tataacatgt atgtaaatga
    10681 cagcgcagct gttgccaatc cattaacaaa tagcgtggat ttcgtttata acaacaatcc
    10741 atttaatcag gaagaacacc acgaaaaaac gaaagctgac gttgtgactt atggtgcaaa
    10801 attcttaaaa gtcgatagcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg
    10861 cgcagaattt gctgctaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga
    10921 taaagacggt gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa
    10981 atcagataaa gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga
    11041 agaaacaaaa gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa
    11101 caaaaactca tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat
    11161 tccattaaca ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg
    11221 agcaggggca ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat
    11281 gattattcgt aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt
    11341 gctgtcggca gtagcagtct atttaattcc agctgtggaa acaaatgtag cgaaatttca
    11401 acaacagcaa agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt
    11461 tgcgaaaata gagttaggcg atcctgttgg tgttcttaca attccaagta ttcctttaaa
    11521 actaccaatt tatgacggaa cttccgacaa aattttagag aatggtgtag ggattacgga
    11581 aggaactggc gatattacag gaggaaacgg taaaaatcct ttaatcgcgg ggcacagtgg
    11641 actttataaa gataatcttt tcgatgattt gccttcagta aaaaaagggc aaaaattctt
    11701 tattaaggta aatgaagaac aacatgccta tcaaatttat cgaattgaag aagttaaagc
    11761 agaagaatta caaaaaaatt atttagatta tctcatgcct gaaaaagacc aagataggat
    11821 cacgttaatg acatgcacgc caaaaggtgt taactcacac cgctttttag tgtatggtga
    11881 acgagtgtca ttcagtaaag aagatttaaa actacagaaa aaagcaaaaa aataggaaat
    11941 caatttcatt ggattggcga ttagcactgc tattcgcttt ttttgcgttc ctattatttt
    12001 tatttataaa aaaataagga ggagatcgga atgaataaga gaaaatggct gtttgtttgt
    12061 gtagcagctc tctttgcaac tgcagcttac tcacagatat cttttgctga tagcagtact
    12121 actccaacta ccagtcagac aacagatagt tcaacaactg caacaagtga cacggacagt
    12181 tcgaagaaca ctgcagaaga aacggcagat tcaagtacga gttctactga atcagcggaa
    12241 tcaacgtcag aaagttcttc agagacttca gaaaaactgc aagaagtaga agatacgcaa
    12301 gtaatcaagc aaaacattca tattcaaaaa attatttctt cgaatcgaat caacaatcaa
    12361 ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa agtttattgt ctacgatatc
    12421 acgaatatct tagaagaaat gacacaagat gataatgaga aaaatcaatc ggtagaaata
    12481 attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgatcagtt gaaaaaagtg
    12541 actgaaggag aaacccaaac aattgatgat caggaaggcg tgattgattt ttcaattgaa
    12601 gtacctgcaa acaaaaaaca agcatactat atcgtcaatg aatcaagccc tgaaaacatt
    12661 tcaaatagcg aagatattgt tttattgaca cctgtttcag atgaaaaggg tgaatttttg
    12721 acggatgttt ggttatatcc taaatcaaag aaatcagagc cgaaagaaga aattaaaaaa
    12781 gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt gttgggattt tttcactcat
    12841 ttatttttta acgattagga tgaaatgatg aaaataataa aaatgttagt ttttaatcta
    12901 tcttttttag ccgcaacaat attgtgttgc ggtttagttt tttctcaatt atcaatttac
    12961 ccaagatatc aacgattgtt cgttcatttt ctaaatatcc agcatgcttc catgccacta
    13021 gaagtgatta acaagctctt aatcaatcca atttttatgg ctagtttgtt tcttcttgca
    13081 ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag cttacaatcg tttaacaaaa
    13141 taatagatgg agggcattat ggaaaataaa attcattgca ctacgggaag agtatttact
    13201 ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa ctgcagtcca ggccttagaa
    13261 aaagttgatc cgagtcaaat caaagaaaat aataatctgg tactttatca tgatagtgat
    13321 tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga tgagtattca
    13381 actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag cacgtcaaaa
    13441 attgttgccc attatcaaaa ggtgggcact tatctaggaa aagaaattga ttgcgacgta
    13501 actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt
    13561 aatgggaaaa agtattataa ttatttgctc tttgataaaa acatttatcg aggctttttc
    13621 atgcagtcaa tcaacgttat gaatgttaaa ctagatttta agtatgcagg atcaaaaaca
    13681 aaagtagctt tgagtgaacc acagccagaa attaattcaa aaatgagatc gatgagagca
    13741 gctggtattg agttgaatga tacatttcta tctatttaca gtttaaatgg acagtatcag
    13801 caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg
    13861 agagaatttg ttgggtacga aaaaatggat cgcttacctt attttgtcac aacagataca
    13921 gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg
    13981 ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct
    14041 gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg
    14101 attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct ttggacacca
    14161 caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac
    14221 aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag
    14281 tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc
    14341 tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg
    14401 tatgttgaag cgaatgggac ttggaaaaaa gctgatacag gaggaaaatt aactgtatca
    14461 ggtaatacag tcacttatac tgcttctgac agctttttag ctaatgctgc aaattacggt
    14521 agggactaca ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca
    14581 aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataaa
    14641 gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt
    14701 gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa
    14761 gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc gctcgatcat
    14821 tacaagcaat ttagtattca agatccagtg gaagctcgtt tgacgtataa agaaggttct
    14881 gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtat actaacctat
    14941 gatagccatt caagaacgct gaaatgggaa gcttccgctg atttcttaaa taacaatctt
    15001 ttagatggac gagaaattca actgatattt acagctaaaa ctcctttgaa tgaagaattg
    15061 gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta
    15121 acgattgggg taaaacctaa cttacctcag gtcattgtgc caaaaactgg atcgaatcat
    15181 ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta
    15241 gttttaaaac tcaaataaat ataaaaacaa ggctgatttt cagccttgtt ttcttttatt
    15301 tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa
    15361 ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat
    15421 gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt
    15481 catcaatata cagcccagtt tgacttatgt tttctccctt agcaccatag cagcctaagc
    15541 tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta
    15601 attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa
    15661 caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac
    15721 gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt
    15781 tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca
    15841 agaaataatt ttgatcagtc atatttttcc aaggagtaga gaagctatcc ccttcgtata
    15901 ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca
    15961 cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg
    16021 ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag
    16081 ttaaagtgac actttcaccg tgagtgacgt tgattatctg ttctttcaaa atatcaactc
    16141 gcccctcatt tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat
    16201 tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt
    16261 tgtagctaaa attatcttta gcaccttcgc tatatttaaa tgcttttgcc ttagcagtgt
    16321 aagtagaacc agaagtcact tttgcttgat ctgtagcaat cacttgacct gtttgattgt
    16381 caatatggtt aatggtaacc atatattctt ttggcgtttc aggtttgtgt tgcaaccctt
    16441 taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat
    16501 cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg
    16561 taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat
    16621 tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg
    16681 cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta
    16741 aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa
    16801 cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc
    16861 cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt
    16921 tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga
    16981 tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat
    17041 ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt
    17101 tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt
    17161 cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa
    17221 aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa
    17281 cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat
    17341 taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat gattattata
    17401 tatcggttaa ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt
    17461 cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa
    17521 tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact
    17581 tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc
    17641 aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt
    17701 ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag
    17761 aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt
    17821 tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa
    17881 ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat
    17941 aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa
    18001 gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg ggaatattct
    18061 gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa
    18121 ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct
    18181 gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt
    18241 ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcacaatca
    18301 aataaataca aattacaaaa gactagcgag tgattgctag tctttttatt tgtcctgaag
    18361 gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa agccgcgtct aaaatcttag
    18421 aacgccgcgg tgtatctgaa acacagtgga aacaagaagt ccttgaaaaa gcacaatttg
    18481 acctattacg gggaaaagac aaggacttag aaggatatat ctatcaacgt gaacgtgaaa
    18541 agcttgtttt cgatgaaatt attaaacact aaaaggagcg aacaatttta tgtttgaatc
    18601 aattacaatg aattttttaa cacaaccgaa agtagtagct gcggttgata tttttagagc
    18661 attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgctg ctgttgcggc
    18721 agtggcttcc attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa
    18781 cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc
    18841 tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaacaa
    18901 gtagaaggag taaaaagtac gtggctactt tttactcctt tttttatgga aggagaatat
    18961 tcatgttttt tacgatttca ggcaatgcaa aattggataa agagttaata agaaaagcaa
    19021 cgaagaaagt gggaaagcgt acttcctttt taccgaaaac aattaaatat tctgtcattg
    19081 aaacggaaga acagctacat gtgttaggac aagtgttaga acaagagaat caatcttctg
    19141 ttaaattgac tctcacgtta catgataaag aaggaaaaat agaaaaggct ggcacagaat
    19201 acgtttttgg ctcactggat tatcaaattt cagaaggttt ttatcctaca ttgattgatt
    19261 taatccgcct agatatattt aaagatacgg atgaaaccat gagtgtggaa gagtcttatc
    19321 gaaacgaaaa aattttacaa gttttagaag aagagttaga aagcgaccaa agaaaagtga
    19381 atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg ggaacgccca atctttccaa
    19441 aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga acaagctgac attttgactc
    19501 ataaatcaat atttgaggaa gagaacgagg taatgggtca attggaggta gatccctttg
    19561 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    19621 aagacccaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    19681 aagtagagaa aaatgaaaca gagatacaaa aggcaacggt atttgattat attttggatc
    19741 aatatccgcc agagcatgaa tggatgaaag aaaaaaataa attattttta aatgaacttt
    19801 attcgaaata tcaattacct caaacctatc aagcattttt agagagtaaa gaagaattaa
    19861 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    19921 atgagcttgc acaagaagat ttattagcta cttttgaaga acgtcaacaa gaaacagatc
    19981 aagaaattaa tgagtatgcg acggaacagt cgtcgaattg ggagaaagca aaagaggaac
    20041 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcggtacg
    20101 aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa gatcaacaat tttactcaga
    20161 cgaatcaaga agcattatcg aaagaaaaag aaaacttatt aaaagaacgt ataaaagaac
    20221 gaaaagaaga attttaccga gggttgcgta ctgataagct aaggattagc aaagaattaa
    20281 atgaacaact gaataaactc tatcaaacta cgactgaagt tctgatggaa aaacacgagc
    20341 agatccaaca agaattacaa gcacaaattc caaaatggaa aaaagagtat gaaattcacc
    20401 aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa agaaaaagag cgcgaacaat
    20461 tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa aatggcgata gaaaaacaac
    20521 ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    20581 atacattgcg acaagcacag ttaaattatc tgacacatcc tcaaccagta gctgctgttc
    20641 aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc ccagcgttcc tttaacggat
    20701 ggatggtggg atgtattgca agtttaagct tgcttctagg tggaggaaca gtatttgctt
    20761 ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg tcaatcaagg
    20821 aagaagccca ggaacaagca agaaaagagt atgaacaaca attagaagaa ttattgaatc
    20881 gtaccaatca gtcaacaaca gagtcaagca tggaaaatag caaagagagt gattctagta
    20941 aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa agttcagaat
    21001 caaatgaggt gaaataatgc gtgttagaga aacaacgaag cgaaaagaca aacgattcaa
    21061 tttagaaaag aataggtatc tttctcattt ctatcgctat actccctcag gagaagagct
    21121 accagaaaaa catgacaaac gcatttcgaa aaagattttt tatagtggac ttgtatacct
    21181 ccctttagag tatttagtaa caagagtacc agaagttttc aaccgattgg aaattgttaa
    21241 gggaagctta caaggaataa atctgtttca attacaggaa atgccacagg tctttcattt
    21301 tgattcagct ggtttttgga atccatttac tcctacaact agtttgggta cacgtttagc
    21361 aatttcgaca gtttgtgtgg ttccagctgt acttatggga ttgaaaatgg acatgtggtt
    21421 tcgtcctcta gcagatggac aaaaagggga taatcgatta atgaccttaa aagaaatcca
    21481 acagatttat ccgcaaatac ctgaaacaaa aactactttt ccaggttatg gaggtattcc
    21541 aatcacacat tacaaaaaat attggtacat tcaaacagat actgttaata cgtgtattgt
    21601 tggtacctct cgatcaggga aaggtcagat tgaagtgtta gccaccattg acaatttatc
    21661 tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca aaaacagaat tatatgttgg
    21721 tagtaaagat gaactagaag ctagagggta tgatgtatat gcctttaata tcaccgatcc
    21781 actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga aacgaggaga
    21841 catagatact gcaacacagt taaccaatac tttcacaaat acgatttatt atgatgccaa
    21901 cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa gctgtcaatg cgcttatttt
    21961 tgcgttgtta gaacaagctg atcaaacagg agattactcc aaattgacac cgaataatct
    22021 cgttgagctt ctttctgaga ttggcggttt taattattat ccagatccag acaatgaatt
    22081 caaacagttg aatgccttag atgaatttat gaatcagttg ccacctggaa acattgcaaa
    22141 aaaacaatat ggggcaacga aaattggatc tgataaagcg aagggaaata ttttatcaac
    22201 ggcaatcacg ggattgaatc catttactct aacgaaaatt gcaaagatga cttctcaatc
    22261 aacgattgat tataaatcgg ttgggtttcc aaaatactta gatttgaaat tacatgaaag
    22321 tcttttaaat caacgagtcg tagtagaatt ttatcataag ggaaagaaat ttcatgaaga
    22381 acgtgtcaaa gtaggctata aaggatttag tgaaatcaat tttgattgtc atttagttgc
    22441 aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa gaatatgtgg cttggtatga
    22501 attttctcaa agagtattga aagacgaaga gggtaatatc gtttatcgga aaaaacgagg
    22561 agaaacccat ttgccagaat tagaaaaaga agccgttcta acattagatg aggaaaaaag
    22621 taacttatta gtagagtcta ttcggatgca ctataccgat aaaccaatgg ctatttttat
    22681 gcttacacct gattatgatc catctaacca tatcattgtt agtatttttc ttagtcagtt
    22741 atataaagaa ttatctacgc aatgtgttaa gactaaagga gataaatgtc accgcaggat
    22801 tcattttatt ttagatgaat ttgggaatag tgtgacctgt ttagcctaag gataaaagag
    22861 gctagttcca acgaacattg gaataacttt actatcgaga aatcaaatga cggggtaacg
    22921 ccctgaaggg tttccactga tactgcgaca tgcgttagta ggatgtgcat ccaaacgcgt
    22981 atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa agacacgata cgtatctacc
    23041 acgcaagtgc tctagaggta gggtatgtac ctgatatgtc gggtgtccta aaaagttcaa
    23101 taaggtgaga atgatttgat tatctgacaa acttgcgaat ggaatgtctg aaacgaataa
    23161 ctcatagaaa tgtgaggggg aagggggata cgtcctcttt ccattgactc gaggttgagt
    23221 aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc tatggagtag tcaacaggca
    23281 aaagcaagca cctaagttga aatctatgga tagatagtaa ggaacagggg aagcatctag
    23341 cacggaggga gcataccgaa gatgtgtttc taaggaaaaa ccgaaatcgg ttataactta
    23401 ggtgaataga tgtgaaagta gagccacgac cagtgaaaat ttcgtaatga aattggagga
    23461 acaggctcaa gtcgttattt ataatataag aaacctattt aaaatcatgg attctcgtaa
    23521 gactaaaaag gtcacgaacc agaatggagc gtgataaaac catgactaac acaaaaggaa
    23581 ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta cgacatgact gagactttcg
    23641 atgaattgca cgcaatgagt gttgcaaaat caaattttaa aaatctcatg aaattcatta
    23701 ttgatgacaa taatatcttt ctagcgtata gaaatatcaa aaagaataaa ggaagtgcaa
    23761 cggccggata cgatggtgtc actataaaag acattgaaaa attggcatgt ggtgactttt
    23821 taactattat taaaaggcga tttaaggatt acagaccaag aaaagttaaa agagtagaga
    23881 tcccgaaacc aaacggaaaa cttcgacctt taggaattcc ttcaatatgg gacaggatag
    23941 tccagcaatg tattctacaa attttagaac caatatgcga agcaaaattt aataagcata
    24001 gctatggatt cagaccaaat tgttctacag aaaacgccat agcagattgt cttgctagaa
    24061 tcaatcaaag taagatgaat tatgttgtag atattgatat aaaaggattc tttgacgaag
    24121 taaaccatac caaacttatg cgtcaaatat ggacaatggg gattcgagat aaacaactcc
    24181 ttgtaattat tcgtaagatg ttaaaggctc cgattgttat gcgaaatggc aatgttgttt
    24241 ttccaaccaa gggtactcca caaggaggga ttttaagtcc actgcttgcg aatatatgcc
    24301 tgaatgaatt tgattggtgg atagctaatc aatgggagga acgaactgtc aaagagctga
    24361 aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa tatctattcg aaattaagaa
    24421 gccacacaag tttaaaagag atgtatctgg tacgctatgc agatgatttt aaaattttct
    24481 gccgaacagg agaagaagcc caaagggctt tttatgccac acaaaaatgg ctcagagaaa
    24541 gattaaagct cccaatttca atggagaaat ctaaagttac gaacctaaat aaagctgaaa
    24601 gtgaattctt gggtttttca ataagactcc acaaaaaggg aaaaaagaaa gtggctttca
    24661 cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa actaaaacaa cagattaaaa
    24721 aaattcaata cgctggagca aaaaaaaata ggcaccgagc aataggtgac tacaacatga
    24781 tggtcatcgg tatccacaat tactatcgga tagcttcaca ttgtagtgtc aatttcactg
    24841 agatttatag aagtgttagg acagtaatag aagcaagact gaaagctaga ggaatgacga
    24901 aaaatggaat ctatggacaa gatcatgaag gataccgtcc gtatctaaag tctaagcaaa
    24961 tgagatatat tgaaggtttt ccgatacttc caattggcta tattaaaaaa agaactccac
    25021 tcaacaagcg gagaaatcta aataaatata ccaaggaagg gagaaagctc attcatcaag
    25081 agcaacaagc tatagaaagc tggaaaatca agtatcttgt aaaaggtttt agtaacaata
    25141 agaaatacac ggtggaattt tctgataaca aaatatctaa gtttatttcc caaaaaggga
    25201 aatgcgctat cacaggaaac gaacttttga tggaagaaat tgaatgccat cacattaacc
    25261 catatcattt gtccaaagac gatagttaca agaaccttgt cattatctct agagaaatcc
    25321 atagattaat ccatgcaact agtgaagaaa ctattctgaa actgcttcgg ttactgaaac
    25381 taaatcacta tcaaattaga aaactaaaca cgtatcgaaa aaaagcagct aattttccaa
    25441 tatcaatcaa aatgaattag ataggaaaca aattatattg taattaacga tggaacgccg
    25501 tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa aagacggaga taatatcaaa
    25561 ctcttaccta tcggaattgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    25621 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    25681 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    25741 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    25801 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    25861 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    25921 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    25981 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    26041 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    26101 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    26161 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    26221 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    26281 tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttgggaaacg
    26341 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    26401 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    26461 aaagtcttac aagattatgg taattatctg cagtatcaag atgtgttcag ttggatttgt
    26521 aatacttttt tatgggggtt gatcaaaggg ttatataatc tcaatacttt actagaaaaa
    26581 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgaacta
    26641 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    26701 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    26761 tttatggcca tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc
    26821 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    26881 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    26941 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    27001 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    27061 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgactggaa
    27121 ctggatgata atggaaaaga aattcctgtg caaatcagtg atgaaggctt agccaaatat
    27181 gtttatactt cagggtatcg tcgatacatt agtagtccag ggattatttt agctggtgaa
    27241 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    27301 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggacaa
    27361 cggatgaaaa gtgctatcga agatatttct caatccttct tgttattggc atttacaatt
    27421 ttagaattac gaatctatac cttaatgctt tcaggtattg gtgatttgca tgcggcagga
    27481 aagatcaatg gattcttgta tgttgtcgct ttgattgtac taaccattgc gctattcaaa
    27541 ggctctcaat cagttacgaa aattttcggc gttgatacga gtctgaaaaa cggcagtaat
    27601 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    27661 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    27721 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    27781 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    27841 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    27901 gaagatctta cagaaaaagc caaagacacc ttagaagata aattagacgg cacgagagct
    27961 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    28021 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt caatgcagca
    28081 gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    28141 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    28201 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    28261 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    28321 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    28381 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    28441 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    28501 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    28561 gatagtcgtt tgaatcaatt caaggataat gatcaaagtt ttgttcatca atcacctgca
    28621 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    28681 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    28741 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    28801 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    28861 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    28921 ggccttttct ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    28981 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    29041 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    29101 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    29161 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    29221 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    29281 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    29341 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    29401 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    29461 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    29521 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    29581 aatcgtgcaa aagcggcagt tattagataa aaatcgtatg gaacagtcct taggaagaaa
    29641 tctacagcga ttaagaaaat atgaaaagaa attagtagag gaaatccagg aattgaaacg
    29701 agcagaacag gaacgccctg atttaagctt ctttttcgtc attgaaggaa agacaatcaa
    29761 ggatttgcaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    29821 actctacaaa aaagaattga tcaagattct ttatcgaatg aataatatga atgatgagta
    29881 ggaatgggga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaaag
    29941 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaaggaaaat
    30001 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    30061 aacagctttt tcaccacaaa atgatcaaaa ggtacttgat ccattaaagc aactaggaaa
    30121 tcgggaaata gaaattttaa aatccaaagg gtatgattta gactttttaa aacaagtgat
    30181 gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc tatggaaatg gttattcacg
    30241 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    30301 gaacaatcct catacagtgg caacactaga tgttagcaca gatgaaaagc aaaaagtgat
    30361 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg tcagataatg aacgaaaagc
    30421 gactgaccga aataaaagcg cggaagaata cctagaacta gcggaatatg cccgaaaatt
    30481 gacttctaat ggggaaattt ctaaacaagt caaagtgaga gtacatgttt acggaaacac
    30541 gattcaagaa gtagatgaac gagcacagga actgatcgaa aatctcaaag ggatggatta
    30601 tcgtgcagtg acctatctgc acaaaacgaa aagagactgg caatcgatgt tcaccatgtt
    30661 cgatgaacaa gctgaatggt ttggaaatat caaagggcaa tcactaccat cgctaaacat
    30721 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggtggaa cacaatatgg
    30781 ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg tataaaaata gtgtgcggtt
    30841 gtctaccaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    30901 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    30961 ctttattccc tttttaaagg aatataacgg cattatggtg cgtttagacg gatctgacgg
    31021 gatgatcaat cctttagaaa tatttgccac acgtacgtat gatgaagctt ccagtttata
    31081 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacgcactt
    31141 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    31201 agaatttaag acctatttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    31261 tacttcctca aattcgcgtt caaatactga tacgcaagtc acaggtaaag atccagaagc
    31321 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    31381 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    31441 gtatggcatg atttttgatg gtcatagtac gattcgacac cttgaccaac aacagttggt
    31501 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    31561 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgacgttt
    31621 aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc ttcttggcga tgattgatga
    31681 gtgccataat ggtgcgacct gttctgcagt gaaaagctgt gggttgcaag tagtctaaaa
    31741 actattgtaa taactcttat aacgagaagt caaatgagtg ggtaacgcca caaagggctt
    31801 ccactgatac tccgacatgc aacaagtagt ttatgcagac caacgttgtg tgaagctcgg
    31861 tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt gaataccacg aaagttctct
    31921 agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa aacttaatat ggtgagtatg
    31981 tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa ggcatagctc ctagaaatgg
    32041 gagagacagt gagcaaaaaa tcactcattg tgctaacttg tggttgagta aaagtcgttg
    32101 ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt agggggcaaa agctagcatc
    32161 taagttaagg catgtggata gatataaggg aacaggggaa gccatcaaca tggagagtgc
    32221 atactcgatg aagtggtagt aagaaatatg tgaatgacaa ataacttact ttagtgatgg
    32281 tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa ctggaggaac aggctcgatt
    32341 cgtctgtaaa agtcatgaaa cccgttcaac catgggattc tcgtatgact aaaaaggtca
    32401 cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca agtccaaaag ataggaagtt
    32461 gcggcatgca gaatattatg gcatgacgga aatcttcgat aagatatact ccaaaagttt
    32521 gaagggagat aatttcaaga atctaatgca gattatcatt tctgataaca acattgtcct
    32581 agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca agaggagaag atggagtaac
    32641 gattaaagat attgaaaatc tcaaccgtaa tgaattcgta gaaattgtta ggaagcgatt
    32701 cgcacattat aaccctcgga aagtaaggcg tgtagaaatt cctaaatata atggtaaatt
    32761 aagaccgttg ggtattccat caatatggga tagaatcgcg cagcaatgca tcttacaagt
    32821 attagaacca atatgtgaag caaaattcaa taatcacagc tatggtttcc gcccaaatag
    32881 atcagcagaa cacgcaatag cagattgtat ggcccgaatg aatcaaagtc acatggaata
    32941 tgtggtagat gtagatatca aaggtttctt cgacgaagtc aatcatacta aactcatgag
    33001 acaaatctgg tctatgggta tacgagacaa acagcttctc ttaatcatta ggaaaatgtt
    33061 aagagcaccc gtagtacttc caaatgggaa gttgatatac ccaacaaaag gaacaccaca
    33121 aggcggaata cttagcccat tacttgcgaa catcaatctc aacgaatttg actggtggat
    33181 agcaaatcag tgggaggaaa aagatgccaa agagctaaaa catcatttta ggaaaaatgg
    33241 agcttgggat aaaggtaata tctatcacaa gctcagaaag agtacaactc taaaagaaat
    33301 atacctagtg agatacgcag atgattttaa aattttctgt agaaattata atgaagcgaa
    33361 aaaagttttc tatgcaacta aaatgtggtt acaggagcgt ctaaagttac caatatctga
    33421 agaaaagtca aaaatcacaa atctttcaaa agaagaaagt gaatttttag gctttacgat
    33481 gaaattagtg accaaaggta acaaaaaggt tgttaatacg catataggta caaaagcaca
    33541 aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga attcaaaaaa gtcctaatag
    33601 caaagatacc tttatctata ttggagtcta taacagtatg gttattggga ttcataatta
    33661 cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa attgcaagag aactcagacc
    33721 aagttttgaa atacgactac gagaacaagg aatctcaaaa gaaggacaat acggaaagta
    33781 cgaaggatac aagccttacc tacggtcagg acaaatacga tatataagag ggtttccaat
    33841 attaccagta gggtatattc aacacagaca tccgatgtgt aaaaaaagaa atattaataa
    33901 atacaccgaa gaaggtaggg aagccattca taagaagcaa caaaccgtag aagaatggaa
    33961 gatatcgtgg ttgtgtagca atccagtgct tagtaagaga gcaacgattg aatttgcgga
    34021 caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt gcagtaacag gtgaggagct
    34081 tatcttatct gaaatgcatt gtcatcatat tatcccttat catgaaagca aaagtgatag
    34141 ctatgaaaac ctagtgattg ttactgagga ggttcatcgg gtaatacacg ctacacaaag
    34201 tgaaacgatt gaggaacttc taaaatatct aaaattaaat ccaaaacaaa aagaaaaact
    34261 caatgagttg agattaaaag ttggtaacga agaaatatct taataaatat ttgtaggacg
    34321 ggaaaacaga atgtctttta tagacgaggg aacgccgtat gcggtgaaag tcgcacgtac
    34381 ggtgtggact gggggaaaag acggagataa tatcaaactc ttacctatca gtatcgatta
    34441 atccagattt tattgaaacg gtaaattatg tcacaaactt tcaacgagaa atgcgtaaag
    34501 tagaagcaat gactgtttta gctacacaat ccccacaaga aatggtgcca gaaaatatgt
    34561 cttctgataa cttctcgaaa ctgaagaaag tctttgaatt ttcttctacg aagatcttta
    34621 tgcgtatgga tgaatctatt ttacgtcata tagaaggatt agttggtaaa tcaatgacaa
    34681 actcagaatt agaatccatt cctcaacaaa atcaaggaga cgctgtgatc aattttggct
    34741 caaaagaaac cattcgtgtt cattttagtc cgaacaaacg tcaattaaaa ttgtttgatg
    34801 gaggaaaata atggaaaacc aaaatgaaag tttaatcaag cagtatgtta agcgtcgtgc
    34861 aaagcgacgc ttatttttgt ggttgtttgg tacttctgca ggtttgatta ctattttgat
    34921 tactgtgttc gtgacccttt ttctaatact tgcggcgggt tcgattgata attctgattc
    34981 agattcaagt agtggtggcg aagcttttac aggagaatat tcggaaggct taccgattta
    35041 taaagaaatc aaaggaagag gccctttttc tgatgagatt gcgcaatatg ctgtaggtgc
    35101 agcagtgaaa tataaattat taccaagtgt gatcctttcg caatacggtt atgaatcagc
    35161 gtttggtacg tcggcgtccg caagaaatga tctcaactat tttgggatta cttggtttga
    35221 tggttgtctg tttcctaaag gaactgcgcg aggaatcggt ggtattgaag gtggctggta
    35281 catgaagttt cccaactcaa aagcagcctt ctcctattac ggctttatgg tagccacaca
    35341 atccaatttt aatgcatgtg taggaaataa aagccctggc gcaagcttat tgatcttagg
    35401 acgaggtggt tatgccgctg caggaattac tgaagatagt ccgtattata caggttgtat
    35461 gtccattatc acttctaata aattaaccga atatgatgaa ttcgccatca aacattgggg
    35521 tgaaggtggc aataacaacg gcacgatcac tggtgaatgg acgaatccat tccctggcag
    35581 cagtttagat aaaagttcct tcagtggtgg tcaattattt ggaacaaatc caggcggtga
    35641 attccgtccg aatggcttcc atgatggatt agactttggt tcagtcgatc atccaggaag
    35701 tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt ggcaatcctg gtatttcagg
    35761 tttaggtgca tgtgtgatcg tgattaatta cgacggtttg aatatggtat atcaagaatt
    35821 tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat caagtgaaag ttggtcaagt
    35881 aatcgggata cgagatacag cacacttgca tttaggtttc actcgaatgg attggcgtca
    35941 agcacaagga cacgcattca cagacgatgg tacatggatt gatccattac catctttgaa
    36001 tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat aaaactttaa aaatcgtctt
    36061 aacagctgca attagcgtta gtttaatcgg caatatcgcg ttgttttggc agctgaaaga
    36121 agaacaagag acaaatacct ttaaaattga agaagtggct aaaaacgcgt ctgagaagct
    36181 caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag ttaaatcaaa ccatcaatga
    36241 gaaaaaccaa gaaatcgatc agctgaagca acaagatggc acaaaagcaa aagaggaagt
    36301 aactgaagca caaagaaaga cggcagaaga gttcggtcaa ttggccattg ataaatcatt
    36361 aagtagaaat gaattaacgg aaaaactaaa agatgtcgca acccaagaag tgatcgacaa
    36421 attaagtcct gcagacgatg atcaccaaca tgatgattac ggaagttact cctttacatt
    36481 aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa gaggaacaaa attttgtcgt
    36541 atttgttgat tatacaatcg gcaatccgca attcaaagat gtcaaaccac aaaaaataaa
    36601 aggtggatta actgtcactg aaaaacaagt agatggtcaa tggaaagtca tagactttag
    36661 ttatttcact cgttaggagg aagaaaatga gtaagaaaaa aggagaaaga caacgtttca
    36721 aaaaacgaaa aagttatctt caatggctgg gaatctttat gattctcggc tttttgattc
    36781 ttgctagtcc gatttatacg aatcagaagg tagatatacc agatgaggta agcacgaacg
    36841 cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga aaaaggagcg aaccagcaat
    36901 tagtttttga atttgcgatc gatgatgaag atcaaagctt gttaaaagaa ctggctaatc
    36961 ttaattatcg cgtagaagta aagacagcta aaggggatta tcaagcaata cgagccaaag
    37021 tgatcaaggt gtctgatgat tattttgcag tgaagctaac gcatgtacca gaagaatata
    37081 tcgccatgcg tttaaccatt attcctgaaa aaattgatcc aaaagtggat atgcaagaac
    37141 cacaagatct gatttattac gtgcacgaag ataaggttaa agatcaagtg aaagatgaag
    37201 attatgaaca acacgcgatc aattacaaag tgaaaggata caagaaagaa caaaaagcag
    37261 ctcaaaaaca aatggaatct ttccaagcaa ccattgattt gaatgaagaa ttagtgaaga
    37321 agctaaaaga tcaattgcct tatcaagtcg cagatgatca ggaaaatact gaaaacaaga
    37381 ttaatagcta ccagcaagaa attgaaacat cgaaaacgca actgaaagat caagaagaga
    37441 tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa agaaaagatt tagaaggaca
    37501 ggtggagtga aaaaatgaag cgaacaaact tggctgacaa agatactttc gctgagcaga
    37561 aagtattgat taaagcctat ctcaaagttg taaaagctaa agaaatatgt cgagaagtga
    37621 atttatctaa cacaacaata tctgagttga agaatggtcg aaagaattta gatactttaa
    37681 aattcagatc atttgaagct ctatatgtag ctgctaaaaa atattttaca gctgataaca
    37741 aataaaaaac aggagtattt tatgtgaaaa aagtatataa aaaatgctta tgaatcagct
    37801 atatcacctt aaccatttgt taatgagatg ggagaaaaca ttaaccacga ttctaacgca
    37861 aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt tcaaccaaac
    37921 aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt tcatatgaca
    37981 aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa tacacaaata
    38041 accttaattt ttaaagagca taaggaaacg tgatatcact ttctcacgaa aggtgttagg
    38101 taggctgtgc ctaccacgtt tctaagaaag ataaatgata tcaaaatttt ggatttgtgt
    38161 gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa aataagcgtt tgtgtgtagc
    38221 aaaatcaaaa aatgtgtagg gatatgtgta gctacacata tccctacaca taataaattt
    38281 ggaggaaaat atggacttat taattagaaa tattgaagaa aagtatatta ataaaattga
    38341 caaacgttgt gaagaactta cggtcaagac tggcaaaaaa tggagtcgaa atcaatacct
    38401 aaaagttttg atcgaaaatg attttgatca cgctcttctg aattataaaa aagaccaatt
    38461 tgatcggttg ttagaaaaat ttgtggatat tcaaacttat aatacgaaaa ctttagaaga
    38521 atatattgca acaaataatc aattaattgg tttgttaatt gaataataaa aatagtattg
    38581 tgaatttata aaaggaggaa atcaattttg gcaaaagcaa caagaaaaca tgtatatctt
    38641 tacgatatgc atatcgaagt acttgatgaa ataatttcta ctcatgaaga tatatctaat
    38701 tatagtgaag cagtcagaca tttgtgtatg atgtatgatc caaaaatcga agaaaaaata
    38761 aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa tttctatttt aaccgaaatg
    38821 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tactaattgg tacaaattca
    38881 actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa ttaaatccaa tcaaaccaaa
    38941 aaatattctt ctaagttaaa gaaaccagtg aatgaatctg ttcataccag agtagatcca
    39001 tttgatccaa cgaagattct aaaaagataa aagtagaagg agaacgtgat gaataaatcg
    39061 cctgcagtta ttcttacttg tcaatttacc ttgcccaata aaaaatcttt ttcaacatac
    39121 attgattata tgacaagaga aaaagcactg gaagaaatgg aacgacgtac gcccgaagaa
    39181 gaacaggaac tgcaacgaat caagaatgct ttagaggatt tttatattcc tgaaggagaa
    39241 actttttcgg aaagtaagac ggataaagaa ttcagtgaaa cgtcgaatga agctcaatcg
    39301 ttgatggaac atggaatcaa ttttgacaag ttagaggaac aagattttac caaatatctt
    39361 tcttatatga cccgtcacta tgcgttagaa caaaagaaag aactgacgca aagggaacaa
    39421 aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc aaacagagcc acataaaaaa
    39481 gcttcaggaa atcttcctgg agttttttct atggcttcag acgaagtgaa aggaagtgat
    39541 ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa aaggctcaat catttatcaa
    39601 gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg gcttgtacga tcccaaaaca
    39661 gataccttag atgaagaagg tttgaaagca gctggaagaa aaatgatgga gacattattc
    39721 gagaaagaac aattgaatga aacaggttac tggatggcaa cgattcaccg taacactaag
    39781 catatccata ttcattttgc agttgttgaa aaagaaaata cgcgtaaacc gtatactgag
    39841 attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga gaagacaagc aacattagac
    39901 gcaatgaaaa ccaattatgt acatgagtta gaacgtttct cttatgaaaa ggatcgaatg
    39961 atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg atttgcgtaa caccttaaca
    40021 aaagtagtaa aagaaccagc acgttacgat cgacacgcca tgaacttatt aaatgaagta
    40081 tataaaaccc tacctgaaaa acgttcggaa tggaattatg gagatgaaaa tcgaaccaag
    40141 ctttcaccat ttacaagggg aaaacttgat gagttaacca aatatgtttt aacaaaagaa
    40201 cctggatatc aagaatatat acagatttca aaagcattga aagaagaagc caaaagcctt
    40261 tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa acgccctctt tgatttaaaa
    40321 aagcgcacag gaaatgcgat tttaacagaa ctgaagagac aggatagtca aataaaacca
    40381 ttaatagaaa gtgtcaatca gatgcaatct atttacaatg aacgatcctt tcgaacactc
    40441 aaacagttgc caacaaatga atacaagtat agttatcaag aattcgtaaa gcgtaggaaa
    40501 caattccaac aaagagttgt cacgcagcga agcttgcgtc aattaaatcg aacaattaat
    40561 gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag agcaacagcg aagaatccaa
    40621 atggaacaag aaagacaata ggaggaagag aaatgaaaca atatttagat caatggaaag
    40681 tcatagaagg atcactaaaa gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag
    40741 agcatttgtt tcagataagc gaaatgttga gaaacgaaca gtttgatcca aatcaatttt
    40801 tagttgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaag ggtgaaaaat
    40861 attttattat tcaagaatac gaaggtaagt tagcgcctta ttatactact tgggaaataa
    40921 atgaagaggg aatcaataat ttcccatgtg aaagcattga agagtctatt tctctcacgg
    40981 aatgttaaat aaatagagac gtaaaagagt cctgaacgtg attgtccagg actctttttc
    41041 aatgaattgt tttccgccaa ttcactgagt ggtgcgattc aattttatta tatcaaaaga
    41101 aagtgagatt ccaaagtgaa gggaggaaga agaaatgact cagacaacga ttggtgttgt
    41161 ggttgaaaaa agcagggata atctcgtttt tgtgacagaa attcaaacag gtagagcttt
    41221 tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc aatattttaa cgttaaatct
    41281 gactacgaag atgttcgttg atgcagtaga aaattatcca tttgtttaga caaacgatat
    41341 tttacaagga ggaaacggac atggcagaag aaaaagaacc actagataat agtcgtttgg
    41401 gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt aaataatcaa tttcaaaagc
    41461 tatttgatca ccaaaaattg acaaatgggc aaccaatgaa tgacaaacga aatggtcgtt
    41521 catggtttaa ccagcaagaa aaaatagaaa ataaaataca aagtttgaga gaagagatca
    41581 agcaacaaga aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggtt
    41641 ataacaagta cggtggcctg gacatgacga tcgaaaatat tccaagaatc aaagaagaaa
    41701 tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga aaataccaac
    41761 gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc
    41821 ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa aagaatccaa
    41881 ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa aacgggggat
    41941 ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct gttcgtgaat
    42001 tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat catttctatc
    42061 gagtagaatt caacgaaaat gatccagcaa aagggttgaa aagttatggt ggggagatcg
    42121 taacactaga attgatcgat caactcaaaa cactggataa ccaatacgcc gcagaagcag
    42181 gctattataa attctatttt gaagaagtga agaatggaga agtagtcgat cagatacgaa
    42241 tggatctagg ggatggcgtc aatagtaatc accccatcta tttagagtta gctagattgt
    42301 gcgaacaatc acctgaaaag gaacaagaac aaacagtcaa aaatgaacct atttcaaaag
    42361 tacaaaacga gattaaagag acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag
    42421 aagagattaa gcaagaagtc aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc
    42481 atactcattc acctgaagac gtcaaagaac ttttgaattt tatgtctcgt tttccagaac
    42541 gatcctttag aaatcaatta ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag
    42601 gtcgtgccgc gttgaaaaaa gaaggaatct atatcaaaaa aggagaacaa gggaacaaga
    42661 tctttgtcag aaaaacggtc aaaggatttt atgagaaagg acgtggcttt gttcgagaaa
    42721 cagaggctac accagaagat aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa
    42781 aaccttacta cacgattgaa aaagtgtttg atatcacaca aacacaattg aaacctgaag
    42841 actatccgaa aatcttcccg aaccgcgtct ttgattttca attggatcaa acagggcaga
    42901 cagaacttca agttgggatt caagcggtag ccaaaaatat tggaattcaa attcgagata
    42961 tgacagaaag tgaagtctat caaagagaat taggacaagc acgtggggcg tacgttcgta
    43021 acgaatttac tggcgaagaa gaaatcgtca tgaatactcg aaatactcct acgcagcatt
    43081 tagccacaag tattcacgaa ttagcccatg ctagaatgca caaattttca gaattagata
    43141 ctgcaacaaa agaactacag gcagaaatga cctcctatat tgtgtgcaag cattttggga
    43201 tggacacctc ggagaaagct attccttata ttgcgacatg gacgaagaat agtcagacgt
    43261 tggatgataa ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa
    43321 atgaattcat tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga
    43381 tccagccagc agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga
    43441 atattgtgcc cgttggctca ctttggatcg acaagaaaga cgggaaagtc ctggaagaag
    43501 acacgggccg aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc
    43561 aagtgatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag
    43621 aatttgcgct aggaaatcca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac
    43681 gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc
    43741 aagaaagact atctgaaaaa tggatagtct tttttctttt gggaggtttg aaaatgacag
    43801 agaaaaaaat gagtctcatt gatcgatgca agcaaatgga tattgtggat tttgcacgaa
    43861 acaatggttt agcggtggcc aataaaggac gagactatcg cttagaagac catgattcat
    43921 ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca
    43981 ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat
    44041 ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc
    44101 ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa
    44161 aaggaaggaa ttatctgaag gaagaacgaa aactgcccga ttggttgatt gattatgcag
    44221 aaaaagaagg attgattgcc gaactaaagc caaaacatga acggcaaaat ttcttagttc
    44281 gagacgatcg tttggatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga
    44341 cagtaggggc gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca
    44401 catataagca tatagataaa aattcgacag cgaatcatgg ctttaaccta aagattggtg
    44461 atcctaaaca gttaaaattt tttgagagta gtattgattt acttagctac gctgcactga
    44521 atcgtgatca gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga
    44581 tcagtcatta ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat
    44641 ccatcgagat ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat
    44701 taatgggcgc agttgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta
    44761 atgattggca agtgccaaga gaatacaaag tcatttatga agaagtcgca aaagaagaaa
    44821 aggtcacacc agaagctatt atggccatac acaagacaga aaacaatctg caattaacga
    44881 accaactggt ttccgctcac aaagtcaatg cgtcttttgg tcagcagctt tctgttaacg
    44941 attcaataga agtcattagt ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag
    45001 attgtgaacg ctcgaatggt acgtatgatt ttgatcgttt ttatcaagga aaaggggata
    45061 ttaattctca gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc
    45121 atgaacatga atttgtacct gaagtgaaaa aggattggaa cgatcaattg aagcatgaga
    45181 tccatcaaca ggaaattaga aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac
    45241 aagaaaggga gtgagatcgt cacgatgaaa actgtcattt tagcagaaaa accgtcacaa
    45301 gcgaaggcct atgccgatag cttttctaaa gccacccgaa aagacgggta ttttgaaatc
    45361 caagatcgat tgttttcggg tgaaactgtg atcacgtatg gttttggtca tttagtcgag
    45421 ttagattcgc ccgatatgta cgatgaaaat tggaaacaat ggtcattaga gcatttgcct
    45481 attttcccta cacattatca ctatcatgtt cctaaagata agaaaaagca attcaacgtg
    45541 gtgaaacagc aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa
    45601 ggggaactga tcgcttggac gatcattcaa caagcaggcg ctgatcaagg aaaaaccttt
    45661 aaaagacttt ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcaatta
    45721 cgagacgcag aagagaccta tcccaaattt gaagaagcac aagcacgcca gatcgctgat
    45781 tggttgatcg gcatgaacgg cagcccactg tatagtttac tcttgcagca aaagggcata
    45841 ccaggaagtt tttccttagg cagagtccaa accccaacgc tttacatgat ttatcagctg
    45901 caggaaaaga ttagaaactt ccaaaaagag ccttattttg aagggaaagc ccaagtaacc
    45961 gctcaaaatg gcatatttga tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa
    46021 gcatttgaag cctatttaaa agaaaaaggt gttcaagtag gaaagcagcc aggcacgatt
    46081 cttgaagtcg aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc
    46141 cttcagtcta aaatgaatca gctcatgaaa gctagtgcca aagatacttt ggaagccatg
    46201 caagggttgt atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattaca
    46261 gaaggagagc atgcgtatct gttggatcat ttagatgaat acaagcattt tttaaaggct
    46321 gaatcattcc ctactcctat tcatacaccg aatagtcggt atgtgaataa taagaaagtc
    46381 caggaacatt acgcgatcat tcctacaaaa acagttatga cagaagcaac gtatgaaaag
    46441 ctgacacctt tacaacaagc gatttatgat caagttttaa agacgacggt ggccatgttt
    46501 gcggaaaaat atacctatga agaaactacg attttgacac aagtccagca gcttcagcta
    46561 aaggcagtcg gaaaagtacc agtagatcta ggttggaaaa agctatttgg caaagagagc
    46621 gacagaaaag agaaggaaga agaaccgcta cttcctaaag taacaaaagg agaaacggtg
    46681 acggtagatc tacaaatctt tgaaaaagaa accaagccac cacaacctta cacggaaggt
    46741 acactaatca ccgccatgaa gacagctggt aaaaccgtgg atagtgaaga agcacaatcc
    46801 atattgaaag aagtggaggg aattggtaca gaagcgacac gagcaaatat tattgaaacc
    46861 ttgaagcaaa aggaatatat caaggtagag aaaaataaac ttgtggtgac gaataaagga
    46921 attttgcttt gtcaggctgt tgaaaaagaa ccgctgttaa caagtgccga aatgaccgcc
    46981 aaatgggaaa gctatcttac aaagattggt gagcaaaaag ggacacaagc tacttttcta
    47041 gcaaatatcc aaaaatttgt ttcccacttg ttggaagtcg tcccaggaca aatccaagcc
    47101 actgattttg gagcaacgtt acaagaggtg aaagtagcca gtgaaaagca agaagcggcg
    47161 cgtcatttag gatcttgtcc aaagtgccag gaaaaagaag tcatacttta tcagaaggtg
    47221 gcgacttgca cgtctgaagc ctgtgatttt aaattgtggg taacgattgc caaaaagaaa
    47281 ctcacagcga cacaactgaa agagatcatt cagaacggcc gaacaagtca accagtcaga
    47341 ggactaaaag gtcagaaagg ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca
    47401 acaggatttg agttttctga aaagaagaag accacctaca aaagaaaagt gagacgtaaa
    47461 acgagttaga attgacggag ggaaaaacga tgagaaatac aacgcataaa gaacaagaaa
    47521 tcgaaaatca aaaagccaaa atacgttcag agcaggagaa aatgcgtgcc agcaaacgaa
    47581 gactacagca attgaagaat caacaacgga ttcaaaaaga acttgaatta gatcgataaa
    47641 tcctcctgtt gttaatatta aactaacatg ttatagttat gttagtttaa tattaacatt
    47701 cgagagggaa agcgatgaaa gataaagtca agtgcatttt tgagaaatac aatggtaatc
    47761 tatccttgaa ggatgctaga aatgaagggt tatcacctat gacgttagat cgtttagccg
    47821 aaaaggacaa aatagaaaaa atagcatcag gtttttatac cttaaaagat agttttgtgg
    47881 atgaattata tctcgctcaa agtatctata ctagaggaat cttctctcac gagacagcgc
    47941 ttgatctttt tcaagtagga acatatatcc ctaaaaaaat taatatgatg tttcctaaag
    48001 gatacaatgt ttcgaaagag aatttagatc agtatgccat tcaaccgcat tacacgaata
    48061 aagaaaactt tgaactggga atcacagagg cagaaagctt ttacggcaat aaaattttcg
    48121 tctatgattt agagcgaacg ttatgtgata tgtggaatcc cagatataaa gcaagcgtag
    48181 aagtaaaaca agaagcatta aaagaatata tgacaagtac cagcagaaat acaaataagc
    48241 taagaagata catgaatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    48301 tttattgaga ggagtaaagg tgaatgacac cgacacaatt taaagcgaaa acaagaaatg
    48361 ttgcagctga gaaacattta aacccacaaa ttgttcaaag atattatatg atggaaaaat
    48421 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    48481 tgatcggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    48541 gaaattcgaa actaacaaaa gaaaaattag aaactgtttt ttctgaatta acagctgctc
    48601 ctacaaaaga tggcattcgg tttgaattaa agggcttaac agagacgagg gaagccgatt
    48661 attacccagg ttttcaagca aagtttattg cttttcttga aaatgcacgt gtacctttta
    48721 aattagatat tacttcaggt gattctatta ttccagcagc attgatatat gaacataaat
    48781 tgatgtttga agataaaaca attcatattc cagcttatcc tacggaacaa attttagctg
    48841 aaaaattaca tgccacgttt agtttcggaa tagataattc acgtatgaaa gatttctatg
    48901 atctgtatat gatccctaaa ctagaacgta tcgatgagat aaatttgtat aagagtgttc
    48961 aaggaacatt taaggaaaga aataatcaat cagtgtttaa gaatttgtat aatcaggaaa
    49021 tgcctcaatt gatgaagaat gagggattga gggagaaatg gaaaagatat cagcaagaaa
    49081 actattttgc caaagatatt tctttcgaat ctaccttaac ttctgttgat tcgctgatgc
    49141 aatcaattgt cgagcaagag gaagtagaac gaaaacaaaa ttttaagaga tcacaacaaa
    49201 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag gtcgattaac
    49261 caaagatccc gatttacgtt acaccgcaag tggcactgca gtgaccactt ttaccttagc
    49321 cgttaaccgt aattttacta atcaaagtgg caatcgagaa gctgacttta tcaattgtgt
    49381 gatttggcgc aaaccagctg aaacgatagc gacattagct aaaaaaggca gcttgatcgg
    49441 cgtcgtcgga cggattcaaa cgcggactta tgacaaccag caaggacagc gtgtctatgt
    49501 gactgaagtt gtggccgata atttccaact attagaaagt aaagcagcga cagaaagccg
    49561 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    49621 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    49681 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    49741 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    49801 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    49861 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    49921 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    49981 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    50041 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    50101 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    50161 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    50221 gatgatggaa aat
//
LOCUS       DADOOS010000006        45108 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.006, whole genome shotgun sequence.
ACCESSION   DADOOS010000006 DADOOS010000000
VERSION     DADOOS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.006"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            446..1549
                     /locus_tag="J8C37_000290"
     CDS             446..1549
                     /locus_tag="J8C37_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAZ5303756.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1833..2513
                     /locus_tag="J8C37_000291"
     CDS             1833..2513
                     /locus_tag="J8C37_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAZ5303757.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2603..3637
                     /locus_tag="J8C37_000292"
     CDS             2603..3637
                     /locus_tag="J8C37_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAZ5303758.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3651..3986
                     /locus_tag="J8C37_000293"
     CDS             3651..3986
                     /locus_tag="J8C37_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303759.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3970..6450
                     /locus_tag="J8C37_000294"
     CDS             3970..6450
                     /locus_tag="J8C37_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303760.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6506..8065
                     /locus_tag="J8C37_000295"
     CDS             6506..8065
                     /locus_tag="J8C37_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5303761.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            8062..9426
                     /locus_tag="J8C37_000296"
     CDS             8062..9426
                     /locus_tag="J8C37_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ5303762.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9597..10667
                     /locus_tag="J8C37_000297"
     CDS             9597..10667
                     /locus_tag="J8C37_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303763.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10682..11350
                     /locus_tag="J8C37_000298"
     CDS             10682..11350
                     /locus_tag="J8C37_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303764.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11370..11948
                     /locus_tag="J8C37_000299"
     CDS             11370..11948
                     /locus_tag="J8C37_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5303765.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12351..13262
                     /locus_tag="J8C37_000300"
     CDS             12351..13262
                     /locus_tag="J8C37_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ5303766.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13518..14435)
                     /locus_tag="J8C37_000301"
     CDS             complement(13518..14435)
                     /locus_tag="J8C37_000301"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAZ5303767.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14637..15074
                     /gene="zapA"
                     /locus_tag="J8C37_000302"
     CDS             14637..15074
                     /gene="zapA"
                     /locus_tag="J8C37_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAZ5303768.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15132..15679
                     /locus_tag="J8C37_000303"
                     /pseudo
     CDS             15132..15679
                     /locus_tag="J8C37_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            15794..18154
                     /locus_tag="J8C37_000304"
     CDS             15794..18154
                     /locus_tag="J8C37_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAZ5303769.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18241..18555
                     /gene="trxA"
                     /locus_tag="J8C37_000305"
     CDS             18241..18555
                     /gene="trxA"
                     /locus_tag="J8C37_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAZ5303770.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18737..20515
                     /gene="uvrC"
                     /locus_tag="J8C37_000306"
     CDS             18737..20515
                     /gene="uvrC"
                     /locus_tag="J8C37_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAZ5303771.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20660..21559
                     /locus_tag="J8C37_000307"
     CDS             20660..21559
                     /locus_tag="J8C37_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303772.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21552..22814
                     /locus_tag="J8C37_000308"
     CDS             21552..22814
                     /locus_tag="J8C37_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303773.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22977..24875
                     /locus_tag="J8C37_000309"
                     /pseudo
     CDS             22977..24875
                     /locus_tag="J8C37_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
     gene            complement(25174..25893)
                     /locus_tag="J8C37_000310"
     CDS             complement(25174..25893)
                     /locus_tag="J8C37_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAZ5303774.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26230..26562
                     /locus_tag="J8C37_000311"
     CDS             26230..26562
                     /locus_tag="J8C37_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303775.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26549..28552
                     /locus_tag="J8C37_000312"
     CDS             26549..28552
                     /locus_tag="J8C37_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAZ5303776.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28571..29041
                     /locus_tag="J8C37_000313"
     CDS             28571..29041
                     /locus_tag="J8C37_000313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAZ5303777.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            29062..29646
                     /locus_tag="J8C37_000314"
     CDS             29062..29646
                     /locus_tag="J8C37_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAZ5303778.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29661..30647
                     /locus_tag="J8C37_000315"
     CDS             29661..30647
                     /locus_tag="J8C37_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAZ5303779.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30637..30948
                     /locus_tag="J8C37_000316"
     CDS             30637..30948
                     /locus_tag="J8C37_000316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAZ5303780.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30970..32751
                     /locus_tag="J8C37_000317"
     CDS             30970..32751
                     /locus_tag="J8C37_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAZ5303781.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32744..34120
                     /locus_tag="J8C37_000318"
     CDS             32744..34120
                     /locus_tag="J8C37_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAZ5303782.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34127..34762
                     /locus_tag="J8C37_000319"
     CDS             34127..34762
                     /locus_tag="J8C37_000319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAZ5303783.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34792..36147
                     /locus_tag="J8C37_000320"
     CDS             34792..36147
                     /locus_tag="J8C37_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAZ5303784.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36339..37733)
                     /locus_tag="J8C37_000321"
     CDS             complement(36339..37733)
                     /locus_tag="J8C37_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAZ5303785.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37825..38796
                     /locus_tag="J8C37_000322"
     CDS             37825..38796
                     /locus_tag="J8C37_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAZ5303786.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38793..39575
                     /locus_tag="J8C37_000323"
     CDS             38793..39575
                     /locus_tag="J8C37_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAZ5303787.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39744..40115)
                     /locus_tag="J8C37_000324"
     CDS             complement(39744..40115)
                     /locus_tag="J8C37_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAZ5303788.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40249..40692
                     /locus_tag="J8C37_000325"
     CDS             40249..40692
                     /locus_tag="J8C37_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAZ5303789.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40735..41544)
                     /gene="yidA"
                     /locus_tag="J8C37_000326"
     CDS             complement(40735..41544)
                     /gene="yidA"
                     /locus_tag="J8C37_000326"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAZ5303790.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41563..42930)
                     /locus_tag="J8C37_000327"
     CDS             complement(41563..42930)
                     /locus_tag="J8C37_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ5303791.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42914..43744)
                     /locus_tag="J8C37_000328"
     CDS             complement(42914..43744)
                     /locus_tag="J8C37_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAZ5303792.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43907..44344
                     /locus_tag="J8C37_000329"
     CDS             43907..44344
                     /locus_tag="J8C37_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAZ5303793.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44420..45040
                     /locus_tag="J8C37_000330"
     CDS             44420..45040
                     /locus_tag="J8C37_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAZ5303794.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14775 a         7742 c         9594 g        12997 t
ORIGIN      
        1 tgaagtcaac gttctgacct gagtattaga gagacaaatg gcaaaatagt tcctcatatt
       61 ttgccatttt ttgagctaat accgagggtc agctgattga acaccgtttt atcgagaggt
      121 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
      181 tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt ttattcaaaa
      241 aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta tttttattac
      301 aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag attatttgcc
      361 tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt tcatcattca
      421 aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta caaatgaagg
      481 aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag tgattccagc
      541 tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc aagaaaatga
      601 acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag aattacccga
      661 tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg caccagttgc
      721 tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg tagcagattt
      781 tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga taagagaagc
      841 aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata atggaatcaa
      901 tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat tgacagcgga
      961 tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct tcccgctgcc
     1021 aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg ctgtatataa
     1081 atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc attctgattt
     1141 gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat tagaatctgg
     1201 tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg ggcctgctgc
     1261 gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa tcgtgtttga
     1321 cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg ccgatcttgt
     1381 tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag gagtccatca
     1441 agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg gaacacaaac
     1501 agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag aaataaatga
     1561 cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt tcaaggatgt
     1621 cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca tacttaataa
     1681 aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat acaaaaaggg
     1741 tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt ctctactata
     1801 cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt gggacattat
     1861 tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag ggtcaagcga
     1921 cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct cttaacccta
     1981 ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact ggccagacag
     2041 gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag attgaaagcg
     2101 gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc caagtatcag
     2161 atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca gtttcggttt
     2221 cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt ggaacatcca
     2281 ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact gtaactgggt
     2341 tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa ggtctgggaa
     2401 catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa gcagcagctg
     2461 gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca tagagtatga
     2521 aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta ccgcagttgt
     2581 tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt cgtttttttc
     2641 tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt cagcaggaga
     2701 ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg gttattttga
     2761 tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca attcttcaga
     2821 tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg gtggaacgat
     2881 cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg tccgtgacgt
     2941 tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa ctatacctat
     3001 agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta ttcatgtttc
     3061 acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca aaaatcgtat
     3121 tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac cggatctgaa
     3181 acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc ttcgtttcca
     3241 aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct tttatgaaaa
     3301 tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt cgaattttca
     3361 cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg cagaaatcat
     3421 agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga tcaaaaaaga
     3481 aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat ctttcccatg
     3541 gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc tgtttttgtg
     3601 ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg ttgaaaacat
     3661 cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa gagccagtcc
     3721 aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca gttccgcatc
     3781 caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt tataaagaat
     3841 ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt ctttatttga
     3901 tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga aaggaaaagg
     3961 aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt ttttacttcg
     4021 ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg agcaacgcct
     4081 cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa tggtggacgt
     4141 agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt gcctaaattg
     4201 actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac ggtccgtttt
     4261 cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat cactgcacaa
     4321 ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag tgatgtatta
     4381 aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat caaggttact
     4441 agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg gtcgtgggat
     4501 acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt caacgtggac
     4561 gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga aaacggagat
     4621 aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc ttctatgtct
     4681 aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca agatcgtatg
     4741 ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac tggcggaaac
     4801 ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg gaatagttac
     4861 atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac agaatacgtg
     4921 gatggaaata accagaacct tccaggctct accaatagtc ggacattgac gatggggctg
     4981 actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt caactatttt
     5041 acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag cacaagtgga
     5101 ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg attgctttct
     5161 cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt accaacaaac
     5221 ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca agtcacggcc
     5281 aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta ttcgaaagga
     5341 acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca atcaaataac
     5401 gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca atattatttt
     5461 gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc gtacaaatgg
     5521 cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa ttttccatct
     5581 gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg agatcccctg
     5641 tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac aaaatctggg
     5701 gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac ttatatcaat
     5761 ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca aggagtttct
     5821 gtcccagcag atctattgag caaaatagga gataccattc atcttactta caactatcaa
     5881 ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc agctgtactt
     5941 tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt ccaaacatct
     6001 gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc agatgatttt
     6061 acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc aaaaggcgat
     6121 ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca actgacaggg
     6181 acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac aaaattatac
     6241 ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt gatttatgag
     6301 agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga tggtttactc
     6361 ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg catggtcaca
     6421 tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga tctgtgataa
     6481 ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa aatcagaaat
     6541 gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac aacggcaaat
     6601 caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata acgtacttac
     6661 ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac aagctaactt
     6721 ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat taatcagaca
     6781 tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt ctacaaaata
     6841 tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac ttgtcatgga
     6901 acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg attctttaaa
     6961 tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc aaatttgtta
     7021 tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa aagaatacag
     7081 tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt ttactattac
     7141 agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc gaatgcgctg
     7201 tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc aaacgacaca
     7261 atttttattg gacctccata catttttgac ccgttacttc tttcaatatg cctttccttg
     7321 gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta cattagatac
     7381 acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg acgtagtagc
     7441 tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt atgtttttcc
     7501 agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc attttgccta
     7561 ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt taactgaagt
     7621 agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat ttgctattgt
     7681 tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc gctatgcatc
     7741 tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt atgatcttcc
     7801 ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat tgctgaatac
     7861 gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca ttttgactag
     7921 ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca ctagcaacga
     7981 atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt tagaaaaatt
     8041 gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca tgcaggcatc
     8101 acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact gatcgatgaa
     8161 gatcataaag atggactagg ctatgtttca aatggaatca acctttattt aaaaggaaag
     8221 atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag tggggcaaaa
     8281 ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct catcctcgca
     8341 tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc aacaggttcc
     8401 tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta taaaaatcta
     8461 gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt gatcttcggt
     8521 gctggttata ttggattgga attagcagat gctttgagaa ataaaggata cattattcat
     8581 ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat gatcaacagt
     8641 tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt cttgatcgat
     8701 tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc aataaaggca
     8761 gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa agaaaaagtg
     8821 gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag tgatccggac
     8881 atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg gcatctcttt
     8941 ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac tttgagtggt
     9001 cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat agactacttt
     9061 ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa taccggttcg
     9121 tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac agtaaatgca
     9181 aagctgatct accatccgga tacattagaa ataattggcg gacagctgat aagccaagaa
     9241 ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac tacgattcca
     9301 cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt tcactacctc
     9361 aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt agataaaaga
     9421 ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta atcaatcgag
     9481 aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata ttcgccaaaa
     9541 atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg agttaaatgt
     9601 ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt gcaggggtga
     9661 tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc ttagctcaag
     9721 ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg aaagatgcaa
     9781 attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc gaaggtggga
     9841 aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt acagaagaag
     9901 gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg cccaatcttg
     9961 ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt ttgaaagaag
    10021 aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag acgtttaaac
    10081 taactggctt tacggatcat gccaaattca atgtctcacc agttctctat acaaccatca
    10141 atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca cggattaacg
    10201 caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag atcaaaattt
    10261 ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt ggatttatga
    10321 tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat gtactgacga
    10381 tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga ggatttattg
    10441 cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt cttggtctat
    10501 taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa agcaattggc
    10561 tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc gcactatttt
    10621 ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa ggaggaagag
    10681 catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg accaagtagt
    10741 taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg caatcatcgg
    10801 tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc agacgcctag
    10861 cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa gtgaacgcgt
    10921 taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg ttccttttct
    10981 gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc gtcaaaaaga
    11041 agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt atccagaaga
    11101 actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca atgatccgtc
    11161 cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac gagaagtagt
    11221 cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg ttactcacga
    11281 tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg tattgaaaga
    11341 aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa gatacacagc
    11401 aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc ggcacttcaa
    11461 caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat tatttcggtg
    11521 ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt ggaaaagctt
    11581 tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca aaaatggcgc
    11641 agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat cttgaaagca
    11701 atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat tatcgtgaac
    11761 cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt tcaaaagaaa
    11821 tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg aaaagacctg
    11881 tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt gtattaggag
    11941 aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag atatgacgaa
    12001 gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa tttgttattc
    12061 tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga agtgttttaa
    12121 tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga
    12181 atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt
    12241 agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc gtacttaagc
    12301 tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa
    12361 taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt
    12421 ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga
    12481 caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc
    12541 gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc
    12601 tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata
    12661 ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg
    12721 aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg
    12781 cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag
    12841 taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat
    12901 tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca
    12961 gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt
    13021 actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc
    13081 tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt
    13141 ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag
    13201 acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt
    13261 aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat
    13321 tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc tgccgtttaa
    13381 ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac
    13441 ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac tgtgacatga
    13501 cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt attggcaaaa
    13561 tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag ttttgccgct
    13621 acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc agctgatgct
    13681 ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc aacagctaaa
    13741 tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt tttttctaaa
    13801 cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat cgcttctggt
    13861 ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa agcgactttc
    13921 atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga taagacaagc
    13981 agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg gctatctttt
    14041 aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc acgtttcaca
    14101 taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc atcactacct
    14161 aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc tggcttggct
    14221 tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt accttgaaac
    14281 atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta
    14341 gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat ttgactaagt
    14401 tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt tttatacata
    14461 aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca tagcattttt
    14521 ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga
    14581 accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg attcgtatgg
    14641 cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact attatcggac
    14701 gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag ttagcagaat
    14761 tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg gctgtcaatg
    14821 cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa gaaacagcag
    14881 aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag
    14941 caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat cataccatta
    15001 aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa attaaacaaa
    15061 aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt tagaagagaa
    15121 aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg tttttttctg
    15181 gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta ttgtcattta
    15241 ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac attccatacc
    15301 cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct tttcacctag
    15361 atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc tggctgctta
    15421 cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg agacaggtag
    15481 actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata tttttgattt
    15541 tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg agcatagttt
    15601 cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg agctttgggt
    15661 aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt tgtacagagt
    15721 agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc atagtgaaag
    15781 ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa aagtcaaaca
    15841 gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag aattggtacc
    15901 agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg gattgaaagt
    15961 gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc cccatatgaa
    16021 acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt ccagagtatt
    16081 gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg aaatcgaatt
    16141 tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga gccgccgttt
    16201 aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg agttaaagag
    16261 cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag atgggatcgt
    16321 ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa atgaccgcta
    16381 cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag
    16441 tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga ataatcgttt
    16501 gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg aactttctgc
    16561 tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg gtaaaatgga
    16621 tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag
    16681 tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt
    16741 agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta caggacctaa
    16801 cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga tggggcaagc
    16861 aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg aagtatttgc
    16921 ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt ctcatatgac
    16981 gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct
    17041 cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct tagatgatct
    17101 tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga aagtttatgg
    17161 atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata cactaagtcc
    17221 aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg
    17281 attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg atgagagcca
    17341 agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag agaccgaata
    17401 tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt tgaaagaagc
    17461 ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa
    17521 cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat
    17581 gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg caaagaccca
    17641 attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga agaaggcgaa
    17701 agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg gccaaagagg
    17761 gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa
    17821 tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac aacgagttat
    17881 ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc ttcgaggaaa
    17941 acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca ttctagctgg
    18001 ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca agggaattac
    18061 tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg
    18121 cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt
    18181 cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga ggtctttgat
    18241 atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg acttgtattg
    18301 attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag
    18361 ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt ggatgaaaat
    18421 ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt gaaaaaagat
    18481 ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg tcaaatgatc
    18541 gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg aatccagttc
    18601 cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt ttgagtataa
    18661 ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt taattgaata
    18721 tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac ttcctgatca
    18781 gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg gcaaagccaa
    18841 aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga aaacggaacg
    18901 gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata tcgaagcatt
    18961 gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta tgttaaagga
    19021 tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt tggtgattac
    19081 tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg tcggtgcggc
    19141 gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca aacctagtca
    19201 gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt attatttcga
    19261 cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg
    19321 acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg aaaacatgga
    19381 gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa tcatgactcg
    19441 ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg cgattgacaa
    19501 aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg aacgagatgt
    19561 ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc tcggtcagtt
    19621 ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata cgatcgacaa
    19681 gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa
    19741 aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac ggtttgatct
    19801 gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa
    19861 tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg ggacgaatcc
    19921 tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat atcggaaata
    19981 taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag tcatctatcg
    20041 cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct tgatcgatgg
    20101 aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag gaatcgatat
    20161 tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc tattcggtat
    20221 agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac tccagcgaat
    20281 ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca gtaaaaacag
    20341 ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa aaatgctgct
    20401 taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat tacaagaaat
    20461 cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg aatgaggaaa
    20521 gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa aagagcgttt
    20581 acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat atgtgatttc
    20641 ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt ttgggaacta
    20701 cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg ggctaattgg
    20761 ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt tgacacaaga
    20821 ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg atgtcatcgg
    20881 ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc aactgcttta
    20941 ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg atttttggat
    21001 ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa
    21061 tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga tcattttgga
    21121 tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg gaatattgga
    21181 attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata atgtagaaaa
    21241 aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca
    21301 tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt taagccaaca
    21361 ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca atacgttaga
    21421 gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga ctcagttcgg
    21481 ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca aattgaaagc
    21541 aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta aaaaatgtta
    21601 agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt atcatttatg
    21661 tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta ttggctgata
    21721 atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag ttcaaagcag
    21781 ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat gcgtatttag
    21841 tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat tcattaggac
    21901 aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg cttcgagcaa
    21961 gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca agtttgactc
    22021 gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat aactcgacag
    22081 tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt ttgacgtatg
    22141 cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc
    22201 tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt ttacttgttg
    22261 cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag
    22321 atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat
    22381 tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag
    22441 ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga
    22501 cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca gatccaaaca
    22561 atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt atgccggttc
    22621 gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag
    22681 ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgtgt
    22741 atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg agaaatgaac
    22801 gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat tgttcccagc
    22861 ttcttttttg tatagaccta tttctatttt tttattatat tggatatata tattcattga
    22921 aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc gttttaatgg
    22981 accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc atcacggaaa
    23041 tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagcgata
    23101 tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag
    23161 aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc caattcacta
    23221 tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa caagataaat
    23281 cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa tacactcgat
    23341 caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata
    23401 tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa caacttgtct
    23461 tgatgaagga agaagaaaaa ttttaaacaa aacttgcgca gacgatccaa aaatctgtca
    23521 tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca gccaaagtaa
    23581 tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat gatattttgt
    23641 ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg attgccgcac
    23701 gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt ccattatttt
    23761 cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac aggaaagatc
    23821 tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct atcctccaat
    23881 tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac gatcgtaaat
    23941 tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt
    24001 tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc ttggaagagg
    24061 aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga atgcaaaaac
    24121 acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtactgt
    24181 tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa ttagaccaat
    24241 cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag
    24301 atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg agcgggccat
    24361 tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt ctcacagagc
    24421 ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa gagtggttca
    24481 gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac
    24541 acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga aaagcatttg
    24601 ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct ggctactcat
    24661 tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg cctattgaac
    24721 aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc
    24781 cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag attactcaac
    24841 tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca acattgctca
    24901 atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa
    24961 aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc aggtcagggc
    25021 gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc
    25081 acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagttg
    25141 aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa
    25201 taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag
    25261 gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc
    25321 gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa
    25381 gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt
    25441 tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga
    25501 aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac
    25561 tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc
    25621 tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact
    25681 aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga
    25741 atctggagac atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac
    25801 aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag
    25861 ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt
    25921 gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct
    25981 tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat
    26041 attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca
    26101 aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa
    26161 gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat
    26221 cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa
    26281 aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag
    26341 caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa
    26401 aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca
    26461 gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt
    26521 gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct
    26581 catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt
    26641 tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc
    26701 agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga
    26761 catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca
    26821 tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt
    26881 catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc taactgaaaa
    26941 acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc
    27001 tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa
    27061 ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga
    27121 ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat
    27181 agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc
    27241 taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa
    27301 gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga
    27361 agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta
    27421 tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt
    27481 aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga
    27541 gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt
    27601 gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac
    27661 accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat
    27721 gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt
    27781 cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc
    27841 atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct
    27901 atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca
    27961 gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag
    28021 tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat
    28081 tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat
    28141 tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc
    28201 tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta
    28261 tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct
    28321 agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt
    28381 acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca
    28441 gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa
    28501 aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga
    28561 tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt
    28621 agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac
    28681 aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat
    28741 tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt
    28801 tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct
    28861 gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg
    28921 tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat
    28981 ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta
    29041 agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa
    29101 gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac
    29161 tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc
    29221 gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa
    29281 gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa
    29341 atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta
    29401 cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag
    29461 acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact
    29521 gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc
    29581 agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc
    29641 gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag
    29701 agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag
    29761 cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat
    29821 atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat
    29881 cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga
    29941 aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg
    30001 gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc
    30061 ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc
    30121 ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg
    30181 ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata
    30241 tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac
    30301 tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg
    30361 aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc
    30421 gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat
    30481 tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg
    30541 caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg
    30601 aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt
    30661 ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca
    30721 accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt
    30781 tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa
    30841 atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa
    30901 gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag
    30961 atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag
    31021 aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga
    31081 gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga
    31141 attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca
    31201 gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc
    31261 acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa
    31321 tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg
    31381 atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga
    31441 acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa
    31501 accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg
    31561 ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat
    31621 acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg
    31681 aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg
    31741 ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt
    31801 gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac
    31861 atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc
    31921 cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca
    31981 cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat
    32041 cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca
    32101 gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt
    32161 tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc
    32221 agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat
    32281 tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc
    32341 accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta
    32401 gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt
    32461 gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa
    32521 caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg
    32581 ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga
    32641 cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc
    32701 aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg
    32761 tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata
    32821 tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga
    32881 agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg
    32941 tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat
    33001 cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga
    33061 aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga
    33121 gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa
    33181 attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg
    33241 acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat
    33301 cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg
    33361 ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg
    33421 tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat
    33481 catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga
    33541 agttccagga agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga
    33601 acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat
    33661 gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca
    33721 gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc
    33781 gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc
    33841 agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc
    33901 cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga
    33961 gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac
    34021 acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa
    34081 agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa
    34141 tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag
    34201 agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat
    34261 tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga
    34321 ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc
    34381 ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt
    34441 gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa
    34501 ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt
    34561 ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg
    34621 tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta
    34681 tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa
    34741 aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa
    34801 caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt
    34861 ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat
    34921 gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg
    34981 acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg
    35041 attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag
    35101 aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta
    35161 tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat ccaattgatt
    35221 ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg
    35281 tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac
    35341 agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt
    35401 tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag
    35461 atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc
    35521 gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa
    35581 cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt
    35641 gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc
    35701 ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc
    35761 cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga
    35821 caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc
    35881 gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt
    35941 gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac
    36001 ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta
    36061 ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat
    36121 ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt
    36181 aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg
    36241 tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt tttattgaaa
    36301 ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata tttttttatt
    36361 ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa
    36421 attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg
    36481 ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg atcaactgga
    36541 agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa
    36601 agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt tgcttcattt
    36661 gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta
    36721 aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata agcaactcgc
    36781 aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataaa
    36841 ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca
    36901 tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg
    36961 tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc
    37021 ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca
    37081 attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat
    37141 cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca
    37201 ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt
    37261 aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc
    37321 aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta
    37381 cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta
    37441 gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta
    37501 tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa
    37561 tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc
    37621 tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt
    37681 tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctcc
    37741 actccttaaa attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa
    37801 tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct
    37861 tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt
    37921 aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg caaaaatatg
    37981 tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa agtcattacc
    38041 gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt
    38101 gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga
    38161 acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt
    38221 gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt
    38281 cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct
    38341 aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca
    38401 gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa
    38461 gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga
    38521 gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctgcc
    38581 ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt
    38641 attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg
    38701 aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat
    38761 atttataaca aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg
    38821 catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc
    38881 tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta
    38941 cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc
    39001 tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg
    39061 atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt
    39121 attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg tctgaatatg
    39181 aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat
    39241 tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg
    39301 aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa
    39361 ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc
    39421 ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat
    39481 caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag
    39541 cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa
    39601 attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac gcaaactttt
    39661 tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt ttattcaatc
    39721 aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata accatcaggg
    39781 tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc agtaatctca
    39841 agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc tatagcgata
    39901 tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat
    39961 tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca
    40021 cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga
    40081 tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta
    40141 ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt
    40201 ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca
    40261 atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc
    40321 atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct
    40381 ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa
    40441 gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac
    40501 tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg
    40561 aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa
    40621 gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa
    40681 aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag
    40741 tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt
    40801 tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc
    40861 aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc
    40921 aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc
    40981 gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg
    41041 cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat
    41101 gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc
    41161 gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga
    41221 atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt
    41281 cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata
    41341 agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc
    41401 tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat
    41461 cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc
    41521 gatagcgact agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa
    41581 tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg
    41641 atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc
    41701 ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc
    41761 atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata
    41821 aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc
    41881 tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga
    41941 tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga
    42001 ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc
    42061 taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct
    42121 ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat
    42181 accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata
    42241 atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat
    42301 cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc
    42361 ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg
    42421 tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac
    42481 tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc
    42541 ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc
    42601 atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc
    42661 tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata
    42721 aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt
    42781 gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat
    42841 tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg
    42901 cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt
    42961 ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg
    43021 tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt
    43081 tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag
    43141 gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc
    43201 gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac atcataacag
    43261 aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta
    43321 aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg
    43381 gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt
    43441 ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc
    43501 ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt gtttggatcc
    43561 cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt
    43621 gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta
    43681 atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt
    43741 tcattcaacc ggctcctttg tataaattca tattttctac aatattatac tctaaaatca
    43801 aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa
    43861 agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca
    43921 aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga
    43981 tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta
    44041 taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga
    44101 tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa
    44161 agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac
    44221 gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc gaatcgatta
    44281 tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc
    44341 gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact
    44401 gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt
    44461 atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc
    44521 tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc
    44581 actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa
    44641 tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg
    44701 aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt
    44761 tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct
    44821 gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc
    44881 tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga
    44941 cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga
    45001 ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag
    45061 gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacga
//
LOCUS       DADOOS010000007        43441 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.007, whole genome shotgun sequence.
ACCESSION   DADOOS010000007 DADOOS010000000
VERSION     DADOOS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.007"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(105..629)
                     /locus_tag="J8C37_000331"
     CDS             complement(105..629)
                     /locus_tag="J8C37_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5303795.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="J8C37_000332"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="J8C37_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAZ5303796.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="J8C37_000333"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="J8C37_000333"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAZ5303797.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="J8C37_000334"
     CDS             complement(2351..3031)
                     /locus_tag="J8C37_000334"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAZ5303798.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="J8C37_000335"
     CDS             3157..4026
                     /locus_tag="J8C37_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5303799.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="J8C37_000336"
     CDS             4052..4567
                     /locus_tag="J8C37_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303800.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="J8C37_000337"
     CDS             complement(4614..5177)
                     /locus_tag="J8C37_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5303801.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="J8C37_000338"
     CDS             complement(5232..5411)
                     /locus_tag="J8C37_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303802.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="J8C37_000339"
     CDS             complement(5488..5997)
                     /locus_tag="J8C37_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303803.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="J8C37_000340"
     CDS             complement(6093..6473)
                     /locus_tag="J8C37_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAZ5303804.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="J8C37_000341"
     CDS             6803..7552
                     /locus_tag="J8C37_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAZ5303805.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="J8C37_000342"
     CDS             complement(7736..9013)
                     /locus_tag="J8C37_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303806.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="J8C37_000343"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="J8C37_000343"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAZ5303807.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="J8C37_000344"
     CDS             complement(9935..10879)
                     /locus_tag="J8C37_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAZ5303808.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="J8C37_000345"
     CDS             complement(10881..11567)
                     /locus_tag="J8C37_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAZ5303809.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="J8C37_000346"
     CDS             12041..12361
                     /locus_tag="J8C37_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAZ5303810.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="J8C37_000347"
     CDS             12515..13420
                     /locus_tag="J8C37_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5303811.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="J8C37_000348"
     CDS             13417..14190
                     /locus_tag="J8C37_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ5303812.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="J8C37_000349"
     CDS             complement(14371..15795)
                     /locus_tag="J8C37_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5303813.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="J8C37_000350"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="J8C37_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAZ5303814.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="J8C37_000351"
     CDS             complement(16517..18499)
                     /locus_tag="J8C37_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5303815.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="J8C37_000352"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="J8C37_000352"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAZ5303816.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="J8C37_000353"
     CDS             complement(19412..20473)
                     /locus_tag="J8C37_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAZ5303817.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="J8C37_000354"
     CDS             complement(20684..21211)
                     /locus_tag="J8C37_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ5303818.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="J8C37_000355"
     CDS             complement(21278..22780)
                     /locus_tag="J8C37_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAZ5303819.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="J8C37_000356"
     CDS             complement(23038..24159)
                     /locus_tag="J8C37_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ5303820.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="J8C37_000357"
     CDS             24285..25226
                     /locus_tag="J8C37_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAZ5303821.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="J8C37_000358"
     CDS             complement(25376..26272)
                     /locus_tag="J8C37_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAZ5303822.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="J8C37_000359"
     CDS             26541..27881
                     /locus_tag="J8C37_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5303823.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="J8C37_000360"
     CDS             28099..28542
                     /locus_tag="J8C37_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAZ5303824.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMTVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="J8C37_000361"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="J8C37_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAZ5303825.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="J8C37_000362"
     CDS             complement(30867..32090)
                     /locus_tag="J8C37_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAZ5303826.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="J8C37_000363"
     CDS             complement(32121..32960)
                     /locus_tag="J8C37_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAZ5303827.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="J8C37_000364"
     CDS             complement(32977..33657)
                     /locus_tag="J8C37_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303828.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="J8C37_000365"
     CDS             complement(33659..34732)
                     /locus_tag="J8C37_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5303829.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="J8C37_000366"
     CDS             complement(35379..36050)
                     /locus_tag="J8C37_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ5303830.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="J8C37_000367"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="J8C37_000367"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAZ5303831.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="J8C37_000368"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="J8C37_000368"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAZ5303832.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="J8C37_000369"
     CDS             complement(39933..40799)
                     /locus_tag="J8C37_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5303833.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="J8C37_000370"
     CDS             complement(40959..43052)
                     /locus_tag="J8C37_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAZ5303834.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12545 a         9099 c         7664 g        14133 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatgacagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatatt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 g
//
LOCUS       DADOOS010000008        40616 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.008, whole genome shotgun sequence.
ACCESSION   DADOOS010000008 DADOOS010000000
VERSION     DADOOS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 40616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.008"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="J8C37_000371"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="J8C37_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAZ5303835.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="J8C37_000372"
     CDS             join(2859..2921,2923..3960)
                     /gene="prfB"
                     /locus_tag="J8C37_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAZ5303836.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="J8C37_000373"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="J8C37_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAZ5303837.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="J8C37_000374"
     CDS             4735..5628
                     /locus_tag="J8C37_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303838.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="J8C37_000375"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="J8C37_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAZ5303839.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="J8C37_000376"
     CDS             7565..8422
                     /locus_tag="J8C37_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAZ5303840.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="J8C37_000377"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="J8C37_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAZ5303841.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="J8C37_000378"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="J8C37_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAZ5303842.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="J8C37_000379"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="J8C37_000379"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303843.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="J8C37_000380"
     CDS             11178..11936
                     /locus_tag="J8C37_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303844.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="J8C37_000381"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="J8C37_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAZ5303845.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="J8C37_000382"
     CDS             12864..13895
                     /locus_tag="J8C37_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAZ5303846.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="J8C37_000383"
     CDS             13892..14659
                     /locus_tag="J8C37_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAZ5303847.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="J8C37_000384"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="J8C37_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAZ5303848.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16627..16944
                     /locus_tag="J8C37_000385"
     CDS             16627..16944
                     /locus_tag="J8C37_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAZ5303849.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            16944..17294
                     /locus_tag="J8C37_000386"
     CDS             16944..17294
                     /locus_tag="J8C37_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAZ5303850.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="J8C37_000387"
     CDS             17461..18399
                     /locus_tag="J8C37_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAZ5303851.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="J8C37_000388"
     CDS             18414..19244
                     /locus_tag="J8C37_000388"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAZ5303852.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="J8C37_000389"
     CDS             19288..20313
                     /locus_tag="J8C37_000389"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAZ5303853.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="J8C37_000390"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="J8C37_000390"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAZ5303854.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="J8C37_000391"
     CDS             21391..21831
                     /locus_tag="J8C37_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAZ5303855.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="J8C37_000392"
     CDS             21834..22436
                     /locus_tag="J8C37_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAZ5303856.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="J8C37_000393"
     CDS             complement(22495..23601)
                     /locus_tag="J8C37_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAZ5303857.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="J8C37_000394"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="J8C37_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAZ5303858.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="J8C37_000395"
     CDS             complement(25149..27494)
                     /locus_tag="J8C37_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAZ5303859.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="J8C37_000396"
     CDS             27773..28675
                     /locus_tag="J8C37_000396"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAZ5303860.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="J8C37_000397"
     CDS             28888..30663
                     /locus_tag="J8C37_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAZ5303861.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="J8C37_000398"
     CDS             complement(30729..32315)
                     /locus_tag="J8C37_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAZ5303862.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="J8C37_000399"
     CDS             32566..32790
                     /locus_tag="J8C37_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAZ5303863.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="J8C37_000400"
     CDS             32932..34683
                     /locus_tag="J8C37_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5303864.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="J8C37_000401"
     CDS             34683..36467
                     /locus_tag="J8C37_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303865.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="J8C37_000402"
     CDS             36793..37644
                     /locus_tag="J8C37_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5303866.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="J8C37_000403"
     CDS             37698..39449
                     /locus_tag="J8C37_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5303867.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="J8C37_000404"
     CDS             39453..39623
                     /locus_tag="J8C37_000404"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303868.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="J8C37_000405"
     CDS             complement(39663..40565)
                     /locus_tag="J8C37_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ5303869.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        13063 a         7176 c         8637 g        11740 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaat
//
LOCUS       DADOOS010000009        38668 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.009, whole genome shotgun sequence.
ACCESSION   DADOOS010000009 DADOOS010000000
VERSION     DADOOS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.009"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(369..995)
                     /locus_tag="J8C37_000406"
     CDS             complement(369..995)
                     /locus_tag="J8C37_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303870.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="J8C37_000407"
     CDS             complement(1290..1625)
                     /locus_tag="J8C37_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAZ5303871.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="J8C37_000408"
     CDS             complement(1612..1896)
                     /locus_tag="J8C37_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAZ5303872.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="J8C37_000409"
     CDS             complement(1952..3475)
                     /locus_tag="J8C37_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAZ5303873.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="J8C37_000410"
     CDS             complement(3468..4643)
                     /locus_tag="J8C37_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAZ5303874.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="J8C37_000411"
     CDS             complement(4647..4832)
                     /locus_tag="J8C37_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303875.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="J8C37_000412"
     CDS             complement(4798..6492)
                     /locus_tag="J8C37_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAZ5303876.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="J8C37_000413"
     CDS             complement(6489..6962)
                     /locus_tag="J8C37_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAZ5303877.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="J8C37_000414"
     CDS             complement(7031..7186)
                     /locus_tag="J8C37_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAZ5303878.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="J8C37_000415"
     CDS             complement(7320..7700)
                     /locus_tag="J8C37_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAZ5303879.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="J8C37_000416"
     CDS             complement(7704..7919)
                     /locus_tag="J8C37_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303880.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="J8C37_000417"
     CDS             complement(7922..8329)
                     /locus_tag="J8C37_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAZ5303881.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="J8C37_000418"
     CDS             complement(8595..10070)
                     /locus_tag="J8C37_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAZ5303882.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="J8C37_000419"
     CDS             complement(10060..10908)
                     /locus_tag="J8C37_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAZ5303883.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="J8C37_000420"
     CDS             complement(10945..11307)
                     /locus_tag="J8C37_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303884.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="J8C37_000421"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="J8C37_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="J8C37_000422"
     CDS             complement(12228..12539)
                     /locus_tag="J8C37_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303885.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="J8C37_000423"
     CDS             complement(12583..12876)
                     /locus_tag="J8C37_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303886.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="J8C37_000424"
     CDS             13070..13717
                     /locus_tag="J8C37_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5303887.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="J8C37_000425"
     CDS             13778..14923
                     /locus_tag="J8C37_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5303888.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="J8C37_000426"
     CDS             complement(15004..15309)
                     /locus_tag="J8C37_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAZ5303889.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="J8C37_000427"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="J8C37_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAZ5303890.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="J8C37_000428"
     CDS             complement(15762..16028)
                     /locus_tag="J8C37_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAZ5303891.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="J8C37_000429"
     CDS             complement(16283..17008)
                     /locus_tag="J8C37_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAZ5303892.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17369..17439)
                     /locus_tag="J8C37_000430"
     tRNA            complement(17369..17439)
                     /locus_tag="J8C37_000430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17405..17407),aa:Gly,seq:ccc)
     gene            17556..18308
                     /locus_tag="J8C37_000431"
     CDS             17556..18308
                     /locus_tag="J8C37_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAZ5303893.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18324..18803
                     /locus_tag="J8C37_000432"
     CDS             18324..18803
                     /locus_tag="J8C37_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAZ5303894.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19054..20634)
                     /locus_tag="J8C37_000433"
     CDS             complement(19054..20634)
                     /locus_tag="J8C37_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAZ5303895.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20627..21370)
                     /locus_tag="J8C37_000434"
     CDS             complement(20627..21370)
                     /locus_tag="J8C37_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303896.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21546..22322)
                     /locus_tag="J8C37_000435"
     CDS             complement(21546..22322)
                     /locus_tag="J8C37_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5303897.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22570..23406)
                     /locus_tag="J8C37_000436"
     CDS             complement(22570..23406)
                     /locus_tag="J8C37_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAZ5303898.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23417..24346)
                     /locus_tag="J8C37_000437"
     CDS             complement(23417..24346)
                     /locus_tag="J8C37_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAZ5303899.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24538..24891
                     /locus_tag="J8C37_000438"
     CDS             24538..24891
                     /locus_tag="J8C37_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ5303900.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24965..25621)
                     /locus_tag="J8C37_000439"
     CDS             complement(24965..25621)
                     /locus_tag="J8C37_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAZ5303901.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25847..27781
                     /locus_tag="J8C37_000440"
     CDS             25847..27781
                     /locus_tag="J8C37_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ5303902.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27867..29090)
                     /locus_tag="J8C37_000441"
     CDS             complement(27867..29090)
                     /locus_tag="J8C37_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAZ5303903.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29084..30982)
                     /locus_tag="J8C37_000442"
     CDS             complement(29084..30982)
                     /locus_tag="J8C37_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAZ5303904.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30976..32094)
                     /locus_tag="J8C37_000443"
     CDS             complement(30976..32094)
                     /locus_tag="J8C37_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAZ5303905.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32177..33010)
                     /locus_tag="J8C37_000444"
     CDS             complement(32177..33010)
                     /locus_tag="J8C37_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5303906.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33003..33920)
                     /locus_tag="J8C37_000445"
     CDS             complement(33003..33920)
                     /locus_tag="J8C37_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5303907.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33943..34122)
                     /locus_tag="J8C37_000446"
     CDS             complement(33943..34122)
                     /locus_tag="J8C37_000446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303908.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(34147..35271)
                     /locus_tag="J8C37_000447"
     CDS             complement(34147..35271)
                     /locus_tag="J8C37_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ5303909.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35454..36362
                     /locus_tag="J8C37_000448"
     CDS             35454..36362
                     /locus_tag="J8C37_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5303910.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36503..38116
                     /locus_tag="J8C37_000449"
     CDS             36503..38116
                     /locus_tag="J8C37_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAZ5303911.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11702 a         7709 c         6344 g        12913 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    17161 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    17221 tacactgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    17281 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    17341 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    17401 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17461 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17521 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17581 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17641 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17701 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17761 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17821 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17881 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17941 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    18001 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    18061 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    18121 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    18181 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    18241 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    18301 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    18361 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18421 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18481 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18541 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18601 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18661 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18721 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18781 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18841 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18901 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18961 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    19021 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    19081 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    19141 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    19201 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    19261 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    19321 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    19381 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19441 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19501 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19561 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19621 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19681 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19741 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19801 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19861 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19921 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19981 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    20041 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    20101 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    20161 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    20221 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    20281 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    20341 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    20401 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20461 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20521 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20581 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20641 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20701 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20761 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20821 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20881 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20941 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    21001 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    21061 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    21121 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    21181 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    21241 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    21301 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    21361 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21421 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21481 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21541 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21601 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21661 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21721 tcatattcga gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct
    21781 ttcaatacta ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca
    21841 tcgtaatgga tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta
    21901 gcatgttgaa caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta
    21961 tttttttcat aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata
    22021 acagttattg ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt
    22081 tctctaagca tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg
    22141 aaaaaaatat ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg
    22201 ttcaatccct gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa
    22261 atttctacta gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc
    22321 ataaaaagta cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca
    22381 tacatgttag gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga
    22441 tatcaaatac tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg
    22501 caaaaaaagc cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat
    22561 tttgttcttt taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc
    22621 gtttgtaaca actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat
    22681 ggttccttct attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt
    22741 caacaaacca aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa
    22801 gatatctgta ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt
    22861 gccaatgaat tcgctagggt aattataaat tagctccata acttctaaat agttttcatc
    22921 tgtgatcttg atagtttgct tatccccata cttttctttc aactcattca tttggtctaa
    22981 gtagtctgat ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc
    23041 cggatcccct actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt
    23101 agtgtcgaga ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac
    23161 ataagcgatt ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc
    23221 cttttcttct gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc
    23281 agataaaatc attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag
    23341 tttgccagaa acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg
    23401 cagcatattt tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc
    23461 cgatcagcga taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga
    23521 tatcgaccat tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta
    23581 acaaagaaga tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata
    23641 acagcatgat gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg
    23701 gtaaataagt ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat
    23761 caaaaaattc gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt
    23821 ttggttcatt cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta
    23881 ctttagacgt gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac
    23941 caatcacaag ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat
    24001 tgccaaatgc gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca
    24061 taaaagcaaa cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta
    24121 cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga
    24181 agatacgatt ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg
    24241 cgcctgagat aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga
    24301 aaatcgtact catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt
    24361 ttatttctag ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc
    24421 tgtaaatttt tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta
    24481 ttcccattaa attaaggtct catacatatc acttctatca taaaagggag aataaaaatg
    24541 aaacaaacca ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc
    24601 atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa
    24661 cttctcacac attatgatat tccggcactt catttggatg catttgaaac accagaagta
    24721 gctagtaaat ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa
    24781 gtctttcggc aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg
    24841 acagcagaag atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc
    24901 aaacagaaaa aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt
    24961 atttttattc tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca
    25021 aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca
    25081 atcgagcagc ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta
    25141 cttcttgtgc tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga
    25201 tttgttctga catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga
    25261 tttcctcatt cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt
    25321 attttagtaa agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat
    25381 cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac
    25441 gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa
    25501 cggcttcact taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta
    25561 aatagcgata atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca
    25621 cgaaatgacc tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt
    25681 ataggaaaaa atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat
    25741 ttatcatttc tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa
    25801 tagtttttta tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca
    25861 agccaatcag attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga
    25921 aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt
    25981 attaaagatc attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac
    26041 agcgactctc ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa
    26101 cgagatgctg aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga
    26161 gattttcatt agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata
    26221 cgaccggctg caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg
    26281 ttccgtattg catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt
    26341 atcaggcgga cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat
    26401 tttgattcta gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa
    26461 ttatctgcaa agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga
    26521 taaagtagtc aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa
    26581 ctattcgaaa tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga
    26641 aaagcagcaa acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc
    26701 ctctaccaca aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga
    26761 tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg
    26821 aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga
    26881 accaatcaat ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat
    26941 cgggaaatcg acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga
    27001 aacgctaggg acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac
    27061 aaacaagacg atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat
    27121 ccgaagtgct ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct
    27181 gctaagtggc ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa
    27241 ttttttgatt ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga
    27301 aaatgcattg atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat
    27361 caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg
    27421 agactatgat tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc
    27481 acaagatgag ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa
    27541 agaacagcaa aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat
    27601 ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct
    27661 ggaagatcac gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca
    27721 agaaacaaaa ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta
    27781 acgaaaaaac caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc
    27841 aaacaaaaat tccatattta cctttttcac caataagaag accaattact agtcattctc
    27901 actaaagctt ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt
    27961 ttgggcttat tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat
    28021 ctttctttca attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt
    28081 ttgtctaata gacctgattc tattaattct aataatccac aaaccatgat caccatacta
    28141 gatgtatctt tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta
    28201 tcttctggca accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt
    28261 tgttctttca aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta
    28321 gcccaacagg aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct
    28381 ttgacatcca tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct
    28441 gcattttgaa tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc
    28501 gcccaataca ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa
    28561 ggatcactag gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt
    28621 aatgtttctg cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa
    28681 tctgctacgg ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga
    28741 cagtcaatcc tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga
    28801 tcacccgtct tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca
    28861 atattgtctc ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga
    28921 ctgttactga tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta
    28981 ttccattctt tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt
    29041 atctcttcaa acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc
    29101 tttttttatt gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg
    29161 gacgattgtt ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc
    29221 cgagccaatg ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc
    29281 atgtcatcaa gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat
    29341 cgtcacttaa acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt
    29401 cttcttctat gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat
    29461 atagaacata ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag
    29521 gaataagcca agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct
    29581 ctttcttaca tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga
    29641 tgctctcatt cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg
    29701 aatccattgc catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac
    29761 tagaataagc aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt
    29821 gtcccgcatt caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg
    29881 aaagaagttt agttccttca cgaataggca taggcatttc tttagccgtc caatactcat
    29941 ggtcttcagg tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac
    30001 tattataggg ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt
    30061 tttcatcaaa tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga
    30121 tttcacctaa tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa
    30181 ctgcaaaacg ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa
    30241 aatgaatata gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct
    30301 tttctggttc tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat
    30361 aatcttggcg acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta
    30421 tattatcaaa tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa
    30481 ttgttccgac aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa
    30541 tttgcgagaa ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata
    30601 attgttcatg atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc
    30661 tttcatgtat ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg
    30721 atagaattaa tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata
    30781 aaacccgaga gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta
    30841 tataatcaat ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt
    30901 taaataagtc aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat
    30961 tttctcctct ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc
    31021 aataaaaaaa taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc
    31081 ttctgctccg cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc
    31141 tgtcttttgg agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac
    31201 atatcctgct aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt
    31261 cgaatctttt acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat
    31321 aaaatttact gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata
    31381 acttaacgcg aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt
    31441 gtatccttgc ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat
    31501 atgcccggtt gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg
    31561 agcaatttta gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa
    31621 attcatcata ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata
    31681 gttccctgaa atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg
    31741 actatgtttg ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc
    31801 tacatcatga tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg
    31861 tattgctgcc tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc
    31921 actccaaaaa ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt
    31981 tccgttgtaa gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt
    32041 ttcagaagct acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat
    32101 cccgccattc ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta
    32161 acattcgtta ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact
    32221 gcgcccacac ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg
    32281 cgccataatt aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa
    32341 ccgtatattt atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc
    32401 ccataaagat aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt
    32461 tcgtaaaaat cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag
    32521 gaaattgact gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga
    32581 tgatcacacc tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa
    32641 tcaatgtggt aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat
    32701 tccccataaa acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta
    32761 aaactgtact gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc
    32821 tcgccatgat ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc
    32881 cacttaaaat ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca
    32941 aaaatattaa ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt
    33001 gtttagtcat ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca
    33061 aacaccatcg tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt
    33121 ttatataaat tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag
    33181 aatatatttg ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt
    33241 aaaatgattg gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct
    33301 ccatctattt cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat
    33361 aaaatagtgt tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc
    33421 caaaaaggat ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg
    33481 aataacccag tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta
    33541 atggttggaa tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc
    33601 aataaaacag caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata
    33661 atcaaaacat tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg
    33721 tcaaatccaa caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata
    33781 atgatgggaa tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa
    33841 gcactttttt tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt
    33901 ggtaacgaag attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt
    33961 tacgccgtac ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc
    34021 tgtttgccaa tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc
    34081 tggtaaaaca gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg
    34141 tcctccttag tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga
    34201 tgtagccacg cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt
    34261 aagaaattgt tgtgccactt gtggattttt ggcatcttta gggacataaa aacttgatcc
    34321 tcctgatact gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg
    34381 ccatttgtca gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc
    34441 aacaaacatt gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac
    34501 aatatctgag tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt
    34561 cagacggata tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat
    34621 aaacaattct tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt
    34681 taatgctttt gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc
    34741 tatttcagca aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca
    34801 aggaataatc gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa
    34861 ctgatcgcca tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa
    34921 tttttccagc acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc
    34981 gtgtgttacc aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac
    35041 aatttttaag tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac
    35101 atagtttgtc agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat
    35161 tggatctgcg atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa
    35221 taatagggta atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc
    35281 ttctttcttt tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt
    35341 aacacaaata tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta
    35401 taatagtcat taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc
    35461 ttttgttaac aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag
    35521 atacccatct tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta
    35581 atttatatgc tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca
    35641 ttgataaaca gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca
    35701 ttatccagtt ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta
    35761 tagatcaaga aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt
    35821 atctaaaaca tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac
    35881 tatgccaaca gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa
    35941 ttcaattgtc ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt
    36001 caaatgagaa agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc
    36061 caatcatacg atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc
    36121 aaattttttt tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa
    36181 gtgttattag ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa
    36241 atgaattaac ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt
    36301 cttcagcttt taaaaaatat acagggttat ccccttcaaa gtattatcta tccttaaaat
    36361 agaaaaaaat ttttaggttt tatccacaaa ttatttctct atattttctg tctattcaaa
    36421 ataaacaata attttccggc acaattattt gctgtttttt accataacat agtacgatca
    36481 acgtagaaaa ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc
    36541 ttcatttgcc actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta tctatgaacg
    36601 cggagaaaat atttcttatg caagttgtgc tttgccttat tatttaggag gtgtgatcac
    36661 tgaccgtgac tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa acaacatcga
    36721 tgtctttact aaacacgaag taacagcaat cgatccttct actaagcgat taacagttaa
    36781 agacctatcc acaaatgaag aaataaaaac agattacgat aagttgatca tctcttctgg
    36841 tgctagacca gattatccgg atattcccgg agtttttgaa gcagaaaacg gctttgtcct
    36901 ccgtagtgtg acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa atccacaaca
    36961 tgtcgtcatt cttggtgcag gtgttatggg tctggaatta gctgagaatt tcaagcatcg
    37021 cggcttagac gtgactttaa tcgatcaatt gccacaagtc gctttccctt atgatccaga
    37081 aattgctaat ttagtttatg acaaattgct gaaagaagga ttagccgttc atttagaaac
    37141 aagagttact gagatccgtg ataaaggtcg agaaatagta ttatcagatg gctccgtcct
    37201 ttctgctgat atgctaattt ttgctgttgg tgtttctccg aagaatgaag tggtgaaagc
    37261 aaccggcata caattatcag atacaggtca gatcattgtt gatgaccagt tacaaaccaa
    37321 tctgccggac atctatgcga ttggcgatat tatcgaaacg actagtgtag tgactggtca
    37381 gccgatccaa agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc tggcagatat
    37441 tttaaatggt acgcctatgc gctatcgcgg atatattggt gcaggtgtcg caaaaatctt
    37501 tgatcataca gcaagttatg cgggaatgac agaacatgaa ttaaaagcat caggcataac
    37561 aaattataaa actgttttta tcactccctt tgaccatgcc tatttctatc cagaagctac
    37621 aagattaaat ctaaagctga tttttgatgc agatagcggt cgtattttag gtggacaagc
    37681 atttggagaa aaaggtgtcg ataaacggat gggagaactt tctgtagcaa tcaccggaaa
    37741 cttgacagtc tttgatttgc ctgatttgga gttgccttac tctccaccgt attctactac
    37801 ccgtgacccg ttgaatatag ctggttatgt cgcaatcaat caaatgacga atatcgtaga
    37861 aacgatcaaa gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct ttttagatat
    37921 acgtgaacct aataaagcac cttctggcag tatctcggca acaaaaaata ttccaatgaa
    37981 tgagttacga gatcgaatca atgaaatccc aaaagataaa aaagtttaca ttactttcag
    38041 aagaggattg aatacttata cttctgcccg aatcttggca ggtttgggta tcaaagcgat
    38101 tttgattgaa gaataaaaaa acgaccgcgg tgggtagccg cggtctagga gttattttta
    38161 cttgaggagt aaaaatgaaa aagtatatag ggttgttggt tggtatgtat atagaataga
    38221 agataaatgt gaagaaattg tgcattattt cttcacattt attaggttat agtttaagaa
    38281 agtttaaaca ttgcgcaatt taattatttt agctgtttct tttcataaaa aaacatgttg
    38341 tgactctgag acaaatcata gtccatagtg atgtctctag agtaactacg cctaatatta
    38401 cgaggcatca tattaatcta tcagcactag tacctaaact agcatccctt tcgaatattt
    38461 tttcttatat aataaagttt gctttcactc ctctaatctc accaaatcga ctggcttgcg
    38521 ttgtgtgata ctaggaactg attggtttag aacgactttt ctattgtaat catatttttc
    38581 gccatcctcg ttgtcttcta atccttcaat gaatgatccc taatgtgtgt attaaataat
    38641 ttaatttttt tgttcctatt tatttaac
//
LOCUS       DADOOS010000010        38286 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.010, whole genome shotgun sequence.
ACCESSION   DADOOS010000010 DADOOS010000000
VERSION     DADOOS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.010"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(107..982)
                     /locus_tag="J8C37_000450"
     CDS             complement(107..982)
                     /locus_tag="J8C37_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ5303912.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1091..2860)
                     /gene="aspS"
                     /locus_tag="J8C37_000451"
     CDS             complement(1091..2860)
                     /gene="aspS"
                     /locus_tag="J8C37_000451"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAZ5303913.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2875..4167)
                     /locus_tag="J8C37_000452"
     CDS             complement(2875..4167)
                     /locus_tag="J8C37_000452"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAZ5303914.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4561..5508
                     /locus_tag="J8C37_000453"
     CDS             4561..5508
                     /locus_tag="J8C37_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAZ5303915.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5597..6856)
                     /locus_tag="J8C37_000454"
     CDS             complement(5597..6856)
                     /locus_tag="J8C37_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAZ5303916.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7027..7473)
                     /locus_tag="J8C37_000455"
     CDS             complement(7027..7473)
                     /locus_tag="J8C37_000455"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAZ5303917.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7484..9697)
                     /locus_tag="J8C37_000456"
     CDS             complement(7484..9697)
                     /locus_tag="J8C37_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAZ5303918.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9930..10694)
                     /locus_tag="J8C37_000457"
     CDS             complement(9930..10694)
                     /locus_tag="J8C37_000457"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAZ5303919.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10695..11642)
                     /gene="prmA"
                     /locus_tag="J8C37_000458"
     CDS             complement(10695..11642)
                     /gene="prmA"
                     /locus_tag="J8C37_000458"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAZ5303920.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11655..12143)
                     /locus_tag="J8C37_000459"
     CDS             complement(11655..12143)
                     /locus_tag="J8C37_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAZ5303921.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12109..12780)
                     /locus_tag="J8C37_000460"
     CDS             complement(12109..12780)
                     /locus_tag="J8C37_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAZ5303922.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12918..14204
                     /locus_tag="J8C37_000461"
     CDS             12918..14204
                     /locus_tag="J8C37_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAZ5303923.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14276..14470
                     /gene="ssrS"
                     /locus_tag="J8C37_000462"
     ncRNA           14276..14470
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="J8C37_000462"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14548..14832
                     /locus_tag="J8C37_000463"
     CDS             14548..14832
                     /locus_tag="J8C37_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303924.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14905..15384
                     /locus_tag="J8C37_000464"
     CDS             14905..15384
                     /locus_tag="J8C37_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ5303925.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15606..15749
                     /locus_tag="J8C37_000465"
     CDS             15606..15749
                     /locus_tag="J8C37_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAZ5303926.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16327..17910
                     /locus_tag="J8C37_000466"
     CDS             16327..17910
                     /locus_tag="J8C37_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ5303927.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18347..19531)
                     /locus_tag="J8C37_000467"
     CDS             complement(18347..19531)
                     /locus_tag="J8C37_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAZ5303928.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19556..20563)
                     /locus_tag="J8C37_000468"
     CDS             complement(19556..20563)
                     /locus_tag="J8C37_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ5303929.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20735..21094)
                     /locus_tag="J8C37_000469"
     CDS             complement(20735..21094)
                     /locus_tag="J8C37_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303930.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21066..21437)
                     /locus_tag="J8C37_000470"
     CDS             complement(21066..21437)
                     /locus_tag="J8C37_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAZ5303931.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21454..21756)
                     /locus_tag="J8C37_000471"
     CDS             complement(21454..21756)
                     /locus_tag="J8C37_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303932.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21773..22243)
                     /locus_tag="J8C37_000472"
     CDS             complement(21773..22243)
                     /locus_tag="J8C37_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAZ5303933.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22218..22496)
                     /locus_tag="J8C37_000473"
     CDS             complement(22218..22496)
                     /locus_tag="J8C37_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAZ5303934.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22493..23542)
                     /locus_tag="J8C37_000474"
     CDS             complement(22493..23542)
                     /locus_tag="J8C37_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAZ5303935.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23499..24428)
                     /gene="cpaF"
                     /locus_tag="J8C37_000475"
     CDS             complement(23499..24428)
                     /gene="cpaF"
                     /locus_tag="J8C37_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAZ5303936.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24560..26314
                     /gene="ade"
                     /locus_tag="J8C37_000476"
     CDS             24560..26314
                     /gene="ade"
                     /locus_tag="J8C37_000476"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAZ5303937.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(26412..27074)
                     /gene="trmB"
                     /locus_tag="J8C37_000477"
     CDS             complement(26412..27074)
                     /gene="trmB"
                     /locus_tag="J8C37_000477"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAZ5303938.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(27144..27923)
                     /locus_tag="J8C37_000478"
     CDS             complement(27144..27923)
                     /locus_tag="J8C37_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAZ5303939.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(28130..29341)
                     /locus_tag="J8C37_000479"
     CDS             complement(28130..29341)
                     /locus_tag="J8C37_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5303940.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(29334..30071)
                     /locus_tag="J8C37_000480"
     CDS             complement(29334..30071)
                     /locus_tag="J8C37_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303941.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            30209..30643
                     /locus_tag="J8C37_000481"
     CDS             30209..30643
                     /locus_tag="J8C37_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAZ5303942.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            30636..30980
                     /locus_tag="J8C37_000482"
     CDS             30636..30980
                     /locus_tag="J8C37_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAZ5303943.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            31160..32170
                     /locus_tag="J8C37_000483"
     CDS             31160..32170
                     /locus_tag="J8C37_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ5303944.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(32239..33183)
                     /locus_tag="J8C37_000484"
     CDS             complement(32239..33183)
                     /locus_tag="J8C37_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ5303945.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(33167..35887)
                     /locus_tag="J8C37_000485"
     CDS             complement(33167..35887)
                     /locus_tag="J8C37_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5303946.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(35884..37104)
                     /locus_tag="J8C37_000486"
     CDS             complement(35884..37104)
                     /locus_tag="J8C37_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAZ5303947.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(37385..38230)
                     /locus_tag="J8C37_000487"
     CDS             complement(37385..38230)
                     /locus_tag="J8C37_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5303948.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        11297 a         7938 c         6699 g        12352 t
ORIGIN      
        1 ataaaaaagc agtttttctt tgctgattct actatggtag aaaaaaactg cagacaaacg
       61 aacgtctgtc tacagcagta tttcatgaat ttttgtcaaa ctcgatctaa atgccatcct
      121 catagaaacg accaagtatc tggacgtttt ctgcaatact tacaaaagct tgtggatcag
      181 aagctttcat tgcttctcta agtagaggca gttcgtatcg tgtaacgatc gtcatcaata
      241 ctgtctgctt atcatgctta taagcacctt ctgcttcatt gatgatcgtg atccctctac
      301 gcatacagtt ttggatttca tttactactt tttctggatg tttcgttacg atcattacct
      361 gcatcttctt ttgtttagta taaacgacat cagttacttt tccactgacg aaaatagaca
      421 acgtactgta aaacatatat tgccagccaa atagaattcc ggcaacaaaa acgatcaatg
      481 cattgaagta gatcgaaata gaaccaatcg actttcctgt ttttttacga atcgtgatac
      541 tcacgatatc tagaccgccc gatgataggc cgtttctcaa cgctagccca atacctagtc
      601 ccatcacagc ccccccaaac aacgcacaga tgattggatc ggtagatagg actgtttccg
      661 gtaaaatatg gatacaaagg gaggtcaaag tgactgtgat aaaagtgaaa attgtaaact
      721 tcttcccgat ttttaaccac gccaatacaa acaaaggtat attcagtaaa tacaaagttg
      781 ttgataccgg tacttcgaat cctatcagtg attttgacaa agtcgtcaaa atctgagcta
      841 gaccagtaat accacttgaa taaatatgtc ctggctgata aaagaaattc aatgcgactg
      901 atgcgatcaa cgcataaacg atagataccg aaagcttggt cgtgtaatca tgataagccc
      961 aattttcgaa tgtttttttc atggaaaagc cccgctttat atgttatggc ttcattataa
     1021 agtgtttttc aattaaatca aggttgtcaa aagaagcgta ttcgacaaat cctttttacc
     1081 tttttatcgg ctattcttct atattcgtta catcaatatt caattcgaac aattgtaaag
     1141 gcgaaaccat acttggtgca tcactcatca catctgctgc ttttccgttt ttaggaaagg
     1201 caatgacttc acgaatatta tcttctccgg ctaataacat tgcaaaacgg tccaatccta
     1261 atgcaatacc gccatgtgga gggaatccgt aatctaaagc tgttaacaag aaaccaaatt
     1321 gttcttccat ttgttctttt gtaaagccta gagtttctag tactttttct tgaagctcac
     1381 gagtatgaat acggatagaa cctccgccta attcataacc gttcaagacg atatcataag
     1441 cttctgcata gacttttgca gggtcttcag ccaataacgg gatatcttct tcttttggca
     1501 tcgtgaatgg atggtgagca gagacatatc gaccttcttc ttctgaaaat tcaaattgtg
     1561 gccaatcagt tacccataag aaattgaatt tcgattcatc aattagtcct aattcttttc
     1621 ctaaacgtgt ccggatagca cctaatgcag cagcgacgat ttcactttta tctgcgccaa
     1681 acatcagcaa gtcacctggt tttgcatctg ttgctttaat gattgcttct gttgcttctc
     1741 ccatgaattt agcaattgga cctttcaatc cgtcttcttc tactttcagc catgcaagcc
     1801 ccttagctcc gaattgtcct acatattgac ctaactgatc catgtctttt cgtgagtagc
     1861 gatctgcagc gcctttagca ttcaatgctt tcacgacacc gccgttttca agagccattt
     1921 ggaagacttt gaattctact tctttgactg tatcgcttag atcgatcaat tccatatcaa
     1981 aacgtgtatc cggtttgtcg ctgccgtagc gagccattgc ttcatcatag gtcatacgag
     2041 gaaatggcaa tgtgacttcg ataccgcgaa cttctttcat taccttcgca atcaatccct
     2101 ctgtgtaagt ttggatttct tctgctgtta agaacgttgt ttcaatatct acttgcgtaa
     2161 attcaggctg acggtctcca cgtaaatctt catcgcggaa acaacgaacg atttgataat
     2221 aacggtcgaa tccagcattc atcagcaatt gtttaaacaa ttgaggagat tgcggcaacg
     2281 catagaaatg tccagcatgg acacgtgaag gaactaggta gtctcttgcg ccttctggtg
     2341 tagatttccc taggtaaggt gtttcgatat cgagaaagtc attatcatct aaataatgac
     2401 gaatcgcttt tgttgtcgca tttcttaatt tgatgttttt agtcattttt ggacgacgca
     2461 gatccaaata acgatatttc aaacgtattt cgtcactgat tgcttgatca tcttcaattt
     2521 gaaatggcgg ggtttttgct tgattcaaaa tcacgatttc acttgcaatc acttcgaatt
     2581 caccagtggc cattttagga tttactgcat tttctccgcg tcttctaact tccccagtca
     2641 cttcgatcac gtattcactg cgacatttat ccgcgatttc ccatgcttct ttgttgattt
     2701 caggattaaa gacgatttga acgattcctt cacgatcacg taggtcgata aagattactc
     2761 ccccaaggtc tcgtcttttt tgtacccatc cttttaatgt aactgtttgt tctaattgtt
     2821 gttcggaaac taatccgcaa tagatcgttc tttgtgccat ttattttttt cctcctactc
     2881 gaataaagtc atttcatcat agacttcatc aaaatgttca tatatctctt ctaatggaaa
     2941 acttttttct tttcggcttg ccattgcttt gacattaatc gtttgactag caagctcatc
     3001 ttcaccgatc accaatacta atttggcatt cgctttatct gctgtcttaa attgcgcttt
     3061 tgctttacgg ttcatgaagt cgcggtcagc tgaaaaacca aagtttcgaa tcgcttggac
     3121 cactttcaat gcttcgatat ttgtttcgtc tccgagtgcg accacgtaag catccaactc
     3181 attaaatgca gggataacta cttcctctgc ctccatagtc aagagtaatc gctcgatacc
     3241 cattgcaaat ccgaagccag gtgttgaagg accacctagt tcttccacta gattgtcata
     3301 acgtccgcct gcgcaaatcg ttgcttgtgc ccccattttt ggtgcgtcac tcatgatttc
     3361 aaaaatcgta tgtgtgtaat aatctagtcc gcgaaccata ttactgtcga tttcataagg
     3421 aacagccaat gcatccagca tggaaacaac ggtatcaaaa tgtttttgag caggctcgct
     3481 tagataatct aagatagatg gagcttctgc cacaaatttt tgatctcgtt tgtctttact
     3541 gtccaatacg cgtaatggat tttcatgaag gcggcgttta gagtcttcac tcagctctga
     3601 ttcaaaaggt aaaagatagt cgatcaatgc ttgacgatag gcttgacgcg tttctttatc
     3661 acctaatgaa ttgatgacta gccggatttg cgtgatcccc aactgtttga agaaatcaag
     3721 tgccatcacc atgctctcca catcagttgc agggttttct gatccaaatg cttctactcc
     3781 gatttgatgg aattgacgta accgtccttt ttgagggcgt tcgtagcgaa acattggtcc
     3841 catataaaaa gttttgaacg gtttcgcata ctctgggccg aacaatttat gctcaacaaa
     3901 agaacgaacg attggtgctg taccttctgg tcgaagcgtc acatggcgtt cccctttgtc
     3961 ataaaaatca tacatctctt ttgaaacgat atccgttgta tcccctacac ttcgggatat
     4021 cacttcatag tgttcaaaaa tcggtgtacg catttcatta tactgataat ctctgaataa
     4081 taaacgtgct gtttcttcga caaactgcca tttttctgat tcgcctggca aaatatcatt
     4141 tgttcctttt ggtctttgga aactcatagt tttctctccc tttcttagct atgtaaataa
     4201 aaaaatcacc cttgttcatc atgaacaagg gtgaatgaaa ttcatacacg gtaccaccta
     4261 attttgaaag atttctcttt cctcttacga ataacgctcg tacgcgggac ggcttttcac
     4321 tcgtccatct ccagagtgtc tttaggctaa tgaaacttta gatcattttc agcctcgatg
     4381 atcctctctt gtaaagtttc aaaaacttac tcgctctttc attgacattc gatattactt
     4441 ataacctact aaaaaccagt ctcaaagtca agagatatct gactatttcc cctcacaaaa
     4501 acgttttagt tcagcttttt ggtattttgt tttagactag acaaaaagga ggaatcagct
     4561 atggcagaaa taagaatcgg acattcacaa gtctacgcag aacagcttgg attaggagca
     4621 aatgccgttg gaggacataa tctttttgac ggtttggaag atgagacagg aaaacaagta
     4681 gttcgtacag ctttgaatag cgggatcaat ttgatcgata ccgcttatgc ttacggaaac
     4741 ggccgttccg aagaactgat tggcgaggta ctgaaggaaa aagaatatga tcgctcacgt
     4801 gtcgtcatcg ctacaaaagc tgctcatgta ccaaataaag ggcgaacatt tgataattct
     4861 cctgagtttc tcaaacaatc ggtggaagat gcactaaaac gtctgcaaac tgattacatc
     4921 gacatttttt atatccactt tccggatgaa agcacaccaa aaaatgaatc agttgctaca
     4981 ttgcacgaat taaaagaagc aggaaaaatc cgagcagtcg gtgtgtcaaa cttcacgtta
     5041 gaacaattga aagaagcgaa tgcagatgga tatgtcgatg tagtagaaga taaatacagt
     5101 ttgatccatc ggcaggctga aaaagaatta ttcccttatc ttgaaaaaaa caaaatcagt
     5161 tttgttccct attttcctct tgcttccggt ttgctgacag gaaaatatga acttggtgag
     5221 gaaaaacaat ttggtgaagg agatcctcga aaaagaaatc cagatttcca aggagaacgt
     5281 ttcagagaaa ttttaaccgc tgtcgatgta cttcgcccca ttgctaaaag ataccaagcc
     5341 actcctgcgc aacttgtttt agcttggtac atgaagaatc cacgagtgtc tgtagttata
     5401 cctggagcta agcgtccaga gcaagtatct gacaatgtcc aagctttaga tttgcattta
     5461 tccaatgaag attatcaaac cattgatgaa gcatttcgtg gattttgatc aattgacctt
     5521 atcaatttat agtaatatga aaaaaggaag atcagcactg tttgatcttc ctttttttcg
     5581 ataagtttat gctaatctaa ttcttgtaaa gtagcgctcg gctattcgga taagtcggaa
     5641 actgattgaa tggaatcgtc caatcttgtt caggtacaga gaactcaaac ttatttacta
     5701 gctgatcgga aaacacacgt agactatgaa gagtgatcca ttctcctgca cagcgatgca
     5761 tttgtcgaaa atttcccccg ccttgtgcaa tcatctcata ttcttcttta taggaaatat
     5821 cttttgcttt ccctacatac cgtttgatca taaaggaatc tggcgcttca actgttcgtt
     5881 catcatggtc tgttccatat aaatctaaaa taacccaact gccttctggt atacgataac
     5941 catctacttc tacttctttc agcgaaatcg caggtagcat tgggaagaaa ggataatagc
     6001 gtctcatttc ttgaataaac ggatcttgta acgtcgaaaa gtcatttttt aattgctgat
     6061 acaaatctgg tctactgaaa agtgcatgtc ccatcaatgc cgcccacaca gttagagcaa
     6121 ccgttggacg gatgatgttc aataattcaa cagctgccac ttccagaggt aaaagctgac
     6181 catctaaatc tgtggcattg gcaaaagcat ataatgccac attttctttt ccagccacag
     6241 gattttgtcg agcttcttca atcaagcctt gcgcccattt ttctgatttt ttgcgattct
     6301 cgacaccttt tatgtgatca atcggagaag taaacgttcc gctgatcata gagatttgat
     6361 attctgctaa tttttcgacc tcatctttag aaatcgctga taaattgata cctgcccact
     6421 cgcaaataga tgtaaagaaa accatcttac ttaaatcaaa tagttcaaat tgaccatgct
     6481 gtgcttcaag tgcttgtgtc agattcttat ctaagatacg atgataatct tccattcttt
     6541 ccggtgtcat tagatccata aaaatatttt tccgatggtg atgagcagct ccatccaaag
     6601 tctgtactcc accttgacca aaaagtgtct tcaatactaa ttttggcata gcaccttttc
     6661 gtttgaaatt ccttggatca taaaatactt ttgctgctga tgatccgtag atcgtgatag
     6721 cttctttatt gaaaatcttt gctttgacga cgggagcgtc cgcttcatgg cgtaactcct
     6781 ccaacatgtt gtatcctttt tgatacaatt tttttaaatc tgtgattttg atatctacta
     6841 ctggtacttc tttcatcttc agcaacctcc tataaaaaat aacttgtata atttaagctt
     6901 aacttttttt ccgactgctg acaaaagaag cgctttttgc agacaaaaaa gccacttaag
     6961 ttagagttta tcagtctaac ttaagcgacg tgtttcatag tttttactag tgaaaaccag
     7021 attactttat ttgtttttag aatcaataat gatcgttaca ggtccatcat tgacgagaga
     7081 aaccgacata tctgctccga attgtccggt agcgactgtg atgccgcgac ttctgattcc
     7141 ttcattgaat gattcgtata aaggaatagc tgtttctggt cgtgcggctt ccacaaagct
     7201 tggtcgattt ccttttttcg tatccgcaaa cagtgtgaat tgagagatac ttaaaatttg
     7261 tcctcccaca tcttctacac taatattcat ttttccttgt tcatcttcaa aaatacgcat
     7321 ttgtgcaatt ttttttacca gatagtccac atctttttgc gtatcggttt catgaatgcc
     7381 taataaaaca agtaggccgt agccaatttt tccaatttct tgctgatcga ctgacacagc
     7441 agctttactt acccgttgaa caactgctct catatctgtc ctcctatcca ttcgtccggc
     7501 gtacactgta gacatcaggt actgctttga ttttatccac gatactcttt aggtgggata
     7561 aattctggat accaaccgtc aaatggatcg tcgccatttt atctttattc gtccgtgctt
     7621 caacgctgtt gagatttttc gttagcgcat tgactgtttg aagtacatca ttcaataagc
     7681 ctgaacgatt atagccgtaa atttccaaat ctgcatcata ctcttttcgt gtattcgatg
     7741 tatcttccca ttcgacttca atcaaacgtt ctgctacatt tggtttgtca ggctgaacat
     7801 tcggacaatc tcgacgatga atggatatcc ctctgccttt agtgatataa ccaacgatat
     7861 catcaccagg aatcggatta cagcagcgac tgatacgaat tagtaagttt tctacccctt
     7921 gaatgacaac gccaccttca tgacgtactt tcattttgtc aggttctttt ttcggctgat
     7981 tcatgatttc ttctgcttct tgcttttgct gctcgatttt ttgttcttta cgttcttttt
     8041 cagtcagacg attggccatc gtcaaggggc taacttctcc ataccctaca gctgcataga
     8101 gatcatcttc tgtttgataa ttaaaacgat cgagtgcttc ttgcagtttg ttcttcgtca
     8161 aaatatcttt aggcgtaaat cctagatctg taatacattt gaccacggat tcgtggcctt
     8221 tgatcacatt ttcttctcga tcttgggctt tgaagaaacg tttgatctta tttcttgctt
     8281 tgctagtagc aactaatttc aaccagtcgc gacttgggcc aaatgaattt ggagaagtca
     8341 tgatctcaat gatatctccg tttttcaatt tgtaatcaag ctgcaccatt ttgccattta
     8401 cttttgcacc agtggttttg ttaccgatat ctgtatggat actgtatgca aagtccaatg
     8461 gtccggatcc ttttggtaac tcagtaacgt ctccttttgg tgtgaagacg tagactttat
     8521 cactaaagat atctcctttt acgccttcca taaattcaga agcatcatag ctttcgtctt
     8581 gaagttcgag tatctcatgg aaccagctta attgtttcgt cataccatct ggttctactt
     8641 tttcattttt tccttcttta taggcccagt gtgcagcaac cccgaattca gcgatttcat
     8701 gcatttcctg tgttcggatt tgaatttcta ccggatttcc tgccggaccg atcacggtag
     8761 tatgcaaaga ttggtacata ttagcttttg gcatggcaat ataatcctta aatctgccgg
     8821 gcattggttt ccattttgtg tggattgctc ctaatacagc ataacaatcc ttgattgaat
     8881 ctacgatcac tcgaatagct agcaagtcat aaatttcgtt gaactgcttt ttctgatctt
     8941 tcattttacg atagattgaa taaatgtgtt ttggccgtcc atagatttct gcaaaaatcc
     9001 ccagctcttc tgtcgctatt cgaatatctt caacagtacc acttacgtat ttttcacgtt
     9061 cttctctctt cgtctgcatc aagtggacga tacggtaata ctgttttgga tttagataac
     9121 gaagtgctgt atcttctaat tcccatttga tccggctgat ccctagacga tgtgcaagag
     9181 gcgcatagat ttctaaagtt tcctgagcga tccttctttg cttatcttct cgcaggtgct
     9241 tcaacgtacg catgttatgt agtctatcag ctaatttgac catgataact cgtaaatctt
     9301 gagccattgc tagcagcatc tttcggtggt tttctgctag ctgctcttcg tgggatttat
     9361 attttatttt ccctaatttg gttacgccgt ccactaacat agcaacgtca tcgccaaatt
     9421 cttctctcag atcttctaaa gtgatttctg tatcttcaac aacatcatgt aagaagcctg
     9481 tagccactgt atggggatcc atttttaaat ctgctaatat acctgcgact tggataggat
     9541 gaataatgta gggttcgcct gattttctaa actgtccgtc atgtgcagca gtcgcgtatt
     9601 cgcatgcttt ctcaacaaat gctacatgtt cgggacccat gtattgacta acgagcttta
     9661 tgacaccggg tcccgttaaa atttcttctt ttgtcacagg aatcgatccc cctttctttt
     9721 ttcggtaaaa cgggaatcgg gacaaatatc tgtttgataa ttgtcccaaa atccgtgcag
     9781 ttatacgttt ttttacggaa atgttcgctt tttaaaagct ttttcaaacg atttttcgta
     9841 tccattgtat aaataattgt ttctattata caaattgttg actatttttt caattctttc
     9901 tgtcgatact cttttattaa tgaagaacgt tagctgaaag aaggattcat taattcccat
     9961 tgatagctag cagcacttaa taaataaaga ggtgctgttt ctgtacgaag gattcttggt
    10021 cctaatccac aaagtatccc tttgtgttct acaaaataat cgacttcatc gcgggataat
    10081 ccaccttctg gtccaaagac agctaagatt cgactatctg gttttgtttc actaagaatt
    10141 ttggctgcat ttgatttttc gccttgcttt gctgattcct cataagcaac caagaccacg
    10201 tcataatcat ctagccgctc aaataaatct tgtttcgttt ccaataactc tactgatggc
    10261 acgatctgcc gatgggattg ttccgctgcc tcttgagcaa tcttttcaag ccgttgttgt
    10321 cgtttcatac gtttttttgc atcccattta acgacagagg ctgatgccgg gaaaccaaga
    10381 aaagcttgcg ctcctaattc agtggatttc tgaacgatcc aatccagctt ttccccttta
    10441 ggatagccac tcgctatggt aatcgaactt ggcagttcct tttgattttc ttctttttta
    10501 atttctttca gataaacagc ctcttcagta atctccgtga tttctgcaac aatggctatt
    10561 tgatcattga atgccaaaaa aacttcatcc tcgggttcca tcctcataac ccgtacaatg
    10621 tggtgatagt tttccccttc taaaacaaaa tagtcttttg attcatatga ttcttttaaa
    10681 aaataccgct gcatctattc cacctctggt tttttcaaaa taattgcata ccagtctttt
    10741 tgctggaaaa tttgatccac gataaaccct tgttctaaca tggcatccag aaccatttgc
    10801 ttcttctctt caatgattcc tgaaacgatc agcgtgcctg tttgcttcag taaacgccaa
    10861 gcatctggga tcatcaataa gatgatatct gctaaaatgt tcgcaacgat tacatcactt
    10921 tcaatctcga ttcctgtcaa aagatcgtta gctgatacat ggacatcttt ggctatttca
    10981 ttcatatcca tattttcttt cgccgaacga acggctactt cgtctaaatc aaaggcatag
    11041 acatcttttg caccaagata ttttgctgca atacttagca cgcccgaccc agttcccaca
    11101 tctagcaatg tttcaccacc acgcaagact gtttctagtg ctgtcaatgt caaggaagtc
    11161 gtgggatgag tgcctgtgcc aaatgccata ccaggatcta gtgtgataat tttttctaga
    11221 ggatcttctg tctggtagtt ttcccagctt ggcgcaaccg tcagcattcg cgtaatacgc
    11281 accggatgat agtatttctt ccaggctgtt gcccagttgc tttcctctac ttcactaact
    11341 gttactttat tttttccaat agctagacca aactcaggta attgtgtgat acgatctttg
    11401 ataaaaggca agatttccgg taagaaagtc gtttccggaa aataagccat gactagtgcg
    11461 ccttctttca gcgatgtaaa atcattttta tctaaaatct caccatattg atccgactga
    11521 aaatgttcaa catctaaaga atcttcaatc gcgacaccac tcgcccctgc ctccatcaaa
    11581 atattagcaa ctgcttcgac tgcttcactt tcagtttcta cttttacttc attccatttc
    11641 atatctgtca ctccttaata acttgggtac ggtaaataag tatctgcttc attttgctgt
    11701 tttacttcta ctaattctgc tagttgttga ttatcccagt gaagctcgaa tacagcttcg
    11761 tcatcgtctt catccaaaaa atcaagcaaa tcgctttgcc cttgagctaa tacttcattt
    11821 agatattcca ctaaagaatc agctactgat tttgccaacc ctttttttcc ttcataaggg
    11881 attactgcta gataattgcg ttcatcaaaa acagatttct gaggattaaa aagaagtacg
    11941 ccgtcttcaa attcgataat tggttcttgc gaaacgatcc cttctgcatc atcaatctca
    12001 aattgtgctt tattttctgc aaacaagcga acaacgatct cgatcgtatg attttttgta
    12061 tcccaatcta aagctacatc atagtctgtc acttgttttt caattatttc atctaaatat
    12121 gttaatagat ccttcttttt cattttcttt cctccaaccc caatctttct cgatttggtt
    12181 ttttctttgt aaagagatat aaggatttcc cgacacaaca aaacgcaacg gcttttctgt
    12241 ccatacacct ttgtttggga taccgattct aggcaaagca ttgatctttt ttggtgtctt
    12301 ttttttctca aaaactaagc gtaacgaact atctgctata gactgaccat aaagttcttg
    12361 aggaattgct aatgcttcca ctaattttcc cggaccgttg ctgatatctg gaccagtttt
    12421 tccgccacga tttttactca tttgatcaat cattgcggcg ggttcgattg cacgtatcat
    12481 tactccttga ggaatccctt ctggctgagt aatgatgttc aaaatccgat gagtatgcat
    12541 cgtgtaaagg tagatcgtac ctggtttttc atacattgcc cgcacacgcg gtgttcttct
    12601 taaaccatag ctatgggcgg cctcgtcttc tggacctagg tatgcttccg cgtctacaat
    12661 atatccagct aatcttcctt ctggtgtgtc atgctccaaa tacatcccca ataaatcccg
    12721 tgccacttct ggcgtcgttt tattccaaaa aaattcatct atcttttgtt tatccgtcat
    12781 ttttctcacc tactttatca tacacaatat tagttggata agctatgaaa acaaaacaac
    12841 agaaggctgt tctttctagg catcttgcta aatatgatat aattaaggga cgtaaaaaca
    12901 agaaggagga aaatcctatg caacagccat tggcttatcg catgagacct cgaacgatcg
    12961 atgaagtcgt tggtcagcag catctagtcg gggaaggaaa aatcatacgc cgcatggtcg
    13021 atgctcgcat gctctcttcc atgattctat atggacctcc tggtactgga aaaacgagta
    13081 tcgcaagtgc gatcgccggc tctactaatt atgcatttcg catgctaaac gccgctacag
    13141 attcaaaaaa ggatctacaa gttgtagcgg aagaagcaaa aatgagtggt acagtgatcc
    13201 tcttattgga tgaagtacac cgattagata aaacaaaaca ggattttctg cttcctcatt
    13261 tagaaagtgg ccgaatcatc ttgatcggag cgactactga gaatccttat attaccatca
    13321 atccagcgat ccgcagccgt acacagatct ttgaagtcaa accattaaca gaacaagaca
    13381 tccagttggc agtcgaacat gctttaaggg ataaagaacg cggccttgga caacaagcaa
    13441 tccagttaga cgaagatgct cttcttcacc tttcgcgtgc aacgaacggc gatttgcgga
    13501 gtgcattgaa tgggctggaa ctggctacac tatcgactcc ttcagataaa gaaggaagga
    13561 ttcatcttac actgtcgatc attgaagaat gtgtccaaag aaaagctctt acccatgaca
    13621 aaaatggaga tgctcattat gacgttattt cggcttttca aaaatcgatt cgtggaagtg
    13681 atgtggatgc tgcacttcat tatctcgccc gtttagttga ggcaggtgat ctggcaagta
    13741 tttgccggcg tctgatggtt atcggttatg aagatatagg tttaggtaat cctgctgctg
    13801 cggctcgtac agtgaacgct gtattagctg ctgaaaaatt gggattgcct gaggcacgta
    13861 tcccactagc agacgtcgtc gtcgatcttt gcttgtcgcc aaaatccaat tctgcctaca
    13921 tggcattaga tgcagcactt gctgatattc gagagggcaa agctggagat gttcctgatc
    13981 atttgagaga tagccattac aaaggtgcga aatcactcaa ccgtggcgtg ggttatcagt
    14041 atcctcatca ttttgatcag gcttgggtca atcagcaata tttaccagat aaactgaaga
    14101 atgctcagta ttatcagcca aaagatacag ggaaatatga acaggctctg ggtcaacagt
    14161 attaccgtat aaaagagtgg aaaaagcatc ctccaaaaaa ctagacaaga aaacggttct
    14221 gtttctgcga gctctctcat tgctcttttt aaattcatat gctactatag aggtgggaaa
    14281 tctgtgatgt tcgaattgtc ctctttgtgt gttgaccgaa catttttatt gatatcttgg
    14341 gacccgtctt gtgtcactgc tggtaacatg ccttatcatt tggtagttcg aagctacgac
    14401 catgggaccc acctgcttga attcgcggga tcaatactac aggacaaaca acggcattaa
    14461 cggagattac catcttttaa agaataaagc aatttgctct tggcaaattg ctttttctat
    14521 ttgttgctta ttttggagga tttgttcatg attaggattt taatgattat cgccacttta
    14581 gtattattat tcgttagcta ttatttattt aataaacagg atatcttttt tgtattgatc
    14641 aacaaaaatg ataaaaatca aggattccta caattttacg gtgctgcata tgctgtttta
    14701 ggcgtaatgg gtattttaac agcttttttc aatcaacgct ttattgcctt ggtcttttta
    14761 ctgatcgtaa ttattgtttc tgccaccttc agtatccgat ttgcaaaaaa aattgctgaa
    14821 ccaaagcagt aaataattct ctttttccct aaagtgttct acaataaaag agtacaatac
    14881 gattgacttg aggagtgaat gcttatgtta caacaataca aaaaaatcat ggttgccgta
    14941 gatggttctg atgaagctga attagctttt aaaaaggcgg tcaatgtagc tatccgaaat
    15001 aacggagaac ttctattagc acatgtcatt gacacacgtt ctttccaaac cgtttcatcc
    15061 tttgatggaa tgcttgcaga acaagctact gaaatggcaa agcaaacatt ggcagattat
    15121 gaaagcaatg cgaagaaagc tggcttgaat aatgtaacct ctgtcgtaga atatggctca
    15181 ccaaaacaaa ttattgccag agaaatccca gaagataatc aagtagattt gattatgtta
    15241 ggagccacag gattgaatgc tgtagaacgt ttatttattg gttctgtttc tgaatatgtt
    15301 attagaaacg cagcttgcga tgtgcttgtt gtccgaactg atttagaaaa ccaattgcca
    15361 gctaaagacc acaccaaagc ttaacacgta gacgacctgc caactatcgg caggtttttt
    15421 tgcacaaaaa accgtgataa aaacaaaaga cgcggcaagc actcatcggt ctttataatg
    15481 tttaaaataa agggaaaaca gtcaatgagt tcaagtaaaa taaaaaaagt gaacaccagg
    15541 gcgattagtc gccctggtgt ggtttaaaac gacctaagca tgccaaagct tattggttcg
    15601 tcaatgtgga agtttccgat gaatctgata cgaaaaaata tgaagtatcg atggcggata
    15661 ccgattttat cgccaaacta aaattttatt tcgggttcgg gtaccgcgag aaggcaagtg
    15721 ctacattcgg taaatcattc aagaattgat gaaaggctac cgcttgccat tgcaaccaca
    15781 acgatactcc tgaacgcgat gttggtgaga tttccaaaga gaagagctgt ttaatgcaac
    15841 ggtattaagc aaatggcaaa aggaagtcat gtagacaaag ttttttatca aacaccatat
    15901 gatttcgcct gaacgctggt ttagctcaga atacaatgat gtggtccacg agacattacc
    15961 ctcagtcaga taaaaaaagc tctctgacag aattacacat cgataaatat taagtaaatg
    16021 tcagcgctag ttgaactttc tgacactttc aggtacactg gaaaagacaa ctacacgaag
    16081 gagggaaaat actatgatgc gcatgactaa aataacaagt aatgaaaatt accaacgata
    16141 ccatatcgca tttagccatg caattggttt tacagttgct gttgacggga caagtttgtc
    16201 caaaacagtt gaattcacgt aaaatcaatg gccatctcaa ggtatttttc tcatgataat
    16261 ctgagtttaa ataacacagg ccagggataa ccatggcctt tttgagtggt ctcaaggaga
    16321 gagatcatga tcatacaagc aatttctatt aaaaaaacaa tcggcgggaa tccattattt
    16381 gaggatttat cctgtcaaat tgaagctggt gaaaaaattg ccttagtcga tatcaacgga
    16441 actggcaaat caaccttttt acaaatcttg accggcagag aaggtgtcga cagcggtgtt
    16501 atcagtcgca aaaaaggctt aaagatcggg acggtcgaac aagaacttac agtttccgaa
    16561 gcgaccgtca accactactt gctccattct gccgctgaga tacaggacct caaacagcaa
    16621 atgagtcgct acgaagcatt gatgacagaa ccagatacaa acttagagaa gtgtctggcc
    16681 cgttacggtg agctgcagca tcgctttgaa gaactaggcg gttacgtatt agaagaccgg
    16741 attgcagcga tcttgcaagg cttagggatt ccacactgtc aggaagcacg gttaacagaa
    16801 cttagcggag gcgaacgagt taaagtcgct ttagccaaaa ttttagcagc cgatgccgat
    16861 ttacttttac tcgatgaacc gaccaatcat ttagatctta acagtattcg ctggttggaa
    16921 aactatttac agaatacaaa aaaatcggtt ctggtcgttt ctcatgatcg gcaatttctc
    16981 gatcaagtca ccacaaaaac atgggaactg gaagaaggtg cattgattga gtatcccgga
    17041 aactacagcc gttttcgagt cttgaaagaa gctcgcttgg ctgagctgac aaaaaattac
    17101 gaactgcagc aaaaagaagt ccagcggttg aaagtcatga ttcgtcgttt tcgtcagtgg
    17161 gcacatgaag gagacaatga gtccttcttc aaaaaagcca aagaactgga acgccgcctg
    17221 gcgaaactga cactcgtcaa accgccacct cccccgaaaa atcggctgca aagcttgtct
    17281 aatggtggaa aatctggcaa agaagttttc atcattcaaa acttgcacca gcagtacgct
    17341 gatcaagtac tattcaaaga cagcagtttt gcagtctatc gtggagatca tttagccatc
    17401 atcggcgaca atggtgcagg aaaatcaacc ttactcaaat tattactcgg tgaggaaaag
    17461 ccttacggtg gcaccatcaa attaggtagt agcttgcaaa tcggttattt gccgcagcag
    17521 ctgcagtttg ccgatcccga tagccgtttg ctggcttacg ccatcacatt gactggaaac
    17581 gaagaagtcg ctcgacggca attagccaaa agtggctttt atcaaagcga tgtgggtaaa
    17641 cggatcaaag atttatccgg tggtgaaaaa atccgtttgg cgttgctgaa actcttttta
    17701 gaaaaaatca acgtcctgat tttggatgaa ccaaccaatc acctcgacag ctatgctcga
    17761 gaagaaattg aagaaatgct gcaagactat caaggaacgc tcctagcagt cagccatgac
    17821 cgctactttt tgcagcaaca cttccaagaa gcactcgtta ttgatcagga gcagatcagc
    17881 cgccagccgc tcggccaact gattgactag gacgtttttt cgcaacaaaa aacttgattt
    17941 tcatacctgt gctttcacga ttcaggcaaa gtacaggtgg aaaatcaagt ttttattatt
    18001 cgatcaatga atgtttcgga tcggttttct agctaaacat aaagcgaaga ataaagtcga
    18061 cagcccacga ggctgatcta aaaatgtttt ttctttttac atatgtaatg tcccttataa
    18121 agatgaatga aaataaaata ttgggatttt cctgaaaatc aataaataaa atgccagtct
    18181 tttccctttt gaccactttt ccaagcaaaa ttccctaaga agatcctgta aaaaagaagg
    18241 gaaattgaag ttaaattacc attttgcggt cttctccgca aaaaaaacac atgcaagaat
    18301 tccctcttgc atgcgtttca tcttttatcc taacgaatgc tttggcttag ccgcgcagac
    18361 gctccacatc acgggcaatc attagttcct catctgttgg gatcactaat actcttactt
    18421 ttgattcttt tgtagagata tctacttctt caccacgcaa ttcattcaac gctggatcga
    18481 tttcacaacc aaaccaagac atacctttga tgatttcact acgtacatgt ccgtcattct
    18541 cgccgatacc agcagtaaag acaattgcat caacaccatt catcgttgtc acataaccgc
    18601 cgatgtattt tcggatgcgg tccacgaaaa tgtcataagc taaacgaata tgttcttttt
    18661 catagttctt ttcaagatca cgcatatcac tagacaatcc tgttaggcct agcagaccag
    18721 atttcttgtt caaaacattg atcatttcat caatatcaat ctctaatttt tccattaaat
    18781 atgggagaac agctggatcg atatcacctg aacgtgtacc cattgtcacc ccagctaatg
    18841 gtgtgaatcc catagaagta tccatggatt tacctccatc gactgctgtg atcgatacac
    18901 catttcctaa gtggcaagtg atgattttta gttcttcgat cggtttgttc aataattttg
    18961 ccgcacgttc cgacacataa cgatgacttg tgccatggaa gccatatttt cttactttga
    19021 aatcttcata ataagacacc ggtaagctat ataaataatt ttgctttggc attgttgagt
    19081 ggaaagatgt atcgaataca gccacactca aaatgtcagg taaaatcttt ttaaacgctt
    19141 tgatccccat taagtttgca ggattatgca aaggagcaaa ttctgctaat tcagcaatgc
    19201 ggtccattac tttctcatcg atcactactg aatccccata agtttctcct ccgtgaacaa
    19261 cacggtgccc tactccggtg atttcatcat aagagcttaa aatttgcagt tcaatcaact
    19321 gatctaacag catttttact gctacttcgt ggttttcgat atccatgatt tgttcgaatt
    19381 tttgattatc gccgtacttg atggtaaaaa tagaatcttg taaaccgata cgttcgacga
    19441 tccctttcgc aactacggtt tcttctggca tttgatataa ttgccatttc aaacttgaac
    19501 tcccagcatt gattgcaatt gttttagaca tatgactctc cttctcgttg aatgattaca
    19561 gagcagatga tttccagttt ttaaactcgt tgaaaaattc cgtcaccttc tctggttctt
    19621 ttaatgaagc aagtttcaca agcaagactt cttttacttg ttgtgcttgt ggtccttttt
    19681 tctgtaagat caaaatactt ttttgcgatt gtttattacg gaacaattca tctggcaatt
    19741 gaatcattcc ttgaagataa ccttcttctt taaaccattt tttgatttct tcactttgat
    19801 ccgtttctaa aaatccactc ggcatcaaaa acaaaccaaa accatccggc ttcacatatt
    19861 tcattgattg ctctagcaat aaatgatgtg cataactatg gccttcttcg gtacttgtta
    19921 aaaactcttt tgctttttcg tcattcggat aataaccgat tggcaaatcg ctgattgcaa
    19981 aatcgacagg atctattaat aattcctgca acccgtcttg atgaaagtat tgaacatttg
    20041 cttgtgtcaa atcacttgta gaagctgcga cagccaacaa tgtatcatca atatccacac
    20101 caattccttc tgtttggtac cccgcattcg ataggtttaa aagaactgtt aataacaagt
    20161 tgcccattcc tgccgcaata tccagaatct taaccggtgc ttttttattt gtgtatagtt
    20221 gttcaataag aaaaacaaat aaaaaaccga tactatcagg agtgagttga tggttcgcct
    20281 gcaaatgttc tgtctggctg cccttcaata aaagtaattg agacagtctg cgccattctt
    20341 ctggctcgaa agaaagtttc ttcaactctt cataaagagc tgtgatccgt tgcgttgtct
    20401 cttttgtcgg cactccatcc accacacgta cttgataatc atctattaaa ttttctgcat
    20461 tttcgacata ggcttctaaa aaggaagttc ccaatgcatt ctgtaaaagc tgaatagctt
    20521 ccaggttttg attaaaccct tgttccattt tttctggtaa catggtgcac ctcatttcta
    20581 gatatattat aacaattttt tcacctgttt aattctatcg aacaattgac aaaatttcaa
    20641 tcgttttcac tgttttttat ttgtgaaacg gcaaacattt ttttattaaa aacagtgtta
    20701 taacagaaaa gagggcgttt tcacgaacat tccatcattc tgtactagta tctgtatcat
    20761 tttttacttc ttctgtgaac cgaaagataa acggtgagac ttccacctct aagaataggg
    20821 actgttgtac ccactgatat gttacagatc cagttgtgta gtaaaaaact ccttccgaac
    20881 tttcggcaag cgtagactgt tccgataaag ccatctgctt cattattttt gctatataat
    20941 aatttttgat tcgtatatta agatccattg acaaacggta gctttctaat gctgccatca
    21001 ataaaaacgt acataaaaga aataaaagca agacagaaaa taaaatagag cccttataac
    21061 attttctact gatcgttttt tgtccatatg gcatatccat cttctccatt ttcaaagtgt
    21121 acataaaaag aaattgtgtg ttctttttcc aataattgaa gctcagtgat acttgttaac
    21181 ataggttgat gtccgccgga agttcttctt ttacgtatca ttgcctgata tctttcgatg
    21241 atatactgtt tatcctcgat cgtgtagtgc agttgctgat ttttcacaga gacattgtga
    21301 ctgctcttga gttcttcttc taattgtgct aaaaatatta gccactcttt ttgatcttta
    21361 cgctgcaaaa attcacttac ttggatggtt tgctgaatca aaagattgaa tgtgagcaat
    21421 agactgctca atatcatcag tccaattaga cattcaatca gcgtaaatgc tggaattact
    21481 tgttttttca atactgatcg cttctttctc catattgaca aacacttttt cgattactcc
    21541 attattatcg acaatttgga tatcgtatgg gatcatttct tcccttcgct ctggtctttt
    21601 gtgatacatc cgctgcgtct tgatatcttc ataaatcatt cgaataaata gcagttcctc
    21661 cccacttttc ttttctgcct gaagcaaaaa cagctggcca aagctcacta agtagacaca
    21721 gacagaagcg atcataagcg ctgtaaggga ctccaacaaa acaaatcctg ttttattcaa
    21781 ttctttttgt atagcgacca ctccctatct ggaattgata cgtaatcgtc cgctcttttt
    21841 cttcccaata aaattgataa gcttttagtg aactttcatt cccggttcta gcagcaaaag
    21901 taatagaagc atggcgtttc aaggtaatct catcaggaat atctaatact tcccaattta
    21961 ttttatctgg attcggaaca tgaaaaatga tttgattctc ttcactattc caataaaagc
    22021 ctgtttggat ctgattcaca atagcgattt tttgcgtaga atagattcgt ttttcaaatt
    22081 ggccgaaaaa ttgatagact gccaattcat tcttccatgc agaaaatgcg agaactggaa
    22141 aagaaaggat cgttgtaagt aataaaatca gtaagagtga ttcaaacaac gtatatcctg
    22201 atgctaaaac cttaccttca aggccgctga tagatttctg catgttctcg tttgatatag
    22261 ccttcattca acaattcctc cacagtagga actcttccat tattttgaag cgcatagagc
    22321 tctttttgtg tgtctaccaa ctggatgatc gcttcacgac tttcttggtc tacatgattc
    22381 ctataacgcg acaaattagg gacgaacaac aacactaata cactgatgat cagtaagacg
    22441 attagcattt cgatcaatgt aaaaccgcta taataagata tttttctctt tttcatggca
    22501 aaacctcctc tatgcttccg taaacaggta ataaaacagc ggcataaact aataaaatca
    22561 aagccgctac gcccaaaaaa atgattggct gaataaaatg catccagtga tgaagacgtt
    22621 tgaaaagctg ttttcttgtc cattcgctgt aaaaaatcag ttctttacct aaacttccct
    22681 ttgcctctcc ctgtaaaaca attttactga attcttctga tagaaaacga taattctgga
    22741 actgctcagc caatgacata cctgcctcca atccttgaat caacttagag gcaagatgct
    22801 gaatcagact ttcttgtttc gtttccttta agcagactat gatttgcctt aattcgaaac
    22861 cttcatgaaa aagctttcca atctctagtg acaaataagc cgattgatac aaggaatata
    22921 tcggtccaat caataattgt ctgcttatcc attcactctg atcaacagga gacaacttca
    22981 tcagacgata ccttactagt aaagccaata gagtaaccgc taggatgaat agtagaacat
    23041 accaatgaaa gacttgtatc agctgaatac cccaatgctc ttgcatgacc attcctgaac
    23101 caataagctg aggcaaaacg aacaaccgca tactaaacaa gatacctagc aaaaaggtga
    23161 gcaatagcat cggatatgca agcagctttt tcaaatcatt gcgaaatcct tcaacaagcc
    23221 gaaactgttt ggcaatttcc aacaaagttg tcggcagatt cccatgacgt tcagctaact
    23281 cgatctgtac cagttgctct gctccatatc ctatttgcga aagtacctca ctaaacgctt
    23341 gcccttcctt tagctgatgt tggatatttg ccaaaataac aggtggaaac gaacggatcg
    23401 tgagtaataa ataaagggat tcttgcagac taaaaccaga taaaagcagt tctcccatgg
    23461 tttgtgcaaa ctggttttgc tggcttcttg ttaatccttt agaatagttc tgactgccaa
    23521 atattttcct gagcttcttc ataactttcc tcccatgatt tttttactga ttcttcctcc
    23581 ataaattgat aactccataa aacagaagga tggtgattgg catcttccaa aagttcttga
    23641 taaatgacac cagctaaaca ttcagatagc tcttcttttt tctttgtcag ttcatggatt
    23701 ctgaccaatg taccttcaag attgcgtgca tgtacggttg caaaaacccg gtgccctgtt
    23761 agtgctgctc ttaccgcagc atgtgcagtt gattcatctc ttatctcgcc aatgattagc
    23821 aagtctggac gatgccgtaa tgcgagtttc aaaagtgtat cataagtctg tgctattttc
    23881 ggattgatct gtagctgcag aaaaaacggt tcttctatct ctacaggatc ttcgatagta
    23941 atcacttgca atttttcttc caaagccaac cgatacatta gagaagtctt gcctgatccg
    24001 actgtcccgc tgaaaaggta taatccacgt ctgacggttt tctgtaaaat agttgcaaaa
    24061 tctcgagaac taaagcaacg atggggtgtt tcaaaagtac cgtataatag ccgaatcact
    24121 aaactctctc gttgaaggta atctcctaca gaagacaacc gcagacgaac ttcctgtttt
    24181 tctaaagaat aagtaaatgc acctaactga gcttttcttt tttctccgat atccatctgt
    24241 cccaaatact taaatcttga gatcagttgc tgtccatctg ccgaagagac tttcttccag
    24301 aatgttcgtt tttttccata gcgaaaatag atgtaaaata cttctgaagc tggcaatata
    24361 tatacgtctt gggcatgttt ggcaaaactt tcacttatca tttgatccga taatcgtttt
    24421 atttccatta ttctcccctc cacaaaaaac atacgtcttt ttgatctttt tgtttcaaat
    24481 aaaatgaatt tacaaaaaaa tattggcaaa agccgaattt atgttatact tataaaaatt
    24541 tatcttggga ggcactccat tggaaacaac attaaggaaa tatatcgaag tagcaagtaa
    24601 aaaaaagcct gctgacctag tgatcaagca tgctaagatc gtcaacgtct ttacgaaaga
    24661 aatcatggaa aaagatgtag caatctgcga aggaatgatc gtaggaatcg gtgaatacga
    24721 aggaaaaagc gtacataatg ctaatgggca atatctagtg ccaggattca tcgatggcca
    24781 tgtgcatatc gaaagctcac ttttatcacc aagcgaattt tcaaaagtat ccttgcttca
    24841 tggagttact actgtagtga cagatcccca tgaaatagga aacattgctg ggagtacagg
    24901 tcttgagttt atgatagaag atgcaagaca aacactcatg aatatctttt ttatgctacc
    24961 ttcttgtgta cctgtaaccc cttttgagac caatggtgca aatttaaatg caaaaaaatt
    25021 agcccctttt ttacaaaaac ccgaagtttt aggcttagct gaagtcatga actatcaagc
    25081 agtagcaaat aatgaaacag acatcctaga aaagatccaa ttgatgcacc agcataagaa
    25141 aaaaattgac gggcatgcag ctggtatcgg catggaagag ttaaatgttt atcctgcagc
    25201 aggtattcgg acggatcatg aagctacaac tgcaaaagaa gctaaagaac gtttggatct
    25261 tggtatgtat ttaatggttc gagaagggac cgttgcaaaa gatctggcat cgctactacc
    25321 ggcgatcaca cctgaaaatt caagaagatg cttctttgtc accgatgata aactgatcaa
    25381 tgatttagta aaagaaggat caatcgatca tatcgtccgt caagctattg cacttggaat
    25441 cgatccatta caagccattc agatggcaac attgaatgca gcagaatgtt ttgatttgaa
    25501 aacactaggt gcgattgccc ctgggtatca agctgatttc ttcttaacag acgatcttac
    25561 caagctgcca attcatgaag ttttctataa gggaacatta gttgttcaat ctgggcaact
    25621 ccaagaccat ttatttgctg atcattcaaa ttcatatacg tgttgtctgc caaaactaaa
    25681 cgctcaacca ttaaatccac attcgttaga gctgcttctt tcttcttcac aagcccatgt
    25741 gatcgaaatt atcccgaata gtctagtgac taaagatttg gtagaaaaag tggaccgaca
    25801 aggcgaacga ttcattcctt caatcgaaaa agaccaatta aagattgcgg tgatcgagcg
    25861 tcaccatcaa acgggaaaca ttggattagg catagtgaaa ggatttcagc tgaaacaagg
    25921 agcgatagcg acaacgattg cccatgactc gcataatcta gttgtcgttg ggacaaacga
    25981 cgaggatatg ctgtatgctg ctaatctttt gattcaaaaa ggtggcggga tgaccgttgt
    26041 caacaaacag caggaaatag cttgtgtttc tcttcctata ggcggaatca tgacacagga
    26101 acctttttcg atcgtctatg aacagttgaa tgaattaata actaaagcac atcaactagg
    26161 agcagctaaa acctttgatc cttttttgac actttctttc ttaacattat ctgtcatacc
    26221 cgaattgaag atcaccgaca aaggattatt ttcattttct cgcttccaat tgattccacc
    26281 ttgtggaaaa accgatgaaa taaatacatt ttaaatcaaa taaatcccag aaaatacatt
    26341 acatctgtaa tgttcatttt ctgagattta tttatggata ttaatgtttt tatgaaagca
    26401 gaaagttagt ttcacagact tttctttttg gtcttatctt gaaattgagc ttctactcga
    26461 taaatacgtt gaccttttga agaaaatttt tcttcatact ccgtcataat attgccttca
    26521 aattgacttt gatgtaaatc taaccatacc tgtttgagga tcatgccata ttgagaaaaa
    26581 cttgccagag aatactcaaa caatccttga ttatctgttt tgaaatggat ttctccattt
    26641 ggacgaagaa tctgttcatc tacagcaaga aaagtcttat atgtcaatcg ccgtttttca
    26701 tgacgttttt ttggccatgg atcggaaaaa ttcagataga tttgatctac ttcgctgtca
    26761 gcgaaatact ctgtcaacgc tgatccatcc acatgtagaa gtttcaggtt aggaagcggt
    26821 tgttcgatca acttgtctag tgcaatcgaa acaacactga cctgcatttc tactccaata
    26881 taattgattt caggatgggc ttttgccatt ccagtgatga actggccttt ccccatacca
    26941 atctcaatgt ggattggatg atcgttttca aacaattcat tccattttcc cttccacaat
    27001 gttggatcac taattacaaa attcgggtga gccgctaaca tttccgctgc tcccggacga
    27061 tttcggacac gcattattta tctccattcc tttcctctaa ttaaaaaggt ctggccaaat
    27121 ctccgcagag tttgggccaa acctcagcca ctgaaaattc gttttaattg taaaatttct
    27181 tcgttcatcc gtcgatgatc ttcacgtttg caatatctgg tgatttcttg gagcaaattc
    27241 atggccgcat accaatgaaa tttttcaagc atttcgtcag tcgggcgtat gccataactg
    27301 atcagccact gattccagct agacagtggc acataatttc ctaaaatcgt tgcaatatcc
    27361 accgcagggt ctgcaaacat gattgaatcc caatcgacta gatataaata atttttacat
    27421 actaaccaat tacgatggtt tacatctccg tgtactactg ataagttttc cttatctatt
    27481 gacgggatct gctcacgaag aaaacgatgg acaatttgca gaaattgatt tttctggagt
    27541 tcttccggta attctttttc atatttttgc agcatcttct ccggcgtcat gacacgacca
    27601 ccgatacggg tcagcatggt tttaagagaa tttgaatgat gtaaatggta caaaacatca
    27661 atcacgtcgt ttctttgacc aatctcatta ggagtcagca agcgaccttc cagccactct
    27721 tgtgctgtca aagtatcgcc attccccgtt cgttttgtcc aaacaagttt tggtgtaatt
    27781 ccttcccttg aaagagcagc caacatcgga gtcgtgtttc gtttaataaa gactttgtcc
    27841 tcatcacgca tgcctatgta tgtttttcct gtgtcgcctt tgatgggacg taaccgccac
    27901 tcttggtcta actgaaactc catcatgcat tctcccgtcc tcttactctt tgttttgcag
    27961 tacttattat tctagtcgcc tgaacactac acgtcaagca catatacgtt ctttttcatt
    28021 taattgccat tcaaaaaacc agcttttctc aaaaaaaata gaacgaacag cattcttact
    28081 gtcaacgctc tatttttcat tctcattttt accctacaca gctttgtttt cagtctgaca
    28141 tcttcttcaa acgagttggt acatatagtt taacaaagag catagtaaca atcaaataag
    28201 ccgctagcgg aacccatcgc tgctgcccat ctaatacgac tgcagcaatc aaaccaaaaa
    28261 taaccgacgc gataattaat acccatgtga gtagatactg aatcgcctgc ttcttctgat
    28321 tcttatggat tggataaagc tgaacgagaa ccatatagcg gaactggttc gctaaaggta
    28381 acagttggaa accgatcaaa tataaaaata agaaaccgat aatcagacta aaataaaagt
    28441 cgactactcc ggcaatcaat aacccaccta taattactaa tcggaaataa agaccactaa
    28501 aatcagaacc tcttgccaat cttcttgcaa acaagtaaag ataagtgttt tttgattctc
    28561 ttttaatatg agtcaatata ggatccaaat agcgtcttct tttgatcttt gccgtaattt
    28621 caggaacatc tgtgaataaa ttgataaatt gatagatctg atgcaaccta tgttcttcta
    28681 catgcaccat tttctcccaa tccaatcctt tttcttgttg cttccacaaa aaccagtaat
    28741 atagacttgc ctgtactaat gcacctgcta ttcctgccca tattagccaa taaaatgata
    28801 gaaacaaaaa gatcagagaa gaaaaaaacc agcctaattt ccaataacga ataacttgtt
    28861 gatctagtcg aaacaattct gctctcttga tttgcagatg actgaatttc aaactccata
    28921 atatgatcag atagacgaaa aacatcgaaa aggattgctc cgtactaacc accactaatg
    28981 gcatggccaa tccgcatgcc aatatcaaaa agatcaacgg aaaaatgcaa gaatagcgaa
    29041 aagctcgttc caaataggtt tgcatttctt tttctttagg aagtaaaaaa acaatatctg
    29101 ctgcctctgc aaaagtagcc atcttaccca cgaataaaca gagcatccaa aatacagaga
    29161 ttatgagccc tccaattgca aacggtggct gcagggtttt caaccaatta gaataatata
    29221 atcccacacc gccaatcaag aaaagacaaa ctaaaacaaa atgatcgttc aatacataac
    29281 gcatatattt catcattttc ttttgatgtc ttgctaaacg tttttgaaaa aaactactca
    29341 tgtgcctgct cctttgttaa agcaatatag atttcgtcca aagaagaatc cggcagctga
    29401 aattcttttc tcaacatctc aagagaaccg tctgctcgta tctctccgtt atgtaagacc
    29461 acaaaacgat cacaatattt ttctgcagta gctaaaatat gcgtagacat caaaataccg
    29521 gctccttctt ttttcatttc taccatcaat tcaagcaatg cattgatagc tagtggatcc
    29581 aagcctaaaa atggttcgtc gatgatatat aaagaaggct cgatcaaaaa ggcgcataag
    29641 atcatcactt tctgtttcat tccctttgaa aaattagctg ggaaccagtc aagacgatta
    29701 gataaccgaa aagtctccaa caacttttct gctcttttca ttgctatatc cataggtata
    29761 tcgtaggcca tcgcagtgac ttcgatatgt tctctcaatg tcaattcttc atataaagaa
    29821 ggagtctctg gaatataccc aatttgtttg cgatattctt ccgtattttt ctttaaggat
    29881 agatgattga tttctatcgt tccttttgaa gcttccagca gaccgataat gtgcttgatg
    29941 gtcgtacttt tccctgctcc gttcagtcca atcaacccta ctatctctcc aggctgtaca
    30001 tcgaaactga tatcttttaa cacagggaga tgtccgtacc cccctgtgac atgatcgata
    30061 attaaactca ttctttttcc tccgccattt atttttattt attataccac gaagaaaatg
    30121 acggtatttt gtttttttcc ttacaatagc tttaacttat ggtaaagtta agctgaagta
    30181 tttattcttt atagaaagga tgttttatat ggaaaactgt attttttgca aaatcatcaa
    30241 tcaagaaatc ccaagctata aaatatatga agacgacaag gtctatgctt ttttagacat
    30301 ctctcaagca acgaaaggac acacattagt tgtgcctaaa cgtcatgtag cagatatttt
    30361 tgaatatgat cccgaacttg caggagaggt tttttcgcgt gtgcctaaga ttgctcaagc
    30421 attggaaaaa gcttttccag aaatgaaagg tcttaatatc ttgaataaca accgtgaact
    30481 agcctatcaa tctgtttttc attcacatat ccatttagtc cctcgctaca gcaaagagga
    30541 tgatttttct attcactttg tcaatcatca agatagttat ggatcggaag aactaaaagc
    30601 tattcaagaa acaatcgtaa agcaggtgtc ctgcgatgat taaaaacttt ttcaaaggac
    30661 tgttatttgg agctgctgct ggcactgtcg gaggtctgtt attggcgccc aggagcggga
    30721 atgaaacaag aaagaaactg atcgaagaat tggaagaagc aacaactctg acaaatgatt
    30781 taaataacag cctgaatcat tttaaaagcg cattgatcga aacaaaagaa acagctgaat
    30841 ctgtgattcc tgctttccaa gaagcaatca aaaaagatat cgaagatttt aaattccaag
    30901 ccgaaccacg tatcacacaa atcaatgaac aagtagaaaa actaaaagct gatctgccag
    30961 agtatcctga acaaatataa taatgattat tttctcaaca gatttaacta tataagtgag
    31021 aaaagtttgc gtatttccta ggaatgtgat gaaattttga attttgtttg aaactaccac
    31081 gacaacgttt ttaaaggaaa tgggatatgg tatagttaat atataaaaat aataactaat
    31141 tacaggagtg caaattttaa tgaaaaaaaa atcaattatt ttggcagcta ctagtgcgtt
    31201 ggctgtttta actctagcag cttgttcagg cgatactaat aaagatatcg caacgatgaa
    31261 aggcggaacg atcactgtat ctgatttcta tgatgaagca aaacttgaat catcgaacca
    31321 atccttagta caacggatga tcatttataa agtattcaat aacaaatatg gcgataaagt
    31381 aaccgataag caagtggatg cagaatacga caaacaagcg aaatcattag gtgatacttt
    31441 tgaatcacaa ttagaagctg ctggatatac gaaagatact tataaagaat atattcgtaa
    31501 caatttagct tttgaagctg gcttgaaagc tcacgtagat attacagatg acgatttgaa
    31561 aactgcttgg aaatctttcc atccagaagt agaagcacaa atcatcaaat tatcaagtga
    31621 agatgaagca aaagatgtga aaaaatcagc ggatgatgga gatgatttct ctaaattagc
    31681 caaagacaaa tcaactgata cagagacaaa agaagatggc ggtaaagtta aattcgactc
    31741 aacgacaaca actattcctg cagaagtaaa agaagcagct ttcaaattga aagacggtga
    31801 gatctccgat gttattacta caacaaatcc tacttcttat gcgacagaat actacgttgt
    31861 caaaatggtg aaaaaccaaa ataaaggcaa tgacatggat aaatacaaag atcaactaaa
    31921 agatatcgct actgaaacta aactaagtga caatgcattc acaactaaag taatcggtga
    31981 agaattaaaa gatgcaaatg taaaaatcaa agatgatgcc tttgaaaatg tgttgagtgc
    32041 attcacaaca acttcatcaa gtacgaaaga ctcaagtgaa actacagctt caacaaagag
    32101 ttctgacaca aaatcaactg attcaacaaa agaatcttca actgaagaaa caactgattc
    32161 atcaaaataa aagaaaacaa aaaaagcgaa gacttatgtc tcgctttttt tgttttgaat
    32221 gaaaaaaata aatttttgct ataaatcttt tggtacatag aaacttcggt tgtccatacc
    32281 gaaaatccga tcggtgtatt ttcctggttc tgtttgacga atcgaagtca acatcatctg
    32341 catcgaagca tcgatattgt cgatttgatg taatatttct gcttccatta ttcttggacg
    32401 tacaggtgag ccatattcta ataagccatg gtgtgctaaa accatatgac gtaatacaag
    32461 aacatcttcg tcagcttcgc tgattttcaa cgcaagacag gctttagtta tttcttcgtc
    32521 aatcaatacc agatgaccga tcaaatttcc tgcaagcgta tattctgtcg acataggacc
    32581 ggaaagttca atcacctttc ctaaatcatg caaaatgata ccagcataca acaatgctcg
    32641 attcagctca ggatattcat cggcgattga tttccctaag tgaagcattg tcaaagtatg
    32701 ataagctaat ccactggcaa atgcatgatg gtttcttttt gctgccggaa actcaaaaaa
    32761 ttgtttttga tatttattaa gtagatagcg aacaatacga ttccaatgcg cattggtgat
    32821 ctcaaacaac aattgattga tttcttcttc catttcttct ttcttcaaag gggctctttc
    32881 catgtataaa ctaggctctc ctggctcatc tacccttgtt aagcgcatat gaagaatctt
    32941 tacttgaggg tttccttgat aaacctctct tttaccgttt agaaaaacga ctgtgccggc
    33001 tgtaaattta ttgatttcct cctccgaagc atcccagtat ttaccatcga tagtacctga
    33061 agtatcttgg aaagtaaaag caataaattt ttttccgttt ttagcgattc ttacatctgc
    33121 acttttgatc aggacaaatt gttcaaactg ctcatcgatt actaaatcac gaattttttt
    33181 cattatttgc tccttctaat tgttttacga tttggttcat ctcatggtaa tagcttacca
    33241 tttctttatc agaagaaaaa caaattattt gatacttttc tccaaattta gctaaaagtt
    33301 cagctaattg atactttcgc tgatgatcat aatgaagcca gccatcatca atgatgatcg
    33361 ggcatatcga acggttagct tctaatgaga ggtaagcaaa tctcatcgcc ataatcagct
    33421 gatctttcgt acctgtagat aactcataga tgggaaattc tgtatatccg tctgttaaag
    33481 tcagtgtatc atttttcagt aatatcttct gataatttcc ttttgttaaa atacctaaat
    33541 aatcacctgc tgctcttaac aactggggta attgtctttc actcatctct gttgaaagat
    33601 cacctaagaa tgtcccggcg atttcaagtg ctccccattc ctccagtaat tcctgtaatt
    33661 ttgctttttg ccggctagct tgttgataaa gttcgtccaa tgtgccgtct ctttgcaact
    33721 cttctacttg aagttggagt cgttgccgat tttcacttgc ttctttttct tcttgctgga
    33781 gttgggattg cttttctttc agttggcgca gtcttacaga taatttctct tttgtcagct
    33841 cttcaggaaa tagtggagta agcatttccg ataattcatt gtatcttttc atctgctgtt
    33901 cggcttgttt ggctttttgt aaaaagacag gtacttccga aggataagat aagcctgctt
    33961 gagtcaattg ttcgctgtat tcttgtatcc atactttttg tttctttttc aattgattga
    34021 tttcttgctt caataaagta ttttgctgat agcttctggc aaatttcatc tgttccatct
    34081 cttgtataaa acgggaaagc aataccattt tttctgaaat tgtcttatct tgtatcggta
    34141 accattcttt aacaaactca aatttttggt cgatttgtcg tagtttttct tgtatttcct
    34201 gttctctttt atgtttccgc tcaaatacgc catataattt ctgataatct atgacttcct
    34261 gtccatattc tttcatgagt ttgagcgtat ccatctttcc taagtgccga tgatccgcaa
    34321 aacgatatac ttcttgctga agaatctgtt tttcttctcc agttctcttt tgtttttctt
    34381 ctgcttcagc taattgagct aaggctagat caagttgtcc tagtttttct tgccacattc
    34441 cttttatctc agagaaatca tttttagttt taatttttgg ttgccttttg tagaagaaat
    34501 agccaatcat gcctgcacca attagtaaaa ggatcacttt caagggcatt ggtacaaata
    34561 tacctgcaat gattattgct ccgccagcca tcagcatata cggtttagat gaagagactg
    34621 gtgctatctc tgactgattt tgtaataatg ctggataggt ctcttccaag tcattcactt
    34681 ctgcttcaat catctcttgc tgttctttag ctatttggag acgcacagaa atctggcttt
    34741 ctagatcagc aatccttctt tcttcagcca gaagatgctt ccattcttct tcatataaaa
    34801 attcttccgg taacggattt ttctgccaat gccacctttg ttccaaaatc ttcaattctg
    34861 aatttgtttc tctagtctca atttgtaatc tttgccattc ttcgtttaat tgttccgctg
    34921 taaattgaag ttgctggaga tcttttagtt cctgctcctg ctctaaataa aaataatatc
    34981 tggctgactg ctgatccatc ccgctatgct tttccagtgt tgcttctaat tcttccaaac
    35041 gctcatttgt ttgctgatac cgtttggaga aagactccag atctaaaaga agttgctcat
    35101 caggaagacc tgtagtgctg ttttttttga gagattgcca ttcttcatat aaagaaaaat
    35161 taagttcttg ctgtctggct aatgaaaatt gtttgttcgc ctcttttaat tgtgccaata
    35221 actgctgctg ttgcgactcg tattcctgtt cttgttggat caacttgcca aaataagctt
    35281 cctgttcttg tttttgatga attttttctt gcagctcttt ccattcattt aatttttgat
    35341 tgattagtaa tttttggcct tttttcttga ataattgttg tgctttctga taatattctt
    35401 gccgcttttg gaatacttga gaactgcccg tgatgcctaa agaaatcagc gcatcatgta
    35461 gctctttttc tctcaaatga tctagttcca tcaactgctc ttgctggaaa gtaaatacct
    35521 gctggaataa ctctttcgtc aaaggtgcga tcagtttttc tagtaactca tcacttccca
    35581 cttgatcccc cagccacacc ttcgattttc cgcgattttg ctgcttgtag cgctcgacag
    35641 caaattcacc atatacagga tgtttaagcc atattttgcc gccataggct gagccgtctt
    35701 ttggtgtata atcctttttt ttacggcctt ttgaagggaa accaaataat attgcttgaa
    35761 taaattgata aatcgttgat tttcctgctt cgtttccccc atagatcagc tgattgcctg
    35821 aataaaagtc aaaacttttt tgacgccatt ttccaaaagc agcaatctcg atccttaaaa
    35881 tcttcattcc tgatcctcct caaataaaaa atcttgatta agcagctgct tggctttttc
    35941 aagtgtttct tcttgatatt ccggcagttc gctcaatatt ctagccgctt cttgatgact
    36001 atatacttca tttaaaattt gttggaaatc ttgaggtgtc tgatagtatt gaaataactg
    36061 ttccatcaat ttagcagaag cagcaagtgg ggtcttcgta taaaaagtat tctctatgag
    36121 agaaatacgc caaataaaga aatcatttat cctatctgag aattcctcta acaaatagtc
    36181 taatagctct cctgattgta tccgttccaa aacatctgta ttgagatggt cataatcttt
    36241 taattttatc tcaattaaac taaatccatc ctctactttt gaaagctgct gacgaatgtg
    36301 aacaatgacc tcttttgtcg ttcttgcttt cctcaatgat atttcctttg tcttccaata
    36361 gacctctgct actttaatcg gatgtatttg gcaacttcct ttacttaatt caactagtag
    36421 aatagatgta gaagcttgtt cctttttagt atgaccttga ggcgcacctg gataaacaat
    36481 tgtttgcttt tcatttaaga ccgttggcac atggatatgc cctaaagccc aataatcgta
    36541 tcccttttct tgcatctttg agggttggaa aggagcatag tttccttttt gagagacgcc
    36601 aggttccccg tggtacaacc caatatggta atctgtgagt tctctagaag gaaattccat
    36661 cactttgtct ttttgaatcc acggttgacg ataactaaat ccactgagag agacagtttc
    36721 tccacttttt atcgttattt tttttgtctc taccgtttcg gatgtaaata aatggacatt
    36781 tttaggaaag gagaaccaat accgctcttt ttgatagaaa tcatgatttc cgaatattat
    36841 aaatacaggt atatggttct tttctaaccg tttcatctgt tctgaaaaat gtttctgtat
    36901 ttttaatgaa ggacgatttt gatgaaaatt atctcctgca agtaaaacaa aatcaacgtt
    36961 atttattatt gcctgttcaa tgatatttga taaaacggtc agattcgtct tcaatagttt
    37021 ttcctgtaca gttttatcaa gagttgtcag tccttcaaat gaccgatcca tatgtaaatc
    37081 tgccgcatgg ataaatttca ccattaaaaa tttcctcctt tttttctttt atcataccat
    37141 tttcaatgtt ttttttcatc aataaccgaa aatttgttcg tattatgcaa agaaattacg
    37201 ataaatggcc gacttcaaaa aataagttta ttcgaaaaaa agattgtgac atgatttttg
    37261 tcacaatcct aaatatctat gttatattcg cttcgtttga cactgaagaa gtactatttg
    37321 tactggaatt tatgctccat ttttccgtat ttagaattct atgttctata ctaccttaat
    37381 tagtttatat ggtaagaatt ctttcaaatg cttgagatac tccgtcttta tcatttgaaa
    37441 cagtgacata atcagctttt ttcatgattt cttgatgact atttcccatt gcaaaactaa
    37501 tatctgcact ttcaaacata gataagtcat ttaaactatc tccgatcgcg attctcaaag
    37561 gtttctttaa attgtagatt gattccaaac gatcaaacac cacaccttta gatacatttt
    37621 gaggtacgat ttcaatattt acaggtgccg attcagtaac agacacattt agcatctttt
    37681 tcaaatggtt aaatatatta gctttcaatt gacgattagg aataaaaata aaaaatttgt
    37741 aaatgctgat agcatccttt tctataattt cctcaatatt attataaggg attgaacaaa
    37801 tagatttttc atactcaagc tcatagttta gctctttgat agaaaattca ctccctacct
    37861 cgtgaaaaat ttttttcatt ttatttttat aattctgaga ttcataactt ccgagatttg
    37921 tatatatttt ggttggaaat ttcgatagga cagaatcaat tttttttaca tcatttttat
    37981 cgaaggaaga ctcaaatatc tttttttctc catcaaatcc aacagaacca tttaacccta
    38041 ctgctggtat ccttaaacca gattttttga gaatattatt aatgtcttct acttctcttc
    38101 cagaacaaat gaatggaaaa cagtcttccg gtaaatgttt cattgttctt gcattttttt
    38161 gactaatgct ctggtcagaa ttcaaaagag taccgtctaa atctatacca attagtttca
    38221 tttcatccat gtctcctata cgttgattat aaattcactg cttattgtaa gtctaaattt
    38281 ctcaca
//
LOCUS       DADOOS010000011        37226 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.011, whole genome shotgun sequence.
ACCESSION   DADOOS010000011 DADOOS010000000
VERSION     DADOOS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.011"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            30..803
                     /locus_tag="J8C37_000488"
     CDS             30..803
                     /locus_tag="J8C37_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303949.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(884..1453)
                     /gene="thiT"
                     /locus_tag="J8C37_000489"
     CDS             complement(884..1453)
                     /gene="thiT"
                     /locus_tag="J8C37_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAZ5303950.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1519..1621)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1627..2598)
                     /locus_tag="J8C37_000490"
     CDS             complement(1627..2598)
                     /locus_tag="J8C37_000490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAZ5303951.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2692..4065)
                     /gene="glmU"
                     /locus_tag="J8C37_000491"
     CDS             complement(2692..4065)
                     /gene="glmU"
                     /locus_tag="J8C37_000491"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAZ5303952.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4568..5386)
                     /gene="purR"
                     /locus_tag="J8C37_000492"
     CDS             complement(4568..5386)
                     /gene="purR"
                     /locus_tag="J8C37_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAZ5303953.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5553..6377)
                     /locus_tag="J8C37_000493"
     CDS             complement(5553..6377)
                     /locus_tag="J8C37_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAZ5303954.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(6350..7030)
                     /locus_tag="J8C37_000494"
     CDS             complement(6350..7030)
                     /locus_tag="J8C37_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAZ5303955.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(7053..7991)
                     /locus_tag="J8C37_000495"
     CDS             complement(7053..7991)
                     /locus_tag="J8C37_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAZ5303956.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(8236..9087)
                     /locus_tag="J8C37_000496"
     CDS             complement(8236..9087)
                     /locus_tag="J8C37_000496"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAZ5303957.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(9241..11814)
                     /gene="mprF"
                     /locus_tag="J8C37_000497"
     CDS             complement(9241..11814)
                     /gene="mprF"
                     /locus_tag="J8C37_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAZ5303958.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11955..12761
                     /gene="proC"
                     /locus_tag="J8C37_000498"
     CDS             11955..12761
                     /gene="proC"
                     /locus_tag="J8C37_000498"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAZ5303959.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12840..14396)
                     /gene="rny"
                     /locus_tag="J8C37_000499"
     CDS             complement(12840..14396)
                     /gene="rny"
                     /locus_tag="J8C37_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAZ5303960.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14807..15856)
                     /gene="recA"
                     /locus_tag="J8C37_000500"
     CDS             complement(14807..15856)
                     /gene="recA"
                     /locus_tag="J8C37_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAZ5303961.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15965..17206)
                     /locus_tag="J8C37_000501"
     CDS             complement(15965..17206)
                     /locus_tag="J8C37_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAZ5303962.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(17446..18024)
                     /gene="pgsA"
                     /locus_tag="J8C37_000502"
     CDS             complement(17446..18024)
                     /gene="pgsA"
                     /locus_tag="J8C37_000502"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAZ5303963.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(18233..19117)
                     /locus_tag="J8C37_000503"
     CDS             complement(18233..19117)
                     /locus_tag="J8C37_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5303964.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(19895..21181)
                     /locus_tag="J8C37_000504"
     CDS             complement(19895..21181)
                     /locus_tag="J8C37_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAZ5303965.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(21174..22442)
                     /locus_tag="J8C37_000505"
     CDS             complement(21174..22442)
                     /locus_tag="J8C37_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAZ5303966.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22694..23155
                     /gene="mscL"
                     /locus_tag="J8C37_000506"
     CDS             22694..23155
                     /gene="mscL"
                     /locus_tag="J8C37_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAZ5303967.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(23215..23661)
                     /locus_tag="J8C37_000507"
     CDS             complement(23215..23661)
                     /locus_tag="J8C37_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5303968.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23754..23999)
                     /locus_tag="J8C37_000508"
     CDS             complement(23754..23999)
                     /locus_tag="J8C37_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303969.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(24114..24692)
                     /locus_tag="J8C37_000509"
     CDS             complement(24114..24692)
                     /locus_tag="J8C37_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ5303970.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24769..25299)
                     /locus_tag="J8C37_000510"
     CDS             complement(24769..25299)
                     /locus_tag="J8C37_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAZ5303971.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(25303..26163)
                     /gene="rlmB"
                     /locus_tag="J8C37_000511"
     CDS             complement(25303..26163)
                     /gene="rlmB"
                     /locus_tag="J8C37_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAZ5303972.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(26156..26551)
                     /locus_tag="J8C37_000512"
     CDS             complement(26156..26551)
                     /locus_tag="J8C37_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAZ5303973.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(26548..27960)
                     /locus_tag="J8C37_000513"
     CDS             complement(26548..27960)
                     /locus_tag="J8C37_000513"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAZ5303974.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27957..28493)
                     /gene="cysE"
                     /locus_tag="J8C37_000514"
     CDS             complement(27957..28493)
                     /gene="cysE"
                     /locus_tag="J8C37_000514"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAZ5303975.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(28815..30275)
                     /locus_tag="J8C37_000515"
     CDS             complement(28815..30275)
                     /locus_tag="J8C37_000515"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAZ5303976.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(30445..31602)
                     /locus_tag="J8C37_000516"
     CDS             complement(30445..31602)
                     /locus_tag="J8C37_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAZ5303977.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(31636..33006)
                     /gene="radA"
                     /locus_tag="J8C37_000517"
     CDS             complement(31636..33006)
                     /gene="radA"
                     /locus_tag="J8C37_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAZ5303978.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(33069..33596)
                     /locus_tag="J8C37_000518"
     CDS             complement(33069..33596)
                     /locus_tag="J8C37_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAZ5303979.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33826..34632
                     /gene="proB"
                     /locus_tag="J8C37_000519"
     CDS             33826..34632
                     /gene="proB"
                     /locus_tag="J8C37_000519"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAZ5303980.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            34629..35876
                     /locus_tag="J8C37_000520"
     CDS             34629..35876
                     /locus_tag="J8C37_000520"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAZ5303981.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35952..36296
                     /locus_tag="J8C37_000521"
     CDS             35952..36296
                     /locus_tag="J8C37_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303982.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(36413..36739)
                     /locus_tag="J8C37_000522"
     CDS             complement(36413..36739)
                     /locus_tag="J8C37_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303983.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT        10404 a         8081 c         6644 g        12097 t
ORIGIN      
        1 gattaagagg aaataagagg aggaacatga tgactgagtc agtttatgta catattgata
       61 ccacaagtaa tgctgtttta acaaaagggc tgactgctac tgactttgca aacagtatcg
      121 tgcattttcc tcaaaacttg ctcttgcttg atccatctgc ttctgctgga gaatatgaat
      181 cccatacagg gctgaaagta atccgtggta cagaaaatat tcagcgtttc ttttcctctt
      241 ctcgtaacag gcgtggattc gatgaattga agtggatcga ctttactgat ttaacgatgc
      301 tgaaagaatt gacaccttta gaaatttcag aattattata ttttggtcat atgaaaacac
      361 atcttcattc gccgtttttt tataaactac agaataattt tgtatacttc gatcttaatg
      421 atcagctgaa tcggatttat tatcgctatt tagatgaatt ttataggatt tttgctagta
      481 aattaagttc gatcttgtca gataagatca atgtaaaaaa atcttttttc aaaaaagaaa
      541 cacctgttga aaaaatcagt gtggaattat tgaaagaaat gcgtaatttg atgcaggaag
      601 ggatcatttt ttcttttgat caagttggtt tgatcgatgg taaatatcgt atcccaatct
      661 atgtgattga agattatatt tggaaattaa aaggcacccg gtatcgagaa gaggaaatca
      721 tcgctacttt aatctactct gtcaccgatc gacactggca attggtagaa gatgccgatc
      781 tttcttatct gccattgaag tagatctatt tcttatcaaa aaataaaaag gaaacaagaa
      841 actaggacat gattctgtct caagtttctt gcttcttttt tatttacagc tcctgtcgtt
      901 tatctggcac aaaaatcgaa ggagtcgttt gttggatccc taataaaacg atgatcgtta
      961 caatagctgt actcaacccg ctggcaccat tcattacgaa agaaaagagc caaggattca
     1021 tcccccatag tgcatacgct ccccagaaaa ccactcctgc aataaaatgc caaaaatatc
     1081 tggaaagtgc gccaatcaat gcaccaaaca cgatgctttt tctaatttgt ttcgtattat
     1141 cgtctacata gcctttttgg acatttgaag ccatgagccc tgctgcgcct gcaaaagtat
     1201 aggcaatcgg gtactcaatc aacacttgaa cgacacttaa ataataaact tgtcccaacg
     1261 gaaaatgtag gatgccccaa atgaaacctg ccagcatccc tgcctttgtc cctcttctta
     1321 acgcaaaaag agtcaatgga atctgcccta atgaaataga aaaactgctg ccgatctgga
     1381 tcggaatcaa cgacaacacc atcgctaacg ctgcaacaat cgttccttct acccaaactc
     1441 tattttttcc cataaaaaaa cttcctcctt tgaataaaaa agtcacaaag gaggaagcag
     1501 ctttcgaaaa tagtcaaaca gctgtttatt cacaattcct acgctcgcat aaccgataca
     1561 ggtacgaagg gtttagaaat agattcaatc tcagcccaat tggactcccc tttgtgagtt
     1621 tatttattat ttattttttg tttcaaacaa tggactaact ggtttgtttt catggatacg
     1681 tttgattgca tctcccatta gtccgccaac acttacttca tcgattttgt cgattttacg
     1741 atcttctgga agatagatcg agtctgtaac aactagacgc tcgatagctg aatcttctat
     1801 tctttgtaaa gcagggccgg ataatactgg atgtgtacat gaagcataca cactagttgc
     1861 accagcttct ttcaaagcat tggcagccaa tgtgatcgta cctgctgtat cgatcatatc
     1921 atcgatcagt acacaagttt tgcctttgac ttgtccaatg atattcatta cttctgcaac
     1981 atttgctttt ggacggcgtt tgtctatgat cgcaatcgga gatttcaaaa attccgctaa
     2041 tttacgtgcc cgtgtgacac caccatggtc tggtgaaaca acgacaacat catcgccttt
     2101 gattcctttt tctaagaaat aattggcaat aagtggcgca cccatcaaat gatcgactgg
     2161 aatatcaaag aaaccttgga tttggactgc atgaaggtcc aaagtcagca ttcttgttgc
     2221 accagctttt tgaatcatgt ttgctactaa tttcgctgtg attggttccc gtgcacgtgc
     2281 tttacggtct tgacgcgcat agccataata aggcatgact acgttgattg tttttgcact
     2341 tgctcgtttc agtgcgtcaa tcatgatcag caactccatc aagttatcat tgactgggct
     2401 gctcgttgat tgaataacat agacatgcga tccacgaata ctttcttcaa tgtttacttg
     2461 aatttctcca tcactaaatt gagtaacaga tgattttcct aactcgacgc cgactgctgc
     2521 tgcaattttc tctgctaatg ggcgattgga atttaacgca aaaattttta atcttgggtc
     2581 aaagtaatgt tttgacatgg ggacctccac tttctttttc acaactctag aattctacct
     2641 aataaatagt agtcatattt tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag
     2701 ataagggagt ttttttgcat atccttcttt attgacttgt cttgctctcg caattgccag
     2761 cgcatattct ggaatattgt ctgtaattgt tgacccggca gcaatcgaac tgtttttagc
     2821 tacctcgaca ggtccgatga tattcgtgct agaaccaata aagctgtggt cgccgacagt
     2881 cgtatgatgt ttgttctttc catcatagtt gacgaagaca acaccacatc cgacattgat
     2941 ttcttctccc agcgtagcat caccaacata agtcaagtga ccgactttag ttcctttgcc
     3001 gatttgagca tttttcactt ccacaaagtt tccgatatgg acgttttcgc caatttcagc
     3061 ttttggtcgt aaatgtgcat aaggtcccac atctgcatgt ttcttgacat ggctgctttc
     3121 aatgactgag ttttcaatga cgacgtgatc ctcaatccta ctgtctacga tttttgaatg
     3181 tgacccgatg acacaatcag aaccaatgac tgtatttcct tggatttgta cacctgcttc
     3241 gatcaatgtg tctggaccga tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac
     3301 aaagcttacg ccattgacca tatgcatctg gttaattcgc tttctcatga tctcatttgc
     3361 tttagctaac gcgatccgat cattgactcc cattgattct tcaaaatcat ccgtttgata
     3421 agcagcaact gtatggcctt cttcttttaa gatttcgatg atatctgtta gatagtattc
     3481 accttgagca ttgtttgttc caactttgct taacgcatcg aacaaagctt cgttgtcaaa
     3541 acagtaagtt cctgtattga tttcttgtac aagagcttct tctggtgttg catctttttg
     3601 ttccacgatt ttttctacga tcccaatatg gtcacggatg attcgtccat atcctgttgg
     3661 gttttctgct tgtgccgtca agatcgttgc acttgcattt tttccttggt gatattcaaa
     3721 aagattgttc agcgtttcag tagtcaataa tggtgtatcg ccactgataa ctaacgttgt
     3781 tccttttttt ccttccaaga aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg
     3841 ttctgcttgt aacgcatatt tactgcgttc tcctagttgt gctttaactt gttctgctcc
     3901 gtgtccaacg atcgtaatca cttcatctgg tttcgtttca ctgacccgat taatgatatg
     3961 ttctaccata ggtttaccag aaacgggatg taaaacttta tataattttg acttcatgcg
     4021 ggtacctttc cctgccgcca aaataattgc ataacgtgcg tccaactgcg tcactcctaa
     4081 atatagtttg ctgatttcag tttatcccag tatacccatt ttttcaacaa aacaagtgat
     4141 tttcttttat cccagcgttt tctggataaa aaaatagata gaccataacg ggcctatcta
     4201 tttggtaacc ggtgctctcc tgccaactcc tcggtcacaa gtcacactac tcataaaaca
     4261 tgaaaagctg ttttttgaaa tcgtttcttg tgacttagag ttaaacggta ggcttacaac
     4321 ctcttcatta aacgtttttc aatcagctga ccttcggtat tagctcaata aacggcaaaa
     4381 tatgaggagc aatttttccg tttattgagc taatactcag gacaggacgc tgatttcaca
     4441 acctctagat aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgaaaa
     4501 aatgtggaaa gccattttca caaaattttt cttactactc gaaactaagc ggctttttca
     4561 caacctctta ttctgaaaaa taatttcctg gcacaaccgt aatatttttc gtttgagtat
     4621 ctacatcttt gacataaagc agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt
     4681 ttgattcggc aaatacagtg atgccaacaa gttctgattc gaattcttca atcaagctct
     4741 tcattccgtt gactgttccg ccgcctttca tgaaatcatc cactacaagg acacgtgaac
     4801 cacgtttcaa acttctctta gacagttcca tcttttcaat tctttcagaa gatccagaca
     4861 catagttgac acttactgtt gatccttctg ttatttttga gtcccggcgt acaataacga
     4921 atggcgcatt cagataatag gacacagctt gggcaatagg tacgcctttt gtggctacag
     4981 tcattacagc atcgattttt tcaccaaggt atttagaagc aatgattctt ccaacttgac
     5041 gcagcagttc aggttctcct aacaaatcag agagataaac atatcctcct ggaagcagac
     5101 ggtcttgttc tgatagacgt tctgctagag attcaatata ttttttcgcc tcttcatatg
     5161 aaatttcagg gatgaacaat acaccacctg cagcacccgg gattgtttcc aaggttccat
     5221 accctcgttc tttaaatgtt ttcttgatga ttcccaaatc ttcactgata gaagatttag
     5281 cagattcata acgttcggca aagcttgtta aggaaatcaa ttcatgcggg tgattcaata
     5341 aatactgtgt catgtcaact agacgttcac tgcgacgtac tttcacctat tccaactcct
     5401 tttaagaaat tttatcgttt tacattatat aagtttttct tcaaaaattc gagcatttta
     5461 tgaaaaaata agaaaatagt tcggtattta ttaaaataaa aagaagccta taaaaaattt
     5521 ttcgttttta taaggcttct tttctttata atttagttat tttttcttct gaaggcaacc
     5581 aatattcgtt tgatgatatt tacaatcagg aacaaagcaa taaagactaa tgtgattgtt
     5641 gctcctggag gtgtatctag ataaaacgag ctagttagtc ctgacagcat ccctagaaat
     5701 cctacacaaa cactgatagc aatcacgaca ttgaagcttt tgcctaatcg cataccgatt
     5761 gctgcgggaa gaaccatgat tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca
     5821 gcaatagcca caccagtaat cacattgaac atcatcgaca tcaaatgaac gggtagccca
     5881 tccacgtggg cggtatcttc atcaaaagtc agaacataca tcggccgttt gaataaaaag
     5941 aacaatatac cgatggcaac gaataagatt cctaaaaaga tgacttgctc ccatgtaatg
     6001 gtcacgatcg aaccaaacag ataggattgg atacttgttg ctgaattccc tccgctcaaa
     6061 ttcatcaata ctaacgctag cgccaaacca ccagccatta aaatggcaat ggagatttct
     6121 gaataggttc ggtagatcgt tcgcagatac tccaatatca cagctgctaa tacaacgact
     6181 agcatggtcg taagtgtagg attaaggttc aaaaagaatc ctaacgctac ccctgctaaa
     6241 gagacatgag aaagcgtatc agccatcagc gattgtcttc tgatcactaa aaacacacct
     6301 agcatcgggg cgattccggc aatgaagata gcggcaagaa acgctcttct catgaattca
     6361 tatgaaagca acgccattga gaatcctcct ttctcaccag tcggatctgt ctgtccgcat
     6421 actgtttgat atcttcatga tcatgggtga tcattagaac tgatttccca tggacatgcg
     6481 cactatgtcg gagaagacga taaaattcat ttctggattc ttcatccata cctgttgttg
     6541 gttcatcaag aacaaataag tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct
     6601 gcttttgtcc gccagacaat tcacctattc gccgatttcg catatcccac atccctacag
     6661 cttctaaagc acgctgtaca tgttgatgat ctttttcaga gagacgcttg aaccagcggc
     6721 ctcttggata tcttcctgaa cggactaatt cgatcaccgt actaggaaaa cctgcattga
     6781 aagatgcaac ttgttgagga atataaccga tacttaattt ttttccttct tgatttttct
     6841 cagcaatctt tatcattcct tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg
     6901 ttgacttggc tgctccattt tctccagtta aaatgacaaa ttcacccgca tctacatgat
     6961 aagagacatc ttctagtaca ggttcatcat catagtaaaa ggtcaaatca gatatttcga
     7021 tataacgcac cttacaacct ccattctaaa acttaattga tactttcttt taaagctttc
     7081 agattttttt ccatgacaga aatataattt tcgcctgctt tgatttgttc attagtcaag
     7141 ctttctaaag gattcaacac ttctagcttc actcctgttt cattcgacaa tgtctcagct
     7201 accttcgaag aggcattttc ttcaaaataa atcactttga tgttgttttt cttcacaaat
     7261 tcttttagct cagctaagcg acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc
     7321 gctacttgtt ttaagtcgta ttgattcgct aaataagcaa aagcagcatg ctgagtgaca
     7381 aatgtgcggt ttgttgcatc ttttagctca tcggagtatt tttgatccag tgcttcaagc
     7441 tttttgatat aagcatcggc attcttcgta aaagtttcct ttttatctgg atatttttcg
     7501 cctaattctt cagcaatcgt tctgacttct tttatagcta ataccggatc gagccaaaca
     7561 tgtggatcgt attcatgtga atgaccatgc ccttcttctg attcttcatg ttcgtctgct
     7621 tcttgcgatt ctaaacggtc gattccttta gctgcttcga tcatcaaggt ctgtttgcta
     7681 tccactgaat cctttaacga atccacaaaa aattccatgt ctgaactatt atacacgaaa
     7741 acatctgcat cggaaatttc tgctctttct tttgcacttg gttcgaaatc atgcggctcc
     7801 gttccagcag ggatcaaaag tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa
     7861 tcatacatcg gataaaaagt cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg
     7921 ttcgtatttc cacatgctgc aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga
     7981 tattttttca tttatatgct cctttcaatg aacaaatcgg aattttacga tttattaacg
     8041 tgaactaatg tatcaaaata aaaaaacaca gtcaatagaa atccatcaat ttctgattta
     8101 agcatgtata ttcttgatta aatcgtaaaa tggatgagtt gctgctcata tctagccatt
     8161 tcactacaat aaaaaactga agaataccat tttaggattc ctcagttttt catcacctat
     8221 tttacttctt atattttatt gcagggttcg tactaaataa acttcctcac aaaaaccttt
     8281 taacccatta tatactctct gcgctcttga ataatgttga cacaaggcaa aaacggttgg
     8341 tccgcttcca ctcatcaatg cagcatctgc tccgtacttg atcatacgct ctttgatttg
     8401 atggatgact ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat
     8461 catctgctga taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg
     8521 gatggcttgc gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt
     8581 gaccaacaca acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc
     8641 accgacaaat gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc
     8701 gatttctgcc agttcttcta acgaacagcc cagattccat aattggttga tccctcttag
     8761 agcagctgca cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt
     8821 aacaaagatc ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg
     8881 ataaacattg ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt
     8941 gtcttctgtt atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat
     9001 ttccaactca tgatacccat cttgccgtct atataataca tccagtccta aatttatctt
     9061 ggctggcgct ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag
     9121 tattctatca tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc
     9181 atcgtttcaa cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc
     9241 ttataattct gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat
     9301 cagactgagt agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata
     9361 acgtccttcc caatgatcga catattttaa tttcttttca taagcagact ggaaggaata
     9421 gaaaaaatta ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc
     9481 tgtttctcca acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt
     9541 ctcgtataca ataaacagat tggctaacag atagtcagct aattcctcag attctttagg
     9601 taagacccgc aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa
     9661 gccaacgact ttttgatctt tacggacaat accgacaccg ctagataata catatgattt
     9721 atcaaatcgt cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac
     9781 ttcatttaca gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc
     9841 ttctttcatc aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc
     9901 tagatcaaca atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat
     9961 cactacgtag ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg
    10021 tttcataaac tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa
    10081 aacaaagcat ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc
    10141 ttggtaataa aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc
    10201 gtatgtttcc aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt
    10261 atcttcagct aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat
    10321 aagccctgag aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg
    10381 accgactaaa aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc
    10441 gaacaagcta gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa
    10501 ttcttttctg gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca
    10561 agataacgta cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag
    10621 aaatgccttt ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac
    10681 caatagattc actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag
    10741 ccgactgatt gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa
    10801 atagacagca cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt
    10861 atctaaaaat cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat
    10921 aaaaggtaga acataataaa acaaccgata atataatagc caaactactg ctacatcttg
    10981 ccgaacagac aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc
    11041 tgggaccatc gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag
    11101 catcgaaata tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt
    11161 ccattgtcca aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa
    11221 caatgtcttt ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc
    11281 gaaaagccag atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat
    11341 aaatgtaaga aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac
    11401 agtggctaaa accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc
    11461 tcctaccaca ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag
    11521 aagaaactct tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac
    11581 ccaatcatac aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat
    11641 cgtcatgcgg ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc
    11701 aatatttgcg acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa
    11761 aatcagacta tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc
    11821 ccattcctaa cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt
    11881 ttccacttta gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta
    11941 aaggagagga taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta
    12001 ttgatggttt attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac
    12061 attttgaaaa tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc
    12121 aggaagttgc atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa
    12181 aagtattgga agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag
    12241 gaaaatcatt aatagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg
    12301 tgatgccgaa tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag
    12361 ctgccacgga ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct
    12421 atcctttagc agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat
    12481 tcgtttacat gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac
    12541 agacagccac tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa
    12601 gtgataaaaa tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg
    12661 caggtgtagt tgccttagaa gaagcaggat tcatcccagc aatcatcaaa ggaatcaata
    12721 cgacgatcga gaaagatctt gagatgttga ataaaaaata atgatagcaa tgttttttcg
    12781 tgccttacaa aaaaagaaga gctgtggctt ttgccgcggc tcttcttttg tattttttat
    12841 tatttagcat aatcagttgc acgagtttcc cgaataacga ttactttgat gtgtccagga
    12901 taatccagtt cttcttcgat tcgtttacga atatcacgta ctaaacgaac agcgtctaaa
    12961 tccgagattt cttctggctt gaccattacc cgaacttcgc gtccagcttg aactgcgtaa
    13021 cttgattcta caccggcgaa gctgttcgaa atattttcca gacttcttaa acgacggatg
    13081 tagttttcta atgattcact tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca
    13141 gctacaagga cagagataac agaagtagct tccacatctc catgatgaga agcgatcgcg
    13201 ttgattacta ctgggttttc tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga
    13261 gatccttcaa tctcatggtc aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt
    13321 gctaattgga tatcttcgcc aagctctgct gctaagatac cagctaattt tgcaacctcg
    13381 accgaatgct tcaagacgtt ttgtccataa ctagttctga atcgcagacg tccgatgatc
    13441 ttgattagat ctggatgaag tgtatgagca ccgacttcaa acgctgcctc ttccccgtat
    13501 tcgcgaatac gttcatccat ttctttacga gatttctcga ccatttcttc gatacgcgct
    13561 ggatggattc gtccatcttg tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg
    13621 attggatcga atccggaaag aacaactgct tccggtgtat catcgatgat taaatcgata
    13681 ccagtcagcg tctcaagcgt tcgaatatta cgaccttctc gtccaatgat acgacctttc
    13741 atttcatcat tcggaagggt aacgactgaa acagtcgttt ctgaaacttg atctgctgcg
    13801 cagcgttgaa tcgctaatga taataagttt ttcgctttgc gatctgcttc ttctttggca
    13861 cgctgttcag attcttttac cattagagtt aactcatgat tcagctcttc ttcagtcgat
    13921 ttcatgatga catctcgtgc gtcctctttt gataaaccag caattttttc tagttctttt
    13981 tgttgatctt caatcatttt ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt
    14041 gaactaagtg cttcttcttt actttcaagt gagttctcgc gtttttctaa cgagtcatct
    14101 ttacgattca gcgtttgttc gcgttgaatc aaacgattct cttgagattt taattctaac
    14161 ttactttctc tcagctcact ttcaacttca gaacggtatt tctgattttc ttccttagct
    14221 tctaacaatg cttctttttt tagagtttct gcttcttttt ttgcactatc gatgattcca
    14281 gcagctgatg attgtgcacc agctatttct ttctcgtgac gtgactttgc gaccataaac
    14341 cctaaaccaa gaccgacaat taaaccgatg atagcgagga gaatgttgaa taccataaaa
    14401 tccacctcca tactatcttt ttcattttta tcatctatac agcttctatg ataaattatt
    14461 ctaaactcgt caatatgcat cttcgcatat atgtgtacaa aaaacatgca caactttatt
    14521 ctaatgtttg taccccgtag tgtcaacttt aaataaacaa agaaacggct ttctacttat
    14581 tcattaaacg atggttttta aaataaatga cactagtttc atataggtaa acctttaaat
    14641 tgagaaactt ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac
    14701 ccactcgtat aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag
    14761 gagagtacct actcttcaga ccagccgttt ttgtttatca taatttttat tcttctacta
    14821 aaggcaattc ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat
    14881 aagctgctct aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga
    14941 ttttagcgtt ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc
    15001 ttttatcaac aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc
    15061 ctaaaccata catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta
    15121 cgactttgat ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac
    15181 gacgtacttc caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt
    15241 ctggattccc aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg
    15301 tttttgtttt gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt
    15361 gtaacccgac atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag
    15421 caactgaatc gactactacg atatctacag ccccacttga tactaaagca tcagcgatct
    15481 ctagaccttg ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta
    15541 atttttgtgc atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc
    15601 catttttttg tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag
    15661 gtccatatac ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta
    15721 acgcaagaga gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca
    15781 taatagagcc tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt
    15841 tacgatcatc tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat
    15901 tatccttatc atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca
    15961 tttatcattt tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat
    16021 atctgatagc agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag
    16081 atgaaatagc gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca
    16141 ctccggtgaa agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct
    16201 gctccacaca ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca
    16261 gaaaaccagc ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata
    16321 caccagcaat gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg
    16381 ttacagtctg cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat
    16441 cgccgtatcc ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa
    16501 gagcttgatt ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat
    16561 ttggtttggc atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct
    16621 cagtgaccag tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt
    16681 cttcagaagg aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca
    16741 actcattagg cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc
    16801 ctaaagcaag ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt
    16861 gcagattgtt tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt
    16921 atccttccgt attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca
    16981 ggtcatcttc tgtcggtcct aatccgccac ataagagtat gagttcactt cttgtttcag
    17041 ctagagcaat caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa
    17101 cttcgatacc tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt
    17161 gtcctagtaa aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc
    17221 ttatatagta tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg
    17281 acaaaaatct tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg
    17341 gtcataagtc acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga
    17401 ctcagagtta gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt
    17461 gaacacaccg ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca
    17521 agcataaagc atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat
    17581 aaacagcaag ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc
    17641 agccatgact tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg
    17701 acagacaatg atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa
    17761 agcagtcata actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac
    17821 aaggcctcgc gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa
    17881 aataactgca cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc
    17941 ccaatctaaa ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac
    18001 agttaattta tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta
    18061 tcagcgaagt ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt
    18121 tttattgaaa ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga
    18181 tccttctttt ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg
    18241 cgtattgaag agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg
    18301 gtacggattc tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg
    18361 catttgttgc tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt
    18421 gataaacata ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt
    18481 caagggttac aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt
    18541 ctcctgtagc tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag
    18601 aagactcttt ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta
    18661 ctttaagcga agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca
    18721 tatagcccac gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac
    18781 ttgtccaaaa gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta
    18841 ctgtttcagg ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa
    18901 catcgactaa atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc
    18961 gcacgtaaaa tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac
    19021 gtttttgaat ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg
    19081 cttgccgtaa tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt
    19141 tgattctccg aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa
    19201 agtcatgtca cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca
    19261 caagtcacaa tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg
    19321 gagttaaacg gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg
    19381 tattagctca aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact
    19441 caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg
    19501 cggtaataag tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta
    19561 ttacttgatg cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct
    19621 tcttcggcaa taagccgaaa tatcacaaaa atttgagaag caattttcgt aaattccttc
    19681 ttatttcttg aagctgacca cttctgtcac aacctctttg attacttcgt ttgattttat
    19741 tttaatataa gttgattaaa agagagcatg atttgtttta ttttctcact aatttttcca
    19801 acacaactaa gaataccata aaagctgatt ttggaggagg ttcctactac aagtctaaaa
    19861 cgaaatttaa gacttttcct cataaaatac tggtttattt tggtctcatg taaaactcgc
    19921 tgaaggcctc ttcacttata aatgcttccc cagctgccag tacgtctttc atttggatcg
    19981 aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt
    20041 gattggcaat atattcgatc gaatttaatg attggaaata ttgtccaagc attttcttct
    20101 tcagcaaatc gagatttgtt tctgacactt ccggatcatc agcgaatcca agaatgattt
    20161 ctttgacttt ttctgctgct ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt
    20221 gaaattcacg atccaagctg aattcaaaac caaagctgtc atcgataatt ccttgattgt
    20281 acatcgctag atagtttcga gaagtattgc ctaaaatcat ttggaacaac agattgattg
    20341 ctgttttata gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat cctttaatac
    20401 caagtacaaa tttatctcgt gtaatggctg cttccaatgc actttgtttg atgatttctt
    20461 ttgtgttttc agggaaataa cgtacaattt cttgctttgg agggaaattt tttgcttctt
    20521 ggttttcccg aataagtttc atcaatttct caggctccat cttaccaact acaaataaaa
    20581 ccatattgct tggctgataa aacgtccgat agcacgtata caagtcttga gctgtgattt
    20641 tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt
    20701 tatttagtat gccaaaaaac atccgccagt tagggtcgtc ttcatacatt tggatttcct
    20761 gaccgatgat tcctttttcc ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa
    20821 aatcgatcag agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac agatagctgg
    20881 ttttcgtaaa acttgtgaaa gcattggcgg aagctccttg tttcccaaat aattgaaaga
    20941 catcaccatc ttctttttca aacaacttat gttctaaaaa atgggcaatt ccatctggaa
    21001 ctttcacctt ttctttttca ccatagggaa taaattcgtt atcgatcgaa ccataattcg
    21061 tactgaacaa accatatgtc ttgtgatagt catttttagg caacaaatac acggtcaaac
    21121 cgtttggcag tacttcatga taaagtgttt cattgatttg ctcatattct gttttattca
    21181 tttgcatttc ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc agctacttgt
    21241 tgtatctctt tgatcgttac gctctccata cgtttcaacc attcttcatc agataatttt
    21301 gtttccggca gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga
    21361 gaaagcagat attggtttct cagcattgct tttgtctgag acagttccaa ctctgttatt
    21421 tcaccatttc gcaaactttc taattgctcg tgaatcagat gaagtacttg atttcggttc
    21481 ttttcatcga taccggtttg gacactcatg aatcctcgga acgtatccac gctactagag
    21541 gcataataag ctagactttc tttctctctg acattcatga aaagtttaga gtgaggaaaa
    21601 ccgccaaata aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc gtaatacaca
    21661 tttgtctgat aagctaggtt cagctttgct tgaacgattg gctcacgtac ttgtcgttcc
    21721 ttcacgatat tcacttgagg ttgggtataa aacagatctg gataaactgt ttcccgcgac
    21781 tcaaatggta attgcccaac tgcttcagct gctttgtccg gatcaacatc gccgacaaca
    21841 aaaatgtcga tttgatcatt tgccatcatt tcttgatagt aggacgctag cgaactagca
    21901 gtgatcttgt ccaaatcttc gactacgccc aaccctggga tcttttgttc cgggctgttt
    21961 tcaaaataaa gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc cttcaaactt
    22021 tccaaatata gacgcagatt gttcttttcc aagtcaaacg tcaactgatc aaactgatga
    22081 tttttaatat ttggataaaa taaaacctct tttaaaaaat ctaccgcttg agaaaaaagg
    22141 tttggatcat tgacatattc accattcaca aaactgatcc ctgcgtttag ccaatgtaaa
    22201 tttccttttt ttctaactga taatccaaag cttgcgccat aaagctctgc aagttgcgca
    22261 cttaactttg tctgatccga ataattcaaa ctatttgttt ccaacatgct gctgagaagt
    22321 gaacgtttcg tgatgacttc ttgttgcaga cgcgcagtaa aacggataaa aatccggatc
    22381 gttttgtatt gttttgttgg taagacatga agattcacgc cttctgccaa ttgatacacc
    22441 atatttatat gctccttttc acagttgatt gcccgcttac tcttttaatg ggtcaacctg
    22501 ccttttttac ttattcctct gcttgaaatt tataagttca ggcaattcgc cgttttttct
    22561 aaatgatgtt acactttata gtatagcata gatgtttttt atcaaaagaa acagacggct
    22621 aaaagcgtat attgtttttt tattttttat ttttcgatat actaaagaca aataaaagaa
    22681 gggagggtca ttcatgatca aagaattcaa agagttcatc atgcgtggag atgtcttgga
    22741 tcttgctgtc ggcgttgtta ttggtagtgc gtttacaagc atcgtaaacc aagtggtgca
    22801 aggcttgatc acaccactaa taggttggtt tgtcgcaatg atcacaggta cttctgattt
    22861 agaaggagca ctttcaattc ttgactggtc gccgacaaaa ggcgtcacat ttgcgtttgg
    22921 taacgttatt agtgctatca ttacatttct gatcactggt tttgtcttat tcttagttgt
    22981 caaagcggca aaccgtgcga agaatttacg ctccaaagaa gagcaagtgg aagtggaaga
    23041 aactcctact gcagaagatt atcttagtga catacgcgat cttcttgcca aacaagtggc
    23101 agaagacaat acacatccag tagagcaaac aaatctttcc gatacttcta aataataaag
    23161 aatctaaaaa aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact
    23221 tgctacttgt tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga
    23281 gattgccttg aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac
    23341 gattggtttt tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct
    23401 tcctaaacca tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc
    23461 cgcttcttca tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt
    23521 atgccaagct tctccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac
    23581 aaaaactgcg attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa
    23641 tttctttacc tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg
    23701 attcgcacac aaaaaagcaa acgaacctta cgaatctcag gctcgtttgc tttttaaact
    23761 gcttcagtta aaaattcgat ctcaactgtc cgagtaagta catctgaata gctgtaagat
    23821 actcgttcaa aagaattttc ttcaggatcc aaatcaacaa cgaatactga aggataggtt
    23881 tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc ctgtctgagc aaccaacatg
    23941 attgggctgc cgatacgacc ttctaaatct ttcttgattg aagctaaagt tgttggcata
    24001 cagttcacct ctactatcaa ctataccaca tatttttaaa attttcaagt ttaccataaa
    24061 aaaaaaagaa atgcaataaa aataaataag aagctcatta agatttttct aaattattcc
    24121 tcattataaa atttcaagtg tttttttatt ttgagtttta ctctcccgta agcgctcatt
    24181 acttgcttgt gatccattgc taaacattca gccatgtcta caaaatcaac gccttgcata
    24241 taataatgaa aaattttagc ttctattttt gaaaactgga tttcgcaatt ttcgattgct
    24301 tcttttaata aaagatgctg aattgctaaa ggttcttgca atgtgatctc tgctaaaaag
    24361 tctggtcctt ctgtctctat tttttggtcg attgagactt catctttttg atcacgacgc
    24421 ttcagcgcgt tctgcttcct caacagagaa tagatttgat tgcttataac gcttttataa
    24481 taacttccaa agctaacacc tttgcttttt tcgtaagttc tcaatgcctg atataaagcg
    24541 atccttgctt cttgttccca atcctcagaa acataatttc ttaaataata ggtgtttttc
    24601 aaacgtagta aaagtggaaa atatttttga tacagggcag caaatgcttc ttcgtcacca
    24661 ttacgtgcag cgcgcaataa ctcctcagac ataaaataat tcctccataa attcgtttat
    24721 ggaggaatct tatcatcaat gattcatcga actgattaga acgatatgtt atttattttt
    24781 tctcattaaa tcgtccaatt tttgggacaa tttttttaac tgctcttcat tccatggaga
    24841 attacgtcgg aaatcaataa aatgcatatc tttattttct tgggctattg ttttttccgt
    24901 tttttttatc gtcttatata attcataagc agaagttcgt aatgctcctt gggaaaagat
    24961 catccactgt tctgctaaat cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag
    25021 ctgaccagcg attctctcga tataactgtc agccgtttcg tcttctttcg taaaaacaac
    25081 atccaactgg tatttcttat aattttgttg gatgcccgga actagttggg catcgaagac
    25141 aacgatgact tccattcctt catatttcgc ataatttgat aaacgatgta aaagagcttc
    25201 tcttgcatcc gccattttgt cctgcttttt aagctggaca agttccggcc acgaaccaat
    25261 catgttgtag ccgtctacaa tcaacagctg ttttttcatg gtctactccc cctgacgttg
    25321 ggaaaaagcc tgatacataa gcaaaccagc agcaacactg gcatttaagc tctggacgtg
    25381 cccggtcatc gggatcgtca gcaattcatc catttctttt ttcaatcctg gactcattcc
    25441 tttgccttca ttaccaatga ctaaagcaat tgatcctttg gcattccatt tttggaagtt
    25501 tgtgccggtc atatctgtgc caaataccca aaatcctgtt tctttgagct gttgcacagc
    25561 ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg actgccccag tggaagtctt
    25621 tgttacgata ggtgtgattc caactgctcg gtgttttggt atgatcacac catcgacgcc
    25681 acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa
    25741 aatcaaaaag aaggggtctt cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga
    25801 taaatattca taaggtgtaa tcgcaagaac gactccttga tgcacgccgt gatcgctcaa
    25861 agtttctaat ttttgtttag gaacccattt gactggcaca gcttgttcac gtgcgatttc
    25921 tttcagttct tctacttttt ctcctcgtga atcttcttga agaaagagtt tattccctcg
    25981 cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag acgaaattat cttctaatgc
    26041 ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt tgctttttat ttgaatatct
    26101 attttgtttt ttgtcttcaa atccacgttt cgattgatgt ttgtttgttc tttctttacc
    26161 catttttttc accaactttc tgaatacacc agcggatcaa ttcttccatc cgctcggttt
    26221 gttttgtcaa atgcagatag cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg
    26281 taacatcagc attttttgcg ctcgtatggc ttttagcatt tcttcctctg cgataataaa
    26341 gttcttcttc ttctgtcaaa agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg
    26401 cattcgcaga aacataattc gttgccgcac gatgcagctt attgggtttt gtttctcctt
    26461 gatcaatcag gtggtcacga acatagattt cgtaaatcgc atctccaaca taagccaagg
    26521 ctaaaccgtt caattgtgtg taatctctca tgcttctctt ctccatcggg ttccttgcgg
    26581 tgtatcttct aataagatcc ctttttcttt caacaaatca cgaatttcgt cgcttcggac
    26641 aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc aattgttcaa tctcttcatc
    26701 taataactct tctgctgtaa ataaaatacc gaagatcgaa agccattgcg agaataaagc
    26761 ttctccttgt gccaagacag cggcagaaac tttcggctgg tctagataag tgttcagcca
    26821 cttagcaagc tcatagacca ctgtgatccc atttgctgca ttaaaatcat cgtccatttc
    26881 ttcgatgaat cgctgttcta aattttctaa caaagcgatt ttatccgcat catctgccaa
    26941 attctctgag gcagtttctt ggcggaattt caaattgtca aacgtattct tcagtcgttg
    27001 taaattggtg gctgcttctt tcatcgtcgt ttcactgtag cggatcggac gacggtattg
    27061 tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg tttttgatca tttcatgtac
    27121 agtgatgaaa ttccctagag atttactcat tttttcatcg tcttcgccaa tcgttacata
    27181 gccattgtgc atccagtaat tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat
    27241 ttcattttca tgatgaggaa attctaaatc ttgcccgcca ccatggatat cgatcgtttc
    27301 tcctaaatgt tttgttgcca taacggaaca ttcaatatgc caaccgggac gtcctttgcc
    27361 ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt ttccataaag cgaaatctaa
    27421 tgggtcttct ttttgctgct gttcttcccc tgtacgctga cttgctccga cttccagttc
    27481 atcgatggat tggtcactta atttcccata attttcaaat ttacgggtac ggtaatacac
    27541 atcacctgaa gattcataag catagccttt ttcaaccaac acttgaataa atgctaaaat
    27601 atctggcata tgatccatga ctcttggatg cagtgtagca ggttcgacat tcaacgcttt
    27661 cgtatcttct tcaaatgctc ggatgaaacg atcagcaact tctggtgctg taatacctaa
    27721 ttcgtttgct gtacggatga ttttatcgtc tacatccgtg aaattcgata cgaaatcgac
    27781 tttatagcct cgatattcaa aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc
    27841 atttccgata tggatataat tatacacagt cggtccgcag acatacatac cgacatgtcc
    27901 cttttgtata gggatgaatt cttctttttt tctggtcaat gtattgtaga tttttatcat
    27961 tctgatctcc tcactttttc tcctttgatt cgaacgactt ttgctggtac tcctaccgct
    28021 gtagcgtcgt ctggaacatc tttcaacaca acggcacctg caccgatttt ggcgtttttt
    28081 ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca
    28141 gttgggtgtc ttttacccgt ttcttttcct gtgccgccta atgttacgcc atggaataaa
    28201 atcacatcgt cctcgatttc tgcggtttcg ccaatgacga ttcccattcc atggtcgatg
    28261 aacacgccag ttccaatcgt tgctcctgga tggatctcaa caccagttag gaaacgccag
    28321 aattgagcat tgatcttcgc caataaaaag agccggtgct gatataaaaa atgagagaaa
    28381 cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt
    28441 gcagccggat cgtttctctt tgctgcatca atcgctcggg taaaccaact catgactttc
    28501 cactcctttc gtaagaattt tatgtaggaa atcatcttct tataaagaaa agaagcgcct
    28561 tttaaataag tttccttatc aaaagacgct ttccgcgtgg ttccactttt cttcataatc
    28621 aatgattatc tctgacagct aacgcctgtt atacgttctt ggctactttg tttcctataa
    28681 aacagttttt cctgttttct gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc
    28741 attcattgat accttgcacc acccggtatc tctcttgtaa tgatcagctg cttacttttt
    28801 tctgttcatc gtgtttagtt cagtacttga tttaaatgtg ctaatgattt ttcacgacca
    28861 agcaattcga tcgtttcagg caattctgga ccatgcattt gtcctgatac agctacacgg
    28921 attggcatga aaagattttt tccttttacg ccagtttctt tttgaacagc tttgatggca
    28981 gctttgacag ttggagcatc tactacttcc atttgttcta gctgtgcttt gaatgcgtgg
    29041 agaacagtag gaactgtttc accagcaagt acttcttttg ctgcttcatt caatacagga
    29101 tgttcattga aaaatagcgt agacagatca acgatttctg cagcatagct catttgaggt
    29161 tgatacaggc taacgatctt cttcacccat tcgattgtct tttcatctgg gttttctgct
    29221 acacgtccat cagcgattag ataaggcaga cacatgtcag taaatgcatc tagatccatt
    29281 gctttgatgt aatggttgtt tacccattgc agttttttcg catcgaaagc tgcgggtgat
    29341 ttacttaggc gttttggatc gaacattttg atcaattctt ggtgttcgaa gatttcgtct
    29401 tcgcccacag gtgaccagcc caataatgca ataaagttga acattgcatc tggtagatag
    29461 cctaattcgc gatattgttc gataaattgc aagattgatt cgtcacgttt gcttagtttt
    29521 ttacctgttt cactgttgat gatcaatgtc atatgtccga aggttggtgg tgtccagcca
    29581 aatgcttcat agatcatcag ttgttttggt gtattggcaa tatggtcatc tccacgtaaa
    29641 acgtgagtga ttttcatcaa atgatcatcg accgcaacag caaaattata agttggcata
    29701 ccatcgcgtt tttggatgac aaagtcgcca ccgatattat cggattcgaa cgaaatagcg
    29761 cctttcacca tatctgtaaa tgcgtattct gtattgcgag gcacacggaa acgtacaaca
    29821 gattctagac cttgtgcttc tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt
    29881 cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt cccgttctgc ttctaattct
    29941 tcttctgtgc aatagcattt gtatgctcga ttgctagcaa gcagctgatc aatcagcggt
    30001 tgatagattt ctttacgttc agattgacgg taagggccgt attctccagg attttcagga
    30061 gattcatccc aattcattcc caaccaagca aggttttcca actggctttt ttcgccatct
    30121 tcgatattac gtttttggtc agtatcctca atacgaataa taaagtctcc atcattgtgg
    30181 cgggcaaaca aataattgaa taatgcagtt cttgcatttc caatatgcag gtgcccagtt
    30241 ggacttggtg catagcgtac gcgtactttc gtcattttct tcctcttctt tcaaactaga
    30301 ctttgactaa cagcaaaaat tccgtcagtt acggcttctc aaatatcatt tatttaaagc
    30361 cactcagcac aaattttacc ttttctaagg taaatagtaa agccaactgg ttgaatttcc
    30421 ttaccagttg gctgaatttc ttttttattt tgattgttta gtctttttct ctgtctcttt
    30481 tttattgtcg attcctcgtc ctgcatgtgc aggtttagca aagatcatgc gtccagcagc
    30541 tgtttgaagg gcacttgtta caacgacttc gatacgttca ttcatgtaat gctgtccatc
    30601 ttctacgaca accatcgtgc cgtcatcaag gtaggctact ccttgttggc gttctgtccc
    30661 ggcttttact actagaacat tcatcgtttc accagggatg acgactggtt ttacggcatt
    30721 tgccaacgca ttgatattaa gaacaggtac attttggaat tcagagactt tattcaagtt
    30781 atagtcattg gttacaatga caccatctaa aagcttagcc aatttgatca atttgctgtc
    30841 tacttcactg atatcttcaa agtctccatc gtacatttcg acagatatac cctcttcttt
    30901 ttgcaaggca tttaggatat ctaatccgcg acgaccacgc acacgtttca aactatcccc
    30961 tgagtccgcg atgtattgta gttcgtaaag aacaaaatta gggatcatca aggttccttc
    31021 taagaatcca gtctttgtaa tatcataaat ccgaccgtca atgatcacgc ttgtatccaa
    31081 gattttatat ttatggaaat tttctcctga ttttcgttct aacagttgac tctcaacttg
    31141 actagctgtt tcttgctcga tttttttgct tcgtgaacca aaaactttct tccattcttc
    31201 gattcgtgtc gttcccattc tcaatcccag atagcctaag atgatcatca gtaaaatcgg
    31261 caatacacta ttgacgaatg gaatttccat attgtaaaga ggggtcgaga tcacaacacc
    31321 taaagtcaat ccgatgatcg caccgatcgc accgaagaga agataagtca agctgacctc
    31381 acttagtttt tgttctactt gtttgataat agctgagata tgtttggcca acagcaaaga
    31441 taataaaaag aaaataagtg cgcctaacag gctgttggta aatgtattat tcagccatcc
    31501 gtttgactgt tgaccgattg cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc
    31561 gccgacaatg atcatcagca aagtaatgac acgtttttgc atcgtttttc ctccgtttcg
    31621 acaatcattt attagttagc ggaacacacg cttcaacgtt tcagccaaag ttgctactcc
    31681 aactatctcg attccttttg gcgcttccca gttacctaag ttgtttttag gtaggtatac
    31741 ttttgtgaag ccgagttttt ggacttcttt gactcgttgt tcaatatgac tgacacgtct
    31801 gatttctcca gtcagtccaa tttcaccgat gaaacattcc gttggctgtg ttcctttttc
    31861 tttatagctt gaagcaatac tgacagcgat agctaaatca atcgctggtt catttaattt
    31921 taccccacct gcagctttta agtaagcatc ttgattctga agaagcaacc cagctctttt
    31981 ttccagcact gccatgatca gagatacccg attgaaatca agacctgttg ttgttcgttt
    32041 ggcatttccg aacatagtag gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt
    32101 cccttccatt gccacaacga tagcagagcc agttgcaccg tctaggcgtt cttctaagaa
    32161 aatctgtgaa ggattagcga cttcttccaa tccatgctca tgcatttcaa aaatcccaat
    32221 ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa atccgaaaac tatgatgttg
    32281 ttcaccttca aaatataaca ccgtatccac catatgttct aacattcttg ggccggcgat
    32341 cgagccttct ttggtaacat gaccgacgat aaaaatggct atcccatttg ttttagcggt
    32401 tttgagtagt tctgcagtgg tttctcttac ttggctgaca cttcctgcca cacttgtgat
    32461 gtctggctgc gtcatcgttt ggatggaatc aatgataaca taatcagggg agatatgttc
    32521 gatggcacga ctgatttcgt tcatatctgt ttcagcgtat agataaaatt ctgtgttgat
    32581 acttccgagg cgttccgcgc gtaattttat ctgctctgcg ctttcttcgc cggatacata
    32641 taagactttt ccgcctatgg ctgctaattg ctgagaaact tgtaacagca aagtggattt
    32701 cccgatacct ggatcgccac caatcaagac catagaccct ggcaccacac ctccgcctaa
    32761 cacacggttc agttcttcta attttgtttt gatccgaggt tcttttttag ggacgacgtc
    32821 tgcgattttt gttggttttg ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt
    32881 tgtatcttgt tcgatttctt cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc
    32941 caagaactta ggcgacacat acccgcaatt ttggcaaaca aattgtactt ttgatttttt
    33001 tgccattgta ccgcctcctt ttatcctaat catgagacat taaatcaagc ttaagtatct
    33061 ttaacgtttt atttactgga agaaccaaat ccgccttgac gttgagcagt cgtttgatct
    33121 tgatctgctt ttaaaaacgg caaaaagatc ccttggccga tacgctcgcc ttttttgatc
    33181 actacatcct tgaaaccaaa gttggtgaat tggaacatga tatggccttc atttgcctca
    33241 ttattgtaat aatcactgtc aatcacacct actccgtttg tcaacactaa aaaccgcttc
    33301 aacggattgc ttgaacggtt agcaagctgc aaaaattcat cttctcccat gtaagctttg
    33361 attccagtag gcacgagggt tggtttcaat tgtttttcat tttctgatac atcttttttc
    33421 aatccatctg cgactgtttc caaccccttt ttccagcctg ctttccagat acttggaatc
    33481 acgacctctt ctgctgcttc aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt
    33541 agattgatat tttgatcttt atatttagaa ataacttcaa atcctctttt tctcaccggt
    33601 tttcctccct aaataaacta ctcctattct aacatatccg aggctgtaaa agactttctt
    33661 tacaaattct tcaagaaatt ctgctatctt aaagtaaacc attaattcgt tctttttcca
    33721 aacgactgca agttgtacaa aaaaactatt ttttacatga ctttactcgt caaaacaagt
    33781 agaatagaac gaaattaatg agtggatatt tgattgaggt gtcacatgcg taaagaaatt
    33841 aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta cattgattta tcccaatgga
    33901 aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc tttctgatct tagaaatcgg
    33961 ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg ttggtatgca taaattacat
    34021 ttgtcagaac gtccgacttt gattcctgaa cagcaagctg cggcggctgt tggtcaagcc
    34081 gaactgatga atatctataa tcaaagattc agtgtatata atcaacagac ggcacagatc
    34141 ctcgtgacaa gagacgtggt agattatcct gaaagtcgta aaaatgtgag gaatacattg
    34201 gaacagcttt tgattatgga cacgatccct atcgtgaacg aaaacgatac tgtagcagtg
    34261 gatgaattag atcatctgac aaaatttggc gataatgatc agctgtcagc catcatggca
    34321 aaacttgtcg cggcagacct tttgatcgtc ctttctgata tcgacgggtt ttattcagat
    34381 aatccgacta caaatcccga ggctcagatg tatcgctata tcgataaaat cgatgaaaag
    34441 attctggcac aagcaggtgg tgctggtagc acatttggaa caggcggtat gcagagtaaa
    34501 ttaaaagccg cttctaggat atttgaagca aacagcgcaa tgattttagc aaatggaaaa
    34561 aatccggcaa ttatttttga tattcttgca ggaaaagaag taggtacttt atttaaggag
    34621 gacttgaaat gactgacttg atgcaattag gacaacaggc aaaagatagc gcgcaacaac
    34681 tagcacttat gcctacaaac cgcaaaaatg agctacttgt tcaaatggca caaaccatca
    34741 aaaagaatca acaagcgatc attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa
    34801 acaatatcag tgaaacgatg caagaccgct tgcagctgac acctgagcga atcaatgcca
    34861 tggctacaga gatcgaaaaa attgcttcgt tagacgatcc tattggaaaa gtagatgaga
    34921 tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg
    34981 gcatcattta cgaatctcgt ccaaatgtca cgacagatgc agctagtcta gcattcaaat
    35041 cagggaatgc cgttatcctt cgcggtggca aagaagcatt cttctcgaat caattattag
    35101 tccaattatt gcagcaagta ctggtcagtg ctggcgaatc tccttatgcc atccagtttg
    35161 tcgatgatac ttctcatgaa actgcaggac agttgatgcg attgacagaa tacttagatg
    35221 tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt caaagaacag gcaacggtac
    35281 ctatcattga aacaggtaca ggaaataatc acgtatatat cgataaagaa gcacagttat
    35341 ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg
    35401 cagaaacatt gctgatccat tctgagatcg cacctttttt cttgccagct attgaaaaag
    35461 aactggtaga acacggggtc tctttacgtg cggatgctcg tgcgttagaa tatctagaga
    35521 cggctgcttt agcagaagac acagattggg atactgaata tctagattat atccttgctg
    35581 tcaaagtggt cgattctttg gatgaagcaa ttgcacatat caatcggcat aatactaaac
    35641 attcagaaac gatcgtgaca gatagttatg cggctagtca acgtttctta aatgaagtgg
    35701 acgcggcggc tgtctatgtc aatgcctcca ctcgttttac agacggatcg gtatttggtt
    35761 ttggtgcaga gattggtatc tctactcaaa agttgcatgc ccgtggtcca atggggttaa
    35821 atgaattaac ttccacaaaa tatatcattt atggcgatgg acaaatccgc gaataatgtt
    35881 tcgtgtgaaa cattattccc gccttttgcc ttttttctgt aaaggttcta tccaactaga
    35941 aaggtgtcaa tatgtttgaa attttaatga ttttttttat ttaccttatt tctctgaata
    36001 tcgctgcttt tctgggagtc ggtattttat cccttttctt tcaattcaaa aaacgaagca
    36061 tagggagtca acgggaaaaa tggtcgcagt attttgacaa aatcgggcaa aaagggctgg
    36121 ttacacgctt gcacattagt tacatggttg cattatgctt attagcgaca atcaattact
    36181 attctttttt cgatcattca atcgcttata cgattacgct actgattgct gggatttttc
    36241 acttatctta taaatatcag ctgaataaaa accacttgaa tcggactttc cgctaaaaaa
    36301 tatccgtacg ataagtagag tgccttgaag atcggcaaaa aactgccgga ctttttagag
    36361 gctgtgacaa agattttgtc acagcctctt tttccatata aacggcgttc gcctaccagc
    36421 tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat
    36481 cataataacg aagcagctca tccatggaca agttcatcgt tttttctttt tcttcttccg
    36541 ctgattcagc cagtagctgt tcccgataca cctgatacat accagctatt ttttgctgca
    36601 tcgcttttct agtttcacct tccgcattga ttattggcaa ttctttgata tagccacgat
    36661 aaatcaaccg atcatctgaa aattcaatgg gtaattcaac gagtgtcaac ccgacttcct
    36721 gaaaatattc ttccagcatc tattatcccc ccattctttt tcttaaatga attttagctt
    36781 acctactgaa aattttccag tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc
    36841 gtgtcacatc ccctttttcc gaataaatgg tgttcttctg ccaactcctc aatcataagt
    36901 cacaatattc aacaaacatg agaaactgtt ttttgaaatt gttccttgtg attcagagtt
    36961 aaacgacagt ctcacaacct cttggtaaac ggcgttcaat cagctgaccc tcggtattag
    37021 ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt tgtttctcta atacttagga
    37081 taaaacgctg attttacaac ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta
    37141 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta atactcagga
    37201 caaaacgctg attttacaac ctcctg
//
LOCUS       DADOOS010000012        34825 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.012, whole genome shotgun sequence.
ACCESSION   DADOOS010000012 DADOOS010000000
VERSION     DADOOS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.012"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            156..1862
                     /locus_tag="J8C37_000523"
     CDS             156..1862
                     /locus_tag="J8C37_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAZ5303984.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="J8C37_000524"
     CDS             complement(2020..2922)
                     /locus_tag="J8C37_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ5303985.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="J8C37_000525"
     CDS             complement(2919..3623)
                     /locus_tag="J8C37_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAZ5303986.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="J8C37_000526"
     CDS             complement(3654..4295)
                     /locus_tag="J8C37_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ5303987.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="J8C37_000527"
     CDS             complement(4333..4602)
                     /locus_tag="J8C37_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5303988.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="J8C37_000528"
     CDS             complement(4575..5264)
                     /locus_tag="J8C37_000528"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAZ5303989.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="J8C37_000529"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="J8C37_000529"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAZ5303990.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="J8C37_000530"
     CDS             complement(5984..6916)
                     /locus_tag="J8C37_000530"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAZ5303991.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="J8C37_000531"
     CDS             complement(6913..7689)
                     /locus_tag="J8C37_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAZ5303992.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="J8C37_000532"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="J8C37_000532"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAZ5303993.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="J8C37_000533"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="J8C37_000533"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAZ5303994.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="J8C37_000534"
     CDS             complement(12014..13294)
                     /locus_tag="J8C37_000534"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAZ5303995.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="J8C37_000535"
     CDS             complement(13313..14239)
                     /locus_tag="J8C37_000535"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAZ5303996.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="J8C37_000536"
     CDS             complement(14252..15535)
                     /locus_tag="J8C37_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAZ5303997.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="J8C37_000537"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="J8C37_000537"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAZ5303998.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="J8C37_000538"
     CDS             complement(16323..17234)
                     /locus_tag="J8C37_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAZ5303999.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="J8C37_000539"
     CDS             complement(17231..17695)
                     /locus_tag="J8C37_000539"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAZ5304000.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="J8C37_000540"
     CDS             complement(17700..18398)
                     /locus_tag="J8C37_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ5304001.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="J8C37_000541"
     CDS             complement(18385..18771)
                     /locus_tag="J8C37_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304002.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="J8C37_000542"
     CDS             complement(18918..20588)
                     /locus_tag="J8C37_000542"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAZ5304003.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="J8C37_000543"
     CDS             20771..21004
                     /locus_tag="J8C37_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAZ5304004.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="J8C37_000544"
     CDS             complement(21103..21534)
                     /locus_tag="J8C37_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAZ5304005.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="J8C37_000545"
     CDS             21613..22026
                     /locus_tag="J8C37_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAZ5304006.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="J8C37_000546"
     CDS             complement(22064..23212)
                     /locus_tag="J8C37_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAZ5304007.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="J8C37_000547"
     CDS             complement(23334..23930)
                     /locus_tag="J8C37_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ5304008.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="J8C37_000548"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="J8C37_000548"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAZ5304009.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="J8C37_000549"
     CDS             27160..27591
                     /locus_tag="J8C37_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5304010.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="J8C37_000550"
     CDS             complement(27636..28985)
                     /locus_tag="J8C37_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAZ5304011.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="J8C37_000551"
     CDS             complement(29055..30218)
                     /locus_tag="J8C37_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304012.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="J8C37_000552"
     CDS             30347..30670
                     /locus_tag="J8C37_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304013.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="J8C37_000553"
     CDS             complement(30853..32202)
                     /locus_tag="J8C37_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5304014.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="J8C37_000554"
     CDS             complement(32271..33224)
                     /locus_tag="J8C37_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAZ5304015.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="J8C37_000555"
     CDS             complement(33326..34516)
                     /locus_tag="J8C37_000555"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAZ5304016.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9905 a         7619 c         6105 g        11196 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaat ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caata
//
LOCUS       DADOOS010000013        34424 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.013, whole genome shotgun sequence.
ACCESSION   DADOOS010000013 DADOOS010000000
VERSION     DADOOS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.013"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(76..543)
                     /locus_tag="J8C37_000556"
     CDS             complement(76..543)
                     /locus_tag="J8C37_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAZ5304017.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="J8C37_000557"
     CDS             complement(663..2201)
                     /locus_tag="J8C37_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAZ5304018.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="J8C37_000558"
     CDS             2281..2967
                     /locus_tag="J8C37_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAZ5304019.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="J8C37_000559"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="J8C37_000559"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ5304020.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="J8C37_000560"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="J8C37_000560"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAZ5304021.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="J8C37_000561"
     CDS             complement(4453..5583)
                     /locus_tag="J8C37_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ5304022.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="J8C37_000562"
     CDS             complement(5583..7766)
                     /locus_tag="J8C37_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAZ5304023.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="J8C37_000563"
     CDS             complement(7763..8788)
                     /locus_tag="J8C37_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ5304024.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="J8C37_000564"
     CDS             complement(8833..9660)
                     /locus_tag="J8C37_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ5304025.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="J8C37_000565"
     CDS             complement(9657..10724)
                     /locus_tag="J8C37_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAZ5304026.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="J8C37_000566"
     CDS             complement(10750..11586)
                     /locus_tag="J8C37_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304027.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="J8C37_000567"
     CDS             complement(11583..12452)
                     /locus_tag="J8C37_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304028.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="J8C37_000568"
     CDS             complement(12522..13805)
                     /locus_tag="J8C37_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304029.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="J8C37_000569"
     CDS             complement(13819..15504)
                     /locus_tag="J8C37_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAZ5304030.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="J8C37_000570"
     CDS             15779..16804
                     /locus_tag="J8C37_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5304031.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="J8C37_000571"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="J8C37_000571"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAZ5304032.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="J8C37_000572"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="J8C37_000572"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAZ5304033.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="J8C37_000573"
     CDS             25476..26444
                     /locus_tag="J8C37_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304034.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="J8C37_000574"
     CDS             26462..27367
                     /locus_tag="J8C37_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304035.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="J8C37_000575"
     CDS             27364..28170
                     /locus_tag="J8C37_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5304036.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="J8C37_000576"
     CDS             28327..29295
                     /locus_tag="J8C37_000576"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAZ5304037.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="J8C37_000577"
     CDS             complement(29612..30136)
                     /locus_tag="J8C37_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAZ5304038.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..32006
                     /gene="scm"
                     /locus_tag="J8C37_000578"
     CDS             30423..32006
                     /gene="scm"
                     /locus_tag="J8C37_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAZ5304039.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTS
                     STTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGV
                     ILLVIGLRITFMSYKKSKR"
     gene            complement(32161..33147)
                     /locus_tag="J8C37_000579"
     CDS             complement(32161..33147)
                     /locus_tag="J8C37_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAZ5304040.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(33170..33736)
                     /locus_tag="J8C37_000580"
     CDS             complement(33170..33736)
                     /locus_tag="J8C37_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAZ5304041.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(33750..34166)
                     /locus_tag="J8C37_000581"
     CDS             complement(33750..34166)
                     /locus_tag="J8C37_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAZ5304042.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
BASE COUNT         9730 a         7419 c         6473 g        10802 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    31501 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    31561 cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt gacagtacag
    31621 atactacctc taccacaagt gatagctcag attcatctac cactacaagt gatagcacgg
    31681 acacgtcagc ttcaagtgac agtacagata catcctctac cacaagtgat agctcagatt
    31741 catcaacttc aagcgatagt acagacacgt cgtctaccac aagcgataca cgtcatgcaa
    31801 gtgtctcctt ctcaaaaaca agttcggata acaggacgaa ctttaactta actaataaaa
    31861 caagcaatga caatggaaac aaatcaaaaa gagcacttcc taaaactgga agtcaatcga
    31921 ataattggat aacactcgca ggcgttatat tacttgtaat cggtttaaga atcactttta
    31981 tgagttataa gaaatcgaaa aggtaaaaaa atattcaaga taaaaaagag caacgtcgct
    32041 aacggacgtt gctctttatt tttgcttttt atcataaata caagaactac aattcaattt
    32101 gtcaaaaaac acgtagattt ccttctgaga aatttattta cgtatcgaac ctttccctat
    32161 tcagtctttg cgatccaaaa tcagttgggt aagggaaaag agattctctt ttgttcgaag
    32221 agaggtggca ggtaatttct ttatttcctt caaagccttt tctgtgtaag aggccgctaa
    32281 cttcctagct tcttccacgc ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc
    32341 ttcttctgtc attgccgctt tcttctctaa ataaggtagt agctttggtt tgtttttttg
    32401 caaagagtag atcaacggta aagaatagac cccttgacgc atgtcttcta gtacaggttt
    32461 gccgatttcc tggctctctt ttgtataatc taaaatatca tcgataatct gaaaggccat
    32521 cccgatagaa agtccgatat ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc
    32581 aaaagcgcca acagaacaac ttaattggaa cagttcagct gtcttacctg aaatattttc
    32641 caaatattct tttacagaaa gttcataatt atagcgatca tccatctgcc ctaattcgcc
    32701 attcaatact ttttccatac tgcgagaatt caattggaga cttttcaagg aagtggagta
    32761 atcagacaat agtttaaaac agcagacaaa taaatagtct cctgcataga cagaaacgac
    32821 attaccaaac tgactacgga tattaggctg accacgtcgc agatccgctt catcaacgat
    32881 atcatcatgg atcagtgtgg ccgtatggag catttcgata gaagctgcca aagcgatggc
    32941 tttgtctcga tcttgctctg gtccaaattg tgagaacaat agctgatagg caggacgcaa
    33001 cagctttccg ccggcatgga tcgtttcttt gatggcttgt tcgacaggtt tgtttttcag
    33061 gcggacagtt tcttccatcc gttttagtgt ttctgtcaat tctttactta aagcaggata
    33121 gttttcccac attggatgta gcttcataaa aaaactcctt gtgtgacgat tattttttgg
    33181 taaacattgg gctccatttt tgtttggttc cctgctcata atctttttgg aatcgttgaa
    33241 gtgtctgcac atgtcgtaaa agataattcg ctgcgatccc gattgcaatc cctgataaaa
    33301 tccctaaaac tgaaaggact ggcaagtaca acatgactgt ccatgattgt gcaatgaaag
    33361 aagccatcaa aagctgtccg acattatgca tgaaaccgcc ggccgcacta attccgatga
    33421 tactgacacg ttttggtcct aattgcttga ccaataacat tccaaaataa ctaagcaaag
    33481 caccactcat actataaagg aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata
    33541 gtcgtagcca aagtagtaag aagctgtctt gttttttcat ggtaaaaatg gcaacgatcg
    33601 tgattagatt ggctaagcct agttttgcac caggcgcaaa agaaaaagga taagggatca
    33661 tattttccaa taaaccaatg atcactcctt gtgcaacaag tagcgaaata taaatcattt
    33721 tttgtaattt actcatggtg tgtgaaggtc taataaatca gactgtcttc gtcacctcca
    33781 tctgttgaac ggacttcgat cactaattta tgtggcaaac agacgatggt ttcgccattt
    33841 ttcgttgccc agcctcgacg tacgcagatc tggtcatcgc aatctgcttc ttttatccgt
    33901 actcgatcgc catctatctc gatcaagtta taatctccgt gttcatcttt gtaagtatat
    33961 gtgttgctct cttctccagc catcaaaggg aatgttttga tttctgtgcc atccacacgc
    34021 agtaccgctt ctttttctgc tgttgcggtg ttttgctgaa gaccaaaaat catgactggt
    34081 aaaaaagaaa gaacagtcaa aagcaaaata ataaaaacat cccaaggttt gagcagactt
    34141 ttttcagcaa attctttaag gctcattttt gttctccttt ttccagatta actcacccaa
    34201 ttttagcata aaaacaagag gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa
    34261 gaatagaaaa cagctttcca tgttttctaa ttctttgggc ttattaccga gaagcagcag
    34321 gtggaacacc gtttataaca agaggttgtg acagaagtgg gcaacttcaa gaaataagaa
    34381 ggaatttacg aaaattactt ttcaagtttt tgtgaaattt cggc
//
LOCUS       DADOOS010000014        33736 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.014, whole genome shotgun sequence.
ACCESSION   DADOOS010000014 DADOOS010000000
VERSION     DADOOS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.014"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            214..498
                     /locus_tag="J8C37_000582"
     CDS             214..498
                     /locus_tag="J8C37_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAZ5304043.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="J8C37_000583"
     CDS             complement(779..1198)
                     /locus_tag="J8C37_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAZ5304044.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="J8C37_000584"
     CDS             complement(1200..1784)
                     /locus_tag="J8C37_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAZ5304045.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="J8C37_000585"
     CDS             complement(1831..3306)
                     /locus_tag="J8C37_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAZ5304046.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="J8C37_000586"
     CDS             3487..3798
                     /locus_tag="J8C37_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304047.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="J8C37_000587"
     CDS             complement(3913..6249)
                     /locus_tag="J8C37_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5304048.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="J8C37_000588"
     CDS             6391..7059
                     /locus_tag="J8C37_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304049.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="J8C37_000589"
     CDS             7062..8105
                     /locus_tag="J8C37_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5304050.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="J8C37_000590"
     CDS             complement(8342..9223)
                     /locus_tag="J8C37_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ5304051.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="J8C37_000591"
     CDS             9382..10110
                     /locus_tag="J8C37_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAZ5304052.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="J8C37_000592"
     CDS             10266..10583
                     /locus_tag="J8C37_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ5304053.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="J8C37_000593"
     CDS             10576..11184
                     /locus_tag="J8C37_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAZ5304054.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="J8C37_000594"
     CDS             11200..12030
                     /locus_tag="J8C37_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAZ5304055.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="J8C37_000595"
     CDS             12168..12680
                     /locus_tag="J8C37_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAZ5304056.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="J8C37_000596"
     CDS             12706..13578
                     /locus_tag="J8C37_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAZ5304057.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="J8C37_000597"
     CDS             13754..14116
                     /locus_tag="J8C37_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ5304058.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="J8C37_000598"
     CDS             14125..14877
                     /locus_tag="J8C37_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304059.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="J8C37_000599"
     CDS             14891..16693
                     /locus_tag="J8C37_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304060.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="J8C37_000600"
     CDS             16970..18010
                     /locus_tag="J8C37_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAZ5304061.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="J8C37_000601"
     CDS             complement(18062..20206)
                     /locus_tag="J8C37_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ5304062.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="J8C37_000602"
     CDS             complement(20563..21936)
                     /locus_tag="J8C37_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5304063.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="J8C37_000603"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="J8C37_000603"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAZ5304064.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="J8C37_000604"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="J8C37_000604"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAZ5304065.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="J8C37_000605"
     CDS             complement(23384..24397)
                     /locus_tag="J8C37_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5304066.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="J8C37_000606"
     CDS             complement(24478..25077)
                     /locus_tag="J8C37_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ5304067.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="J8C37_000607"
     CDS             25263..25964
                     /locus_tag="J8C37_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304068.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="J8C37_000608"
     CDS             25974..28685
                     /locus_tag="J8C37_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAZ5304069.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="J8C37_000609"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="J8C37_000609"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAZ5304070.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="J8C37_000610"
     CDS             complement(30770..31951)
                     /locus_tag="J8C37_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ5304071.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="J8C37_000611"
     CDS             32203..33726
                     /locus_tag="J8C37_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAZ5304072.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10690 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaa
//
LOCUS       DADOOS010000015        32094 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.015, whole genome shotgun sequence.
ACCESSION   DADOOS010000015 DADOOS010000000
VERSION     DADOOS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.015"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(24..203)
                     /locus_tag="J8C37_000612"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="J8C37_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="J8C37_000613"
     CDS             complement(407..766)
                     /locus_tag="J8C37_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAZ5304073.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="J8C37_000614"
     CDS             complement(796..1137)
                     /locus_tag="J8C37_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304074.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="J8C37_000615"
     CDS             complement(1134..1808)
                     /locus_tag="J8C37_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ5304075.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="J8C37_000616"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="J8C37_000616"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAZ5304076.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="J8C37_000617"
     CDS             complement(4322..5512)
                     /locus_tag="J8C37_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ5304077.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="J8C37_000618"
     CDS             complement(5533..6060)
                     /locus_tag="J8C37_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAZ5304078.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="J8C37_000619"
     CDS             complement(6107..8896)
                     /locus_tag="J8C37_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAZ5304079.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="J8C37_000620"
     CDS             complement(9043..9243)
                     /locus_tag="J8C37_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5304080.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="J8C37_000621"
     CDS             complement(9696..10016)
                     /locus_tag="J8C37_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304081.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10204)
                     /locus_tag="J8C37_000622"
     CDS             complement(10061..10204)
                     /locus_tag="J8C37_000622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304082.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(10519..11637)
                     /locus_tag="J8C37_000623"
     CDS             complement(10519..11637)
                     /locus_tag="J8C37_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304083.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="J8C37_000624"
     CDS             12108..13415
                     /locus_tag="J8C37_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAZ5304084.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="J8C37_000625"
     CDS             13536..14735
                     /locus_tag="J8C37_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAZ5304085.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="J8C37_000626"
     CDS             complement(14762..16030)
                     /locus_tag="J8C37_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAZ5304086.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="J8C37_000627"
     CDS             complement(16200..16841)
                     /locus_tag="J8C37_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAZ5304087.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="J8C37_000628"
     CDS             complement(17250..17753)
                     /locus_tag="J8C37_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ5304088.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="J8C37_000629"
     CDS             complement(17876..18640)
                     /locus_tag="J8C37_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAZ5304089.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="J8C37_000630"
     CDS             18748..19023
                     /locus_tag="J8C37_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAZ5304090.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="J8C37_000631"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="J8C37_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAZ5304091.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="J8C37_000632"
     CDS             complement(20579..20980)
                     /locus_tag="J8C37_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ5304092.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="J8C37_000633"
     CDS             complement(20994..21803)
                     /locus_tag="J8C37_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5304093.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="J8C37_000634"
     CDS             complement(21790..22680)
                     /locus_tag="J8C37_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5304094.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="J8C37_000635"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="J8C37_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="J8C37_000636"
     CDS             complement(23348..24289)
                     /locus_tag="J8C37_000636"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAZ5304095.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="J8C37_000637"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="J8C37_000637"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAZ5304096.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="J8C37_000638"
     CDS             complement(25274..26470)
                     /locus_tag="J8C37_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ5304097.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="J8C37_000639"
     CDS             complement(26488..27216)
                     /locus_tag="J8C37_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5304098.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="J8C37_000640"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="J8C37_000640"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAZ5304099.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="J8C37_000641"
     CDS             complement(28628..29086)
                     /locus_tag="J8C37_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAZ5304100.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="J8C37_000642"
     CDS             complement(29083..30546)
                     /locus_tag="J8C37_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5304101.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="J8C37_000643"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="J8C37_000643"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAZ5304102.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32094
                     /locus_tag="J8C37_000644"
     CDS             31602..>32094
                     /locus_tag="J8C37_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ5304103.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDS"
BASE COUNT         9354 a         6912 c         5503 g        10325 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atacgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcg
//
LOCUS       DADOOS010000016        29225 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.016, whole genome shotgun sequence.
ACCESSION   DADOOS010000016 DADOOS010000000
VERSION     DADOOS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.016"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..829)
                     /locus_tag="J8C37_000645"
     CDS             complement(<1..829)
                     /locus_tag="J8C37_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ5304104.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="J8C37_000646"
     CDS             complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="J8C37_000646"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAZ5304105.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3330..3509)
                     /locus_tag="J8C37_000647"
     CDS             complement(3330..3509)
                     /locus_tag="J8C37_000647"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAZ5304106.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3556..5379)
                     /gene="cadA"
                     /locus_tag="J8C37_000648"
     CDS             complement(3556..5379)
                     /gene="cadA"
                     /locus_tag="J8C37_000648"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAZ5304107.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5657..7936
                     /locus_tag="J8C37_000649"
     CDS             5657..7936
                     /locus_tag="J8C37_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5304108.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8029..8676)
                     /locus_tag="J8C37_000650"
     CDS             complement(8029..8676)
                     /locus_tag="J8C37_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304109.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8855..9574)
                     /locus_tag="J8C37_000651"
     CDS             complement(8855..9574)
                     /locus_tag="J8C37_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ5304110.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9705..10856)
                     /locus_tag="J8C37_000652"
     CDS             complement(9705..10856)
                     /locus_tag="J8C37_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAZ5304111.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11073..11813)
                     /locus_tag="J8C37_000653"
     CDS             complement(11073..11813)
                     /locus_tag="J8C37_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ5304112.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="J8C37_000654"
     CDS             complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="J8C37_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAZ5304113.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12197..12790)
                     /locus_tag="J8C37_000655"
     CDS             complement(12197..12790)
                     /locus_tag="J8C37_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ5304114.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12774..13421)
                     /locus_tag="J8C37_000656"
     CDS             complement(12774..13421)
                     /locus_tag="J8C37_000656"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAZ5304115.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="J8C37_000657"
     CDS             complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="J8C37_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAZ5304116.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="J8C37_000658"
     CDS             complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="J8C37_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAZ5304117.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14867..15394)
                     /locus_tag="J8C37_000659"
     CDS             complement(14867..15394)
                     /locus_tag="J8C37_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAZ5304118.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15574..16359)
                     /locus_tag="J8C37_000660"
     CDS             complement(15574..16359)
                     /locus_tag="J8C37_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAZ5304119.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16352..17221)
                     /locus_tag="J8C37_000661"
     CDS             complement(16352..17221)
                     /locus_tag="J8C37_000661"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAZ5304120.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17222..18601)
                     /locus_tag="J8C37_000662"
     CDS             complement(17222..18601)
                     /locus_tag="J8C37_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAZ5304121.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="J8C37_000663"
     CDS             complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="J8C37_000663"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAZ5304122.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19053..19529)
                     /gene="accB"
                     /locus_tag="J8C37_000664"
     CDS             complement(19053..19529)
                     /gene="accB"
                     /locus_tag="J8C37_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAZ5304123.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="J8C37_000665"
     CDS             complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="J8C37_000665"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAZ5304124.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="J8C37_000666"
     CDS             complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="J8C37_000666"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAZ5304125.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="J8C37_000667"
     CDS             complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="J8C37_000667"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAZ5304126.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22559..22783)
                     /locus_tag="J8C37_000668"
     CDS             complement(22559..22783)
                     /locus_tag="J8C37_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAZ5304127.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22836..23798)
                     /locus_tag="J8C37_000669"
     CDS             complement(22836..23798)
                     /locus_tag="J8C37_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAZ5304128.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23808..24248)
                     /locus_tag="J8C37_000670"
     CDS             complement(23808..24248)
                     /locus_tag="J8C37_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5304129.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24554..25519
                     /locus_tag="J8C37_000671"
     CDS             24554..25519
                     /locus_tag="J8C37_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAZ5304130.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25576..25806)
                     /locus_tag="J8C37_000672"
     CDS             complement(25576..25806)
                     /locus_tag="J8C37_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAZ5304131.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25892..26779)
                     /locus_tag="J8C37_000673"
     CDS             complement(25892..26779)
                     /locus_tag="J8C37_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAZ5304132.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26868..28091)
                     /locus_tag="J8C37_000674"
     CDS             complement(26868..28091)
                     /locus_tag="J8C37_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAZ5304133.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28108..29187)
                     /locus_tag="J8C37_000675"
     CDS             complement(28108..29187)
                     /locus_tag="J8C37_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAZ5304134.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8088 a         6411 c         5043 g         9683 t
ORIGIN      
        1 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
       61 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      121 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      181 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      241 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      301 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      361 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      421 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      481 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      541 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      601 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      661 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      721 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      781 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      841 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      901 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
      961 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1021 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1081 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1141 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1201 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1261 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1321 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1381 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1441 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1501 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1561 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1621 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1681 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1741 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1801 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1861 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1921 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     1981 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2041 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2101 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2161 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2221 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2281 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2341 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2401 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2461 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2521 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2581 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2641 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2701 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2761 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2821 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2881 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     2941 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3001 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3061 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3121 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3181 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3241 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3301 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3361 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3421 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3481 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3541 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3601 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3661 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3721 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3781 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3841 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3901 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     3961 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4021 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4081 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4141 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4201 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4261 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4321 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4381 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4441 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4501 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4561 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4621 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4681 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4741 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4801 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4861 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4921 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     4981 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5041 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5101 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5161 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5221 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5281 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5341 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5401 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5461 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5521 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5581 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5641 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5701 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5761 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5821 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5881 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     5941 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6001 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6061 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6121 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6181 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6241 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6301 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6361 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6421 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6481 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6541 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6601 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6661 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6721 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6781 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6841 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6901 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     6961 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7021 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7081 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7141 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7201 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7261 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7321 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7381 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7441 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7501 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7561 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7621 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7681 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7741 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7801 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7861 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7921 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     7981 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8041 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8101 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8161 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8221 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8281 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8341 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8401 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8461 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8521 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8581 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8641 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8701 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8761 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8821 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8881 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     8941 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9001 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9061 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9121 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9181 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9241 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9301 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9361 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9421 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9481 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9541 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9601 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9661 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9721 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9781 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9841 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9901 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     9961 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10021 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10081 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10141 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10201 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10261 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10321 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10381 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10441 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10501 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10561 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10621 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10681 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10741 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10801 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10861 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10921 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    10981 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11041 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11101 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11161 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11221 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11281 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11341 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11401 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11461 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11521 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11581 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11641 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11701 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11761 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11821 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11881 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    11941 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12001 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12061 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12121 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12181 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12241 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12301 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12361 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12421 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12481 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12541 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12601 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12661 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12721 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12781 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12841 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12901 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    12961 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13021 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13081 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13141 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13201 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13261 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13321 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13381 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13441 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13501 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13561 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13621 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13681 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13741 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13801 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13861 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13921 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    13981 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14041 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14101 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14161 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14221 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14281 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14341 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14401 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14461 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14521 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14581 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14641 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14701 gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14761 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14821 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14881 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    14941 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15001 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15061 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15121 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15181 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15241 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15301 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15361 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15421 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15481 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15541 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15601 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15661 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15721 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15781 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15841 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15901 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    15961 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16021 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16081 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16141 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16201 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16261 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16321 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16381 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16441 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16501 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16561 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16621 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16681 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16741 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16801 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16861 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16921 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    16981 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17041 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17101 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17161 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17221 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17281 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17341 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17401 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17461 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17521 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17581 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17641 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17701 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17761 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17821 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17881 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    17941 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18001 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18061 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18121 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18181 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18241 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18301 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18361 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18421 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18481 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18541 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18601 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18661 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18721 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18781 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18841 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18901 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    18961 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19021 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19081 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19141 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19201 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19261 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19321 attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc
    19381 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19441 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19501 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19561 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19621 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19681 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19741 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19801 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19861 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19921 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    19981 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20041 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20101 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20161 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20221 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20281 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20341 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20401 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20461 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20521 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20581 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20641 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20701 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20761 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20821 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20881 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    20941 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21001 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21061 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21121 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21181 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21241 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21301 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21361 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21421 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21481 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21541 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21601 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21661 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21721 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21781 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21841 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21901 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    21961 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22021 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22081 tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22141 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22201 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22261 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22321 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22381 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22441 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22501 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22561 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22621 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22681 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22741 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22801 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22861 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22921 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    22981 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23041 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23101 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23161 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23221 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23281 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23341 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23401 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23461 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23521 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23581 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23641 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23701 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23761 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23821 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23881 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    23941 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24001 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24061 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24121 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24181 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24241 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24301 atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta tatgagcttt
    24361 ttttaagatt ttactcattt aaaacaactg ataaatcagc atttataatt tcttttatta
    24421 gaaaaatcat ctatctcaca tattttgatt atcaaagtat tttctgtgaa cagaactata
    24481 gaattcccta aaacttgtcc attatttcaa aatttgttat agtcttttta ttcaaagcgt
    24541 aaaaggagat cctatgttaa aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta
    24601 tcttatgaaa gaccgagatg gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa
    24661 tagagtaagc agctattttc atcagaataa acagcattca aaaaaagtat tacgtatgat
    24721 tcatcatata acagattttg atttcgtcgt agtagacacg gaattagatg ctttattgct
    24781 cgagtgtcaa ttgatccaac attaccgtcc tttttataac cgacaaatga attatttcag
    24841 taattataat tatgtacata ttaccaataa gggatttgta ttaacagata ctcctacggc
    24901 tcgaacatac gggccttttc gtctctataa aaaaatgccg tctattttgc gtataatgga
    24961 agaaacttat caaatgcctt ggttgtctga aattagtttg ttagctttgc gagtccagct
    25021 tcctgaccta caggaaatga cttttgaaca gaaaaaaaag gaactccaag ggctgtttca
    25081 aggccgaaat aaaaagctac tgacttactt gaaaaaaaga caacaacatt tcatctatca
    25141 gttgaatttc gaaaaagccg gtatgcttca aaaagatatc gaattagtta cttattttat
    25201 tagacggatc caggaacaaa agcagttttt aagaacacca tcgctcacct tcagcatgcc
    25261 attagcagca gatgagagtc agaaaaagca ctacctcgtt tgttatggtc aacttgcaga
    25321 aacaatgatt acctctggtg acgtttctcc tgacttctat tatgaaaaga aagaagctca
    25381 tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag
    25441 ttaccgcaaa aaattagaaa aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc
    25501 tgaaaagcaa ctaaattagt tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt
    25561 caattatcct aaaatttatt ggattaagtc atagatttcc atcgctacga tatcgatatt
    25621 atcaaattcg tattgctgat gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt
    25681 atcataagtt actgtacact tctctacgcc ttctttttca aaacggcgaa cttgcagttc
    25741 attatctgct gactcgacca tagcctccag tcgttttatg attgcgacta attgtgaatg
    25801 tgtcatcgga ggtcctcctt ttcttatttc tgttatattg tatcaaaagc atagcctact
    25861 tgcattgatt ttctctaaaa aaactcgttt tttatgattt tttttctggt atttttcctt
    25921 caggccacca aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca
    25981 actcttcata tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg
    26041 cttcttctaa tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt
    26101 aatcttcttt taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac
    26161 ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca
    26221 tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct
    26281 gacctttccc agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca
    26341 gtacttgatc ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg
    26401 ctcctaagtc accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg
    26461 tttttgcaat tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc
    26521 ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg
    26581 ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg
    26641 cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag
    26701 ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc
    26761 cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc
    26821 tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat
    26881 gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct
    26941 tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta
    27001 gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa
    27061 atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa
    27121 ggatgatcga acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct
    27181 gccatcgctt ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa
    27241 tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca
    27301 aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt
    27361 cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc
    27421 aaggcttgga tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt
    27481 tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt
    27541 ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag
    27601 ctgcgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca
    27661 aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa
    27721 tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta
    27781 gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt
    27841 tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc
    27901 ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca
    27961 ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt
    28021 aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag
    28081 ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt
    28141 gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt
    28201 attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg
    28261 attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt
    28321 cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt
    28381 gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa
    28441 tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag
    28501 gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat
    28561 gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa
    28621 atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg
    28681 agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggt gatatttctc
    28741 taagtcgatc ccattggaaa tagcttgaat cggcacacgg ataccatagc tttccaacaa
    28801 ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag
    28861 atattttttt acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc
    28921 ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat
    28981 attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc
    29041 tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc
    29101 gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg
    29161 tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta
    29221 ttttt
//
LOCUS       DADOOS010000017        28400 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.017, whole genome shotgun sequence.
ACCESSION   DADOOS010000017 DADOOS010000000
VERSION     DADOOS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.017"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(218..1015)
                     /locus_tag="J8C37_000676"
     CDS             complement(218..1015)
                     /locus_tag="J8C37_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAZ5304135.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="J8C37_000677"
     CDS             complement(1012..2196)
                     /locus_tag="J8C37_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAZ5304136.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="J8C37_000678"
     CDS             complement(2221..3468)
                     /locus_tag="J8C37_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAZ5304137.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="J8C37_000679"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="J8C37_000679"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAZ5304138.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="J8C37_000680"
     CDS             5448..5762
                     /locus_tag="J8C37_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAZ5304139.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="J8C37_000681"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="J8C37_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAZ5304140.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="J8C37_000682"
     CDS             6447..7091
                     /locus_tag="J8C37_000682"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAZ5304141.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="J8C37_000683"
     CDS             7106..7435
                     /locus_tag="J8C37_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304142.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="J8C37_000684"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="J8C37_000684"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAZ5304143.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="J8C37_000685"
     CDS             8423..9247
                     /locus_tag="J8C37_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAZ5304144.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="J8C37_000686"
     CDS             9240..9587
                     /locus_tag="J8C37_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAZ5304145.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="J8C37_000687"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="J8C37_000687"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAZ5304146.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="J8C37_000688"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="J8C37_000688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAZ5304147.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="J8C37_000689"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="J8C37_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAZ5304148.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="J8C37_000690"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="J8C37_000690"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAZ5304149.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="J8C37_000691"
     CDS             15244..16011
                     /locus_tag="J8C37_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304150.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="J8C37_000692"
     CDS             16001..18043
                     /locus_tag="J8C37_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304151.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="J8C37_000693"
     CDS             18324..18473
                     /locus_tag="J8C37_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAZ5304152.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="J8C37_000694"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="J8C37_000694"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAZ5304153.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="J8C37_000695"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="J8C37_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAZ5304154.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="J8C37_000696"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="J8C37_000696"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAZ5304155.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="J8C37_000697"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="J8C37_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAZ5304156.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="J8C37_000698"
     CDS             22890..23972
                     /locus_tag="J8C37_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAZ5304157.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="J8C37_000699"
     CDS             23972..24775
                     /locus_tag="J8C37_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAZ5304158.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="J8C37_000700"
     CDS             24845..25585
                     /locus_tag="J8C37_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAZ5304159.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="J8C37_000701"
     CDS             25596..26600
                     /locus_tag="J8C37_000701"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAZ5304160.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28342)
                     /locus_tag="J8C37_000702"
     CDS             complement(26645..28342)
                     /locus_tag="J8C37_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304161.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         8945 a         5029 c         5854 g         8572 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa
//
LOCUS       DADOOS010000018        28370 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.018, whole genome shotgun sequence.
ACCESSION   DADOOS010000018 DADOOS010000000
VERSION     DADOOS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.018"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="J8C37_000703"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="J8C37_000703"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAZ5304162.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="J8C37_000704"
     CDS             complement(937..1518)
                     /locus_tag="J8C37_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5304163.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="J8C37_000705"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="J8C37_000705"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAZ5304164.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="J8C37_000706"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="J8C37_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAZ5304165.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="J8C37_000707"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="J8C37_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAZ5304166.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="J8C37_000708"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="J8C37_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAZ5304167.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="J8C37_000709"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="J8C37_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAZ5304168.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="J8C37_000710"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="J8C37_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAZ5304169.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="J8C37_000711"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="J8C37_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAZ5304170.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="J8C37_000712"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="J8C37_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAZ5304171.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="J8C37_000713"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="J8C37_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAZ5304172.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="J8C37_000714"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="J8C37_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAZ5304173.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="J8C37_000715"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="J8C37_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAZ5304174.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="J8C37_000716"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="J8C37_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAZ5304175.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="J8C37_000717"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="J8C37_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAZ5304176.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="J8C37_000718"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="J8C37_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAZ5304177.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="J8C37_000719"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="J8C37_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAZ5304178.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="J8C37_000720"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="J8C37_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAZ5304179.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="J8C37_000721"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="J8C37_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAZ5304180.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="J8C37_000722"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="J8C37_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAZ5304181.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="J8C37_000723"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="J8C37_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAZ5304182.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="J8C37_000724"
     CDS             13972..14157
                     /locus_tag="J8C37_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAZ5304183.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="J8C37_000725"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="J8C37_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAZ5304184.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="J8C37_000726"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="J8C37_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAZ5304185.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="J8C37_000727"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="J8C37_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAZ5304186.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="J8C37_000728"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="J8C37_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAZ5304187.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="J8C37_000729"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="J8C37_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAZ5304188.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="J8C37_000730"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="J8C37_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAZ5304189.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="J8C37_000731"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="J8C37_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAZ5304190.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="J8C37_000732"
     CDS             18232..18879
                     /locus_tag="J8C37_000732"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAZ5304191.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="J8C37_000733"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="J8C37_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAZ5304192.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="J8C37_000734"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="J8C37_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAZ5304193.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="J8C37_000735"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="J8C37_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAZ5304194.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="J8C37_000736"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="J8C37_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAZ5304195.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="J8C37_000737"
     CDS             20317..21255
                     /locus_tag="J8C37_000737"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAZ5304196.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="J8C37_000738"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="J8C37_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAZ5304197.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="J8C37_000739"
     CDS             21935..22774
                     /locus_tag="J8C37_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ5304198.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="J8C37_000740"
     CDS             22750..23619
                     /locus_tag="J8C37_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ5304199.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="J8C37_000741"
     CDS             23609..24406
                     /locus_tag="J8C37_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAZ5304200.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="J8C37_000742"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="J8C37_000742"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAZ5304201.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25416..25622)
                     /locus_tag="J8C37_000743"
     CDS             complement(25416..25622)
                     /locus_tag="J8C37_000743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304202.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(25688..28357)
                     /locus_tag="J8C37_000744"
     CDS             complement(25688..28357)
                     /locus_tag="J8C37_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ5304203.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     NVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9206 a         5280 c         6036 g         7848 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacatta agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac
//
LOCUS       DADOOS010000019        27783 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.019, whole genome shotgun sequence.
ACCESSION   DADOOS010000019 DADOOS010000000
VERSION     DADOOS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.019"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(283..1227)
                     /locus_tag="J8C37_000745"
     CDS             complement(283..1227)
                     /locus_tag="J8C37_000745"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAZ5304204.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1523..2467)
                     /locus_tag="J8C37_000746"
     CDS             complement(1523..2467)
                     /locus_tag="J8C37_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304205.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2688..3902
                     /locus_tag="J8C37_000747"
     CDS             2688..3902
                     /locus_tag="J8C37_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5304206.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3924..5939
                     /locus_tag="J8C37_000748"
     CDS             3924..5939
                     /locus_tag="J8C37_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAZ5304207.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6022..7395)
                     /locus_tag="J8C37_000749"
     CDS             complement(6022..7395)
                     /locus_tag="J8C37_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAZ5304208.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7421..7705)
                     /locus_tag="J8C37_000750"
     CDS             complement(7421..7705)
                     /locus_tag="J8C37_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5304209.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7707..9722)
                     /locus_tag="J8C37_000751"
     CDS             complement(7707..9722)
                     /locus_tag="J8C37_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ5304210.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9901..10206)
                     /locus_tag="J8C37_000752"
     CDS             complement(9901..10206)
                     /locus_tag="J8C37_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAZ5304211.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10295..11326)
                     /locus_tag="J8C37_000753"
     CDS             complement(10295..11326)
                     /locus_tag="J8C37_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAZ5304212.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11533..12408
                     /locus_tag="J8C37_000754"
     CDS             11533..12408
                     /locus_tag="J8C37_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ5304213.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12560..13402)
                     /locus_tag="J8C37_000755"
     CDS             complement(12560..13402)
                     /locus_tag="J8C37_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ5304214.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13494..15371)
                     /locus_tag="J8C37_000756"
     CDS             complement(13494..15371)
                     /locus_tag="J8C37_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ5304215.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15368..16789)
                     /locus_tag="J8C37_000757"
     CDS             complement(15368..16789)
                     /locus_tag="J8C37_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ5304216.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16792..20181)
                     /locus_tag="J8C37_000758"
     CDS             complement(16792..20181)
                     /locus_tag="J8C37_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAZ5304217.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20438..21829
                     /locus_tag="J8C37_000759"
     CDS             20438..21829
                     /locus_tag="J8C37_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ5304218.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21902..22549)
                     /locus_tag="J8C37_000760"
     CDS             complement(21902..22549)
                     /locus_tag="J8C37_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5304219.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22557..23243)
                     /locus_tag="J8C37_000761"
     CDS             complement(22557..23243)
                     /locus_tag="J8C37_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304220.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23249..24322)
                     /locus_tag="J8C37_000762"
     CDS             complement(23249..24322)
                     /locus_tag="J8C37_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAZ5304221.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24544..25218
                     /locus_tag="J8C37_000763"
     CDS             24544..25218
                     /locus_tag="J8C37_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304222.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25223..26563
                     /locus_tag="J8C37_000764"
     CDS             25223..26563
                     /locus_tag="J8C37_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5304223.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26712..27698)
                     /locus_tag="J8C37_000765"
     CDS             complement(26712..27698)
                     /locus_tag="J8C37_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAZ5304224.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8098 a         5251 c         5043 g         9391 t
ORIGIN      
        1 ctttattcag ttaatcaaat atgaacgaat tagatatgaa tcattgtatt tgccgttgcg
       61 gaaggatttt ttaattaacc acagataaac attgataacg attcgcttat tgtaatcaac
      121 agattaaatt tcatatgttc atatgtacgg atacattttc ggaccgatta attttaacca
      181 ttctgtttat ttgcttacct attagatatc aataaaaaac tgcttaacag ctatttcagc
      241 tgttaagcag ttttaggatt tttagttcat attcattgtt tcttataata cagtgttcaa
      301 tacttctctt aaagcacttg ttgatttgct gaacattgct ttttcttctt cgttgattga
      361 taattcaaca acttcacgaa caccattttc gtcaacgatt gatggcacac ctgtaaagat
      421 atctttttcg tcgtattcac cagttaaata agcagataca ggcaatacag cttgttcgtt
      481 attcaagata gcttttacga tacgtgcagt actcatacca ataccatagt aagttgcttt
      541 tttacgatca atgatctcat aagcagcatt acggacttta tcagcaatca catctaattc
      601 atcttgggca atacgatggt ctttttctac aatttcaaac actggtttac caccgacagt
      661 tgtatgtgac catgcagcaa cttctgaatc accatgttca cctaagatat agccgtggac
      721 gctgcgtggg tcaactttta atttcaatgc gatttcttta cggaaacgag ttgtatccaa
      781 tgttgttcca gtaccgataa cacgtgatgt tggcagacca gattcgttcc aagcgatata
      841 tgtcaaaata tctactgggt ttgaagcaac aacgataata ccgtcgaatc ctgatcccat
      901 gatttctttt acgatttggc gcatgattga cgcatttgtt ttaactaaat ctaaacgaga
      961 ttgaccaggt ttttggttga taccagctgt gataacaacg atatcagcat ctttacattc
     1021 ttcatagcca cctgaccata cagccacatt ttcatcgccc caagccatac catctaaaag
     1081 gtccaatgct tccccttctg ctttttcttg attcacatcg atcaatacta attcattaga
     1141 gattccttgg ttgatcattg cataagcgat acttgttcca acaaaacctg taccgacgat
     1201 tactacttta cgacttgttt ttttcatgaa taaacagctc cttttcataa ctatttgtta
     1261 ctttcatgaa aagtatagtc ttgtttattc attttatcaa catgttaatt ataatagttg
     1321 ttattataat ttttgatgga aacgaattcc tgaatacgca taacagataa atgacaaatg
     1381 tttacaccta tatcccctga taatacaacc aaaagtttag aactgaaaat aaatccaaaa
     1441 aaattcgctg caacgatata ataagacagc aatcgcacct aaaacaattt tccttcatat
     1501 gctttagtga aagattttct tcttaatgtt tatttctcca ttcattggga gtacatgttg
     1561 aatacttctt gaataatttg aataaagaag tcgtttgttt atatccgata cgattagcga
     1621 tttcttccaa cgaaaaactc gtttcttgaa tcagttccaa cgcaaagtcg aattttttct
     1681 ttagaaccag ttcttgaaag gaagcatcaa aatacatttt caatttatta ctaagataga
     1741 ccggattgta gccaaaatga gctgctgttt cttttaacgt acaagaagta aaatgcttat
     1801 cgatataatc taatactcta agcatctcat tttctgcgca tccatctgct gatgaaaagc
     1861 tttgttgttc taataaatga gtcaattcga tcagtaatag tgatgtatag agatttaacg
     1921 aacggatctt atgactgtat gtatcaaaat aaatcaacag catattttca atcaaatgcg
     1981 tcaaacgact tgtttttccg ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt
     2041 ttgatgcatt tatcaaaaaa gaagaaagta gattatttgt gcttgccaaa tgattcaata
     2101 tctctgtaga taaggtttct tcttttaata agatgttgat taaaatatcc tcttgccctg
     2161 tgtattcgat tctttgagta gcttctttat caatgatcac cacgtcactt tcttccatct
     2221 ctatcctttc tccattaata atttgtaaac atgtaccgct atacatataa ttgatttcaa
     2281 caaaatcatg tgtatgctcg ggaacttccg catatcgacg gtgtttactg atatgaatat
     2341 ttccctgttg aaatagatcc cctcggtgga tttttggcgg tgtctgaaga tggtaagcta
     2401 agtttaaatc attgatattc attccttgtt cacgctgcag agtttcgatt gattcttcgt
     2461 tacgcaaaaa aaatgtctaa ttcagctagt tccaattgac tccctccttt ttgtaagcgg
     2521 tttctttcgc ttattagttc acgctgtact gctattatat gcaaagctat ttttggtttc
     2581 ataaagactt tctatggttg ggacatgaac ttcctgtgat tccgctattg agagaataac
     2641 cacttcatac tatatttttg ataagcgctt acaaaaatga aaggaacatg cttatgaata
     2701 aaaagatgat gaaattttct ctgctgtcag tctctcttat tttgacttct gctggtgcag
     2761 tcacgtctgc aattcccagt atgtcagcta cttatgccac gcgttcttta tctgctgttg
     2821 aattattaac gactgctccc tcttttatgg tcactatttt tgttttgatt agtagtttta
     2881 ttgctaggaa aatcggacaa aaacaaactg tgatcatcgg attagtgatt gcatcgattt
     2941 gtggtatcct ccccctcttc tcacaaagtt acagtattgt cttagcttca agagctggac
     3001 taggtattgg ctttggattg atcaattctt tggcagtcag catgattgct gccttttatc
     3061 acggagatga acgagcctct atgattggtt ttcaaagtgc atttcaagga tttggtgcag
     3121 ctttaatgac atttgttgca ggacagctag tacagttcgg ttggcaatat acctttttag
     3181 tctacgccat cagcttacct attcttgtct tgtttgtttt gtttgttcct acacctgaaa
     3241 caactgaact tcaatcgcat ggcacagccc caacaagaca agtaatcaaa acgaatccta
     3301 aaatgattag ttatggtatt tttctcttgc tggtcatcgt ggtatataat gctgtatcga
     3361 ttaaattagc cacagtcctg acgacttatt ctctaggaaa cgaaactaat gcagcgaata
     3421 tcttaacaat catgcaattg gcaagtatgg ctgccggagt ttgttttggc tggcttcaga
     3481 caaaagcaca tcgctatatg cttacacttt ctttactact gatgggaatc ggctttgtat
     3541 tgattgccac tgtgataaac ctgtatgtga ttgggtttgg tgcgatccta acaggaattt
     3601 ctttttcgtt attcattcct tacttattca accaagtaag tatccaagcg cccaagtacg
     3661 cggagagttt caatacttca atcttattgg ttggcgctaa tttaggtact ttcattgcac
     3721 catatggttt gctctttctc tcttttgctt ctcttgggag taaaacactt gctgttttcg
     3781 tcaatggcag tatcttgctg ttgattagcg cgcttattag ccttctagta gtacacagga
     3841 cgaatagaaa gacagtgata agagtagatg aagaaataaa agaacaattg acaacaaaat
     3901 aaatttgatg gaggtcgtgt aaaatgacaa atgaaaaaca taaagataga tggctttggt
     3961 atcctggaga ctttgagatc cgccatggac tgctacaaaa tttccaacgg gaagaacgag
     4021 gttttgactg gccagcgtac tggtacatgg atgattgcca tcggaacgtc aagtttaaga
     4081 gatattactt tcttgatcaa ccttccatgt ttaaagtgac gatccaagga gttgggtatg
     4141 tagaaattaa tggacaaaaa catccatgtg gaaaatggct gacttgtcca gctggtaaag
     4201 caaaaattcg tatctttgta ggacatacat cagggctacc agcgatgttt atcgagggag
     4261 acgaagtaaa atcagatatt ggctggacag caagtaattt tattgaagaa tatccagctg
     4321 gctggagtcc gctttatgtc gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag
     4381 aatgcgtcca tccagtcact gaaacggaga ttggcggagg aatcttattc gattttgggc
     4441 gagcagtgaa tggctgtgta gaactaatat ctctggatga ccatatcgaa tctatcactc
     4501 tatgttatgg agagtccgat gtagaagcta tggatatcga ttattgctac tataagcaaa
     4561 taggaatgac aagaaaaatg aaagctcgta aacgcgcttt tcgctatttg tttataccag
     4621 aggtttctac tggaatggtt caacttcaag tttttcatga atttttatct ctttcgaaac
     4681 aagctgcttt ttcaagcgat tcttctttac tgaatcagat ctggtcagta tcagaagaag
     4741 ccttcaagtt atgtagtgat ttgtttttta tcgacggtat caaacgagat cgctggattt
     4801 ggtctggaga tgcgtatcaa tcattcatga tcaatcaata tctttttttt gacgaggaaa
     4861 tcaataaacg aacgatatta gcattaagag gacaaaatga aatcaagcag catatgaata
     4921 ctatcgttga ctattcgatc ttgtggttga ttggtatcga aaaccactat atgatgacga
     4981 aagactatga atttttacgc atcgtctacc ctaagatgca atcattaatg agatacttga
     5041 tggagcaaac gaatgaatta gggtttattt atgggagagc aaaagattgg atatttattg
     5101 attggtctga aatggataaa gaaggaacgc ttgctgcaga acaagtctta ttactgaaag
     5161 cctatcattc catgatcact tgcggagata tcttagctga aacatatgga gagaacgaaa
     5221 attatcatac tcatctctat caagaaaaat atcaactatt gaaacaaaac ctatttgact
     5281 acttttggga tgaagaactt ggcgctttta tcgattgcta tgaatctggg agaagaaatg
     5341 tgacgcgtca tgcgaatatt ttcgctgttt tattcgattt tgctaacaaa gaccaacagc
     5401 aatcgatctt aaagaatgtc ttattgaatg acactatcac acagattact actccatatt
     5461 tcaaattcta tgaacaagat gctttatgca aactcggaca gacgaatatc gtctaccaaa
     5521 cgattcttga ttattgggga gggatgttat ctcatgatgc ggtaactttc tgggaagaat
     5581 acgaccctac tcaaacagga aatgaaaagt acagtatgta tggagatcct tatggaaaaa
     5641 gcttatgcca tgcatgggga gcaagcccaa tctatctttt aggacgctat tttatcggtt
     5701 tgtatccaac aaagccaggt tatgaagaat ttatgataca acctaatcta gattcatttg
     5761 aaacactgaa ttgtacgcta cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag
     5821 ataagatcgt gattacttct gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt
     5881 atcctcttct agcaaaacag ccacttgtct taacaagcta ttcaaaaata aatgcataaa
     5941 acaaaaaact gcttgcgaca tatcagaaga ctttaaatct tcttctgata ttgtccaagc
     6001 aatttttatt ttgaagtttg cttaattctg ttctttagaa acaatgtttg ttctttttcc
     6061 ggcaaaaaga aagctagata atacgacaag taaaaagaaa gcgatgattc caactgtcaa
     6121 ggctatttga ccgtattctc ctagtttccc aagtacaagt cctggtacta gataatcagt
     6181 atcggcaaat gtagaagaag cgattttaag atctcctaat actggaagga taaataatgg
     6241 cagccaacta atcaataagc tattgaaagc agaacctaat accgcccctc ttctaccgcc
     6301 agtcgagttg ccgaagacac cagaagcagc tccgcagaag aaatgtccta ctaccccagg
     6361 aatgataact gtagtttgca agcctatcat agcaaacatg gatagtgttc ctacaaagaa
     6421 gctaacaaaa aagccaatta atacagcatt aggtgcatat ggaaaaacga taggaacatc
     6481 taatgctggt ttagaatcgg gtacgagttt tttagcaatc ccttggaatg ctggaacgat
     6541 ttctgccaac accattcgaa ccccttgaag gacgataaca aacccagctg tgaatgttcc
     6601 agcttggatc agtgcaaaga ttacagcgtt gtctccattg cttagttctc tttcaacaaa
     6661 tccaggacca gctaacaaag caagtgcgac atatacgatc gacatcaaga gcatgatcga
     6721 tacagtagaa tctcgcaaaa agctgaaact tttaggaatc tttatgtcct ctgtagaacg
     6781 gtctttgttt ccaaaaaact ttccgatcca tccactagaa gcataaccga tatttccggt
     6841 atgccctaac gcaacttgat tgtttccagt gatgttcttc ataaacggtt gacaaagagc
     6901 aggcgtaata gttaatagtg ttccttcgaa gaccccgcct aataagatca tccacaaatt
     6961 tgcagtaaac cccgcgctta ctaaaatgac tgctgttaga caagaaacat aaagcattgc
     7021 ttgacctgta agaaaaatga atttatatct tgtgaatcta gcaagtaaaa tattcacgat
     7081 catccctact agcatgatca aagcagtagg tgtaccgtat tgttgcaaag caatcgccac
     7141 tactgcttca ttgctaggaa ctactccttg agtatgcaga gctgtttgga acatcgaagc
     7201 aaaaggggtt agtgaattac tgattaaacc agcaccgccg atcagtacta ggaatccgac
     7261 aaatgttttg atagttcctt ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc
     7321 aagcagtgca atcaaagaaa cgaggatgga gggctgactg acgattgaca caaacaaatt
     7381 caacaattta tccattgata gttcctccct tgaatcattt ttattctttt gacaagatct
     7441 tcgttacttt ttcttttact tcttcgctgt caatgatcga ttgcaaaggt acgagctttt
     7501 catcaggtat agaagttagc gtatctttca aagaatgctc aatgaagaaa taatcagcca
     7561 tatcgctaga tacacttcca acatccgtat gacttacttc aaactcgtcc acatctgctc
     7621 caagatctgc aagtgcttgt ttgatgttca tctctaccat aaaacttgtt cctaatccac
     7681 tttgacatac tgcaaaaatt ttcatatcat tcgtctcctt ttcttatgat cgctaatatt
     7741 tcttcttttg ttgatgcgtg taataaatct tgaagatttt gtttattaga aagtagcttt
     7801 gttaaatttg ccaaagcccg aagatgtgcc tcattatcga tagcagctaa acagatgaac
     7861 aaactaatgg gatgggctgg attatcaagc agatccacag gttgttgaag tttcagcaaa
     7921 gacatcccta cttcgtttac gccatcttct ggacgagcat gaggtaatgc gatgtgatcg
     7981 ccaatatgga taaacgcacc atactgctcc acgcgctcga tcatagcttt gatataactt
     8041 tcttctattt tattttctga taatagcggc tgtgcggcaa atgtgattgc ttcttgccag
     8101 ttttttggtt gatccgataa ttggatcatt tcttctgtta ataattcaga tagcatagga
     8161 cgttgatcct ccttcttttc taaaagtttg ttcattttac gatttaatac ttgatagagc
     8221 ttttcttcgt ctacgccttc ttttaattcg atataaggca gtagcgccga caacaattct
     8281 tgggcagaag gcattgaaaa tcctggtatc agccaatctc tttgtacttg attgatcagc
     8341 tgattttttt ccaagtcagt catgattgga tgcagaacat agagagactt tttcttatca
     8401 gtaagtgtga gaggtacagt agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt
     8461 aagcggtcag ctgaattagt ctctgtaaaa aagatcatcg ggaataattg ctgaagttca
     8521 ctttttaaga tcaatgaaga gctaatgcca cttggacaga cgatcacggc tcttattttt
     8581 ctatttcttt cttctgcgtc cgcttttctg atctctccgc caaatagtat agtgaaaaaa
     8641 ccaatctctt cttcagggat gctcttccct attcgtcttt ctaacggaag taatgctttt
     8701 cttgtcattt cgtagatgga agcatattga tgcttaattt gatcgatcat cacattaggt
     8761 aaataaaaag aataggaaat acgaaaatag gcagggacca agtgataaaa gacattcatc
     8821 aataattcac gaaacgaagc aaattctacg gccatcagga attccatgcg atggatgact
     8881 tctgaagaac attccagcaa aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc
     8941 gatgctaagc aaagagagaa aaatataatc tccgaatttt gatgttcgga atagccaagg
     9001 ccagtcaata cttgtcgact aatgtcagtg actgcaagag aagccaacag ttcagcatct
     9061 tttaccagtt ccatggatgt ttttccaatt cgaatggttg tcaaactcaa ataataagct
     9121 aattcatcca ttcgatttgg tgcgaatcga agatctgata gtgaaatcgt tttatctagc
     9181 aaatctctaa ctttggcgta ataatcaatg gaaagggttg cggttaaata cgtgagccca
     9241 aatcttccag caagtgactg catcaaacga gcagtgcgat tttttgcttc tcttcttaat
     9301 attgcttctg accctagcag cctaaaacca gttttcctag tatacgacag ctgacaatct
     9361 tccacttcta attcatttct tatctttttg atatctgaca ggactgtccc tttagaaacc
     9421 tgaagaaaat cttgaaaatg aaaaacagaa acagacgttg cagtcgaata aaccatcaaa
     9481 taaagaagat ctcttcgctc ttgttcggta tacacagctt caaatcgagt aatatccaaa
     9541 aatttttctg cccagtcgat tgctcggaat ttctttatac ggatacgggt atcatccaca
     9601 tattcggcaa aaggagttaa ttgtttgatc gcctcgcttg tatttacttc gatgatctct
     9661 gctgcttttt ctagactgat ttctcttggt gttttcagga gataactaat tttgtagttc
     9721 acagcgctgt tcacctccta cttatattct agtatggtag cgcttttaaa tcgaggggtg
     9781 tttggtacaa cttacagttg gatttttttt acgactgtac cgaacgattt ctatcgaatg
     9841 gatacagaaa aaaagcaggt gaccaaatgt ctcctgcttt tatgaattga tttcgtccga
     9901 tcaatgatga tcatgtttat tttttgaggt cagttcttca aatttttctt tgacatcttc
     9961 aaccgctttt tcgatatctt tttcaaagtg attgggctca ttaggctttg tttcctcttc
    10021 tacagaattt gaaccagata aagctgtttc tttattttct gtttcaccag agtttgtgcc
    10081 agctaatgat tcttctttgt cttctgtttc accagaatta gttcctgcta aagccacttc
    10141 ttcttctgtt ttaggggcca ttgaacctct taatcgtttg tgtttttcac cgtcataatt
    10201 tgtcatgttg gttcctccta gtagttctat actttttcat cgtacaataa gacagtttat
    10261 gcgtcaaaaa acaaacccga ttcttacaaa tatgtcatat ttttccttct gattcttgaa
    10321 tgcttttgat tccttcttcc aaaattttca tttgtaagat tgtttcttca tctttgatga
    10381 ctttttctgc gccattaact aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac
    10441 caacctcgga aattactttt tcttcatgat aactcccttg ttcatctata taggtcaata
    10501 caccataatg ttcgggcaaa tcgataccaa agtcttcatg atcaggcatg taaaacattt
    10561 tcagatgttc ttcttgtcga tcttttgttt gtttcacgaa catccctttt tttccgtaaa
    10621 cgacgaaact tggacgttct ttgatgcgga aataactgga ttttatcgaa attttcatat
    10681 ttccatagaa aaaatccaga tcgaagtaat cattcattcg tcctttccct aaaagttgac
    10741 gtacatcata gctgatatca tctggttcgc cgaaataaga aagaacttga tctactgtat
    10801 gacaagcatg tccatataaa tagctgttcg cagtagaaaa ttcatgcgta ttttctggta
    10861 cttccggtct gaaataatca taatgcattt ctacttctaa aatatcccct aattttccgc
    10921 tttcgattac tttttggact gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt
    10981 ggataaatag acctttttct tttgctaatg caaataactc ttctgcttcc tttgacgttt
    11041 ctgcaaaagg tttctctact agtacattct taccatgttc gagtgccatt tttccatatt
    11101 cataatgaaa ttgactaggt gtagtgatga caactaattg gatctcagga tcatcccaaa
    11161 gatcagacag ctgatcagta tattcgatgt cttcgaatgt tttccacact ggttcagatg
    11221 gggaaaaaat ttttttgata ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat
    11281 agcgatttgt actctttcca ttgccaagat aagcgattgt aagcatgagt ggtgcctcct
    11341 tttattgatg ttttcagtat atcctagaaa agcgcagaaa gtatttataa tagctattct
    11401 ggtaaaaaag ttcagaaaaa cacttgttat cattcggttt ggtgaaatta atgttcaaag
    11461 taaggataaa aatcaaaaag atagtagcgg aaccaagtgt ttatcagtaa gatagaattt
    11521 ggaggtcaga tcatgcttat tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca
    11581 gagaaacgga ttgtcgatta catccgtagg aatatcgaag ctgcagtcta catgacgatt
    11641 gaagaattag caaaagctac gtatacttcc cattcagcta tcattcgttt atgcaaaaaa
    11701 atgggattca gcggatttaa agaattccgt ttcgagctgg ggcgggaagt tcatcaattg
    11761 attgctcagt tcaatcaaac gattgatcct aattttcctt tttcaagtga agatagcact
    11821 cagcaaattg caaagaaaat ggcggattta tcgatccagt caataaaaaa agcacaattg
    11881 cagattgaac atgaagattt agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc
    11941 tttctttttg caaaaggtga ttctcaaatc acggcaagga aattccagaa taaaatggtc
    12001 aagttgaata agtttttgat catggctgaa gaatacagtg attcttcttg gaatgcagcc
    12061 aatttgacta gcgaggattg cgccattttt attagctaca gcggtcgcat ccatcattat
    12121 gaacgaataa tgacctattt gaagcatgta gaggccccta ccttgctgat taccggaaat
    12181 cagcattcag aaatggccaa acaggctgat atgtgtcttg ttatatcgca ggaggaatat
    12241 gattttgcta aagtagcgac tttctcctct caaattgcct tcgattacgt attaaatacg
    12301 ttattctcag tgatatattc tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa
    12361 caagaattga tgcaaaaagg ccttcttaaa gaatatcgga aaacttaaga aaagatcctc
    12421 atttagtaat aagaaagata gtgtttgata ggctctcttt gatatcatca taaaaacagg
    12481 ctgaagtttt ctttttcaaa cctcagcctg tttgtttacg gaacgattcg aatagatata
    12541 ttatagaaat gaggaatagc taacttctgt ttcttatttt gaacgattga cgaaaaagat
    12601 atataagaag gaggaaaatc agtatacatg cagctaacaa tccatacagg aaaaatcgtt
    12661 tgttctcgga tactttctgt acttcttttt gaacttcttt tgcatgataa ggaacccggt
    12721 gccctctgac aagtaagcga tgactgttga ccatataagg tgtacaagtt aggagtgtca
    12781 cgaaatcctg ccctttttcg attctcagat cttttgtatc agtcggttct accgtcttga
    12841 tctgatcgac ttgataagcg agtgtccgtc cattgatttc gatatagaaa gaatcaccta
    12901 aagtcagttt cggtaagtct gtgaacaact ttgcttgagg cagtcctcga tggctggaaa
    12961 taacggcatg tgtattttct ccccccgtag gataagacgt tccttccaat aatgaggcac
    13021 ctttttctag cagcacagta ttcgtttcat caaaaattgg caaatggaca ttgatctcag
    13081 ggatcgtcaa aataccgatc gtatgtttct caaaataggt tctatccttt ttcgcaggtt
    13141 cttttttctt agtaaaggga tctgcacctg gatttccacc ttcttttgct aattgctggt
    13201 ttttctttgt catgttttct tggattttag ccatgacttc ttcattttct ttgaccgctt
    13261 gttgctgata atgagagatg atctgctgat ccagataatt attcagcgcg tcgctaacaa
    13321 atggataagc tagtatccct attcctgaga gaagcaagaa aagcatcagc agatctagaa
    13381 gtttcctttt ctttggtttc atttgtgttc tccttgtgat gactgtttga taataaaaga
    13441 aaaaggagca tttgcgtaat ccgctgctcc tttttccaat tcttgcaaaa agactaaatc
    13501 tgactgtgct tgcgtctagc aaagtacagt ccagcaatca gtagaaggac tactcctgca
    13561 ccgatataca catagattcc cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt
    13621 ggtatttttt caggagaaat caactgtcct gctgttacat aagattgttc atcgatagta
    13681 aatgctacac ggtttgttaa tggaacatat ttttctggcg ctttcgtttc ttccagatag
    13741 tacgtgccat attttagacc tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc
    13801 gttgctgatt ctttcgccga tacccagctg acggcttttg tggatgggtc gatcgataaa
    13861 tatttcgctg tgtcactatc ttgatcacga acgacgaatt ctgctccagc aagtgtttgg
    13921 tctgatgtaa cgtcaccatc tactttaaca aatcgtttgc ccccagtaac gacatcgact
    13981 gacggtggtg tttgatcatt tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct
    14041 ttggttggat ctgctttttc gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca
    14101 ggagttaatg aaggaatata attcggatca actgaaaccg tgaatccgtc tgtttgctca
    14161 gtgacagaat aatttactgg gtcgatttct ttatttccat catataaggc ataagcatac
    14221 gttcctgaag aatcattatc aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat
    14281 gttgtgtact tgttttgcgt tccttcttta tcagcgatac ctaatgggat attgacagaa
    14341 atctcatatt gaattttttc accgattgcg acatcttttc cattcaattg aggtgttgtt
    14401 ttatcaactt tagacgtatc atttttgatg gtcttttcta ctggtgtttg gctatttgcc
    14461 aggatctcaa aaggcgtgat tgtttgcttt tcaatcattt ccgcattatc aggagctttt
    14521 acttcttcta aattatattt tccaacttct aaatgattaa cgatcaactg gcctttttca
    14581 atagatgcct cggcaaagtc attgttgccg atgtcataag aatgaccagt aatgaaatgt
    14641 ttggctttgg tttgatcagt tgtccaagtg tacaatccat ctgtgacact ttggatgtat
    14701 tttttgacgc ttggtgttcc ttcttcttta gaaataataa attctgctcc atttagtgct
    14761 tcgttttcgg cagtaccgat ttttgtaact ttcaacgttc catcattacc gactgtattt
    14821 ttagggtaga gatggatagt atctagttct tctgtcccgt atttataaga gccatctgct
    14881 tgtttgatca tctcatatac agggaaagct aaaaccatgt ttgcggcagc tgacactccg
    14941 tcttttggtt cttctttgat cgtgtagact gcatctttcc cattttgttt tttaggtaaa
    15001 gataaagtga catttccatc agcatctgtc gttcctgaag caactggtgt accaggagtc
    15061 aaagactgga ctgcttgttt tgctgcatcc acggacgctc ctttatctcg ttgcgcataa
    15121 aattcttgag tgacgttata aactgaaaat gaaatatcgg ctaatccttg gtattgatcg
    15181 aattcgctca tttctttccc gctgttttgg attaaaggat cgggtaaatc agtcattttc
    15241 tttttatgaa ggatgacttg agctgctgtc tcctctgcaa acactttttt tgcgccgaag
    15301 ctgtttgtga gtaatggtaa aataaggaaa aggactccta acataacgtt tattttttta
    15361 tgatttttca ttttttcact cctgttcttt ttttgataaa caaccagatt gcaaagcttg
    15421 cgaataccat accaacaacg cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa
    15481 atggacggct attttctttt ttattgacta ctttcaatat actgtccgcc tctttatcct
    15541 cagaaagaaa agttgaaatg gtaaacggaa tttctgtttc acttaaaata taaccagaag
    15601 gtgcttcgat ctcttttaat cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc
    15661 catttttatc ggaaatcagg tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt
    15721 tggctgtttg ctggaggtaa tttcctgctt tgtttttaat aaggaatact gcgccagcta
    15781 atcgtttatc tggtttattt gtctcgattt tgacaaattg cttactccct gtatatacag
    15841 agactttctt tgtactgaca ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct
    15901 tgttcgcctg tgctgtactg cttagctgca tctgataaga aatggtcagt ttttttccag
    15961 catatttttc caacgaaggg atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta
    16021 tgacaaagcc ttctgtcagc ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa
    16081 acgttaatgc ttcatccgca ctatctgaca attcgaactt ttgatagtca aggatattta
    16141 caggaattgt cgtatgcaag gaataagtga ttttttcacc aatcgtaaaa tcatttctag
    16201 gctcttctaa taccttctca aaaggtggat cagggtagtc gttttcttca tttttaggat
    16261 acaaatggat agttgaaagt ttttgattgt tttgtccatg tacaggaaga acaactacca
    16321 tattctctgc cttttctttg acgacttctg gtactttgga ttcaatgaaa agatacgctt
    16381 tatctctttt ttcttgatct ttagcggcca gagaaaaaga agcgatacca tcttcgttgt
    16441 taagagttgt agttgttgct tctgcggtaa acataccgga agacgcaccg ttttttgcga
    16501 tctctgcttg tgcttcttct accgttttgc ccttttcccg tagatggtaa aaagaatctg
    16561 tgacatcata aacttggaat gtgacaccat ttaatcctcg atacgtttgt aataaagctt
    16621 tttcttgtcc gtcatttgga tgatttttcg gcagttgccc gtttgggaaa agcaatttgt
    16681 gtaaagtaat ctgaaccatt tctgtttctg tttctgttgc tacctgagta aaagctggtt
    16741 taaatagtaa caagaagaga cacatactaa gccaaccaag ttttttcata tttagctgcc
    16801 ccctctttct tttttcaaaa aaagatacca aatagaaaac gcacaaccaa tcatccctac
    16861 tagatagatt cctaaacggc caattcctcc cgtttcaggt aatggtactt ttgcctgatt
    16921 cgtgatgtct aaagaaatct ggttgttttt ggctcctggt gacagaacag attcatgatc
    16981 ttgtccatcc acttgaattg acccatcttc gtgtataact atagtaactg gctcttttag
    17041 accttggtat ccttctggtg taaaagtttc ggtcagcgta taagttccag gttttaaatt
    17101 ttcgaataga aaggtatctg tttcttgtcc atcttttggc gattctaagt ccatttctgg
    17161 tccctgcagt ctgaattttg ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac
    17221 cgttagatca aacgctttta gtcgattttg atgagtgagc gtccatacat tatttgtcat
    17281 ctcggtattt tttcccgtgt aaatgatttt tccatattta tcgatttgga attcataatt
    17341 tcccggcgaa gtatcatatc cggcaggacc agctgtttca acgactcgat atttaccagg
    17401 tgtgagtgaa tcaaaactgg caagaccttt ttcattcgtt gtttggctgt caattggctg
    17461 ataagtacca tttgcttctt ttttctgtag ggaaaatgct gctcctttaa gatttatctc
    17521 tttgtccgtc ccttgcatcg catacttacg tactgctaca ggaacttcaa caaaaggatt
    17581 ttcaatagtc aactggatcg tatcatcgga agtagtgact gtttttccat caatggttac
    17641 cgtaccatca gaagcgatct tgatttccca agtagtcttt ttgcttaatc catgcccttc
    17701 tggagctttt gtttctgtca gcgtataggt catttctttt tgcaatttga cattttctgg
    17761 aagagaatag gttccgtcgc catgatctgt tagtaaagta tcaatagaat ctcctgttaa
    17821 tttgaaaaca gcgccaataa gatccttttc acctgtagag gaatttttcg ttattttcaa
    17881 gtttaacggt gtgaattgtt tagtattttt ccatgtcatt gcatctattt gttgagaatc
    17941 gtatcctggt acaggtaatt ctttgattgt ttggtaactg aatgcttgac cttgattatt
    18001 gtattgaggt agtgataata tctcttgata actttctccg ctatttgcag ataatttgtc
    18061 aatgtctgca cgttcccacg tatttgttgt atctttagct ggtttaatga ttcgaatata
    18121 tccgtttttc caagctgcag catttgtcgt atgttcccgt tgaatctcaa aagtaacttg
    18181 atctggacga tcagctagat tgttgtcaaa ttcttcccat aactttttga tgtgaagact
    18241 gactccggga gctttagcag atggtatacc gaattcagct aattcattgg tatcaatgtt
    18301 tggctggaaa gttgtcctgc cgttcatttg ataccagaaa tttggatgga agtcctcatt
    18361 ttctgtttgg attctcactt ggtaatggat ttggatttct tggtcttttc ctaaatagat
    18421 ctgattgctc ttgatggtat ttccttctat ggaaatagat ggagtgaccg ttgtaggact
    18481 tgtccccaca ctcttgactg aaagtgtacc aggctgataa atgaatggtt ctgcgattgg
    18541 atcatttatt tgtccattgc ttacagttgc tgagatctgt acagcttttt tggctaggta
    18601 ttcagagata tctgttgcat gatcagctga ttcatagtaa agatcgcctt tttcatctga
    18661 tgaaaccatt tgtcgcatac gagactctac ttctgctttt gagagaccag ctgccggatc
    18721 gctttgaagt tggatgccaa gaccatgtat ttcgattcct cgttctttga gtgccatcgc
    18781 ttctccgatg gttgcgataa acgtactgtc gattcttctg gatagattgt tttggtcagg
    18841 tgcatcatag attcttgaga ttagagaagt atttcccggc cgatcttgcg tattagaaaa
    18901 ctgagttccg taataattgc tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa
    18961 tgttggtaca ccatccgtca gcaaaacgat cactttttta tgtccatttg gaacggatag
    19021 catgcttcct gcatctctta gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg
    19081 tgtaatggat tttacttgat ttttcactga atcaaatgaa cccatctgta ctgcaccgtt
    19141 actgtagcta tatccttcgc ttgagtagcc gacatatccc atattgattt tgtctgtgat
    19201 accgctatct gctaaagtat cgacaaaacg atcgacacca atctttactt caccgatccg
    19261 attattgtcg ttcatacttc ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat
    19321 atctttttgt acatttccac gagcattcaa ataaacatca aacaatccgg gtgtacttgt
    19381 ttcctttgca tactttcgta tcgcatagtc tgcttgatta ccggttcctc catattcgat
    19441 atacgaatgg gtccgatcat taggatctcc attccaagaa tttataccat cccattgatt
    19501 tgttccgttt ttattgcctt gatgatcaag aacatttgta tttcctgtag gttgccaact
    19561 ggctttagga taagtgccct gatcatctgt tgtatattct gtaggaatcg tttgattaga
    19621 tggcacactc acagctggca aggataatgt aggcaatgta aatgggctga cagaattttc
    19681 gttttctttt gatgattctt cttctatttc gtctggcttt ggtactgaca catctgtatc
    19741 tttcttattt tctgtctcat caataatgtc atattcttta ttatcgacga tcagcttgtt
    19801 tgcctggctg tggtaaagtg ctggatcgat tggaaaactc agatcgatag tttgtttagt
    19861 gtttgatgag atttccaacg taattttccc gctatttacc tgataagcat agccatctcc
    19921 ttcttttctt tccacatcac ttaagtaatt ttcgactaga ccaatttctt tttctatctt
    19981 ttcagcactg gtgttattcg catttagctt gatgttcagt gctttttttc cagactgatc
    20041 cgaaatgact tcagctgaaa ttgtttctaa aactgttgga tcatctgtag tgtctgctat
    20101 tgcagataat ggcacaaagt ttgataaact caatattatc agcatgaaaa taactttcag
    20161 tttcttccct gtggtcgtca tttttcccct cctttttctt tttttctgtt ttgacgtgaa
    20221 tagtgaaaat tattaaaata aacaggcgtt acataatttt atttatttta atatgataat
    20281 tattatacga agggcgctaa agggaatcac accaattttc ataccaattg gtttcattta
    20341 tgaagttgat tccttcgatt aatgagcgtt tcattgaaag aaaagggttg agggagaaaa
    20401 acatatgatc aggttgttgg aatgaaacta tagaaagatg gaaaaaatca ttcgaattct
    20461 gtccatttct gacaagtgga tgactaaaaa agaacttgcc aaacgaattg gtagttcaga
    20521 atctacattt gtaaggtata tcgaagagat caaaatccgc tggggaacgg atgtaacgat
    20581 tcaatcttca caaaaattgg ggtatcgtct tgaacatttg aatgtaccag cttatttaaa
    20641 tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg agattattgt
    20701 caatccgggt aaaacgattg agtattattg tgaaaaaatt tccatcagcc ggtcttcttt
    20761 tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc tatttattaa aagtgatcgt
    20821 tgaccaaggt tttcagttaa ctagccttaa aagtgagctc cagttgcgta tctttgttac
    20881 ctttttcttt ttaagttact acggaagacg cacgttgcca tttccaatcg aaaaaaagaa
    20941 gatcaaagaa attatggtca agaataattg tcagatgaat ttgtttcagg aagaaagccc
    21001 atatgagcaa tctttcttta ttatgtattt tgttgtgcat ctcatgagag aatcccaagg
    21061 aactccattt gaatggaaag ctggagactc ttatgtacag ttgataaata tcgataaaaa
    21121 agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt
    21181 agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat
    21241 cagtgagcag atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga
    21301 aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt
    21361 cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca
    21421 ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact
    21481 gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc
    21541 tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg
    21601 acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc
    21661 tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg agtatgatct
    21721 gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag
    21781 tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa
    21841 acagctatgt ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt
    21901 tttattgtat tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt
    21961 cttttgtcca attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa
    22021 agtataggaa actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata
    22081 attccagcca tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct
    22141 gctgatcatt tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta
    22201 agtcgcagca ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc
    22261 taatgaattt taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat
    22321 aagcatcttc caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt
    22381 caacgatttc tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata
    22441 ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc
    22501 acatctctcc tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat
    22561 tccttctttt tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc
    22621 cattgaatca tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct
    22681 tctttaacta gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt
    22741 tcgtctgcta agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt
    22801 ctttctccac cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat
    22861 tgcagcaagg aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt
    22921 tcaaattgtt cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct
    22981 atttctttaa agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg
    23041 ttgatcacta tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta
    23101 cttttacctg aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa
    23161 tcaattgctt tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt
    23221 ttcatcgtta atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt
    23281 tggttatcgt acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac
    23341 tatatagcaa ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac
    23401 tagtcaaata agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact
    23461 gtgctactaa agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa
    23521 aaatagcagt cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca
    23581 ctaagaacaa aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa
    23641 tactttcaat gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg
    23701 aaattttagc tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa
    23761 acggctgttg tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag
    23821 aaactgtgta ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc
    23881 caattttgat cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga
    23941 tttcattctt tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt
    24001 tcgttgtgcc gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca
    24061 atccgattgg tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg
    24121 aagctgcaaa tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat
    24181 cgattgcttt tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga
    24241 catacgcaat caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa
    24301 tttctttgat agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa
    24361 tgtcacttgt tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc
    24421 ttatcaataa attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt
    24481 tatttaagcc ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg
    24541 aaaatgatta aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa
    24601 atactcgctg caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag
    24661 ttggaaagtg gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga
    24721 tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact
    24781 gcaaaagata gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta
    24841 acaaaacctt tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg
    24901 ctaggtctct ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc
    24961 aatgaagtct tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc
    25021 gtttattttt tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc
    25081 tggggatttg attctgatac gacgattaca gttattgaag tttatatgag caacttacga
    25141 aaacatctgc gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat
    25201 atactgcaaa gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt
    25261 ttttgcgaca aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt
    25321 acagatatta aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt
    25381 tgattctcga acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa
    25441 cccgactcaa acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa
    25501 tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc
    25561 agctattggt gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa
    25621 taccatccaa aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc
    25681 gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa
    25741 tacaaatcaa atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat
    25801 tgttttttgg attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc
    25861 tattattctt tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg
    25921 cacaccgtta acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac
    25981 tattattgaa gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg
    26041 attgacttcg gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa
    26101 gcaaatgatc aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc
    26161 catcatagat ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga
    26221 tgaaaaaaaa atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa
    26281 tccagaagat aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt
    26341 aaagaatact ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta
    26401 tcgggaagat caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc
    26461 aaaacaggta atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg
    26521 agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa
    26581 gggctgtgac aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa
    26641 gtagcttctt cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct
    26701 ttagcttacg cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca
    26761 atatccaatt cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct
    26821 ttatgaatcc caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct
    26881 ttgattggga tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga
    26941 tctgttactg gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt
    27001 tcagtatcgt tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta
    27061 tttacatcta atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga
    27121 tcgatgtctt cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga
    27181 tcagttcctg gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct
    27241 acgtccattg gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg
    27301 ctaacagtat cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt
    27361 actgtgatga caaattttcc atcagaaata tctcctctat attgatataa aggatcattt
    27421 tctggtgtag ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc
    27481 cataaagaac ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca
    27541 gacaatccgt caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca
    27601 tctcgttcat acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta
    27661 ctatagcttc cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaatag
    27721 atttatgatt ggtatattgt tacttataaa tttattctat aggagacgat tgttttctac
    27781 aaa
//
LOCUS       DADOOS010000020        27638 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.020, whole genome shotgun sequence.
ACCESSION   DADOOS010000020 DADOOS010000000
VERSION     DADOOS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.020"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(208..2100)
                     /locus_tag="J8C37_000766"
     CDS             complement(208..2100)
                     /locus_tag="J8C37_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304225.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2097..3824)
                     /locus_tag="J8C37_000767"
     CDS             complement(2097..3824)
                     /locus_tag="J8C37_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304226.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3840..4490)
                     /locus_tag="J8C37_000768"
     CDS             complement(3840..4490)
                     /locus_tag="J8C37_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5304227.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4694..4990
                     /locus_tag="J8C37_000769"
     CDS             4694..4990
                     /locus_tag="J8C37_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAZ5304228.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5162..5782
                     /locus_tag="J8C37_000770"
     CDS             5162..5782
                     /locus_tag="J8C37_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAZ5304229.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5874..7040)
                     /locus_tag="J8C37_000771"
     CDS             complement(5874..7040)
                     /locus_tag="J8C37_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ5304230.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7052..7405)
                     /locus_tag="J8C37_000772"
     CDS             complement(7052..7405)
                     /locus_tag="J8C37_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAZ5304231.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7533..8555
                     /locus_tag="J8C37_000773"
     CDS             7533..8555
                     /locus_tag="J8C37_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAZ5304232.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8994..9428)
                     /locus_tag="J8C37_000774"
     CDS             complement(8994..9428)
                     /locus_tag="J8C37_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304233.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9538..11397)
                     /locus_tag="J8C37_000775"
     CDS             complement(9538..11397)
                     /locus_tag="J8C37_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAZ5304234.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11515..12675)
                     /locus_tag="J8C37_000776"
     CDS             complement(11515..12675)
                     /locus_tag="J8C37_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAZ5304235.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12982..14406)
                     /gene="araA"
                     /locus_tag="J8C37_000777"
     CDS             complement(12982..14406)
                     /gene="araA"
                     /locus_tag="J8C37_000777"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAZ5304236.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14422..15120)
                     /locus_tag="J8C37_000778"
     CDS             complement(14422..15120)
                     /locus_tag="J8C37_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAZ5304237.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15077..16738)
                     /locus_tag="J8C37_000779"
     CDS             complement(15077..16738)
                     /locus_tag="J8C37_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAZ5304238.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16956..18356
                     /locus_tag="J8C37_000780"
     CDS             16956..18356
                     /locus_tag="J8C37_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAZ5304239.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18608..19687)
                     /locus_tag="J8C37_000781"
     CDS             complement(18608..19687)
                     /locus_tag="J8C37_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5304240.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19773..20732)
                     /locus_tag="J8C37_000782"
     CDS             complement(19773..20732)
                     /locus_tag="J8C37_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAZ5304241.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20744..22267)
                     /locus_tag="J8C37_000783"
     CDS             complement(20744..22267)
                     /locus_tag="J8C37_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAZ5304242.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22277..22840)
                     /locus_tag="J8C37_000784"
     CDS             complement(22277..22840)
                     /locus_tag="J8C37_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAZ5304243.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22846..23709)
                     /locus_tag="J8C37_000785"
     CDS             complement(22846..23709)
                     /locus_tag="J8C37_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304244.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23723..24619)
                     /locus_tag="J8C37_000786"
     CDS             complement(23723..24619)
                     /locus_tag="J8C37_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304245.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24794..26065)
                     /locus_tag="J8C37_000787"
     CDS             complement(24794..26065)
                     /locus_tag="J8C37_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304246.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26055..27572)
                     /locus_tag="J8C37_000788"
     CDS             complement(26055..27572)
                     /locus_tag="J8C37_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAZ5304247.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8071 a         5833 c         4779 g         8955 t
ORIGIN      
        1 ttttgaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
       61 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      121 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      181 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      241 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      301 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      361 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      421 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      481 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      541 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      601 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      661 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      721 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      781 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      841 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
      901 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
      961 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1021 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1081 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1141 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1201 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1261 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1321 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1381 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1441 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1501 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1561 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1621 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1681 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1741 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1801 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1861 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     1921 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     1981 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2041 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2101 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2161 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2221 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2281 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2341 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2401 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2461 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2521 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2581 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2641 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2701 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2761 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2821 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     2881 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     2941 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3001 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3061 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3121 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3181 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3241 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3301 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3361 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3421 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3481 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3541 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3601 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3661 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3721 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3781 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3841 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     3901 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     3961 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4021 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4081 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4141 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4201 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4261 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4321 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4381 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4441 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc
     4501 ctttctttca agatgacaca ttgtcatatg acgctgtgtc attataacac ctcctacctg
     4561 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4621 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4681 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4741 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4801 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4861 gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     4921 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     4981 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5041 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5101 atagcctttt ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca
     5161 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5221 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5281 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5341 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5401 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5461 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5521 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5581 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5641 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5701 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5761 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5821 aatcttcaaa gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct
     5881 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5941 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     6001 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     6061 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6121 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6181 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc
     6241 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6301 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6361 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6421 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6481 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6541 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6601 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6661 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6721 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6781 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6841 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6901 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6961 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     7021 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     7081 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7141 gacaagcatc aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg
     7201 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7261 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7321 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7381 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7441 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7501 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7561 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7621 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7681 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7741 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7801 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7861 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7921 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7981 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     8041 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     8101 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8161 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8221 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8281 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8341 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8401 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8461 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8521 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8581 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8641 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8701 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8761 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8821 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8881 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8941 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     9001 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     9061 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9121 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9181 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat
     9241 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9301 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9361 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9421 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9481 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9541 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9601 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9661 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9721 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9781 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9841 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9901 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9961 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    10021 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    10081 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10141 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10201 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10261 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10321 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10381 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10441 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10501 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10561 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10621 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10681 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10741 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10801 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10861 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10921 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10981 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    11041 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    11101 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11161 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11221 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11281 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11341 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11401 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11461 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11521 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11581 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11641 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11701 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11761 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11821 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11881 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11941 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    12001 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    12061 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12121 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12181 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12241 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12301 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12361 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12421 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12481 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12541 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12601 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12661 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12721 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12781 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12841 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12901 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12961 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    13021 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    13081 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13141 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13201 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13261 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13321 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13381 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13441 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13501 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13561 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13621 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13681 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13741 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13801 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13861 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13921 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13981 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    14041 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    14101 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14161 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14221 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14281 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14341 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14401 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14461 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14521 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14581 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14641 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14701 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14761 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14821 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14881 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14941 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    15001 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    15061 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15121 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15181 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15241 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15301 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15361 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15421 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15481 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15541 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15601 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15661 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15721 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15781 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15841 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15901 tgttccctgt ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15961 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    16021 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    16081 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16141 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16201 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16261 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16321 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16381 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16441 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16501 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16561 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16621 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16681 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16741 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16801 atgattattg tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa
    16861 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16921 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16981 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    17041 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    17101 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17161 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17221 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17281 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17341 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17401 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga
    17461 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17521 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17581 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17641 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17701 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17761 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17821 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17881 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17941 tgggactttc attcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    18001 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    18061 cactgacatg ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18121 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18181 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18241 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18301 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18361 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    18421 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    18481 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    18541 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    18601 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    18661 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    18721 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    18781 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    18841 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    18901 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    18961 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    19021 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    19081 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    19141 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    19201 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    19261 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    19321 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    19381 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    19441 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    19501 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    19561 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    19621 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    19681 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    19741 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    19801 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    19861 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    19921 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    19981 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    20041 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    20101 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    20161 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    20221 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    20281 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    20341 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    20401 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    20461 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    20521 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    20581 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    20641 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    20701 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    20761 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    20821 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    20881 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    20941 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    21001 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    21061 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    21121 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    21181 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    21241 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    21301 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    21361 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    21421 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    21481 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    21541 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    21601 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    21661 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    21721 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    21781 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    21841 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    21901 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    21961 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    22021 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    22081 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    22141 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    22201 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    22261 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    22321 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    22381 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    22441 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    22501 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    22561 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    22621 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    22681 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    22741 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    22801 caatcaactg tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc
    22861 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    22921 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    22981 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    23041 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    23101 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    23161 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    23221 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    23281 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    23341 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    23401 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    23461 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    23521 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    23581 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    23641 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    23701 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    23761 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    23821 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    23881 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    23941 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    24001 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    24061 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    24121 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    24181 agccgacttg tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa
    24241 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    24301 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    24361 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    24421 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    24481 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    24541 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    24601 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    24661 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    24721 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    24781 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    24841 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    24901 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    24961 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    25021 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    25081 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    25141 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    25201 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    25261 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    25321 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    25381 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    25441 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    25501 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    25561 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    25621 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    25681 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    25741 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    25801 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    25861 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    25921 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    25981 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    26041 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    26101 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    26161 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    26221 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    26281 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    26341 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    26401 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    26461 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg
    26521 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    26581 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    26641 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    26701 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    26761 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    26821 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    26881 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    26941 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    27001 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    27061 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    27121 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    27181 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    27241 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    27301 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    27361 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    27421 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    27481 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    27541 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    27601 taatacacct cgttgaaagc gttgtcaata atataaat
//
LOCUS       DADOOS010000021        27538 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.021, whole genome shotgun sequence.
ACCESSION   DADOOS010000021 DADOOS010000000
VERSION     DADOOS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.021"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            22..669
                     /locus_tag="J8C37_000789"
     CDS             22..669
                     /locus_tag="J8C37_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAZ5304248.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            753..1697
                     /locus_tag="J8C37_000790"
     CDS             753..1697
                     /locus_tag="J8C37_000790"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAZ5304249.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1832..3283
                     /locus_tag="J8C37_000791"
     CDS             1832..3283
                     /locus_tag="J8C37_000791"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAZ5304250.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3304..4140
                     /locus_tag="J8C37_000792"
     CDS             3304..4140
                     /locus_tag="J8C37_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAZ5304251.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4140..4799
                     /locus_tag="J8C37_000793"
     CDS             4140..4799
                     /locus_tag="J8C37_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ5304252.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4925..5614
                     /locus_tag="J8C37_000794"
     CDS             4925..5614
                     /locus_tag="J8C37_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304253.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5620..6576
                     /locus_tag="J8C37_000795"
     CDS             5620..6576
                     /locus_tag="J8C37_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAZ5304254.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6620..7132
                     /locus_tag="J8C37_000796"
     CDS             6620..7132
                     /locus_tag="J8C37_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5304255.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7388..7936)
                     /locus_tag="J8C37_000797"
     CDS             complement(7388..7936)
                     /locus_tag="J8C37_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5304256.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8025..9206)
                     /locus_tag="J8C37_000798"
     CDS             complement(8025..9206)
                     /locus_tag="J8C37_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAZ5304257.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9442..10950)
                     /locus_tag="J8C37_000799"
     CDS             complement(9442..10950)
                     /locus_tag="J8C37_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAZ5304258.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11109..11300
                     /locus_tag="J8C37_000800"
     CDS             11109..11300
                     /locus_tag="J8C37_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304259.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11503..12420
                     /locus_tag="J8C37_000801"
     CDS             11503..12420
                     /locus_tag="J8C37_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAZ5304260.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12413..13486
                     /locus_tag="J8C37_000802"
     CDS             12413..13486
                     /locus_tag="J8C37_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304261.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13634..14152
                     /locus_tag="J8C37_000803"
     CDS             13634..14152
                     /locus_tag="J8C37_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304262.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14133..14525
                     /locus_tag="J8C37_000804"
     CDS             14133..14525
                     /locus_tag="J8C37_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAZ5304263.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14601..15536
                     /locus_tag="J8C37_000805"
     CDS             14601..15536
                     /locus_tag="J8C37_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAZ5304264.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15669..16448
                     /locus_tag="J8C37_000806"
     CDS             15669..16448
                     /locus_tag="J8C37_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAZ5304265.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16441..16758
                     /locus_tag="J8C37_000807"
     CDS             16441..16758
                     /locus_tag="J8C37_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAZ5304266.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17002..18612
                     /locus_tag="J8C37_000808"
     CDS             17002..18612
                     /locus_tag="J8C37_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ5304267.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18769..19617
                     /locus_tag="J8C37_000809"
     CDS             18769..19617
                     /locus_tag="J8C37_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ5304268.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19848..20327
                     /locus_tag="J8C37_000810"
     CDS             19848..20327
                     /locus_tag="J8C37_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304269.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20360..22588
                     /locus_tag="J8C37_000811"
     CDS             20360..22588
                     /locus_tag="J8C37_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAZ5304270.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22717..24360
                     /locus_tag="J8C37_000812"
     CDS             22717..24360
                     /locus_tag="J8C37_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAZ5304271.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24433..24978)
                     /locus_tag="J8C37_000813"
     CDS             complement(24433..24978)
                     /locus_tag="J8C37_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAZ5304272.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25001..25486)
                     /locus_tag="J8C37_000814"
     CDS             complement(25001..25486)
                     /locus_tag="J8C37_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ5304273.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25476..26288)
                     /locus_tag="J8C37_000815"
     CDS             complement(25476..26288)
                     /locus_tag="J8C37_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAZ5304274.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26427..>27538)
                     /locus_tag="J8C37_000816"
     CDS             complement(26427..>27538)
                     /locus_tag="J8C37_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAZ5304275.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9020 a         5020 c         5517 g         7981 t
ORIGIN      
        1 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
       61 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
      121 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
      181 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
      241 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
      301 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
      361 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
      421 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
      481 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
      541 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
      601 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
      661 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
      721 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
      781 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
      841 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
      901 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
      961 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     1021 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     1081 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     1141 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     1201 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     1261 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     1321 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     1381 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     1441 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     1501 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     1561 tacagattgg taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     1621 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     1681 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     1741 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     1801 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     1861 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
     1921 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
     1981 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
     2041 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
     2101 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
     2161 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
     2221 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
     2281 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
     2341 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
     2401 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
     2461 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
     2521 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
     2581 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
     2641 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
     2701 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
     2761 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
     2821 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
     2881 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
     2941 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
     3001 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
     3061 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
     3121 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
     3181 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
     3241 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
     3301 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
     3361 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
     3421 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
     3481 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
     3541 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
     3601 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
     3661 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
     3721 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
     3781 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
     3841 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
     3901 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
     3961 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
     4021 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
     4081 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
     4141 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
     4201 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt
     4261 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
     4321 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt
     4381 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
     4441 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
     4501 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
     4561 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
     4621 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
     4681 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
     4741 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa
     4801 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
     4861 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
     4921 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
     4981 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
     5041 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
     5101 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
     5161 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
     5221 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
     5281 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
     5341 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
     5401 ttttccaaca gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
     5461 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
     5521 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
     5581 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
     5641 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
     5701 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
     5761 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
     5821 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
     5881 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
     5941 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
     6001 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
     6061 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
     6121 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
     6181 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
     6241 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
     6301 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
     6361 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
     6421 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
     6481 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
     6541 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
     6601 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
     6661 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
     6721 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
     6781 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc
     6841 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
     6901 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
     6961 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
     7021 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
     7081 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
     7141 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
     7201 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
     7261 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
     7321 tcgagaggtc gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc
     7381 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
     7441 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
     7501 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
     7561 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
     7621 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
     7681 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
     7741 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
     7801 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
     7861 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
     7921 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
     7981 aaatatttcc aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca
     8041 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
     8101 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
     8161 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
     8221 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
     8281 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
     8341 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
     8401 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
     8461 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
     8521 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
     8581 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
     8641 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
     8701 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
     8761 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
     8821 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
     8881 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
     8941 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
     9001 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
     9061 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
     9121 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
     9181 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
     9241 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
     9301 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
     9361 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
     9421 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
     9481 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
     9541 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
     9601 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
     9661 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
     9721 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
     9781 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
     9841 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
     9901 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
     9961 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    10021 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    10081 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    10141 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    10201 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    10261 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    10321 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    10381 cagttggttc aaggaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    10441 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    10501 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    10561 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    10621 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    10681 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    10741 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    10801 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact agcttcccac caaattctaa
    10861 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    10921 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    10981 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    11041 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    11101 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    11161 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    11221 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    11281 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    11341 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    11401 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    11461 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    11521 aatgcaaatt atgagccaga cgaactagag gagacgaatg cggatcagga tacaggacaa
    11581 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    11641 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    11701 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    11761 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    11821 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    11881 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    11941 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    12001 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    12061 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    12121 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    12181 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    12241 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    12301 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    12361 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    12421 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    12481 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    12541 agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    12601 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    12661 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    12721 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    12781 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    12841 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    12901 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    12961 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    13021 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    13081 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    13141 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    13201 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    13261 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    13321 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    13381 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    13441 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    13501 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    13561 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    13621 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    13681 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    13741 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    13801 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    13861 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    13921 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    13981 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    14041 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    14101 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    14161 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    14221 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    14281 gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt
    14341 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    14401 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    14461 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    14521 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    14581 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    14641 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    14701 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    14761 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    14821 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    14881 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    14941 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    15001 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    15061 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    15121 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    15181 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    15241 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    15301 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    15361 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    15421 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    15481 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    15541 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    15601 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    15661 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    15721 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    15781 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    15841 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    15901 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    15961 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    16021 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    16081 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    16141 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    16201 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    16261 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    16321 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    16381 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    16441 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    16501 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    16561 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    16621 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    16681 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    16741 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    16801 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    16861 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    16921 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    16981 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    17041 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    17101 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    17161 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    17221 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    17281 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    17341 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    17401 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    17461 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    17521 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    17581 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    17641 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    17701 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    17761 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    17821 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    17881 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    17941 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    18001 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    18061 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    18121 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    18181 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    18241 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    18301 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    18361 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    18421 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    18481 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    18541 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    18601 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    18661 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    18721 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    18781 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    18841 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    18901 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    18961 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    19021 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    19081 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    19141 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    19201 cttgcacata tcgatcgaat ccgagataat acctacgctt atctgattgt agatgatttg
    19261 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    19321 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    19381 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    19441 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    19501 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    19561 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    19621 cttttattca aaaaacatac caaaaatatt ccaacatcat cttggacttt ctacatgtta
    19681 ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc tatcaatgaa gctctttttc
    19741 tttgcaatcc tcacttcaat cctctataat gtcgcatata gtttagtaaa tgagtaaact
    19801 actaatttac ttatttacta aaaataaaag gatgaggagt taaaataatg gaaataccaa
    19861 caaatttaaa acataaacca gtaatcgttg tagaagacta tgataaagtt gatggaagaa
    19921 atgcattaaa tacggatgca aaagggcttt cactgggcct tgctcagtgg aatgatcgtg
    19981 gaaaagtcga tatctcaggc aaggtatggc gacatactgg agaaaaatgg tcacgacagt
    20041 cggaagaatt accaatcaca cgtattttag atttagcgat cttagctgca caaggaagtt
    20101 tatacttcca agatgcttat cgttatgaaa aattttatga tccagaaaat ccagtcgtag
    20161 acatcattgg attacaaggg aaccgaatga cggtagaagt agataccaaa aatccaaaga
    20221 tcgatgaaga catcattttg tattacgata cactacaaaa agacggagaa ctgattgggg
    20281 aacgattccg tattttaaaa cgtcttttag atgagctagg atattaatag acagagatga
    20341 tcaaaagggg aagagaagca tggagaattt ctcactaaca gaaaaccgaa ccttttcacg
    20401 gctagaaaag caaatgatct ggaagaaacc gaaaagccat cggacaagtg cagaagaatg
    20461 gcggatcgcc tccgaaatca aagggaattg gcaagatcct gaaatgaaaa tcagtaacgt
    20521 acttttggaa ggagatgccg ggtcggggaa gacacaatta gccaaagcac tatctgctga
    20581 tctgcaactt ccctatacaa aagtgacctg ttttgcagat atggacaaat cagatgtttt
    20641 cggagcctta ttacctgtag tagattctaa taaggaacaa gaccaagaat tactagaagc
    20701 tatttaccaa acagatacac ttgaagctgt actgacactg atccagaacc attacggcat
    20761 tgatcctttt tctgctaaag aaaaattagc agagctagtt caaaggatcg aagctgatta
    20821 tccagatgca atccattaca gctattaccc ttctgaaatc gttcgagcga ttgaaaaagg
    20881 atatttatta gagattcaag agccgactgt catccgagat gcctctgttc ttgtggcact
    20941 taactcagcg ctcgaaccaa atgggatgtt gaatttaccg acaggaatca ttcgtcgcca
    21001 tccagattgc gtcattgtga tcactacaaa tcgaaattac caaggaaatc gcccattaaa
    21061 tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg gatcttcctg ccttagaagt
    21121 catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa caagaacttc tcttaaagat
    21181 ggcagagatt atccgtttgt tggatgaaac tgcaaaagct aacgccatca aaggtgtagc
    21241 aggaatgcgt tcttattttt attgggcaaa tacctataag caaggacaag atttactcca
    21301 gtcgatttat ccaaaagttt tgtataaatt atccactgat tcagatgaat tggcaattct
    21361 tgaacaagct ttgtctgaca gtggattgct actggaactt caggacatat tgagaaaaga
    21421 aaaatggggt catcctcaat ctgagcatac aaaaggaaga accattagcg ccgaagaagc
    21481 aaatgaacga aacatcactg aaaatgctga agaaattctg cgcttagatg aaaagaaatc
    21541 gttgtctgct gaagaaccaa atgagggaga agagaaacaa gaagaaacaa tagaaaaaaa
    21601 cgagattcct cctcaaatgc aaaaagaacg cagtagcgat atcgaaggaa aaaatcaaaa
    21661 agaacaacaa acaggagatg attctcaaga aatgtctgct tctgacatgg aagcacatga
    21721 taaggaaatc aagaagcaac tgaataaaga agcgcgacaa atcatgaagg aaacgattca
    21781 tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa ggaaaaacgc cggaagcacg
    21841 tgagctacac atgcaagtaa tgccaatagt agaatcactt agccggcaaa ttttagagtt
    21901 actggataat gaacaaacag agacgtacca aaaaggaaaa tacgaaggtc agcggttcaa
    21961 tgcaagcaaa gtagcatatg gtgatttgag aaattttgac aaaaagaacc cgccacatga
    22021 gcaaccttct ttagcggtag ctgtacggat cgatgaatct gggtcaatga tacgggatga
    22081 tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc gaattttcta agaaagtcga
    22141 tatcccattg ttgatttacg gagatacagc agaccgttcg tcaagagaaa agacttcttt
    22201 gttttcttat aaagaatttg aagacggatt tcaatggtta aatgaaaaat tagtcactat
    22261 gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt ttagcagcag aaaaattaaa
    22321 cagacaatcc gcaacaacca aactgttgct taatattagt gacggtcagc caaaagcgtt
    22381 gccggattat acaggcaaaa aagcaaaaga agatatccag tctgttttga aagaatatga
    22441 acgtcaagga atcctatttg tctccgcagc tattggccaa gacaaagaag aaatcaagtc
    22501 gatctacggt gaaagtcgtt ttgtagatat tactgacttg aatgaatttc ccaagcaaat
    22561 gattcaactg attgcccgct atctatgata aaataaagct caacgaacgc acttagcaga
    22621 ttcgttgagc ttttcgtttc tagttaccgg taacaaggat acttctaaaa tagaatcggt
    22681 tataatcaag agggaccgtt aaaggagtga aacgatatga tacaagaaca aaaatggtgg
    22741 aaagaagcag taggctatca aatctatcct gctagtttca aagatagcaa tggcgatggt
    22801 caaggagaca tcaatgggat ccgtgaaaaa cttaattatt tgaaagatct tggtatcggc
    22861 tttatctgga tcaatccgat ctatcaatct ccatttgtcg ataatggtta tgacgtaagt
    22921 gactatcaag atatagaaga gagattcgga acgatggaag attttgattt attgctaaaa
    22981 gaagcacatg aattagggat caaagtgatt atggatcttg tcatcaacca tagttcggat
    23041 cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa gcccgtatcg cgattattac
    23101 atttgggtcg acggggtaga tggcaaagaa ccgaataatt ggacatcgat tttcggcggt
    23161 tctgcttggg agtattctca tgaaactgga cagtattatc tgcatgtttt tgcaaaagaa
    23221 caaccagact taaattggga aagcgagaaa ttgaaagaag aactattcaa tatgatccga
    23281 tggtggttgg ataaagggat agatggattt cgtttagatg cgatttctca tgtaaaaaaa
    23341 gatgaatatt cagtaaaagc cacagagaac cctttctcac cctttcaaaa tgtctcagga
    23401 atcgaagaac atttgaccga tttgaaacat gtttttgaag agtacgatat catgactgtc
    23461 ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat gggtagggaa agacggttat
    23521 ttcgatatga tattcgaatt tgatcatatc catatctggc agcaagaaaa agaagggcaa
    23581 ttagatgtcc ttaaattgaa acttgcatta agcgcttggc aaacttcatt ggatggtatc
    23641 ggatggaacg ctttatatat ggaaaaccat gatgttcctc gtgccgtttc agtgtttgga
    23701 gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa tcgctatgat gtatttcttt
    23761 ttgcaaggta cgccatttat ttatcaagga caggaaatcg gaatgacgaa tatgcccttt
    23821 gaatcgatcg atcaagttga tgcagtagat tcgaaaagac tttataaaag gctgcttgcg
    23881 gaaggtaaga caagagaaga agcattggat atcattcgtg aaacaacgag agacaacagc
    23941 aggacaccca tgcaatggac ttcggaacaa tatgctggtt tttctacaca tgaaccttgg
    24001 ctgatcacta atccaaatac caaaacgatc aatgtagaac aacaagaata tgaaccagag
    24061 tcggtcctgc aattctataa gaacatgatc cgcattagac aaaccaataa aggtctgata
    24121 tacggttctt ataaagaata tcttcatgaa catccacaac tttatgtcta tgaaagatat
    24181 ttagaagatg aacaatatct tattatggta aacttaacag aatcacttgc tgactatgaa
    24241 cttccaaaag aagcagatca gtcctggaca ttactcttgt caaattcctc tagcggagaa
    24301 tttgaagcta aaggcatact agcaccttat gaagctcggt tatataaaac aaacaagtaa
    24361 aacttcctcg ttaaagttca ttagttggac tttgcgagga agtttttcag attgctctgt
    24421 caattactca tttcaataat tcgctctagg accaccgata tatttaggac gagagcgaag
    24481 ggctgtcacg tgagcttgtc caagggtttt caaacgaggt ctgacaggta cttggacatg
    24541 tttgatatgc ataccgattg atgtgtcacc gatatcgata ccggcttgtc caactacgtg
    24601 ttccacttcg acaggatcat tgaactggtc aaaagcagca acagaacaag cacctccagc
    24661 atgtagtgca ggaacaacag agacaatttc gaaatttttt gcttctgcca ctgttcgttc
    24721 tactactaat gctcggttca aatgttcaca accttggaca gctagcgcaa tttctttagg
    24781 atccaacaac tcttttaacg tacggatgat ccattgacca acttccgcac tggaattttt
    24841 tccaatatgt cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa gatcaccttt
    24901 tttcaagtgt gcctcagcca atatgtcatt aactatttca gtcaattggt cttttaattc
    24961 tttttctgac aacttcattt tctaaaacct tctttttcaa ttaatattgc ttccgtgctt
    25021 gaatacgttg aagacttgca gttataggaa aggcaagtcc cattccaatc agattttgga
    25081 agatgtttcc tggtatagaa gccactccag ccggccaatc atataaaatc caaccggcga
    25141 taccatagcc gatgatcata actaagcttc ctaataaaag ccctaatccc cacatacgag
    25201 tagaagattt gctgaaagaa ccagcaacat atccttgtaa cccgtggatc actaatgaaa
    25261 aaactgccca ctgcggatat cctgaaatca aatcgatcaa tagaccactg gaagcaccaa
    25321 caactaatcc acccgttgga ccataaagaa aagcagcggt atagatcccg gcctcgcata
    25381 atgtcacaaa tccattcgta gccggtacag ggatcaaaat tgttaatgaa agggcaactg
    25441 tcaacgcaat cagcatagca tagatcgtta attgcttagt tttcatttct cattcctcct
    25501 ttttgaatac cgccagacca gacacttccg gtatgatcgt tcatcagcac gccattagaa
    25561 atacaatggg caacaaattt tttactttct gctactgctt tggtaaaatc tgcccctaac
    25621 acaagattcg ctgttattgc agaagaaaag gcacaaccgg ccccgtttac tgttgtagtg
    25681 tcgatttttt tatgagaaaa tacctcataa tgatcaattg aatagagaag atccacagct
    25741 tcatctccag ccatccttgt gccgcctttg atcacaatgt tttttgttcc tgaagcagtg
    25801 agttctttag cgagatggat catttctgtt aacgaagagg gaagagcttg accagaaagt
    25861 aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag ttcttcgatg
    25921 agctgcgaaa tatacaattg attgatcaca gcttcagttt ctttgaatgc caaaacaggg
    25981 tctaaaatga ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac catgattgct
    26041 tcacttgaat tcaataaccc aattttgata ccatctaatg aagtcatttt atcaatcgaa
    26101 actaactgtc gtttaagcaa ttccaaagat atagattcca gcataaatcg ctcattaata
    26161 tctagtgccc ctatagaagt caaggcgctt agtccgaata gaccaaaatt ctcaaaagtt
    26221 tttaaatctg tttggattcc tccaccagca aatggatcag aaccgccaat tgtcaagatt
    26281 cttttcataa cattttcctc catgaataag agtataacga aattttcaaa aaagaaaaac
    26341 agccaattgg catattttta tctcatccaa tttaaaaaaa caaaaagaaa agaaagacca
    26401 aaatggatct ttctttcctt aaatagttag gacttttgtg attttaattc tttctcgatt
    26461 tgaagccgtt gcactttcat tgtagctgtt cgcgggattg catcaaaatc acggatgata
    26521 gggacgttca agcgaggaag ttcatggact tgctcccacc atcgattcca atccatttct
    26581 ttatcaggag ctaaagcaat aatcggttgt ggagaatgat ttttatccct tacaatcact
    26641 acttctgcca aaaaatctaa cgaatctaat aaaaagtctt caatagctaa tgtactattg
    26701 atcgtttcga tcaaatccac ttgccgatct ttcaaaaata ggtggccatg cttatccata
    26761 aaaccgtaat ctccgctatc ccaccaatcg ccataaacat tttcctggaa ccgtgcatct
    26821 tccttgtaat aagtcaaagc ccgtccttta gacagtaatt gtatatgacc atctgtgttt
    26881 tcaggtagga cattcccaac tgaatctgta atccgagcgc ttgtcatgtc ttccagtcca
    26941 acgcccatat ctcttgcatc acttgttttc aaactctcta gtgtatgagc cttaagaatc
    27001 attggtccgc attcactttg gccatatact tgaagaaata ccgcatcctg tgccatcgac
    27061 gttcgcaaaa atgtcttcat tgtctggttg tttatagcat caaaagtgga gtgatagtat
    27121 cggatacccg aaaaagcttc tggatgctcc ttagcagtaa aacgccactg aacaaagtta
    27181 ttcggatggg tctctagtgc aattggccgg tgagcttcca acatatgaac aacttttgaa
    27241 ctttgtgcgt ttgctaaagg catcatagga aatcccatgg acatcaaaga agagatacca
    27301 atattgaatc tggaatgcac tggagagatg tgaaaagcga ttaatttctt ttccgaaatt
    27361 tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca tagaatgagc agaatgacaa
    27421 ataagtttag ggataccagt tgtaccagaa gtatgggtca tataagaaat ttcatttttg
    27481 tctaaaaatg attgagaaac agacaaagca ggttgttcca ttaaataaag aataggaa
//
LOCUS       DADOOS010000022        26838 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.022, whole genome shotgun sequence.
ACCESSION   DADOOS010000022 DADOOS010000000
VERSION     DADOOS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.022"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            116..1384
                     /locus_tag="J8C37_000817"
     CDS             116..1384
                     /locus_tag="J8C37_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAZ5304276.1"
                     /translation="MGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVT
                     GTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAA
                     DPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAP
                     EAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVT
                     LHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQE
                     IVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRN
                     GTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFD
                     HRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1392..2798
                     /gene="lpdA"
                     /locus_tag="J8C37_000818"
     CDS             1392..2798
                     /gene="lpdA"
                     /locus_tag="J8C37_000818"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAZ5304277.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2960..3235
                     /locus_tag="J8C37_000819"
     CDS             2960..3235
                     /locus_tag="J8C37_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAZ5304278.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3241..4008
                     /locus_tag="J8C37_000820"
     CDS             3241..4008
                     /locus_tag="J8C37_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAZ5304279.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4176..6005
                     /gene="typA"
                     /locus_tag="J8C37_000821"
     CDS             4176..6005
                     /gene="typA"
                     /locus_tag="J8C37_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAZ5304280.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6266..8038
                     /gene="recQ"
                     /locus_tag="J8C37_000822"
     CDS             6266..8038
                     /gene="recQ"
                     /locus_tag="J8C37_000822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAZ5304281.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8152..8454
                     /locus_tag="J8C37_000823"
     CDS             8152..8454
                     /locus_tag="J8C37_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAZ5304282.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8457..9620
                     /locus_tag="J8C37_000824"
     CDS             8457..9620
                     /locus_tag="J8C37_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAZ5304283.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9646..13074
                     /locus_tag="J8C37_000825"
     CDS             9646..13074
                     /locus_tag="J8C37_000825"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAZ5304284.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13095..14219
                     /locus_tag="J8C37_000826"
     CDS             13095..14219
                     /locus_tag="J8C37_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304285.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14209..14646
                     /locus_tag="J8C37_000827"
     CDS             14209..14646
                     /locus_tag="J8C37_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAZ5304286.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14630..14968
                     /locus_tag="J8C37_000828"
     CDS             14630..14968
                     /locus_tag="J8C37_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAZ5304287.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15087..15647
                     /gene="rsmD"
                     /locus_tag="J8C37_000829"
     CDS             15087..15647
                     /gene="rsmD"
                     /locus_tag="J8C37_000829"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAZ5304288.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15644..16135
                     /gene="coaD"
                     /locus_tag="J8C37_000830"
     CDS             15644..16135
                     /gene="coaD"
                     /locus_tag="J8C37_000830"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAZ5304289.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16125..17168
                     /locus_tag="J8C37_000831"
     CDS             16125..17168
                     /locus_tag="J8C37_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAZ5304290.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17327..18055
                     /locus_tag="J8C37_000832"
     CDS             17327..18055
                     /locus_tag="J8C37_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAZ5304291.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18171..18416
                     /locus_tag="J8C37_000833"
     CDS             18171..18416
                     /locus_tag="J8C37_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ5304292.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18413..18631
                     /locus_tag="J8C37_000834"
     CDS             18413..18631
                     /locus_tag="J8C37_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAZ5304293.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18767..19306
                     /locus_tag="J8C37_000835"
     CDS             18767..19306
                     /locus_tag="J8C37_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAZ5304294.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19299..21572
                     /locus_tag="J8C37_000836"
     CDS             19299..21572
                     /locus_tag="J8C37_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAZ5304295.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21610..22656
                     /gene="holA"
                     /locus_tag="J8C37_000837"
     CDS             21610..22656
                     /gene="holA"
                     /locus_tag="J8C37_000837"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAZ5304296.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22745..22999)
                     /locus_tag="J8C37_000838"
     CDS             complement(22745..22999)
                     /locus_tag="J8C37_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAZ5304297.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23399..23668)
                     /locus_tag="J8C37_000839"
     CDS             complement(23399..23668)
                     /locus_tag="J8C37_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304298.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24138..24593)
                     /locus_tag="J8C37_000840"
     CDS             complement(24138..24593)
                     /locus_tag="J8C37_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAZ5304299.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24731..26659)
                     /locus_tag="J8C37_000841"
     CDS             complement(24731..26659)
                     /locus_tag="J8C37_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAZ5304300.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8943 a         4656 c         5669 g         7570 t
ORIGIN      
        1 cagtggtgta gccgcagaat cccaaactcc agctaaaccg gctgctgaac ccactgttga
       61 tacagaatca gctggatcgt caagcgaagg agtcttccaa tttaagttac cggatatcgg
      121 tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa
      181 tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa ttccgtcacc
      241 tgtcacaggg acagttaaaa acgtgattgt tccagaaggc actgtagcaa atgttgggga
      301 tgtattagta gaaatcgatg ctcctggaca caatagtgca ccgtcaacta gtgcgccatc
      361 agctgaagca ccaaaagaaa aagtggaaac atcaggttca gcaagtgtcg ttgaagctgc
      421 cgatccaaat aaacgtgtat tggctatgcc atctgttcgt caatttgccc gtgaaaaaga
      481 tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc agagtaacaa aagaggacat
      541 cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg aaatcagaag cgccagaagc
      601 ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa ccagctgcac cagcaaaacc
      661 attcaagtcc aacttaggag acttggaaga acgtgtagca atgacgccaa cacgtaaagc
      721 tatcgctaaa gcaatggtca acagtaagca tacagctcca cacgtgacat tgcatgatga
      781 agtagaagtg tctaaactat gggataatcg gaaacgattt aaagaagttg ctgctgcaaa
      841 tggtacgaaa cttactttct tgccatatgt cgtgaaagct ttaacagcga ctgttaagaa
      901 atacccagta ttgaatgcat ctatcgacga tgcaaaccaa gaaatcgttt ataaacatta
      961 ctacaatatc ggtattgcaa ctgatacgga tcacggacta tatgtaccaa atgtgaaaga
     1021 tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc aatgagaaag caaaattagc
     1081 tcatgacggc aaactatctg cagaagatat gcgcaatgga acaattacga tcagcaatat
     1141 cggatctgta ggtggcggtt ggtttacacc agtcatcaac tatccagaag tagctatttt
     1201 aggtgtagga acgattgcac aacaaccaat cgtaaatgct gaaggcgaaa tcgttgtcgg
     1261 tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc gtagatggag caactgcgca
     1321 acaagcaatg aataatatca aacgtctttt agctgatcca gagctattaa tgatggaagg
     1381 atgataaaca aatggtagtt ggagattttg ctgtagaatt agatacagtt gtaattggtg
     1441 cgggacctgg tggctatgta gctgccatcc gcgctgctga aatgggacaa aaagtagcaa
     1501 tcattgaaag agagtatatt ggcggtgttt gtttgaatgt cggctgtatt ccttcaaaag
     1561 cactgatagc tgcaggacac cattatcaag agtcactaga ttctgaaatg tttggagtaa
     1621 ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga atggaaagaa aacaaagttg
     1681 tccatacgtt gacttctggt gttggtttcc tattaaaaaa acataaggtt gaaacaatcg
     1741 aaggagaagc gttcttcgta gatgatcaca cactacgcgt cattcatcca gattcagctc
     1801 aaacttattc gttcaataac gcaatcatcg caacaggttc ccgtccaatc gagattccag
     1861 gatttaaatt cggaggacgt gtgttagatt ctactggcgg attagcattg aaagaagtac
     1921 ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc cgaattgggc ggagcgtacg
     1981 caaacttagg agcagaagta acgattcttg aaggctcacc acaaatttta ccaacttacg
     2041 aaaaagatct agtaaaatta gtagaagatg atttcaagaa aaaaggcgta acagtcgtaa
     2101 cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag cgtgacagtg aaatatgcag
     2161 tggacgggaa agaagaatcc gttactgctg attatgtgat ggtcactgta ggtcgtcgtc
     2221 cgaatacaga tgatatgggc ttagaacaag cgggtgtcga agtaggagaa cgaggattga
     2281 tcacggtgga taaacaagga cgtacgaacg tacctaacat ctatgccatc ggtgatatcg
     2341 ttccaggggc ggcattagca cacaaagcca gttatgaagc aaaaatcgct gctgaagcaa
     2401 tctctggcaa aaaagtagct gtagattata aagcaatgcc ggcagtagcc ttcactgatc
     2461 ctgaattggc ttctgtaggt atgacgatca aagaagcaaa agatgctggt atcgaagcaa
     2521 aagcatataa attcccattt tcagggaacg gtcgggcatt atcgcttggt aaaacagaag
     2581 gattcatccg tctagttacg acgatcgaag ataacgtgct tattggcgcg caaatcggtg
     2641 gagtcggcgc aagtgatatg gtctctgaat tagctttagc aatcgaatca ggaatgaatg
     2701 ctgaagatat cgccttgacg attcatcctc atccatctct tggcgaaatc gtcatggatg
     2761 cttcggaatt agcattaggt ttaccaatcc atatttgatt tgaataatac aggcgaaacg
     2821 caactgaaaa atcagttgcg ttttgttttt tagaattatc aataatatta ttatgtaaac
     2881 ttaaaaatat gacaacttct tggtttatta ccacttccta tgctacactg aagaggaata
     2941 tgaaatagga gagttgaaga tgcaagaata tcagtatcca cttgatttag attggacaac
     3001 agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg gaacgtgcga atgaaaaagg
     3061 gataaacaat gaagcttttt tgaagactta tcagcagttt aaaactgttg tcaaatccat
     3121 tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct tccggttatt ctttatatcg
     3181 tacagtgcag caagcaaaaa aaaatccaaa caaaactttg agaatgaacg ggtgagataa
     3241 atggaacaga tgattgatca tatcaagtcc tggcttgaag aagctggtga gaaaatcaaa
     3301 tcttctcaac aaacattaac aatctcgcaa aaatcaaatc gaacggatct agtaacaaat
     3361 gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc attcctttga tccagaggcg
     3421 aaaatattgg cagaagaaga agggtgtaat caattatcgg atctcgatgg aagagtcttt
     3481 atcatcgatc ctattgatgg caccttgaat tttgtgattg aaggggaaaa tttttgtatt
     3541 atgctcgctg tttatgaatc cggcgttgga aagcttggtt tcatttatga cgtgatgaga
     3601 aatgaacttt tttggggtgg aaaaggaata ggcgtgtata aaaatgatga gccattgcct
     3661 agaccagcaa ataaatcctt atcggaaggc ttacttggtg tgaatagtta tcttttcggt
     3721 cataataaat atggaatccg agaaattgcg gaaagcagca tggggacacg tatgtacgga
     3781 tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc acattgctta tatttcaaac
     3841 cttagccctt gggattatgc agcgggaaat gtcttattgg aagagtttgg tatggtaaca
     3901 agcggtcttt ttggtactcc tttgacattt gaaaaaaggg aatactattt agcagggact
     3961 tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag aaggctgaaa aaaaggtaga
     4021 aatcattcac aaaaatgata aaaacgagca aaagattaat tttctcgaaa aaatgaaaac
     4081 attttactat atttcatgaa aaaagtctgc tataattagc agtcgagtaa aatttgccct
     4141 gtcataaaga accaataaaa aaggagttag ttacattgaa ttatagaaac gatatccgta
     4201 acgtcgcaat tattgcccac gttgaccacg gaaaaacaac attagttgat gaactattaa
     4261 aacaatcaga tacattagac gcacatacgc aattgcaaga acgtgcgatg gactcaaatg
     4321 cgctagaaaa agaacgtggt atcacgattt tagccaaaaa cacagctgta gactataaag
     4381 gtattcgcgt aaacatcatg gatactccag gacacgcgga cttcggtggc gaagtagaac
     4441 ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga tgcgtatgaa ggaacaatgc
     4501 ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca tatcacacca atcgttgtcg
     4561 taaacaagat tgacaaacct tctgctcgtc ctgagcatgt cgtagatgaa gttcttgaac
     4621 tattcatcga attaggagca gatgatgatc agttagactt tcctgtgatt tatgcatctg
     4681 cactaaatgg tacgtcaagc ttgtcagatg atccagcaga tcaagaacca accatggcac
     4741 ctatttttga tacaattatt gaaaaaatcc ctgctcctgt cgataacagc gacgaaccat
     4801 tacaattcca agtttcatta cttgactaca acgattatgt aggtcgtatc ggaatcggac
     4861 gtgtatttag aggaacaatc aaagtaggag accaagtagc attaattaaa ttagacggaa
     4921 cagttaagaa attccgtgta acgaaattat ttggtttctt tggactaaaa cgtctagaga
     4981 ttcaagaagc aaaagctgga gacttgattg ctgtctcagg tatggaagat atctttgtcg
     5041 gtgaaacagt tactcctgtg gatcatcaag atgcgttacc tatccttcac attgacgaac
     5101 caacattgca aatgactttc ttagttaata attcaccatt tgccggtcga gaaggtaaat
     5161 tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga acttcaaaca gatgtatcct
     5221 tacgagttga accaacaaac tctccagatg catggacagt ttctggtcgt ggagaattgc
     5281 atttgtctat cttgattgaa aacatgcgcc gtgagggata cgaattgcaa gtatctcgtc
     5341 cagaagttat tgaaaaagaa atcgacggtg taaaatgcga accgtttgaa cgcgtgcaaa
     5401 tcgatacacc agaagaatat atgggtagtg ttatcgagtc cttgagctta cgtaaaggtg
     5461 aaatgcaaga tatggtccat acaggaaacg gacaaatccg tttgacattc ttaacacctg
     5521 cccgcggttt gattggttat tcaactgaat tcctttcaat gacacgtggt tacggaatca
     5581 tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg tcaaattggc ggacgtcacc
     5641 aaggggcgct tgtttcgatc gatacaggaa aagcaacgac ttattctatc atgagtatcg
     5701 aagaacgcgg tacagtcttc gtagaaccag gtacagaagt atatgaagga atgattatcg
     5761 gggaaaacag ccgtgataat gatttgactg tcaacatcac gaaagcaaaa caaatgacaa
     5821 acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa aaaaccaaaa caattgacgt
     5881 tggaagaatc tttggaattc ttgaatgatg atgaatattg cgaagtaaca ccagaaagca
     5941 ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga aaaagcatct aaaaagaaaa
     6001 aataatctgt aaaaggatga agagagaaac cagcttgtta taaaaaatag ctggtttctt
     6061 tttttgtcca actcttttct gtggagaagt catctttgtc aatggacata aattcagaaa
     6121 atacgccgaa agacgtttaa aagactttta aaaaacttat ttctagagag tttttgtttc
     6181 ataataagat aatgtttcac gaaaggagat caacttgtcc ttcgaagaaa atcgaagtaa
     6241 aatggcaaga gaaaggagcg ggaagatgaa cgacttatta aaacaatact ttggttatga
     6301 tgagtttaga cctggtcaaa aagaaattat ccagaaagtc attgatcagg aaaatgtatt
     6361 aggaatcatg cctaccggaa gtgggaaatc aatctgttac cagcttccag ctttactatt
     6421 agatgggctt actgtggttg tgtccccttt aatctctttg atgaaggatc aggtggacgc
     6481 ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc tcgttagatg gttacgaaac
     6541 agctagacgc ttccaagaaa tcgatagaca gcagtaccgt ttactttata tcgctcctga
     6601 acgttttatc atgccggatt ttatccaagc aatgaagcga tggaacgtcc gtatgatcgc
     6661 tattgacgag gcacattgta tctctcagtg ggggcatgat ttccgcccaa gttatttaca
     6721 gatggcgaac cagctagatc aactgccaaa tcgtcctgta attgttgctt tgacagctac
     6781 agcgactgtg caagtagctg ctgacataaa aagactttta aaaattccag aaaacaacca
     6841 tattcagact ggttttgaac gagaaaatct acgatttcaa gtaattaaag atcaaaaaaa
     6901 agaacaatat ctaatcgaat atttaaagat caacaaaaat caatctggta tcatttatgc
     6961 agcaacgaga aaagaagtag accgtctcta tcatttgctt aaaaaattcg atttttccgt
     7021 gggtagatat catggtggtc taaatgagaa tgagcgaaca gaaatgcaag aagccttttt
     7081 atacgatcgc ttgcagctga ttgtagcgac aaatgcattt ggtatgggta taaataaaag
     7141 caatgtccgt tttgttatcc attatcaaat tcctggctct ctagaagcat actatcagga
     7201 agctggtcgg gcgggaagag atggtctttc cagtgaagcc atcctgcttt tctctcctca
     7261 agatatccaa gtccaaaagt tttttatcca gcagtcccaa agagaagaag ggcaaaagca
     7321 aaaagaatat gaaaaattaa aagcgatgac agaatacgtg tatattgagt cctgtcttca
     7381 gcaatacatc ctaaactatt ttggcgagac aagcagtccg tgcaaccgtt gtggtaactg
     7441 tttagatgac agagaaatag tagaagtaac gacagaagca cagatggttc tttcttgctt
     7501 aaaacgtatg ggtgagaact atggtaaaca aatgctgatg aaagtcttag caggatcgaa
     7561 agaacaaaag ctgcaagctt taggttttgg acatttatct acctatggac tgatgaaaaa
     7621 ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg atatcgaatg gctatcttct
     7681 gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa agagggatac aagtattgaa
     7741 aggacaagaa tctgtttatc gaaaggaacc caagaaagtc cagcagctat cggatgaaga
     7801 aaccgataca ctatttgaag tgctgcggga actgcgaacg gatctagcat cagaagcagg
     7861 cgttcccccg tatgtcgtat tttcagattc cacattgaaa gaaatgagcc gaatacgtcc
     7921 atcaagcaga ttggagatgc ttcagataaa aggagttggc caaagcaagc ttgataaata
     7981 tggcgaagcc tttttatctc ggataaaaaa tgcggactct aacgttctgg ataagtagaa
     8041 agaacgaata cgtaaaaatg aataaaaaca actggttaaa tctgtttttt ttgctcctta
     8101 tgaaaatcaa atatgttata attaaggaat tagaatggga ggtaagcaca gatggattct
     8161 ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag cagaacggat caaacgatta
     8221 atccgaaatc aaaacaacag tctctgtatc tcacaatgca aagccttcga agaagtggta
     8281 gatacacaga tgtacggctt ttcccggcaa gtcggttttg ctgtccgtat aggagtaatc
     8341 ggaaagaacg agggccagct gatgttaagt gagctagaac gagaattgaa tcagttatat
     8401 agtgaggttt accaagaaat ttatgataaa aaagaagttg gcaaggagga atagacttgc
     8461 ctaagaaagt gaaaaaaagg catctattag actatagcat cttgattccc tatttaattt
     8521 tatgtgtatt gggattgatc atggtatata gttctacgtc gtatctatta ttagaaaacg
     8581 gacaaaatcc gtccgcatca gtcattaacc agagtatttt ttgggtgctt agcttgattg
     8641 ctatcgcact actttataaa atgaaaacag atgttttgaa aaaccagcgt ttgatcatgg
     8701 cagccatcgc agttttgacg attttgctgc tgattgttgt gttttttggt aaggagatca
     8761 atggggcgaa aggttggctg caaattgcag gattctcgat ccaacctgcc gaatacctaa
     8821 aaatcatttc tatctggtac ctgtctctga ctttatctaa aagacaaaac agtgtacaaa
     8881 aagattttct tgggactgtg aaacgtccac ttgcaatggt tatcggactt actgcattag
     8941 tcgctattct tcccgatttt ggtaatgcgg cagttatctt tttgattatt ttggttctgc
     9001 ttttagccag cggagtcaat tatgtgtata ctttgattgt aggagtagga ggcttttgtc
     9061 taagtacttt tacgatctgg ctgatcaata tcacaaacgg aaagatctta cccggacgtt
     9121 tacagtatat ctataatcga tttgctattt accaaaatcc tttctcagac gaattaaata
     9181 aagggcatca actagtcaac ggctactatg ccatgttcaa cggaggacta tttggcaggg
     9241 gattaggtaa cagtatccag aaaaaaggat ttttacaaga agcacaaacc gattttattt
     9301 atgcaatcgt agtggaagaa ctgggagtga ttatggggat tttgatatta gcgttgctat
     9361 tctttatgat tgtgcgcatt gtccttgtag gtatccgttc aaaagatccg ttcaattcgc
     9421 ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt atttgtcaat cttggaggaa
     9481 ttactggcgt tattcctttg acagggatca cgttcccatt tttaagtcaa ggaggctcca
     9541 gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa catcagtgcg gatgaaaaac
     9601 gcaagagctt aggtttataa taaaaaaaga tgtaggagtg gttcgatgaa aaaagtttta
     9661 gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg cttgtacaga attaggaatt
     9721 aaaactgttg gaatctatgc agaagaagat gaatattccg tacaccgttt caaagcggat
     9781 gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg cgtatctaga tattgaaggg
     9841 atcatttcta tcgcgaaaga atgcggagct gacgctattc atccgggtta cggtcttttg
     9901 tcggaaaact tgaattttgc gcaacggtgc gaagaagaag gcatcatttt tgttggaccc
     9961 aaactgcatc atttagatat ttttggagat aaaatcaaag caaaagctgc agctatcgaa
    10021 gcggggattg cttcgattcc aggcacagat ggaccaatcg cttctataga cgatgccttg
    10081 gagtttgcga aacaatacgg ttatccgatt atgatcaagg cagctctagg cggcggcgga
    10141 cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag agggctacga acgtgcgaaa
    10201 agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg ttgaaaaata catcgctaat
    10261 cctaagcata tcgaagtaca gattctcggg gatacgcatg gaaacgtcat ccatcttttt
    10321 gaaagagatt gttctgttca gcgccgtcac caaaaagtag tagaagtagc gccttgtgtc
    10381 tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag ctgtccaatt gatgaaatat
    10441 gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg aaggagatga tttttacttt
    10501 atcgaagtca accctcgcgt ccaagtggaa catacgatca cagaaatgat tacagatatt
    10561 gatatcgtaa caacgcaact gttgattgct caaggtttgg atctgcataa agaaatcgga
    10621 ttgcctcagc aagaaggaat taaattaaat ggttcagcca ttcaatgccg aatcactaca
    10681 gaagacccgt tgaataactt tttaccagac acagggaaaa tcgatacgta tcgttcacct
    10741 ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg cgggctatgt agtgacaccg
    10801 tattttgatt cattgctagt aaaagtatgt acgcatggtg ctacctttga aacagccatt
    10861 cagaaaatgg aacgttgctt gaaagaattt agaatccgtg gagtgaaaac aaacattcca
    10921 tttatgctca atgtcatcac acatcctgaa ttccaatcag gaaatgcaaa aacaaccttc
    10981 attgatagta cagcaacatt atttgaattt cctcgactac gagaccgcgg aaacaaaacg
    11041 atgaaatata tcggtgaaat tactgtgaat ggttttccag gtatcgaaag tggcgaaaaa
    11101 ccattttatg aagaaccacg tatgcctaaa gatctaatca caagaactga ttacgtaacg
    11161 gctaagaatg tattagatgc gaagggtgcc gatgctttgg tcgaatggat caaaggacag
    11221 gaaaatttgt tgttgacgga tacgacattt agagacgccc atcaaagtct gctagcaact
    11281 cgtgttcgga caaaggattt caagcagatt gcacgtttga ctggagaagg tcttcctgaa
    11341 ctcttttcaa gtgagatgtg ggggggagca acatttgatg tggcttaccg ctttttgaat
    11401 gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa tgcctaatac gttgctgcaa
    11461 atgcttttca gaggttcaaa tgccgttggg tattctaatt atcctgacaa tgtattggtg
    11521 gaatttgtca aagaagcagc agcgcaagga atcgatgtat ttcgaatctt tgatagtttg
    11581 aactggactc cacaaatgga aaaaagtatc caagctgtac gggatactgg taaaatcgca
    11641 gaggcagcta tttgctatac tggcgatatc aacgatccaa gtcgggcaaa atacaatgtt
    11701 cagtattata aagacatggc aaaggaacta gaacaactgg gtgcgcatat cattgcaatc
    11761 aaagatatgg ctgggctgct taaaccgcag gcagcttatc gtttgattag tgaattgaaa
    11821 gaaacgacag atctgccaat ccatctgcat actcatgata ccagcgggaa tggaatcatc
    11881 acttattctg ctgcttcaaa agcaggagta gacatcgttg acgtggcaat gagtgcaatg
    11941 agtgggaata ctagtcagcc tagcatgagc agcttgtatt atgcactggt caatggtcca
    12001 cggctgccag aaatcacgat tgaaaatgca cagaaattaa accattattg ggaagatgtg
    12061 cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc ctgaaacaga agtatatatg
    12121 catgaaatgc caggaggaca gtattcgaat ctacagcaac aggcaaaagc tgtcggatta
    12181 ggtcatcgct gggatgaaat caaacaaatg tatcatactg tcaacttgat gtttggcgat
    12241 atcgttaaag taactccatc atctaaagta gttggagata tggcactatt tatggtccaa
    12301 aatgatctta cagaagaaga tatttatgaa aaaggcgaaa cactaagttt ccctgaatct
    12361 gttgtgactt tcttccaagg agaactaggg caacccgttg gcggatttcc taaaaaattg
    12421 caaaagatta ttcttaaagg cagacctgca ttgaatgaac gtccaggtct tttggctgaa
    12481 tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa aaattggcta tgaaccaaaa
    12541 caagacgaag tattaagcta tttgatgtat ccgcaagtct ttttggacta tcaaaaagca
    12601 tataatcaat ttggcgatgt tacattactg gacacaccaa catttttcca aggtatacgt
    12661 ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa cgcttatcat tcgtttagat
    12721 gaaatcggag aacccgatat cgaagggaat cgcgtactgt tcttcaattt gaatggacaa
    12781 agacgtgaaa tcactatcaa agataattcg atcatcagta cggtccaaac ccgattgaaa
    12841 gctgaaccga caaaccgaga acaaattgga gcaacgatgt ctggttctgt tttggatgta
    12901 ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt tgatgattac tgaagcgatg
    12961 aaaatggaaa cggccatcga agcacgtttt gacggagaag tcgcacatgt atatgtgtct
    13021 tcaggagaca ctatttcgtc tggtgacctt ttgattgaag tgactgagaa ataataagcg
    13081 aaaggtgggg aaacatgaaa agactgggtt tgttttggct gattctgcta ttagttattg
    13141 tggcattcta catggaacca gtcttgtttc cgccaaaact tgaagtgaag gaaaactctg
    13201 atcaacaaat atcaaaccaa atgacaacgt ggaaatacca ggagcttaaa gcaactggtt
    13261 tttctactta tatcggaaaa ccgatcgatc agctggaaaa ggattttggc caagcctatg
    13321 atcgattaac tagcggattt ggttttgaaa caagatatta tgaaaataaa gctagtcatc
    13381 tcacatttga agcgaacatc caagacagca gagtcacaac tgttaaagta ctgcaaacag
    13441 ggcctgctga tatcgcgccg ttttatttag ggatgacgat gcaagatctt acgaaaatca
    13501 ctactatcta tactaatttc acttttgagt accatgatac agaagttgga atcgaactaa
    13561 tggaagagga tatgaattat cgtccgctaa ttgcttttga taacgacact tttgctattt
    13621 tattttttga tcaaacaaca aacggcctat tttcagttgt gtatttagat aaagatagtc
    13681 ttttgaaact attgccatat caagtgtttg gtgaaggcct tccacattat caagcagata
    13741 aatcggctga ttgggaatca atcaatcgcg ttaaagaacg aaaaagtacg actttattaa
    13801 atatgcttag aagagaagac gaacttccca tatacaatca aacacttgcc tttcaaaacc
    13861 acagtcagct gctactacat gattttttga caaggcctga ggaaatattg tcagaggaac
    13921 gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac gacagtgaaa ttctcactga
    13981 cgaatgatga attggagaag ttaatcagta aacaaaaatt agaaaatact agtgggctat
    14041 ttattcatcc ggcgattgac gctacttttt cgtttttata ttggtacagc gatccttaca
    14101 accatgaccg tttcatggac ccgggaacgg attctgtagg cattgctttt tctaaagaaa
    14161 atatgttagt cttattgcaa gaagaaaacg gagaaagtag tgatagcagt gatcgttaac
    14221 gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg ctgaaatgat aaaaaagagt
    14281 aaaacgatgg cttcttatct tgaacataaa cagtcaatgg aagaatctgc aaaagtttgc
    14341 cagttgaaag aagcgttctt gtctaagaaa gaagcatttg aaaatgtagc ggcatatggt
    14401 gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc gaaaagcaaa aagacagctt
    14461 gatatggatg agcatgttgc agctttccga tatagtgaaa cacagctgca aagtctgttg
    14521 gatcaaattg tccaagtact tgccgaagaa gtttctaatg aaatcaaagt agatgcaggt
    14581 agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag gtaattgtca tgcaattaga
    14641 caatagtgaa tttgaaataa caaaacgacg cgggctgatc gtatgggtct acagcttaaa
    14701 acaattaaag aatctgaaaa agttcggctt gttgcattat gtctcaagaa gaatgaaata
    14761 tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca gaagaacgaa tccgtaaatt
    14821 acattttgtt cgaaacgtag aacgttctta ccgaccggat atcgaaatga attttgccga
    14881 aaaaatagga aacaaaacag tattaaaaga cgaaggtttc gaaattgaag agctaaatac
    14941 agaaatccga ttagctgaac atctttaaaa atctcttatg agcagttttt aaataaaaaa
    15001 taacttggat aacgatcgat ttttgatcat tgtccaagta tttttttggt atactagtac
    15061 cagtaccatt tctaaggagt gagtagatgc ggataatatc aggaaattat ggcgggcgcc
    15121 gtttaaaaag tttagcaggg gcgaacacca gaccaactac agataaagta aaagaatcca
    15181 tattcaatat gattggacca tattttgatg gagagacagt attggatctg tttgcaggaa
    15241 gcggtggttt agctatcgaa gcagtatcta gaggctgcag tcatgcggta tgcgtagaca
    15301 aaaattacca agcgctaaaa atcatcaaag aaaacatcga aattacgaaa gaaccagaga
    15361 aatttaccac aataaaactt gatgcggaca aagcaatcga acggctagca gcggagaaac
    15421 aacaattcga ctacttgttt cttgatccgc catatgcaaa acaaaaaata atcgatcaaa
    15481 tccataaaat ggaagaactg ggactttttt ctaaaaccgc agttatcgta tgcgaaacgg
    15541 ataaaacagt cgaattgcca gaaactgtgg ctggattcca tcaaataaga aaacagattt
    15601 atggcataac ggccgtaact atctatagaa aagagggaaa tcaatgaaac gtgcattatt
    15661 cccaggaagt tttgatcctt ttacaaaagg tcacttagat acggtggaac gtgcggctaa
    15721 attattcgat gaggtagtaa ttggtgtttt tatcaatact tcaaagaaaa gtttgtttcc
    15781 cccagaagaa agaatgacat tgatcacaaa agctgtttct catcttccta atgtaaaagt
    15841 catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa gagattggtg cagatgcgtt
    15901 gatcagaggc atacgaagta tcaaagactt tgaatatgaa cgagagattg ctcagatgaa
    15961 tcatcatcta tatgatgaat tggaaactgt cttcttatta gcaaaaccag aatatagtca
    16021 cgtgagttcc agtattttaa aagaagtact gcactttggc ggagatgtga gcagctactt
    16081 accgccagtg atcaacgaag ctttagctgg aaagcgtgaa tcaaatgaaa gctaaacaat
    16141 ggttgaaaac agtactgatt atcatatttg gaatgttgat cattggattt gtagtagttc
    16201 ccttgccgta ttatgtagaa tcaccgggag ccacgatcga tctgaaagaa ctaattacgg
    16261 tcaatgataa ggaagataaa ggttctgggt ctttctctct tacctctgtt ggtatcaggc
    16321 aagctacagg tttcttagct ttaaaagcta aaatgacccc ttttgaagaa ttgatcagtg
    16381 aagaagaatt gactggtggg gcgacaaatg aagaatataa tcaaatgcaa cagtattata
    16441 tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa acttgcagac aagccttata
    16501 aaatggaatt caaaggcgta tatgtcatga gtgtagagaa aacatcgaat ttctttggca
    16561 agctttcagt tggagataca gtaacaaaag tagacgggaa aagctttcaa agtacagaag
    16621 catttatgga ttatgtaaaa agccaaaaag ttggacaaac aatcgaaatc agttatttgc
    16681 ataatggtga agaaaaaaca gcttctggtg atctgatcga gttaccaaca gataaaaagg
    16741 caggaatagg tatcacgctt actgaccata ccgagatcga atcagataca gatgttcgta
    16801 ttaattctgg ctctatcggt gggccatcag cgggattgat gttcacttta gaaatctatg
    16861 aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat tgcaggaact ggaacgatca
    16921 acagtgaagg agaagttggc cgaatcggag gaatcgataa aaaagtggca agtgcggata
    16981 aagctggcgt agagattttc tttgcaccag atgatacgat ttcagacgat gttaagaaag
    17041 aatatcctga aataaaaacc aactacgaag aagcaaaagc tgccgctaaa aaattgaatt
    17101 cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc gcttgactat ctagagaaaa
    17161 tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga gttctgtcac aacccctctt
    17221 tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga aaaaattgac gtaattagat
    17281 gttgaaggga cgattttgcc agagctcact gtttcttcaa ttttaaatga aaggagtaat
    17341 gaatgatttt tactggtcat tctttgaaaa aagaacaatg atagaaaaaa tcagcaagaa
    17401 aatactagct tcacccaaat tgatcatttg gatgtctatg atagttctta tcttttgtat
    17461 cgcaagtggt tttttattat caaatttgct attttataga gaaacagaag agactgctgc
    17521 aaaagaaata ctttcttcgt ttagttccag tggagaagat ccatcagctg aaggaaacga
    17581 tgtagaagag gatgctcgtc caaaggtcat gtacaccgat atcaaaggaa gtgtgaagga
    17641 gcctggtatc tattcctttt cttcagaaga acgggtctat gatgtactaa agcgtgcagg
    17701 tggattatta gaagaagcgg atagcgatcg aatcaacttt tctgcgaaga tagaagatca
    17761 gcaagtccta tatatccctg ctgtaggtga agagctgcca gaacatcttg atcagttggc
    17821 atcctcggaa ggaaaacagc ctacagcaga ttccgagcct cccaaaataa atatcaatac
    17881 agccactcca tcagaacttc agcagattcc tgggatcgga tcagtcaaag cgcaagaaat
    17941 cattcgattt agagaagaaa acggatcctt ccagaaagtg gaagacctgc aggagatttc
    18001 tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc gttacaatta aatagaatga
    18061 ttcaaaatcg actggcacga gcaataaatg gacgctaatg gatactaaga ataatccaat
    18121 tctaagagct tggcaggacg aggaggtagt atttttggat aggaaatagc ttgattgttc
    18181 gcaaagaaac attaaaaaaa ccaatgctga atgtctatct ccaaaataaa atatctggga
    18241 ttcatattat gaatacggct gtcagcggca ataacagtca agctctacga gaacgctttg
    18301 ccaaagatgt acttagctat acagcagata aagtatttat tctcattggg acgaatgatc
    18361 tagcagagca taaacaactt tctaaagaaa cgtaccaaaa aatctgcagt ggatgatagt
    18421 tcagctgaaa aaagactata gttctgatca tgctttcttt atcacgccac cagcagtcga
    18481 tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa gaatatatct ctgttctcac
    18541 agaagtagca aaacgccatg attgtgaagt atcgattttt atacggaatt tcttgaacaa
    18601 atggattcga agaattttta taatgggcta atggatgacg gacttcattt cggcagtgaa
    18661 gggtaggatc ttttgtcaga caggatgatt cgttatattt aaagaatatt ttgtttaagc
    18721 aatgagtact ttttaatcaa cttgaaaaaa cggaggaata taaaacatgt tagaaaaatc
    18781 ttcaaaagca tgcgatacga ataaagaaaa agttactgat aagtcacata atgaacgcat
    18841 tccttgggac cagtacttta tggctcaggc agtactgtta tctttacgta gtacgtgtac
    18901 acgtttagaa gtaggagcga cactcgttcg agaaaaaagg atcattgctg gcggctataa
    18961 tggagcagtc agtggagatg tccactgcat agatgaaggg tgttacgtcg tggatggaca
    19021 ctgcttgcga acgatccatg cagagatgaa tgcactccta cagtgtgcaa aacttggtat
    19081 cccgacggaa ggttcagaaa tctatgtcac acattttcca tgtttggcct gtacaaaggc
    19141 tttgttgcaa gctggtgtga aagggatcaa ttatctccat gactatcgaa atgatcctta
    19201 tgcccaagcg ttgatcgaac aattaggtgt atctgtacac caagttgctt tagactcaca
    19261 gcatttttca aaacttcaaa atgaattaac aggaaaacat gtctagcgtt ttagaaaggc
    19321 atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat gattatctgt ttgttgtatt
    19381 acactgacaa ttggggttat cgtttgcttc ttgccggaat ttttttatgg ttttcgctaa
    19441 aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag cataacggta tccatttctt
    19501 ataggctaca agagtattca tttttagagg aaaaaacaac atctgcgcaa attactgtat
    19561 ttacagatac gattcgtgca aatggtaatt ttttgacaat ggaaggaagg ttatcagaga
    19621 ataaacaaaa gctccagctt tcttatcaag taaacacaga agcagaattg aaaacatggc
    19681 ttaacttccg aggaaaggga atagaattga ctgctgaagg cgagttcatc tctccagcaa
    19741 aaaagcggaa tctttataat tttgaccaag aagcttactt caggaaccat agaatttctg
    19801 gacggttcca aattcaaaag attgaacaga tcaccatgca aaatcactgg cgaaactggc
    19861 ttcagagaaa aagaggatct tttatctcct ttgtccaatc tgaatttccg caaaaaacaa
    19921 gtgtatatat taattcttta ttatggggct ataaagatgc aggattcaga gaaccagaag
    19981 aaatttttag ggagagtggt ttgctgcatc tgttcagtct atcgggtcta cacatccaat
    20041 tttatttagg gtggctgtat tatttatttc gacgtatcgg atggccacta cacgtcagta
    20101 ttattccgtt gagtctacta actatctgtt atgtttctat tgcaggaagt tctatttctg
    20161 ttttgcgtgc tagtttgctg tttttattgc gattgctagt caaattgctg aataagcatt
    20221 attccaccat ggacttattt gcagttgtct tttggcttct tttactattt gatcctcgat
    20281 ttctttttca tgcgggtgga cagcttagtt ttttcatgag ttttttgttt atcctgattt
    20341 cttttgatgg agattggttg aaaaaaacgg cctctcacgt cctgtttact atgatcacga
    20401 tgccgttgat tgtatggcat ttttatgagt ggcctatttt aggtagtctt tttacattac
    20461 tgattgcacc ggtattcaag tttctttttc ttcctggtgt gttatttctc acactcttca
    20521 atcgctggct gccggaaact ttacttttgc tgatagaaga gggattaatc ttatttgagc
    20581 gaataatcga tttttcttca gggtcgtctc tcattatagg acgtttgccc cttgtgctat
    20641 ctgggatgta tattttcgga ttactgattt gtatggatag gttgaaagaa caacctgtga
    20701 aacacttgtt ttatgttggt cttttttctt ttattttttt gactttgcca tttttacgct
    20761 tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga cagtatcttt ttgcaaagtc
    20821 cttttcgaaa agaaacgatt ttgatcgata ctggaggtca gttacacttt gaaagagaaa
    20881 agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa tatcgttcca tatctaaaag
    20941 gtcaggggat aagtgtaatc gataaactca tcttgacaca cgacgatacg gatcatgtgg
    21001 gagaattacc cacgttatct cagcatttta ctatcaaaaa aatttatatc ggttggggcg
    21061 ctggacgaaa tgagccatta caaaggcatt tatctgaagc tcaaaaaaat ggtactgaga
    21121 tcattgaaat caaacatggg gacaggatca gcggttatta tgatctttat gtattgactc
    21181 cttttgaaaa gggagaggga agaaatgaag attcgattgg tctatggatg gaatataaca
    21241 ataggcgctt tttattttta ggagatttga atcaagcaat ggaaaaacaa ttgcttttga
    21301 tttatcctaa tctgaaagcc gatgtagtca aattaggtca tcacggtagc cgtacgtcaa
    21361 gtaacacaga ttttctttcc catattgaag caaaacatgg gattatatcc tgcggcgtaa
    21421 ataatcgtta tggtcacccg cacagcgaag tattggagac actagaagaa aatcagataa
    21481 aaacatggcg aacggatcaa caaggaatga tcacctatcg ctggcatcca gtttttcatc
    21541 ctcatgggct agtggaaaca atgatagact agtaattctc atctgctcat ggtaggataa
    21601 tttttgaaag tggtgagtaa tttgagtata caagcagagt tacaaaaaat acgtaaagaa
    21661 aaacttgcat cttgttactt gattttagga acagaaaagt ttctccaaga tcaagttcgg
    21721 acagaaattg tcaaaaaaat ccaattagag ggcgaagatg acttaaattt tttaagtttt
    21781 gacatggaaa atgcaagttt agatgaggta gtagctgaag cagagacgtt gccgtttttc
    21841 ggagatcaac ggttagtatt cgtagagaat ccttattttt taacaggtga aaaagtaaac
    21901 aatggaatcg aacaaaatac tgatttattg acggcttatt tgaaagaacc attagaatcg
    21961 actgtattag tattctttgc gccatatgaa aagttggatg aacggaaaaa agtaacgaaa
    22021 caattgaaaa aaacagcaat tatcatcaat gtccaacagt tgaatgaaaa agaagtacgt
    22081 caatatctga tgaatacctt agagaattcc tcaatcaaaa tggaacgaag tgcaattgat
    22141 ttattcttga ggttgaccga cttagattta tccaaaatga tgagagagtt acagaaattg
    22201 gtcctttatg gacaaaatca acagcaaatc acggtaagag aagtagaaga attagttcct
    22261 aaaacgttag aacataatat ttttgacatg acccaatata ttttaaaagg gaagacggaa
    22321 caagctttgc gtttatacga agatttagtt atgcaagggg aagaaacaat caaaattaat
    22381 gcgattttgc tttctcaatt acgtttattt ttacagacaa aatttcttgt aaagattggt
    22441 tatcagcagg caaatattgc tgaaacattg aaaatccatc catatcgtgt aaaacttgcc
    22501 atgcaggaag taaaaaaatt cgatgaacat ttgctggtac agttgtttga tcaattagtt
    22561 gagatggatt atcaaatcaa aacgggacaa atcgaaaaag aattaagttt tcaactattc
    22621 gttttgcgca cagggcagtt gttaagaaat cgataagaag aaagacgagg gcaaagcatg
    22681 cgggaaggtc gtttgttttt gtcctttttt tgacaaataa aaaaatcggc cgaagccgat
    22741 tcttttattt agcgattttt ttgcttaaac gagatttgtc gcggttagct ttgtttttgt
    22801 ggattagacc ttttgattca gccatatcaa tagctttaac agcttcttta tataacgcat
    22861 ctacgttgtc agcacctgaa gctacagcat cttcaaattt tttgattgca gtacgcatag
    22921 cgcttgtttg agatgaattt ttcacattag cgttttcgct tgtacgaaca cgtttgattg
    22981 cagattcaat gtttggcatt agtttcacct ccgataactt aagttcttac gcgtgttacc
    23041 gcataattca acattcatta ttatacctaa aggcagcgtg ctttgcaata tgaacatgac
    23101 aagttttttt ccttaacagt cttttattag gatttttttt cagtaaaaac ggatagtaca
    23161 agctgaaaaa aatcaaaaag acgctattgt gggaggcaat agcgtcaaac caaactagat
    23221 ttttatttat ctatttaaac aaagtttgaa tgggaatgtt tggtactaag ttattatata
    23281 acaaagagaa ccgtttgcaa agtttttttt gaaaagggat acgattccct gttgttcttt
    23341 gtttttctat taagaaactg cagttatttt ataaccagta tcctttgatc agtaaaggct
    23401 attctggtgt accgattatt ttaatgtatt catcgatatt ttttatcaac atttttttta
    23461 tgaccaactg gttccgacgg ttccatttgc cacttacttc cagcacatat ttatttaggg
    23521 gcaaacgtaa gatatatttt gccaaatagc gatctttaag caggccattg atcacacggt
    23581 cctcaagctg caagttgatc ccagtcagca tcattggctg ttcttgtaaa acgtacgtat
    23641 caattaaaag accttcaaaa gtttccacaa gcgtcttcct ttcttttcaa agtaacctga
    23701 aaacaggcac taattcttcc attttgatat tcgttgaagt ggatgcgatc attctttctt
    23761 ttgtccactg taatctagta taacaaaaaa gagcagaaga gccaaaaaga aaaaagagca
    23821 tcgaattcta taggaagtct cattggcacg gcctaaatta ttacaaaaca ttgattaaaa
    23881 aaagcccctg catcgcagga gcatccccta tatcgtttta ataggtcaat caaaaagcag
    23941 tatcaaacac attttgtatc aaatcaggaa ataatagata agctaagccc agaatcaaaa
    24001 gtaccgctag aagaaaccag ccgattgttc ctatgaaata ctttaatttt tcgaacattg
    24061 tatcacctcg ttgtcagtat atcacggatc agtaaattgg gggtgttaag aatctcctaa
    24121 aatagacaaa atttgttcta ttttcctaaa gcaaatactt gtaagataca gtaagtgata
    24181 ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga agacaacacc taaaatcgtg
    24241 ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa tcaatcctac acccttggaa
    24301 gataagacag ccactagccc accgcaaaaa acagctaccc agccaatagg ggatttaaag
    24361 gcttctaata gatcacgtag accgatttct cctgttgcga tagggattag aatagccgct
    24421 gtgatgatcg tcaccccaat attgattcct ttgccttgaa gccaagggaa aaaggaacgg
    24481 gaggctggta tgagcttcat aatcaaaaca aaagctgccg ctatcatcag actttgattt
    24541 ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa gccaactttc catgaaatat
    24601 attcctcact ttcttcgaaa caatcataac atgtttttag aaagttacaa aagtaggaat
    24661 tcatccttca agacctattc ataagctgtt tttttcataa aataaacaaa atcataaaaa
    24721 aacactttgc ttatttgaca gaagacagcc gcgctttgta tagtggacaa gacttacatt
    24781 tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa acgcaagcca ttcagaataa
    24841 ccaatattgt gcttccttcg tgtcctacaa ctccaagagg tagatttaaa aattgcagga
    24901 agttagaagc aatcaacaag agaataacaa gaatcgaaaa aataatattt tgtttgatga
    24961 tccgccgcat ttttaaagat agccgatgac tgtagacaaa tttatttaag tcgcttttca
    25021 ttaagacgat gtctgcgata tccatagcgg catctgtccc ttgccccata gcgacaccta
    25081 aagaagcggc tgctaaagca ggcgcatcat tgactccatc accaaccatc gcatttactt
    25141 ggtaaagttc attctctttt ttgactagtt gtgttttttc ttccggagta cagcttgcat
    25201 agtaatgttc aatccctact tgttcagcaa tggcctttgc tgtgccagaa tggtcgccgg
    25261 ttatcatact tgtatggatg ttttgggatt taaagtagga aattacttgt tgggtatttg
    25321 cttttggaat atctagcaga cctaaaacag caattaactg ctgatcttta gaaagataaa
    25381 tcacggtttt gccttgattt tccaaacgtt cgatcatttc cgcaatctct ttgctgatta
    25441 tcatcgtctc aggatcatat gcgtgcttgc caactttcca ttgcgcacct aaataggtag
    25501 tttgtaatcc aaaaccagta atttcttcta ttggtaagta ttggatttct tcaggtattg
    25561 tgcaatcgaa atattgcaaa atcgcatgag caagtggatg cgtcgttttt tgttccatag
    25621 caacaagaag ttgttgtgcc aaatcctttt catcaaagaa aaagctgtca gttacaactg
    25681 gttttccttt agtgagcgtt cctgttttat caaaggcaat cgcttttaat tcagaaagct
    25741 tttctaaatg gataccgcct ttgattaaaa tcccattttt tgcagaattt gaaagagcag
    25801 ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg agaagccaca actaataata
    25861 ccattcctcg ataaaagctt tcatcccaac tccaacctag gaaaaagtgg gggagaagga
    25921 tcattagagg aacagcaagc aaaatcaatt ttacataatt attttcgatt cgttcgataa
    25981 atgaagctgt ttgagttggc gtattctgcg cttcttctac taattggata atcttagcaa
    26041 agacagtttc ggaactcgtt tttgttactc gaatcgtgat gggatttcct tgattcagcg
    26101 ttcctgcata catctcatcg cctaaatatt tttctacagg cacagattca ccgcttaatg
    26161 cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc atctatcgga acagctgctc
    26221 ctttagcgac aaataccaag ttatccacct ttagttgaga aacatctact tcttgcattt
    26281 gtccattttc ttttaataat tgggcttttt gtggttgaag attcatcaaa gcagtaattt
    26341 ctttttgact tttattagtt gtgtattcct ctaatgcacc gcttaaacaa aagatgaatg
    26401 tcaacattgc tccttcaaag taattaccga tcaaacaggc accaatagca gctaaagcca
    26461 tcaataaatc cacattcagt gtccgatcat tccacaattc tttcaagcct tccgatgttt
    26521 gtttatatcc accgatgata atggcactga tataaaaaag tggggtgatg gtaagagaga
    26581 atcggtcgaa gataaaaccg aagatcataa ataaaaggca gccgattgtt gctgcaaaag
    26641 cttttttctg attagtcatc gttattagac ctccttgtca aaacaaggat agcataataa
    26701 aaatgtaatt gataataata acagctaaat gagaacgata atggttatca tttggtgatt
    26761 aagcttgatg ttgtttttat tatttataat gattattatt aaagaaaata aaaaaccaga
    26821 acatttctca aataagag
//
LOCUS       DADOOS010000023        26426 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.023, whole genome shotgun sequence.
ACCESSION   DADOOS010000023 DADOOS010000000
VERSION     DADOOS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.023"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(130..825)
                     /locus_tag="J8C37_000842"
     CDS             complement(130..825)
                     /locus_tag="J8C37_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAZ5304301.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="J8C37_000843"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="J8C37_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAZ5304302.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="J8C37_000844"
     CDS             complement(2024..2215)
                     /locus_tag="J8C37_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAZ5304303.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="J8C37_000845"
     CDS             complement(2236..3126)
                     /locus_tag="J8C37_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAZ5304304.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="J8C37_000846"
     CDS             complement(3113..3880)
                     /locus_tag="J8C37_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ5304305.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="J8C37_000847"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="J8C37_000847"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAZ5304306.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="J8C37_000848"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="J8C37_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAZ5304307.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="J8C37_000849"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="J8C37_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAZ5304308.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="J8C37_000850"
     CDS             8496..8834
                     /locus_tag="J8C37_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304309.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="J8C37_000851"
     CDS             complement(9002..9760)
                     /locus_tag="J8C37_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAZ5304310.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="J8C37_000852"
     CDS             complement(9778..10596)
                     /locus_tag="J8C37_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAZ5304311.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="J8C37_000853"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="J8C37_000853"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAZ5304312.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="J8C37_000854"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="J8C37_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAZ5304313.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="J8C37_000855"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="J8C37_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAZ5304314.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="J8C37_000856"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="J8C37_000856"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAZ5304315.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="J8C37_000857"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="J8C37_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAZ5304316.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="J8C37_000858"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="J8C37_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAZ5304317.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="J8C37_000859"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="J8C37_000859"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAZ5304318.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="J8C37_000860"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="J8C37_000860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAZ5304319.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="J8C37_000861"
     CDS             20638..20937
                     /locus_tag="J8C37_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAZ5304320.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="J8C37_000862"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="J8C37_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ5304321.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="J8C37_000863"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="J8C37_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAZ5304322.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="J8C37_000864"
     CDS             21904..23877
                     /locus_tag="J8C37_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAZ5304323.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="J8C37_000865"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="J8C37_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAZ5304324.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="J8C37_000866"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="J8C37_000866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAZ5304325.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         8016 a         5214 c         5235 g         7961 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    26221 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    26281 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    26341 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    26401 acgcccaatc aaacgaaaaa tatgag
//
LOCUS       DADOOS010000024        26418 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.024, whole genome shotgun sequence.
ACCESSION   DADOOS010000024 DADOOS010000000
VERSION     DADOOS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.024"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(290..658)
                     /locus_tag="J8C37_000867"
     CDS             complement(290..658)
                     /locus_tag="J8C37_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAZ5304326.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            808..1290
                     /locus_tag="J8C37_000868"
     CDS             808..1290
                     /locus_tag="J8C37_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAZ5304327.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1327..2337
                     /locus_tag="J8C37_000869"
     CDS             1327..2337
                     /locus_tag="J8C37_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAZ5304328.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2452..3165)
                     /locus_tag="J8C37_000870"
     CDS             complement(2452..3165)
                     /locus_tag="J8C37_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304329.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3355..3945)
                     /locus_tag="J8C37_000871"
     CDS             complement(3355..3945)
                     /locus_tag="J8C37_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAZ5304330.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4429..4656
                     /locus_tag="J8C37_000872"
     CDS             4429..4656
                     /locus_tag="J8C37_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAZ5304331.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4672..5838
                     /locus_tag="J8C37_000873"
     CDS             4672..5838
                     /locus_tag="J8C37_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAZ5304332.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6072..7115
                     /gene="hrcA"
                     /locus_tag="J8C37_000874"
     CDS             6072..7115
                     /gene="hrcA"
                     /locus_tag="J8C37_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAZ5304333.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7209..7772
                     /gene="grpE"
                     /locus_tag="J8C37_000875"
     CDS             7209..7772
                     /gene="grpE"
                     /locus_tag="J8C37_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAZ5304334.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7823..9652
                     /gene="dnaK"
                     /locus_tag="J8C37_000876"
     CDS             7823..9652
                     /gene="dnaK"
                     /locus_tag="J8C37_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAZ5304335.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9803..10969
                     /gene="dnaJ"
                     /locus_tag="J8C37_000877"
     CDS             9803..10969
                     /gene="dnaJ"
                     /locus_tag="J8C37_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAZ5304336.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11051..11716)
                     /locus_tag="J8C37_000878"
     CDS             complement(11051..11716)
                     /locus_tag="J8C37_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAZ5304337.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11692..11865
                     /locus_tag="J8C37_000879"
     CDS             11692..11865
                     /locus_tag="J8C37_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ5304338.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11910..12647)
                     /locus_tag="J8C37_000880"
     CDS             complement(11910..12647)
                     /locus_tag="J8C37_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304339.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12649..14151)
                     /locus_tag="J8C37_000881"
     CDS             complement(12649..14151)
                     /locus_tag="J8C37_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ5304340.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14410..16404
                     /gene="uvrB"
                     /locus_tag="J8C37_000882"
     CDS             14410..16404
                     /gene="uvrB"
                     /locus_tag="J8C37_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAZ5304341.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16414..19230
                     /gene="uvrA"
                     /locus_tag="J8C37_000883"
     CDS             16414..19230
                     /gene="uvrA"
                     /locus_tag="J8C37_000883"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAZ5304342.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19493..20758
                     /locus_tag="J8C37_000884"
     CDS             19493..20758
                     /locus_tag="J8C37_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAZ5304343.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20762..21493
                     /locus_tag="J8C37_000885"
     CDS             20762..21493
                     /locus_tag="J8C37_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAZ5304344.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            21628..22512
                     /gene="rapZ"
                     /locus_tag="J8C37_000886"
     CDS             21628..22512
                     /gene="rapZ"
                     /locus_tag="J8C37_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAZ5304345.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22509..23507
                     /locus_tag="J8C37_000887"
     CDS             22509..23507
                     /locus_tag="J8C37_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAZ5304346.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23541..24476
                     /gene="whiA"
                     /locus_tag="J8C37_000888"
     CDS             23541..24476
                     /gene="whiA"
                     /locus_tag="J8C37_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAZ5304347.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25019..25669
                     /locus_tag="J8C37_000889"
     CDS             25019..25669
                     /locus_tag="J8C37_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304348.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            25856..>26418
                     /locus_tag="J8C37_000890"
     CDS             25856..>26418
                     /locus_tag="J8C37_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAZ5304349.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8726 a         4455 c         5610 g         7627 t
ORIGIN      
        1 ctgaaaataa tgggcagtat tatgaaagat cactgatatt ttctcgtatt ttttccttat
       61 tcaaaagttt tgcaggaaaa ttgctgaaaa aataatttta ctatgtagaa aattcaaagc
      121 cattcaatca gtgatttttt tcagcacaag aaagactcct tataaaaata gcagatactt
      181 tgtcaattaa gtgtttaacc agggatgata aaaaggatag gattgaacaa aacctgtata
      241 aggtgatgaa tagtttcctc gccagtaaaa ggtttattcg tataaagctt tatcgccaaa
      301 atttatcatg acctttttta tcttcaatat taaatgaaat caattgttca atcaattccc
      361 agttgattac acttttccaa ggaactttga tgacgttatc cgtatatacg tagcctgctt
      421 cttggatatt tttttcaagg gcacgaatcg ctgctttttc cggtgcgata gaaaaatgat
      481 ttttagcaaa actaaatgca ataataaagg taccatggtc agtaaacata ggttggttcc
      541 attttacagt tgtgtctagc tggggatacg tttctgttat cttagcaaaa aggtctttga
      601 tcttttgttg atgttctttt tctttgattt gattgatgat cggctgaaat ttttccattc
      661 taaaacgcct ccattaagtg gtttgattta ttttaccaga aaaacaatag ttatgtataa
      721 aaaacgaaca aaacacaagt aatgttgtaa ccgctatatt tatatgataa gatgtctcaa
      781 ataataaaat agaaaagaga cataaaaatg attgacttgg caaagaaatt cttacacgaa
      841 aatatatatt ttgagttgta tcatcatcga gccatttata caaatgaaga tgctttggta
      901 ataaagaaag aacaaggatt tacaggtaca gagaccaaaa gcttattctt aaaagataaa
      961 aaagataata actacgtata tctgactttt actacgaaac aaacggattt caaagcatta
     1021 gggaagatcg tcggtaaaag gctttctatt gtttcatcag aacgcatgga agaacaaacg
     1081 ggacaaaagc agggcgctgt ttctccgttt ggttatgaaa aacagattcc gattattatt
     1141 gatgaagagc tcttatcaaa tgaaaaactt gtttttgcac caggtagacc agatcaaacg
     1201 atggtcatcc aaacggttga cttaaacaaa atcatccagc tacttgagat gccgatattt
     1261 ttgatgccgg caaacgaaaa aaacaattaa ccaagtaaaa tagtgcatac ttggaggtga
     1321 agcaacatga taagttatcc attattagaa agaaatgcaa cgattggggt tactgcacca
     1381 tcctcaggcg tatcagagga acaccattcg ttactcgaat cagctttaaa gagaatggaa
     1441 gaaaaatctt tccaagtagt tactggaaag acgctttgga cgcaagataa ggaaaaatca
     1501 ggtccagcaa gaaaacgagc agatgaattc aatcaaatga tgcaagatga aaatatccaa
     1561 atgatctttc cgacttgggg aggagaatta ttgattgaga ttctcgattt tgtcgatttt
     1621 gatgctctac aaccaaaatg gatagtaggt tattcagata ttagtacgtt gctacttgct
     1681 gtcacattaa aaacagggat agccactgca catggaacta accttattga tttaagagga
     1741 gagtacttgg atgaaacgac agcaatgtgg gaaagtgtac tgaaaacaag agaaggagaa
     1801 tcgattctac agcaatcttc tgaaaagtat caaaaagtgt ggccccatga gcattcatca
     1861 tcttctgttt tccatctcac tgaaccaaca aattggcaat cagtcagcgg agatgcagta
     1921 aggatgaagg gacgattatt aggtggttgt attgatacga tcaggcattt aatcggtact
     1981 ccttatggag atgtccaatc ttttaaaaga aagtataatg atggaaagcc gatcttgtgg
     2041 tttctagaaa actgtgagct atcgtcagtt gatttaagaa gatcacttgt tcagatgaaa
     2101 ctggcaggct ggtttgatgg cagctcgggg atcatgttcg gtagaagtgc ggcagaggat
     2161 ttgttagatt ataaaatgaa ggatgtatac gaagatctgt caaaggaatt aaaactaccg
     2221 atcttgtatg atattgattg tggtcatgta ccgccacaac tgacattgat taatggtgcc
     2281 tatgcagaag tagcggtaga agatagaaaa gcgactgttt tgcaaacgtt tatttaaaaa
     2341 attttttcct gccttggagt ctatcaaaga ccaataaatc taaatagaaa agaaagtgaa
     2401 agaagctgac aaaatgctgt cagcttcttt ttgtataaca tattagaaag tctaattatc
     2461 tataactgtc acatcgatta actgtttcac gtcattggga tttcctgtag gaatagctac
     2521 tacagatgca ttatcaatga tttcttgagt tgtttctcta atgaacgctg gataagatgt
     2581 ttgatctgca ggcatttgcc aatgtgaagg aactggataa ctagtgattg tcccgtcatg
     2641 gttacttgaa tacactaaaa ctccctggta actgtatttc ccagtgagca taaccacatc
     2701 tttcggatag actgcactcc ctgcatcata aggggcgata ggtgttcctg caggataatg
     2761 aataacatgt aattcgaaat cacccgatgt atctctaaaa gtattacctc taatagctag
     2821 ccaaacacgt gcgtactcaa tttgtagatc agaatatcct gctaacggat ctattgactg
     2881 agaagactgt gaggaggttg tgtctattgt tgaagaagaa atatttgtct caactgtact
     2941 actagatgta gaagaagtat tgggctctga gactttactg cttgttgaag atgtattcgt
     3001 ttcagtagaa gctgatttct gagtacttga tttttcagct gaactggttt gtctgattga
     3061 acttttgtct gtggtcgcag atggatcact attttcttta ctaggagacg tacatcctac
     3121 aaggaataaa gagaccaaca ataaaataaa acctaacttt ttcatataaa atacctcatt
     3181 atttttttaa taccagtata gcataatcct gaatttttga atccgtaaaa ccttgtctat
     3241 gaatataaat agagtgtatc ttctttgata aatgtcagaa aaagtgtgac aaataatcat
     3301 cagtaaaatt acttgtcaca ctattgataa ggagtattta attgaagaga atgattagga
     3361 gaaaggatct ccgtactttt tatagtaatc atagaatcca agtttgtata gacgaatcgt
     3421 tttttctctt atttcttctg tttcaaaatg gactgcttga agaatcaagt ctgtgaattc
     3481 tataggagct gttccattcg cagtaatcaa attacgatct ttcacagctt gttgttctat
     3541 aaaatcttct ttattctgat aattttccga ttctaaccaa ggaaattgcg catttccggt
     3601 gtgtttgtaa ccttgcaata atccgctctg agctaaataa tcaactgccc cacaaatagc
     3661 agctactggt acgtctgttt ttaacaattt atcgattatt tgatataaat gatgattttt
     3721 tatgtcccat gaattgccac cgatcataat aaaaaggtca actttttcag gtatttgttc
     3781 gattaagtaa tcgatttttg tttttaatcc tccaatggaa gtgacctctg attggataga
     3841 agcagttttg ttttgccatt tctcattttg gtttagtgtg ctagtaatat aagcaccttc
     3901 ccaatcggca tattcctcta atagaaacgt aatcgcagtt ttcattttca agctctcctt
     3961 tactatctat aacaaggaca acagttttaa cctacagaca ttcattaaaa ttgggtagcg
     4021 acagcgaaaa caacaattct taagttttag acagagtata cagatagtca acttaattaa
     4081 atacgaacta aataaaaatg acaggtaatc atttttgttt accagcatta taacattttg
     4141 catacatgga tgacaaaaga accaattccc acttgcttga gtacaatatt aaaagaaact
     4201 ggaaggcaaa aagtaattat agaaacgaaa aataaatcat atcgggcact cagacatgaa
     4261 aacaacccta gagatatata actaggtaac cagtttaatg aaagaaaaaa tcatccaaga
     4321 agtcgattct tggatttttt agtggatttg ttcgccaaat acgtttagta tgcgtatatg
     4381 agaggttgta agcaaagcgt tgtggtctga gcacttaagg aggaatctgt gaaaatagct
     4441 tttcatattt ttgcagattt tgacttaatg ccgaaggtca gctttgcgat taccgtttat
     4501 cgagaggctg taaaagcacc gtgtagctcc gatgacaata agcaagcatt tttagagcta
     4561 ggtttccttg attctaaata tgcggcttat ttcaatgagt tggtgcttga acgcgatttt
     4621 aagaaggtcg aaaacaggca cgacttttcg acgtaaggag aaagaatttc aatgatagaa
     4681 acaataaatc ccgcagaaaa caaggtttct gcgtacattc atatcccatt ttgcgagcat
     4741 atctgctatt actgtgattt taataaagtt tttttagaag ggcagccagt agatgagtat
     4801 gtggaaatgc tgctgaatga aatgaaaata atgctagaaa gtcatccgac agcgaattta
     4861 gaaacacttt acgtaggggg cgggacacca acttctttat ctgctaaaca acttgatcga
     4921 ctgttatcag gtgctagaga gatcctgcct tttcaaaatg gtaatgagtt tactgtagaa
     4981 gcgaatccag gtgatttgac ccgtgaaaaa cttcaagtaa tgaaaaacta tggcgtcaat
     5041 cgtttatcca tgggagtgca gacatttgat aatcgcttat tgaaaaaaat tggacgtaaa
     5101 cacaccgcag aagatgtgta tcaaactatg caatttttag aagcagaaaa ttttaccaat
     5161 gtaagcatcg atttgattta tgcattaccg ggacaaacac tagaaggata tctggatact
     5221 ttggaaaaag ctttggcttt ggatctgcct cactattctt tgtactcatt gatcctcgaa
     5281 aacaaaacaa tgttcatgaa ctgggtacga caaggaagac tcgaactgcc tgatcaggaa
     5341 acggaaacca gaatgtttga agagacgatt gaagcaatgg aaaagcatgg acgtcaccaa
     5401 tatgaaataa gtaactttgg gctctccgga catgaatctc aacataacct catgtactgg
     5461 aacaatgacc actattttgg gtttggagct ggtgctagcg gttatcttgg agatattcgt
     5521 taccgcaaca aaggaccaat ccaacactat ttacgcccat tacgagcaaa tgaacgcccg
     5581 gtattggaag aagaaaaatt gacacgaaaa aatcaaatag aagaagaaat gttcttagga
     5641 ctaagaaaaa aaacagggat tactaaaaaa catttctatg atcgctatca aaaaacaatt
     5701 gaatcggtgt acggtgatgt gctaagggaa ttagagcaag aagggttact tgttaatgaa
     5761 gaagaccgtg tttatttgac accaagaggg acctttttgg ggaacaatgt gtttgagcgc
     5821 tttttgtttg aaaaatagtt taaagaatat ttgtttttta tctgaattta aataaaaaaa
     5881 gttcaaaaat acaataaagg actattgaaa cgttgatata actggattta catcaattat
     5941 ttagaattag cactcttgtg tttagagtgc taaaaaatgg agtctttctc ttgacaggag
     6001 aagggcttct tgctatattt ataagtggat tagcacttag aatatatgag tgctaatgag
     6061 aggtgaggaa gatgttaaca aagcgacaaa acgacatttt acgtctgatc atccaacact
     6121 atacgagtag tggcgtgccc gttggttcca aaacattgat gtctgaggga gtcaaggcca
     6181 gctcagcaac gattcgcaat gatatgaaag cattagaaga tgaaggattg ctgttgaaga
     6241 ctcactcttc ttctgggcgg attccatctg ctttaggcta tcgttattac gtggatcatt
     6301 tgttaaaacc tgctcgcgta gcagaagatg atttgctgct gattcgtcag tctctaggca
     6361 gagaatttca tgaaatcaat gagattatta agcaatctgc tgagatatta tctgagctga
     6421 caagctatac gactttttca ttgggacctg aaataaaaga ccgccgactg actggctttc
     6481 gtatcgtacc cttgaataat cgacagatta ttgctatcgt cgtaacagat atggggaatg
     6541 tagaaagcca agtgttcact cttcccgaat atatgggaag tcaggaacta gaaaaaatgg
     6601 tacagattgt aaacgaccgt ctagtcggag atcctctgat gacggtgtac cataaacttc
     6661 gtacggagat tccgatgatt cttcataaat atttccaaac gacagaaggg atgtctcagt
     6721 tatttgacac cgttcttggt catgtttttg aagataaggt ttatgtcagt ggacaaatga
     6781 atctcttgga ttatgagccc cgtcaagatc ttgatcagtt caaatcgatg ttttctttta
     6841 tgaaagactc agacggactt acacaaatga tcactccgat ggatagcaat attcatatca
     6901 agattggtgc tgaattaggc aatgatttat tgcagaatat gagtatgatt caagcaagtt
     6961 acgagataga tggtcatgga agaggtacaa tagccttgtt agggcctact agtatgcctt
     7021 attctcggat gttcggtcta gtcgatgttt atcgaagaga actagcagct aaacttgctg
     7081 attactaccg ttcgcttgac ttgtcaaact cgtaatgtga ataacgaagc tatttgcagc
     7141 tgatcgttct ctctttctta gtaagagcaa tcaaacagct gactgaataa tgaaaggaag
     7201 actgtatcgt gaaagaaaat caagaagaac tagataaaga aatgacagat gctcagccag
     7261 aaccggaaat cgatgtcgaa gctgcagaag attcgggtat ttcagaagct gaagcggaag
     7321 aattcgaaac agcgaaatta aaagctgagt tagaagaaat ggaagataaa tacttgcggg
     7381 ctcgtgctga aatcgcaaat atggcaaatc gaggaaaaaa tgagcgtgag caattacaaa
     7441 aataccgttc acaagattta gccaaaaaat tacttccatc aattgacaac ctggaacgtg
     7501 cattagccac agaagtgtca gatgatcaag gggcaggttt gaaaaaaggc gtggaaatgg
     7561 tcttggaaag tttgcgtaac gcactggaag aagaaggaat tgaaaaaatc cctgctaaag
     7621 gagaagcctt tgatcctaat cttcatcaag cagtacaaac tgttccagcg actgaagata
     7681 caccagccga tacgattgta gaagtactac aagaaggcta caaattacat gatcgcgtgt
     7741 taagaccaac catggtcatt gttgcacaat aattgtgcgg atataaaact tacactttca
     7801 attaaagaag gagacgttaa ttatgagtaa aattatcggt attgacttag gtacgactaa
     7861 ctctgcagta gcagtattgg aaggaaacga agcaaaaatc atcgctaacc cagaaggaaa
     7921 ccgtacaaca ccttctgttg tatcattcaa aaatggagaa atccaagtag gggaagttgc
     7981 aaaacgtcaa gctgtgacaa acccaaatac gatttcatca atcaaacgcc atatcggtga
     8041 agcaggttac aaagtagatg tagaaggtaa atcatataca ccacaagaaa tctcagcaat
     8101 gatccttcaa tatatcaaag gattcgcaga agactattta ggtgaaaaag tcgaaaaagc
     8161 agttatcact gtgccagctt actttaatga tgcacaacgt caagcaacaa aagatgctgg
     8221 taagatcgct ggtctagaag tagaacgtat cgtcaatgaa ccaactgcag ctgctcttgc
     8281 ctatggttta gataaaacag atcgcgacga aaaaatctta gtattcgacc ttggtggcgg
     8341 tacttttgac gtatctatcc ttgaactagg agacggtgtc tttgatgtat tgtcaacagc
     8401 tggcgacaac catctaggtg gggacgactt tgataacaaa atcatcgact acatggtagc
     8461 agaattcaaa aaagaaaaca gcattgactt gtctcaagac aaaatggctt tacaacgctt
     8521 gaaagacgct gctgaaaaag cgaaaaaaga tttatctggc gtatcaagca cacaaatcag
     8581 cttgccattt atcacagcag gagaatctgg accattgcat ttggaaatga cattgacacg
     8641 tgcaaaattt gatgaattaa cagctgactt agtagaacgt acaaaagtac cagtacgtca
     8701 agcgctaaaa gatgcaggtt tatcacaatc agaaatcgac gaagttattt tagtcggtgg
     8761 atctactcgt atccctgcag ttgtagaagc agtacgtaaa gaaacaggaa aagaaccaaa
     8821 caaatcagtt aaccctgatg aagtagttgc tatgggtgct gctatccaag gtggcgtgat
     8881 cactggtgat gtgaaagatg ttgttttact tgacgttaca ccattgtcat taggtatcga
     8941 aacaatgggt ggagtgttca ctaaattgat tgaccgcaat acaacgattc caacaagtaa
     9001 atcacaagta ttctcaactg cggcagataa tcaaccagca gtagacatcc atgtattaca
     9061 aggtgaacgc ccaatggctg ctgacaacaa aactctagga agattccaat tgacagatat
     9121 tccagcagct cctcgtggta ttccacaaat cgaagtaaca ttcgatattg ataaaaacgg
     9181 gattgtaaat gtatctgcga aagatctagg tacacaaaaa gaacaaaaaa tcacgatcaa
     9241 atcttcttca ggtctaacag acgaagaaat cgaacgtatg gtaaaagatg cagaagcaaa
     9301 tgcagaagca gataaagctc gtaaagagga agttgaccta cgtaatgatg tagatgcatt
     9361 gctattctct gtggacaaaa cattgaaaga attagaaggc aaagtggacg aagaagaagt
     9421 gaaaaaagca gaaacagctc gcgatgaatt gaaagccgct gttgaagcaa acaacatcga
     9481 agaaatgaaa actaaacgcg atgcattgaa cgaaatcgtt caaaacttga ctgttaaact
     9541 atacgaacaa gcggcacaac aacaagctca agaaaaccca gaagcagctc aaggcggagc
     9601 agatgatgtc gttgatgctg attttgaaga agtcgatggc gacgataaat aatcagttca
     9661 ctgaatttcg agagaaggcc aaagttgcta ctttggcttt ttcttttgaa aaatagtttg
     9721 aagtatcggt ttttgtttca aacaaaatat gatatgataa caagcgacgt gaaaatgatg
     9781 agaaccaata ggaggaaagc ctatggcaac aaaacgtgat tattatgaag tcttagggct
     9841 ctcaaaagga gcatcagaag atgaaataaa gaaagcttat cgtaagcttt cgaaaaaata
     9901 ccatcctgac atcaataaag aacctgatgc ggaagaaaaa ttcaaagaag tatcagaagc
     9961 atatgagatt ttaagtgacc cgcaaaaacg cgctgcatat gaccaatacg ggcatgcagg
    10021 cacagatcca aattacggtg ctggtggagc tggtggcgga tttggtggat tcggcggctt
    10081 ctctggtggc ggcttcggtg gttttgaaga tatcttcgac tctttctttg gaggaggcgg
    10141 tcggtcagtc gatccaaatg cgcctcgtca aggtgctgat ttgcaatata cggtcgactt
    10201 gtcttttgaa gaagcgattt ttggtgtaga aaaagaaatc aagtataatc gggaagaaat
    10261 ctgtcatact tgtggtggga acggtgcaaa accaggcaca caaccaacta cttgtcataa
    10321 atgtcatggt gcaggtacga ttaatgtaga acgccaaaca ccacttggac gagtgatgag
    10381 ccgtcaaact tgtgatgttt gtcatgggac agggaaagaa atcaaagaac cttgtccaac
    10441 ctgtcatggc tcaggacatg agaagaaagc acattcagtt aaagtgaatg taccagctgg
    10501 tgtagaagaa ggacaacaaa tgcgtttagc tggtcaagga gaagctggag aaaacggcgg
    10561 accatttggt gatttgtatg tggtcttccg tgtagaagaa agtgatatct ttgatcgtga
    10621 aggttctgaa atttactatg aattgccttt aagttttgta caagcagctt tgggggatga
    10681 agtccaagta ccaactgtcc atggagatgt gaaattgaag atccctgctg gaacacagac
    10741 aggtacgaac ttccgtttac gtggaaaagg cgctccgaag ttacgtggtg gtgcaacagg
    10801 cgaccaacat gtcaaagtaa aactgatcac accgaaaaat ctaaatgaac aacaaagaga
    10861 agcattacgt gcattcgctg aagctggcgg catcaaagtt gaagaacagc aagaagacgg
    10921 tttcttcgat aagtttaaag atgcttttgg cgggaaaaag cggaagtagt ttttctagta
    10981 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatatgttc gcctggtttt
    11041 tttgtagttg tcatgtttgt accaacaaaa aagccagctt tagaatatga taatcgtcat
    11101 agtttctagg ggtataacca gtgagagaat ggaacttgtt caatcgatat tgcaaggtgt
    11161 ttttatgaat aaataattct tctgagcaat ttttgatgct tccgttattt ttactgaagc
    11221 atgccaaagt cttcttgaac aaatgtactt cttcttctgt caggcttttc aaaatacgtt
    11281 ttacatataa tgttcgttga tctgacggga ttgctggtaa aacgatcccg aggtctaaat
    11341 cttcaaaaaa gcggatctcg ttcacagatt gatggaaagc tatccattcg caaacttgtt
    11401 tggcatgaag gaaagattgc ggtgtctctt gctctgtata acaaagcgac ccaattccaa
    11461 agtttagctg taactgatac attttattaa actttgtctg gattttatca agcaatgata
    11521 gcaatgaggc tctggaaatc cgattgacta ggataatgat ttgttgtcta tgtacagtga
    11581 gcaaagcctg tccgtaataa gagaagaccc tttcgagcaa ctccaataaa ggattgatcc
    11641 gttcttgaca caattgctga ttgtcagaaa gttgtcccac aactacataa tatggagaag
    11701 aaaaatcaag ctcaattaag tgagggtggt atcgactatg tacttgatat catcaatgaa
    11761 ccgatggtac tttcttatgc gatggaacag gtagagcgat tgcttgtttg tgcaggaaaa
    11821 caagcgatgc ggctgatacg attaggtgag aaaataagaa gataaaaata cacggtccct
    11881 tcatggcgac cgtgcatttt tggtttttat taaatattta atactttatt caagaaatct
    11941 tttgtccgtt cattttgcgg attttcaaag atttgttgag gcgttccatc ttctaaaaag
    12001 tttccgtcgt caatgaacat gacgcgattg gccacttctt tagcgaaccc catttcgtgc
    12061 gtaacgataa ccatcgtcat accttgttct gctaactttt tcatgacgtt caagacatca
    12121 ccgaccattt caggatcgag tgcagaagta ggctcatcaa aaagcatgat gtcagggttc
    12181 atcgctagag cacgagcaat agccacacgc tgtttttgtc caccagaaag catttcagga
    12241 tacatatctt ttttatcttc tagaccaact gtctgcagta atttgattgc ttttttctct
    12301 gcttcttctc tagattcttt cttcaggtcc atcggtgcta aaatgatgtt ttccaaaata
    12361 gaaagatgag ggaataggtt aaagtgttgg aataccattc cgatatgttg tctcaccaag
    12421 ttgatatctg tgcttttatc catcaagtgg gcgccgtcaa taataatttc accgtcagaa
    12481 ggttcttcta aacgattcaa gcaacgcaag aatgtacttt ttcctgaacc tgaagggcca
    12541 atgatacaga cgacatctcc ttcgttgatg gatacattga tgtcgtttaa tactgtgtta
    12601 tcgccgtatt ttttgactaa atgctcaact aagatttttt ctgccatctt acttcacctt
    12661 cttttctaaa acttttgcta attttgtcaa tgctgtgatc aaaatcaagt acatgatcgc
    12721 aatgatcaag taaacatacg tactttgtgt tgtacgtgca acaatgatct taccagtttg
    12781 cagcaattcg actacaccga ttgcggaaat gatcgttgtg tcttttaatg agataacgaa
    12841 ctgattgacg aatgatggga tcatgatgcg tatcgcttgc ggcaaaatga ttttttgcat
    12901 tgtccgatta taagaaagac ctaaacttct ggaagcttcc atttgtccga caggaacggc
    12961 gttgatcccg cctcgaacaa tctctgcgat ataagcactt gcgttaagag tcaaggtgat
    13021 aacaccagca aggaagtcag gaatcgtaaa tccaacgata ccaggcaacc caaagaaaat
    13081 aaagaacgca agaaccatca ttggaattcc tcggatgata tcgacataaa tactagcaat
    13141 tgtgcgaaga gcgcgtattg gtgctacact gaataatcca aaaatcacac cgattactat
    13201 agccaaagca aaggagatca aagttaggag aattgttctc caaagtcctt tcaacaatgc
    13261 ttgatagttg ttttggataa gtccaagaaa tgttgattca tcagcagtgg aagactcact
    13321 ggaagtttct gaaccatccg ctacatattc tttaataatc ttatcgtatt cgcctgttct
    13381 ttttaattct ttcaatcctt cattgaacat attaagaagc tcaggatttt gtcctttttt
    13441 aacggcaaat gcatattttc cgccagattc ttttttgatc ggtgtttcca attcttgtcc
    13501 ttgtttgatc gcataaccga ttaccggata atcatccatc attgcatcga ttgcgccaac
    13561 ttttaacccg tcgtacaatt gatcagcagc atcaaaatat ttgatagaat agccatattc
    13621 agattcatgg tcgcttaaaa attcagcact ttcggtacca acttttgctc cgactgtttt
    13681 tcctttcaaa tcttcataag acttgatcga agtatctcct tttttgacag ctaattggat
    13741 cccgctatca aagtaagaat cagaaaaatc atagcttttc tttcgttcat ctgttacagt
    13801 cattccggca atcattccgt ctgcttgtcc tgattccaaa gcttgcaaag cagcagagaa
    13861 accacggtag tccattcgga tgttaaatcc ttgcagtttg gcgatgtttt ccatcaattt
    13921 tacatcgatg ccttcgtact cgccctttgc gttttgaaat tcaaatggag caaaagcaga
    13981 gtcactggca attacgtata cgtctttttt gggctctatt ttttgcattg tctcgcttgt
    14041 gcttgactct gccgttgtag tgttgtcagc ttgtgcaaca tgaccaaacg caaatgtcac
    14101 aagtcctgtc attaagaaaa caaatgagtg aaataaatgc tttcttttca taatcaacct
    14161 cctaaatttc caatatttta ttattatagg cgaaaagtgc tgaatcagac aagactgtaa
    14221 gctcgaaaaa atgaaaaaaa tgcgagtttc taacatttta tgttataaaa cataacatgt
    14281 ataataagat aaaaaagtgg cgaaagtatg ttcggtgtgg tataattttt tagtaaggaa
    14341 tgcctttgct ttccttttct ttttattggt cgtaaaatga aaaggaatga atgaaaagga
    14401 ggacgaagaa tgattgagag agatacctcc cgacactttg agcttgtctc gaaataccaa
    14461 ccggctggtg atcagcctga agcgattaac caattagtgg atggcgtagt cggcgggaaa
    14521 aaagcacaaa tcttattagg tgcgactgga acagggaaaa catatacgat ttccaatctt
    14581 attgagaaag tgaataaacc aacactgatt attgcccata acaaaacatt ggctggtcag
    14641 ctgtacgggg aattcaagga atttttccct aacaatgctg ttgaatattt tgtttcttat
    14701 tatgattatt atcagcctga agcttatgtg ccttcaagcg atacgtatat cgaaaaagat
    14761 tcaagtgtaa atgatgagat cgacaaatta cgacattcgg ctactagttc attattagaa
    14821 agaaatgatg tgatcgtcat tgcatccgtt tcatgtattt ttggattagg ctcgcctttt
    14881 gagtaccaaa aacaagtagt ttcgattcga cagggagcag aactagatcg caatcagttg
    14941 attcgtgact tagtcagtat ccaattcgaa agaaatgata tagattttca gcgcggtcgt
    15001 tttcgagtgc gcggtgatgt cgtggagatt tttccggctt caagagatga acgtgcctta
    15061 cgtgtggagt tttttggaga tgaggtcgaa cgtatccgag aagtcaatgc actgacaggt
    15121 gaagttttag gagaaacgga acacgtagca atcttcccgg cgactcactt tgtgacgaat
    15181 gatgaacata tggagcatgc cgtagcgaat atcaaagcgg aattagaaca gcgtttaact
    15241 gtcttgcgaa atgaaaacaa acttctggaa gctcaacgtt tagaacagcg aaccaattac
    15301 gatattgaaa tgatgttaga aatgggctac acgtcgggaa ttgaaaatta ttctcggcat
    15361 atggacggac gtaaagaagg tgaaccgcct tatacgctat tggatttctt tcctgaagat
    15421 tttttgatcg tggcggacga atcgcacgta accatgcctc aaatcagagg aatgtataat
    15481 ggtgaccgag cgagaaaaca gatgctagta gattacggtt tccgtttgcc atctgcttta
    15541 gacaatcggc cacttcggtt agaagagttt gaaaaacatg tcaatcagat catttatgtc
    15601 tctgcgacgc caggaccata tgaacatgaa caaacagata ccgttatcca acaaatcatc
    15661 cgtccgacag ggctgcttga cccagtgatc gaagttcgtc caatcatggg acaaatcgat
    15721 gatctagtag gcgaaataaa tgaacgagtc gaaaaagacc agcgggtgtt tgtaacgaca
    15781 ttgacgaaaa aaatggcaga agacctcaca gattatttca aagaattagg gatcaaagtc
    15841 aaatatttgc atagtgatat caaaacgctg gaacgaacag aaatcattcg agatcttcga
    15901 ttaggtgaat ttgatgtgtt agttggaatc aatttgcttc gtgaaggatt agacgtacct
    15961 gaagtttccc ttgttgctat cttggatgcg gataaagaag gctttttacg tagtgaacgt
    16021 tccttagtcc aaacgattgg acgagctgct cggaatgaag aaggtaaagt aatcatgtac
    16081 gcagacaaag taacagattc tatgcgtcta gcgatggatg aaacgtcaag acgtcgtacg
    16141 attcagcaga agtacaatga agaacatggg atcgttccaa aaacgatcat taaagaaatc
    16201 cgagatttga tttctattac gaaagaatcg gaagatgata caaaagaagc ggtacaagtc
    16261 tcgtacgaag aaatgacgaa ggaagaaaaa gacacgttat tgatgaaatt agaaaaagaa
    16321 atgaaagatg cggcaaaagc tttggatttt gaaacagccg cgaatgtgcg agatatgatt
    16381 ttagaattaa aagcttcaaa gtaggaggac aacatggcta acgataaaat tacgattcat
    16441 ggtgcacgtg cccataactt gaaaaatata gatgtaacga ttccacgaga taaattcgtg
    16501 gtcgttacag gtttgtccgg ttctggaaaa agttccctag catttgatac gctgtacgct
    16561 gaagggcagc ggagatacgt agagagcctt tctgcttatg ctcgtcagtt tttaggacaa
    16621 atggataagc cggacgtaga tagcatcgac ggattgagtc cggcgatttc tattgatcag
    16681 aaaacaacaa gtaaaaatcc tcggtcgaca gtaggtactg tgacggaaat caatgactat
    16741 ttacgcttgt tgtttgcacg tgtaggacat ccgatttgtc ccaacgatca tatcgagatt
    16801 accagtcaat cagtagatca aatggcagat caagtaatgg ctttgcctga gcgcacaaag
    16861 atccaagtgt tggcaccgat cgtggtaaag aaaaaaggac aacataaaaa aatattcgaa
    16921 cgaatccaaa aagagggata cgtccgcgtt cgagtcgatg gtgagatcta tgatttaagt
    16981 gaagtgcctg agctggagaa aaacaaaaaa catgatatcg cgatcgtcat cgaccggatc
    17041 attgtgaaag aagggattcg ttctcgccta tttgattctt tagaggcggc cctgcgatta
    17101 gcagacggct atgctctggt tgacgtgatc ggtgaagaag aaatgttgtt tagcgaacac
    17161 tatgcctgtc cttattgcgg atttactgtt ggcgaactag aaccaagact gttctcattc
    17221 aatgcgcctt ttggagcttg tccagactgt gatggtttag gtgtcaaatt agaagtcgat
    17281 caagatttag tgattccaga tagaacgaag acacttagtc aaggtgctat cgttccatgg
    17341 aatccaatca gctctcagta ttatcctcaa atgttagcgc aggcttgtga aagtttcggt
    17401 atcgatttag atacaccatt cgaagaatta ccggaagagc atcaggagat catcttgaat
    17461 gggacagatg atcttttcca tttccattat gaaaatgatt ttggtggcgt tcgcgatgta
    17521 gaaacgactt tcgaaggggt attgaagaat atcgagcgtc gttatcatga tacgaacagt
    17581 gatttcacgc gtgagcaaat gcgtctttat atgacagaat taacttgtca gacatgtaaa
    17641 ggttatcgat tgaatcccca agctctatct gttcaaatca atggaacgaa tatcgggcaa
    17701 acaaatgagt tgtctattca aaaagcagta gatttcttct caaacttaac gttatctgaa
    17761 caagagaaag taattgccaa accgatttta aaagaagtaa atgacagact ttcattcttg
    17821 gaaaatgtgg gactagacta tctaactttg agtcgcgctt ccggaacatt atctggggga
    17881 gaagcgcagc gaatcagatt ggctacacaa attggatcga atctttctgg agttttgtat
    17941 attttagatg aaccatcgat cggattgcat caacgcgaca acgatcgact gattggctca
    18001 ttaaagaaaa tgcgtgatct tggcaacacc ctcgttgtag tcgaacatga tgaagatacg
    18061 atgtgggcag cagattattt gattgacgtc ggacctgggg ccggagaaca aggcggacaa
    18121 atcgtggctg caggcactcc tgaagaagta gctaatgatg aaaattcttt gacagggaaa
    18181 tatctttcag ggaaaaaatc aatccctgta ccaaaagaac gccgtaaagg taatgggaaa
    18241 gcaatcaaga tcactggtgc ttcagaaaac aatctgaaaa atattagtgt tgaatttcca
    18301 ttaggagaat ttgtagcagt cacaggtgtg tctggttctg gaaagagtac attggtcaat
    18361 agtatcttaa agaaatcgtt agcgcaaaaa ttaaataaga attctgctaa gccaggtaaa
    18421 ttcaagacaa tttccggcta cgaaagtatc gaaaagatca tcgatatcga tcaaagccca
    18481 atcggccgga cgccgagaag taatccagcg acttatacaa gtgtatttga tgatatccgt
    18541 gggttatttg ctcaaacgaa cgaggcaaaa atgcggggtt ataagaaagg gcgttttagt
    18601 ttcaacgtaa aaggcggtcg ttgtgaagct tgtcgcgggg atggaattat taagatcgaa
    18661 atgcactttt tgcctgatgt ctatgttcct tgtgaagtat gtcatggtaa acgatataat
    18721 tctgaaacat tagaagtgca ttacaaagga aaaagcattg ctgatatttt ggaaatgaca
    18781 gtagaggatg ctgtagaatt cttcaagcac attccaaaga ttcatcgcaa actgcaaacg
    18841 attgttgatg ttggcttagg ttatgtgacg atggggcaac cagcaacgac attgtccggt
    18901 ggtgaggcac aacggatgaa acttgccagt gaattgcaca aaatctctaa tggaaagaat
    18961 ttctatatac tagatgaacc aacgacagga cttcatagcg atgacatcgc ccgcttgttg
    19021 catgtattac aaagattagt agatgctggt aacacagttt tagtgattga acacaatcta
    19081 gatgtaatca aaacagcaga ttatatcatt gatttaggac cagaaggtgg agaaggtgga
    19141 ggaacgatcc ttgcgactgg aacaccagaa gaaatcatta acgtaaaaga aagttataca
    19201 ggtcactatt tgaaaaaaat aatggtataa agaggataga tagctgagat agttaaaaaa
    19261 acataaaaaa atgaattttt gcttgtttta ttaatatcag tgttctagaa tcattacatg
    19321 gtagttcttt ctacactgat tggtatagat aaaaacttcc tataagttca atccgttcaa
    19381 aaaagaattg agcaatcgga ggactttcct ttgattgctt ttttctttat ggtatgatat
    19441 tccttgtgta gttggaaaag aaaaacaaga gattatgagt aagtgagaga taatgatgaa
    19501 cagtattgaa aatgaagaat ttatccctat attattagga agcgacatga acgtttatgg
    19561 tatggcacgt tcttttaatg aagcatacgg caaaatctgc caagcatatg cttcggatca
    19621 gttagcccct acccgctaca gtaaaattgt caatgtagaa gttattccag gatttgataa
    19681 agaccctgta tttatcgaga cgatgcttcg tttggcaaaa gaaagatatt ctgataagag
    19741 taaaaaatat cttttgattg cttgtggtga tggatatgca gaattgatct cccaacataa
    19801 acaagagctg tcagaatatt ttatctgtcc atatattgat tattctttat ttgaaaggct
    19861 gatcaataaa gtaagctttt atgaagtttg tgaagaatat gatcttccgt atcctaaaac
    19921 attgatcgtc agagaagaaa tgttagtaaa tggacatttg gaacaggaat tgccttttga
    19981 attcccagtt gctttgaaac ctgcaaacag tgtggagtat ctttctgtcc aatttgaagg
    20041 aagaaaaaaa gcttttattt tagagactag agaagagttc gatttaattt taggccggat
    20101 atacgaagcc ggttataaaa gtgaaatgat tgtacaagat tttatcccgg gtgatgatag
    20161 taatatgcgt gtgctaaatg cgtatgtaga tgaagatcat caagttcgga tgatgtgcct
    20221 tggtcatcca ctattagaag atcctacacc agcatcgatt ggtaattacg ttgtgatcat
    20281 gccagattat aacgaaaaaa tctatcaaac cattaaggca tttttggaaa aaatcgaata
    20341 tacaggcttt gccaatttcg atatgaagta tgatcctaga gatggagaat ataaattatt
    20401 tgaaatcaat ttgagacaag gtcgtagcag tttctttgtg acattgaacg gcttgaatct
    20461 ggctcgtttt gtcacagaag atcgagtgtt caacaaaccc tttgttgaaa cgacctatgg
    20521 aacgaatcaa tcagataaag caagattatg gatgggtgtg cccaaaaaaa tctttttaga
    20581 atatgctcgg gaaaatgaag acaaaaaact agctgagcaa atgatcaaag aaaatcgtta
    20641 cggtactact gtattttatg aaaaagaccg atcaatcaag cgctggttgt tgatgaaata
    20701 tatgttccat aactatattc caagattcaa aaagtatttt catgtgaaag aaggctagtt
    20761 catggagaat tttttcagta ttttaggcgg aatgggcacg atggcgacag aaagttttgt
    20821 tcgcttgatc aaccaccgaa cgaaagctac aaaagatcaa gaatatttga attatgtctt
    20881 attcaatcat gcaacagttc ctgatcgtac agcttatatt ttagatcgat cagaagaaaa
    20941 tccaatgccg tttctgctgg atgatattga gaaacaaaat ttattgcgac caaacttcat
    21001 tgtgttgact tgtaacaccg cacactactt ttttgaagag ttacaagcgg caacggatat
    21061 tcctatcttg catatgccaa gagaagcagc aaatgaactt gtccgtcagc atacgacagg
    21121 gagagtagcg attctaggaa cagaaggaag tatgaaagct ggaatctatg aacgcgaagt
    21181 caaaaatctt ggtttcgaaa cagtgattcc tgatactgcg cttcaagaaa aaatcaatta
    21241 cctgatctat catgagatca aggaatcgga ttatttgaat caagagctct attacgaaat
    21301 cttagaggaa gcggtagagc gcctaaactg tgaaaaagtc atcctaggtt gcacagaatt
    21361 atctttgatg catgaatttg ctgaagataa tcattaccca gtgatcgatg ctcaatctat
    21421 tttagcggat cgaacgatcg aacgtgcatt agctgaaaga agcgaggcat tggatacagc
    21481 atcggaaaag taaaaaaatt ggatttgggg caaatatcct aaaagatatt tggtccaaat
    21541 ctttttgtaa aagaaaaaga aaactggtaa tgatacgccc gggatgatat aattttatta
    21601 tgcacaatgt gagaggggtt cgtaaatatg acagataatt tgcaattagt catcataaca
    21661 ggtatgagtg gtgcagggaa aacagtagct atccaaagtt ttgaagatat gggctatttt
    21721 tgtatcgata atatgccgcc tcgattgatt ccaaaattct gggaattgat caaagaatca
    21781 ggtaaagtca caaaaatggc tttaggagtg gatttgcgtt ctcgttcctt ctttgaagaa
    21841 atccaaaaca tgctgatcga aatcgaaaat acgaacttca tcgacacttc tatcttattt
    21901 ttagatgctt cagacgaaga actagtggct cgctacaaag aaacaagacg agcacatcca
    21961 atggcgatgg acggtttgat cactgaaggg atacgtaaag aacgggcgat actagacgac
    22021 ttgaaagccc aagcttccgt gatcattgat acgacaacat tgacaccacg acaattacgt
    22081 gaaaaaatca atgagtcatt caaatcaaag ggagaccaag gatttcggat cgaatttgta
    22141 tcatttggtt ttaaatacgg gttgccgatc gattcggata ttgtgatgga tgtgcgtttt
    22201 ttgcccaacc ctcattatat cccggaacta agaccattga ccggaaaaga tcagccagtc
    22261 tatgattatg tgatgtcctt tgatgaaacg caatcattct accataaatt tttggatctt
    22321 ctggaaactg tattgcctgg atacattaaa gaaggaaaaa gcagcttgac agttgcaatc
    22381 ggctgtacag gcggtcaaca ccgttctgtg gcattgactg aacgtttggc aaatgcttta
    22441 gggaaaaact ataaagtcaa tgtgacacat cgggacaagg ataaaaggaa agagacggtg
    22501 aaccggtcgt gaaaatgaaa acctaccgga taagaaaacc aaagatcgta gtggtcggcg
    22561 gtggcactgg tctgccagtt atcctgaaaa gtttacgaaa tcaaggagca gatatcacag
    22621 cagtagtgac tgttgcagat gatggtggaa gcagcggtgc aatcagagaa tcgatcgcta
    22681 tggctcctcc tggtgacttg agaaatgtct tagtggcgtt atcggatatg ccacaattct
    22741 atgaagatat tttccaatac cgtttcaaaa aggaagatca gtttttagcc aatcatacga
    22801 tcggtaatct gatcattgcc gcagtttccg agatgcgggg aagcacttat gaggcgattc
    22861 aattattagc aaaaatgatg catgtagatg gacatgttta tccttcttca gaaactcctt
    22921 tgaccttgca tgctgttttt aaagacggaa cgactgcagt aggagaatct aaaatcgcga
    22981 tcaaccgtaa aaccattgac cgtgtgtatg tctgcaacac aaatgataaa agtgaagcca
    23041 aagcggctcg aaaagtcgtt tcagcaatta tggacgccga catggtcgtt ttgggacccg
    23101 gaagtttatt caccagtatc cttccaaatt tagtgatacc agaaatcggc gaagcaatgc
    23161 tcaagacaac tgctgaaacc gtctatatct gtaatatcat gacccaaaaa ggggaaacgg
    23221 aacatttcac agatgccgat catatacgtg ttttacacaa gcatttgaat cagcctttta
    23281 tcgatacagt attggtcaat acggaaaaag tgcctgaaga ttatatggac ccagagatct
    23341 atgatgagta tctagtacaa gtcgcacacg acttcaacgg attgagaaat gaaggatgtc
    23401 gagtgatttc caccgacttt ttagaattga aagatggtgg tgtcttccac gatggagaca
    23461 aagtagtaga agaattgttc cgtttagttt tcggttcaaa atattaagca atttattgtt
    23521 ggagaaagga ggagaatagg atgtcatttg ctgcagatgt gaagaaagaa ctaactggtt
    23581 tggaagttca tcgagaacat gcgaaagcgg aattagctgc tttgctgcga atgaacggct
    23641 ctttaggtat tgtgaaccag cagtttattt tgaacgtcca aacagaaaat gctgcaattg
    23701 ccagacgtat ctattctctt ttgaaagatc attatcaggt acgaagtgag ctactcgttc
    23761 gtaaaaaaat gaaattaaaa aagaacaatg tctatatcgt ccggttgaag caaggcacaa
    23821 aagatgtgtt gatggatctt gatatcatgg atggtatgat gttcaatgga cacgtttcgg
    23881 acgagattat gggaaattcg caaaaaatgc gttcttactt acgtggtgct tttatggcct
    23941 caggttcagt caataatccg gaaacgagtc gttatcattt ggaaatattt tctatttatg
    24001 aacagcataa ccaagacatt tgcgatatgt taaattatta cggtctacat gctcgaacgc
    24061 ttgaacggcg caatggctat atttcgtatt taaaaggagc agaaaaaatt gctgatttct
    24121 taacattgat tggtgcaacc aactcaatgc tccgatttga agatgttcga atcgtacggg
    24181 atatgagaaa ttcggtcaat cgtctagtca attgtgagac agcaaatctg aacaagacga
    24241 ttgatgcggc ttctaaacaa atcgaaaaca tcgaattgat cgaagcaaag gtcggtctac
    24301 atgcccttcc tgaaaaactt caggaaatcg cagaacttcg gcttcaacat ccagaggtca
    24361 gcttgaaaga attaggggaa atgattccct caggtgcaat atcaaaatcg ggaattaatc
    24421 accgaattcg caaaataaat gaatttgcgg aaaaactgcg tgaagaagca gtttaatcca
    24481 aagaaaaaac tgatacatga agcaggagat gcgaatgtat cagttttttt taaaagctat
    24541 acttgctttt cttccgtcaa actataattc caactttact ggagtaacat aatgttgaaa
    24601 ggcttctatt gttttttaat acaatcgtat aaaaagcggg gagacaactg aatgaagatt
    24661 ttagttttat acataaactt ttgcttaata tagaagtcct gatatagata ggatcaagta
    24721 tttatctata actggagatt aattaaaaac tggaaggaac tgctcgatca accttgagaa
    24781 ctgtttttct tacttctggt caagattctc gtggttggtg gataaggatg gttgataagt
    24841 tcattggatt agctagtcga aaacaaccag gtgtaattaa tgtagaagtt ttaacaaggg
    24901 gatggagtga tttattcagt gataatcata agttcataaa tagtaattgg gatgtatggt
    24961 tggtatacgt tatacctgaa cttgaactat caagatttgg tttagaattt gagaatcgat
    25021 gtttataaag ggagggcaaa aaatgtgctt ttttacaact tttactatca ttatgattgc
    25081 agtacttttt atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt
    25141 tttacttcga gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt
    25201 tattgtattt atttggagaa ctacagcacc accattgcca gagattacat atggagaatt
    25261 tccgtttagt cttgaatatg aaatagatga ggaaagaatt ttgattgaag atacggtgat
    25321 tgcagaattt ataggaagtt ttagagggaa tgtgacaact caacctagac gaagttggag
    25381 tataaaactt ttaaataata gtggagagtt gaaagcattt cattatgaat tcttaataat
    25441 agaaagtgaa gatgtaacaa tcactttaag tagtggctct gctggatatt ttatgaatga
    25501 tggtggaagc aagccacagc tcacagtccg tgatcgtatt gcaggtttta tgcatttttc
    25561 tcactatgac ttagaagaag ctcgtaaaat acttgcagaa tatgggatca cattaatcaa
    25621 ttgggaatat gcgccaccga taagaaattt tttcaatgat tattattagt cttataaaaa
    25681 acagaatgta aaatttatgt gcgcgagtat ttagttttcc attttttgtt tgatagatat
    25741 atagcattgt tgaagcatgg gatagtggtg ccaattttat caatgaggtt ggtcaggcag
    25801 ttaacataat tggtgatgct ataaatccat ttaattggaa ttggaggaat taggtgtgga
    25861 aacagtgaga ttatggatat tcatgaaaaa ttttcgttcg gctttgagat atgttattct
    25921 ggaatcaaaa tcaggttatt atctgattga cacagatcac tatacttttt ggggaggcta
    25981 cctttttccg atttttaatt ggataatccc ccataaggtt atgaaaatta acttgcctgc
    26041 agaagaaata aatagattaa tactcaacaa agatttacaa aggaaaagag agaaagaaca
    26101 ttctaatgtt gcttggatag gtccagcatt tgcagtagtg acttcttcaa ttgtcggtcc
    26161 tatgctagag agtatttata gtgatttttc tattggaata cgttatttat ttgttacttt
    26221 aagtatagct gttcttttgt gcataaaggg ttatgttagc aagaagaata aagatatttt
    26281 aacactaatc ggtgtaaaca atttatctag tactaaaatt atactgtttc ctgaatctat
    26341 aaaagaagga tcaaggttgt tgataatttt tataatgctt atactgtttt ctgttttatt
    26401 tggggtgatg tttataga
//
LOCUS       DADOOS010000025        26375 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.025, whole genome shotgun sequence.
ACCESSION   DADOOS010000025 DADOOS010000000
VERSION     DADOOS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.025"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            112..408
                     /locus_tag="J8C37_000891"
     CDS             112..408
                     /locus_tag="J8C37_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAZ5304350.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(931..1884)
                     /locus_tag="J8C37_000892"
     CDS             complement(931..1884)
                     /locus_tag="J8C37_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5304351.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1907..2662)
                     /locus_tag="J8C37_000893"
     CDS             complement(1907..2662)
                     /locus_tag="J8C37_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5304352.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2659..3618)
                     /locus_tag="J8C37_000894"
     CDS             complement(2659..3618)
                     /locus_tag="J8C37_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAZ5304353.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3611..4561)
                     /locus_tag="J8C37_000895"
     CDS             complement(3611..4561)
                     /locus_tag="J8C37_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAZ5304354.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4711..5163
                     /locus_tag="J8C37_000896"
     CDS             4711..5163
                     /locus_tag="J8C37_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAZ5304355.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5252..7519)
                     /gene="gshAB"
                     /locus_tag="J8C37_000897"
     CDS             complement(5252..7519)
                     /gene="gshAB"
                     /locus_tag="J8C37_000897"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAZ5304356.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7706..8704)
                     /gene="ftsY"
                     /locus_tag="J8C37_000898"
     CDS             complement(7706..8704)
                     /gene="ftsY"
                     /locus_tag="J8C37_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAZ5304357.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8715..9524)
                     /locus_tag="J8C37_000899"
     CDS             complement(8715..9524)
                     /locus_tag="J8C37_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5304358.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9533..13114)
                     /gene="smc"
                     /locus_tag="J8C37_000900"
     CDS             complement(9533..13114)
                     /gene="smc"
                     /locus_tag="J8C37_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAZ5304359.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13134..13820)
                     /locus_tag="J8C37_000901"
     CDS             complement(13134..13820)
                     /locus_tag="J8C37_000901"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAZ5304360.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14168..15949)
                     /locus_tag="J8C37_000902"
     CDS             complement(14168..15949)
                     /locus_tag="J8C37_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5304361.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15975..16889)
                     /locus_tag="J8C37_000903"
     CDS             complement(15975..16889)
                     /locus_tag="J8C37_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304362.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16916..17878)
                     /locus_tag="J8C37_000904"
     CDS             complement(16916..17878)
                     /locus_tag="J8C37_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304363.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17878..18822)
                     /locus_tag="J8C37_000905"
     CDS             complement(17878..18822)
                     /locus_tag="J8C37_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304364.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18823..19827)
                     /locus_tag="J8C37_000906"
     CDS             complement(18823..19827)
                     /locus_tag="J8C37_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304365.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20095..20337)
                     /gene="acpP"
                     /locus_tag="J8C37_000907"
     CDS             complement(20095..20337)
                     /gene="acpP"
                     /locus_tag="J8C37_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAZ5304366.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20459..21460)
                     /gene="plsX"
                     /locus_tag="J8C37_000908"
     CDS             complement(20459..21460)
                     /gene="plsX"
                     /locus_tag="J8C37_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAZ5304367.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21509..23545)
                     /gene="recG"
                     /locus_tag="J8C37_000909"
     CDS             complement(21509..23545)
                     /gene="recG"
                     /locus_tag="J8C37_000909"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAZ5304368.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23662..25344)
                     /locus_tag="J8C37_000910"
     CDS             complement(23662..25344)
                     /locus_tag="J8C37_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAZ5304369.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25396..25758)
                     /locus_tag="J8C37_000911"
     CDS             complement(25396..25758)
                     /locus_tag="J8C37_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ5304370.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26028..26216
                     /locus_tag="J8C37_000912"
     CDS             26028..26216
                     /locus_tag="J8C37_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAZ5304371.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7272 a         5721 c         4640 g         8742 t
ORIGIN      
        1 aaaaaacctt taacaactat tggaaaaaga attacttttt gttcacggat tattaacaac
       61 tcttcggtag agtaaactta tcaaataaaa gacttctttg gaggaatcac tatgaaggaa
      121 tatataaaaa acatctattt tatcgaagaa acacaaaata tcgagggttc ttatattgaa
      181 gtaaaaacac tctttgtcaa tgaagataaa accaaagcat tggatattta taaaaaatta
      241 gcttcaaaaa aaacgaatag ttttggattg atattaagcg agtataaaat caaagcagaa
      301 gagagttatt tctatcaatt attaaaacgg tggagtaagc ttcccgccga tttttatcga
      361 aaaatgcaga tcatcaacta tcaaccgctt gctgagacac atgcctaaat agaggttgtg
      421 aaatcagtgc tttgagctaa taccgaaggt cagctgattg aacattgttt agaagagatt
      481 gtgacaactg cgatttgctt tgagtaataa gaacgctgga cagaaaatag cttctcatat
      541 tttctagtct agtggactta ttgccgagaa gcagcagatg gaacatcgtt taaagaagag
      601 atcggtacaa aataaaaata tctaaattta agttttatct aagtttcgct ttgtaccttt
      661 gattcaaatc aaaggaggcg atcttgatgc tgaaaaaatg gatcacacta ttttttgcca
      721 ctccccattc tagaatcaac ccgtatgatg tagataatga attcacgttg aaaaaagatt
      781 tctcaaataa ataaaaacca tccgtaaaat catcaatacg attttacgga tggtttttga
      841 taacttcttt gaggataggc tcttgctatt taagaatgta agaaacatta ttgttacata
      901 gtatgagagt ttaatcctct gttatttatg ctatttaaat gcttgattca catcttcgat
      961 catcagtttc attgattcta aaccgccccc gcttaaatac cagacatctg gttcaaggga
     1021 gatgatctgc tgatttttac cagcattcgt ttgtgctacc agttcattgg cagaaatatc
     1081 atctttgctg tcgtctccac caatagcttt tgttctgtct actacaaata agatatccgg
     1141 atttttttcc agcacatatt cataagaaac actttgaccg tgagtggatg cttcgatttg
     1201 atcatctgct tgttcaaagc caaataaatc atggatgaaa ccgaatctag aaccagatcc
     1261 gtaggctgat aattgccctt cattcacaag tgtgactagt gcttttttgt cagtagcttc
     1321 tgctttcttt ttcgtctgat cgattgcact agataattcc tctaattgag ttttagcttc
     1381 ttcttgctta ccaaagatct cccctaaagc agtgacattt tgctggaaag attcccatgg
     1441 atttttcgca tctaaagaaa ggaaaatggt cggagcaatt tgacttagat ctttttgaaa
     1501 atctctttgt cggccagaaa tgatgattag atctggctga agctgattaa ttttttctaa
     1561 gtctggttct tttattccac ctgctgattc tactttttta taggatgaaa gatactcagg
     1621 aaggctgctt gtagctgctc caactacctt atctccaaca ccaagagcat ccatcgtatc
     1681 taaagaacca ttgtcgaata cgacgacttt ttctggatta tttggcacct cgatttggtc
     1741 tccattacta tctgttatcg tcaatgtagt ttcagtttgt gtgctgcttt tttttgtgtc
     1801 tgcctgactg gtgttgttgc tatttccaca tgctgctaaa ccaaatgatg ctagaatcaa
     1861 gatacctact actaattttt tcatctcttc tctcctaatg ttagttttag aaataaagac
     1921 agaatctgcg gccttcaatc tcacaaatcc gtatgtccat ttcgtataat tcatccaata
     1981 catctttttt tatcatttct tgggtcgttc cttgcgtaaa taactgtcca tctttcattg
     2041 caacaatttc gtctgcataa cttgcagcaa agttgatatc atgaagaaca ataacaattg
     2101 ttttcccgat ttcttgtact aaatttttta aaatctgcat gatttgaacc gcaaaattca
     2161 tatctagatt gttcaaaggt tcatccagta aaatataatc cgtgtcttgc gctagcacca
     2221 ttgcaatata cgcacgttgg agttgaccgc ctgaaagagt gtcaatcgat tgatccgcta
     2281 tttcttctaa cccaaggtgt tccagtgatt cctgaacgat ttcctcgtct tttgtcgtca
     2341 attttccttt cgtataagga aatcgtccaa attctactaa ttctttcacg gtgatttgga
     2401 catttatttc attcgattgt cgtaggatcg ataatttttt ggctaattca ttggatttcc
     2461 aagcctttac ttcgttatgg tctaaataga tttcacctgt atcttttggt atcaaacgac
     2521 tgatcatcga caacaccgtt gactttccag caccatttgg accgataaaa gcagtcaatt
     2581 tcttttcagg aatcgttaac tgtacatctt taaccactga ttttccatgg tacagtttat
     2641 tgaccttttg taaatcaatc ataggatact cttcctttcg cgaatcaatt ttgtgacaaa
     2701 ataaattcct ccgacaaatt caacgacgac actcactgtt gtattccagc cgaaaatctg
     2761 ctctactgcg agctgtcctc ctgtcagaaa cagcatagct accagaaagc ttccgataaa
     2821 tagctgacca tgacgataag tagaaaagac acgataactg atatttgcga caataaaccc
     2881 taaaaaagta actggaccca caagagccgt tgatgtccct gtcaaaagac tgacacaaca
     2941 taaaatcaaa aattcaaaac gatctgtgtc tatcccgagg tttactgctt gttcatttcc
     3001 gagatggaaa acgtctagca catgtcgata tcgaaataac aggtaccagc agccgataat
     3061 gatcaaagaa gcaggcaaaa gatactgact ctggatattg ccaaagcttg caaataatct
     3121 gccttgaagc aaatcatatt cattcggatc catgatgact tgtaaaaagg tactgacact
     3181 accaaaaagc gtacctaaaa tcatccctga catcagtaat aaaaaaagat tcgctctcat
     3241 ttttttcaaa agaaagaaaa ataacaatag actcagcccg accatcagcc caacgttcaa
     3301 taagaaaaaa actgtacctg tctgctgaag catcgtcact cctccgataa agaagaaaag
     3361 gaccgtttgg acaaggacat ataacgactc aagtcccaat actgaaggag tcaaaaactg
     3421 gttatgcgta attgtctgaa agctgattgt ggcactgctg acagctagcg agacaaggat
     3481 aaatgcaaaa attttcttac tcctcaatgc acatgcaaaa tgccagtttc catatgtatg
     3541 gatcgtcaag tacattcctg taacaaataa aacaagaaca agcataccaa taaaaataga
     3601 tggctttttt ttaagcatat cgtcgtcccc ttatcagaag ataaaggaag atagccccac
     3661 cgatgattcc aacgatcaca ctgactggta tttcataagg aaaaatcaac acgcgagcga
     3721 caagatcgca aaatactaag aacaaactac ctgctaaagc agttacgcca attgttttgt
     3781 gcacttgatc tccatatatt ctagagacaa gattcggaat gatcactccg ataaaaggaa
     3841 tgcttcctac catgatcaac atgactgcac tagccaatga tactaagcca atgcctaaaa
     3901 actgcatctt ctggtaatct attcccagat ttactgcaaa cgattcgccc agaccaatga
     3961 tcgtaaatcg gtaagcaaat agccaaacca agagcaacaa tggaacaaca gcatacaata
     4021 actcataatt tcctcgtatc acagtcgcaa aattcccttg aagccatgaa gtcatatttt
     4081 gtaccaactg gaattgataa gcaaaaaaag tagcaactga accaatgata ttccctaaca
     4141 tgactccaat caacggaagc atcaattgat ttttctggag gaacaatcgc gtaagggata
     4201 aaaaaagcaa tgttcctgca aaagcaaagc aaaatgctgt caaggatcgg acaatcaagg
     4261 atgcaccagg taggaaaatc atagccacta aaatacctaa acgcgcacta tccatcgtgc
     4321 ccgcagtcgt tggtgaaaca aatttatttt gggtcaaatg ttgcatgatc aacccacaaa
     4381 tactactggt cgctcccaca aggatcaaac aaatcgttcg tggcaaacga gtcactagca
     4441 agatttgtaa gctttgccca tctaactgga gcagttctga caattgtaca ttcgatactc
     4501 cgatgaacaa tgaaatacta cttaaaataa ctaataatgc tactagtcgt ccgtagcgca
     4561 tccaatccac ctttctaatt gagaatgatt ttcattctta ttttaagtga atcgttgaac
     4621 aaaagcaacc gctttttcat atgttttagg tattattggg aatatgctat aatcgtaaga
     4681 aagacaaaga gaagaaggag ggtatttttt atggcacaag atgatgtaaa aagccatttg
     4741 gataagggac tttatggtac acctttagtg aaccctgaag aacagcataa atatttaggg
     4801 acattcaggg aacgctgcta tcttagtatg actgttgctg aaatgatcca accaaaaaac
     4861 aaagaaaact ttttaaaaga actcaaaaaa catccagatg cttccatact cttgaatggg
     4921 gctctacccc tttctgtcca aaaccaatat atccagctag caacgaaaaa caatagccgt
     4981 ttcactgtag tcaacgatta cgtcactgca aatcctgagg aattcggatt gctcttaaca
     5041 gcaaaagaag cagtaaatga acctgtgatt gatatcgaaa aaaaataccc agaagaaaac
     5101 tcttcgccag ctgaaaaaga aaaaccaaaa aagaaaaaaa gcttctggga tcgtttattt
     5161 taaaacgaaa aaacacataa atttttacat gcaaaaaaag cttttgatca gattgacatc
     5221 aaataggaca atctgtcaaa agctttttct tttaaaatac ttcaggatat aacaatttca
     5281 atacattcat cgtcaatctt ctgccttttc ctgcaaaagg ataaacatgc atatgcatgg
     5341 ctggattgaa atttgcttcg atgatcccat atgctttttt atcagttgta ggatcgattt
     5401 ctttatctgg tataatcaaa tcgataccac taatcttagc tcctagtgct tctaccgcag
     5461 aaacggcgat ttttttgtaa gcttcagaaa attcatcagt catatcgatc gaatcaccac
     5521 ctgtactgat atttgagttc tcccttagat aaacaatttg attggcttgg ggaactgatt
     5581 cgatcgtcaa cccttgttct ttcaacatca attgttctaa ttcacctaat tggatcaatt
     5641 ctaaaggtgc acgatgattc gttccacgca aagggtcact atttttttct tcaacaagtt
     5701 ctttcactgt tcggatactg tcaccaataa cattagcagg gactcgcaac atgatggctt
     5761 ggacttctcc gtcgatcaca aagaaacgat attccgttcc cggcataaat tcttctacta
     5821 aaaccgaact gtcttcacga aaagcaatgg ctaaacctgc ttgataatct tccagactag
     5881 ctccttcctt gaaaatcgtg atacctaacc cgtaattggt tgatttaggt ttgatcacaa
     5941 atgcctgctc ggcaaatctc ggataagctt ttaccgcttc ttccatggat gaaaattctg
     6001 cgcctcctgg tacccggaac ccagcttctt ttaagacttt tttcgttact gttttgtttt
     6061 ccatgataag tgggacgata tagctgtcct tacttgtcat gtttgcattt ttcacatatt
     6121 ctacatggtc ttggaattgc agacgcaaaa attgatccga ctcatctaat actttgactt
     6181 ggacaccttt ttggatggca tcgaacataa atatctgagt agaaagttcc atttcgcgaa
     6241 aaccggccaa ctgataggga cgttcatggc tttctttata atattgatgt cccaatgcca
     6301 cagcaacttc ttgctgactc gatagctgtg cttcttgata gagtttcgct gctaacgttt
     6361 gttcaggatg atccatttga gtcaatgctt cctctactaa ataaagagaa tccaaccaat
     6421 ccaattcaac cagcatttgc ttcattcctt caagaatttc tctaccttct tgatgaaatt
     6481 ctgtttgatc tgatggttgt tctagtgcta ctttgttatt tcgagtcgta ccttctgcta
     6541 cccattggtc acagtcttct ttttcttctg tccataacat gtacatcagg aataattgta
     6601 agaaccgaac ttgctctgga ttgataccca atcttgcata aggattcaaa tcgatattcc
     6661 gcaattcaat ataacgaaca cctgttgttg ctaaatctgc gactttcttt ccgcctcgga
     6721 aacgaagcgg tgtataaaat tctttctcct ccgaaagttt cccttcttcg atcattcgct
     6781 cgatatctgc taaatactgt ttcatagaag cataggaaac tttcacattt ggatgattcg
     6841 tatagcccag cgcactgttg cgaatactgc gaacaggttc ttgcggatgt gattcatcta
     6901 aaaagtagtt cttctcactc attggtgaag caccgaataa ataagtgatc atccagcgat
     6961 agcgcataaa gtttctcgct gttttcaaat aaagttctgt tttgaattcg gaaaaatctt
     7021 gaaattcttc ctgatggctg aacaatgttc ttagcaactc atcgccaaat tcaaaattga
     7081 aatgaatacc gcttaccatc tgttttcgct tcccgtattc ttttgctaaa taacgtcgat
     7141 acaagacatc ttcgaaattt tttaattttg caataatgat ctcttcgtct ttttccggta
     7201 aggcaggtgg catacttaat ggccagatca tctcttcttt cggtatagaa cgagcagtca
     7261 catcataaac agcagcgaga tactgaaata attcgggaat agaatcggta acaggggtga
     7321 tcatctctgt ttgtgtttcg ctaaaatctg tttggatata ggggtgatag gatcgatcgc
     7381 caaagattgc tggatgatcg gtttttgcta gatttcctgc aagatcaacc cgctgtcctt
     7441 cccgttctat accaaatcga gcttggtcga taaaagggcg cgcattgaca tgcaataata
     7501 attgcttaaa attcatcatt aagtgacctc caattcatat gaatctaatt ataatcaaaa
     7561 agtctaagaa aaagaacgaa attgtttgag aaccgcttac aaataagtat aaaaatgaag
     7621 gcatcaaaaa cacagctatg ttaaaaacaa tcattcaaac aaggagctgc aacttttgtc
     7681 acagctcctg ctttttagac atgggttatt cttcttttag taatcctttg aacaatccaa
     7741 cgacaaaatc atttggatca aacggctcca agtcatcgat tccttcacca agaccgacca
     7801 atttcactgg taaatgcaat tcattacgga tagcaagtac gataccgccc tttgctgtac
     7861 catctaattt tgtcaaaaca agaccggtca catctgttgt ttctttgaat tgttttgctt
     7921 gaaccatcgc gttttgacca gtggttgcat ctaatacaag aagtgtttct tggggtgctt
     7981 ctggtaattc ccgctggatt actcgtttta ttttttctaa ttcgttcatc aagttcactt
     8041 tattttgcag tcggcctgca gtatcaacta gtaaaacatc tgcctgctct gtctttgctc
     8101 tttccaaagc atcgaatacg acagctgctg gatcgcctcc tgcattgcca cgaacaactt
     8161 caacaccagc gcgttcgccc cagacaacta actggtcgat tgctccagcg cggaaggtat
     8221 ctgctgctgc catcaatact tttttacctt cgtttttgaa ctgatgggca agtttaccaa
     8281 tacttgttgt tttccctaca ccattaacac cgacaaacaa aataactgtt agtccgtctt
     8341 cttggatatt tagttcattt acttcattga ttccttccgc ttcatagaga tccacaagtt
     8401 tctcgatgat cgcattttgg acttctgccg attttttcgc atttctgagt ttcacttctt
     8461 gacgtaatgc ttcggtaatt cgtatcgctg tttcaaaacc tacatctgca ccgataagtg
     8521 tttcttctaa ttcttcaaaa aagtcttcat cgactgttcg aaaattggca aacaactcat
     8581 tcatccgttc accaaatgtt ttacgcgtct tttctaaacc cttgtcgtac ttctcctgta
     8641 cttcaacttc ttctgttttc tcttcgccag taaaagcacg cttgatttta tcaaaaaatc
     8701 ccatttgact ccccctactc taatacaaat ttttggatca ctttggctac gccattttct
     8761 tcattgctgg ctgtcacgaa atctgctgct tctttgacga aaggcacagc attctccatc
     8821 gctacgccta gtccagcata ttggatcatc ggtaaatcat tttcctcatc accgatcgcc
     8881 atgatttctt cttgtttcaa accaagatct tttgccaata aagaaatacc ataagctttt
     8941 gtaacacctt ttggcataaa ttccaaaagc atacttcttg ttttgatgat ctcatacatt
     9001 tcatggaatt ctgctggtat ttctttgatt ttaggatcta attcttcttg gtcataagcg
     9061 attactgcct tattaagcac aaattcatct gtaatatcgc ttatatccgc tggttggaat
     9121 tgcagcagct tattcaacac attgtacaag gattgcttcc ttggggcact aggcagctgt
     9181 aaaaccacat tgtcagacaa aacatctagt ggcagatcca attgctgagc cagtttgtat
     9241 aagcgttgga tatctgatac ttccatcact ttcttttcca tgatttcacc agtatcattt
     9301 ttttgtacca aaccgccgtt gaaagtgatg ctgtagtcac cttcatctac cagtcctaat
     9361 tcttgcagat aatgcgccat cgcagctaag gggcgtcctg tacatagaac gatcttgacc
     9421 ccttgctgtt ttgcttgtgc taatgcttgt ttattctcat ctgaaatcag cttttgttca
     9481 tttaataatg taccgtccaa atcgattgct actaatttga tcattttcgt tcctacactt
     9541 tcgtttcgtt gattttcgcc tcttcattga tatcttccat ccgtacagaa acgatttttg
     9601 atacaccgga ttcctgcatc gtcactccgt acagtacatc tgctgcgacc atcgttcctt
     9661 tgcggtgtgt gactacgata aattgtgtgt catcttggaa atcgctcaag taacgaccaa
     9721 aacgtttcac gttggcttca tctaacgctg cttctacctc atctaagata caaaacggaa
     9781 caggacagac acggataatg gaaaagagca acgcgatggc tgtaagtgct cgttctcctc
     9841 cagaaagcaa acttagactt tgcagttttt tgccaggagg ttgtacttca atctcaatac
     9901 ctgttttcag caaatcagtt ggttccgtca gtactaattc agctttaccg ccgccaaaca
     9961 tatttggaaa gacaattttg aattcttgac ggatagcttc aaatacttct ttgaatcttg
    10021 tccgcacttc atcatccatc tcatccatcg tttcaaacaa ttggtttttc gctgctaaca
    10081 gatcatctcg ctgtgttgct aagaatgtat gtctttcact gacttgttcg tattgttcga
    10141 ttgaatttaa gtttactggt cccagttttt caatctgttc ttttaaggat gaaacctttg
    10201 ttctactgtc ttcgatatcg gttgtttctt gatagtcttg ggaagctttc tcaaatgtca
    10261 gctgatattc tgactgcaga taggtcaaat gattatctaa aatcatttca gctcggtctt
    10321 tttggacttc taatttggat tgttccgaca gtcgtgcttt ttgctcacga ttacgctctg
    10381 ccaagactgc ttcaagctta tcgatctcct tttgccttct atctcgctgc tctttggctt
    10441 ttgccaactc ggcttttaat gtttctcgct gagcagataa ttcgttaatt tgtttttcca
    10501 aactttcttc tgtgacttca tgatcagaaa agtctgctgt caatgtcgcc aactgctttt
    10561 ctatcgcttc ttgacgttcg gttgcctctg ctttttggac tcgggcaccg cggagctgga
    10621 tctgcagatg attaaactgc tctttcaaga cagcaagatc tgcttgttct tgtgcttttt
    10681 gactttgtac ttgtgctctt ctggcttcca tctggtcgct ttcttgactt aacgatttga
    10741 tttcttcgtc gattttttgt cgttgcgatt caagatctgt ttgtttgtct ttcaattcgt
    10801 tcgtttgctt ttcgtaatct tcgataaact gctgcagttc acgtgtctca aaagttgaga
    10861 tttgcttttc tttttcaaaa cgttcaagtt catttatgat gttttgtagc tgatttgtcg
    10921 cttcttgttc ttcaaaacgt aattgttcgc ctcgtgtacg caaaacttct tgttcttcgg
    10981 ctaaacgagc tgtttcttgt tgcagctcct gaactttttt ttcttgtgct tggagttgtt
    11041 tatctgcttc ttcaaattca ctagtcaact gtttcaattc ttgattttgt acaaacaaac
    11101 tgccttggtt tcctcgttta ttcgctccac ctgtcatgga tcctccggca ttcatcacat
    11161 caccttctaa ggaaaccact cgatattgat agcggatcgt ttgcgcgatt gcattggcac
    11221 tagttaagtc tttggctaaa aggatcgttc ctagcaggtt ttgaaccaca gtttggattt
    11281 gatcgggata actgacttgt tcactggcaa tccctataaa cccttctaca gctgctgctt
    11341 gtgtcaatat atgagccggt aattgtcttg gtttgattgt cgtcagtgga aggaatgttg
    11401 ctcttcctcc tctttgctgt ttcaaatacg tgatggcttg ccgagcatct ttttcatttt
    11461 caacgatgac atgctgggct gccccaccta aagctgtttc gatagctaac gtgaaatctg
    11521 caggaacatc gatcagttca gccactgctc cgacaatacc cgaaagctgc tgtttatgct
    11581 gcaagaccaa tcgaacccct tggtaaaaac caaaatagtt ttcttggatt tcttgaaggc
    11641 ttttcttacg agcacgtagc tgctggactt gattcattaa ttgatacatt ttaggctgtt
    11701 cttttgctaa tgcttcttgg aactttttcc cgttttgttg gatcatttct aacttctctt
    11761 ttgtttcagt taaagatgat tttagctgcg cctgcttttg tgtcaattct tctttttgta
    11821 agatcaatcg gtctactgat gcttctactt ccgattgctt ggctaacgtc tgcttgcttt
    11881 ttgctgtttc ttgtatatat tgacgttcta gatacttcag ttcgtttcct acagctgctt
    11941 gttcttgcat caaatcaaca tattgatctc ttaattctgc caataattct ttcgttgatt
    12001 tttggtattt ttctaattct tgctgtgttt ttatgagatt tgcttcagct ttttggatct
    12061 ctgtttcttt ttctgctgct gctttcatca agctttcttg aagcttttca aaatgtttaa
    12121 cctttttttg tgcttctgct aatgaagttt gatattcttg cgagcttttt tgtgtatgct
    12181 ttgtccgctc ctgcaaaaca tctttttgtc cttctgtctg cttcagtttt tccgaaagat
    12241 ctaataatac ttgctgattt ttttcgatca gacgatcggc ttgcgcattt tctttacgct
    12301 gcttcgccaa aatggattct tgttcttgga tcgactcaga tagtttgcct aattctagat
    12361 tgaatttcgc aagctgtgct tgtttgttgt cccaatcttt ttttgctgtc ttgatttcag
    12421 caaccatcaa agagacatcg gtttgagtca gtgtttcttt tagccgtaaa aactcctttg
    12481 ctgcctcact ctgggcggcc aaaggagtta actgttcttc caattcgtgg atgatgtctt
    12541 ggacgcggct gaggttgtct tctgtttcaa aaagcttctg ttctgctttc tttttccgct
    12601 gcttgtattt caagacacca gctgcttctt caaagatccc acgacggtct tccggtttac
    12661 tactgaaaat cgcttctact tttccttgag aaatgattga gaacgattct tttcctaatc
    12721 cagaatctaa gaacaattct tggatatctt ttaatctgca gctttgttta ttgatgaaaa
    12781 actcgctttc cccagtccgt cgataacggc gggttacact gatctcattg tactccaatg
    12841 gcagataatg atcactattg tctaaaatca ctgtgacttc tgcaatattc agctgcttac
    12901 gggtatcgga accggcaaag atgatatccg gcatttttcc gcctcgcaga cttttagcag
    12961 attgttctcc taatacccag cggatagcct ctgtaatatt gctttttccg ctgccatttg
    13021 gtccaaccac tgctgtgacg ctattttcaa aatcaatgac tgtcttgtct gcaaatgatt
    13081 taaaaccggc tatttcaatt cgtttcaaat acaaagcttt ccctcactta cttttacagt
    13141 gctgccaatg cattttccgc cgcagcttgt tctgctaatt ttttcgattt cccttgtcca
    13201 gtgccaatca attgatcatc tacataaact tcgatccaaa atactcgttc atgagctgga
    13261 ccttcttcat tgatcaatcg gtattcgatt gatacatcac ctgatttttg caagacttct
    13321 tgtagttttg ttttatgatc catctcatgt gaaaaagcac ctgctttgac tttaggaaaa
    13381 atcacttttt cgatgaatgc atgggtcgtg tcgaatccct ggtcaagata taacgcgcct
    13441 aaaaaagctt cgaataggtc gcaaagcaat gctggtcgcg tacgtcctcc tgaattctct
    13501 tctcccttgc ccaacaaaac atattgatca aagtgacatt ctttggcaaa tttcgacaag
    13561 ctgtcttcgc gaacgatcgc agctcgtgtt tttgtcaatt ttccttctgg caaatgagga
    13621 tataaacgga ataaatactg agaaaccatc agttctaaaa cggcatctcc taaaaattca
    13681 agtcgctcat tatccgataa ttgcagattg cgatgctcat tcacatagga tgaatgggta
    13741 aaagcttgtt caagcaaatt tagatcgtga aaaacgatgt tatagttttc ttttagttct
    13801 tttgtcagct ggttgtccat tttccacatt ccttttatgg atattttttc gatacttcct
    13861 tattatactg gttttttgtc aaatttgaaa tggttcggtc aaaaggtaag taaagcttta
    13921 ataatcctag tgattgacga gaagttgtga aaaattcgtt tagtttcgag tatgaagaaa
    13981 aaattttgtg aaaatggctt tccatatttt tgcataaatt tgacttaata ccgagaaact
    14041 ggactttgaa caccgtttat tggagaagtc atgaaatcag cgccttgacc tgaatattag
    14101 agaaataaac agaaaaatat caaaaaaccg aaaaaggaca tcgccctttt tcggtcattt
    14161 ttagtgatta tgaacgtgaa tctgctgtaa cagaaacatt tgcccatggg tttggtacat
    14221 cataagacca atcccagtct ttgacacggt cgttgactgg caatacttca ttacggaaca
    14281 acgttggaat cgcaaatgct tcttcaaatg catattgttg ccatttttga tatgcttctt
    14341 tttggtactc ttcatcaaat gctttgcttg aactgatatc gttgatcaat tttgtatttt
    14401 cttctgattc ataacgagtg tagttgaacg cagcatttgg tccccatagt ccacttggat
    14461 ttggatcagt tcctgtattc caagcagctt ggtaaacatc aatttcagga tcatcgtttt
    14521 ctaatttatc atagaaagaa ttgaattcga tcagacgacc tgttgtgtat tggacatcca
    14581 aaccaatttc tttccattgt tggatatagt aatctgccaa tggttgcgct gtttccccac
    14641 cggacatcga agcaaagttg attgttagtt tatttccatc tttgtcttct cttaaaccat
    14701 cgtcatccac atctttgtag ccagcatcat ctaatagttt tttcgctttg tctagatctt
    14761 gtgtgaatcc tttcatctca tcatcatggt atgactggaa tactggagga atcaatgaag
    14821 tcgcattgct tctcaaacct gcatagaaac gttcacctac tgcattatta tctaatgcat
    14881 agcccattgc ttgacgtaat gatttatcag ccatttttga atctggattg tattccacac
    14941 tctttgtatc tgcattccat ttacctaact tgaatcctag ataagtataa gaagtttgtt
    15001 cacggcctaa gtttgtatag ccagcaattt ctttataggt atcgtaagta tctgttggca
    15061 tttggaaaat catatcatat aatttagagt tcaatgcttc agttgcactt gccatcggta
    15121 cagatttcat cgtgatcttt ttcaatttag gcgtaccatt ccagtagtgc tcgtttggca
    15181 agtattctac tgattctcct gcaacgatat tactcatgta ataagggcca aaagtcactg
    15241 gatttttacg tacttgatcg cttgattcca tatctttgat tgctacacct tctaacgtat
    15301 gttttggtgc tgcatatgac cagataccac cacccgattg aagcatgctt gggtttactt
    15361 ctttatattc gatttcaact gttttgtcgt ctattttttt gattccggag attgtgtcgg
    15421 cacttccatc atgatattct tccataccta cgatgttacg gaaagtgtcg tcataacgga
    15481 taccagtgta atctttattc ccaatgattt cgtaagggaa aatcacatcg tccgccgtta
    15541 catcttcacc atctgaccat ttgacgttgt cttttacttt gattgtcgct tttttcgtat
    15601 cttcatctag atcaagcgtt gcagctccgt cgtctgtgat tctgaaatcg tcatcgtttg
    15661 caaatagcgg ttcatgagaa ggagccataa aagctgcatc atatgaatct tggtaaaact
    15721 cccactggaa caatccttta aactgactgt ccgttgcgac cgcagcttct aattcgccgt
    15781 cttcgattgc atcatcatta ttttttgttg caattggaaa agttaattcc tctttgttat
    15841 tgccgttgcc tccagagttg tttccagagg tatctgtacc tccgcccccg catgctgcaa
    15901 gaacgacagc agacatcagc gctgcaaatc caaaaatcgt tttcttcatt cctatttccc
    15961 ccttcgcttc tatactagcc taaccgttgg cgcgcatctg cagaacgctt gaatgcttgt
    16021 ccaacataat tgatactcaa catcaaaact aaaataagga ttgatgcggg taaccagatc
    16081 cattgccgat tcactaagac ttctccgtta cttgcataac cgatcaatgt acctaaacta
    16141 ggtacatttg ccggcaagcc gaaacctaag aaagtcaaag ttgtctcgat accgatgttt
    16201 gccgcaaagt tcatcgtcaa attagtaatg attaatgaac ttagattcgg catgatttcg
    16261 cggaacatga ttttaaaatc attggttccc attgtttttg aggcactgac ataatctctt
    16321 cttgcttcag ataatgtctt gcttcggaat aatcgggctt tagctaccca ataaaatgca
    16381 ctcatgatga atataaagga ccagacattg tatctggaaa taatcgatac gaagacgata
    16441 atgatcagca tgatcggtag aatcatgata aaatcaacga tccgcatcaa gatgttgtca
    16501 atgatccctc cgtagtagcc agagatgatc cctaatccaa ccccgatgat cgaagtaagg
    16561 atcgtgatcg caaatccgat cgtaacagag tttctagcac ctatgatcaa ttgtcctaat
    16621 acatcacgtc cgccttcatc tgctcccaga atgaactttt gtccttgtgt cgaaactgtt
    16681 cctggtgcag cgtatttgtc gaagatacta acatacatca ctttgtcttg atcggtcagc
    16741 atcgcaccga taaagatcac taataataaa accactagca aacctaatga aaaaatagca
    16801 attttatctt ttttaaattc tcgagcaatc attcgtatcc ccataggagg aatactttct
    16861 tgaactgctg tattgttttt ttcactcatc gatccattct cctttctgat tggttttatt
    16921 gaatccgaat acgcgggtca acgatactca tgatgatatc tgaaagtaat gtccccacta
    16981 gcgtcgcgat tcctaaaatc aatactaagg atgtgatcac ggtatagtcc cgttggccga
    17041 tggaatcaat aaatagtttt ccgactcccg gataaccaaa aatcttttcg ataactacag
    17101 aaccagcaat caacgaagtg atttcatacc ccatttgaga agccatcggc aacgctgcat
    17161 ttcggaaaat atgtcgactg aatactttat tcgtcggtac ccctttggaa cgggctgttc
    17221 tgacaaaatc gagcgatttc gaatcgatta cttcacttcg cagatattgg attgtgacgg
    17281 cagtcgccaa aaatgcttgg gtaattgatg gtaacagaat atatttcaaa cggtctatcc
    17341 attgagtgat aaatccacct tgcaaaccta gtgtttgtga tccacttgtt gggaaccagc
    17401 ctaaacgata accaaaaaca aatagcatga tcaagccgaa gataaataaa ggaacagcaa
    17461 aactaaagaa gttgtagatt acgacaatct tatccaagat tgaattttga taccggccag
    17521 caagcatccc cagaggcaca gcaatcaaat aagtaaggac aactgtcaag atcgatagat
    17581 ataaagtgtt gatgatccgt tcgccgatca agtcagcaac agagcgtttg tataagaaac
    17641 tttgaccaaa atcgccttgc aaagcatttc cgatccaacg gaagtactgg acataccatg
    17701 ggtcattcaa gccagctgct tgtcgcatct gttcgatcac tgccggatcc tgatttggat
    17761 tgatcaaacc ggtaaatgga tcccccggca tcgcttgggc taataagaag atcagaacac
    17821 ttaaaataat aacttgcggg atcatcaata aaacacgtcg cagaatcgtt ttccacatta
    17881 gcttgcacct ccatctttca acgccacttt gtgtgtagtt gtgatcgtac gcaaatcgta
    17941 gactcgacca gtcgcatcat agtattcttt ttggttttgg atatactctt gttctactct
    18001 tcgtcgattt tctttgtggc tttccctatt ttctacatcg atttgcggaa tagctgaaag
    18061 cagacgtttc gtatagatat gtcgcgggtc attataaatg tcttctcttg taccgatttc
    18121 tacaaatcga cctttgtaca taattgcgat attgtcgcac atgtgtttta ctacacctaa
    18181 atcatgtgaa atgaataaat agctgaggcc gtattcttgc tggattcgtt tcatgaaatt
    18241 caatacttgc gcttgaacag atagatccag agcagataca ggttcatcgg ctacgatcag
    18301 ttttggattg gtagcaacag cacgggctac tcctagacgt tgacgttgtc cgcctgaaaa
    18361 ttcatgagga tatttatata gcgcatcttc cggcatccca acgatatcta gcagtccttt
    18421 tacctttttc ttttcttctt gatcgcttaa ccgttcaaaa tttcggatgg gttcagcaat
    18481 gatatccaac actcgctttt taggattcaa actggacatt gaatcttgga agatcatctg
    18541 cacatccttg ttataaccca tttttttgcg gttgcttcgc ttcgttacat cttttccttc
    18601 atacatgatt tgtccggctg ttaccttttc taatcccaca acggcttttc ccgtagtaga
    18661 ttttccagag ccagactctc ccactaaacc atatgtcttt cctttttcga taataaaatc
    18721 cacaccatct acagcatata catgatccgt tactcgattg aagaatccac tgcgaatagg
    18781 gtaatgaact ttcaggtctt tgattgtgat taattctgtc atctacacct ctcctttttc
    18841 gtctctaaag tggaaatgct tatagcagga gcaacgtacc aagtggttcg gtgcgacttc
    18901 atgaaggata ggctcttctt catgatcgct tgctggtatc catggaatac gtggtgcaaa
    18961 ccgacaacct ttacgaggca tgtttttcag agaaggaacg acaccttcga tcacatggag
    19021 ttctgcatca tgatttcctt cttgtggtat agagttcagt aaagagcgtg tataaggatg
    19081 cttaggatga tcaaacagtt cttttacacc tgctacttca acgaactgtc cgccatacat
    19141 cactgccact cgatcagcca tttctgctac gactcctaag tcatgagtga tcaagatgat
    19201 ccctgttttt gtttcttcct gaaggtcttt cagtaaatca agaatctgtg cttggatggt
    19261 tacatctagg gctgtcgtcg gttcatctgc aatgacgatt ggaggtttgc aggcgattgc
    19321 gattgcgatg atgacacgtt gtctcatacc gcctgatagt tcatggggat attggcgtcc
    19381 aactctcgca ggattaggaa tccctacttg ttctagcaat tctagtgcac gagcttgacg
    19441 ctgttctgca ttcatctttg tgtgatagga aagtccttct ttgatctggt cttcgattct
    19501 catgagagga tttaatgcag acaacggatc ttggaaaatc attccgatat catttccgcg
    19561 gattttatta tagagcgttt cgttcaggtc gattaaattc agatcgttat ataaaatctc
    19621 acctgtaatt tttgtgttat taggatcaag taatcccata atcgtagtag ccaaggtact
    19681 tttcccgcac ccagattccc caacgattgc taaaatctcg tttttgtcta aagtaaatga
    19741 aacgtcatct actgcatcgt aatagtcatc tttgagtcgg aatcctgtat gtaggttctg
    19801 aacttctaat aattggttat cggacaatcg aatacccctc tctgcttcct aaatggaagt
    19861 ttttcttaat aaaaaaggac attacacaga ataaatcgtt ttataacaaa ggttattctt
    19921 atctttgtgt aatgaattat taataattat atagaaaacc gctttacttt gcaacataat
    19981 ttctgtttat cgccagaatt atcagaaaaa tgacttttac gtgtcaaaaa gattaaaaaa
    20041 agaaggataa aaagctgcat gcagcttttt atccttcttt gcttttattt gaaattattt
    20101 aagatggctg ctgatataat ctactgcgcc accgactgtt tcaatttgtt cagcgtcttc
    20161 atctgaaatt tccgtaccga attcatcttc aagctctaaa acgaattcca taatactaat
    20221 tgaatcagct tttaaatcat ctttgatgtt caattcatcc gtcactttat cggtatctat
    20281 ctcaaaatga tttgaaataa ttttcgctac tttgttaaat acttcttcgc gagtcaactt
    20341 tttcacctcc aagaatcata cacttgtcaa ttacctacac catggctaat tttactggca
    20401 tttttcagga atgtccacct ttttttcaat ggaacggatc gcttcagata tccaaatact
    20461 attcttcgct tttttcaaat tgttcgacta attgtccaac tacatctgtc tcaagcattg
    20521 tgtggatttg gcggatcgta tagcgaacag catcggggcc tgttgctcca tgagttttaa
    20581 tgactggtgc tttcaagcca aacaaaacgg caccgccatg tttggaataa tccatttctg
    20641 ctttcaaaga acgtaagccg tcttttaaaa gcatagcgcc catttttcct ttgatccctt
    20701 catttagaat agcagatttt aatagattca tcatattcat tgctgttcct tcaatagatt
    20761 tcaaaacagc gttgcctgta aatccatctg tgaccactac atcagccaca ccattgagta
    20821 gctcacgagc ttctacgttt ccaataaaat tcaagcttgt ttcattctga aggagttcat
    20881 aggctttttt cgtcacttca ctaccttttg ttgcttctgt tccgttgttt aagagtgcga
    20941 cacgaggacg tccgatacct cggactttac gtgcataaaa agaacctaaa ataccgtatt
    21001 gcaacaaatg ttctggtttg ttttctgcgt tagcgccaag atccagcata tcgaaaccgg
    21061 caccttcttt ccctactact ggcaaggtag acatcaagcc agggcgttcg atatttttga
    21121 tacgtcctac gatgaacaac cctgctgcta gtagcgcgcc tgtattccct gctgaaaata
    21181 cagcatctgc ttcaccgttt ttcactgctt gtgcagcaag gaccattgat gcagtctttt
    21241 ttcgtcgaat tgcttttact ggctcatcat cactattgat tttttcgtct gtatggataa
    21301 tcgtgatgtt tttttcattt gttaagtatt tcttgatttc tgcttctttt ccgtaaagta
    21361 agaattctac ttctggaaaa tcttgctgcg ctaaagccac gccttctaca attgcttgag
    21421 gtgcgtgatc tccgcccatt gcatctacgg caattttcat tgtttccact cctttttccg
    21481 tcatcagtat aaagtatagc acagtctgct aatcgaaata acgattatcc tgattttcta
    21541 tttggaggct ttgcgctaaa ggctgatact caggaagcat ctgccaatcc tttatttgcc
    21601 aaagggctgc agcctcttct ctggctacct ccaaaacatt tccatccgtc accaagtcag
    21661 caatcgcaaa ttgaggcaga ccagattgac ggaagccaaa tacttcacca ggaccacgta
    21721 attccagatc tttttcgctt agtacaaaac cattggtggt ctctgtcatg attttcatcc
    21781 gttccacacc tagttcattt ttaggattag caactaagat acagtaggaa gcatcacttc
    21841 cgcgtccaac acgtccacga agttggtgca gctgtgccaa tccaaatcga tccgcatcca
    21901 tgatcagcat gacggtcgcg ttcggcacgt ttacccctac ttcaattaca gtagtcgaaa
    21961 ctaatatctg catctggttg tctttgaagg cttccatgat tacttctttt tcctgattct
    22021 tcattttacc atgtaacaag ccaacttcat attttggtgc aaatagttca cgaagttttt
    22081 catagatttc cactgcattt tttacatcta atgcttctga ttcttcaatc agcggacaga
    22141 tgacatacat ttgatgtcct cttgccagtt cttttttcgt ccaatccagt acactgtcca
    22201 attgaggtgt tcttacccag cgtgtttcga tcgggatacg tccagctgga agttcatcaa
    22261 tgatagatac atccatctca ccatatgctg taatagctaa tgttctagga atcggagtcg
    22321 ctgtcataaa taagacatct ggtcgccagc ctttttcacg aagtactttc cgctggttca
    22381 cgccaaaacg gtgctgctca tccgtaataa ctagacctaa attctgaaat tcgactccat
    22441 cttggatcaa tgcatgtgtg ccgatcacga cgttaatttc cccttgctct agtttttcaa
    22501 ggagctcacg tcgttctttt gctttcgttg accccgtaat caatgcgatg gttacttcat
    22561 tgggatcaaa cagctgttgc aagctttcca tatgttgttg tgccaagatt tctgtaggca
    22621 ccatcaacgc accttggaaa cctgctgtaa cggtagcgta taacgcaatc gcggcaacca
    22681 ctgtttttcc gctacctaca tctccctgca aaagtcgctg catatgctgc ggactgcgaa
    22741 gatcccgaca gatttcattc gttacacgtt tttgtgcatt tgtcagttca aaaggtagct
    22801 tttgagtaaa ttgtttcaat ttttggacat cataaaggat ctccaaacca ttcgtttctg
    22861 ctttttctgc ttttttcaat ccttgaagac gcatttgaaa taaaaagaac tcttcaaaaa
    22921 cgacacgtcg tttggcttga tggttttctt cgtggtcttt tggaaaatgc atacttctta
    22981 cagcagaagc tcggtcaagc agtcgatatt tctccacaag gttgtttggc agattttctt
    23041 caatcaatgt gccgtattct tgaaaccctt ttttgattag atcaactaat gtcgtttgac
    23101 gaattgattt gttgacgtga tatatcggcg aaaagtcatc cattgtttga gaacctaaaa
    23161 tcttcatccc gttcaaggct ttacgctttg catcccattt accgtatacg gcgatttcct
    23221 ccgataaaat gactttttct tttagataag gttgattaaa aaaagaaacg ttgaacacat
    23281 catgttcttg catcatacga aattgcaatc ggcttttttt ataaccaaat cgattcatca
    23341 caggaggcga gactacgatt cctttcaaag taaccttttc ttgatcctgg atctcgttta
    23401 gatttctttc ctgaatatct tcgtagcgaa aaggataata agtcagtaag tcttcaatcg
    23461 tttggatgcc caagctggca agactagctg ctcttttttc tccgactcct gccagcatag
    23521 tgattggatc atctaaagaa cgcataaatt tttcctccct cctttaacaa aaaaaggact
    23581 tggtcagcga tcatacctga tcactaaccg aaggcctttt atgaatgaat actgtgttag
    23641 aaagatattc tgacacagaa attattcagc agaaaataga taaggataga caggctgatc
    23701 tccttggtgg atctcgattt ccaaatcagg atgtttttct gttagtttct cagcaaattc
    23761 atttgcttgt tcaactgttc cttcatcacc tgccaagatc gttacgatct ctgtgtcctc
    23821 attgatcatt tgttctagtg tacttaacgc tgttgctgtg atatctgggt tagagatcag
    23881 gatttttcca tcgatcatgc ccaaaaagtc tccttctttg atctctacac cgtcaattga
    23941 ggtatctctg atcgcgtgag tgacttgacc gctcacaacg ctagacaaca tttctgtcat
    24001 cgtttctttg ttttcttcta aagaagcttg atcattgaat gaaagcatag ctgtcaatcc
    24061 ttgggaaatc gttcttgaag gaacaactgc tactgggata tctgctactt ctgctgcttg
    24121 atcagctgcc atgaagatgt tcttattgtt tggcaagaca atcactttat ctgcgtttac
    24181 ttcttgaatc gctttaacaa tgtcttccgt acttggattc attgtttgtc caccactgat
    24241 aacataatta gcgcctaaac tttcaaacag ctcttggacg cctttaccag cagcgatcgc
    24301 tacgatacca tacgggatac gtggtatttc ttttttcgca aactcgttca cttcttcatc
    24361 gtgttccaaa atcgtctcat gctgcaagcg catgttgtcg actttgattt tgactaatga
    24421 accgaatttt tgtccataat tcaagacttc tcctgggttt tctgtatgca cgtggacttt
    24481 gatgatctcg tcatcattga ccacaagtaa agaatctcct ataccatcta aatagttacg
    24541 gaattcttcg taatcgaatg tactatcgac tgtaggtcct tctccgattt ttaccatgat
    24601 ttctgtacag taaccaaaat ggatatcttc tgtcgccatt tgaccttgga cactgcgatg
    24661 atgttctgca ttgaccatat catccatttc tgctggagaa ggttcatatg tttcatccgc
    24721 ttggaattct ccattcaatg cacttaaaaa gccctcataa acaaacagca gtccttgccc
    24781 tccactgtct acgacaccga cttcttttag gactggaagc aagtctggag ttttagctaa
    24841 agcacgcttt gctcctttta caacagctgt cattacttca acgacatcat cagtactttt
    24901 tgctttcttt tctccagctt tcgctgcttc tctagctact gtcaaaatcg ttccttctac
    24961 tggtttcatt actgctttat acgcagtttc aactccatga gtgaaggctg cagcaagatc
    25021 agaagcattt aatgagacaa catctggaat ttgcttagaa aatccacgaa acaattgaga
    25081 caaaataacg ccggaatttc cgcgcgcacc catcagcaat ccttttgaaa gcattgctgc
    25141 taattctcct actttttctg aggttgaatc tactactgcc ttagctccac tagtcataga
    25201 tagattcata tttgttcccg tatcgccatc tggcacaggg aatacattta atgaattgac
    25261 gtattctgca tttacttgca gacgattcgc acctgcctgg accatttcct ggaactggct
    25321 tgcacttatt tctgttacac tcacctagat aatcctcctt cagatcgact gctgagttac
    25381 cagccagtct cctgcctagt ctggcagtac acgaacacct tgtacaaaga cattaactga
    25441 attggcagtt acgcctaata atgcctctag attgtatttc actttttctt ggacgttgcg
    25501 ggaaacttca gagattttcg taccatagct gataatcgta taaacatcta cagcaatacc
    25561 attatcttct tggcggacaa ctactcctct tgagtaattt tctttacgca aaatctcagt
    25621 aatattatct ttaatttgat ttttgctggc cattcctaca acaccgaaca catctgtcgc
    25681 ggcgccacca acaactgttg caattacatc attggtaatt tcaatggttc cagctgacgt
    25741 ttgaattttt acagccatga atgagcctcc ttaacgggta aaaaactccc atttttcttc
    25801 tatcatattt tatcatagcg caacttgaaa taaaaggcag ttacccaaag tactcaaaaa
    25861 ccttgcaaaa tttcaggtat aaccttgttt aatttacaat tattcttctc aattgtcaat
    25921 tgtatctatg aaaatgtact tgcaaattcc cattctatat gatatgttag tctggtatga
    25981 gctgaattat tctattgctc caaatcaagg caaaggagga aattaccatg gcaaaagttt
    26041 gttactttac aggtcgcaag acaaaaagtg gaaacaaccg ttcacacgct atgaactcaa
    26101 caaaacgtac tgttaaacct aacttacaaa aagttcgcgt tatggtcgac ggaaaaccta
    26161 aaaaagtttg ggtgtcaact cgtgctttga aatcaggaaa agttgaacgc gtttaattga
    26221 atgatacttg gaccgttcag atagtgaacg gttttttatt taataaaaat ttttaagggt
    26281 gggacttttg attcctaccc tttttttgcg tttatttttg ttatacgaac aaattatgaa
    26341 gttatttatt ctaaatttta tttttttgtg aaaac
//
LOCUS       DADOOS010000026        25360 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.026, whole genome shotgun sequence.
ACCESSION   DADOOS010000026 DADOOS010000000
VERSION     DADOOS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.026"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     regulatory      86..183
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            299..880
                     /locus_tag="J8C37_000913"
     CDS             299..880
                     /locus_tag="J8C37_000913"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAZ5304372.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            883..2190
                     /locus_tag="J8C37_000914"
     CDS             883..2190
                     /locus_tag="J8C37_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAZ5304373.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2483..2971
                     /gene="purE"
                     /locus_tag="J8C37_000915"
     CDS             2483..2971
                     /gene="purE"
                     /locus_tag="J8C37_000915"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAZ5304374.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2955..4088
                     /gene="purK"
                     /locus_tag="J8C37_000916"
     CDS             2955..4088
                     /gene="purK"
                     /locus_tag="J8C37_000916"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAZ5304375.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4101..5396
                     /gene="purB"
                     /locus_tag="J8C37_000917"
     CDS             4101..5396
                     /gene="purB"
                     /locus_tag="J8C37_000917"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAZ5304376.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5576..5953)
                     /locus_tag="J8C37_000918"
     CDS             complement(5576..5953)
                     /locus_tag="J8C37_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304377.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6209..6937
                     /locus_tag="J8C37_000919"
     CDS             6209..6937
                     /locus_tag="J8C37_000919"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAZ5304378.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6937..7191
                     /gene="purS"
                     /locus_tag="J8C37_000920"
     CDS             6937..7191
                     /gene="purS"
                     /locus_tag="J8C37_000920"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAZ5304379.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7193..7864
                     /gene="purQ"
                     /locus_tag="J8C37_000921"
     CDS             7193..7864
                     /gene="purQ"
                     /locus_tag="J8C37_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAZ5304380.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7865..10087
                     /gene="purL"
                     /locus_tag="J8C37_000922"
     CDS             7865..10087
                     /gene="purL"
                     /locus_tag="J8C37_000922"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAZ5304381.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10072..11511
                     /locus_tag="J8C37_000923"
     CDS             10072..11511
                     /locus_tag="J8C37_000923"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAZ5304382.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11543..12586
                     /locus_tag="J8C37_000924"
     CDS             11543..12586
                     /locus_tag="J8C37_000924"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAZ5304383.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            12583..13161
                     /gene="purN"
                     /locus_tag="J8C37_000925"
     CDS             12583..13161
                     /gene="purN"
                     /locus_tag="J8C37_000925"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAZ5304384.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13158..14693
                     /gene="purH"
                     /locus_tag="J8C37_000926"
     CDS             13158..14693
                     /gene="purH"
                     /locus_tag="J8C37_000926"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAZ5304385.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMIDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14709..15983
                     /gene="purD"
                     /locus_tag="J8C37_000927"
     CDS             14709..15983
                     /gene="purD"
                     /locus_tag="J8C37_000927"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAZ5304386.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16105..17019
                     /locus_tag="J8C37_000928"
     CDS             16105..17019
                     /locus_tag="J8C37_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAZ5304387.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17152..18717)
                     /locus_tag="J8C37_000929"
     CDS             complement(17152..18717)
                     /locus_tag="J8C37_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAZ5304388.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18710..20161)
                     /locus_tag="J8C37_000930"
     CDS             complement(18710..20161)
                     /locus_tag="J8C37_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5304389.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20175..21191)
                     /locus_tag="J8C37_000931"
     CDS             complement(20175..21191)
                     /locus_tag="J8C37_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304390.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21411..22499
                     /locus_tag="J8C37_000932"
     CDS             21411..22499
                     /locus_tag="J8C37_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304391.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22553..24079
                     /locus_tag="J8C37_000933"
     CDS             22553..24079
                     /locus_tag="J8C37_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304392.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24081..25286
                     /locus_tag="J8C37_000934"
     CDS             24081..25286
                     /locus_tag="J8C37_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304393.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         8064 a         4323 c         5801 g         7172 t
ORIGIN      
        1 caatatttga aacaacattt tttattcaaa tatggcaaac tgccttcacg tgcaaaacag
       61 ttgcaagaaa atgaatttac actattatta gttttacata cctacttata tatcgtcata
      121 atatggatga cagtttctag ccagtaccgt aaatgctgga ctataagtaa aagattggct
      181 atttcaccct tcttttctta tgataaagtg gagggtgttt ttctttaaca gaaacatagt
      241 aggtttttgc acaaaagcgg ttatttcggt attaataaat gataaaggag caaataaagt
      301 gaaagaatta gttgaacgta ttcaaaaaga cggacgtata ttaggcgagg gcgtattaaa
      361 agtcgacagc ttcgtcactc accaagtaga ccctgtattg atggaacaaa tgggaaaacg
      421 gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt gtgaccattg aagcttctgg
      481 gatcgcacca gctttatttg ctgcaagaga attagatgtt ccaatgatct tcgctagaaa
      541 agcaaaaagt ttgacgatgg atgaagaatt attgacagct tctgtttatt cttttacgaa
      601 acaagtgaca agtacgattt ctatttctcg taaattttta tcttcagaag acaaagtatt
      661 gattatcgac gatttcttgg caaacggaca agcggcaaaa ggattgatcg aattatgcca
      721 gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc gaaaagtctt tccaagatgg
      781 acgtcagtta cttgaagaaa tgggattgag agtcgtttct cttgcgcgta ttgcttcctt
      841 gacgaatgga caagttgagt ttcttgagga ggacgcataa tcgtggaaaa agaaacacaa
      901 aatggaaaag ctgcggtttt agggctgcag catctgctag cgatgtatgc aggagcagta
      961 gcggtgcctc ttttgatcgg tacaggacta ggctttaatc aagaacagat gacttatttg
     1021 atttcaattg atatctttat gtgtggatta gcgacattat tgcaattgac agttaaccga
     1081 tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga tccaagcagt agctccactg
     1141 atcttgatcg gaaccaatga aggagtagga gctatctacg gatctattat cgcttctggg
     1201 atctttgttg tgttagtttc tggtgtcttt tctaaaatta aaaaactatt ccctcctata
     1261 gtaacaggaa cggttatcac cgttattggt ttgactttga ttcctgtagc aatcgagaaa
     1321 atgggtggag gtaatgcaac agcagctgat tttggtgaca aaacaaatct gctacttgca
     1381 tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg caaaagggtt cattcgttca
     1441 atcgcggtat tgattggttt agtcggagga agcatcttag ctgctttcat gggaatgatt
     1501 gatctgagcg cgattggaga tgcgccgttg ttccatgttc cacaaccctt ttattttggt
     1561 aaaccaactt tcgacgtatg gtcgatttta ctaatgatca tcatttccat ggtcagcatg
     1621 gtagaatcaa caggtgtata ttttgcactt ggtgacatta ctgggaaaaa tgttggagaa
     1681 gaagaattga aaaagggata tcgtgcagaa ggacttgccg ttatcttagg cggtattttc
     1741 aatacattcc cttatactgg cttttctcaa aatgtcggat tggttcaact ttcgggaatc
     1801 aaaacaagaa aacccatcta tttctctgca ttcttcttga ttgttttagg attattccct
     1861 aaaatcggtg cgttagcaca aatcatccct gagccggtac ttggtggagg aatgttagtc
     1921 atgttcggga tggtggcagt ccaaggaatc cgtatgctgt tggaagttga tttcagcaat
     1981 gataaaaatc tattgattgt tgctgtttct gttggatttg ggctgggttt caatgttacg
     2041 ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt tcactggaaa tggcatcgta
     2101 atgagtagca ttacggctat catattgaat ttgatcttta atggattgaa aaaagatgaa
     2161 gaaatattgg aagaagcctt caagaagtaa tagagcaaaa gaaaggttgt gaaatcagcg
     2221 ttttgacctg attgttagag aaacaaacgg aaaaatagtt cctcgtattt tgccgtttgt
     2281 ttgagctaat accgagggtc agctgattga acaccgttta tccataaaaa ggaatgtgac
     2341 ctaacttttg tcacattcct ttttttcatc tttaataaac acgaacaata aaatgttttt
     2401 ctttgttaac attcgaaatt tctttgacat caaaaaaagt gattgttaaa atatgaatgt
     2461 agaaaaactg ggaggaaaaa caatgacagc aaaaatctct gtgattatgg ggagtacttc
     2521 agattgggaa acaatgaaag aagcatgtca aatattggat gaattcggtg tgtcttatga
     2581 aaaaaaggtc gtttctgctc atcgaacccc agatttgatg tttgaatatg cacaaaacgc
     2641 tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc atctgccggg
     2701 tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat cacgaacatt
     2761 aaatggtttg gattcgcttc tttcgattgt ccaaatgcct ggaggagtac ctgtagctac
     2821 tacagcaatc ggaaaagcgg gagcaattaa tgctggacta cttgcgatac agatgctttc
     2881 catgtatgat ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac
     2941 tgttatagaa agtagtgatc aacttggtta aacctttgtt gcctggttca acgatcggga
     3001 tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa atgggcttta
     3061 aagtaggtgt gctggatcct gtgagcgatt gtccagccgc acaagttgca gattggcaca
     3121 ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct tgccagaaga acagatgtca
     3181 ttacctatga attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat
     3241 ttattcctca agggacagac ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt
     3301 ttttggaaac gaataacatc gtgattgctc cttatgcaac gatcgtcagt ccgacggata
     3361 tccaagatgc gattgacggg atcggctatc cttgtgtttt gaaaaccact cgaggcggct
     3421 atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga tctagctcct gccatggacc
     3481 ttttaagaga aggaacctgt gttttagaag catggatccc gtttgaaaaa gagatttcga
     3541 tcatggtggc aggaaatggt caaggagatt tcacgacttt tccggtcgtg gaaaacatcc
     3601 accacaacaa tattcttcat gaaacgattg ctccagccgc gattgatcag gatgtgatcg
     3661 aggaggctga gcgtattgcc cgtgtgattg cagaagcagt gagcctttca ggtgttttag
     3721 gggtagaaat gtttttaaca aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc
     3781 ctcacaattc tggacattat tctattgaag cttgttctat gagtcagttc gatgcccata
     3841 ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg tttgataagc aatgcagtaa
     3901 tggtcaatgt tttgggagaa caactgctgg aaagctatcg cttgattgag aataagccag
     3961 actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata
     4021 tcactatctt gacagaagac atccagcaaa cgctggaaga aatcaaacaa acgaatattt
     4081 gggattagag gagaaaaaac atgttagaac gttatacacg ccctgaaatg gggaatatct
     4141 ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga gattttagca gatgaagctt
     4201 gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat
     4261 ttgatatcga tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta
     4321 cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg ggtccattat ggtctaacaa
     4381 gtacggatgt cgtagatact gcttatggct atctcatcaa gcaagcaaat gatattttac
     4441 gggaagactt gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa cacaaatata
     4501 cagtgatgat gggtcggaca cacggtgtcc atgctgaacc aactactttt ggattgaaac
     4561 ttgccacatg gtattcagaa atgaaacgga acatcgaacg atttgaacat gcggcaaaag
     4621 gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc ccgccattta
     4681 ttgaagagta tgtatgtaaa caattgggga tccgtccaca agaaatctct actcaagttc
     4741 tccctcggga tctacatgct gaatatttct cagcgatggc gcttattgct acaagtattg
     4801 aacgttttgc aacagagata cgcggattgc aaaaatccga aacacgtgaa gtagaagaat
     4861 tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat ccgatcggtt
     4921 cagaaaatat ggctggtcta gccagagtgg tgcgcggaca catggtcact gcctatgaaa
     4981 atgtcacatt atggcatgaa agagatattt ctcattcttc agcagaacga atcatcttgc
     5041 ctgataccac gattttattg aattacatgt taaatcgttt tggcaatatc gttaaaaatc
     5101 taacggtctt tcctgaaaac atgaagcgga atatgtctgc aacatttggt ttgatttata
     5161 gccagcgcgt tatgctgaaa ttgatcgata agggaatgac aagagaagaa gcttatgatc
     5221 ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt gcagttccgc ccgctgttag
     5281 aagaagatcc aaccatcaca tcggtattat ctaaagaaga aatagacgat gcctttgatt
     5341 atcatcatca tttgaaaaac gttgacgtga tattcaaacg agtaggtttg gaataaagaa
     5401 gagtgagttg caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa agaagcagat
     5461 ccgttagcgg ttctgcttct tttagtttat tatattttct tatcaataat aggaacctga
     5521 ttttaaaagc ggctacctct ttaccgtgcc tctttacata caaaaaatag aggaattaag
     5581 cgtaaatggc ttggctatta taactaaaat gctggttatg gagatgttgc agcaagtttt
     5641 cgagaaaagc gtattccatc tcttgtccat tgaataagat ctggatacga taatgatttt
     5701 tgaaacgctt ctgttcatga atctgactga ttgaaagttc gcctttgtgt tctgtctgat
     5761 aaagaaaagt ccttttacgg cattcagcac agcgcttggt tgctcggcgg atttttcgga
     5821 atgtataaaa actagtatct ttattgcata aataaacgtc tttgatcgac agcaaatcat
     5881 tatatagaca ggaacgttct tcagctgtta attctgataa aaaaagctga ccttgtttta
     5941 ttgcatcttc catcggcaag tgcctcctcg aacccattat ccaaattttt agttaattct
     6001 aatataccat cattaagtat aaatgtaaac gcttgttgat ggtataagat tttcgtttct
     6061 gataaaacac gaacattaaa agaatctaat gccttaaatg tacatatttt tgttgattta
     6121 taaaaagatg attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat gtatttgctt
     6181 ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa gaacttttgt atgaaggaaa
     6241 agccaaaaaa atatatctta cttctgatga gaacttgatt tggatagaat atttagatca
     6301 agcaactgcg ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag aattgaataa
     6361 tcaaatcaca agtgcaattt tccgttatct gactcaaaga ggaataaaaa atcattttat
     6421 tgaacaattg tctaaaaccg aacaattagc aaaaaaagta caaattatcc ctctggaagt
     6481 ggttatccga aatattgcag caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa
     6541 agaattcatc aaaccaatca ttgaattcta ctataaggat gaccagttgg atgatccatt
     6601 tatcaatgaa gagcatatct atttcttaca acttgcaaaa ccggaagaaa tcagtgagct
     6661 gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt
     6721 ggttttagta gatttcaagc tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga
     6781 tgaagtaaca ccggatactt gccgattatg ggatgaacgt acgaatgaac atatggacaa
     6841 agacgtttac cgcagagatc taggtgattt gatcccagtc tatcaagaag tatataaccg
     6901 tctatcggaa ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa agtatatgta
     6961 acctataaag attcagtgtt agatcctcaa ggggaggcag taaaaaaagc agtccatcgt
     7021 atgggatatg aaacaatcga agatatccga ataggaaaat actttgagat acaacttgct
     7081 gatgcagaag aaccaatcga tacagtgatt gaaacgattt gtgacaagct gcttgcaaat
     7141 gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg aggaagtata acatgaaatt
     7201 tgcggtcatc gtttttccag gttcaaattg tgacgcagat atgttatggg cagttcgcga
     7261 gatcatgggc gctgatgccg aatttgtacg gcatgatgag tcctctttag aaggatttga
     7321 tggggtttta ctgccgggag gattctccta tggtgactat ttgagatgcg gtgcgattgc
     7381 tcggttttct acaatcatgg aagaagtgat tcgttttgct gaagaaggga aaccagtatt
     7441 cggtacatgc aatggattcc agatattgac agaagcagga cttttgccag gtgtactgag
     7501 acgaaatgag tctttgcatt ttatttgcaa aactgtacca ttgaaagtca accccaagac
     7561 ggcctttact tcagagtatg aagccgacga aataatctat cttccagttg ctcatgggga
     7621 aggaaattat ttctgtgacg aagaaacact tcaagaacta aaaaaaaata atcagattgc
     7681 ctttacttat aatgacaaca tcaacggcag cgtggaaaac attgcaggga ttacgaacaa
     7741 agcagggaat gtgttaggaa tgatgcctca tccagaacgt gcagtagaag acttgctagg
     7801 atcagaagat gggaaacggt tctttgcttc cattttaaaa aacttcggaa aggtgaccgt
     7861 atagatgaaa acattgaaag aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc
     7921 cgaatgggga ttgactgatg aagaatatcg aatgatcgaa gaagatatct taggaagaat
     7981 gcctaattat acggaaactg gactgttttc tgttatgtgg agcgaacatt gttcctataa
     8041 aaattctaaa ccggttctac gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc
     8101 tggagaaggc gcaggcatcg tcgacatcgg tgatggacta gcagttgtct tcaaagcaga
     8161 aagtcacaat catccttcag ccgtggagcc ttatgaagga gctgcgacag gagttggcgg
     8221 aatcatcaga gatattttca gcatgggggc tcgtccaatt gcgttgctag attcattgag
     8281 gttcggcgaa ttagataatc caagaacgaa atatttacta gaagaagtag ttgcaggtat
     8341 cggcggatac ggcaattgta tcggcatacc gactgtaggc ggcgaaattg catttgatcc
     8401 ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca
     8461 tatccagaaa gggcaagccg ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac
     8521 aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa gaatttaatc aagaagaaga
     8581 acagcaacgt tctgctgtac aagtaggcga tccttttatg gaaaagctgc tgcttgaagc
     8641 gtgtttggaa ttaatcttgc atcattcgga ttctctaata ggtatacagg atatgggagc
     8701 agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag gacttatatt
     8761 gacattagat gaagtcccac aaagagaaac tgggatgaca ccttatgaaa tgatgttatc
     8821 ggaatcacaa gaacggatgt tgatttgtgt taaatcagga gaggaagaaa aaatccaaga
     8881 actatttcag aagtatgatc tagatgcagt cactatcggt aaagtaacag acgacgggca
     8941 atatcggcta tatcatcaag gagaagaagt agcaaatctg ccagtcgatg cattagctga
     9001 agatgcacca gtttatcata aagaaatgaa agaaccagct cgaatcgtag aatttcagca
     9061 gttagctcct tatcagccag ttatcgaaga acctggacaa gtattgcttg atctgctgca
     9121 gcaaccgacg attgcttcaa aacgttcaat ctacgaaact tatgattcac aagtacaaac
     9181 caatacagtt gtccgtccag gaagcgatgc tgctgtgatg agggttagag ggacgaataa
     9241 agcgttagct atgaccacag actgcaacgc gcgctatctg tatttggatc caaaaatcgg
     9301 aggacaaata gcagtggcag aagctgctag aaatattatt gctagcggtg gtaagccctt
     9361 ggcgatcacc gactgcctga actatggttc tcctgataaa ccagaagttt tttgggaatt
     9421 atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc
     9481 aggaaatgtc tctttatata atgaaacgga tggtcaagca atctatccaa caccaatgat
     9541 tggtatggtc ggattgattg aggatcataa acatatcact actcaggaat tcaaaaaatc
     9601 aggggatcta atctatatat taggtaagac gtttgcagat tttgatggca gtgaattaca
     9661 aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa
     9721 gagaaaccaa gagttagtat tgacagccat ccaaaacgga ttgattgaaa gtgctcatga
     9781 ctgttcagaa ggtggcttgg caattgcact ggcagaatca gctttcaaac atcagctagg
     9841 gatatctgta cagtttgaat tgtcctctgc tcaattattt gcagaaactc aatcaagatt
     9901 tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg gagatgctgc
     9961 tgttttagca ggtaaggtga cggatgaagc gataatcgaa atttcagcaa ctgacggtca
    10021 gatcaaaata gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat
    10081 gaagtgaaga gtttaaatga agaatgcggg atcttcggtg tttggggtca tccagacgcc
    10141 gctagtgtgg catacttcgg gctccatagt cttcagcatc gaggacaaga aggtgcaggt
    10201 atcgtgtcca atgatcgagg aaagcttaag gggcatcgag atttggggct gctttctgag
    10261 gtattcaaag atcaaagaaa attgcagcag ttaacaggag aagctgcaat cggacatgta
    10321 cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc agccttttct attcaaattt
    10381 catgatcagt cgatcggctt agcgcataat ggtaacttga tcaatgctcg ttcccttaga
    10441 aaagagttag aggaaaatgg ggcgatcttc cattctaact ccgatacgga gatattgatg
    10501 catttgatcc gacgaagtga agaaacagat tttttaaata aattaaaaag tgctttgaat
    10561 gaagtcaaag gtggctttgc ctatttatta atgacagaaa cggcaatgat tgccgctttg
    10621 gacccaaatg gttttcgtcc attagcaatc ggacaaatga aaaacggagc atacgtgatt
    10681 gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt ttataagaga tgtccagcct
    10741 ggtgagatag tgatcatcaa tgacgaagga atccagattg attgctttac gaaggacacg
    10801 cagctttcta tttgttcgat ggaatatatc tattttgcca gaccagattc aaacatcgca
    10861 gggatcaatg tgcatactgc acggaaaaat atgggacgga atttagcgaa agaatcgcca
    10921 gtaaaagcag atatggtcgt tggcgtacct aattcttctt tgtcagccgc aagcggttat
    10981 gcggaagcta gtggaatccc ttatgaaatc ggattagtaa aaaatcaata cattgcccga
    11041 acatttatcc agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa attatctgct
    11101 gtacgaggag tagtggaagg taaaaaggtg attatggtcg atgattcaat tgttcggggt
    11161 acgacaagca gacggatcgt ccatcttttg aaagaagcag gggcaaaaga agtccatgtg
    11221 aggattgctt ctcctccttt gaaatatcca tgtttttacg ggattgatat tcaaacgcgt
    11281 aaagaactca ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat cggtgcagat
    11341 tctttaggtt ttttaagtga agagggtctg atcgaatcga tcggtctgga aaaagatgct
    11401 ccgtattcag gattgtgtat ggcttatttc aacggagatt atccgacgcc gttatatgac
    11461 tatgaagaaa aatatcagga atcactaaaa gaaaaagtat cgttctttta aaagcagacc
    11521 aagaagggag tatatgaatt caatggcaaa tgcatacgca aaagctggcg tagatgtcga
    11581 agctggatat gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg
    11641 tatgatgggc gctataggag gattcggcgg ttgttttgat ttatcggtct ttcaattaaa
    11701 agagccagtc cttgtctcgg ggaccgatgg cgtggggacg aaattagcag tggcaatcga
    11761 agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt
    11821 agcacaaggc gcagagccgt tatatttctt agactactta gcaacgggga aaaatgatcc
    11881 gttacgtttg gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag gaaatatggc
    11941 gctgattggc ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg attatgatct
    12001 agccggattt gctgtaggga tagctgaaaa atctcaatta ttgacaggag aaacgatcaa
    12061 cccaggagat gtgttgcttg gaatagcatc tagcgggatt cattcaaatg ggtactctct
    12121 tgttcgcaaa attttctttg agcagcagca gttttcttta tcatcaaaac tgccagaatt
    12181 gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa atctatgttt cttccttgct
    12241 tccgctgatc aaagaaaaga aaataaaagg agctgcacat atcactggtg gagggtttgt
    12301 agaaaatatc ccgcgaatgt taccagaatc tatggctgca caaatcaaac ttggctcatg
    12361 gccgattctt ccaatattcg gctgtttaca aaaatatggt gaaattcctg agatggagat
    12421 gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa
    12481 agaaatagta cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat
    12541 cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa cgatgagaat cgctgttttt
    12601 gcttctggaa acggaagcaa ctttcaagcg ctagccgatt atctatcgaa aaaaggattg
    12661 gaatcttcga ttgactggtt gttttgtgat cagcctgaag catatgtttt gaaacgtgca
    12721 actgcgttga gtgtaccggc agattgtttt tcacctaaag aatttgattc aaaaaaagag
    12781 tatgaagaag caattttgca taaattgaaa gaaaaaaaga tcgatttgat cgtattagct
    12841 ggatatatgc gaatcattgg accggtattg ctgaaaaatt atgataaaag aatcatcaat
    12901 atccatccgt ctcttttacc tgccttccct ggtttgcatg gcatacgtga tgcctttgaa
    12961 gctggagtaa aagaaacggg tgtcacaatt cattatatcg accaaggtgt agatactgga
    13021 ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg acacgttcga ttctttagaa
    13081 gaaaaaatcc atagagtaga gcatcgaatc tatccagaag tgattagtga aattattgaa
    13141 aatggaggct atctgaaatg acaagagcat tgatcagtgt atcagataaa actgggattg
    13201 tagcattcgc ccaaggtttg attgaacaag gagtagaaat catttcaaca ggaggaacaa
    13261 agcatgcgtt ggataaagct gggatcaaaa cgctttcgat cgaagaagtg acagggtttc
    13321 ctgaaatgat agatggccgt gtcaaaacac ttcatcctaa aatccatggt ggtcttttag
    13381 gaagacgcga tctccctagc catatggagg caatggaaac ggaaaatatc caaccaatcg
    13441 actttgtttg tgtcaattta tatcctttca aagaaacgat tatgaaacca gatgtcgaaa
    13501 tagcagaagc tattgaaaac attgatatcg gtggtcctag tatgctccgt agtgcagcaa
    13561 aaaaccatga atttgtcacc tctgttgttg atccatctga ctatgaagaa gtactttcag
    13621 aattacgtca gtatgggcat acttcacttt ctttcagaca aaaactagcg gcaaaagtct
    13681 ttcgtcatac tgcggcatat gatgcactga tcgctcaata cttaacggat tgggtggaag
    13741 aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa acaaacgctt cgttacggtg
    13801 aaaatagcca tcaagccgct gccttttatc agtccgctct tctggaaaaa tattcgatcg
    13861 cagcagcaaa acaactccat gggaaagaac tttcttataa taatatccga gatgcagatg
    13921 ctgcgctacg gatcatgaag gaatatgacg agccaacggt agtcgccttg aaacacatga
    13981 atccttgtgg tattgggaca gcatctacga ttttagaagc gtgggaagca gcgtatgaag
    14041 cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta gatctgccaa
    14101 cagcagaaca gatgcataag ctgtttctag aaatcatcat tgccccttct tatacacaag
    14161 aagctttgga attactgcaa aaaaagaaaa acatccgatt actgacagta gactttgcgc
    14221 aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt aggtggactt cttgtacaag
    14281 atcaagatct ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga
    14341 ctccagcaga agcaaaagca ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta
    14401 atgcgatcgt actggcaaac gaacgtcaga cagtaggaat cggagccggg cagatgaatc
    14461 gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt
    14521 tagctagcga tgcttatttt ccaatggatg attcggttga atatgcagcc aaacatggta
    14581 taaaagcaat cattcagcca ggaggaagca tcaaagacca agattctatt gatatggcga
    14641 ataaatacga tctagcaatg gtctttactg atgttcggca ttttagacat tagagaggag
    14701 tcggataaat gaacattttg gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa
    14761 aacttttagc cagtccgaag gtggatacgg tgtattgtgc gccaggaaat ccggggatga
    14821 caaaagatgg tatcaattgt gtcgctatca gtgagcgaga tcattcagcg ttaatccatt
    14881 ttgttaagaa agaggctatt gattggagta tcgttggtcc tgaagttcct ttgttaaatg
    14941 ggatcgtcga tgattttatg gcggaaggat tgaaaatctt cggtccgaca aaagcagcag
    15001 ctctgatcga aggttcgaaa agttttgcta aacaattaat ggatacttat caaatcccga
    15061 cagctaaatc cagaagtttt gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg
    15121 gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta gtcgtggcta
    15181 ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat cgatttgggg
    15241 atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg agaagaattc tcccttttgg
    15301 cgtttgtccg taataccaag gtgtatccga tggtcatttc gcaagatcat aagcgggcat
    15361 ttgataacga taaagggcca aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa
    15421 tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa
    15481 tgaaagaact aggacgctct ttcacgggtg ttttatatgc gggtttgatt gctacgaagg
    15541 aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc
    15601 tttctcggtt gaagagtgac tttgcgcaag taatcgatga tttattagag aatcgtacag
    15661 tagagttaaa gtggcagcaa gaaggataca atgtaggcgt tgtggttgca gctgccggtt
    15721 accccgaaga atatgagaca ggaatggttc taccagattt tctggaagaa gagctttctg
    15781 tttactatgc tggagtttcc tcaaataacg gcaaactagt tggagcagga ggacgtcttt
    15841 atttagtaga agcatacggt gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt
    15901 taatggataa aacgacagat ggaacatttt atcgtacaga tattggagaa aaagctgcca
    15961 atagcgaact gatcgtgaag taaatcagct gcttccattt ataaaaaata caacaaacat
    16021 aatcggaagc gccgatttta gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt
    16081 gtatataaat aaggaggagt tcagatgaaa aaaattcaat taggtacttc tgatcagaaa
    16141 gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg
    16201 attgagacag cttatgaaaa cggcattgat ttttttgacc atgcagatat ttatggaaac
    16261 ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa acgagaagat
    16321 ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa
    16381 catatcattg aatctgtaga aaaaagtttg acccgtctga aaatggatta tgtggatgct
    16441 ttacttcttc atcgtccaga tacattggta gaaccagagg aagtagctga agcgtttgat
    16501 gaattggaac ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa accaatgcaa
    16561 atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct gcaatttggc
    16621 ttgaaacata ctggaatgat cgatcaaggg attcatgtca atatgacaga tgaagcaagt
    16681 atcgatcatg acggtagtgt acttgattat tcccgattgc atgatatgac gatccaagca
    16741 tggtcaccat accagtatgg attttttgaa ggtgtattta tcgggaacga aaaattccct
    16801 gaattaaacc aaacattaga agaactagca gctaaatatc aaacgacacc aactggcttg
    16861 gcttctgctt ggattttacg ccatccagcc aatatgcaag tgatcgccgg tacgatgaac
    16921 gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta atatgtctcg ggaagattgg
    16981 tacgaattgt accgtgctgc tggaaacatt ttaccttaaa gattgtcata agcaggtcaa
    17041 aagggctata aattaaaaag acagaataac ttgaaagctg ttttcagctt cgagatattc
    17101 tgtctttttt tattgcaaat ggaaagagta tcattgtacg gatttcgttt attagttatt
    17161 tttgttttta aggaaaagct gtgtccgata ttcttttgga gaaatccctt gttttttctt
    17221 aaataaataa ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat
    17281 acgatagtct gattgctgca ataatttttt tgctaattcg attctttgtt ctgtcagata
    17341 atcgatgaat gtttggttaa acgattgaga aaaaatcgta ctgaaatgag agggacttaa
    17401 tgcaacttgt tgagcgaccc aagttaagga catatctgga tcggtaaaat tttctttgat
    17461 aaaatccaat gcatggttca aaattggctg atatttgttc cttgaaggct gtgagggcag
    17521 agcagttaag aaagaaatga acatataaac tgtcgaacgg taaagcgagt cgtcggcgct
    17581 gattgctaag cgttgactgg tggatgtgca atgaaccatt tcttgcggga ttttcttttc
    17641 tttcaggcag acatgctgga gtagcttagt caagacaaat aaacgatata atcgtgttcg
    17701 agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga
    17761 taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc
    17821 ttcttgaatc aaatcctcat agctgataat ttgttcttta agggaactgg cggtttcaag
    17881 tatcatctgc gtgatctcaa aagatgcagg taattcgctg attctttctg cgacaggacc
    17941 aatggcgaca gaaaaattat ttttttgtcc cttttctaat tcatggacca acgtttgggc
    18001 tacctgataa ctggtctcga tcacattttc ttcaagagaa tcaactatta aaaatttgat
    18061 gaatcgagag gaaacagcag agaagatcac ttgttgattc tccctaaata aaaacttcaa
    18121 ataatcactg agatgagcgt aatcttcaaa gtctgtactg acttggtttg ctgctaataa
    18181 tacggtcatt tttttccctg ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc
    18241 actgatcgta agtgagcctt tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa
    18301 aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt
    18361 ttctattgct tgtttcaatt cgctactttt aatcggttta agcagataat catctgcctg
    18421 tacctggatt gctcctttca tataatcgaa atcgtcgaat ccactaataa atatgagacg
    18481 agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat
    18541 ttggatatca gtcagaatga tgtcgggctg taagtcgata attgaagaca gtgccatctc
    18601 accatctcca gcttcgccga cataagtgat tggataataa gaaccaagga cagttatttg
    18661 ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt tatacatgtt
    18721 tgtttcctcc aattgttttg ggagatagag ggatcatcag tgtgacagtt gttccttctc
    18781 ggtatttgct gtcaatcgtg ataggttttt gatcatttcc gtaataaaga aggagacgct
    18841 tgtttacgtt gtaaagtccg tagccgatac tgaaatctga atcgatccct ttggctaact
    18901 ctttacgtat ttctgccagt ttttctgcag ggatgccaat cccgttgtct ataactttga
    18961 attggatcat ctcatcaatt ttgcatgcgg taatacgaat aaatcctttg tgacggacgt
    19021 acttcgttcc atggtataca gcattttcaa tcaatggctg caagatcatt tttaaaatag
    19081 aaacaggaaa aagtgtttct ggtatatcga tcttatagtc aagcatatgg ccgtatctta
    19141 tttttaagat gactagataa tcattgatat ggctaatttc tttttcaaga ggaatccagt
    19201 cttgtccttt gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc atagtcagag
    19261 cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc atccaaactg ttataaataa
    19321 aatggggagt aatctgagct tgcaatacac gaacttcact ttgtgctaaa tggtattgtt
    19381 tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc cattttattc atgctagcgg
    19441 taagtgtttg taattccagt gtttccggca gggttaaacg aaagccaagg ttccctgaag
    19501 aaagttgttc tgacatctcg atcagctgat tgatcggttc ttggaccttt ctctctaaaa
    19561 agcgacgatt ctttttcgca tagaaaaaaa tggtactaag aactccaagt tccactattg
    19621 ttaatagaat gagcgagcgg acaagtgtcg tatttttttt gcttgctaag tcgatctcca
    19681 cacgaacaaa atcttgtaag atatctgaca gcagttgagt gacagaatct acctgattga
    19741 tgatctcttc attggtatcc ataaaatctt ttgccaagat attattgatc agctcgtttt
    19801 gataatcttc cagtgtatcg atcgtacgta gcgatacatc taacgtacta atttctttaa
    19861 gtgtatttgt attatttttg atctctcgaa tatcattcct aagttcttct aggatatttt
    19921 cctcattgaa ctcatctacg gttacttggc cagaaatcaa gtcccacata ctttccaaaa
    19981 ctttttgatc aaccttcaaa gcaattgcat ctgctttttg gatatttgcg atcgtctgtt
    20041 gataaatcaa aagctgttgc gaatataaca gtatgctcag tagcaaaggg ataacagcaa
    20101 atagaagcat ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca
    20161 tgatacttct ccagtcaata atttctggtt gggatgttag ctatattctg ctgggaaaag
    20221 acagtctcgg aaataaaaat atctttaggt aatgtttttc ctgtttgaat atttccaaaa
    20281 taacgagtaa cggtattggc tacataccag ccgacatcag gtttacactc gactacacaa
    20341 ttgacgattc ctgagcgtag cttctcgatc atatcttctt gtccatcaat agttacaatg
    20401 atcaaatctt ctccaggttt gattgatgtt tcttttatcg cagctaaagc accgtgagtc
    20461 atttcatcac tatggctgta taatacatcg atttcggata aatcatttgt ttctaaatat
    20521 cgtttcatct ggtcttttcc tttcatccga ataaaatcac cgacaattcg gtgaacaata
    20581 gtcatcggag gtttatggtc gatggttcca tcttttttta gggttggttt acgattgatt
    20641 gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg agttctctga accagcaagt
    20701 tctaggattc tgatttcctg tttctttgaa tttgtgaagt gattgctgac aaataaaccc
    20761 gcccgatttc cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca
    20821 ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc ctgctcgctt tgcttctttt
    20881 aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag
    20941 gcaatgaacg tccgtatatc ttggatttgc cgttcctgat tcatgtagcc atttcggtag
    21001 agaacttggt aatcttcttt ttcaagcgca gtgataattg attcagtatg ctttttccgc
    21061 cagctactct ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt
    21121 gtattaggtg tacaccccat catttggtaa gcgattacaa caagggctaa aaaaaataaa
    21181 acacgtttca ctttaaatca ctccatctgg atttgttagt actatcttat cagataaaag
    21241 ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa
    21301 gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg
    21361 ctttctttat tagctatact cggtgtatca attgaaatgg gggataaaaa atgaaaaaca
    21421 aatttagaaa gattgttggg actatcgcta ttttgaccgc aagcattcta ttaagtgcgt
    21481 gtggcaattc aggatctgca gatgatagta cggggtatgt aggaatcgct atgccgacca
    21541 aatcagcaga aagatggatc gctgatggga acaacatggt ctcggaatta gaaaaactgg
    21601 gttataaaac agatcttcaa tatggagaag ataaagtaga aaatcaagta gcacaaatag
    21661 aaaatatgat tacaaaaggg gtagatacac ttgtgattgc ttctatcgat ggttcggctt
    21721 taactgatgt gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt
    21781 tactgatgaa ttcagaaaat gtcgattatt acgcgacttt tgataatttt ggagtaggtg
    21841 tatcgcaagc agcttatatt gaagagcact taggattgaa agaaggaaaa ggaccattca
    21901 cgattgaatt atttggtggc tcgcctgacg acaacaacgc actaatcaat tacaacggag
    21961 tgatgtctgt tttacaacca tatatggaca acggtcagtt ggtcgtgcca tctggacaga
    22021 cgagtttcag tcaaatcgct acgcttcgct gggatggttc tacggcgcaa gcaaggatgg
    22081 ataatctgtt aagcgcaaat tatactgacc aaacattgga tgctgttttg tcgccatatg
    22141 atccaataag tttaggaatc atttcgtcat taaaaggcgt ggggtatggg agtgaaagta
    22201 aacctttacc tgtgattact ggacaagatg cgacagttgc tggcgttaaa tcgatcattg
    22261 ccggggaaca aacacaaacg atctttaaag acacacgtat cttagcaaaa aatacgattg
    22321 aaatgatcaa agcaatcagt gatggagaag aggttccggt caatgatact gaaacttatg
    22381 ataatggggt gaaaactgtc cctacgtatt tagcaaatac agtatctgtc gataaagata
    22441 attatcaagc agagctgatc gacaccgatt attataaaga atccgatctg aaaaattaag
    22501 aggaaccaaa caaggaacta aagagatcta agtcagagag aggataagaa ttatggcgga
    22561 ttgtatttta aaaatggcag atatcaccaa aaaattttct ggaatccgag cgttagattg
    22621 cgtgaatctg atgattgaac gtggggaaat ccatgcattg tgcggagaaa acggagcggg
    22681 aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca tatggctcct atgacgggga
    22741 aatcttttat gatggaaagc tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg
    22801 catcgtcatc attcatcaag aattagcact aagtccttat ctttcaataa aagagaatat
    22861 ttttctcgga aatgaacaag cagaaagagg cgtgattgat tgggaactga cagaaaagaa
    22921 aacatatgaa ctgctgaata aagtcggttt gtctatcgac ccgaatcttc tagtatccca
    22981 gattggcgta gggcaacagc aactagttga aatagctaaa gcattatcaa aatccgtccg
    23041 tctgttgatt ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct
    23101 gagattgatc cgagagttga aagagcaagg tgtaacctcc atcattattt cacataagct
    23161 gaatgaaatc gtggatatcg cagatcgaat caccatttta cgggatggac agacgattga
    23221 gacactagaa aatgaaatga tcagcgaaga gcatattatt cgcgggatgg tgggcagaga
    23281 tttagttgat cgttatcctg aaagaaaacc ggatattgga gaagtttatt ttaaagtaga
    23341 agactggacg gtctatcacc ctcttgatca cgagcgaatc ataaacgacc acatcaactt
    23401 gcatttgaaa aaaggagaga ttgtaggaat agctggactg atgggagcgg gcagaactga
    23461 attcgctatg agtctatttg gacattctta tggaagcaag atatccggac gtatattaaa
    23521 ggaaggggca gagatatcag ttaagaccgt tccttcagcc attgcaaacg gactcgctta
    23581 tgtctcggag gaccgaaaat ctttaggact gaatctgctg atggatattc gggaaaacgt
    23641 gacgctgagc agtttggata aaataagtca aaaaggagta ttagataaag aaaaagaagt
    23701 aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca tccatcaaat
    23761 tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac
    23821 acaacccgat attctttttt tggatgagcc aacccgtggg atagatgttg gggcaaaata
    23881 tgagatctac acgattatcg aagaaatggc agcaaatggc aaagctgtct gtcttatctc
    23941 ttctgaactt cctgaaatat tagggatgtg tgatcgaatc tatacgatga acgaaggacg
    24001 tattacagga gaagtctcgc gagaagatgc agatcaggaa gtcttgatga aattaatgac
    24061 gaaagaagag gtgggttaag ttggaacagg caaaaaatct tacggataaa aaagagaaaa
    24121 aactaaattt gaaaagtaga gcagctgatc tttttggtcg ctacagcatg gtgatcattt
    24181 tagcgggact acttattgcg tttcaattga tgacggatgg tattttctgg cgtccattga
    24241 atataactaa ccttgttctg caaaatagcc atatattagt actagctgca gggatgttat
    24301 tagttgtgct cttaggtcat gtggatttgt cggtagggtc agtgatggcg tttgttggtg
    24361 caattgcggg catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca gttatcgcat
    24421 gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt ttgggtagct tatgcaggga
    24481 taccggcgtt tatcgtgact ttagcaggat tgctgatgtt tcgtggactg acacaagttg
    24541 ttctaggagg ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg tctacgggtt
    24601 atttaccaga cttaagcgaa ggaagcagcc ttcacttctt gacattgatt ttaggaatga
    24661 tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg aaaaatcatt
    24721 tatttgaagt ggaatctcca acagctttct tggtaaaggc ggtatttaca gcagtcgttg
    24781 tactaggaat aacttatatc tttgcctctt atcaagggta tccgattatt ttgatcattc
    24841 ttggtttgat cgttgcagca tatgggtttt tgacaaataa aacagtagca ggtcgacaga
    24901 tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa acaaagcgaa
    24961 tcacgttctg ggtattcgtc aacatgggag tcatggcagc tttagctgga ttagttttag
    25021 ctgctcgatt gaatgcggca acacctcaag caggtacatc gatggaactg gatgcaatgg
    25081 cggcagtata ttttggtggt gcttcgactt ccggcggcat tggaacaatc atgggtgcga
    25141 ttgtcggtgg cttggtcatg ggtgtattga acaatggcat gtcgatttta ggcgtcgggg
    25201 tagactggca gcaggcaatc aaaggattga tcttgctttt agccgttgtg ctagacttgt
    25261 ataataagaa aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa agactgtgac
    25321 aagacatttg tcacagtctt tttttcgcta aaagcgaaca
//
LOCUS       DADOOS010000027        24544 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.027, whole genome shotgun sequence.
ACCESSION   DADOOS010000027 DADOOS010000000
VERSION     DADOOS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.027"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            62..1762
                     /locus_tag="J8C37_000935"
     CDS             62..1762
                     /locus_tag="J8C37_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAZ5304394.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1732..1950
                     /locus_tag="J8C37_000936"
     CDS             1732..1950
                     /locus_tag="J8C37_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304395.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1970..2860
                     /locus_tag="J8C37_000937"
     CDS             1970..2860
                     /locus_tag="J8C37_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAZ5304396.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2878..3162
                     /locus_tag="J8C37_000938"
     CDS             2878..3162
                     /locus_tag="J8C37_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304397.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3649..4263
                     /gene="gmk"
                     /locus_tag="J8C37_000939"
     CDS             3649..4263
                     /gene="gmk"
                     /locus_tag="J8C37_000939"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAZ5304398.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4268..4573
                     /locus_tag="J8C37_000940"
     CDS             4268..4573
                     /locus_tag="J8C37_000940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAZ5304399.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4826..7234
                     /gene="priA"
                     /locus_tag="J8C37_000941"
     CDS             4826..7234
                     /gene="priA"
                     /locus_tag="J8C37_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAZ5304400.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7249..7740
                     /gene="def"
                     /locus_tag="J8C37_000942"
     CDS             7249..7740
                     /gene="def"
                     /locus_tag="J8C37_000942"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAZ5304401.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7733..8671
                     /locus_tag="J8C37_000943"
     CDS             7733..8671
                     /locus_tag="J8C37_000943"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAZ5304402.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8671..10029
                     /gene="rsmB"
                     /locus_tag="J8C37_000944"
     CDS             8671..10029
                     /gene="rsmB"
                     /locus_tag="J8C37_000944"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAZ5304403.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10042..10782
                     /locus_tag="J8C37_000945"
     CDS             10042..10782
                     /locus_tag="J8C37_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAZ5304404.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10779..12848
                     /gene="pknB"
                     /locus_tag="J8C37_000946"
     CDS             10779..12848
                     /gene="pknB"
                     /locus_tag="J8C37_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAZ5304405.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13013..13912
                     /gene="rsgA"
                     /locus_tag="J8C37_000947"
     CDS             13013..13912
                     /gene="rsgA"
                     /locus_tag="J8C37_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAZ5304406.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13925..14575
                     /locus_tag="J8C37_000948"
     CDS             13925..14575
                     /locus_tag="J8C37_000948"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAZ5304407.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14576..15214
                     /locus_tag="J8C37_000949"
     CDS             14576..15214
                     /locus_tag="J8C37_000949"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAZ5304408.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15369..16223
                     /gene="mreC"
                     /locus_tag="J8C37_000950"
     CDS             15369..16223
                     /gene="mreC"
                     /locus_tag="J8C37_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAZ5304409.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16227..16736
                     /gene="mreD"
                     /locus_tag="J8C37_000951"
     CDS             16227..16736
                     /gene="mreD"
                     /locus_tag="J8C37_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAZ5304410.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            16991..18493
                     /locus_tag="J8C37_000952"
     CDS             16991..18493
                     /locus_tag="J8C37_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304411.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSTTEE
                     STTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAE
                     AYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKA
                     GDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(18561..20360)
                     /locus_tag="J8C37_000953"
     CDS             complement(18561..20360)
                     /locus_tag="J8C37_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAZ5304412.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            20483..20989
                     /locus_tag="J8C37_000954"
     CDS             20483..20989
                     /locus_tag="J8C37_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAZ5304413.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21068..21790
                     /locus_tag="J8C37_000955"
     CDS             21068..21790
                     /locus_tag="J8C37_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ5304414.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(21868..23268)
                     /locus_tag="J8C37_000956"
     CDS             complement(21868..23268)
                     /locus_tag="J8C37_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAZ5304415.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(23438..24412)
                     /locus_tag="J8C37_000957"
     CDS             complement(23438..24412)
                     /locus_tag="J8C37_000957"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAZ5304416.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         7983 a         4527 c         5242 g         6792 t
ORIGIN      
        1 aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc
       61 gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca
      121 agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg
      181 ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac
      241 tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt
      301 tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa
      361 ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa
      421 cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt
      481 aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca
      541 tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat
      601 tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt
      661 gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag
      721 acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa
      781 gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata
      841 tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa
      901 aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta
      961 tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga
     1021 tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc
     1081 agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac
     1141 actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta
     1201 tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt
     1261 taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt
     1321 agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa
     1381 cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt
     1441 tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga
     1501 tataaaaagt atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg
     1561 gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt
     1621 tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc
     1681 agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa
     1741 gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg
     1801 cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat
     1861 tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa
     1921 caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat
     1981 gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa
     2041 aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt
     2101 tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt
     2161 tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat
     2221 tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca
     2281 agtgtcaatt agtcggatgg cggaacgctt cgtgctggac gaaaacttcg taacggtaga
     2341 agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag
     2401 cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt
     2461 gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat
     2521 ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa
     2581 agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca
     2641 cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca
     2701 atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac
     2761 gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat
     2821 cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg
     2881 aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc
     2941 ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac
     3001 gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt
     3061 cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag
     3121 atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag
     3181 aaatacaata aataaacaga attggatttc ttcgcttagc ttctttgctc taggaataca
     3241 tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc
     3301 aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa ctattttcac
     3361 aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat acacggcgtt
     3421 caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga agctgtttat
     3481 gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt ggtacagggt
     3541 agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc agaaaaaaag
     3601 tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat gtcagagcgt
     3661 ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt acgaaaagca
     3721 atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac ccgtaaaatg
     3781 cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga gtttgaatca
     3841 atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta ttatggaact
     3901 cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt ggagattgaa
     3961 gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat ctttttgact
     4021 ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga tgcagatgaa
     4081 gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc tctttacgac
     4141 tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa cattattgcc
     4201 agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt aaaggagatg
     4261 tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa attcaaaata
     4321 ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg cacagcctac
     4381 tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa tcgatgcagg
     4441 aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga tggaagaaga
     4501 ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta tccgtgaaga
     4561 gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca aacccagctg
     4621 tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc tgaacttttt
     4681 gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg gcagaagata
     4741 agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct tgataagaaa
     4801 agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg tggacgtacc
     4861 tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg aaacagcttt
     4921 gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg tccaaggatt
     4981 tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg cagttattcg
     5041 tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg attatatgaa
     5101 aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga gtgtgatgaa
     5161 agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc aggtattgtt
     5221 tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc tttctcaatt
     5281 gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata cacgaaataa
     5341 agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg aagaagaatg
     5401 ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt tatctgaact
     5461 gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg ctgtcttgaa
     5521 tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc gcgaccctta
     5581 taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac aaaaaaatgc
     5641 tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt tgctggaggg
     5701 aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg ttttgtcaga
     5761 aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa tggtcgagcg
     5821 attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt cccaaggtga
     5881 aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag taggcgcacg
     5941 atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg aagaacatga
     6001 atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag caatttggcg
     6061 cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt tagaatcaag
     6121 agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag ccaaagcagc
     6181 tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga atcatcggac
     6241 ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca aaaaagaaca
     6301 aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta gggactgtgg
     6361 atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg atacacgttc
     6421 aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc ctaattgtgg
     6481 cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg aactccgaga
     6541 actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa gaaaaggggc
     6601 acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat tagggacaca
     6661 aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt tgaatgctga
     6721 tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat tgcttaccca
     6781 agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc aaacattcaa
     6841 tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt tttatcagca
     6901 ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg tgaaaataac
     6961 cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg ccaatcagct
     7021 aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca tgattttgag
     7081 agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac cgaatcttcc
     7141 tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag gtctttttgt
     7201 ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat gcgctatcct
     7261 atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga tgtaatcacg
     7321 gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa tgacggaata
     7381 gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga agtagatgaa
     7441 ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga aagccttgac
     7501 gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc agatgaagtg
     7561 accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt tggctattta
     7621 gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat tgagaaaatg
     7681 atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct tgatgactaa
     7741 gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc tgatagaatc
     7801 aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc ggaaacgagt
     7861 aattacgccg acaccggtaa aaaaagcggc tctgaagcac ggtatccgtg tgctgcagcc
     7921 agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc ctgatgtgat
     7981 cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac caaaattagg
     8041 ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc ctgttcatta
     8101 tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga tcaaaaaaat
     8161 ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag atgatgtagg
     8221 gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa cattgcccaa
     8281 tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt tttcgccaaa
     8341 catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa ttgacaacca
     8401 agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca tacgctggaa
     8461 attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta cgatcatccg
     8521 gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag ccatcaatga
     8581 attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg gaagcggtca
     8641 gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa aatgaaaaaa
     8701 tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg cgcctactcg
     8761 aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga tgtgggttta
     8821 tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt ttacgttgca
     8881 ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agatgcttct ttatctttct
     8941 gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa cgaggcggtc
     9001 gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg tgtcttgagg
     9061 aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat cgaaagactt
     9121 tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac tggtggatca aatcggatac
     9181 gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag tgcccgagta
     9241 gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga agagatcaca
     9301 gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca tttagcttct
     9361 agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat gcttgtagcg
     9421 ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc acccggaggt
     9481 aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt ggtcgcttta
     9541 gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct aggtgtctct
     9601 tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt cgaagcagag
     9661 cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct tcggcgaaaa
     9721 ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa aatccaattg
     9781 gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac ttatagcact
     9841 tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga aagacaccct
     9901 gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat cgaagagaaa
     9961 atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg ttgtttgcgc
    10021 aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg aagacgacgt
    10081 aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa gcttgctgta
    10141 ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat ggcggtaacg
    10201 aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat cgcccaatgg
    10261 ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca agaacagccg
    10321 gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga ctggttcacg
    10381 attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat catgcaattg
    10441 acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag cgaagaaatg
    10501 gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc tggaacagtc
    10561 gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt atgttcagat
    10621 ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca agaaggccca
    10681 ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg agcggataat
    10741 atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg ggcaaaaaat
    10801 taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca aatgtatttt
    10861 tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga tttgactttc
    10921 aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca acagaactcg
    10981 ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg cagtatctgg
    11041 tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat tatcctgttc
    11101 cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct ttagctcata
    11161 accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat aatgaaggtg
    11221 ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg atcacgcaga
    11281 cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga ggaagtatgg
    11341 caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg ttgacgggta
    11401 gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt caagatgatc
    11461 ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac gtgattttac
    11521 gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg tctgatgatc
    11581 tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct catgtgatgg
    11641 ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct gattcattca
    11701 aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag gaggaagaag
    11761 ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta ttagctattc
    11821 ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga ggagaagttg
    11881 ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc gcaaaagcga
    11941 aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa gaaggtaatg
    12001 tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa gtcactctgt
    12061 atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa agttatgaag
    12121 atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa aaagaagagg
    12181 aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca gaagatacag
    12241 aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca gaaacagtga
    12301 ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt gttacacaag
    12361 gattcaatat caataatatt agtgctactt atgactatag cgacactgta ccatcaggtt
    12421 cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact tacatcaatt
    12481 ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat acaaaaagcg
    12541 aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag acttccgaat
    12601 attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca ggaacgacaa
    12661 ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca gcgtcttcta
    12721 gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt agcacaagca
    12781 actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc tcagaaagtt
    12841 caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt gttgcattct
    12901 cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg agaagtttgc
    12961 atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact
    13021 tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg caaacggaga
    13081 gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg
    13141 agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg aaatcttgcc
    13201 gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa tcattgtcac
    13261 aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag ttaccttgga
    13321 atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag acgacccaga
    13381 aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga ttgtacccaa
    13441 tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat cggtctttat
    13501 gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg aactgaattt
    13561 agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac gtcatgtgga
    13621 actgattcct atctatggcg ggttagtagc agatacacca ggatttagct cgattgactt
    13681 tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag aagcttctgt
    13741 ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag taaaacatag
    13801 agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat tcttacaaga
    13861 aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac
    13921 aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac aaagagacat
    13981 tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg atggccgatt
    14041 tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg ttactaaatt
    14101 accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag catttgcaaa
    14161 agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc atcgggcact
    14221 tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag gaacaccagt
    14281 ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc
    14341 tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg aactcgctga
    14401 tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa tcgttccaga
    14461 aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcaggat catatatcta
    14521 tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag actaaatgaa
    14581 aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc tgtccaaata
    14641 cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag gctacccagt
    14701 agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc
    14761 aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata cacagttagg
    14821 tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg gagctacggg
    14881 cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc gcttcaaacc
    14941 ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt atttgcctgg
    15001 agagcatata gtaagacaga taccagatat gaagtattta gcttattgct gcttgactcc
    15061 cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag aagtagaata
    15121 tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt ctttttcaga
    15181 tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa aaaatttgtc
    15241 tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag aggagcaact
    15301 taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa ttaattcgag
    15361 gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg gttattgtta
    15421 tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca
    15481 atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca atctctgccc
    15541 cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca acttatgccg
    15601 aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag caaaacaaga
    15661 attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag acgctgacta
    15721 gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg caggacatgc
    15781 tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa
    15841 aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc gaattactgt
    15901 catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct aatggcgaag
    15961 cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg acccaattaa
    16021 ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt ggagggaatt
    16081 ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt tatgggctgg
    16141 atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg
    16201 tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag tactatctgc
    16261 caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg ctaggggagt
    16321 ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta ttatgttgca
    16381 gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg gctatctatg
    16441 acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta attgtatggt
    16501 tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt ttcagtatga
    16561 tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt tttaaattag
    16621 cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacactg ctgttgaaca
    16681 tgattatatt tgtattattc atttttccct ttaagaaatt attcagtgat gaataatttc
    16741 tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga aatgctagac
    16801 ttaacgcggc tttttcatga tcattttctg aaacgaacag ttttgtcatt tttaaaagtt
    16861 gttttgaaac atttttgtaa cataactatt atatcgttga cacactgcta tggtacaatt
    16921 tttatatcgt taagaaagac ttaaatatat ttagatattt attaaattaa gccagtcgga
    16981 ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa tgaccttgac
    17041 tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt gattctcaga tacaacagca
    17101 agatcaaaag attgcagact tgaaaaatca acaagcagat gcacaatcac aaatcgatgc
    17161 cttagaaagc caagtttctg aaatcaacac acaagcacaa gacctattag caaaacaaga
    17221 tacattgcgt caagaatctg cacaattagt aaaagacatt gcagatttac aagaacgtat
    17281 tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa gcaacacaag
    17341 ctcaaactac atcgatgctg tattgaatgc tgattcatta gcagatgcta ttggacgcgt
    17401 tcaagcaatg acaacgatgg taaaagcaaa caacgacttg atggaacaac aaaaacaaga
    17461 taaaaaagct gtagaagaca aaaaagctga aaacgatgct aaactaaaag aattagctga
    17521 aaaccaagct gctttggaat ctcaaaaagg cgatctttta tctaaacaag ctgatttgaa
    17581 cgtactgaaa acttcattag ctgctgaaca agcaactgct gaagacaaga aagctgactt
    17641 gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac aacaacgtct
    17701 agcagaacaa gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag cacgcgaaca
    17761 agcagaggct gaagcacaag ctactcaagc ttcatctact gcacaaagct cagcaacaga
    17821 agaatcatca gctacacaat cttcaatgac tgaagaatca tcatcagcta cacaatcttc
    17881 agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag cgcctgaatc
    17941 ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca acaacagaag aatcaacaac
    18001 acctgcgcct acaacaccat caacagatca aagtgttgat acaggaaacg gcacaggaag
    18061 tagtactccg gctccaacgc caacaccaac acctgaacaa ccaaaacctg taacacctgc
    18121 tccagctcct tctggaagtg tgaatggtgc agcaatcgta gcagaagctt acaaatatat
    18181 tggtactcca tatgtttggg gcggtaaaga tccaagtggc tttgactgct cagggttcac
    18241 acgctatgtt tacatgcaag taactggtcg tgacatcggt ggttggacag ttcctcaaga
    18301 atcagctgga acaaaaattt cagtttctca agcaaaagct ggagacttgt tattctgggg
    18361 gtcacaaggc ggaacttacc acgtagcgat tgctttaggc ggaggacaat atatccatgc
    18421 tcctcaacca ggcgaaagcg taaaagttgg ttcagttcaa tggtttgccc ctgactttgc
    18481 tgtcagcatg taagaatgat taatcaaaaa gagttagccc attctggact aactcttttt
    18541 ttattgtgat aaaatagaaa tcagataaat tcttctgtca attccatgaa cgtttggata
    18601 tcttcagtca ccatgtcgat acctgcttgc caaaaatctg gttgggtaag atcgacattc
    18661 aaatgtttct tcgcaagatc ttccgtcgtc attgaggcag tatcgcgtag taaatggata
    18721 tattcttctt caaaatttgc tcccttttgg ttggcgtaag catagatgcc caaactaaag
    18781 aggtagccaa atgtgtatgg gaagttataa aacggtacgt catcaataaa gaaatgcaat
    18841 tttgcagccc agaaatatgg atggtaagta gctaaagctc cttgatagcc ttctttttgc
    18901 gcctcaacca tcatctctgt gatcttatct tctgagacta atccttcttg ccgtgcttca
    18961 tagaatcgat tttcaaaaat gaatcgggaa tggatattca tgaacatagc taaagcattt
    19021 tgcagttttg tatctagcaa attgattttt tcttcttttg tttttgcgac ttttaatgta
    19081 gcatcggcta cgatcagttc tgcgaatgta cttgctgttt ctgcgacatt catggcatat
    19141 tcgcgattta aagaaggtaa atcccacatc gtgctgctat ggaaagcatg tcctagttca
    19201 tgagcaagtg tcgcaacctc attaacagaa ttactgtagg tcataaagat ccttgattct
    19261 tttgtttctg gtaactcagt acaataacca ccaggacgtt tcccaggacg atcttctgct
    19321 tcgatccagc tcttttcaaa agcagattgg gcaaatttag ccatttttgg actgaatttg
    19381 ttaaagtttt cgataataaa tgctgctgct tcgtcaaaac taaaggtttt ttctttcaaa
    19441 tcgcctaaaa taataggtgc atcttgatct tgccattcca ttttttcttt accgaaaaga
    19501 ttagcttttc tagtcaagta attaacaagg ggttgtttgt ttttctggat tgtatcccac
    19561 atgacagaca atgtttcttt cttcaaccga ttgtactcta atggtttttg tagaaaatct
    19621 gtgacaccat gaagttcata gtcagaaagt ctaaaaccat ctaaatgatt caaagtatcc
    19681 gtgaagaggg gtgccttttc tttccaggct tcttcccatg ctgcgaaaag tgtttctctg
    19741 acttttggat ctggatcgcc catcatctta ttgaaggctt gtccagcaga taattcgact
    19801 acttgtccat cttgttcgaa gggaatcgaa atgctggcta caatcgtatc ataatgactg
    19861 ctccaagcat ttagaccatc taaagaaagc gtatttataa tgttttcttc agcttctgat
    19921 aataattgtg agccatcacg acgaatctcg tttaaacgaa aagcaattgt ttcaaacgaa
    19981 gattgagaaa gcatctgatt ccaatcattg tcagatattt ctgcaaattt ctttgacaat
    20041 acagtttcgg ctgattgcag acgaggcagc attgcgtaaa gcttgcctga taagaccttt
    20101 gcatctgaat cattgacatt ggcagaaagc aatgcagtaa tatagctgct gcattgggta
    20161 aaaccattcg taatcgtttc ttgcaattga agaatagcgt ttaactgctc tgcattgtca
    20221 gaactaggtg accattttgt tacgcggtga taatactcgt tcatttggtc ttccaattgc
    20281 ttcatccgtt ggttcaaagc atcagaatga ctgcctccgg aaaaaataga atctagattc
    20341 caatttaatg aataactcat gaaaaaactc ccttcatatt gtaatacaaa attaagtata
    20401 gcactttttt atggaagaag tctctatgaa aatgattgtt gtttcttcaa aaaaagtcta
    20461 agataaaata cagaggtgac ttatgaattc ttcaaaaaaa caaatgaaaa acggaaatat
    20521 ttatttagta atcgcactga tagtcgttat aatcttattt tacagttctt ctcaaactta
    20581 cggacagcaa tcacaagttt cgcgattaga aagctggctg cctaatcagc catttaaaga
    20641 cacattagcg ggtatacgtt ttaattatgg tggaagtatg gtgagtatca accatctggg
    20701 ctacgccaaa tttttagagt tctttttaag gaagggcgca catttcggta cctattttat
    20761 tttgggaggc agcttgtttt taggtgtttt ccccaagcta aaaatttggt ggctgacagc
    20821 gatccttgct tggctttcag caacagggta cgcaggtttg gacgaattcc atcagatgat
    20881 gaccggtgga cgaacaccta tgttccaaga tgtcatgttg gattcgatcg gtgctctgac
    20941 tgctgtgata ttatgtgtgc tttttgcggc attcaagaag cgtttataga ttggctgtgg
    21001 ctttgttttt ttgtgaaata gtactagaat agaagtgtat tctcaaaaga acaagggagg
    21061 aaaaatcatg tcaggacata gtaaatggca taatatccaa ggaagaaaaa atgcccaaga
    21121 tgcaaaacga gggaaagtct ttcagaaact ttctcgtgaa atttatatgg cagctaaagc
    21181 aggcggtcct gatccatcta tgaatccagc tttgcggctt gttgtggaca aagcaaaagc
    21241 tgcaaatatg cctaacgaca atgtggaacg ggcgattaaa aaggctacga gtgccgtaga
    21301 tgaaacgaat tacgatgaaa tcacgtatga aggatatggt ccgggtggag taggaatctt
    21361 agtccatgca ttgacagata accgtaatcg gacagcgaca aatgtccgag tagcttttac
    21421 tcgaaacggt ggatcactgg gagaaacggg ttcagtcagt tacttgtttg accgcaaagg
    21481 atatatcgct attgaacgcg atgggttgac tgttgatgaa gagacgatgt ttgaggatgt
    21541 attagaggcg ggggcagaag atctgcaaac ttctcctgaa gtattcgaga tttatactgc
    21601 accagaagat tttgctgctg tacgagatga gttggaaaaa gagttcactt tggcgcaagc
    21661 agaattgacg atgattccac aaacgacgat tgatttaaat gaggaacaaa aagagcaatt
    21721 ggaacgtcta gtagataagc tagaagacga tgatgatgtt tcagaagtct ttactgctgc
    21781 tgaaatgtaa gtagcacatt gttagataat aaaaagaaag cacctgtacc aatcgtacgg
    21841 gtgctttctt tttatatatt atttttgtta gaagaccaat acaggcgttc ctttttcgac
    21901 catcccgaat aattccttca ttacacttgg tggggtgttg atgcagccgt gtgaaccgcg
    21961 ggttttccat aagtctccac cgtattcagg ttgccagtct gaatcgtgga taccaacacc
    22021 tgtccagtca atcggcatcc agtagtttac cggactctcg taaggtgttc catcgtcatt
    22081 tgttcctttt aaagttgcat cttcttcttt attccaaaca tagaagactc cagcaggtgt
    22141 aggtgtagtt ggctttccgg aaacgatatc tgtttcaagg gccacttttc cgtctttgta
    22201 ataccacata tgctggttct ccagatcaac ttcgatatac gtatcttcga tcaatggatg
    22261 atcagcggtt gtgccacctt gcacaatggg tgaacgagta aaatcttggc ctgccagaat
    22321 cgcttttttc aatgcttctg tctcactgtc tgtttggatc gtccaactgt aagtgccaac
    22381 tgggaccgta acttctcctc tttttgtact tttaaatttt gtgtcattcg tacttgtgtt
    22441 atactttgtg cctaaatctg ttacatattg acgtacttgt tctgtatcta aatcgacttt
    22501 tccatcattg taggtcagcc aactcataat atcacttgat gggatctgga aagtctcacc
    22561 gtttatactg taagtagctt ttacatttgc gatagcattc atggatgcga gttgttcttt
    22621 cagtgagctg tcttcagatg ttacttttgg tttttctttg aattcagtca attcaattgt
    22681 atctttacca gagtttaccg cactttttaa ttgtttgacc gcagcatcga cgtcgatcgt
    22741 atttccattg acttcaggct tgataacgaa agagtcacct gctttttcga tcgttgcatc
    22801 agtagtaggt gtccggtctt tgttcaaatc agtcagctta gttgtcaatg tctgaacggt
    22861 agtgtctagt ttttggtggt tttgactgac aggatctatt tcttgttttt ctgccgctga
    22921 gacataagtc attccccaag tccatccgtt ttgttcattt aggatcggag aaagctccga
    22981 agtgaaatca tctttgtagc cgagctcgga ttttggtact tcctgccaga cttttccatc
    23041 ttctttaata gccagttttt tatcagaata agcttctttt aatttttcat ttgctttttc
    23101 gactgttaga ttgctgacat ttattccatt tatttctgta ttcggcaaaa aatgctttgt
    23161 gtagtatgta ctgcgatagg tataaaagct gatcagcact aagagaacac caagaactga
    23221 aagcaaagca acatgtttcg atttttttcg atgcgaagat cttgtcatat cgacaactcc
    23281 ttaagtaaaa gtttagggtg gaacggtctc atgatagcat aatttagagg aaaagacgaa
    23341 ggttatctct actttatgaa aaaatttaga aaccataagg gtgataaaat aaaaaaacgg
    23401 aaaacaccga atagatgttt tccgtcatgt tcaaagatta ttcagcgtct agtttagcaa
    23461 aagcttcatc tagaatttct tttaattgct tagcagaagc agccatacgg tcttgctcgc
    23521 tgtcacttaa aggaatttcg attacttttt ggataccttg acggttgata acagcaggtg
    23581 caccaatgta gatgtcgttc aatccatatt cgccattcat gaatactgat aaaggaagaa
    23641 cagcattttc atcatctaag atggcacgag tgatacgtgc taatgctact gcgataccat
    23701 agaaagtagc gccttttttc tcgataattg tgtaagcagc gtcacgtacg ccaaagaata
    23761 ggttaaccat agcttcttca tcgatttctg ggttgttttt tacccattca tagatttgca
    23821 agccggcaac atttgcatgt gaccaaactg ggaattctga atctccgtgt tcacctaaga
    23881 tgtaagcatg gacgttacgt gcatcaacgt caactaattc cgcaagtgct tgacggaaac
    23941 gagctgagtc aagagaggta cctgaaccga ttacgcgttc tttagggaaa cctgagaatt
    24001 tccaagttga gtaagtcaaa atatctactg ggttagcggc aactaaaaag ataccgttaa
    24061 atcctgattc aacgatttgt gtgacaacat cgcggttgat ttttaggttt ttgtttacta
    24121 agtctaaacg agtttcacct ggtttttgtg gtgcgccagc tgtaataaca actaagtcag
    24181 catcatgtgc gtctgcgtaa gtagcagaat agattttttt aggtgaagta aatgccaaag
    24241 cgtgagacaa gtcaattgcg tctccttcag ttttagcagt attgatatcc acaataccta
    24301 cttcttgagc gatattttga gtaactagag caaaggcata gctagaacct acggcgccgt
    24361 ctccaacaag aataactttt tgatgatctt ttttttctgc agctgcagtc atttagtatc
    24421 aatccttcca ttgatttgtt ttttcccatg cttatgctaa catttctcaa gccccatgtc
    24481 acgctatttg gctataaaat ataacagata acaaggttgt attatatgta aaagcttaca
    24541 tgtg
//
LOCUS       DADOOS010000028        23829 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.028, whole genome shotgun sequence.
ACCESSION   DADOOS010000028 DADOOS010000000
VERSION     DADOOS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.028"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(54..788)
                     /locus_tag="J8C37_000958"
     CDS             complement(54..788)
                     /locus_tag="J8C37_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAZ5304417.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="J8C37_000959"
     CDS             1115..1525
                     /locus_tag="J8C37_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAZ5304418.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="J8C37_000960"
     CDS             1518..2219
                     /locus_tag="J8C37_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAZ5304419.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="J8C37_000961"
     CDS             2219..3805
                     /locus_tag="J8C37_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ5304420.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="J8C37_000962"
     CDS             3824..4045
                     /locus_tag="J8C37_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAZ5304421.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="J8C37_000963"
     CDS             4042..4803
                     /locus_tag="J8C37_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAZ5304422.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="J8C37_000964"
     CDS             5183..5692
                     /locus_tag="J8C37_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAZ5304423.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="J8C37_000965"
     CDS             complement(5767..6306)
                     /locus_tag="J8C37_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAZ5304424.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="J8C37_000966"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="J8C37_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAZ5304425.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="J8C37_000967"
     CDS             7373..8170
                     /locus_tag="J8C37_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAZ5304426.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="J8C37_000968"
     CDS             8198..8833
                     /locus_tag="J8C37_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAZ5304427.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="J8C37_000969"
     CDS             8853..9626
                     /locus_tag="J8C37_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5304428.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="J8C37_000970"
     CDS             10034..10831
                     /locus_tag="J8C37_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAZ5304429.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="J8C37_000971"
     CDS             10809..11222
                     /locus_tag="J8C37_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304430.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="J8C37_000972"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="J8C37_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAZ5304431.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="J8C37_000973"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="J8C37_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAZ5304432.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="J8C37_000974"
     CDS             15999..16559
                     /locus_tag="J8C37_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAZ5304433.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="J8C37_000975"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="J8C37_000975"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAZ5304434.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="J8C37_000976"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="J8C37_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAZ5304435.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="J8C37_000977"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="J8C37_000977"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAZ5304436.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="J8C37_000978"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="J8C37_000978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAZ5304437.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="J8C37_000979"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="J8C37_000979"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAZ5304438.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="J8C37_000980"
     CDS             21906..22484
                     /locus_tag="J8C37_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAZ5304439.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="J8C37_000981"
     CDS             22536..23018
                     /locus_tag="J8C37_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAZ5304440.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="J8C37_000982"
     CDS             23135..23509
                     /locus_tag="J8C37_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304441.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6648 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagttt
//
LOCUS       DADOOS010000029        23165 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.029, whole genome shotgun sequence.
ACCESSION   DADOOS010000029 DADOOS010000000
VERSION     DADOOS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.029"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(23..1174)
                     /gene="queG"
                     /locus_tag="J8C37_000983"
     CDS             complement(23..1174)
                     /gene="queG"
                     /locus_tag="J8C37_000983"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAZ5304442.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1193..1945)
                     /locus_tag="J8C37_000984"
     CDS             complement(1193..1945)
                     /locus_tag="J8C37_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304443.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(2086..3036)
                     /locus_tag="J8C37_000985"
     CDS             complement(2086..3036)
                     /locus_tag="J8C37_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304444.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(3037..3876)
                     /locus_tag="J8C37_000986"
     CDS             complement(3037..3876)
                     /locus_tag="J8C37_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAZ5304445.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(3873..4619)
                     /locus_tag="J8C37_000987"
     CDS             complement(3873..4619)
                     /locus_tag="J8C37_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304446.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            4857..6227
                     /locus_tag="J8C37_000988"
     CDS             4857..6227
                     /locus_tag="J8C37_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5304447.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6282..7178)
                     /locus_tag="J8C37_000989"
     CDS             complement(6282..7178)
                     /locus_tag="J8C37_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5304448.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7459..8136)
                     /locus_tag="J8C37_000990"
     CDS             complement(7459..8136)
                     /locus_tag="J8C37_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ5304449.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8227..9132)
                     /locus_tag="J8C37_000991"
     CDS             complement(8227..9132)
                     /locus_tag="J8C37_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ5304450.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9307..10761
                     /locus_tag="J8C37_000992"
     CDS             9307..10761
                     /locus_tag="J8C37_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5304451.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            10777..11808
                     /locus_tag="J8C37_000993"
     CDS             10777..11808
                     /locus_tag="J8C37_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAZ5304452.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11815..13002
                     /locus_tag="J8C37_000994"
     CDS             11815..13002
                     /locus_tag="J8C37_000994"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAZ5304453.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13158..14540
                     /locus_tag="J8C37_000995"
     CDS             13158..14540
                     /locus_tag="J8C37_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAZ5304454.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14512..14898
                     /locus_tag="J8C37_000996"
     CDS             14512..14898
                     /locus_tag="J8C37_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304455.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15098..15766)
                     /locus_tag="J8C37_000997"
     CDS             complement(15098..15766)
                     /locus_tag="J8C37_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAZ5304456.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(15955..17381)
                     /locus_tag="J8C37_000998"
     CDS             complement(join(15955..16808,16808..17381))
                     /locus_tag="J8C37_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ5304457.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            17624..18079
                     /gene="tnpA"
                     /locus_tag="J8C37_000999"
     CDS             17624..18079
                     /gene="tnpA"
                     /locus_tag="J8C37_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAZ5304458.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            18344..18442
                     /locus_tag="J8C37_001000"
                     /pseudo
     CDS             18344..18442
                     /locus_tag="J8C37_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(18665..18895)
                     /locus_tag="J8C37_001001"
     CDS             complement(18665..18895)
                     /locus_tag="J8C37_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAZ5304459.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(19125..19943)
                     /locus_tag="J8C37_001002"
     CDS             complement(19125..19943)
                     /locus_tag="J8C37_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ5304460.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            20104..20793
                     /locus_tag="J8C37_001003"
     CDS             20104..20793
                     /locus_tag="J8C37_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAZ5304461.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            20807..22309
                     /locus_tag="J8C37_001004"
     CDS             20807..22309
                     /locus_tag="J8C37_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5304462.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            22322..22798
                     /locus_tag="J8C37_001005"
     CDS             22322..22798
                     /locus_tag="J8C37_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAZ5304463.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(22895..23005)
                     /locus_tag="J8C37_001006"
                     /pseudo
     CDS             complement(22895..23005)
                     /locus_tag="J8C37_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6899 a         4356 c         4300 g         7610 t
ORIGIN      
        1 ttgaataatg ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt
       61 ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa
      121 gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga
      181 tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta
      241 gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag
      301 ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt
      361 ttggatatac ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt
      421 gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt
      481 tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg
      541 cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac
      601 agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct
      661 caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa
      721 atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg
      781 gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga
      841 tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag
      901 caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa
      961 gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat
     1021 cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt
     1081 cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt
     1141 tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc
     1201 tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg
     1261 caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg
     1321 aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac
     1381 ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat
     1441 acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc
     1501 caggcctagc aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa
     1561 taaattgact tttataatag gccgactgga tctttttgat tactccttct gctttgatga
     1621 ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc
     1681 caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta
     1741 aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg
     1801 tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt
     1861 gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat
     1921 atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg
     1981 aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct
     2041 tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt
     2101 ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc
     2161 tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc
     2221 cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac
     2281 cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg
     2341 actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata
     2401 cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact
     2461 ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg catttttttt
     2521 atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt cctccacata
     2581 ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat gttcttcacc
     2641 gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt ttccttcttc
     2701 ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa gattcaaacc
     2761 gttgtataaa ataacatctg catcgcttgc ctttttgatg tcttctggta gtacttcata
     2821 ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat ctgttccgac
     2881 ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact tttctttact
     2941 tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca agaaactaac
     3001 tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt tctgaaataa
     3061 aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac atgctaacac
     3121 gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga caactgcact
     3181 tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg ttagcaagta
     3241 agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta ctgtctgtaa
     3301 actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt
     3361 cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa
     3421 gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca aatcgctttc
     3481 cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac tctgggcata
     3541 tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc
     3601 atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta gcatcccaaa
     3661 agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta cacctggaag
     3721 aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa taaaagaacc
     3781 gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg
     3841 ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc ttttactaaa
     3901 tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac ttcttgtact
     3961 ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata ttcttctact
     4021 ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag
     4081 atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc atctaaaaag
     4141 atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag ctgtccaccc
     4201 gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt caaagcttgt
     4261 cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt
     4321 cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc acttcgctgt
     4381 tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa ggtgacttta
     4441 ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt tcctgcaccg
     4501 tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat agttttcaaa
     4561 acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt acgttccaaa
     4621 tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta actaaaatgg
     4681 aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg cattttatga
     4741 aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat gaaaaacatg
     4801 tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg
     4861 aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc gcactaggtg
     4921 gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg acagggccct
     4981 cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg cgtgctttag
     5041 gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg agcgagtaca
     5101 tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat attgtcgtag
     5161 gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct gatttgccgg
     5221 caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca
     5281 aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg atcattttga
     5341 tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt caggcagtag
     5401 ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa ggatttttct
     5461 ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga atgactgctg
     5521 gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact atcggtcgaa
     5581 tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg tggaatcaat
     5641 tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt acatttgctg
     5701 caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat tcagggattt
     5761 tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct aaaaaatttc
     5821 tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca ggtggaatct
     5881 taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca gaaaatattt
     5941 tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta gtgattttga
     6001 tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat ccttttaaaa
     6061 tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc gtattgattt
     6121 tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt ttcttgcttt
     6181 acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag
     6241 cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa ttttagcgtc
     6301 tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa gatcgtttct
     6361 actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa aactacggtt
     6421 gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt tgtgtcttgt
     6481 gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga atcagaagat
     6541 gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg caaataaata
     6601 tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat caaagtatct
     6661 aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt tctgatccct
     6721 tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga taaaccagct
     6781 attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc aggcgtcttt
     6841 tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg caaaccattt
     6901 gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc acttgtttgt
     6961 ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa
     7021 ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa cacggcaact
     7081 gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc
     7141 atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac caatttttat
     7201 gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg tcgctgtgca
     7261 catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc caagattgtc
     7321 cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc agtttcttta
     7381 tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca tctaattgtt
     7441 ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa
     7501 gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt gtttttcaga
     7561 ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt caatccgctg
     7621 agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta gcttctccca
     7681 acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa gataggtcat
     7741 atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg tatattcttc
     7801 ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga ttcctagttc
     7861 tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa tttttaaatg
     7921 agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat gctgggccaa
     7981 gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt ccaaatgatg
     8041 ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta aagattcttc
     8101 gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt tttagtcttc
     8161 gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc attttaacca
     8221 gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt tcaaggtatc
     8281 caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg tagccgtttc
     8341 tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc ctacttgata
     8401 ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc gattgatccg
     8461 taccccgatc aaatattcct tgatactggt acccatatga tttttgaata ctcgcgacaa
     8521 atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt tttcagcata
     8581 atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct tttgtacacc
     8641 accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg ttaatgctgt
     8701 taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta aaataatgtc
     8761 gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga aaagcgactg
     8821 ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca tccacggatc
     8881 ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca gatcaccttt
     8941 tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca tcacgacatg
     9001 caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg ttttagagaa
     9061 accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt ttaaaaaaat
     9121 cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa aagttaaata
     9181 aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag tcattaaaag
     9241 atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag gagaggaata
     9301 cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc ttttggacac
     9361 gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag ccagatgttc
     9421 gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag cttagttgta
     9481 ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat cgataatact
     9541 cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt tagttccgta
     9601 ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac gactttattt
     9661 atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt taaggatatc
     9721 gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga aaaatttgct
     9781 acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt cgttgtccct
     9841 atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc ttcaggctgg
     9901 ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat cactgccttg
     9961 ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat
    10021 gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct
    10081 tatgcgattg ccaatgtagc aacaactgcc gtattattct acttcttcaa atatgtactt
    10141 ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg tttagtagct
    10201 gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta ccttggaggg
    10261 atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag tttgccaatc
    10321 gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt atccgctttg
    10381 atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg taacgaagct
    10441 gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc taacgggatc
    10501 gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc tgatatcact
    10561 gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc tttgatgatt
    10621 ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca tgcacaaatc
    10681 gttgaggaat tggaaaacaa attggctcca gctgatacaa tggaagatga acttcacgat
    10741 gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga aaaagatttt
    10801 ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact ttctatctct
    10861 gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt tttgggattt
    10921 gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc
    10981 gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca attagccaca
    11041 gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg
    11101 tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac tacaagtccc
    11161 gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac tttgacaaaa
    11221 gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt attcaatccg
    11281 acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga tcaacatgag
    11341 ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc tataggggta
    11401 aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt
    11461 tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc atttttctta
    11521 aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa aagaatccaa
    11581 gtaggtattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt cggcacagaa
    11641 acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt tgagactcaa
    11701 acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa agctggatca
    11761 gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact catcatggaa
    11821 atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa aactacacaa
    11881 tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa tggaggccat
    11941 gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg agacgataat
    12001 aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt tactttagat
    12061 gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat ggtccttaaa
    12121 ttaaaagaag ctggacactc aatcgatcat gggtttgagt tgttagtgga aggaacaatc
    12181 cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg tgttgtatta
    12241 gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg taaaaaagta
    12301 gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat cggcttcggt
    12361 gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat ggttccagtg
    12421 aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga attggcagat
    12481 tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact
    12541 ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac tattgaccaa
    12601 atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa tcagcggact
    12661 ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca actgttaaat
    12721 gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt agatacacta
    12781 gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg agctggattt
    12841 ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc
    12901 tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc acacattgga
    12961 aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa tcaacgatct
    13021 cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg agccttttgc
    13081 tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt tctaattgtg
    13141 ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat gaaaaagaac
    13201 cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta gagtgcgttg
    13261 aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg tccgattcga
    13321 cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg
    13381 gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt gatttatata
    13441 ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat tctatctata
    13501 aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc ttccttgatg
    13561 taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct aaactgatcc
    13621 ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc gataacccgc
    13681 tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg tttgctgaat
    13741 ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact gacttttggg
    13801 gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct gtttatgatt
    13861 tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt actcagcttt
    13921 atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca ccaaaatcaa
    13981 aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa gaaatcgaga
    14041 tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa
    14101 cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca aatggcaaga
    14161 ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat ctagcagatg
    14221 ctttattgac cattttccac cagcattacc actaccagga tattcggcgt gtaggcgtga
    14281 actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa
    14341 agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa aaatacggct
    14401 ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc gcacgtagtt
    14461 ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa gatgagacgg
    14521 acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg attaaaatgg
    14581 gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga agaagaagca
    14641 cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag tatactgaca
    14701 gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga tgaatttgga
    14761 agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga ttactttatg
    14821 attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa ggaaacaaaa
    14881 aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa ataagaaagg
    14941 atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt cgaagaagca
    15001 acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt agttttgagt
    15061 aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt cggtaagatt
    15121 catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac cattcaacat
    15181 aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata agccatgctt
    15241 cagtcaattg attggactga agcactttat gctgaatttg cccttcatct acaagcagga
    15301 tcgataaagc tttgtcttca tctgattttt tcaatacact catttgacca gcattctcta
    15361 ggataacatg tttggcattt tcaagagaaa agcattcttg ctgtcttaag attgctcgca
    15421 actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg ccgtctttga
    15481 ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt tggacgatac
    15541 gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca tgccctacat
    15601 ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc gtatagacta
    15661 aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc actaataata
    15721 aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt tctcccccta
    15781 actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat atacaaggaa
    15841 gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc aaaagttaga
    15901 cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat atttttacac
    15961 aaccgttacg gttgttttac gaaattggac gagactttgc cagtttaatt tctgttttat
    16021 ccgttcatta ttgtagtaat agatatagct atcgatggct gattttaatt cctcgaaaca
    16081 gttataaact ttcccgtgaa aaatctcttg ttttaataag ccaaaaaaat tctccataag
    16141 tgaattatct aagcagttcc ctttccgaga catgctttga aagattttgt actccttcaa
    16201 tttgtttcta taagctttca tttgatacgc ccaaccttga tctgagtgaa acgtacgacg
    16261 atagggacaa tcgttagtgg tctggatagc ttcctccaaa ccttccatta ccgctaatgc
    16321 attgggcttt tcagagatac gataggataa aatctcacta ttgaacatat ccataaaagg
    16381 atctagatac aatttctttt gacggataat gcctttggca tccacttcat ggtatttaaa
    16441 ttcagttgta tccgtcgtga ttttctgatg gcagatactg gtgtagaaac gacgatggat
    16501 ccgatttttg gctactgctc cgacttgtcc tcgataagaa ttgtagcgcc gtgatttacg
    16561 tgtgtatgtc gttactttta ttcctagctt cttgatcagg cgctgaactt tctttttatt
    16621 gatagagaaa ccgcggtttc ttagttcatt ggtcatccgt aaacagccat aatccttgtg
    16681 cttctcccga actttaagca tttccgcttc aatctcttga tctggatttt tacggtccaa
    16741 tcgtttttgc cagtacataa agattgcctt agggaacttt aaagtttcta gaagctcaac
    16801 taatttaaac ggtcctcgga ggcttgcgat gattcgtgcg attgcttcat tcgtcgttgt
    16861 tgggcttcct gttttcgcag cttcctcaat tcttttaaaa aggcattttc aatttgaagg
    16921 gaacggactc gtttctccaa ctcctcgatg cgttcacgtt ctttagacgt tgctttagtt
    16981 gtagattttt tcttattttt gggttcattt ttcttagaca agataggtgg acatcctttc
    17041 gtttttgaga gtccatcgat cccaccctca cggaattgac gcatccaact agcgatcaaa
    17101 ttagggttgt tcatgttaag tgtgttggca acttcacgat aggacagctc acttgttaga
    17161 tataactcta tcgcatccag cttaaattga acagaatatt ttttcttttg tcgctttctt
    17221 actagaccct cttcaccaaa ttcttgatag gcattgatcc agcgcttcac ttgtgatgga
    17281 gccttaatgg aatatttctt tgccagataa tcacttccgc ctttaccatc caagtaatca
    17341 tgaacgatct ttaatttaag ttcaaaagta tacttagcca taaaaaacac cgacctccaa
    17401 aagttagatt ttttggtcta acttttgggg gtcggtacaa aacttaaaag ttttagaccc
    17461 ttttattttc ttaatgactt cagtcagttt tgtgcgtaaa gcgtgcgaaa caagaaaacc
    17521 gcgcgtttct acgcgtggaa gaagtatctt tagcgataaa aactcctttc gatatactag
    17581 tgagtggcta ccaaccgctc atcagatcga aaggagtttt ttcatgtcaa atgacgataa
    17641 aagtttagca tatactcgtt ggaattgtaa gtatcatatt atttttacac caaaatatca
    17701 acgaaaagta atctatgggc aattacgaaa agatcttgga aaaatactta ggaagttgtg
    17761 cgaaatgaaa gatgtggaaa tcatagaagc acatgcaatg ctagatcata ttcacatgct
    17821 tgttcgagta ccaccaaaaa tatgtatatc cagttttatg gggtatctga aaggtcgtag
    17881 tgcggtacta atccacgagc agcacgcaaa tctcaaatat aaatacggaa acaaatcctt
    17941 ttggtcgaag agatactatg tgagtacagt cggattgaat caaaagacaa atgaaaaata
    18001 catccgtgaa caagaagctg atgatcgcat tcgatatatt ataagtaaaa gagagtacgt
    18061 agaaccattt aaaaaataaa gagaagtaag tgagtgaaga tgagcagttg gcggtctttt
    18121 aaaaatcccc ttttaagggg cagcgagtgt cgaccccttc tagggggatt aaaaatacac
    18181 ccgtcctcac gggtgtatgg gttactgata gacttgattt taactggtgt cagaaactta
    18241 tgtccagaaa gggtgatgaa attttggcaa tttacggcta ttcaagaatt agcacaagca
    18301 accaagatta caaaacacaa attcaaacat tagaaaatgc tggcgcagaa ataatcttcc
    18361 tagaaaatca ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga
    18421 tgatctgtct aacttaattt gacaattaag agtatattta cttcagatgg taaggcaaca
    18481 aaataaaata caataggcag atgaactaat aaataaaatc tgtattaaat acagataaat
    18541 taatataaaa atcaaaagga tcataaactt ttaacaagtg atgaaatggg tggaaattga
    18601 ttcttataga cagtttgtct cataacattg aagtattttg caattttagc attgaaacac
    18661 ttttttaatc ttcaacgttg ttcttataaa caagatgatt gggaatcttt tgaattaaca
    18721 ctgtatagct tagaacaccc tttatagaaa acattcgctt agcaatctta ataattcctt
    18781 gtgcccgatg tcgtttagat tcagtaggtt cctgctcatc gatcatggaa aaaatttgaa
    18841 ggatcagatc cttaataaat ttatttaaga gaggatgatc cattgcttca tccatactcg
    18901 ataaactagt gataatgagc tacacgtttt tctttcaagt tgatcaatag cttccacgat
    18961 aaaaatatct ttagataaaa tcaagaaccg taggaactac agggatattt ggtcaataag
    19021 agccaccgct attagtaaaa aataagctat caagtatgct ctcatcccaa gaattgccta
    19081 cttcaatcgt taaaaaccgt taggtttagc taagtggggg tagttcactc aatactttta
    19141 tcaataatag agcgcaaaac tttttctctt aattcaactg aatcaatatt attgttttgt
    19201 tcataaccat gaactaatag atcacaaata taaagttgtg atatttttcc agctaaagat
    19261 ccaccattaa gaaattcttc aatggctgtt tgtaaaacta agtctgcaga ctttcctata
    19321 ggtgaatgaa tatagttagt tatagcaata atttttgcgc catttttttt agctattttc
    19381 aatgaatcat aagtatcttt cgttttccca gataaagaaa aaatgattac taaatcatta
    19441 actgttaata aagaagcgac ttgggcttga aaatgagggt ctagtacagc ttttgcttga
    19501 actcctacgc gaagaaacat attttctaaa tcaagactag tatttccact agatcccact
    19561 ccaaatatat atatgttttt tgattgagtg atcattttaa tggcttcatt caatttttct
    19621 tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta aactattagc aatttcatca
    19681 tagtacttta cactagtcgg atgttctttt ttttgactaa agtcttcctt tgcaatttca
    19741 atttttaaat cagaaaaacc agaaaaacca attttttgac aaaagcgaat aatagtagca
    19801 tcgccaactt ttgtgttttt cttaatatca ctcatcgtac tataaataat agttttccca
    19861 gaatttaaaa taaaatcagc gacttttttt tcagacttcg ttaaaaatgg ataattttct
    19921 ttaattaaat agatataact catacaaact cctctacaat ttcaactttt ctccattata
    19981 ctccaaaata ataaaaaaaa ctcctaaata ctccattttt atctttactt ttatgaaagc
    20041 ggatgctata ttgtttttgg agttgttatc caaaaataat aaaaaattgg agtgtttagg
    20101 ataatggata aaaagaggtt tttagatctt atttcaaaag gtttgattgt ttcctgtcaa
    20161 gcattacctg gtgaaccatt ttacaaagag gaagaaagtt taatgcctta ttttgctttg
    20221 gctgctcaaa gggctggagc agttggtata cgtgcaaatt ctgttcgaga tattattgaa
    20281 attagaaagt gtgtatcatt accaatgatt ggaattatta aaagagatta tccaccagaa
    20341 tctccattca ttactgccac tatgagagaa gtagatgaat tagtagaaac cggtgttgag
    20401 gtaatcgctc ttgattgcac tcttagaaat cgacatgatg gactatctat aaattctttt
    20461 attactaaaa taaaagaaaa atatcctcat cagttattta tggcagatat ttctacattt
    20521 gaagaaggca aaaatgcata taaagcagga atagattttg taggaactac tttaagtgga
    20581 tatactgagg aaagtaaaaa acaagatggg ccagatatag atttgattaa aaaattggta
    20641 gaagaaggga tttgtgtaat tgctgaggga aggattcata ctccagaaca agcaaaagag
    20701 ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg ctattactcg accacaggaa
    20761 attgcatcaa gatttgttaa agcattaagt tagggggaga aataaaatgt ttaaaaaatt
    20821 ttcacaatta ggacgcgctt tcatgttacc aattgctata ttaccagtag ccggattact
    20881 attgggactg ggaggagcgt taacaaatga gagtgctata aatgcttatc catttttaga
    20941 tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat gctggaaatg cagtttttgc
    21001 aaacctagca ctaatttttg caattggtat tgctgtagga cttgcaaatg gagataaagg
    21061 aacagctggt ttagctggtg gagtagctta tctagtatat actgcaacta ttagtgggtt
    21121 tcttgaactt ttttcagcaa aagatgcaac actagataca ggtgtagtgg gatcaattgt
    21181 tgttggtggt actgttgcat ttttacacaa tcgttataga aaaattgaat taccacaatt
    21241 cttaggtttt tttggaggat caagatttat tccgattgtt tctagtttag ctgcaatcat
    21301 tattggttct ttcttttatt taatttggcc accaattcaa aatggtttag tagttgctgg
    21361 tgaacaaatt gcccaaatgg gtagtttagg aactttttta tatggttttt tattgcgttt
    21421 aacaggtgct gttggactac accacaccat ttaccctttg ttctggtata catctttagg
    21481 tggtaccgaa actgtagctg gatcaacaat tgctggagcc caaaatattt tctttgccca
    21541 actagctgat ccaaaccaca ctggtctgtt tacttatggt actagatttt ttgcaggaag
    21601 atttgcaaca atgatgtttg gattaccagc agcatgttat gcaatgtatc gtgcaattcc
    21661 taagaaaaat cgtaaaaaga atggtgggct ttattttagc ggggccctta catcattttt
    21721 aactggaatt actgaaccag ttgaatatat gtttttattt gttgctcctt ggctttatgt
    21781 gattcatgca ttcttagatg gattatcttt ttattttgct gatatcttaa atattcgtgt
    21841 agggaattcc ttttcaggtg gtttgatcga ctttttatta ttcggtgtac ttcaagggaa
    21901 tgacaaaact aattggttaa aattaattcc ttttggtatt gcatgggcat ttatatattt
    21961 ctttgttttt acattcttta tcaaaaaatt taaggtagct attcctggaa tggaagaaga
    22021 atcagaagtt cattctgaaa aagtaataaa caaaaataca tcattacatg aagagtctct
    22081 tatcattatt gaagctcttg gtggaaaaga aaatattgag tctgttgaag cttgtgcaac
    22141 tcgtttgcgt gttgctgtta cgaatgggga gattattcaa aaacaaacaa taaaagacac
    22201 tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa gctgtttttg gagggaaagc
    22261 tgacttatta agtcaagaaa tcaatcaaat attaggaaaa gataattagg aggtttttaa
    22321 tatgtttgaa attttcaaaa agaaaaaaca agaaactttt tatgcacctt gttccggaaa
    22381 aatcatttcc atcacagaag taaatgatcc tgtcttttca caaaaattac taggtgatgg
    22441 ttttgccgtc atacctgatg gttcaaaaat ttatgctcca ttggctggtc gagtaacaaa
    22501 tatatttcct actaaacatg ctattaatat aaaaagcaac tcaggcattg attatttgat
    22561 tcatattgga attgatacag ttgaattaca aggagagcct ttttcgattt gtgttcaaga
    22621 aaatgatgag gttactcctg aaacctcgtt ggttgatgtg gattttgaca aaataataaa
    22681 atctggtaaa gatacttctg ttatagttgt ttttccggag aaaaatcagt tcaatgattt
    22741 aataatagat aatggtcgaa ttgctcatgg gaattcatgt ggaaaaatca ctttttaaaa
    22801 aagtgtatat aaaaagctat aaaataagct aaataggata tttgtcaaat gaagttggtg
    22861 aacagaaaga actgttttct ttctgttcat ttttttattt tattagtaat gattccccat
    22921 gccaaatcat gatccgtaat ttaaagttat aaaagcttcg aaatccattg gcatttcgtt
    22981 ggaatacctt gatatgatta ttcaaatata aaatatagag agaagagaga gagggaagat
    23041 atttataaag ggcaaagtta tgtttactca acttaggaaa tagaaataga tcaatttata
    23101 ttctcttgtc aatcgaatat gaaccttagc actatatacc aaatatagta aaatgctgaa
    23161 agatg
//
LOCUS       DADOOS010000030        23065 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.030, whole genome shotgun sequence.
ACCESSION   DADOOS010000030 DADOOS010000000
VERSION     DADOOS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.030"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(109..564)
                     /locus_tag="J8C37_001007"
     CDS             complement(109..564)
                     /locus_tag="J8C37_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304464.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="J8C37_001008"
     CDS             complement(718..1926)
                     /locus_tag="J8C37_001008"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAZ5304465.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="J8C37_001009"
     CDS             complement(2038..2370)
                     /locus_tag="J8C37_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAZ5304466.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="J8C37_001010"
     CDS             2518..3393
                     /locus_tag="J8C37_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ5304467.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="J8C37_001011"
     CDS             complement(3475..4056)
                     /locus_tag="J8C37_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAZ5304468.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="J8C37_001012"
     CDS             complement(4062..5321)
                     /locus_tag="J8C37_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAZ5304469.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="J8C37_001013"
     CDS             complement(5323..6369)
                     /locus_tag="J8C37_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304470.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="J8C37_001014"
     CDS             complement(6604..6879)
                     /locus_tag="J8C37_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAZ5304471.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="J8C37_001015"
     CDS             complement(7122..8432)
                     /locus_tag="J8C37_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAZ5304472.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="J8C37_001016"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="J8C37_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAZ5304473.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="J8C37_001017"
     CDS             complement(9978..10658)
                     /locus_tag="J8C37_001017"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAZ5304474.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="J8C37_001018"
     CDS             complement(10707..11342)
                     /locus_tag="J8C37_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5304475.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="J8C37_001019"
     CDS             complement(11408..12811)
                     /locus_tag="J8C37_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAZ5304476.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="J8C37_001020"
     CDS             complement(12798..13829)
                     /locus_tag="J8C37_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304477.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="J8C37_001021"
     CDS             13860..14081
                     /locus_tag="J8C37_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAZ5304478.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="J8C37_001022"
     CDS             complement(14246..14983)
                     /locus_tag="J8C37_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAZ5304479.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="J8C37_001023"
     CDS             complement(15136..16476)
                     /locus_tag="J8C37_001023"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAZ5304480.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="J8C37_001024"
     CDS             complement(16713..18236)
                     /locus_tag="J8C37_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAZ5304481.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="J8C37_001025"
     CDS             complement(18614..19225)
                     /locus_tag="J8C37_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ5304482.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="J8C37_001026"
     CDS             complement(19555..20271)
                     /locus_tag="J8C37_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAZ5304483.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="J8C37_001027"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="J8C37_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAZ5304484.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="J8C37_001028"
     CDS             complement(20830..21627)
                     /locus_tag="J8C37_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAZ5304485.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="J8C37_001029"
     CDS             complement(21597..21968)
                     /locus_tag="J8C37_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAZ5304486.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="J8C37_001030"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="J8C37_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAZ5304487.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DADOOS010000031        22913 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.031, whole genome shotgun sequence.
ACCESSION   DADOOS010000031 DADOOS010000000
VERSION     DADOOS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.031"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            121..345
                     /gene="nrdH"
                     /locus_tag="J8C37_001031"
     CDS             121..345
                     /gene="nrdH"
                     /locus_tag="J8C37_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAZ5304488.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            504..2663
                     /gene="nrdE"
                     /locus_tag="J8C37_001032"
     CDS             504..2663
                     /gene="nrdE"
                     /locus_tag="J8C37_001032"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAZ5304489.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2713..3678
                     /gene="nrdF"
                     /locus_tag="J8C37_001033"
     CDS             2713..3678
                     /gene="nrdF"
                     /locus_tag="J8C37_001033"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAZ5304490.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3767..4906)
                     /locus_tag="J8C37_001034"
     CDS             complement(3767..4906)
                     /locus_tag="J8C37_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ5304491.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5215..5928)
                     /locus_tag="J8C37_001035"
     CDS             complement(5215..5928)
                     /locus_tag="J8C37_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAZ5304492.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6182..6883)
                     /gene="nagB"
                     /locus_tag="J8C37_001036"
     CDS             complement(6182..6883)
                     /gene="nagB"
                     /locus_tag="J8C37_001036"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAZ5304493.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7117..8649
                     /locus_tag="J8C37_001037"
     CDS             7117..8649
                     /locus_tag="J8C37_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ5304494.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8840..10042)
                     /locus_tag="J8C37_001038"
     CDS             complement(8840..10042)
                     /locus_tag="J8C37_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5304495.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10124..10813)
                     /locus_tag="J8C37_001039"
     CDS             complement(10124..10813)
                     /locus_tag="J8C37_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAZ5304496.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10919..11287)
                     /gene="rplL"
                     /locus_tag="J8C37_001040"
     CDS             complement(10919..11287)
                     /gene="rplL"
                     /locus_tag="J8C37_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAZ5304497.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11340..11843)
                     /locus_tag="J8C37_001041"
     CDS             complement(11340..11843)
                     /locus_tag="J8C37_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAZ5304498.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12253..12942)
                     /gene="rplA"
                     /locus_tag="J8C37_001042"
     CDS             complement(12253..12942)
                     /gene="rplA"
                     /locus_tag="J8C37_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAZ5304499.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(13046..13468)
                     /gene="rplK"
                     /locus_tag="J8C37_001043"
     CDS             complement(13046..13468)
                     /gene="rplK"
                     /locus_tag="J8C37_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAZ5304500.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13811..14479
                     /gene="sdaAB"
                     /locus_tag="J8C37_001044"
     CDS             13811..14479
                     /gene="sdaAB"
                     /locus_tag="J8C37_001044"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAZ5304501.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14492..15364
                     /gene="sdaAA"
                     /locus_tag="J8C37_001045"
     CDS             14492..15364
                     /gene="sdaAA"
                     /locus_tag="J8C37_001045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAZ5304502.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15455..16474)
                     /locus_tag="J8C37_001046"
     CDS             complement(15455..16474)
                     /locus_tag="J8C37_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAZ5304503.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGVDGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16647..17534)
                     /locus_tag="J8C37_001047"
     CDS             complement(16647..17534)
                     /locus_tag="J8C37_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ5304504.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17611..18156)
                     /gene="nusG"
                     /locus_tag="J8C37_001048"
     CDS             complement(17611..18156)
                     /gene="nusG"
                     /locus_tag="J8C37_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAZ5304505.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18257..18427)
                     /gene="secE"
                     /locus_tag="J8C37_001049"
     CDS             complement(18257..18427)
                     /gene="secE"
                     /locus_tag="J8C37_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAZ5304506.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18451..18603)
                     /gene="rpmG"
                     /locus_tag="J8C37_001050"
     CDS             complement(18451..18603)
                     /gene="rpmG"
                     /locus_tag="J8C37_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAZ5304507.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18765..19409
                     /locus_tag="J8C37_001051"
     CDS             18765..19409
                     /locus_tag="J8C37_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ5304508.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19465..19968)
                     /locus_tag="J8C37_001052"
     CDS             complement(19465..19968)
                     /locus_tag="J8C37_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAZ5304509.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19981..20027)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20565..21530
                     /locus_tag="J8C37_001053"
     CDS             20565..21530
                     /locus_tag="J8C37_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAZ5304510.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21629..22801)
                     /locus_tag="J8C37_001054"
     CDS             complement(21629..22801)
                     /locus_tag="J8C37_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304511.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22788..>22913)
                     /locus_tag="J8C37_001055"
     CDS             complement(22788..>22913)
                     /locus_tag="J8C37_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304512.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         7163 a         4597 c         4114 g         7039 t
ORIGIN      
        1 aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt
       61 actaagagtt ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc
      121 atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt
      181 ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta
      241 gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca
      301 gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat
      361 caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc
      421 caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata
      481 cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca
      541 agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag
      601 aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttctatcta
      661 ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat
      721 tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt
      781 tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg
      841 gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga
      901 acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc
      961 cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt
     1021 tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc
     1081 aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg
     1141 ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg
     1201 aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt
     1261 tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg
     1321 aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat
     1381 tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg
     1441 gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa
     1501 acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac
     1561 aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg
     1621 atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg
     1681 tgattaacgg caaacaggaa tatgaagtat taggaacgga tatctcatgt aacttaggct
     1741 cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga
     1801 ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa
     1861 acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg
     1921 caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca
     1981 tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag
     2041 ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa
     2101 ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc
     2161 caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc
     2221 aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa
     2281 gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt
     2341 attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata
     2401 tggacatgcg taaagtaatc gatgtttatg caatggcaca acaacacgtc gatcaaggca
     2461 tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa
     2521 caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa
     2581 tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc
     2641 aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa
     2701 aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat
     2761 atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc
     2821 ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta
     2881 ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat
     2941 cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc
     3001 atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa
     3061 gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag
     3121 cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt
     3181 ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac
     3241 aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga
     3301 acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa
     3361 acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat
     3421 acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat
     3481 aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat
     3541 gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc
     3601 tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat
     3661 ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag
     3721 tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt
     3781 cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat
     3841 agattttccg gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca
     3901 gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag
     3961 gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt
     4021 ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga
     4081 caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc
     4141 cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc
     4201 tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt
     4261 cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag
     4321 acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg
     4381 taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata
     4441 aactgactaa tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt
     4501 cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg
     4561 tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag
     4621 gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa
     4681 aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat
     4741 aatagataaa accagcaaca agcaatggta aaaaaactgc cgaaagcatt ttcaatattg
     4801 gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct
     4861 ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag
     4921 gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg
     4981 cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa
     5041 cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt
     5101 caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta
     5161 tttggctggg cttattactt gatgtaggac atctatttta caacctcaga tcatttattg
     5221 cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac
     5281 atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta
     5341 gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa
     5401 ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt
     5461 agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa
     5521 actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc
     5581 ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc
     5641 cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg
     5701 aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc
     5761 aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc
     5821 tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat
     5881 ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc
     5941 cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac
     6001 gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa
     6061 tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg
     6121 tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa
     6181 attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca
     6241 atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag
     6301 ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc
     6361 ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat
     6421 tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt
     6481 cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg
     6541 catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg
     6601 gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt
     6661 agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt
     6721 cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg
     6781 ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt
     6841 tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat
     6901 attttggtat agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc
     6961 aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga
     7021 tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct
     7081 ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac
     7141 attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct
     7201 ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc
     7261 atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa
     7321 ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa
     7381 actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat
     7441 agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac
     7501 caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc
     7561 gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg
     7621 attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat
     7681 cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg
     7741 tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac
     7801 atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag
     7861 gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag
     7921 accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga
     7981 caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc
     8041 tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc
     8101 atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat
     8161 attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt
     8221 gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt
     8281 tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa
     8341 tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat
     8401 caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt
     8461 gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccaac gaaccatatc
     8521 gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc
     8581 atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa
     8641 ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac
     8701 aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa
     8761 cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct
     8821 tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa
     8881 ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc
     8941 agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt
     9001 acataaacat aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccttgtactc
     9061 gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa
     9121 gcaaaatggc caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa
     9181 caagtcccat atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag
     9241 gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc
     9301 aattgtaggc gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc
     9361 caaacatgaa aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat
     9421 gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac
     9481 gcggcatttt ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat
     9541 agatcatccg ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa
     9601 tccccacgag taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg
     9661 ctagtaccgc accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa
     9721 aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg
     9781 cagataacgc tgccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa
     9841 ctaaaaacag taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg
     9901 cacttgtgct aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca
     9961 tattggcaac aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag
    10021 gttttttttc attactcttc attgatttcc cccatttcct atcttagttt acaacttatg
    10081 gaaaagattg gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt
    10141 ttccttgttt tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa
    10201 cacttgcaac caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac
    10261 acgcatacaa cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta
    10321 gaggcaattt catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca
    10381 tatagcctaa cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat
    10441 gattcgtttc ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga
    10501 tgttaccaag agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca
    10561 aaccagcaat gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg
    10621 tcaatacgat caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct
    10681 ctgccttact cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca
    10741 accgatagcc cactaccagt atcagaatga ttcccaacga gatcaatatc aatcggatta
    10801 ttttcttttt catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat
    10861 aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt
    10921 atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt
    10981 ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt
    11041 tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag
    11101 ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag
    11161 cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta
    11221 ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca
    11281 atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat
    11341 taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg
    11401 actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt
    11461 gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt
    11521 tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct
    11581 tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca
    11641 gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca
    11701 tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg
    11761 actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt
    11821 tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt
    11881 ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt attattcatc
    11941 acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa ctcctttcgc
    12001 tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt cacaataaaa
    12061 aactctatgc caccgcagac atagagggac tattgaatat attcaataaa cgtcctcggt
    12121 aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat taacctcaac
    12181 tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat caatcaacat
    12241 tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa atgttgttgt
    12301 gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt ttagcaatac
    12361 atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa cttttccgat
    12421 tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag ctttcacttc
    12481 gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg gcattaagcc
    12541 ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag gtgttgcaac
    12601 aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt cgtcacctac
    12661 gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa aaactaaaac
    12721 agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt gttggtctgc
    12781 ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa attttgcaat
    12841 gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac ttgaatcaac
    12901 tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc ctccttgatt
    12961 gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga gccaactgag
    13021 aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt cgtgcagttc
    13081 cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc atttttaatt
    13141 cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg tttggttcgc
    13201 ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc ggtgtttttg
    13261 taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg atcaaacctg
    13321 cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg atacccgctt
    13381 gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga atttgcaatt
    13441 taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg tgatgtggtt
    13501 aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat ccagacaaat
    13561 aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt ttcaagattt
    13621 ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa aaaaacaaca
    13681 atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt caacagatga
    13741 aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat aacaaaatga
    13801 ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg gacctgtgat
    13861 gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa tcatacgtag
    13921 tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat ttgcaaaaac
    13981 ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga tggaacccga
    14041 tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg aagtcttgtt
    14101 tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc taaaaaaagg
    14161 aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc agatttctga
    14221 attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg ttgtccacca
    14281 agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca atacgaatat
    14341 cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga taattgaagt
    14401 agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa atatcgacag
    14461 cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag aattagtaga
    14521 tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa tggaaatgag
    14581 tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag taatggaaca
    14641 atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg gtggtgatgc
    14701 aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa caatcttaaa
    14761 agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt tgatttgtgc
    14821 tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg tccgagatcg
    14881 gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg ctttcgggtt
    14941 agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag cagaagttgg
    15001 ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga ctcctcatca
    15061 agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt gcgatcccgt
    15121 tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt ctcaagcttt
    15181 tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg atgaagtcgt
    15241 tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa cagcagaagg
    15301 tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa ataagagggc
    15361 gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa caaaaaacga
    15421 agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc gtatttttta
    15481 cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct ttgttcaggt
    15541 gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa atcactggtc
    15601 ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc tctcctcgac
    15661 ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca atttttggca
    15721 atacttcaaa ggacgcagga cctccatcta gttgacgtcc accatgattg gatacccaga
    15781 taccatctac ccctgcagag atagcgatta ctgcatcttc tggcgattgt attcctttta
    15841 cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt gtcaagcctt
    15901 gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca cttgcacttt
    15961 gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga ttgatgacgt
    16021 cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct ttagcccctg
    16081 cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc atatataatt
    16141 ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc gtatttgcat
    16201 aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta tcagcttccc
    16261 ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt gtatctaatt
    16321 caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat atccgtggca
    16381 tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca tcttcagaac
    16441 caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga ccttctagtt
    16501 aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa gtcatagtag
    16561 atccttcttt catcttcttg attacatata aatcatacta aacttacaaa aagtaagcaa
    16621 acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact tacaagctga
    16681 tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt cactactttt
    16741 tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc actgtttaac
    16801 ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa gaaaaatcga
    16861 gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt agaaacgaca
    16921 gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac aaaatcatta
    16981 aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc ctgtccatac
    17041 gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat gttgacttta
    17101 tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc tgcttgattc
    17161 cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc tgcttttcca
    17221 gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac caattctttt
    17281 actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt ttctgtcccg
    17341 ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt actggattct
    17401 gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg gcaaccactt
    17461 aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca taaatttttg
    17521 attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact tgaaaggcca
    17581 atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt gatcgaaatc
    17641 aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt tttctttttc
    17701 tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga tgattttcac
    17761 gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga tagaacgcaa
    17821 gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc cgtgtgaacc
    17881 aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt ctgtcatgat
    17941 catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt ctttcccatt
    18001 tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt cggccatttt
    18061 catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc ctgaatatgt
    18121 atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat ttcattgtaa
    18181 aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc actaaaagta
    18241 cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg gctgtatcca
    18301 ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac gtatctttac
    18361 ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg ctttttagaa
    18421 atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta atgtatattg
    18481 attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc cttcactgac
    18541 tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt ttttctttgc
    18601 catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt atcacgtaac
    18661 tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat tatgatatga
    18721 ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta ttaaaaacac
    18781 ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta gaagaagcat
    18841 taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct tcaggtaata
    18901 ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat ggcggtgata
    18961 tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac ctggatactt
    19021 ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc cttgacagtg
    19081 aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc tctcaatctt
    19141 ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag caaggagatt
    19201 tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt attactcttg
    19261 atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct aacactgaag
    19321 cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa gtggtagaaa
    19381 ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa cagctaatct
    19441 gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg cgcgatctaa
    19501 ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg caagcgtgcc
    19561 ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa tcagtcccat
    19621 gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt tcactttgtt
    19681 ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa aaaccacatc
    19741 catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa ctccccaaag
    19801 caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa actggatcgg
    19861 ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca tgatgatgcc
    19921 atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt tctctcctct
    19981 tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg aaacaaacaa
    20041 taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa aaaagcaaaa
    20101 gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt aagtgtaagg
    20161 ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag aattgttgtg
    20221 aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta gagaacggtc
    20281 tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc cttacgtaag
    20341 taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac ctttttcatt
    20401 ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt tttgaaaaaa
    20461 caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa ccagcaaaca
    20521 ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa ttccattatc
    20581 atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca gaaagaattt
    20641 taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa tacaaaggac
    20701 acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg atagaagaag
    20761 aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac ggcaccttac
    20821 atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct tatattccag
    20881 caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc aaaaaaggac
    20941 ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct tatgaagaaa
    21001 aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta gatgcagcta
    21061 tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac aacttaggat
    21121 cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga tttccaattc
    21181 tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt acagcaacga
    21241 agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg tctaaaccag
    21301 tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg ctcattcttt
    21361 tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca acaaacagat
    21421 tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa atggaaaaac
    21481 gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa taaaagaggt
    21541 tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt gacacgactt
    21601 ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt tgccatcgat
    21661 agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca aaagcaacta
    21721 agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg agtgtttcac
    21781 tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct attttgctaa
    21841 ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa ctgcctgtga
    21901 taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa gtgatacaag
    21961 ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga taccataccg
    22021 ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag ccactgaaca
    22081 acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt cgtttggtaa
    22141 tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga ttgatgacgc
    22201 tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag gttgtgcccg
    22261 tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct ccagtaaccg
    22321 agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct tcttgaaaca
    22381 gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta gaaaaggcat
    22441 ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt tcggtaaagt
    22501 ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca tcgatttctt
    22561 tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat ttacgaaggc
    22621 catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct tgcagagagg
    22681 tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg ataattacac
    22741 ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta taaagcgtca
    22801 taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat aaatccgatc
    22861 aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag ttt
//
LOCUS       DADOOS010000032        22875 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.032, whole genome shotgun sequence.
ACCESSION   DADOOS010000032 DADOOS010000000
VERSION     DADOOS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.032"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            9..80
                     /locus_tag="J8C37_001056"
     tRNA            9..80
                     /locus_tag="J8C37_001056"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Glu,seq:ttc)
     gene            346..1539
                     /locus_tag="J8C37_001057"
     CDS             346..1539
                     /locus_tag="J8C37_001057"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAZ5304513.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1669..3141
                     /locus_tag="J8C37_001058"
     CDS             1669..3141
                     /locus_tag="J8C37_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5304514.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3224..4012
                     /locus_tag="J8C37_001059"
     CDS             3224..4012
                     /locus_tag="J8C37_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAZ5304515.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4079..4747)
                     /locus_tag="J8C37_001060"
     CDS             complement(4079..4747)
                     /locus_tag="J8C37_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAZ5304516.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4862..6574
                     /locus_tag="J8C37_001061"
     CDS             4862..6574
                     /locus_tag="J8C37_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304517.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6577..8346
                     /locus_tag="J8C37_001062"
     CDS             6577..8346
                     /locus_tag="J8C37_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304518.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8551..9117)
                     /locus_tag="J8C37_001063"
     CDS             complement(8551..9117)
                     /locus_tag="J8C37_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ5304519.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9117..10073)
                     /locus_tag="J8C37_001064"
     CDS             complement(9117..10073)
                     /locus_tag="J8C37_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAZ5304520.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10073..10729)
                     /locus_tag="J8C37_001065"
     CDS             complement(10073..10729)
                     /locus_tag="J8C37_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAZ5304521.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11099..13117
                     /locus_tag="J8C37_001066"
     CDS             11099..13117
                     /locus_tag="J8C37_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5304522.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13544..15958
                     /locus_tag="J8C37_001067"
     CDS             13544..15958
                     /locus_tag="J8C37_001067"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAZ5304523.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16306..17859
                     /locus_tag="J8C37_001068"
     CDS             16306..17859
                     /locus_tag="J8C37_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAZ5304524.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18240..20024)
                     /locus_tag="J8C37_001069"
     CDS             complement(18240..20024)
                     /locus_tag="J8C37_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304525.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20039..21760)
                     /locus_tag="J8C37_001070"
     CDS             complement(20039..21760)
                     /locus_tag="J8C37_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304526.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21860..22621
                     /locus_tag="J8C37_001071"
     CDS             21860..22621
                     /locus_tag="J8C37_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ5304527.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7256 a         4103 c         4736 g         6780 t
ORIGIN      
        1 attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg
       61 ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt tttatctgaa
      121 agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct aagctttttt
      181 ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa aaaatacgag
      241 cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag gatccgcagg
      301 acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt agaaagacac
      361 ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga ccaaatcagt
      421 gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc gtgtgaaacg
      481 tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc atatgtagat
      541 attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc aaaattcgga
      601 tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc tgatattgct
      661 caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt attagatgag
      721 attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac accagaattg
      781 atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga tttgcgtaaa
      841 tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt tgaatatgat
      901 gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca tgatgatgca
      961 gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga agttattcca
     1021 gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg atttgtcatt
     1081 ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga tacgtatggc
     1141 ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa agttgaccgt
     1201 tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg gcttgctcgc
     1261 aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc gatctcaatc
     1321 aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt gagagaaaac
     1381 ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc gatttacaaa
     1441 caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg ggaacatacg
     1501 gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag ctggaacgat
     1561 cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa aaagatagta
     1621 attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat gaaaaaagaa
     1681 acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac ggctattgaa
     1741 ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg aatggaaatc
     1801 atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc gatttacgga
     1861 aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt attgtttatt
     1921 atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc tgctcgtgcg
     1981 attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat cggcgatctt
     2041 tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg ggggattgcc
     2101 agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg gcactggatc
     2161 ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct atatttaaat
     2221 gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt tagtttgatg
     2281 gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg aatgagtccg
     2341 cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt ttttgccgaa
     2401 aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact atttgttgtg
     2461 gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt ttatatccct
     2521 atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt attagcacca
     2581 ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac tcaaatgaaa
     2641 actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc ttggttgtat
     2701 cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt catcggtata
     2761 ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt atctcgtgac
     2821 cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca aacagtcatg
     2881 gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc agcagcaaat
     2941 ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc taaatatatt
     3001 gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca ttccgtttat
     3061 ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt tcatgaaaaa
     3121 agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat cattcaaatt
     3181 taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat ttattcaagc
     3241 aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac taccgcaaac
     3301 aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac ctgttttata
     3361 tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt cgattgaacg
     3421 ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg gctggtacac
     3481 agatacagtt tatgacatga agtattggac attcattgca gaagagctac ctgagatttg
     3541 tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg ctggattatc
     3601 gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt tcgcagcagt
     3661 aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt tgatggttcg
     3721 taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag gttcaaaaaa
     3781 tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc caagatttta
     3841 tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg tatctgctat
     3901 gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata actggatctt
     3961 ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat aaaaaaagag
     4021 tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag gagctttttt
     4081 actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag gtttgtgcac
     4141 tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt tctttaaatc
     4201 cctctaattg attttcaact acataattac gcataaatag aactgtttct ttcaaaaatt
     4261 catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc attaactgtc
     4321 caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact gaaaatcctt
     4381 cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga aaagcgggca
     4441 cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca tagtaatttt
     4501 ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc gctaattctt
     4561 cttccattgt ttcagccatt atttgcaaga gctgttgttt gtttttgaaa taccaatata
     4621 aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc tgcgaaagag
     4681 cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga gaaagcttta
     4741 tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa attgttagtc
     4801 ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt gtaatcaacg
     4861 aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta tttccttgct
     4921 gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac agcaagtatt
     4981 ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt acctgatcag
     5041 tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg caaaagtagc
     5101 tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga cattttcatt
     5161 tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa atgatatcac
     5221 gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc cattcttatt
     5281 tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga tcatcgtagc
     5341 gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg gcaagcactt
     5401 catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa acttactagg
     5461 gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat tcagcaaagt
     5521 aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg tgatgatccc
     5581 gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg tgagcgatct
     5641 tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact acttgatgca
     5701 gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa gagcagccgt
     5761 atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt atttagacgt
     5821 tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc gttatcctgg
     5881 tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag aaatggttgg
     5941 tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc ctcgtctatt
     6001 tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag taaatgaaaa
     6061 gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct caggaacgat
     6121 tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg aacatgcttc
     6181 ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg aagcaccaat
     6241 agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca ttactcgtgg
     6301 cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac tggatgctcg
     6361 ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa cgacgattgt
     6421 catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat tggatgaagg
     6481 cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg tctatcagga
     6541 aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat aaaagcaagt
     6601 aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat ctttatcaca
     6661 gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga agcttttcaa
     6721 gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag caaatttgtt
     6781 tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa ctttatctat
     6841 agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat cggtctattc
     6901 aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg tgaaatttta
     6961 agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc tttgcttcaa
     7021 gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg ccaaaatgta
     7081 gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc tgtcttgatt
     7141 atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa actaaatggt
     7201 tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt acaagaagaa
     7261 acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt caaaggacaa
     7321 gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa tacagcgata
     7381 gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc ggtggcatta
     7441 ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact gatgcagatt
     7501 tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt gaatgaaata
     7561 tttgatgaaa aagatgaagt caaaccagaa aactgtaaag catttgatgg tgttgaaaaa
     7621 ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt cttgaaagat
     7681 gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac aggatcgggg
     7741 aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg ttcagtagtc
     7801 atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca tgtaggaatc
     7861 gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac attcggcaaa
     7921 ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat tcatgacttc
     7981 atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa cttattcagt
     8041 acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc atctttattg
     8101 attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat ccaaaaagca
     8161 atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct gaaaacaatc
     8221 ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga aggcagtcat
     8281 cacgagttat tagcagaaaa tggattttat gcggaacttt atcataatca atttgtattt
     8341 gaataaagag gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa
     8401 cagcttctca tgtttttgat ttggttggac ttattaccgc agatataagc ttttgagcac
     8461 cgtgaataaa gaggttatga aatcaacgct ctaacctgag tattagagaa aaaaacgcaa
     8521 aacatatcat ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat
     8581 aggcggctgg ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg
     8641 cagtttggaa caaaaagaat caaccatatc cagttctttt tctccacctt catgaccata
     8701 gtagacgaca aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc
     8761 tgtgcgtgta gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag
     8821 attgaaaatc cctgctttga gattttgttg ttcagctatc acttctccta acgtttcgtg
     8881 gccttgtaga aacaatgaga cacgttcttc taactgacgc tgttctagcc tttcacgagt
     8941 agcatcgatt gcttgttgct ggatatcaaa actatagaca tgaccagttt ttccgatgtg
     9001 ttctgctaga aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatcccc
     9061 tggttgcaag atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag
     9121 acgctcacct ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct
     9181 tcatcaattg cattcaagac ttcccatttt ttcaagctcc acattggtcc aatcaaggaa
     9241 tcccacggcg catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc
     9301 tggtcgcaaa tgacattgac atagtcagga cggctcatta attgaaggcg accttcatga
     9361 tagtcccgca acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg
     9421 atatctgaat ctaaaatggt gcgtctgaca ttttctttca tcatttctaa actttcgcct
     9481 ggtaatccgt tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg
     9541 ccatctaaat aagtttgata atcatgtgct cggttgatca gttttgaagt ttcttcaaaa
     9601 gttgtttgca atccaagttc tacccataaa tacatccgct cgttcagttc agcaaggtaa
     9661 tcaactactt catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct
     9721 ggtaaattga ctacttgctc aaatcgttct ttgattattt caactggcgc atgggtgttt
     9781 gtaaaatttt ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg
     9841 tcgatttctt tctggaattg gattggaaga gggtcttcag gtgcaacgat catgtctccc
     9901 gaaccagata cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcgga
     9961 cagtcaaatc ctccatcgat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa
    10021 gcgtaattcc aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt
    10081 ccttctttct gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca
    10141 ggcacagcca agaaagactg agtaagtaac cgccgatgat atcagtagga aaatgaacgc
    10201 ccagataaat gcggcttatc ccgacactta ctataataaa catcatcagc aattgaagca
    10261 atgtgcgtaa ggttcttttt tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa
    10321 gaatcatact gcctgctgca tgtccactag ggaaactgta acttgtttca actactaagt
    10381 gcgccaatga aggacgctct cgcatgaaaa atagtttgat caccgggttg atgattccag
    10441 caattacaat aaatccagaa acgaaccaaa atgtttctgc cctttttttc ttataccaca
    10501 tccataaagc cgcacaagca aataaaatga tgacggtaag aggattaccg aatttagtca
    10561 taaagagttg gtagctgttc agatcaggat aagaagctct gacaatgcct gtaaaccact
    10621 gatcgaaacc agaaagccac gacagatgga actttacgat ccagcccaaa atgaaaaaca
    10681 ggacaagaaa gaagctacca gccacctgaa atgatgtttt atatttcata caattcctcc
    10741 tttgaagcaa tgacttatta tagcattttt ggtccagcga aaaaaacggt aaagagataa
    10801 aaccaatgta tgattcctct ttgaataacc aatattctta taaaaaaaat aactgtaatc
    10861 ggaaagacat tttttggtaa tgcaaatgaa atgttaggga aataaaatca gtgtttttcg
    10921 aaatcctttt aaacaagaac gtagacgtag gaagaacttg aaaatgaaca gaagttgttc
    10981 attacggtaa attacaatat gattacaaaa aacaacaaag ctcttaaata acttttaata
    11041 aatattaaga gaagttataa ttattaataa tagataaatt gaagttggag gatagaagat
    11101 ggaaaacatt gcaagaaaag aacggagacg tttagaacag accaaacgtt ttcgaaaagc
    11161 aaaacgaggg gcagcaatcg tgggaactgc gatggtaggc tgttctgtcg ctgccccatt
    11221 agtacagccg gttcctgttc aagcggatga aacacctgtt cagttcggcg caagaatcaa
    11281 tacagcggca tttatcacag aaattgctac ttatgctcaa ccaatcgctc aagccaacga
    11341 tctatatgca tcagtgatga tcgcacaagc agttgtagaa agcggttggg gcagtagtgc
    11401 gttatctcaa gcaccttatt ataatctttt tgggataaaa ggcagctatc aaggacaaac
    11461 cgtctacatg gatacgcttg aatttttaaa taatcaatgg gtaaataaaa aggagccttt
    11521 ccgacaatat ccttcttttg ctgaatcttt caatgacaat gcctatgtac ttagaaatac
    11581 ttcatttggc aacggttatt actatgccgg tacttggaag agcaacacga aatcctatac
    11641 agatgcgaca gcttgtttga cagggcgtta tgcgactgat cctggttacg caggcaaatt
    11701 aaataatatc atcgcaactt acggattgac ccaatatgat acaccagcta gcggaaacgc
    11761 aggcggtgga gcgactacag gtggcaataa tactggaagt gcaggtagta ctggcggttc
    11821 aagcaattct ggtaatacaa actcaggttc agcaacttcg accacttata ctgtccaatc
    11881 tggtgattct gtttggggaa tctcaaataa attcggtatc acaatggatc aattgatcca
    11941 atggaacaac atccaaaaca atttcattta tccaggacaa aaactgacaa tcaaaggtag
    12001 ccaggaaaat ggctcttcga cgaataattc cggtaacaat accaattctt caggaaatgc
    12061 tggtacatca aacggaggac aaacaactgg tgcgaaatac actgttcaat ctggtgattc
    12121 tgtttggaag atctcaaatg atcacggaat tacgatggct caattgattg aatggaacaa
    12181 tatcaagaac aatttcgttt atccaggaca acagctaatc gtatcaaatg gcggttcttc
    12241 taatgctgga gcaaatactg gctcaaatac tgggaataca agtggctcta gttcgtccag
    12301 ctcaagttct tctaatacag cttcaggaac gaaatataca gttaaagccg gagactctgt
    12361 ttggagcgtc tcaaataaat acggaattac gatggctcag ctaatccaat ggaacaatat
    12421 ccaaaataac tttatctacc caggacaaca actgactgtt tcaaacagtg gatcaacaaa
    12481 cagcacgtca ggtaatacag gttcgtcaac caatacaaat aacagcagtt cagttaatac
    12541 tggcagcagt tcgtctcaag gcacttatac cgtgaaagca ggtgaatctg tttggagcgt
    12601 ggcaaataaa aacggcatca cgatggatca gttaatcgaa tggaataata tcaaaaacaa
    12661 cttcatttat ccaggacagc aattgatcgt taaaggtgga acttcggcaa atacaaatac
    12721 aggttcaact acttctgcag cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac
    12781 atcatccact ggtaatacga tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc
    12841 gaacaaacat catatcacga tggatcagtt aatcgaatgg aataatatca aaaataattt
    12901 catctatcca ggacagcaag tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca
    12961 atcagttact ggttcgaaaa cctatactgt gaaatctgga gaatctgttt ggagtgttgc
    13021 tgacagtcat ggaattacaa tggctcaact aatcgaatgg aacaatatta agaacaactt
    13081 tatttatcca ggtcaaacgt tgattgtagc taaataaaat agctgttcga agaaataacg
    13141 attgaattcc acaaaaatat ttgttactat aaggctactt aaatatgaca acacttgaaa
    13201 aggaagtagt aaaaagactg aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga
    13261 tgcttttgaa ctcgcctttg agttgccctt gctgaatacc cgagtaagca agcggcggtg
    13321 acggccgtta caacgttcga ggccttttca ctaaggtaaa attaggtggt accgcgtaga
    13381 ttacgtccta taaaaagagc aatcttttta taggattttt ttatttcttt tgtgaagttt
    13441 gagaaaacaa agcaaaagga aataaaaata aacagattct gccggttttt tcgttatatt
    13501 tataatagaa acaagtgttt tcaatagaac aggaggacag caaatgagtt acaatcataa
    13561 agaaattgag aaaaaatggc aaaaatactg ggcaaaaacg aatactttca acacccatga
    13621 tgatcctgaa aagccaaaat tttacgcact ggatatgttt ccctatccat ccggtcaagg
    13681 gctacatgta gggcatcctg aaggttatac ggcaacggat atcctttcac gtgtgaaaag
    13741 aagtcaagga tacaacgtgc tgcatccaat ggggtgggat gcattcggtc tgcctgctga
    13801 acaatatgcg ctagatacag gaaacgatcc agctgagttt acccaaaaaa atatcgaaac
    13861 attccgacgc caaatcaatt ctttaggatt tagttatgac tggaatcgcg aagtgaatac
    13921 aactgatccg gagtattata aatggacaca atggatcttt acgaagcttt atgaaaaagg
    13981 tctagcttat gaagcagaag tagcggtaaa ctgggtacct gaattaggaa cagttatttc
    14041 aaatgaagaa gtgatcgatg ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc
    14101 aatgcgccaa tggatgctga aaatcactgc ttatgctgat cgcttattgg atgatctaga
    14161 attagttgat tggccagaaa gcattaaaga aatgcaaaga aattggatcg gtcgttcagt
    14221 aggagcaaat attgaattta aagtggctgg tacggataaa tcctataccg tatttacgac
    14281 acgaccagat actctattcg gtgcaactta ctcggttctt gcacctgaac tagatcttgt
    14341 tagagaaatt acgacccctg aacaaaaagc tgctgtagaa gcttatatcg aagagacagc
    14401 taaaaaatca gatctaaaga gaacggatct tgcaaaagaa aaaacaggag tatttactgg
    14461 tgcttatgca atcaatccag ttaatggcaa ggaaatccca atctggatcg ccgattatgt
    14521 attatcttca tacggcacag gagccatcat ggctgttcca gcacatgatg aacgtgactt
    14581 tgaattcgct caaactttcg gactagaaat tcttccagtt atcgaaggtg gagatgttga
    14641 aaaagctgct tacacagaag atggtacgca catcaactct gaatttctag acggcatgaa
    14701 caaacaagaa gcaatcgata aaatgaatga atggctagaa gaaaatggcg tagggaaaaa
    14761 agaagtcagc tatcgcttac gtgactggct attctcccgt caacgttact ggggtgaacc
    14821 aatcccaatc atccattggg aggacggaac agtcacagct gtcccagaag aagagctacc
    14881 attacggttg cctaagacaa aaaacatcaa gccaagcggt actggagaat ctccattagc
    14941 aaatatcgag gagtgggtca atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga
    15001 aacgaataca atgccacaat gggcaggaag ctcatggtat tacctgcgtt atatcgaccc
    15061 tcataacaaa aaagaactag cagattatga aaagttgaag cgttggctac cagttgacat
    15121 ctacattggt ggagcagaac atgcggtact tcatttacta tatgcacgat tctggcataa
    15181 attcttatac gatatcggtg ttgttcctac aaaagaacca ttccaaaaac tatacaatca
    15241 aggaatgatc ttaggcgaaa acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa
    15301 tcctgatgat gtggtcgaaa cttacggtgc agatacactg cgcatgtacg aaatgttcat
    15361 ggggccatta gatgcttcga ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt
    15421 cttggatcgt gtatggcgtt tgatcgttga tgagaacaat aaaatgcgtg accgtatcac
    15481 tacattgaat gatggcaagc ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga
    15541 agactatgaa aatcttcatt tcaatacagc aatctcccaa ttgatggtct ttatcaatga
    15601 agcctataaa gtggacgctc tgccttatga atatatcgaa ggatttgttc aactactcgc
    15661 accgattgct cctcacatgg gtgaagaact atgggcaatc cttggaaatg atggtggtat
    15721 ttcttacgca ccatggccaa catacgatga agcagcactt gttgaagacg aagtagaagt
    15781 tgttttccaa gtaaacggta aagttcgtgc gaaaagcaat gttccccgag acttaggaaa
    15841 agatgagcta gaaaaagcag cattagccaa tgaaatagtg caagaatata ttgaaggcaa
    15901 aacagtcaga aaagttattg cggtaccaaa caagttagtc aatatcgttg ctaattaatt
    15961 ggctctttgt caataaggac tgatgagttg tgcaaaatca aatctgggcc agaatgaact
    16021 tcattctgtc tcagattttt tgttatttca aattgattaa ttgattgatc aaaaaggttt
    16081 tgattttcat gttctcaaac acgttcaatg tctttttctt ctgaatggtc ttgtaagaga
    16141 tctctgtaat catcaaattt ttgtctatta atcttagtat tttttgatgt ataaactacc
    16201 tgaaattgag agtacatacc aaattcataa attttttatt agtgggacta gtgattgtat
    16261 ggattttgac gcataatgaa tgaagtatga gaaaaggaga gacaaatgga tcaagaacta
    16321 tttattatcg aagtattatc agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg
    16381 aaaagaataa tgttcaagct tttaagctta gtatttttct ctggtttgct ttttttcgga
    16441 gctgtatcgt tcctatatcg ccatatgagt gcagtagtcc gttcagctga agggatgcaa
    16501 aaattaaata atttgtatca cttttcgtgg ggagttggcg gaataacaat tgttgtgctt
    16561 attgtttttc ttctacttat ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa
    16621 tggcttagtc aaagagcaca acagttttta cataataaac caacttctca agatgatgac
    16681 tatctttatt ttgagaagac ttcgacgagt caagcttttc ggatacaaaa aagtgattgc
    16741 cagttattgc tgagatcact tgatgattta cctatctact taggcgaaag caaatcaaaa
    16801 ttcggaatga acacattcca tattttttta ctagaaaacg aacccagatg ggaaaaaagc
    16861 cagtcttcgc ctggtggata cctgacaact aagatgagta cattagtcac tggttttttc
    16921 ctcattcttt tagctggaac aatctattat gggagataca ctcagccaaa aatgtcttct
    16981 catataaatg ctgcttctca catgacaatg aactcaacta acgatacgca tatacaaaag
    17041 acaaatgaag ggttgttagc tgatcctaag caaaaggtaa atttgacaac acaaaagata
    17101 aatgatctca aacttgatac agagacgcac tctctctata tgacaacaga tagtggtgct
    17161 aattgggtat ttgtgccgat tgattcgcaa tggttgcgtt ttggcgaata caccctgaca
    17221 acggggctga ttcctgaagg ttattggatg gacaacacat atgatatttc cgccgatttc
    17281 tcatggttta tctattcttc tggggatgat gtacgtctgc tgacttcaag agataatggt
    17341 aaaacatggc aaacgtctat tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg
    17401 gtattttatg gcaacgatgg tctatttatc tgtacatcag ccagtgggat gtctgcagaa
    17461 actttgtaca tttacactac tatggatggc ggaatcactt ggataaaaag tggaagtaca
    17521 atgatcaatc agccagtaca aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg
    17581 acacgtgaaa gtttgtttta tacaaacaac ggaggacgct cattcaaaga atcagttctg
    17641 tcgattccgg aaaactatca gacagggggg atagatcttt ttcaatcacc aaatgaagtt
    17701 actcaaataa gcgcgaatac gttagagaca aaattttatt tgttgaaaaa aggaccaatc
    17761 gatgcaggga aaatgtttgc ctgcctcttc cgttcaactg ataacgggga aacatggcaa
    17821 ttcgttcagc aactatcgca agtagaacaa ggagattaaa gaagagagat tgtgagcctg
    17881 acgttcagca ccgagtcaca agaaacaaat tcaaaaaaca gcttctcata tttgttgaat
    17941 agtgtaactc gtgattcgga gttgacaaga gcaacaagaa gcgattttga agtatagctt
    18001 ctcgtatttt acaagatcgt gtcttattgc cgataggttg tctcttgaac accgtttatt
    18061 cgtaaaaagg gattgtgaca agtcttttgt cactacttac cgaacgaaac tgaaggagct
    18121 atccaaaaag tatcattagt attattcttt gtgtttctag tggcaccact agtacattta
    18181 aaaaagctat cgatcatgga ctaatcatac atgatcgata gctttttaga taatgtttgt
    18241 tacgctatct gaaattgact attataaaga ctggcataaa gcgttggtga ttggagtagt
    18301 tcagaatgtg ttcctttttc gacgatatcg ccattcttca ttacaaggat catgtctgca
    18361 ttttcaatcg ttgacaaacg gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct
    18421 tccattgctt gctgaatcaa tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct
    18481 aaaataacga caggaggatt cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt
    18541 ccttgagata acgtgccgtt ttcgctagaa atgatcgtat catatccgtc tggcagtgtt
    18601 cgaataaagt gatcacattg tgcgatttca gccgcttcga taatttgttc tcttgtggca
    18661 tccattttcc cgtaggcgat attatcagct accgttcctt caaacagcca ggtattttgt
    18721 aaaaccatcc caaaagaacg acgtagttct ccacgtgaaa gcttggtaat atcaagaccg
    18781 tcgaattcga tctttccttt attcagttca taaaaacgca tcagcagatt gactaaagtc
    18841 gtttttcctg caccagttgg accaacgatc gctaccgttt gttttggctt tactgaaaaa
    18901 tcgacttgat tcatcaatat tttatctgat gtgtaaccaa actgtacatt tttgaaagct
    18961 atttttcctt tcggttgttc taaaagtctt gcatctggaa ggtctggaat ttcatctgtt
    19021 tcatctaata tctcaaatat cctatcgatt gctgccattg cggattggat cgagttgatc
    19081 acataagaag cggtagaaat tggttcagaa atctgattga tgtactgaag ataagcttgt
    19141 aagaagccga tcgtcacacc acctgataga acaagcatag caccgaatac cgcacttatg
    19201 atgaaggcga gttgattaat gaaacgaatg gccggataga tagcacagtt cataaattga
    19261 gcacgtttaa aagcgttgta ttgcttttta tttacttcgt gtatagcagc ttctgcatga
    19321 gcttgctgat tgaatgtttt gaccactaga ttaccagcta agatttcttc cattcgatta
    19381 tttaaaacac ccagttcata ctgattctgt tcagaaatct gtttgttttt gttcgcaatc
    19441 ttagttgtaa caattgaact gccaacgatc agcaataaga cgagcaaagt aagcttgctt
    19501 tcgatataaa acagcatcag accagcagca gctattgtta cgacagaagt aaaaaattga
    19561 ttgatacctg tcaataacac ttgagaaagt tgattgagac cggtagttgt ccgactaatg
    19621 atgtcaccta cttgatgatt gtcaaaaaac gacatcggta atgttttgaa tttttttgtg
    19681 atttcttttc gcaccctcaa tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga
    19741 ataaatgaag tgatgctgct taagactgaa cagccgatca gcaataagac agggaacaac
    19801 aatgcttgtt tgatatcact gaaagaaagc tgtccaatac ctgtttttct tatttctgat
    19861 aagagatcat cgatcccgat tcccatgatc aagggcatgg ctacgactaa taggttcccg
    19921 aataaactgc atagaaacaa tccgtaaaaa tatttatgtt caggtttgat cattttccaa
    19981 aaacgtatca aagcacgcgt gttcaacgtt tttttctttt ccatcgtatc ccttctttct
    20041 atattccttg tgattttgca aactcctgat aagtttggca attagacagt agttcttcat
    20101 gagtaccttg tccaacgatt tttccttcat ctaaaacaat gatgttatct gcttgttgga
    20161 ttgtactgag gcgttgagca acaatcacga gagttttctc cgacatgtgt ttatgaaggg
    20221 ctgctcttaa tgctgcatct gttttatagt ctaaagcgga gaaactatca tcgaatacat
    20281 agatctctgc tggtttgatc aacgctcgag caatacatat ccgctgtttc tggccccctg
    20341 aataattaga accgccttga gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta
    20401 gttcttctaa ttgagcgatc ttgatcgctt tagacaactc ttctgttgag gcatattcat
    20461 ttcccatctg caagttgctt aaaattgtcc cgctgaataa gaaagccttc tgagggacat
    20521 aactgatgtg ggagcggatc tgttcttgag tcatttgagt aagaggaata ccaccaaggg
    20581 ttacttttcc tatcgtaggg tcactaagtc tcaagagaag tttcgccaaa ctactttttc
    20641 cagatccggt cccgccaacg atcgctgttg tttttccttt aggtattgtg aaatcaatat
    20701 cttctagaac tggatcagag atttcagtat atttaaatgt gacatgttca aaagcagctg
    20761 catttgtctg atcaaaggtt ggttcagcta ctgttttgat cgtctcttcc tcaatttcga
    20821 tctctgcttg cagtatttct tctatccgtc gtaatgaggc aaacgattta gggataatga
    20881 ccaataccat agctgcgatc atcaagtaag caagtgtcag catagagtat tcgactacag
    20941 cagtcacgac accgatttgt aatgctcctt ctgctacaag atttccgcca aaccacaaaa
    21001 tagaagcata aacgattccc atgagcaaaa aagcaattgg agtgacccaa gcaaatattc
    21061 ggttcacttt gatcatttga ttagcatagt cagcaaagct cttattcgtt cgcttttctt
    21121 catatgattg attatcaaag gcacggatca tattaatccc agtaaagaat tctctcaatg
    21181 tgatggtgat ccgatccatt tttggctgga tactcaaaga taaaggtgcc cctcgtttca
    21241 tcagtcgata aacaatccat acataaaaaa caacagagat caatgtaacc catgttagta
    21301 atagagaata acgaaacatc atgagaatgg tgaaaacgca aataaccggt gctggtaaga
    21361 tcatttggca aaataaaaca atcatttgct ggacattatc gacatcattc gtcattcttg
    21421 tcaaaagaga tccagaacta aaacgactga tatccttcat tgacagctgt tggaatttac
    21481 ggaaatattt ctcccgcgtt tggaatccaa acctagcgga agccaatgct gaaaagtaac
    21541 tagaggcaac tgcagcgatt gttcctagaa acgctacaag cagcatttca actccaattt
    21601 tttttataac tgaggcttcg ccacttgcta tacctgtgtc gatcagcttt gctactaatt
    21661 ttggtacacc taatgtaccg gcgatttcta aacagatcat cacaaaggta aggagagcga
    21721 gtcctttgtt tttctgtaag aatgttttta agatagacat attattcctc cttttttcct
    21781 gatacttttc gattctatac cttcaagtaa cttgaaggtc aatagaaaaa agaataaact
    21841 gaaaaagagg tgagaaaaaa tgaaaaaaat gtatacgatt ggccaagtat cggaactatt
    21901 tgatttgcca aaatcaacga tccgctattg ggatgaacaa ggattaatcc gttcaagtag
    21961 acaagaagag aatgattatc gattgtttga tatcgatgat atttttatgc tttatgatat
    22021 cgttttttat agaaaattag atataccgat caaacagatg aagaatttat atggaaaaac
    22081 attgacggag ttatatgaga cacttgatga aacagaaaga agaatccata aagaactggt
    22141 ggtgatgaaa caaaaacaaa aagagataag ggagcaaaaa aaacagttga aattgatgat
    22201 cgatacaaac gaggaagaat ttcctgtaga agagatccct tttgactgta tgatctcaac
    22261 ggagtttgaa gatattgtgg aaatcaaaaa atttctacct aactattcaa gctttgggat
    22321 gatgagtatg ccagcttcca gttcacagac gatgtatgga ttttttatcg accctagtga
    22381 agtacattta tttactcaag acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg
    22441 attcttattg aaatccgaga tgaaccatgc ggaacgtaat aatattttag aaatcagaga
    22501 aagaatgtat gaaaaaggat ggaaaacagg agaagtgatc ggccagtatc tattgacgaa
    22561 tacggatgaa aacaatatca gaactgaata ttatcatgca tggatagaaa tgaaaaaata
    22621 ggaaatatgt gtttaagata actcgaaaat gatggttgtt ttagttataa agaaaaattt
    22681 tctggttcga ttacgggttt aatgtggggg attattagtc gtggatagat agaattttag
    22741 ttttttagag gaaatatgtc tttaaaaatg gaatggttgc aattcttagc gatatatacg
    22801 atgtaattgt cctcttaaaa aagtatggaa aaataattgt attgggtgtt atgtataatt
    22861 catggtgaac tgaga
//
LOCUS       DADOOS010000033        22190 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.033, whole genome shotgun sequence.
ACCESSION   DADOOS010000033 DADOOS010000000
VERSION     DADOOS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.033"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..867
                     /locus_tag="J8C37_001072"
     CDS             <1..867
                     /locus_tag="J8C37_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5304528.1"
                     /translation="NRIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHA
                     FIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVL
                     LTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTL
                     PLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLI
                     KKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRA
                     AEALENAFGFKE"
     gene            1441..2010
                     /locus_tag="J8C37_001073"
     CDS             1441..2010
                     /locus_tag="J8C37_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAZ5304529.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1997..2857
                     /locus_tag="J8C37_001074"
     CDS             1997..2857
                     /locus_tag="J8C37_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ5304530.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3047..3751
                     /locus_tag="J8C37_001075"
     CDS             3047..3751
                     /locus_tag="J8C37_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304531.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3756..5591
                     /gene="walK"
                     /locus_tag="J8C37_001076"
     CDS             3756..5591
                     /gene="walK"
                     /locus_tag="J8C37_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAZ5304532.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5588..6904
                     /locus_tag="J8C37_001077"
     CDS             5588..6904
                     /locus_tag="J8C37_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304533.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6905..7774
                     /locus_tag="J8C37_001078"
     CDS             6905..7774
                     /locus_tag="J8C37_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304534.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7829..8638
                     /locus_tag="J8C37_001079"
     CDS             7829..8638
                     /locus_tag="J8C37_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAZ5304535.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8741..9370)
                     /locus_tag="J8C37_001080"
     CDS             complement(8741..9370)
                     /locus_tag="J8C37_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAZ5304536.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9540..10832
                     /locus_tag="J8C37_001081"
     CDS             9540..10832
                     /locus_tag="J8C37_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5304537.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10908..11570)
                     /locus_tag="J8C37_001082"
     CDS             complement(10908..11570)
                     /locus_tag="J8C37_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAZ5304538.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11795..12079
                     /locus_tag="J8C37_001083"
     CDS             11795..12079
                     /locus_tag="J8C37_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAZ5304539.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12130..13755
                     /gene="groL"
                     /locus_tag="J8C37_001084"
     CDS             12130..13755
                     /gene="groL"
                     /locus_tag="J8C37_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAZ5304540.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13844..14992)
                     /locus_tag="J8C37_001085"
     CDS             complement(13844..14992)
                     /locus_tag="J8C37_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5304541.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15094..15678)
                     /locus_tag="J8C37_001086"
     CDS             complement(15094..15678)
                     /locus_tag="J8C37_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAZ5304542.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15805..16236)
                     /locus_tag="J8C37_001087"
     CDS             complement(15805..16236)
                     /locus_tag="J8C37_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAZ5304543.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16254..16574)
                     /locus_tag="J8C37_001088"
     CDS             complement(16254..16574)
                     /locus_tag="J8C37_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304544.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17312..17569
                     /locus_tag="J8C37_001089"
     CDS             17312..17569
                     /locus_tag="J8C37_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAZ5304545.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17540..17848)
                     /locus_tag="J8C37_001090"
     CDS             complement(17540..17848)
                     /locus_tag="J8C37_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304546.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17926..18672
                     /locus_tag="J8C37_001091"
     CDS             17926..18672
                     /locus_tag="J8C37_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAZ5304547.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18687..18890
                     /locus_tag="J8C37_001092"
     CDS             18687..18890
                     /locus_tag="J8C37_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304548.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            18995..19294
                     /locus_tag="J8C37_001093"
     CDS             18995..19294
                     /locus_tag="J8C37_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304549.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19294..19476
                     /locus_tag="J8C37_001094"
     CDS             19294..19476
                     /locus_tag="J8C37_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304550.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19482..19958)
                     /locus_tag="J8C37_001095"
     CDS             complement(19482..19958)
                     /locus_tag="J8C37_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304551.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            20011..20736
                     /locus_tag="J8C37_001096"
     CDS             20011..20736
                     /locus_tag="J8C37_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAZ5304552.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            20910..21251
                     /locus_tag="J8C37_001097"
     CDS             20910..21251
                     /locus_tag="J8C37_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304553.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21244..21915
                     /locus_tag="J8C37_001098"
     CDS             21244..21915
                     /locus_tag="J8C37_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAZ5304554.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            21921..>22190
                     /locus_tag="J8C37_001099"
     CDS             21921..>22190
                     /locus_tag="J8C37_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304555.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
BASE COUNT         7521 a         3715 c         4456 g         6498 t
ORIGIN      
        1 aatcgaattt acaagttttt ttatcctaaa aaaattaaac taaataggtt aaagccttat
       61 catttacaag agttttatca atatatgtta aatgataact gtacagcaaa tacggttatt
      121 cattatcacg catttatacg caaagctctt caagaggctg ttattacaga attgataagt
      181 acaaatgtag cagataaagt aagaaagcca aaaaagaaac aatttgtttc tcaagtttat
      241 aatcaacaag agatcttgaa attacttgac attttgtcag aagaaaaact ctatttggta
      301 gttcttttaa cagcatttta tggattgcga agaagtgaag ttttaggatt aaaatggtct
      361 gctattgatt ttttaaacaa aaaaatcatg ataaaccatg taatcataga aaatccagag
      421 aatatgtcac agttaataaa gaaagatcaa acaaagaact cttccagcta tcgtacatta
      481 cctttggttg aaacgattga acgagcatta gtaaatcaag ctaaatggca acaagataat
      541 cgtattcttt taggagatga ttatatttta gaagataatg agtacgtatt tactatggaa
      601 gatggacggt tgatgaagcc tcaatatgta acgcatcgtt tgagtaagtt gattaagaaa
      661 aatggattga aaaaaattcg ctttcatgac ttgcgtcatt ctaatgcttc aataatgtta
      721 gatagtggcc agaatatgaa atccattcaa gaatggctgg gacatgcatc gtattcgaca
      781 actgctaatt tatacacgca tttaaccgca ggagtaaaag atcgtgcagc agaagcatta
      841 gaaaacgcat ttgggtttaa agaataaaaa aacaaaaaac aatatctttg gggtatcgct
      901 tcttgttttt agaatttttt tgttggcaat ttgttggcag tatgttggca atgaaaaaat
      961 tcacaaacta aaaacctatt tataaagctt tataaaactt ttttcacaaa tagagcattt
     1021 ggttaagagt cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa
     1081 tagtagcact aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt
     1141 acttccttat aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata
     1201 ccaacaaaca aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct
     1261 ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc
     1321 cttcttgttt tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag
     1381 attgaatgtt tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg
     1441 atggaaaaaa tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg
     1501 cctcactggg aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt
     1561 gatcgttatc tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg
     1621 gtaaacaatt acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa
     1681 gaacacgtag catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa
     1741 atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta
     1801 ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct
     1861 catccgcaaa aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact
     1921 ttatcgtttg caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta
     1981 aaagaggaga aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga
     2041 tgttccagaa gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta
     2101 taaagatcag gtatatacag ataaaattga cattacccct gaagagatct atcaacgttt
     2161 gccagaggaa atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga
     2221 gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag
     2281 cgggacatat aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt
     2341 actagatacg aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg
     2401 gctggaagaa ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag
     2461 aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg
     2521 tttagttgct tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga
     2581 agaagggatt tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac
     2641 ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca
     2701 tggggatgcg aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca
     2761 agcaaaccaa atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg
     2821 attattgggt gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg
     2881 tgaaaatcag tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc
     2941 actttcattt taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac
     3001 ggtataatga gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt
     3061 agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg
     3121 ctatgacgtt tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga
     3181 acctgatttg attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg
     3241 tgaggtacgc aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat
     3301 tgataaagtc ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa
     3361 ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga
     3421 accagaagag gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc
     3481 ttacatggtg acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact
     3541 cttttacttg gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt
     3601 ttggggttat gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg
     3661 tgaaaagatc gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg
     3721 ttactatttg cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc
     3781 gatcagtcaa ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa
     3841 ttattggtgc ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag
     3901 atatgaatca aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag
     3961 ataatgcaga tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga
     4021 ctagcgatat catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg
     4081 atgtcaatga acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca
     4141 cgactaaacg ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac
     4201 cgatcctgtc cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg
     4261 aacaaaaata ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg
     4321 cggcatttat ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg
     4381 gtgaaatgag agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag
     4441 tgcatggaca agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta
     4501 ttgaagaagc tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc
     4561 atatgacaga cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata
     4621 tggctgtttc cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac
     4681 ttttagatat cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg
     4741 tacttgaccg ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt
     4801 ctatgatccg tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga
     4861 cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac
     4921 ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga
     4981 aagacgagga agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga
     5041 ttcgaatgat caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc
     5101 aattagaata tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga
     5161 tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt
     5221 gggtcgaagt agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga
     5281 tcaagtattc accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg
     5341 tgattttaag tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt
     5401 tcgagcggtt ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg
     5461 gattagctat ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca
     5521 aggaaggaaa agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat
     5581 ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt
     5641 aagcatatac ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt
     5701 agatgagaca aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt
     5761 tcttccgctt catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa
     5821 tctcgtgtac cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt
     5881 agtttcagga gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt
     5941 ggcctataat gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga
     6001 tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca
     6061 aggaaataaa aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat
     6121 ttcgttgaat tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga
     6181 aatgacaaca gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact
     6241 gaagaagtat agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt
     6301 ccagaaacca gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg
     6361 gaatgaaaca atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc
     6421 agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg
     6481 cagttcttta gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat
     6541 attcgtggaa ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt
     6601 tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca
     6661 agtgccgata ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca
     6721 gttaaaagct aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg
     6781 gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata
     6841 taaagataac tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa
     6901 ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt
     6961 gtttttattc agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa
     7021 tcagactgaa tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat
     7081 ttcgtctgag aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc
     7141 attagcgaat tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga
     7201 cggtgatgtt ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc
     7261 agataaaatt agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg
     7321 taatgattat gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca
     7381 agcaagccaa gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc
     7441 tttagaaaaa aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga
     7501 tatcgagcag ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt
     7561 gtacatcaat aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc
     7621 ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga
     7681 aacaagcgat aaagtggtcc aaattgagag cgtcaacgct ttcagcaata cgatcgtgac
     7741 aaacaatact gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt
     7801 acaaagtaag aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag
     7861 tatcctagca agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct
     7921 tttggtcgat gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg
     7981 gacaccggac caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg
     8041 cgtgggtgtc ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga
     8101 agcgatggaa ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg
     8161 gaaagtcaga acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc
     8221 ggctccgcaa ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac
     8281 cggatattgc agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga
     8341 aagtaatcat gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg
     8401 tattttagga gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt
     8461 gatcggtaat cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa
     8521 agaacttgct catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca
     8581 tgattttgaa atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac
     8641 agttcaatca ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct
     8701 tctattttta tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga
     8761 tgtgctgctt ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg
     8821 ccatgctgat cgtactgtcg ctagcagcgt ttctttgtaa ataatcatca cttaacaaat
     8881 aagtacctgt acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat
     8941 gtagattttt agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat
     9001 agaagggtgc atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga
     9061 ataacgaaga ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa
     9121 tcaaatggga caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat
     9181 caacgacaac atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta
     9241 taaacggaac atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat
     9301 ctctggacaa tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg
     9361 caattttcat aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt
     9421 cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat
     9481 tgggtatagt taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa
     9541 tgaaagagtg gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac
     9601 ccttaactgc tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc
     9661 tgtttttctt atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag
     9721 gattttctat gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga
     9781 tcgtttttgc cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg
     9841 gcataacagc cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata
     9901 ctgtctggac aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat
     9961 ggggctatgc acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg
    10021 aaaatgtttg gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc
    10081 ttatcggttc agcggttttt caacatttgt tcagaatgac tgttgttcaa ggagtatgga
    10141 catatgtcca gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag
    10201 gtttttttgt tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt
    10261 tggttagtcc cgtcttgcaa gcgaattcat tagcaaatgc agaaatctta gcctcaggac
    10321 aagagttgac ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca
    10381 tgacggttac gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga
    10441 aatcgaagaa atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca
    10501 atgaatcaat tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta
    10561 ttttcgcacc ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc
    10621 ctttatttac agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag
    10681 taggcggagt gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt
    10741 atttcccatt tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa
    10801 atgatggagc agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga
    10861 ttgggaaaat caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct
    10921 ttttggacag ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc
    10981 gatgtccaga tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag
    11041 tagattagca gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct
    11101 ccgacccaaa aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac
    11161 tcttccatga tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc
    11221 atctggatga tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct
    11281 aacatgacag cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata
    11341 acaatctttg gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc
    11401 ttaaaataaa aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg
    11461 atcaattcag acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa
    11521 attagaccat agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc
    11581 cttttaaggg ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat
    11641 aatttcaata agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa
    11701 aagagggaga atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa
    11761 cagattaaaa aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt
    11821 cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc
    11881 tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga
    11941 aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata
    12001 cgccggtaca gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat
    12061 catggcgatt gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt
    12121 gaatcaatca tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc
    12181 ggggtagata aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc
    12241 gtactagaaa aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa
    12301 gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca
    12361 tcaaaaacaa atgatattgc aggagacggt acaacgacag caactgttct gacacaagca
    12421 atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc
    12481 gggatcgaat tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt
    12541 gattcaaaag aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga
    12601 catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa
    12661 tcaaaaggga tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac
    12721 ttatcacaat atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat
    12781 atcttgatca cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa
    12841 atcttgcaac aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg
    12901 ccaactcttg tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct
    12961 ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca
    13021 gtaattactg acgatctagg tttagaattg aaagatgcaa caatcgaaaa cttaggaaat
    13081 gcttctaaag ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa
    13141 gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat
    13201 tttgaccgcg aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt
    13261 aaagttggtg cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca
    13321 ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta
    13381 gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc
    13441 aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat
    13501 gaaggatcag tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct
    13561 gctactggag aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca
    13621 cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc
    13681 gttgcagata aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg
    13741 ggcggtatga tgtagtaatt aagaaagaac cctattttaa cagggttctt tcttcttttt
    13801 ttgttttatt ttaagcaaaa ggggcaaaga aggggcaaaa atatcaaagg ctatttagtt
    13861 tatcaattac ttgttgtttg gctctttttg ttacatggtt ataaatagac agagttgtgt
    13921 ttgcatcaga atgccctaca cgctccataa tggctttaag aggtacgcct aattcagata
    13981 atagtgaaac gtggctatgt ctgaatatat gtgaggatag acttttttct aattccaact
    14041 cttcctctac tttatggagt atagcgttaa atgagtgcag cgcaagcgga gtgccacttg
    14101 tagatataaa tatatattga tcgggatctg tgggtctacc tgcaagaata ttgtcagcta
    14161 tcacactttc aatcaattct tttgcacgat tgggtaattg cacttcacgt tgcgaataag
    14221 tattttttgg agttgttttt atagcattat ccattttcac agatgtgtaa tctaaggtcc
    14281 cattaatgga aattttccca tcctcatagt ccttcatttg caaagctagc aattccccat
    14341 atctcaaacc agttaaatat agaaactcag ctattatgcc gtgtagtttt ctgcgaggat
    14401 tggagtatag ctgtttcagt atttgatcaa tttcttcttt ctccagatat tttttattca
    14461 tagaaagcct tcttttttct tcttccactt ttttaggatg gattttaact gctagcgcag
    14521 ggtttctttg aatgtatttt ctatcgattg catagtttag cataacagat agagttgttt
    14581 ttgtttgttt tgtgtagttc agtgagaggt caccaaacgt atacatatct tcaattatct
    14641 tattaatgag tgtctcatca atgtttctaa cgatcgtatc atcgcttatg tgcttagaaa
    14701 catgtttcat catcattgga accttcaaat agctagtacg tttaacatgc tgcttataat
    14761 attcatacca ttctttatac agctcaccaa aagtgatatc tgatttattg taatcttcga
    14821 gtgcttcttt aattttttta tctagaattt tctgagcttt tttccacgct tgtggtgaat
    14881 tacttgtaag tgttgtagat ttttttcgtg ttttttctgt ataaggatct acatatcttt
    14941 caataaactt gaattggcca tctttggtct gttcaaccca cacttttaac atctcctatc
    15001 atttgctata ataggcataa caaatagacc tatataggtt tgttttctaa aagcacgctc
    15061 ttactctgga cggtggggcg tgttttttat ttattataca gttgctttaa aaatagctgc
    15121 tttttcaaat gatactaaag gagagaagtg gatttctatt tctccctttt ggtttaacgt
    15181 gaaatgagca gttacatcca tttgttttcc tggagcaaca gatcccattg tattttcgtt
    15241 ggcatatgtc tctgattttt tgtcatctgg tccatatact tccacatctg tacctacagg
    15301 gatatctgaa tcgccatcat tttttacggt ataagtaatt tttactactt gtgcaggttg
    15361 gttttcttca aattgatttc tttcatcagt tagttctaca ctatttagtg tatattcagc
    15421 atcaccaact tttacagtgt caccaatctt atagaaggta tcgctttttt cttctttaga
    15481 agatgaagca gaggttgaag atttagttac tttttcgcca ccattatcac ttgctttatt
    15541 actatctgat ccaccattta aagcagaacc aataattata attaaaatta ctgctaatac
    15601 ccaaaaccat acttttttgt aaaaaggttt actaacttta tacatttttc cgtcttgacc
    15661 cataactttt tttgccattt aaatattcct cacttcttgt tataatatat ttgcgatctc
    15721 agaaatgagg tatgagtccg tgtgccagca cgggcttttt taatgttttg ctgaaatcgg
    15781 tttttttaat aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt
    15841 aaaatagcat tggcagacag cataacaagt gtatctgagc ttgttctgaa tagaataata
    15901 ctccataaat ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat
    15961 atttaataaa attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt
    16021 tgtagaagta tcgtacaaaa ctgaatattc aaaatgagaa gcggtgaaat gcgcaagttc
    16081 atgagataaa tgaaaggctt ctgcagtttc gccatataga ttttcattca aaaaaatgat
    16141 tctaggcttc ggataataga agccaggctc tttcatttcc atgtaaacta ctttcaaatt
    16201 gtattcgctc aacatttctt tcaatttcaa atacatacaa cccatcactc caactattca
    16261 ttttcctcta aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct
    16321 tttccatcaa aagaaaaaac agtatcgttt tctgataaat ccacatgttt aggggtttct
    16381 ctttcttctc tacctagaag gtagtctaca gagactccaa agtaatcagc taaccgagct
    16441 aactcaatag aattaggagt ttgtcttttc caacttccta aatatccatt cgaatacccg
    16501 aaattcattt cgagctgtct aatggataat cctttttctt ttgctaactc ctttattatt
    16561 tcgtaagtat tcattgataa atcaaccttt ctgaatgctt acaaaaaaag tttagataaa
    16621 taatgtaaaa atagttgact tatatacatt attaatctat actatgtttt gtaaacaagt
    16681 ttaacaacta aaaagacaac aaaaaatact attgattaat aaatgccaac cgccaagaaa
    16741 gctttttaaa tcaaagttta tatgtcttat ttaactatgc atttagtata gattattaat
    16801 ctaataaagt caacgcagtt ttttaaaaag ttgttaaatt tgtttacgaa tataaaagaa
    16861 aggagagaag aatatggaaa aaacagtctc ggcaaaaatc gaagatttga aattagacat
    16921 actgaaacaa gcaaaagtgg cgatggaaca cgcggtagat aaagaagact ctgccatggt
    16981 tgcagccata gcagagattt tagctcacgt ttagtcatcg tttttacggt cttctggaag
    17041 ctgaccatag ataacagcat aatgaatatc taaataagct tgaacaattt cttttggcga
    17101 aattgattcg cctttagtag atacttgtga ttcgtgattt agccatgcaa caactaaatc
    17161 cgcagcgatt tcaaacggcg ttacactttg tgtcagttgc cataaagaat ttcacaatat
    17221 gtatggtcgt ggtaataata ccaaagagca atttgaggaa tttatggaaa ttaaaaaata
    17281 caaaaaatca tttattaaag gaggctaaac aatgaacaag gaaatgagcc aaacaatcaa
    17341 ggtacaaaaa atgatagatg atctaacaca tggaattgat agccaagccg ataaaatcat
    17401 caaggaattg caaggacaaa aagttaagga tgcaaaaatg cttctcaaga ctatcaactt
    17461 tgaaatgaat ccaacaaaaa gaaagctcgc cgatgtgttg gaagaaaagt tggcttccgc
    17521 tatcaacgag caagaattac tatttgaaac cgatactttt aacagctgat gttttataga
    17581 tgacagtttc atccgaatct atatcatcgc cgagagaaaa gaatgttgct tttgacaaaa
    17641 agccgataag acctacaagc ggtcttgatg ttgcaagagt agatacccca atatcacctt
    17701 gcaaggtatt cacgtaaatt tgagaagaat agaacattcc atctttttct tcgatatttg
    17761 acgcatcatc gtatccaacg accaaagtat cggcaaatat tttgattttt tctccatcaa
    17821 gcatagtaat tacaaattct ggtttcataa attttcacct ccttaacaat tatttcagcc
    17881 tgtcacactg ataaggaaat tataccaaag aaaggaatga gaaatatgaa tacaccacaa
    17941 atttttaatt tcgagcaaaa cgaagttcga actattttag taaatgatga accatatttt
    18001 gtaggaaaag atgtagcaga cgttctagga tattcgaatc cgcaaaaagc tattcgagat
    18061 catgtggact tggaggataa gacgcagaac gattcgttca ccgtcaatgg aacagcagtt
    18121 gttttgatta acgagtcagg cctttacagt ttaatcttaa aatcaaaact tccctctgcc
    18181 aaaaaattta aacgttgggt aacgagtgaa gtccttccaa caattagaaa aacaggtagc
    18241 tattcaaacg tacctcaaag ttttgcacaa gcattgcgtt tagcagcgga tttagaagaa
    18301 aagaaccaat tactcgaaca acaaattgcc gagtacgaac caaagattag ctacttagat
    18361 acgattcttt catcgacaga tacggtagct acttctcaaa ttgcagctga ttacggaatg
    18421 tcggcaattg ctctaaacaa attgctaaac gagttaggtg ttcagcataa agttagcgga
    18481 caatggatac tttaccgaaa acatatgaac caaggataca caaaatcgca cacaagtgag
    18541 ataccgaaag ccgatggcgg cactaaagtt gtaatgaata ccaaatggac acagaaaggg
    18601 cgagtgttta tttacaactt attaatcgca gagggctatt accctcaaat ggatttagag
    18661 gaaattggtt agaaaggagt tttagtatga ctgacattgc agaaatcact caacgagata
    18721 gagaaaaaat caaagaatat gtcgaaagtt caaagttctt aacttacacc atgcttgctg
    18781 aaagatttgg aattagcaaa agctacttat ctttaatttt aaacggtaaa aagacttctg
    18841 cagaagcaaa cagaattata gattcgatta tcactatgta cgaattgtag agggaggtaa
    18901 agtcgttgga agataaaatc attcttacaa aatcagagct tcaagaaatg ttaaacatcg
    18961 agtatggaac gataattttt aggagggata ataagtgaaa aaaccaacac tttcagagct
    19021 gatagaagct gctgagaagg cagtaaaacc agacgactgg taccgacaaa gtttaatctt
    19081 ggagaagttc cacggcatgt caaaaactac tttagttgaa tactgcaagg aaatggaaac
    19141 aattcctgaa tttgcagaag gaatacttcg tccaggtcac tcaaccacat ttattcatta
    19201 ccatactttt atttggtttt taaagtggaa agacgcaaac aaatatcgtg taaaaagttt
    19261 atcgccttca gatgttttga aggaggcaag ttaatgggca aattcaacag agcactagta
    19321 ttcagcgcac cgctaatcat ctacgcttta ggcctctggg gaagcagaca agcgttgata
    19381 gggacaatcg tttacatggt ttggattttt atggggcttg atgaagctga gcacagagcg
    19441 aaaaaagcca gtcgggaggg actgactaaa atatgatttt tttatttttg ttcctttggg
    19501 ctgctattgc gtacttcatc cagctccttg ttgtcaaagt tgctcttatt tcttttagga
    19561 agaacaaaag aaaatagcgc tataaatgaa agataagtag ccgctacatt aaactgttct
    19621 tttgaaaaag ttacagtcat cggaatgata attgatattg aggaagtaat cttaattaaa
    19681 aatatagcaa tattttcaac atcgcgttgg tttttattta acattgtcca acatgtttta
    19741 gcaacaataa aaataattac acatgatatt agcacacaga ctgaaaaaag ttttagaaat
    19801 gcgatgtagt cagtgttatt gaactccttc aaaatactat ttttattcaa tgtaataaaa
    19861 ggaatttttt taattgaaga caggaaagat aaggtaataa acagaattat atatatggtt
    19921 aaagtcgcag taagaatgta attgagcttt ttcctcatag aatcacctca ctttctaacg
    19981 aaaattatac caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa
    20041 gaagctgtaa ctacgagctt acaagtggca gaaagtttca aaaagaaaca taagcatgta
    20101 ctagaagcaa ttgaatcaat aaaaagatcg gtcgaaaatt cggccaatgt tgaagatggg
    20161 tccaattttg gacagatgtt tgtggaaggg aacgagccag actcatacgg aagaagtcgg
    20221 agagtttatt tcatgaatag agacggattt tccttgctag ctatgggatt cactggaagt
    20281 aaagcaataa atttcaaact aaaatttatt gaagctttca acgaaatgga agatgttatt
    20341 cggaagaata ctgttcctca aacaattgaa gacatgatga tctatcaatt agaagaaatg
    20401 aaagatgtta aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa
    20461 gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg aagttctagg gggaaaagaa
    20521 agccgagctt atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa
    20581 cgtacctttt caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt
    20641 tcatttattg aaatgtggtt accagaaact gcgatccgca tggaaatcga tcaactgaac
    20701 agacaacaaa gacttttcgg tgatgaaaat gaatagagct gaagcgctaa gaatagggac
    20761 ggtaattgct aatcgctggt ggagacacaa taaaccaaac atcctaagcc aacaacatat
    20821 cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc agccggcaag catagagtca
    20881 caaaacatat ctaaggagaa ttttagcata tgaataaaga actttccact ttagatcaat
    20941 atttgactga ttctgaatgg ggcaagtcga atatcaagga aacaaataat cgaaaaatca
    21001 gacgaaatct tttgacgaac gaagaactat catgtgatca agatgacttg ggaaattttg
    21061 tgagtatttg ggatcatgtt taccttatcc atctatcaaa acattcaaat aaacctgaat
    21121 acatctatgt catcgaagat ggcttgattg atgcgctaga agagtacaac agagataact
    21181 tgattgatat ctcttattac ggatcaggta agaaatacat tgctgaaatg gaggcagaat
    21241 ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg taagttaaat
    21301 aaaattctca agaaaaaagg aaattttgat tatttatctt gggctcacgc gtgggagatt
    21361 atgaaaaaga atgatccaca ggcaacggta actattaatg agtataaaca ctacagggtt
    21421 gtttctggaa ctcatcaaga ctttcttgtt gaggaatata aaccttttct tatggatgaa
    21481 acagggactt atgtatctgt ctcagtaacg gttaaaggac atacggaaac agagttattt
    21541 cctgttttag attatcgaaa ccaaccagtt gttaaaccaa atgcaatgca aatcaataac
    21601 tcattgaagc gatgctttgt gaaagcattg gctctacacg gactgggatt atatgtattt
    21661 caaggggaag atattccaac accacctaga atcgatacaa agaaattaaa catgctagag
    21721 acgattctag aagctttcaa tgagcagatg ggtaaagata tgaccaaaac cttaatcgaa
    21781 tatgttaatg agcagacaga taaattaggg ctcttagctg ataacgttga aactattgaa
    21841 cagttaagct atgagcaatg tgccttgatg gagcgagcaa tagcagctaa gagaaaagaa
    21901 ttagataaga agtgatatga gtgtttaaac cattaatcga ttcatattca gcggttctga
    21961 aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga ggaagtgaac attgatcgac
    22021 taaagacgat gtatgacggc tacgatggtg atcgagtcat tgaaattcgt tttattgatc
    22081 ctagacgttt caccgtacag caacgaaact tcatctatgc gctgataggc gatattttta
    22141 tcgatacagg catgccaacg gacttctgga aggaattctt ctacttccgt
//
LOCUS       DADOOS010000034        22143 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.034, whole genome shotgun sequence.
ACCESSION   DADOOS010000034 DADOOS010000000
VERSION     DADOOS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.034"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..107
                     /locus_tag="J8C37_001100"
     CDS             <1..107
                     /locus_tag="J8C37_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5304556.1"
                     /translation="SNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(178..1488)
                     /locus_tag="J8C37_001101"
     CDS             complement(178..1488)
                     /locus_tag="J8C37_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAZ5304557.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1500..2918)
                     /locus_tag="J8C37_001102"
     CDS             complement(1500..2918)
                     /locus_tag="J8C37_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5304558.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3158..4726)
                     /locus_tag="J8C37_001103"
     CDS             complement(3158..4726)
                     /locus_tag="J8C37_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAZ5304559.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4730..5011)
                     /locus_tag="J8C37_001104"
     CDS             complement(4730..5011)
                     /locus_tag="J8C37_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAZ5304560.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5008..5931)
                     /locus_tag="J8C37_001105"
     CDS             complement(5008..5931)
                     /locus_tag="J8C37_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAZ5304561.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5928..6806)
                     /locus_tag="J8C37_001106"
     CDS             complement(5928..6806)
                     /locus_tag="J8C37_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAZ5304562.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6793..8130)
                     /locus_tag="J8C37_001107"
     CDS             complement(6793..8130)
                     /locus_tag="J8C37_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAZ5304563.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8133..9236)
                     /locus_tag="J8C37_001108"
     CDS             complement(8133..9236)
                     /locus_tag="J8C37_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAZ5304564.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9310..10818)
                     /locus_tag="J8C37_001109"
     CDS             complement(9310..10818)
                     /locus_tag="J8C37_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ5304565.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10784..11476)
                     /locus_tag="J8C37_001110"
     CDS             complement(10784..11476)
                     /locus_tag="J8C37_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ5304566.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11498..12472)
                     /locus_tag="J8C37_001111"
     CDS             complement(11498..12472)
                     /locus_tag="J8C37_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304567.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12772..13644
                     /locus_tag="J8C37_001112"
     CDS             12772..13644
                     /locus_tag="J8C37_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304568.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14226..16424)
                     /locus_tag="J8C37_001113"
     CDS             complement(14226..16424)
                     /locus_tag="J8C37_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304569.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16656..17648
                     /locus_tag="J8C37_001114"
     CDS             16656..17648
                     /locus_tag="J8C37_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5304570.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17708..19129
                     /locus_tag="J8C37_001115"
     CDS             17708..19129
                     /locus_tag="J8C37_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAZ5304571.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19122..20621
                     /locus_tag="J8C37_001116"
     CDS             19122..20621
                     /locus_tag="J8C37_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAZ5304572.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20660..22054
                     /gene="uxaC"
                     /locus_tag="J8C37_001117"
     CDS             20660..22054
                     /gene="uxaC"
                     /locus_tag="J8C37_001117"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAZ5304573.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6716 a         4514 c         3836 g         7077 t
ORIGIN      
        1 tcagcaatca acgtaatcat attccaagaa aatcattgaa ttacagaaca ccaatcgaaa
       61 tatttttgag ctatgtacaa gaagcatttt attctagctt aatttgacaa atcatatatc
      121 attaaaaaaa ataaaggtca agctaaaata ttatttaagc ttgaccttat tataatttca
      181 acgattgtca aaagtattgt ttgcagctgt gttgccagtt tctatagata aatcacagct
      241 tttgaaacat aagttttgta ctgcttcgat tcgcccgaat tcttttgcag ttatttggca
      301 atgagaaaag cagacatctt ctataggctt ttcaggaaat gctactaaat caaaagctcg
      361 ggaaggaagt gagtaacctg gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt
      421 gactttcgtc atttgctttg attcaggtat ttcttcagcc actgcttgcc aactttctgg
      481 catttgagca aggtcattgc tattttttct attgtattca ttatgccagt ccattatcca
      541 agaaaaagga aattgtacat caatcatatt cagattttct accctgatat tttcgatcac
      601 tccccctctt tcttttgaag atttcattcg aataccacag tctgactgaa aaaaattgat
      661 atcgtgaata tatacgtcac taactccagc actaacttcg ctaccaatag ttacaccgta
      721 tcctgaacgg attttacatc tagcaacctc gattcttgat gattttcgat tgactcttgc
      781 accattgcca tctctgccag acttaatagc aatacaatca tcaccacagg ccaaatcaca
      841 ttcatagaca cgcacatttg ttgaagaatc aatatcaatc ccatctgtac ttggtccatc
      901 attgttgcgg atgattactt tttctactaa tacatcgtta gaataagtga tttgcaagtt
      961 ccaaaaacct gatttctgaa aaatcaaatc acgaacaaca acatgttctg catgatacag
     1021 taaacaagcg cgtggacgtt taatagcata atccgcaatc caacgaagtc ccttttgatc
     1081 gtattcagct cttgtcccgc ttttttgatc ttttccccaa tacagctccc accaatggtc
     1141 tccttgaccg tcgattattc cttttccttc tataagaata tctttcgccg agagaacatt
     1201 caaaatcgct gctggccatt tcatttctac gccggctact cgtgtatcga tttctggaaa
     1261 tgcttcgata tctgttctcc ccttgaggac tgcaccttct tcaaagataa gatgactctt
     1321 atcttttaaa aaaagggctc cgactaaata aatgcctttt ggaataaata caggtaagtg
     1381 ttctttcgca ccttgatcga ttgctttttg cagtattatt gtatcatctt gttgagtgtc
     1441 gtttgtttta aagttcaaag cattgataat cccttttttt gtccgcaata cgtttctccc
     1501 tatctttcat tgattttttt caattcttca ccaataaata cagtttttga acgattcagt
     1561 gggtaaccta ctagcaaaat gattgcggat acaaataagc agaatgctgg taacaagttt
     1621 gccatcgtat aaatcctttc ttgtactaaa actgattgga tggctccacc tgttgtagac
     1681 gattggtaac caatgagtgc cagcataaat ccgcctaccg ctcctgcaca tgcttgtcct
     1741 acttttctcg caaaagaatt tactccatat actgttccat cttctcttaa gcctgtctta
     1801 tattgatgat aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt gaataaacca
     1861 gatcctaaag tagcgataaa taaaagaatc agatataccc atgcatttgt tatatgaagg
     1921 aaatacatca gcaagtacat acatgaagaa gcaagtaacg caaatacgct tgcttccttt
     1981 ttaccaaata attttgtcag ctttgtagca aatggtgcta aaactactac tgttccgtaa
     2041 gtaaataaaa gagcaacaga cattgcttgt ttattatgga agtaatcatt gaataaataa
     2101 gtcatattcg tcccagctaa aatctgattg atcacaatga aaatgtcaac aatgactaaa
     2161 ataatcagtg ctcgattagc taacaaacct tttaccaact gagaagcaga tactttttcg
     2221 tttttgacaa ctcttactct ttcagtagtc aatttacaag ttaacatata tgcgacaaat
     2281 gctattgcag cacatccgat tgaaatccaa aagaaatgat tccctgaaac gacttgttgt
     2341 ccactcttgt tatgggtata cattaaaatc ggaatgaaga atcctgtagt tgatccgcca
     2401 atcgcagaac caatgcttcg gaacgtggac aacgaggtgc ggtcattagg atttgagcta
     2461 attgctgctg ccattgctcc atacgggata ttgattgttg agagaaaaat cccataaaca
     2521 atgtatgaaa caaaaatata agcaatcttc accgtcattg gaagatgttg gacgaatggc
     2581 aaaaacaaaa tgacagtcac taagcaaaat ggatacttca tttttctgat ccaaggaata
     2641 aatcggccag tttctgttaa ctctgagaca tcacataatc ttccaaccat cacatcagca
     2701 aacgcatcca aaattctcga aatcaagaat aacgtaccta cgattgcccc tgaaacgcca
     2761 agaacattcg tataataaat catcaagaaa ctattgacta aacctaaaat aaaacagtta
     2821 cctagatcac cgaacatgta gcctatctta tcggaaatcc caaattcctt tttttctttt
     2881 tttactgtat ttgctgtaac agcattttgt gaaagcattt gaattcctcc tacctcttct
     2941 agtaaacgtt ttcaaaatta taactctttt tttcttgttt ttatattcac aatgttggct
     3001 ttttattgca ttatcttgac agataaaaaa caaaaaataa tctaaaaaca ttgatgcata
     3061 agattgataa ctttttttac ataacaatat atacaaacaa aaaaattcac taactagtga
     3121 aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct tttccatcat
     3181 acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca tgcactgcta
     3241 tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt tgcggaccta
     3301 ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta
     3361 cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct tctatttgtg
     3421 ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca ttccaagtaa
     3481 cataacaata aatataattg gattcattta agtacagtac cagtccagcc atctgattaa
     3541 aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa
     3601 tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc agacgtaaaa
     3661 aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt ctggcattcc
     3721 agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt tcttgttgta
     3781 cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta tctttaagtc
     3841 ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt cccaaaatag
     3901 ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct gacggggttt
     3961 gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca ctagctgtca
     4021 gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct cggcaaactg
     4081 taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa tacccgttac
     4141 gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat attttataga
     4201 tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata ccgctggatt
     4261 tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt tttcccgttt
     4321 caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt ggttcggacc
     4381 aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta aacggacggc
     4441 ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa ctgatctgtg
     4501 gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat
     4561 ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt cctggaagcc
     4621 attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata ttaggatctg
     4681 gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt caggctcctt
     4741 ttctcattct tactcgatat aaactgaatg ccccagctgc taaaaaagca agtgaaataa
     4801 acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag
     4861 acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca
     4921 tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca
     4981 ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat
     5041 ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt
     5101 ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat
     5161 cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt
     5221 tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt
     5281 tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg
     5341 acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca
     5401 gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg
     5461 agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga
     5521 tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc
     5581 taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga
     5641 tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc
     5701 ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt
     5761 ggataatctg ctttctatat attcatcata tcttccttct tttactatca agacctttgg
     5821 atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt
     5881 cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc
     5941 cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa
     6001 gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt
     6061 ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga
     6121 cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt
     6181 ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag tgtttcgatg
     6241 tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac
     6301 cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct
     6361 tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac
     6421 gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct
     6481 tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca
     6541 aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa
     6601 tctccgccgt gagaatgcag gtaaaggacc gtcggaaacg gtccttcttt ttctggataa
     6661 gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata
     6721 aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga
     6781 gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat
     6841 attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag
     6901 aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa
     6961 gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc
     7021 gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa
     7081 ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt
     7141 cgaaaaatgg atccttctga atgctgatgt ccgttcatct atcggataag cctttttttc
     7201 ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg
     7261 acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg
     7321 acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat
     7381 ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg
     7441 gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc
     7501 agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg
     7561 cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa
     7621 gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg
     7681 tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa
     7741 tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt
     7801 tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata
     7861 gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata
     7921 atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt
     7981 gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc
     8041 tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat
     8101 tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct
     8161 tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa
     8221 gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc
     8281 aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat
     8341 ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc
     8401 attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt
     8461 tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc
     8521 agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca
     8581 taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat
     8641 agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc
     8701 aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct
     8761 tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata
     8821 tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg
     8881 gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt
     8941 tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata
     9001 ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata
     9061 ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac
     9121 cctttaagta cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat
     9181 aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt
     9241 tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa
     9301 tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc
     9361 gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc
     9421 catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt
     9481 gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt
     9541 gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc
     9601 actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa
     9661 ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa
     9721 tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc
     9781 ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga
     9841 ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat
     9901 atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta
     9961 gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa
    10021 gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata
    10081 ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg
    10141 tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg
    10201 atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg
    10261 tttagctact tcataaagct catccatggt agttggaaca tctagtccta gattatccaa
    10321 ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta
    10381 caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt
    10441 atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac
    10501 agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc
    10561 caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc
    10621 cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg
    10681 caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact
    10741 tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac
    10801 gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt
    10861 ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc
    10921 acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt
    10981 ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat
    11041 gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat
    11101 gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca
    11161 aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga
    11221 tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga
    11281 agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa
    11341 ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc
    11401 cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg tttattttat
    11461 ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta
    11521 gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca
    11581 atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg
    11641 tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga
    11701 accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa
    11761 ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag
    11821 cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc
    11881 ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta
    11941 gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa
    12001 cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt
    12061 aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag
    12121 acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat
    12181 ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga
    12241 cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc
    12301 cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat
    12361 ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt
    12421 cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct
    12481 ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg
    12541 tatataatca tttttatcgt aagtaaaaac gcttacatag caatgatttt tgtgctatac
    12601 aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt
    12661 ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg
    12721 aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag
    12781 aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta
    12841 gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa
    12901 gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc
    12961 tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt
    13021 gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag
    13081 tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg
    13141 gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc
    13201 atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa
    13261 gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta
    13321 tttttcaaaa ttatgctgcc attagcttta ccgtctattg ctactatctc gcttttttat
    13381 gctgttactt actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa
    13441 tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa
    13501 agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt
    13561 gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca
    13621 ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa
    13681 gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa
    13741 atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa
    13801 gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac
    13861 aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta
    13921 ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt
    13981 ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac
    14041 tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga
    14101 ttaaacggcg tttatcttgt tgattctgcg gcaataaaat cagccagcag caaaacatga
    14161 agtgctgttt tttgctgtcc tcttttattg cttgaatcag atcacaagaa aaacagcctc
    14221 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14281 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14341 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14401 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14461 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14521 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14581 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14641 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14701 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14761 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    14821 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    14881 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    14941 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15001 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15061 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15121 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15181 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15241 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15301 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15361 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15421 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15481 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15541 aatctgtccg aaaataagta ggaaaaaaga ccaatgagtc ctaccagtgt taaagacact
    15601 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15661 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15721 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15781 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    15841 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    15901 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    15961 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaact
    16021 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16081 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16141 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16201 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16261 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16321 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16381 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16441 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16501 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16561 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16621 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatatcgcta
    16681 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16741 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16801 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    16861 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    16921 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    16981 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17041 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17101 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17161 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17221 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17281 tagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17341 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17401 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17461 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17521 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17581 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17641 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17701 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17761 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    17821 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    17881 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    17941 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18001 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18061 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18121 acagagcttc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18181 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18241 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18301 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18361 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18421 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18481 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18541 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18601 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18661 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18721 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18781 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    18841 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    18901 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    18961 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19021 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19081 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19141 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19201 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19261 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19321 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19381 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19441 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19501 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19561 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19621 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19681 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19741 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19801 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    19861 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    19921 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    19981 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20041 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20101 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20161 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20221 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20281 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20341 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20401 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20461 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20521 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20581 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20641 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20701 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20761 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    20821 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    20881 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    20941 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21001 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21061 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21121 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21181 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21241 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21301 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21361 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21421 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcttacaga
    21481 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21541 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21601 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21661 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21721 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21781 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    21841 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    21901 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    21961 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22021 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22081 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22141 caa
//
LOCUS       DADOOS010000035        22031 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.035, whole genome shotgun sequence.
ACCESSION   DADOOS010000035 DADOOS010000000
VERSION     DADOOS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.035"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            367..555
                     /locus_tag="J8C37_001118"
     CDS             367..555
                     /locus_tag="J8C37_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304574.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="J8C37_001119"
     CDS             545..805
                     /locus_tag="J8C37_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304575.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="J8C37_001120"
     CDS             846..1073
                     /locus_tag="J8C37_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304576.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="J8C37_001121"
     CDS             1138..1710
                     /locus_tag="J8C37_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304577.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="J8C37_001122"
     CDS             1711..2022
                     /locus_tag="J8C37_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAZ5304578.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="J8C37_001123"
     CDS             2013..2273
                     /locus_tag="J8C37_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304579.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="J8C37_001124"
     CDS             2434..2793
                     /locus_tag="J8C37_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304580.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="J8C37_001125"
     CDS             2790..4433
                     /locus_tag="J8C37_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAZ5304581.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4447..5550
                     /locus_tag="J8C37_001126"
     CDS             4447..5550
                     /locus_tag="J8C37_001126"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304582.1"
                     /translation="MKGVVIEVKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFT
                     TKVDDSVTSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAEN
                     ERAKKELLTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSK
                     DLSDSEIGSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILN
                     EQYEKMQIYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVI
                     LFNSLYIKRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKR
                     QKLSEYIKELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="J8C37_001127"
     CDS             5594..6766
                     /locus_tag="J8C37_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAZ5304583.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="J8C37_001128"
     CDS             6753..7457
                     /locus_tag="J8C37_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAZ5304584.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="J8C37_001129"
     CDS             7462..8622
                     /locus_tag="J8C37_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAZ5304585.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="J8C37_001130"
     CDS             8698..8976
                     /locus_tag="J8C37_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAZ5304586.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="J8C37_001131"
     CDS             8957..9328
                     /locus_tag="J8C37_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAZ5304587.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="J8C37_001132"
     CDS             9344..9748
                     /locus_tag="J8C37_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304588.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="J8C37_001133"
     CDS             9745..10155
                     /locus_tag="J8C37_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304589.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="J8C37_001134"
     CDS             10216..10803
                     /locus_tag="J8C37_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAZ5304590.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="J8C37_001135"
     CDS             10876..11343
                     /locus_tag="J8C37_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAZ5304591.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="J8C37_001136"
     CDS             11389..11697
                     /locus_tag="J8C37_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304592.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="J8C37_001137"
     CDS             11892..18626
                     /locus_tag="J8C37_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAZ5304593.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="J8C37_001138"
     CDS             18630..19502
                     /locus_tag="J8C37_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304594.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20126
                     /locus_tag="J8C37_001139"
                     /pseudo
     CDS             19499..20126
                     /locus_tag="J8C37_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20087..20548
                     /locus_tag="J8C37_001140"
     CDS             20087..20548
                     /locus_tag="J8C37_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304595.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="J8C37_001141"
     CDS             20552..21376
                     /locus_tag="J8C37_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304596.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..21939
                     /locus_tag="J8C37_001142"
                     /pseudo
     CDS             21394..21939
                     /locus_tag="J8C37_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DADOOS010000036        21988 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.036, whole genome shotgun sequence.
ACCESSION   DADOOS010000036 DADOOS010000000
VERSION     DADOOS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.036"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..395
                     /locus_tag="J8C37_001143"
     CDS             <1..395
                     /locus_tag="J8C37_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304597.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="J8C37_001144"
     CDS             398..1147
                     /locus_tag="J8C37_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304598.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="J8C37_001145"
     CDS             1159..1428
                     /locus_tag="J8C37_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304599.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="J8C37_001146"
     CDS             1590..1892
                     /locus_tag="J8C37_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304600.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="J8C37_001147"
     CDS             1889..2050
                     /locus_tag="J8C37_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAZ5304601.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="J8C37_001148"
     CDS             2047..2352
                     /locus_tag="J8C37_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304602.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="J8C37_001149"
     CDS             2352..2708
                     /locus_tag="J8C37_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAZ5304603.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="J8C37_001150"
     CDS             2668..2913
                     /locus_tag="J8C37_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304604.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="J8C37_001151"
     CDS             2910..3329
                     /locus_tag="J8C37_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAZ5304605.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="J8C37_001152"
     CDS             3326..3883
                     /locus_tag="J8C37_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAZ5304606.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="J8C37_001153"
     CDS             3880..4176
                     /locus_tag="J8C37_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304607.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="J8C37_001154"
     CDS             4253..4666
                     /locus_tag="J8C37_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAZ5304608.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="J8C37_001155"
     CDS             5012..5386
                     /locus_tag="J8C37_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304609.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="J8C37_001156"
     CDS             5616..5960
                     /locus_tag="J8C37_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAZ5304610.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="J8C37_001157"
     CDS             5965..6246
                     /locus_tag="J8C37_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304611.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="J8C37_001158"
     CDS             6349..6663
                     /locus_tag="J8C37_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAZ5304612.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="J8C37_001159"
     CDS             6641..8335
                     /locus_tag="J8C37_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAZ5304613.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="J8C37_001160"
     CDS             8355..9533
                     /locus_tag="J8C37_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAZ5304614.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="J8C37_001161"
     CDS             9496..10182
                     /locus_tag="J8C37_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAZ5304615.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="J8C37_001162"
     CDS             10182..11342
                     /locus_tag="J8C37_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAZ5304616.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="J8C37_001163"
     CDS             11352..12227
                     /locus_tag="J8C37_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304617.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="J8C37_001164"
     CDS             12224..12535
                     /locus_tag="J8C37_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304618.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="J8C37_001165"
     CDS             12525..12878
                     /locus_tag="J8C37_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAZ5304619.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="J8C37_001166"
     CDS             12868..13269
                     /locus_tag="J8C37_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304620.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="J8C37_001167"
     CDS             13262..13666
                     /locus_tag="J8C37_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304621.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="J8C37_001168"
     CDS             13678..14286
                     /locus_tag="J8C37_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAZ5304622.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="J8C37_001169"
     CDS             14306..14668
                     /locus_tag="J8C37_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304623.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="J8C37_001170"
     CDS             14671..14853
                     /locus_tag="J8C37_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304624.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="J8C37_001171"
     CDS             14870..18289
                     /locus_tag="J8C37_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAZ5304625.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="J8C37_001172"
     CDS             18340..19077
                     /locus_tag="J8C37_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAZ5304626.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="J8C37_001173"
     CDS             19087..21378
                     /locus_tag="J8C37_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304627.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="J8C37_001174"
     CDS             21402..>21988
                     /locus_tag="J8C37_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304628.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DADOOS010000037        21909 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.037, whole genome shotgun sequence.
ACCESSION   DADOOS010000037 DADOOS010000000
VERSION     DADOOS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.037"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="J8C37_001175"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="J8C37_001175"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAZ5304629.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="J8C37_001176"
     CDS             complement(1172..1867)
                     /locus_tag="J8C37_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAZ5304630.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="J8C37_001177"
     CDS             complement(1880..2596)
                     /locus_tag="J8C37_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAZ5304631.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="J8C37_001178"
     CDS             2926..3534
                     /locus_tag="J8C37_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAZ5304632.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="J8C37_001179"
     CDS             3859..4182
                     /locus_tag="J8C37_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAZ5304633.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="J8C37_001180"
     CDS             4364..5734
                     /locus_tag="J8C37_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAZ5304634.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="J8C37_001181"
     CDS             complement(5864..7165)
                     /locus_tag="J8C37_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAZ5304635.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="J8C37_001182"
     CDS             complement(7519..9510)
                     /locus_tag="J8C37_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAZ5304636.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="J8C37_001183"
     CDS             complement(9654..9920)
                     /locus_tag="J8C37_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ5304637.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="J8C37_001184"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="J8C37_001184"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAZ5304638.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="J8C37_001185"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="J8C37_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAZ5304639.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="J8C37_001186"
     CDS             13264..13827
                     /locus_tag="J8C37_001186"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAZ5304640.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="J8C37_001187"
     CDS             complement(14080..14601)
                     /locus_tag="J8C37_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAZ5304641.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="J8C37_001188"
     CDS             complement(14604..15446)
                     /locus_tag="J8C37_001188"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAZ5304642.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="J8C37_001189"
     CDS             complement(15439..16164)
                     /locus_tag="J8C37_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAZ5304643.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="J8C37_001190"
     CDS             16340..16636
                     /locus_tag="J8C37_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304644.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="J8C37_001191"
     CDS             17204..18796
                     /locus_tag="J8C37_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ5304645.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="J8C37_001192"
     CDS             18809..19549
                     /locus_tag="J8C37_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304646.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="J8C37_001193"
     CDS             19717..21066
                     /locus_tag="J8C37_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5304647.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="J8C37_001194"
     CDS             complement(21194..21784)
                     /locus_tag="J8C37_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAZ5304648.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6292 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaa
//
LOCUS       DADOOS010000038        20979 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.038, whole genome shotgun sequence.
ACCESSION   DADOOS010000038 DADOOS010000000
VERSION     DADOOS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.038"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..1406
                     /gene="rseP"
                     /locus_tag="J8C37_001195"
     CDS             138..1406
                     /gene="rseP"
                     /locus_tag="J8C37_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAZ5304649.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1474..3183
                     /locus_tag="J8C37_001196"
     CDS             1474..3183
                     /locus_tag="J8C37_001196"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAZ5304650.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3395..7753
                     /locus_tag="J8C37_001197"
     CDS             3395..7753
                     /locus_tag="J8C37_001197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAZ5304651.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7898..8371
                     /gene="rimP"
                     /locus_tag="J8C37_001198"
     CDS             7898..8371
                     /gene="rimP"
                     /locus_tag="J8C37_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAZ5304652.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8394..9569
                     /gene="nusA"
                     /locus_tag="J8C37_001199"
     CDS             8394..9569
                     /gene="nusA"
                     /locus_tag="J8C37_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAZ5304653.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9591..9884
                     /locus_tag="J8C37_001200"
     CDS             9591..9884
                     /locus_tag="J8C37_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAZ5304654.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9881..10192
                     /locus_tag="J8C37_001201"
     CDS             9881..10192
                     /locus_tag="J8C37_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAZ5304655.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10205..12511
                     /gene="infB"
                     /locus_tag="J8C37_001202"
     CDS             10205..12511
                     /gene="infB"
                     /locus_tag="J8C37_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAZ5304656.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12538..12885
                     /gene="rbfA"
                     /locus_tag="J8C37_001203"
     CDS             12538..12885
                     /gene="rbfA"
                     /locus_tag="J8C37_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAZ5304657.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13042..13215)
                     /locus_tag="J8C37_001204"
     CDS             complement(13042..13215)
                     /locus_tag="J8C37_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304658.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13663..16025
                     /locus_tag="J8C37_001205"
                     /pseudo
     CDS             13663..16025
                     /locus_tag="J8C37_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16393..17316
                     /gene="truB"
                     /locus_tag="J8C37_001206"
     CDS             16393..17316
                     /gene="truB"
                     /locus_tag="J8C37_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAZ5304659.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17320..18261
                     /gene="ribF"
                     /locus_tag="J8C37_001207"
     CDS             17320..18261
                     /gene="ribF"
                     /locus_tag="J8C37_001207"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAZ5304660.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18613..19398
                     /locus_tag="J8C37_001208"
     CDS             18613..19398
                     /locus_tag="J8C37_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5304661.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19411..20262
                     /locus_tag="J8C37_001209"
     CDS             19411..20262
                     /locus_tag="J8C37_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAZ5304662.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20386..20838)
                     /locus_tag="J8C37_001210"
     CDS             complement(20386..20838)
                     /locus_tag="J8C37_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304663.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7182 a         3688 c         4571 g         5538 t
ORIGIN      
        1 gaatttcata aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg
       61 gtatactatg gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag
      121 taaaggagaa aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag
      181 tgattgttca tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg
      241 aatttgctat cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat
      301 atacgcttcg cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg
      361 aaacagaaat ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag
      421 agaagataaa tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc
      481 gttatgattt agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag
      541 tagtaaccta ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca
      601 gaattgcgcc aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca
      661 actttgctgg acccatgaat aactttattt tggcaatcct gttattcatc attctggcat
      721 ttatgcaagg tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg
      781 gagctgcggc agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag
      841 aaatccattc atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag
      901 actttaagat cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag
      961 agtcaaatgg cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta
     1021 tggataagat cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag
     1081 ccttaggatc actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt
     1141 accaattgtc ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc
     1201 ttctttcaat gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga
     1261 aattagtctt gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag
     1321 gaatattgac attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga
     1381 acgatattca acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga
     1441 taactatttt gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca
     1501 acattacgcg aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct
     1561 ggatatatcc gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta
     1621 ttggaaaaac tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg
     1681 ctgatgcctg ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat
     1741 ggaccaaact tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact
     1801 cacgaagaaa cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct
     1861 ttaaatcttt atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg
     1921 cttcgcggaa gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt
     1981 ttggacgaga catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc
     2041 ttagaatttc gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa
     2101 ttcatggcga tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat
     2161 gctgcaaact tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag
     2221 cttgaattgg agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc
     2281 ttttctgttg aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct
     2341 gtccttgttt tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta
     2401 tctgctgact ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag
     2461 tttggttctg tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat
     2521 gtccaagatt tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat
     2581 gtgaatccag aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa
     2641 ggagatcctt caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga
     2701 catatcttca aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa
     2761 aatggacgag aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta
     2821 tcagcaatcg tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg
     2881 ccatttgatt tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg
     2941 aatgaactag aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat
     3001 gaacgcgcag gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact
     3061 gtcggtaaaa aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg
     3121 gttgaagtac gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa
     3181 taataagcgt taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga
     3241 tgattcgctt ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa
     3301 actcagaaaa aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa
     3361 ttgcattgtt tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga
     3421 aaaactgttg gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc
     3481 tggtgaaatg gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca
     3541 tttttccaaa attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc
     3601 ttttaaagat attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga
     3661 acagctcttg caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact
     3721 agcacagcaa gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc
     3781 aatcgacagt acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga
     3841 gttgttcgtc agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca
     3901 agcggaacga gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat
     3961 gaagcaagca gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca
     4021 atcgccagct cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga
     4081 accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta
     4141 cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat
     4201 cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca
     4261 agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga
     4321 tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc
     4381 tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat
     4441 gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg
     4501 acaccgagct atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag
     4561 tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga
     4621 tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt
     4681 atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc
     4741 agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca
     4801 tcctttgtca cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc
     4861 aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt
     4921 agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat
     4981 tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca
     5041 attcaaacgt ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca
     5101 tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga
     5161 ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg
     5221 agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg
     5281 actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc
     5341 acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg
     5401 tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg
     5461 agcaaaattc tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga
     5521 gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat
     5581 cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga
     5641 agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg
     5701 tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt
     5761 tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat
     5821 ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga
     5881 agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc
     5941 tgagatcatt caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc
     6001 ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt
     6061 aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt
     6121 taatccgtta gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga
     6181 tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg
     6241 cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa
     6301 agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac
     6361 gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga
     6421 caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag
     6481 tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca
     6541 tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca
     6601 atatccagca gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat
     6661 tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat
     6721 gcttcaagat ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat
     6781 gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac
     6841 agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac
     6901 gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt
     6961 atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat
     7021 cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc
     7081 atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg
     7141 agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg
     7201 ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct
     7261 tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt
     7321 acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc
     7381 aatgaaagaa atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac
     7441 agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt
     7501 gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag
     7561 agctgtccca agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg
     7621 tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga
     7681 atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt
     7741 cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata
     7801 gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat
     7861 ggaattacgc acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt
     7921 cacagaaatg gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt
     7981 tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga
     8041 tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc
     8101 agatccgatt cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt
     8161 gaaaaaagaa agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca
     8221 accagtagat ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct
     8281 tacattgaaa atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat
     8341 tgcaaaagct cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta
     8401 aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg
     8461 tgatcgatgc tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac
     8521 aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag
     8581 aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa
     8641 tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg
     8701 ggcggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa
     8761 gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg
     8821 aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta
     8881 aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt
     8941 cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc
     9001 tattgcgccg cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg
     9061 tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa
     9121 atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca
     9181 atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca
     9241 ttgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa
     9301 aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac
     9361 aaaacgcacg tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg
     9421 acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg
     9481 aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg
     9541 aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa
     9601 ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg
     9661 tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg
     9721 gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa
     9781 ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact
     9841 atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca
     9901 aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga
     9961 actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc
    10021 aagcgagaat acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt
    10081 tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg
    10141 aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga
    10201 ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga
    10261 cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac
    10321 tagcaatgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc
    10381 tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga
    10441 aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg
    10501 gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac
    10561 cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa
    10621 caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa
    10681 taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa
    10741 attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc
    10801 gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat
    10861 ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg
    10921 catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc
    10981 agaagaagtc gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca
    11041 cgtagaccat gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg
    11101 cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc
    11161 aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc
    11221 aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac
    11281 agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa
    11341 aattgataaa ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt
    11401 aattcctgaa gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca
    11461 aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc
    11521 agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg
    11581 tcctgtgtca accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt
    11641 cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga
    11701 agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga
    11761 ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa
    11821 acgtgcatta ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga
    11881 aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg
    11941 ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa
    12001 aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag
    12061 caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga
    12121 tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga
    12181 aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat
    12241 cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga
    12301 aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga
    12361 aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta
    12421 tgaatgtggt gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg
    12481 atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg
    12541 gctaactacc gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg
    12601 cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg
    12661 ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc atcagataag
    12721 aaaacagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac
    12781 aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat
    12841 ggcaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca
    12901 aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag
    12961 agttccatga ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta
    13021 aaaaattcga ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa
    13081 gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt
    13141 aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt
    13201 taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca
    13261 cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat accaattcac
    13321 atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct
    13381 cgatccagcc ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat
    13441 gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg
    13501 atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt
    13561 ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta
    13621 tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa
    13681 gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa
    13741 ctttatctta aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa
    13801 catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac
    13861 cgagtcatca ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga
    13921 caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta
    13981 acggaagatg agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt
    14041 gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac
    14101 tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg
    14161 attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt
    14221 atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata
    14281 gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga
    14341 cttggctggg aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg
    14401 gctgaaaaaa tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg
    14461 aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa
    14521 ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac
    14581 caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg
    14641 ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc
    14701 agagaaatcg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga
    14761 atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa
    14821 ccaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt
    14881 gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg
    14941 aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat
    15001 gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa
    15061 gggttcttag aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat
    15121 tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg
    15181 acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc
    15241 agcaagatca caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa
    15301 agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca
    15361 tggataaagc attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc
    15421 gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca
    15481 ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa
    15541 cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac
    15601 taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc
    15661 cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat
    15721 ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc
    15781 atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg
    15841 tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa
    15901 gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata
    15961 ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc
    16021 aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa
    16081 aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt
    16141 tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc
    16201 aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta
    16261 cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg
    16321 tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa
    16381 tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat
    16441 gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg
    16501 cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc
    16561 gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca
    16621 acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct
    16681 gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca
    16741 atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa
    16801 acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg
    16861 acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga
    16921 acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg
    16981 tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa
    17041 gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa
    17101 ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac
    17161 ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct
    17221 ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa
    17281 ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga
    17341 catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc
    17401 gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa
    17461 gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag
    17521 atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc
    17581 gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca
    17641 ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt
    17701 tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc
    17761 aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc
    17821 tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta
    17881 ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta
    17941 ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt
    18001 tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat
    18061 aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat
    18121 caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg
    18181 aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct
    18241 gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc
    18301 aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc
    18361 acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa
    18421 atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact
    18481 agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg
    18541 tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa
    18601 ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca
    18661 aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc
    18721 aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc
    18781 ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt
    18841 gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa
    18901 aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat
    18961 ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa
    19021 aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct
    19081 cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat
    19141 gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat
    19201 gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga
    19261 cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc
    19321 agttatgtta caggtattac actatttgca gatggtggga tgactttata tccttctttc
    19381 caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca
    19441 gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag
    19501 atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct
    19561 ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt
    19621 attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca
    19681 atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa
    19741 tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt
    19801 tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt
    19861 agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt
    19921 gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg
    19981 atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg
    20041 atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat
    20101 ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt
    20161 atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta
    20221 ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat
    20281 ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg
    20341 ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga
    20401 aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt
    20461 gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag
    20521 acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg
    20581 attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag
    20641 catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa
    20701 aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc
    20761 atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac
    20821 tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat
    20881 atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa
    20941 gtcagaaaat tctaactttt atttctctaa agaaattcg
//
LOCUS       DADOOS010000039        20801 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.039, whole genome shotgun sequence.
ACCESSION   DADOOS010000039 DADOOS010000000
VERSION     DADOOS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.039"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..300
                     /locus_tag="J8C37_001211"
     CDS             <1..300
                     /locus_tag="J8C37_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ5304664.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="J8C37_001212"
     CDS             complement(724..894)
                     /locus_tag="J8C37_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5304665.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="J8C37_001213"
     CDS             complement(1088..1267)
                     /locus_tag="J8C37_001213"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304666.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1453)
                     /locus_tag="J8C37_001214"
     CDS             complement(1277..1453)
                     /locus_tag="J8C37_001214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304667.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="J8C37_001215"
     CDS             1654..2766
                     /locus_tag="J8C37_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ5304668.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="J8C37_001216"
     CDS             complement(2844..3137)
                     /locus_tag="J8C37_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAZ5304669.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="J8C37_001217"
     CDS             3305..3619
                     /locus_tag="J8C37_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAZ5304670.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="J8C37_001218"
     CDS             3640..5619
                     /locus_tag="J8C37_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ5304671.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="J8C37_001219"
     CDS             5632..5958
                     /locus_tag="J8C37_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAZ5304672.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="J8C37_001220"
     CDS             5974..6492
                     /locus_tag="J8C37_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304673.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="J8C37_001221"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="J8C37_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAZ5304674.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="J8C37_001222"
     CDS             complement(7928..9433)
                     /locus_tag="J8C37_001222"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAZ5304675.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="J8C37_001223"
     CDS             complement(9525..10115)
                     /locus_tag="J8C37_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ5304676.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="J8C37_001224"
     CDS             10294..11940
                     /locus_tag="J8C37_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ5304677.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="J8C37_001225"
     CDS             11989..12708
                     /locus_tag="J8C37_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAZ5304678.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="J8C37_001226"
     CDS             13187..14134
                     /locus_tag="J8C37_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304679.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="J8C37_001227"
     CDS             14146..15066
                     /locus_tag="J8C37_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304680.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="J8C37_001228"
     CDS             15107..16564
                     /locus_tag="J8C37_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304681.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="J8C37_001229"
     CDS             16909..17523
                     /locus_tag="J8C37_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ5304682.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="J8C37_001230"
     CDS             17516..19240
                     /locus_tag="J8C37_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5304683.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="J8C37_001231"
     CDS             19237..20688
                     /locus_tag="J8C37_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304684.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6708 a         3510 c         4167 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgag
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DADOOS010000040        19888 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.040, whole genome shotgun sequence.
ACCESSION   DADOOS010000040 DADOOS010000000
VERSION     DADOOS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.040"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(16..213)
                     /locus_tag="J8C37_001232"
     CDS             complement(16..213)
                     /locus_tag="J8C37_001232"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304685.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(359..1237)
                     /locus_tag="J8C37_001233"
     CDS             complement(359..1237)
                     /locus_tag="J8C37_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5304686.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1276..2394)
                     /locus_tag="J8C37_001234"
     CDS             complement(1276..2394)
                     /locus_tag="J8C37_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAZ5304687.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2391..4490)
                     /locus_tag="J8C37_001235"
     CDS             complement(2391..4490)
                     /locus_tag="J8C37_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAZ5304688.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4541..5362)
                     /locus_tag="J8C37_001236"
     CDS             complement(4541..5362)
                     /locus_tag="J8C37_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5304689.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5359..6168)
                     /locus_tag="J8C37_001237"
     CDS             complement(5359..6168)
                     /locus_tag="J8C37_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5304690.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6189..6683)
                     /locus_tag="J8C37_001238"
     CDS             complement(6189..6683)
                     /locus_tag="J8C37_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5304691.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6695..7183)
                     /locus_tag="J8C37_001239"
     CDS             complement(6695..7183)
                     /locus_tag="J8C37_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5304692.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7260..9956)
                     /locus_tag="J8C37_001240"
     CDS             complement(7260..9956)
                     /locus_tag="J8C37_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5304693.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10384..11457)
                     /locus_tag="J8C37_001241"
     CDS             complement(10384..11457)
                     /locus_tag="J8C37_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304694.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11454..12281)
                     /locus_tag="J8C37_001242"
     CDS             complement(11454..12281)
                     /locus_tag="J8C37_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304695.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12282..13088)
                     /locus_tag="J8C37_001243"
     CDS             complement(12282..13088)
                     /locus_tag="J8C37_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304696.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13100..14185)
                     /locus_tag="J8C37_001244"
     CDS             complement(13100..14185)
                     /locus_tag="J8C37_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304697.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14202..14744)
                     /locus_tag="J8C37_001245"
     CDS             complement(14202..14744)
                     /locus_tag="J8C37_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304698.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15002..15991
                     /locus_tag="J8C37_001246"
     CDS             15002..15991
                     /locus_tag="J8C37_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAZ5304699.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15988..16725
                     /locus_tag="J8C37_001247"
     CDS             15988..16725
                     /locus_tag="J8C37_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAZ5304700.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16722..17273
                     /gene="coaC"
                     /locus_tag="J8C37_001248"
     CDS             16722..17273
                     /gene="coaC"
                     /locus_tag="J8C37_001248"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAZ5304701.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17289..17921
                     /locus_tag="J8C37_001249"
     CDS             17289..17921
                     /locus_tag="J8C37_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ5304702.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18006..18653
                     /locus_tag="J8C37_001250"
     CDS             18006..18653
                     /locus_tag="J8C37_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5304703.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18658..19641
                     /locus_tag="J8C37_001251"
     CDS             18658..19641
                     /locus_tag="J8C37_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAZ5304704.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         6094 a         4270 c         3471 g         6053 t
ORIGIN      
        1 ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta tgaagtcaac
       61 atttcgaaaa atgaacatac attggtaagc aactataact attaccacag acataaagta
      121 acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg tatacagcgc
      181 acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta atgaaaacaa
      241 cacgatttga cttagagcct ttttatttga attcattttc gctttatcca acttcatttg
      301 atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca ttcattaatt
      361 atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt attccggcat
      421 tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca acatatccat
      481 ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt ttcattacac
      541 caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat gcacattggg
      601 caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc tggcctttta
      661 cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa tcttggtgga
      721 agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata tgccccattt
      781 ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct ccgatacctg
      841 ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca acatattcac
      901 catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa tattgcttta
      961 gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg atatgaccga
     1021 aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg atgcctgcca
     1081 attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca actggagttg
     1141 ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact gcacagacaa
     1201 atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc cttcgaatat
     1261 gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt
     1321 tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt gccgctcaaa
     1381 taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt cggtaaaaca
     1441 gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa
     1501 ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat ctctatttga
     1561 ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac gtccgaactc
     1621 atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg ttgaggttac
     1681 ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc cccaccaata
     1741 acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac cctttaatcg
     1801 atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg
     1861 aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg tataaaaaaa
     1921 tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg tttggaaagg
     1981 catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca acgagttcac
     2041 ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt caacagcagg
     2101 gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa aactgccaaa
     2161 ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc cagtaatatc
     2221 cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt tgcggcttgg
     2281 tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat tttcgggagt
     2341 gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac tcatcttttt
     2401 ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt cgtatcactt
     2461 attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt acattcaatc
     2521 gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt atcaacagga
     2581 tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat cattaggctg
     2641 acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac gacttctcct
     2701 tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc tgccaaacaa
     2761 attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct cattaaaaca
     2821 gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc tttgatttcc
     2881 tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg atataaatga
     2941 tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc ttctccatca
     3001 tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat taacccttgc
     3061 tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc atcaccgatt
     3121 ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca aattgattcc
     3181 tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc tagggcttca
     3241 aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc ggtatgatac
     3301 tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg ttcccctgac
     3361 cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac ctgcggacac
     3421 cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag taacgcactg
     3481 gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc aggaaactta
     3541 acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc aaacatggtg
     3601 aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc aggatagctg
     3661 ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga aaagctttga
     3721 taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag ctgcccaaat
     3781 agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga ttcctgaaaa
     3841 gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct tgccacttcc
     3901 tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg ctgatccgaa
     3961 tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc gtgtgtttca
     4021 ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag tatgggagca
     4081 aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg caaacagtct
     4141 tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac aatcccgttt
     4201 acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac ccaaacacct
     4261 ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat cgtcccgcaa
     4321 acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc ctttacatca
     4381 ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc gtttaactca
     4441 gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat cttttccctc
     4501 cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt ccaagtaatg
     4561 acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg acttttttat
     4621 ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta aaaatactat
     4681 cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc aaaacggtat
     4741 tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag atactagcag
     4801 cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat ttagagccta
     4861 atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata ttgaacataa
     4921 ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc gctagtcccg
     4981 cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc aatggtccca
     5041 tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt tcagaatttt
     5101 ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa
     5161 aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg tagaaatgat
     5221 taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc ggattcaccg
     5281 aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc tcatctttag
     5341 aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa gcgttgcatt
     5401 tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta gatctctttg
     5461 ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag
     5521 atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac tccaaagcat
     5581 ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg ttaatccgtt
     5641 catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc caaataaaac
     5701 ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa agaagtgcag
     5761 attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag cagccatcgg
     5821 tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg ctagtggaaa
     5881 gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg ctgtagccag
     5941 cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga cacccataaa
     6001 gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc
     6061 tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca tttcatgtcc
     6121 agcaattcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa tttttctcct
     6181 tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat cgccggtgta
     6241 ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg aatttctgac
     6301 gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg tgtcattttc
     6361 ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc aacttttacc
     6421 gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg cggatctttt
     6481 aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga tggtgcagcc
     6541 atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc tactactaac
     6601 ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat taatcgttca
     6661 tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc tcttcattta
     6721 ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta tttaccagtt
     6781 cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc agaattggca
     6841 gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt ttggtaatct
     6901 tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc tgctcgttca
     6961 cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca taagcattaa
     7021 tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga aagcctgaag
     7081 ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct ttctttctgt
     7141 tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta atcacgttaa
     7201 ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt ttagacactt
     7261 tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata agcattgact
     7321 ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata ataaaattca
     7381 tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg agccaagaat
     7441 aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg gccatttaaa
     7501 gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact catcacgatc
     7561 gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa ttttactagc
     7621 tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat atgctccaaa
     7681 ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat tgaaaaggaa
     7741 cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt ttcatcattt
     7801 tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat ggattcatcc
     7861 gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc cgacatacag
     7921 gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt gattgaatac
     7981 ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat cttcaagcca
     8041 atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg
     8101 ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag cactagacta
     8161 tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc tgacacatta
     8221 ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg attcactact
     8281 gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt cagctcgatg
     8341 gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa gttctcgtcc
     8401 ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt cgtccgaggt
     8461 agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct tggggaaatg
     8521 acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt
     8581 cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct actcattgat
     8641 aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt tatcgaagaa
     8701 aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa aatcgaatca
     8761 taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat caataaacta
     8821 gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa gatctcattt
     8881 ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca actgatttta
     8941 atgatattct tcagcttacc aatattccca ctaaagggat gagataacat taaagacaag
     9001 gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg aaaaaataat
     9061 tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa cataacacta
     9121 atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc cgtcgtggcg
     9181 aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg acgatagttt
     9241 ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg atggacctca
     9301 tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc cttttcagcc
     9361 cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc gggattgtcc
     9421 gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat ttgctcatgg
     9481 atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt ggctccattt
     9541 attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt ttgaatgacc
     9601 tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat ttgctccgta
     9661 ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc cggtaatttc
     9721 tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg acgagagata
     9781 cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt tagtcgtatc
     9841 agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag tttaatgtgt
     9901 ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta ttccaatttt
     9961 tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat aatttatcta
    10021 ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa atattgaact
    10081 ggattcatat attagtttta ttaatagatt acagaagggt taagtatatt tgcatagttt
    10141 ccaattactt tatccttctg taactttttc aatcttagat ttactagccc gtctcgggcc
    10201 ctattttaca atggttttgt ccacttttct ctggtagaaa aacgtggtag aaaaaataaa
    10261 ataacacaaa aaaagcggcg tttcatccgc ttttggtaga agattggaaa actcagatag
    10321 aaaaaggacg atttttagct ctttagaacg ttgataagtc cccattctta ctgagttttg
    10381 ccattatctc ctatacatct tgaattctaa gaataggtca ttatagatgc ctaggtattt
    10441 cgatcctaga tctttgtaga cttcaagatg ggagatcgtg tcatcagatg gatagaattg
    10501 ttcgtccgac gaaatgtctt ttggcaataa cgcttttgct tcttcatttg gtgtagaata
    10561 accaatatac tctgcatttt gtgccgcatt ttccggttct agcataaagt tgatgaaatc
    10621 ataggcacct tctttatttt tcacggtttt tggcataacg atattatcaa accaaagatt
    10681 ggatccttct ggtgggatca cataatgtaa atgttcattt tcccacatca tatcggcagc
    10741 ttcgccagaa aatgtcactg ccacggaggc ttcttcattg atcatataca ttttgatctc
    10801 atctgccacg attgctttga catttggtgt taaatgattc aatttatttg ctgcttctgt
    10861 caactgtgcc atattcttac tattcaaaga atagcctaaa ctattcaaag aaagccccat
    10921 aacttctcgt gcaccgtcaa tcagcatcaa actgtctttt aattccggct tccacaaatc
    10981 atcccaatgc ttaatttgat ttgcatcaaa gaatttatcg ttatatacga tccctaaagt
    11041 tccccaaaaa tatggaatcg aatactgatt atctggatca aaatccaagt ctaaaaatcg
    11101 tggatcaata ttatttaatc ctttgatctt cgaatgatct aatggaagta ctagtttttc
    11161 tttgatcatt ttttggatca tatattcact tggtatagcg atgtcgtagt ttgtcccacc
    11221 ttgctggatt tttgtgaaca tcgcttcatt agaatcaaat gtttcataat tcactttgta
    11281 tccatattct ttttcaaatt tcgtgatcaa actaggatcg atataatctc cccagttata
    11341 gatggtaagt acctttgctc cactcatgcc acttgttttt tcaagttgac gaacaccaag
    11401 cagcagcacc gcaataatga tgagaatacc taaaaacaga gattgtaatt ttttcatcgc
    11461 agccgtgcca ccccctcgcg tactcttctg tttttcttca gccgttttgg acggttttct
    11521 ttactaataa agtagtatcc aatgaccaat atcatggaga acaagaaaac taacgcactt
    11581 agcgcattga tttctaaact gattccttgt cttgcacgag aatagatctc tactgagagc
    11641 gtcgtaaagc catttcctgt gacgaaaaat gtaaccgcaa aatcatcaag agaataggta
    11701 aatgccataa aatatccagc tatgattcct ggagaaagat aaggcaaaat gatatttttg
    11761 actacttgga aattattagc accaaggtct ctagccgcat cgaccattga atcgttcatt
    11821 tcctgcaatt ttggcaagac catcaagacg acgattggaa tgctgaaagc aatatgggaa
    11881 agcaatacac tctgaaaacc taatccgaat cctagtagtg taaagaaaat caagaaacta
    11941 gcaccgataa taacatcagg agagactaat aagatgttat tcatgctcaa caacgctgtc
    12001 cgtgcacgtc gttttttcgt gtaatagatt cccatagccc caaatgtccc gatgatcgtg
    12061 gcgatcaatg cagataagaa agccagcatg aacgtattca atacgataat gatcaaacgt
    12121 gtatcttcaa aaacagcttc ataattttcc caagtaaatc ctgtaaagct gttcatcgtt
    12181 cctccttcat tgaaggaata aaaaatcaga tagaagatcg gtgcatataa gaataaaaag
    12241 acgatcccta aataaagata cgaccattta aattttttca tttatgagcg acctcctttt
    12301 ttcttctcgc ctgtcaggag catcacgatg aacatagcaa tgattaaaat cacaccgatt
    12361 gtagaaccca tcccccagtt ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa
    12421 gtgatcaccc gatttccgcc aatcagacga gtcagcatga ataaagaaag agaaggaata
    12481 aatactgctt ggacaccact tttcacacca tttaatgata acggaaaaat cactcgtcgg
    12541 aaggtctcaa agctattcgc gcctaaatct ctactagcac tgatcaaaga aggattcatc
    12601 tcttccaatg cattgaaaat tggcataatc ataaatggaa tctcgatata cgttgccacg
    12661 aacagaaaac tgaagtctgt gaataatatc tgtttcggcc cgatcccgat aaaatcaaag
    12721 aaatgattca cgctgccgtg cacactgaaa atcccaataa atgcgtaagc tttaagcagc
    12781 agattcaccc atgtaggcaa aataatcagc atcagccata attgcttgtg cttcagtttt
    12841 gttaaaaaat aagcagtcgg gtaactgatg atcagcgtga aaaacgtgat caaaaatgca
    12901 taccatacag aattgatcgt catagaaagc gatgtccccg acgtaaaata agatagataa
    12961 ttattcagcg tgaattgccc attcatatca aaaaaagatt gataaatgat cattaataca
    13021 ggcgcgatca cgaataaacc gagccacaaa atataaggga cggaatagat cctgcgcatt
    13081 gttttcattc ttgtcccccc tattcttcgt agctgtcaag acgtgcatcg aattcttctt
    13141 cagactcgtt gaaacgcatg acatggatat cttctggctc aaaggccaat cctactttgc
    13201 ttccttcttt agcttttttc gttgaatgga ccatccattc attttgctgt tcatcataac
    13261 agatgatttc ataatggaca ccacgaaaca gctgagtatc gaccgttacg actagttttc
    13321 ctttatctgg tgtcgtgatc gtcaaatcct ctgggcgtaa aacaacttca actggttcat
    13381 ttagacgcat cccgccatcg acacattcaa attgcttacc aacaaactca actagattat
    13441 cttcgatcat tacaccgtcc acgatattgc tctccccaac aaaatcagca acgaaatgat
    13501 tgattggctc atcataaata tctacgggtg taccactttg gacgattttc cctttattca
    13561 tgacaaaaat ctcatcactc attgccaacg cttcttcttg gtcatgagta acaaaaataa
    13621 acgtgattcc taatcgttgt tgcagatccc gcagttcata ctgcatatct gtacgtagtt
    13681 tcaaatcaag cgccgataac ggttcatcta atagcaatac tttcggttca tttacaattg
    13741 ctcgtgcaat cgctacacgc tgtctttgac caccagacat ttcactaatc tcccgttttt
    13801 cgtatccagg aagctggacc atacgcaaag catcttttac cttcttttcg atatctgatt
    13861 tggacatttt cttgatcttc aatccaaaag caacgttatc aaaaacattc atatgaggaa
    13921 aaagtgcata atcttggaag actgtattta cttgtcgctt attagctggc aaatcattca
    13981 accgtttccc ttcaaaatag atgtcccctt ctgtagcttc ggtgaatcca gctataatac
    14041 gtaggatcgt tgttttacca cagccggaag gaccaagcag tgtataaaat tttccttgtt
    14101 cgatctcaaa acttactttt tttaatactg tttcatcatc atagcgtttc actacttgat
    14161 caaatgaaat gattggtttc cccacgtttt taccccctat attacaaata tgattgcgtt
    14221 gcaacaatca atacttttgt tcgttttttg agatgattcc ttaattgatg ttgttttgtc
    14281 gcttgataat agatcgactg tcctgctttt gccacataga ccgtctctcc taagcttaac
    14341 tctaattccc cttctaatac aaaaataaac gtttcagata atgaaggttc aaacgtctta
    14401 tattctcctg caccttcaaa agtcagcaag acaggctcca tttctttttc attcgaatca
    14461 gccacgagcc attccaattc atacccgttt tcttcatcga aataaaccgt atgatcttct
    14521 tttgaataaa ccacttgcag attcgctgct tcttgatgaa aaaattcttc tggagtcaca
    14581 cctaagactt caagaataga aaagaatgtt tccattgatg gtgaacttaa atctcgttcc
    14641 aactgggaaa tatacccttt tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa
    14701 ttcttttgga tgcgcaaatt tcttaacttc tcaccaattt ccataatttc ctccgaatcg
    14761 aagttttcta atagtaaact tcctgtttac taccactcgt attagtatac gaaatttatt
    14821 gaaaaaggca accctttgta aaattttttt tgctgaatat ttctttatct tttggaataa
    14881 attctagttg tttgattctt tatttttcac ctaatttttg tctaaattta cggaagaaca
    14941 agaataaaag agtaggcttt atggtatgat aaaagaaaat tttaaaagga gtgtctcacg
    15001 aatgaacgct tttcgatctt ttcaggtaat aaaggaacct gttttccata cagcaatcag
    15061 tgatctgcca tttactgagt tggctgaaaa tgaagtcttg gtaaaaattg cctattctga
    15121 tgtcaattat aaagatgcat tggcaatgag cgagtctggt caggtgatac gaacatatcc
    15181 gatgactcca gggattgatt tatccggaac tgtcgtccaa agtaacaatc cacgtttttc
    15241 aaaagaagat cttgtacttg ctactggttt tgggcttggc gtcacccatc caggcggtta
    15301 tagccaatat caaaaagtac caggagactg gttagttcct ctaccaaaaa atatgacgct
    15361 gcgtcaagcg atgatccttg gtacagcagg gtttactgct atgttgtgtg tgaacgcttt
    15421 aatccgtcaa ggtatgacct ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt
    15481 aggcagtaca gcgatagccc tgttacataa actaggattc cagtcaatca ttgccttttc
    15541 cagaaaagaa gagtctgtca catggctaaa aagtctaggt gctttgcaag tcgtacgacc
    15601 tgatgaattt ctgccagaga caacaaaagc tttaggaaaa cagcaaatcg actatgtcat
    15661 cgacacagta ggtggcgagc agctaagcag tcttcttcca ttgatctctt ataacggtgc
    15721 agtagcttta tgtggcaatg ctgggggaat caaactaaac gcgactgtct tgccttttat
    15781 tctgcgaaac atccagttga ttggaattga ttctgtcaat gtaccaatag atcaacgtct
    15841 ttctttatgg caacagatgg cggatttgca gattgcagat gaactggtcg tccaagagat
    15901 tactctggat caattgcctg aaacagcatc taaactactt gctggaacac accaaggaag
    15961 aacactagta aatgtaggtg accataaatg aagattctaa tcacagctgg aggaacgact
    16021 gaaaaaatcg atcaagtacg tgctattaca aatcattcga caggacgatt agggcaagca
    16081 ttagctgatt atttagctgc caatccggat accacaatcg attacgtgac tacgaaacaa
    16141 gcactgaaac cgaaaagaca ctccaatata acgatttata cgatcgaatc tgcactggac
    16201 ttatttttac aattagaaga actaacgaaa aaagaacact acgatgcgat tatccacagt
    16261 atggcagtca gtgattttac tcctgctttt agtttttcag aagaacagct ggcaaaaaac
    16321 ctacccactt cttcaacgca agaagaactg gacaattggt ttgcggaaaa tgaacagaca
    16381 gataccactg tttcaaagat ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa
    16441 acaccaaaaa tcatttctta tctacgtgaa tggcagccaa aagcaaaaat cattggtttc
    16501 aaactattag tagatgtacc aaaggaatct cttctggcag tcgcaaaaaa tagtctgata
    16561 aataacaaaa ctgattttat tttcgcaaat gatttgacgg aaatccatgg cgagacacac
    16621 cacggctatc tcttatctaa agatggaaca gtggaagaag ctcaaagtaa atctgagatt
    16681 gctgcattga ttactgagaa aattagactg gaggaatcaa aatgaaaaag atcttactag
    16741 gcgttactgg aagtatttct gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat
    16801 taggtgccca ggtagatgtg attatgtcta aaagcagcac acattttatc acgccattaa
    16861 ccttgcaatc tctgtcgaag cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc
    16921 aggtaatcaa tcatatcgaa ctagcaaaac aagctgactt gtttctcatc gcgcctgcta
    16981 cagcaaacat tatcggcaag ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg
    17041 caatggcgct tccagtggga acaccaaaat tgattgcccc tgccatgaac acgaatatgt
    17101 atcaaaaccc gatcactcag cgtaatctgg aaacattgag atctgtcggc tatcaggaaa
    17161 ttgaaccaag agaatctctt ttagcttgtg gagattttgg aaaaggtgcc ttagctgata
    17221 ttcagacgat catcgaacag gtcatgctga ctcttaatga aagaaatgct tagaaaaaag
    17281 gagtacttat gaaaaataca aaaaacttta cattaactgc gatgttttta gctattttga
    17341 ttttattagc tgttacgcct ttaggtttta tccctatcgg tccaatcaat gcaacaacca
    17401 tgcatattcc tgtaatcatt gcttcgatcg ttttaggacc aagactaggt gcttttcttg
    17461 gcggtacatt tggactgatc agcatgatcc gtagcacagt tatccaaaca cctttgtctt
    17521 ttgtcttttc tccgtttatc ccagtcatcg gaacagaaca tggaagctgg aaagccttgc
    17581 tgatcgcgtt tattcctcgc atcttgattg gggtggttcc ttatttcgtt tataaaggtc
    17641 tgcgcaaact aatgaaacaa aaagcagatt ccgtttcttt attcttagct ggactttccg
    17701 gttcattgac caatacgatt ttagtgatga atatgatcta ctttttattc cagcaagatt
    17761 atgccaaagt catcggaaca aatttaaatg ctgtttattc agctattcta gcggttattt
    17821 ttacaagtgg tatacctgaa ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg
    17881 tcttgttaag atacttcaag cacccttctc ttactaactg atagtcatcg tagccgatct
    17941 ctgatttttt tctcttttgg tgttactatt gaatggaaag atttgacgtc gaaaggatga
    18001 tccaaatgaa aatttttgtt gtaggtgcaa acggacaaat tggccgacac ctgataaaag
    18061 atttagctcc aagttcccat gaaatttttg caggggtgag agatgttgct tcccaaacat
    18121 tagtaaaaaa agagaatgtt tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa
    18181 tgaccgaagc attcaaaggc atcgatgtat tgatttttgc tgccggctct caaggaaaga
    18241 atcttctgca agtagacttg gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc
    18301 atgtctctcg ctatctgatg gtcagtgctg tttatgcaga tgaaccagca aaatggcctg
    18361 agtccatgac agattattat attactaaac attatgcaga tgaatggctg aaacgaacta
    18421 acttagattt tgtcattctt caaccagtca cattgactaa cgatgaagaa gttacttcca
    18481 ttcaattaac taagcctaat gagaaggcat caaaaacgat cactcgctct acagttgctg
    18541 ctgtacttgc cgctctagtg gaagaaacgg atatttctcg tacgactcta gtactttctg
    18601 aaggtagcaa agagttgaat acagcattcc aagaatgggc aaaggaggaa tagatccatg
    18661 cgtgccattt caatcaagca tcctggttct gtagatgtat tagaacaaat tgaagtgcct
    18721 gttcctaaac caaaacaagg cgaattactg atcaaagtcc atacagcagc tgtcaatcga
    18781 acagatatta tgaaaagaga atcagtctct ttgaaacctc cctaccctat tttgggagtc
    18841 gaagtagctg gagaggtagt tgagaataaa agtgatatct ccacttttac tcctgggaca
    18901 aaagtatacg gattagtcaa tctaggtggt tatgccgaat atgctgtgat gcctgccgat
    18961 cgtgcaatcc ttctccctga tacgctagac tatgtttctg ctgctggaat ctcagaagtt
    19021 tttttgactg cttatcaaac actttactgg cttggaaaat tacaagaagg cgaaactgtc
    19081 ttgatccatg caggagcaag tggtgtggga acagcagcga tccaattagc tagacagctt
    19141 tctcacgcga aaatcatcgt tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa
    19201 cttggtgcag atgaattgat caactataaa aaacaagact ttcaagaaga ggtacgaaaa
    19261 atcactgaag gtcggggagt cgatgttatt ttggatttca tcggcgcttc ttactgggaa
    19321 aagaatttag cttctatagc cgttgatgga cgttgggtct tgattggtat gctaggtggg
    19381 accatcgtgc ctgagataga ccttggtgta ttgattggga aacgtgtcca actgcttggt
    19441 acattattga cacctagaag tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa
    19501 aaagctgggc cttttctaga aaatggcaag attcggccaa tcattgaccg tacattccaa
    19561 ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa acaaaaacat cggaaaaatc
    19621 attttgaaga taactgaata aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt
    19681 taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttca
    19741 gcttatttcc gaaggaattt cttctgcttc cgccgtttat acgtgtttag agcgtgagac
    19801 aaaagtgttc tttacttttg tcccacgctc tttttccgaa taaacggtgt tcaaaagctt
    19861 gcatctgcgg taataagtcc aaccaagt
//
LOCUS       DADOOS010000041        19821 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.041, whole genome shotgun sequence.
ACCESSION   DADOOS010000041 DADOOS010000000
VERSION     DADOOS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.041"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            202..1077
                     /locus_tag="J8C37_001252"
     CDS             202..1077
                     /locus_tag="J8C37_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304705.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="J8C37_001253"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="J8C37_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="J8C37_001254"
     CDS             complement(1642..2973)
                     /locus_tag="J8C37_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5304706.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="J8C37_001255"
     CDS             complement(3122..3835)
                     /locus_tag="J8C37_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5304707.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="J8C37_001256"
     CDS             4010..5434
                     /locus_tag="J8C37_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5304708.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="J8C37_001257"
     CDS             5733..6440
                     /locus_tag="J8C37_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAZ5304709.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="J8C37_001258"
     CDS             6427..6750
                     /locus_tag="J8C37_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAZ5304710.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="J8C37_001259"
     CDS             complement(6839..7876)
                     /locus_tag="J8C37_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ5304711.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="J8C37_001260"
     CDS             complement(8197..9180)
                     /locus_tag="J8C37_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAZ5304712.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="J8C37_001261"
     CDS             complement(9595..10365)
                     /locus_tag="J8C37_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304713.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="J8C37_001262"
     CDS             complement(10358..11293)
                     /locus_tag="J8C37_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304714.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="J8C37_001263"
     CDS             complement(11609..13258)
                     /locus_tag="J8C37_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAZ5304715.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="J8C37_001264"
     CDS             complement(13375..15978)
                     /locus_tag="J8C37_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAZ5304716.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="J8C37_001265"
     CDS             complement(15990..17039)
                     /locus_tag="J8C37_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAZ5304717.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="J8C37_001266"
     CDS             complement(17065..17379)
                     /locus_tag="J8C37_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAZ5304718.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="J8C37_001267"
     CDS             complement(17395..17841)
                     /locus_tag="J8C37_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAZ5304719.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="J8C37_001268"
     CDS             complement(17842..19818)
                     /locus_tag="J8C37_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAZ5304720.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         6057 a         3962 c         3222 g         6580 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac a
//
LOCUS       DADOOS010000042        19732 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.042, whole genome shotgun sequence.
ACCESSION   DADOOS010000042 DADOOS010000000
VERSION     DADOOS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.042"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..152
                     /locus_tag="J8C37_001269"
     CDS             <1..152
                     /locus_tag="J8C37_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5304721.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="J8C37_001270"
     CDS             complement(230..763)
                     /locus_tag="J8C37_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAZ5304722.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="J8C37_001271"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="J8C37_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAZ5304723.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="J8C37_001272"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="J8C37_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAZ5304724.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="J8C37_001273"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="J8C37_001273"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAZ5304725.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="J8C37_001274"
     CDS             complement(4488..5066)
                     /locus_tag="J8C37_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAZ5304726.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="J8C37_001275"
     CDS             5388..5858
                     /locus_tag="J8C37_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAZ5304727.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="J8C37_001276"
     CDS             complement(5966..7192)
                     /locus_tag="J8C37_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAZ5304728.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="J8C37_001277"
     CDS             complement(7946..8146)
                     /locus_tag="J8C37_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5304729.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="J8C37_001278"
     CDS             complement(8280..8888)
                     /locus_tag="J8C37_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAZ5304730.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="J8C37_001279"
     CDS             complement(8973..9443)
                     /locus_tag="J8C37_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAZ5304731.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9511..10404)
                     /locus_tag="J8C37_001280"
     CDS             complement(9511..10404)
                     /locus_tag="J8C37_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAZ5304732.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKNLFF"
     gene            10550..11353
                     /locus_tag="J8C37_001281"
     CDS             10550..11353
                     /locus_tag="J8C37_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAZ5304733.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11531..12478
                     /locus_tag="J8C37_001282"
     CDS             11531..12478
                     /locus_tag="J8C37_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAZ5304734.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12546..12839)
                     /locus_tag="J8C37_001283"
     CDS             complement(12546..12839)
                     /locus_tag="J8C37_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAZ5304735.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12867..13532)
                     /locus_tag="J8C37_001284"
     CDS             complement(12867..13532)
                     /locus_tag="J8C37_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAZ5304736.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13672..14304)
                     /locus_tag="J8C37_001285"
     CDS             complement(13672..14304)
                     /locus_tag="J8C37_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304737.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14311..15378)
                     /locus_tag="J8C37_001286"
     CDS             complement(14311..15378)
                     /locus_tag="J8C37_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5304738.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15375..16106)
                     /locus_tag="J8C37_001287"
     CDS             complement(15375..16106)
                     /locus_tag="J8C37_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAZ5304739.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16275..16754)
                     /gene="greA"
                     /locus_tag="J8C37_001288"
     CDS             complement(16275..16754)
                     /gene="greA"
                     /locus_tag="J8C37_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAZ5304740.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16890..18065)
                     /gene="mltG"
                     /locus_tag="J8C37_001289"
     CDS             complement(16890..18065)
                     /gene="mltG"
                     /locus_tag="J8C37_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAZ5304741.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18269..19438
                     /locus_tag="J8C37_001290"
     CDS             18269..19438
                     /locus_tag="J8C37_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAZ5304742.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         5954 a         3961 c         3154 g         6663 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat aaattttttt gatcctttgc
     9541 ctttgtctct gcttggatcg tcacatgtgt aataccatat tttaatttta ataaagaaat
     9601 tatacgagga tatatctttt caatctcgct tagtagctga tcttcaaaat caatatgtcc
     9661 gtcgaaaata attatctttt cgctatactg ccaaatatgg acatgatgaa catcctttac
     9721 cccatcgatt gcttcaattt cttgttccat tttttcatag tcgatagcag gtgcactttg
     9781 tatcaaaata gcggcagctt ttttgatcac cattatcgct tctatcaata tatatatcga
     9841 aatgatcagt gtaataactg gatcgatcca gtagatatcg aaaaaacgga tcaaaattgc
     9901 tccgataatc acacttaccg aagacagtgt atcacttaac aaatgcaaat aactagattt
     9961 tatattcaaa ctttcatctg cttcttgtcg taataataat actgagaaca aattcgcgag
    10021 caatccaata actgctactg ttagcatcaa atgactattt atcttttctg gtgaagtgaa
    10081 ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg atcaatatga atgagttgat
    10141 gaaagcactc aatatttcta aacgctggta gccatacgta cgttttttat ttgcttcttt
    10201 tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc aatgtatcac taaggttatg
    10261 gaaagcatct gaaacaagag acaaactccc tgacataatc cctccaataa attctgataa
    10321 agtgatcacc gcattcaaaa ccactactcc aaatagtttt tttgatgatc tctgtggctg
    10381 tgtaggcact attccttcct ccattttatt tatattggat agtctttaga caagaataac
    10441 atttttttga tatttcacca atcattcagc tgtttcctta aagcacttgt atttcacttg
    10501 atacttcgct agaattgcaa gtgatgacac tactagatga ggagtactta tgacaacaaa
    10561 acaattaatt atcagttcct ttatacgatt caaagaccta aaggaggcga gaaatgtccc
    10621 attttggaaa gccattgtct atcttttagt tttgagtata attatggctt tgccaatctc
    10681 ttttcaagtt tttcaagtct taaacaacat tcaattggat gggcaaaaga ttgctactcg
    10741 cattcctgat tttacaatcc aaaacgggca gcttcaaacc gaagaaaaag aaggattcat
    10801 ttaccaaaca aactccatta tcttcacatt tgatccagag ggaaaacgaa cagaacagga
    10861 catctctact gatttgatgg ggaattttgt tagtgtcggg atgttaaaaa acaaacttat
    10921 catagctctt cctaatacag gcacgacttc tgctttgttg aacaataacc aattagaact
    10981 tccctacacc aatgaatctt taaaaaactt aacaggtaag cagctccgtt cttttttaag
    11041 tgaagcatct ataccacttt ggataaaagt cctgacattt atattttcta tttatcctag
    11101 ttttctgaat ctgctgatca ctttgctatt taccaacatt gctgcttttc tctatgcacg
    11161 ctttcgacta acaaaagcta cttttcttga ttgtttaaaa acattgatct attcaatttc
    11221 attgcctact ataattgcaa caatcttgat ggtctttctt ccttcttttg acactagcgc
    11281 cttcattgct tttgcaggaa tctttatttt tgctcaagct gtcaaaggat gggcaaaaat
    11341 atcgatcaat taagaaataa agacaactcg ttatttctac ttcaatagac ccttttagag
    11401 gttgtgacat ttttttgtca cagccccttt tttatttgaa aaatatttct ttctcaaaaa
    11461 acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca cttagagtaa agaaaggcgg
    11521 gaaattaact atgtcagaaa gaatcattta tgcagatcgt ccatttcgtg atgaatttat
    11581 cgaagaaatc catactatag cgcctggtta tatctttaaa acagaattac aaccagagga
    11641 tctacaaaat gtagagatca gtttgggctg gaactcagac taccaagaaa aactattagc
    11701 ctcttctacc ttgaaatggg tccaatctat ctcagctggt gttgatcagc taccccttga
    11761 gactttttca aagaaacata ttttactttc aaatggtagt ggcatccaca gtgaatcgat
    11821 tacggagcat gtcttaggaa tcatccttgg gtattctcgt ggtctttttc aagcacaaaa
    11881 ggcacagagt gacatgaaat ggttaggttc atcggtccat taccaatcta tccaagggaa
    11941 aaaaatacta atcattggaa ctggccatat tggaaaaatg attgcaaaaa aatgcaaagc
    12001 attagacatg aactgctatg gaatcaatac atccggtcat ccagcagaag gaatggaaaa
    12061 gactttttct ttaaatcaac taaaggaagt tcttcctgaa gtagatttcg tagtcaatat
    12121 cttacctcta acggaggaaa caactgggct ctttgatcag catatgtttg aacactttga
    12181 tccaaaatct gtattcatca atgttggccg tggtgcttct gttaagacac aagatctggt
    12241 gcaggcttta aataaccagc aactttcctt tgcagcatta gacgtttttg aggaagagcc
    12301 tcttccacaa gatcatcctc tttggaaaat ggacaatgta ctgattactt cccacattgc
    12361 aggtttgaca cctgattttc aaaagaaatt aatggctatt tttttaacca atctgaaaag
    12421 ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa ttgtctgcag gatattaata
    12481 gctgaaaaca atattttcaa aagaaaaaag agaaggtgac aaaaattgtg tcaccctctc
    12541 tttttttatt gttttttaaa gtgataggtt ggctcctcca ggttgggatc ataatcaaca
    12601 agtaaatcat atcctttaaa gaaccattca tctgcttcct cgataaaaaa aagtgtccca
    12661 tccatttcca ctttctttac aggatgttct ggctgttcaa ctgacattcc aatcgaaaaa
    12721 ccatcatgaa cctctgtttt gccgtagact tttccaaaaa aacgtattcc gtaagtttct
    12781 ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg gcgttaccgt taatttcatc
    12841 ccaacaacct actttctctt ttctttctat tgattcatct tatcgtctag aatatccaca
    12901 tcgcctgtct ctccaataac cagtagatta tcattttcac gaacatattc atctgctgca
    12961 ggagaagcga taaccgtttg atctactctt cgtatggcga ctactgttaa gtcaaaacgt
    13021 tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt taggattgga gactcggatt
    13081 tctgctaaag aatatttatc tgacaactct aggtaatcta aaatattctt tgacactaaa
    13141 ttatgcgcaa tccttattcc catgtctcgc tcgggatgga cgacgcgatc agctccgatc
    13201 ttttcaagta ctctggcatg atactcattt tgggcttttg ctaaaatatt tggtacgccc
    13261 atttccttta ccatcagagt aactaagata ctggcttgaa tatcttctcc aatagcgaca
    13321 attacatgat cgaaatttct tatgcctaat gatcgaagtg tcatttcatc ctgtgcattt
    13381 gccacaacag catgagtggc tatattcata tattcattga cccgatcttc attactatca
    13441 atagccagta cttcttgacc agcttcgatc agtgttcggc agatacttcc gccaaatcgt
    13501 ccaagaccta caattgcaaa attttgtttc accgttttcc tcctttagta tcttcttttc
    13561 tcagtataac aaagccttta caaaaacaaa agcaagcatc attattgtaa aaaagccaag
    13621 aaaaataata gttattttct cagcttttga ttatcctctt tatcatcgtt tttatttcac
    13681 caaaccatgt ttgaatgcat agatagcggc ttgtgtacgg tcttccactt ctagcttagc
    13741 caatatattg gaaacatgtg tttttactgt tttcagtgta ataaacaatt cgtccgctat
    13801 ttcctgatta ctttttcctt cagaaatcag cattaaaatt tcattttccc gattcgttag
    13861 ttcttcatgt aatacaggct catttcgccg actcattttt tccatcattt ttgttgtcac
    13921 ttctggttct aatactcgct cgccacgttg cgttgccctt atagcatcag caatttcatg
    13981 tgcggtagag gtcttcaaca aataccctgc tgcacctgct tcaatggcag gataaacttt
    14041 ttcgtcatca ataaaactgg ttacgataat gattttagct tctggccaat cttttaagat
    14101 tgcttttgtt gattcgatac catccatttc ttccatgact aagtccatta aaatgacgtc
    14161 cggtcgaagt gtcatcgcct tctcatagcc ttgtcgtcca ttttcagctt ctccaattac
    14221 ttcaatgtct tcttgaatag ataaataaga agagacgcct aagcgcacca tttcatggtc
    14281 atctactaat aaaactttta tcatccttat ctatgcctcc ttcatcaaag ggaccttgat
    14341 ttctacactt gtaccttgac ctttaaaact aatgattttt actgttccac caataccagc
    14401 tactcgttct ttgatattgt tcaatccata acttcccgtt ttcgtttcat tcatattaaa
    14461 ccctgttccg tcgtcaataa tacgtaacaa aagattattg tctattttgt gcaaatatac
    14521 ctctaattca ttggctttag catgtcttaa tgtatttgat aacaattctt gaacgattcg
    14581 gaacaagtga tcctcaatgg aactagatag ttttacatct tctacatccc atttcagcga
    14641 aatctggatt ttattttgca attccttcaa taactgctcg ataccttgct ttaaactttt
    14701 ctcttctaaa ttgactggac gtagatgaag aagcagcgca cgcatctcag actgggaagc
    14761 gttgatgatc tctgctacca ttttcaactg cttgcgaaac atttctggtg tctcgctttt
    14821 ttctgcttgc tccgtcaatg ccgacatcat catcattgct gcaaacaatt gttgcgaaac
    14881 tgaatcatgc agctcacggg ctaatcgatg tctttctaat tctaaaattt cttctttggt
    14941 ttgtccatct acatattggg gacggctcgt tacaatttgt aattcactag atacttcaat
    15001 cattttttct tttatcttag tgatttcctt atcaatatct ttgatgtata aatcgtccga
    15061 tgcacttggg atcggaagaa gcaacaattt ggattcataa ttgccagaag aaagtgcacg
    15121 cagcttttct tcgattttcc cgtattttgt cttttgaata agtgataata atagaaagac
    15181 aattaatcct acacctaagg aaatggctaa caaatggaag ataagcggaa catataataa
    15241 acgtgcacgc agtaattctc cccaccaatg actttgattg ctggcataaa aataagtgaa
    15301 aagtgaaaat agaataataa ggaaagccgc aatccccgaa taaacagcta gcatcgctct
    15361 ggatattttt cccatcatat tcgtatcacc tcgacatctc caatcaaggt attagtaata
    15421 atcttcaatc tacgagggtt ttcatcataa tcactgctgt agattttgat ttgttcattt
    15481 ttcaaagaaa attgctcttc ttcaaacaag acatttccta ctaatgtcgc atgctctaat
    15541 cggatggcta cgcccaaagg tactaagatc cgtgttctgc ctatcccttt tcttacaatc
    15601 acaatattat catcttttgg caaaagcgta ttgcccaaat ctatgatagt atcaccagaa
    15661 atgacggcga tgttgatatc gtcccattca tagacgtcgt ttccaatacg ttggttaccg
    15721 aataattgct gtcttttccg ctcattatta tgtgttttta attgctctgt ttggatcatc
    15781 atgatctgtt tctttcgcca aaaagcattt ttggttaaat ctatccctga aatctctaca
    15841 cctttcagac caataaataa aacggcaaat acagccatca tccataaagc aggactgttg
    15901 actaagctaa aaaaaatgac caaacttcct aatatcaatt gaaaattttg aaatttagta
    15961 cgaggatgtt tgcgcatagc tagatatata ttgaaaatac cgaatatcac taaaagcaaa
    16021 agtccagtat tgtttacgat ttgccaaaca gcaaataaca gcagtagtgc ttcaactacc
    16081 acaaaaaaac gccaagaact attcatcgct ctttctccct cctgacacat tctcatttca
    16141 tcatacctat ttttttaact gttggctata ggactttaga tgtaagatcg tttgttgaaa
    16201 tctaaaatac acaaagagaa aaagatctag agtgtatcat cagtaagatg aactctctag
    16261 atctttcagt cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct
    16321 ggagtagcaa tagttacttc atcattttta cgtttgccaa tcaatgcttg cgcaatagga
    16381 gaatcattgg aaattttgcc agaaaacgga tctgcttcag cgcttccgac aatcgtgtac
    16441 tcttcttctt ctccatctgg tagttcgatg aatgtaactg ttttaccaat tgatacctca
    16501 tctgcatcta caccgtcgtt atcgatgatt tgagcaaaac gaatcatatt ttcaattgtt
    16561 gtgattcttc cttcaacaaa tgcttgttca tctttcgctg attcatattc agagttttcg
    16621 gaaagatctc cgaaaccgcg ggcaattttg attcgttcaa tgatttctcc gcgtttgact
    16681 gtttttagtt cttctaattc tttttctaat ttttctttac cttctagagt cattggataa
    16741 actttctcta ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt
    16801 ttgaggaata tagtccaaaa aagattacca cgcttttctt caaatgtaaa tgtttttcta
    16861 aaaatgatca atgtctagag cctgtttttc taagaattat ttacatattt ttccactaat
    16921 tccatatgtt cttcataagt tttagcgaaa taaacatttc cagtgctcgt atctgctacg
    16981 aaataataat agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga
    17041 ttgtcaaaag gtccaggtcc atagccggta ttggtataca aattatatgg tgaatctaca
    17101 gctgtatctt catacgtcac taattctttg tgttcaccta atgcgtataa aacagagata
    17161 tctgactgaa gtggcatatt tgctttgatt cggttaaaga agacttgtgc aatgttttta
    17221 cgatcatttt cttttacgcc ttctttttca actagagaag ctagagttaa aacttcttgt
    17281 acagtcaaat ttttctgctt gatagctgaa taatagtttt gcatgaccgt attcgtcttg
    17341 ttcaccattt caatcactag atccttaagc gaagtactcg tgtaataatc atatgtggct
    17401 gggaataagt aaccttccaa acggtatttt gtgttctcag ctttagatgc actttccaac
    17461 agttctggga atttttggtg caactcgtta aagaaagtct catcattcac taaatctaaa
    17521 aattctttct tgtctttacc agttaccttc gctacccgtt cagcaatctg atcgatatcg
    17581 tacccttctg gaatggcaat tttcccatct gcaacttttg ttggctcaga agtaccgcct
    17641 tcttgcaact gtttgccaat ctcgtcaaga gtcatattag gcgctaactg ataatagcca
    17701 gcttgaaagc cagttaaatt tttaaatttt gtataataat tgaaaacaat cccgctttta
    17761 ataatattgt ccttctccaa aatctcacca atctgcttgt tagaagagcc actcgggatc
    17821 tcgacttgga ctaattggtc atctgattta tctaaaggct tcaacccgct atctacataa
    17881 cgatacacgg taaatccaag aaatccgcca ataatcaata atgtaagagc aatcactaaa
    17941 atgatcttcc tcacaatctt atcttctttt ttacgcagat ttctccgctt gtcgattata
    18001 tctgctgtag ctgccttact ggcttctttt ttatctttac ttgggtgttg tttgtttcct
    18061 gacaaaacaa ctcctcctta ctatcaagtt cgtaacttta ttcattatac atgatatttg
    18121 aattggcgtg aatgatttta tttaaatatt ctatcaaaaa tgtaagtagt aaatccatta
    18181 ctataagatt aaaataaatg ttatcaagat acaagctatt ttagctttat tctttctgta
    18241 aaaaagaaag gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa
    18301 atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac
    18361 tattcttgga tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc
    18421 agctcaaaaa aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca
    18481 actattaaga aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat
    18541 catggaaaaa ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct
    18601 ttattatcag attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt
    18661 cgagcgtggg cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg
    18721 cagccagcaa attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc
    18781 ttctccaacc ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa
    18841 atttacagat tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat
    18901 tttgatcaaa ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc
    18961 tttaggatta cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact
    19021 atcctatcaa atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga
    19081 gatccataac gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct
    19141 gtttacacaa gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg
    19201 tgaaaatgcg aatggacatc catatagtgg tggggtatat agcggagata attatatcct
    19261 gtcgcaagga aatatcgact cttttgatac atctgctata tttagcaaag attcacagga
    19321 tgctatcatc atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga
    19381 tctaagaaac caaatctatg acctgatgga aggaactact aagttggcaa atagctgata
    19441 tgatgaaaag atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga
    19501 ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat
    19561 tccaaataaa cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag
    19621 atttctcttt accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag
    19681 attgctaaaa tatgtgctag caaatcattc atggcttgtt cttattttta at
//
LOCUS       DADOOS010000043        19457 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.043, whole genome shotgun sequence.
ACCESSION   DADOOS010000043 DADOOS010000000
VERSION     DADOOS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.043"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            133..3660
                     /locus_tag="J8C37_001291"
     CDS             133..3660
                     /locus_tag="J8C37_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ5304743.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="J8C37_001292"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="J8C37_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAZ5304744.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="J8C37_001293"
     CDS             7448..7789
                     /locus_tag="J8C37_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304745.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="J8C37_001294"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="J8C37_001294"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAZ5304746.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="J8C37_001295"
     CDS             9146..11566
                     /locus_tag="J8C37_001295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAZ5304747.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="J8C37_001296"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="J8C37_001296"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAZ5304748.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="J8C37_001297"
     CDS             13000..13737
                     /locus_tag="J8C37_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304749.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="J8C37_001298"
     CDS             13751..14566
                     /locus_tag="J8C37_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAZ5304750.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="J8C37_001299"
     CDS             14579..15226
                     /locus_tag="J8C37_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ5304751.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="J8C37_001300"
     CDS             15242..15898
                     /locus_tag="J8C37_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ5304752.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="J8C37_001301"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="J8C37_001301"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAZ5304753.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="J8C37_001302"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="J8C37_001302"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAZ5304754.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="J8C37_001303"
     CDS             18240..18758
                     /locus_tag="J8C37_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAZ5304755.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="J8C37_001304"
     CDS             18865..19365
                     /locus_tag="J8C37_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ5304756.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DADOOS010000044        19271 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.044, whole genome shotgun sequence.
ACCESSION   DADOOS010000044 DADOOS010000000
VERSION     DADOOS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.044"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            173..2644
                     /locus_tag="J8C37_001305"
     CDS             173..2644
                     /locus_tag="J8C37_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ5304757.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2747..4483)
                     /locus_tag="J8C37_001306"
     CDS             complement(2747..4483)
                     /locus_tag="J8C37_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAZ5304758.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4480..5184)
                     /locus_tag="J8C37_001307"
     CDS             complement(4480..5184)
                     /locus_tag="J8C37_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304759.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5315..5818
                     /locus_tag="J8C37_001308"
     CDS             5315..5818
                     /locus_tag="J8C37_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAZ5304760.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5778..6116
                     /locus_tag="J8C37_001309"
     CDS             5778..6116
                     /locus_tag="J8C37_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAZ5304761.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6137..7555
                     /gene="ffh"
                     /locus_tag="J8C37_001310"
     CDS             6137..7555
                     /gene="ffh"
                     /locus_tag="J8C37_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAZ5304762.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7667..8245
                     /locus_tag="J8C37_001311"
     CDS             7667..8245
                     /locus_tag="J8C37_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAZ5304763.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8249..8770
                     /locus_tag="J8C37_001312"
     CDS             8249..8770
                     /locus_tag="J8C37_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ5304764.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8849..9403)
                     /locus_tag="J8C37_001313"
     CDS             complement(8849..9403)
                     /locus_tag="J8C37_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ5304765.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9656..9931
                     /gene="rpsP"
                     /locus_tag="J8C37_001314"
     CDS             9656..9931
                     /gene="rpsP"
                     /locus_tag="J8C37_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAZ5304766.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9943..10194
                     /locus_tag="J8C37_001315"
     CDS             9943..10194
                     /locus_tag="J8C37_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAZ5304767.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10319..11110
                     /locus_tag="J8C37_001316"
     CDS             10319..11110
                     /locus_tag="J8C37_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304768.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11280..11801
                     /gene="rimM"
                     /locus_tag="J8C37_001317"
     CDS             11280..11801
                     /gene="rimM"
                     /locus_tag="J8C37_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAZ5304769.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11791..12552
                     /gene="trmD"
                     /locus_tag="J8C37_001318"
     CDS             11791..12552
                     /gene="trmD"
                     /locus_tag="J8C37_001318"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAZ5304770.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12688..13035
                     /gene="rplS"
                     /locus_tag="J8C37_001319"
     CDS             12688..13035
                     /gene="rplS"
                     /locus_tag="J8C37_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAZ5304771.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13167..14303)
                     /locus_tag="J8C37_001320"
     CDS             complement(13167..14303)
                     /locus_tag="J8C37_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5304772.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14419..15333)
                     /locus_tag="J8C37_001321"
     CDS             complement(14419..15333)
                     /locus_tag="J8C37_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAZ5304773.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15424..15852)
                     /locus_tag="J8C37_001322"
     CDS             complement(15424..15852)
                     /locus_tag="J8C37_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAZ5304774.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15857..16231)
                     /locus_tag="J8C37_001323"
     CDS             complement(15857..16231)
                     /locus_tag="J8C37_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304775.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16517..16702
                     /locus_tag="J8C37_001324"
     CDS             16517..16702
                     /locus_tag="J8C37_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304776.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16714..16962
                     /locus_tag="J8C37_001325"
     CDS             16714..16962
                     /locus_tag="J8C37_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304777.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            16993..17172
                     /locus_tag="J8C37_001326"
     CDS             16993..17172
                     /locus_tag="J8C37_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304778.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17215..17685)
                     /locus_tag="J8C37_001327"
     CDS             complement(17215..17685)
                     /locus_tag="J8C37_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304779.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17772..18470
                     /locus_tag="J8C37_001328"
     CDS             17772..18470
                     /locus_tag="J8C37_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAZ5304780.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18648..18989
                     /locus_tag="J8C37_001329"
     CDS             18648..18989
                     /locus_tag="J8C37_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304781.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            18982..>19271
                     /locus_tag="J8C37_001330"
     CDS             18982..>19271
                     /locus_tag="J8C37_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAZ5304782.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6344 a         3372 c         3792 g         5763 t
ORIGIN      
        1 cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg ctatatgaca
       61 ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg ttctatactg
      121 gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa
      181 taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag ataaagtcat
      241 ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg aacgaataga
      301 aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt
      361 ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt tgctttgttt
      421 agtagaatcc tataagaact tgactttttt accagttatc atagccaata caggaatcgc
      481 catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt taaatacagc
      541 aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag accttgtaat
      601 agatgacatt gttattttag agactggata tcaaattcca gcagatgcag aggtcgtgga
      661 agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa taaccaaaga
      721 aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt acgcccgatt
      781 agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca agacaatggg
      841 agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat ggattgggat
      901 catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta atggaaacac
      961 gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac ctgaaggact
     1021 gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa agcaaagagt
     1081 tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc tttgtgtaga
     1141 taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa
     1201 acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact atgcaaaagc
     1261 gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa aaaccactga
     1321 tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt
     1381 ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga
     1441 atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt
     1501 gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat taggctatat
     1561 tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact tcaaaaaaca
     1621 gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca atgtagctgt
     1681 ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat cagaagaaga
     1741 atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag aacaaaagaa
     1801 aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg atggcgtgaa
     1861 tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc
     1921 cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt
     1981 tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt
     2041 gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc
     2101 tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc catctttctt
     2161 gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga
     2221 aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg
     2281 ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc
     2341 agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat
     2401 cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc
     2461 aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga
     2521 ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa
     2581 agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta aacgagaaaa
     2641 ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag
     2701 caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa
     2761 tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccattaa
     2821 ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgtcggc
     2881 ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga
     2941 tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa
     3001 taatctcgct gtcgcttttt gaatattgga cagcattttc aattagattt ttgataattg
     3061 gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca
     3121 accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg
     3181 tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc
     3241 gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag
     3301 tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca
     3361 caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac
     3421 gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag
     3481 taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat
     3541 ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa
     3601 tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct
     3661 ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca
     3721 gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga tacatcttac
     3781 tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga tcgggtgttt
     3841 gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg
     3901 ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga
     3961 agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac
     4021 cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta
     4081 atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg ctacctaggt
     4141 tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt
     4201 cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg
     4261 actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat
     4321 ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga
     4381 tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa
     4441 gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa
     4501 cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat
     4561 tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa
     4621 attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa
     4681 ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac
     4741 ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc
     4801 atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac
     4861 ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat
     4921 ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg
     4981 acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat
     5041 gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt
     5101 taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc
     5161 atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata
     5221 ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga
     5281 aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg
     5341 cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt
     5401 tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag
     5461 agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt
     5521 atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta
     5581 tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg
     5641 cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg gtgccggagt
     5701 ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa
     5761 aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat
     5821 tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg
     5881 atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca
     5941 atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat cttttttcta
     6001 attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc
     6061 cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa
     6121 aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg
     6181 aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat
     6241 ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg
     6301 cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat
     6361 cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa
     6421 taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac
     6481 gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct
     6541 aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca
     6601 gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca
     6661 agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg
     6721 tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc
     6781 aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga
     6841 tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac
     6901 tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg
     6961 ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat
     7021 ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact acgacgagaa
     7081 aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat
     7141 tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc
     7201 aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg
     7261 caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa
     7321 cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg
     7381 aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat
     7441 gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat
     7501 ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga
     7561 aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt
     7621 tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg
     7681 gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata
     7741 tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg
     7801 gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa
     7861 aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat
     7921 tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca
     7981 agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga
     8041 ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc
     8101 aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt
     8161 agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt
     8221 acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag
     8281 tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac
     8341 aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag
     8401 aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa
     8461 gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga
     8521 agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt
     8581 gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat
     8641 ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga
     8701 agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt
     8761 acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata
     8821 gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat
     8881 acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat
     8941 caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca
     9001 aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg
     9061 atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc
     9121 ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga
     9181 aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct
     9241 gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac
     9301 tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat
     9361 cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc
     9421 aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa acacttactg
     9481 acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc
     9541 tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa
     9601 aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc
     9661 agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt
     9721 agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc
     9781 tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa
     9841 aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa
     9901 acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac
     9961 ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc
    10021 gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt
    10081 atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg
    10141 atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac
    10201 gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt gaagaaatga
    10261 ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat
    10321 gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac
    10381 tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga
    10441 aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct caaataatga
    10501 gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga
    10561 tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc
    10621 tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga
    10681 aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca
    10741 agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat
    10801 cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg
    10861 gcgtattcga atccgtggga ataatataga aggccaagat cccttactat tagctaaaga
    10921 aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac
    10981 tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa
    11041 tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa
    11101 gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt
    11161 aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact
    11221 agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt
    11281 tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc
    11341 gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgctaacac
    11401 tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa
    11461 attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg
    11521 atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt
    11581 accatgagat catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag
    11641 aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata
    11701 tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc
    11761 gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca
    11821 agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta
    11881 ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat
    11941 gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac
    12001 atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca
    12061 gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta
    12121 ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa
    12181 gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat
    12241 gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct
    12301 tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg
    12361 aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa
    12421 gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca
    12481 cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat
    12541 gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat gttagtattt
    12601 tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa agctcataag
    12661 aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa
    12721 caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa
    12781 gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc
    12841 ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa
    12901 cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa
    12961 gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa
    13021 gaaatccgtc gttaatcatc gttatcagac tgagaaagaa cccttggtat tcaagggttc
    13081 tttttttgtt ctctgttttt ttatattaac gatttctatt tgggacattt ttgattaata
    13141 aaaaggggca aaaaaggggc aacaagttac agtttttcta tatcttcttt caatttttct
    13201 tttactccct ttgtcacatg caaataaatc ttttcagtga tatcactatt ttcgtgccca
    13261 acgcgatcct gaatagcata cagaggagtt cctagttccg ctaattttga aatatgggta
    13321 tgtctaaaaa tatgtgaact aagtttttta tcaatcttca tgtcagcttt atgatttctc
    13381 aaataggtgt taattgctgt tagttggaaa ggagttcctt tcgttgtttg aaatagaaat
    13441 tgaccattcg gatttaattc tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa
    13501 tcaatttctc gcattccagc agcagttttt gtagaatcag attttttcat atcagctatt
    13561 gaacgttctc tatacatcat cgtcccattt ataactactg atgcattatt attggtaatg
    13621 tgtacatcgt ctttacttaa tgcgattgct tctccaggcc tcattccagt caaatatagc
    13681 cactggaaaa ggagagaata tcttttattg tgtgaagttg tgaaatttac tagtctttta
    13741 tattcatcat cttctaaaaa cttatcttta atttttatcg tttttgactc tcgtttataa
    13801 tcaataacta cttcgttgat aggatttttt tctacatagc cttttttcat agcgtatgaa
    13861 aaaagaagat tcaatttgga tttaattaca ctgacgtact tatttgacaa gtcatcttta
    13921 tatatcatat cttcaaaaat attatttaaa tctactgtat taataccaga aacgatgtaa
    13981 gtctcaggta tctttttctt tatagtgttt aagatattat tcgtaggata atatgtagac
    14041 tccttaactt gtcttttgta aataactaac cattcttcga caagctcatg aaatgttaga
    14101 tcaggcttct gaagtgtttt ttcgtttaat tttatatcaa tttttttatt taactctagc
    14161 aatgccactt tctgagtttc tcgagattta tttttataag taatgctcac tttttttctt
    14221 tttctagttt ttggatcaat atatcgctca ttatatttat atactttttt tccgttttta
    14281 tcagttttgg tttcaatcca cattgttttc atctcctatt tgttgctata ataggaatgg
    14341 ataagtaagc caattatagc aggtttattt ttcatcacgt ccacaaactt tggcgaggga
    14401 gggggcgtgt ttttttgttt agtagctacc tttccatgaa gtatctgcga agaacacttc
    14461 aaatgttgtt ccatcatttt tcacttcaaa atatgctgtt ccttgtgcgg atttacccgg
    14521 ttggatggtt tccgaaaagt aatctctact agctaaatca gatttaatat ctgatgaatc
    14581 ataaaattct aaataacttg aacttgcatc aaaaggtgta gtccctgtgt tttctacaag
    14641 aaaatctacc tttgcgtaaa ataggccttc tggtttatag aaatcatcac cgtaatcttt
    14701 tgatacagac tggacagtta cgttaataga gttaccattt tcagaagtaa aaggaactgc
    14761 ttctcctata ctatgtgcaa ctgatgccgt ctggggttct gaagacgaag ttgtagttgt
    14821 agttgtagta gagcttgaat ctttggattt tccatcttta tctaagaaag ttcgttgatt
    14881 ataatataca acctgttcag tttttggact aacccaaatt aacattgttt catcatcaga
    14941 atcagttgaa taagtatatt ggagcatctc taattttttt atatcttttg cactatttcc
    15001 atgttcaatt aattcagagt gctctttata ataatctcct tgtgcatcct ttgaaataga
    15061 tacaattaat tcatcccgtg tgatagcatt gtctaattct ttgtaaacaa aatcatttgt
    15121 ccatttacgt aacggtgaac caagtgtctg ataaacttga gttaaatcat caccaacttt
    15181 tattttatga acgtctgatg aagttagttt ttttgtttca tattcagatg ttgatgaaac
    15241 agtattttct ttagtgctag cagaattagg actagtggag catccgctta ataccaagcc
    15301 taaaaaaagc aacccaaaaa caattttttt catttttaat tcctcacttc tatgatatga
    15361 tttttttgta ggatcttaga aataaggttt ttagtccgtg ttgcagcacg gacttttttt
    15421 acattatata agaaagcgat aagcgctttc tggaagtccg taaagattct ttaattcctc
    15481 aattcgttta ggatattgat cattgtcttc tttgtataga gaaacaatga gattagcagc
    15541 aaagcaatta gcttcgcttt cagatttgct tctagatgtt cttgttgata catagtaact
    15601 ggataagcca cgatgaaaaa tagcgtgacc taattcgtga gcgcaaatgt agaatctttc
    15661 ctcagagtct ctcagttcat catttaagaa gattattgca cgacctctaa tttcttgaaa
    15721 ctgtcctttg ggattctcga taaaaggaac gtattgaatt ttaatgccca tcttttcaca
    15781 aatataaaaa ggattagcgg actggtattt ccgcttcaac tcctcgacta aattaatcgt
    15841 atccatctcc ataagctcac atctttttgc ctttttcttt gtcttctttc acaatatccc
    15901 agaaggttcc aataaggata tcttttacgc gttggacctg ttcaggcgtc agtgtttctc
    15961 cgccataaga catattgaca tttgaatcta gtaatttatc aagttcaact acttcatctt
    16021 ttgttgccca gtcaggaact tggtttctac cgagtaaata atcagtagtt acattaaaat
    16081 aatctgctac aagttgtagt cttttagtac taggagttct atttttccat tgataaatcg
    16141 tgtttttagg tatatttaat tcttcttcca attgagttat acttatgcct cttttatgag
    16201 ctaattcctt tattctatct agtaaattca ttttcgcacc tcacaagcta cgaaaacact
    16261 aataaaaaaa ttagttaaag gtgttgacaa ctaataaaag tgttagtata ctgttttcgt
    16321 aagctaaaat attagctaaa aagacttgat aactaataaa cacttcacgg tcggcaaact
    16381 ctgaaatgta aattactagg catttctgtg tcttatttag ctatgtctat atactaataa
    16441 aaatattagt tattgtcaac gaatattagc taatttttta gcttacagat tattttttta
    16501 gaaaggagct atttttatgt ctgagaattt agacttaaaa attcgagcgg agatgagaaa
    16561 aagaagaatg actttcaaag aactagctgc gcttatcggt atttcaggag cttatttatc
    16621 agatattcta aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa
    16681 aaaaatattg gacatccgat aggggggtag gcgatgacaa aactaaaaaa acaagatttt
    16741 gtaaaaaaat acaattattc tccatctact tatcaacgtc gaatgtcgga actaaaaaat
    16801 acagcaattt tctcagcggc gtatgaacgg gtcacaggac aagaagtttg gatcaataca
    16861 gaattatacg ataaattttt gtctttcaaa tcctataaca ggttacgcac aagaaaggta
    16921 acacctaaag aatttatcga gaagcattta gttgatttat aaaaaacaga acattttgag
    16981 aggtgaaggt taatgggtaa attcaacaga gcattggtat tcagcgcacc gctaatcatc
    17041 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
    17101 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
    17161 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
    17221 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
    17281 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
    17341 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
    17401 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
    17461 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
    17521 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
    17581 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
    17641 tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt
    17701 taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga
    17761 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct
    17821 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
    17881 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
    17941 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
    18001 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
    18061 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
    18121 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
    18181 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
    18241 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
    18301 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
    18361 tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa
    18421 ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg
    18481 ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta
    18541 agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg
    18601 caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac
    18661 tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa
    18721 caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag
    18781 atgatttagg caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc
    18841 attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag
    18901 agtacgacag agataacttg attgatatct cttattacgg accaggtaag aaatacattg
    18961 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
    19021 tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg
    19081 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
    19141 tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
    19201 ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac
    19261 acggaaaccg a
//
LOCUS       DADOOS010000045        19130 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.045, whole genome shotgun sequence.
ACCESSION   DADOOS010000045 DADOOS010000000
VERSION     DADOOS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.045"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..703)
                     /locus_tag="J8C37_001331"
     CDS             complement(<1..703)
                     /locus_tag="J8C37_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304783.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="J8C37_001332"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="J8C37_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="J8C37_001333"
     CDS             1758..2837
                     /locus_tag="J8C37_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ5304784.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="J8C37_001334"
     CDS             2869..3462
                     /locus_tag="J8C37_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304785.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="J8C37_001335"
     CDS             complement(3572..3799)
                     /locus_tag="J8C37_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5304786.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="J8C37_001336"
     CDS             complement(3902..6490)
                     /locus_tag="J8C37_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAZ5304787.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="J8C37_001337"
     CDS             complement(6510..7169)
                     /locus_tag="J8C37_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ5304788.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="J8C37_001338"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="J8C37_001338"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAZ5304789.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="J8C37_001339"
     CDS             complement(7942..8808)
                     /locus_tag="J8C37_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ5304790.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="J8C37_001340"
     CDS             8917..9558
                     /locus_tag="J8C37_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAZ5304791.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="J8C37_001341"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="J8C37_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAZ5304792.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="J8C37_001342"
     CDS             complement(10999..11898)
                     /locus_tag="J8C37_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAZ5304793.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="J8C37_001343"
     CDS             complement(11900..12697)
                     /locus_tag="J8C37_001343"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAZ5304794.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="J8C37_001344"
     CDS             complement(12675..13379)
                     /locus_tag="J8C37_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAZ5304795.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="J8C37_001345"
     CDS             13510..14085
                     /locus_tag="J8C37_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAZ5304796.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="J8C37_001346"
     CDS             14203..14880
                     /locus_tag="J8C37_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAZ5304797.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="J8C37_001347"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="J8C37_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAZ5304798.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="J8C37_001348"
     CDS             complement(16943..17941)
                     /locus_tag="J8C37_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAZ5304799.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="J8C37_001349"
     CDS             complement(18167..18820)
                     /locus_tag="J8C37_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAZ5304800.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVIDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5526 a         4136 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcaatc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DADOOS010000046        18989 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.046, whole genome shotgun sequence.
ACCESSION   DADOOS010000046 DADOOS010000000
VERSION     DADOOS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.046"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            13..201
                     /locus_tag="J8C37_001350"
     CDS             13..201
                     /locus_tag="J8C37_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5304801.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(259..612)
                     /locus_tag="J8C37_001351"
     CDS             complement(259..612)
                     /locus_tag="J8C37_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304802.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1143..1565
                     /locus_tag="J8C37_001352"
     CDS             1143..1565
                     /locus_tag="J8C37_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ5304803.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1562..1792
                     /locus_tag="J8C37_001353"
     CDS             1562..1792
                     /locus_tag="J8C37_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304804.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2290..2490
                     /locus_tag="J8C37_001354"
     CDS             2290..2490
                     /locus_tag="J8C37_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ5304805.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2517..3710
                     /locus_tag="J8C37_001355"
     CDS             2517..3710
                     /locus_tag="J8C37_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5304806.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3778..5343)
                     /gene="guaA"
                     /locus_tag="J8C37_001356"
     CDS             complement(3778..5343)
                     /gene="guaA"
                     /locus_tag="J8C37_001356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAZ5304807.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5402..6325)
                     /locus_tag="J8C37_001357"
     CDS             complement(5402..6325)
                     /locus_tag="J8C37_001357"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAZ5304808.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6521..7126
                     /locus_tag="J8C37_001358"
     CDS             6521..7126
                     /locus_tag="J8C37_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ5304809.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7434..8450
                     /locus_tag="J8C37_001359"
     CDS             7434..8450
                     /locus_tag="J8C37_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5304810.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8591..9892
                     /locus_tag="J8C37_001360"
     CDS             8591..9892
                     /locus_tag="J8C37_001360"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAZ5304811.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9938..10600
                     /gene="deoC"
                     /locus_tag="J8C37_001361"
     CDS             9938..10600
                     /gene="deoC"
                     /locus_tag="J8C37_001361"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAZ5304812.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10621..11019
                     /locus_tag="J8C37_001362"
     CDS             10621..11019
                     /locus_tag="J8C37_001362"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAZ5304813.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11085..12167
                     /locus_tag="J8C37_001363"
     CDS             11085..12167
                     /locus_tag="J8C37_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5304814.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12379..13941
                     /locus_tag="J8C37_001364"
     CDS             12379..13941
                     /locus_tag="J8C37_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304815.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13934..15067
                     /locus_tag="J8C37_001365"
     CDS             13934..15067
                     /locus_tag="J8C37_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304816.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15067..16023
                     /locus_tag="J8C37_001366"
     CDS             15067..16023
                     /locus_tag="J8C37_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304817.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16122..17288
                     /gene="deoB"
                     /locus_tag="J8C37_001367"
     CDS             16122..17288
                     /gene="deoB"
                     /locus_tag="J8C37_001367"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAZ5304818.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17312..18130
                     /locus_tag="J8C37_001368"
     CDS             17312..18130
                     /locus_tag="J8C37_001368"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAZ5304819.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18147..18854
                     /gene="deoD"
                     /locus_tag="J8C37_001369"
     CDS             18147..18854
                     /gene="deoD"
                     /locus_tag="J8C37_001369"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAZ5304820.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6064 a         3441 c         4100 g         5384 t
ORIGIN      
        1 gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg aaataaaaca
       61 agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg ccccaaatgt
      121 aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa agagccagac
      181 gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg ctctttttgt
      241 ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac cttttgctag
      301 tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt ctacctgtaa
      361 ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa tagatacatg
      421 gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt tgttttctat
      481 atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac gagtgagacc
      541 ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt atttatcttc
      601 ttttttacgc atactaaatc acctctatac attttactgt tcctatataa ccataaacag
      661 gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg aatttattaa
      721 tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat acaactttat
      781 aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc
      841 gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt
      901 atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa
      961 aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata
     1021 aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta
     1081 gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa
     1141 gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
     1201 aagttattga agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag
     1261 aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt
     1321 acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt
     1381 tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt
     1441 actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg
     1501 tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag
     1561 aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga
     1621 cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt
     1681 acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg
     1741 cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt
     1801 tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca
     1861 tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac
     1921 aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc
     1981 atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga
     2041 ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt
     2101 tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg
     2161 tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat
     2221 aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa
     2281 ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct
     2341 ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac
     2401 tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt
     2461 cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg
     2521 ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt
     2581 atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag
     2641 caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg
     2701 ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg
     2761 taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg
     2821 ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat
     2881 caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg
     2941 atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga
     3001 aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc
     3061 taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta
     3121 tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact
     3181 caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg
     3241 ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt
     3301 atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct
     3361 ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc
     3421 tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa
     3481 tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga
     3541 cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat
     3601 taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc
     3661 aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aagtgcagta
     3721 aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct cctacaacta
     3781 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     3841 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     3901 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     3961 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     4021 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     4081 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     4141 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     4201 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     4261 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     4321 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     4381 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     4441 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat cgactttgat
     4501 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     4561 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     4621 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     4681 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     4741 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     4801 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     4861 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     4921 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     4981 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     5041 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     5101 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     5161 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     5221 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     5281 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     5341 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     5401 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     5461 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
     5521 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
     5581 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
     5641 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
     5701 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
     5761 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
     5821 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
     5881 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
     5941 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
     6001 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
     6061 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
     6121 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
     6181 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
     6241 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
     6301 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
     6361 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
     6421 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
     6481 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
     6541 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
     6601 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
     6661 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
     6721 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
     6781 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
     6841 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
     6901 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
     6961 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
     7021 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
     7081 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
     7141 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
     7201 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
     7261 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
     7321 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
     7381 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
     7441 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
     7501 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
     7561 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
     7621 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
     7681 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
     7741 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
     7801 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
     7861 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
     7921 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
     7981 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
     8041 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
     8101 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
     8161 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
     8221 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
     8281 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
     8341 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
     8401 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
     8461 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
     8521 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
     8581 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
     8641 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
     8701 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
     8761 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
     8821 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
     8881 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
     8941 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
     9001 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
     9061 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
     9121 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
     9181 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
     9241 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
     9301 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
     9361 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
     9421 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
     9481 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
     9541 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
     9601 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
     9661 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
     9721 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
     9781 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
     9841 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
     9901 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
     9961 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    10021 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    10081 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    10141 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    10201 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    10261 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    10321 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    10381 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    10441 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    10501 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    10561 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    10621 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    10681 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    10741 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    10801 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    10861 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    10921 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    10981 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    11041 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    11101 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    11161 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    11221 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    11281 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    11341 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    11401 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    11461 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    11521 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    11581 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    11641 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    11701 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    11761 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    11821 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    11881 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    11941 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    12001 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    12061 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    12121 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    12181 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    12241 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    12301 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    12361 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    12421 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    12481 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    12541 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    12601 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    12661 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    12721 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    12781 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    12841 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    12901 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    12961 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    13021 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    13081 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    13141 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    13201 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    13261 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    13321 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    13381 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    13441 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    13501 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    13561 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    13621 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    13681 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    13741 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    13801 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    13861 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    13921 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    13981 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    14041 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    14101 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    14161 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    14221 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    14281 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    14341 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    14401 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    14461 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    14521 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    14581 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    14641 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    14701 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    14761 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    14821 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    14881 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    14941 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    15001 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    15061 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    15121 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    15181 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    15241 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    15301 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    15361 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    15421 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    15481 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    15541 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    15601 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    15661 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    15721 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    15781 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    15841 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    15901 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    15961 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    16021 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    16081 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    16141 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    16201 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    16261 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    16321 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    16381 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    16441 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    16501 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    16561 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    16621 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    16681 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    16741 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    16801 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    16861 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    16921 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    16981 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    17041 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    17101 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    17161 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    17221 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    17281 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    17341 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    17401 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    17461 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    17521 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    17581 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    17641 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    17701 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    17761 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    17821 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    17881 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    17941 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    18001 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    18061 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    18121 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    18181 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    18241 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    18301 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    18361 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    18421 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    18481 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    18541 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    18601 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    18661 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    18721 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    18781 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    18841 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    18901 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    18961 ttgaacaccg tttaccaaga ggttgtgaa
//
LOCUS       DADOOS010000047        18923 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.047, whole genome shotgun sequence.
ACCESSION   DADOOS010000047 DADOOS010000000
VERSION     DADOOS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.047"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            21..1448
                     /locus_tag="J8C37_001370"
     CDS             21..1448
                     /locus_tag="J8C37_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAZ5304821.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="J8C37_001371"
     CDS             complement(1540..1905)
                     /locus_tag="J8C37_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAZ5304822.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="J8C37_001372"
     CDS             complement(1902..2267)
                     /locus_tag="J8C37_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAZ5304823.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="J8C37_001373"
     CDS             complement(2459..2707)
                     /locus_tag="J8C37_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304824.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="J8C37_001374"
     CDS             2706..2834
                     /locus_tag="J8C37_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAZ5304825.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="J8C37_001375"
     CDS             2891..4585
                     /locus_tag="J8C37_001375"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAZ5304826.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4652..4858
                     /locus_tag="J8C37_001376"
     CDS             4652..4858
                     /locus_tag="J8C37_001376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304827.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="J8C37_001377"
     CDS             5236..6369
                     /locus_tag="J8C37_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAZ5304828.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="J8C37_001378"
     CDS             6400..6720
                     /locus_tag="J8C37_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304829.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="J8C37_001379"
     CDS             complement(6749..7213)
                     /locus_tag="J8C37_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5304830.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7346..8599)
                     /locus_tag="J8C37_001380"
     CDS             complement(7346..8599)
                     /locus_tag="J8C37_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5304831.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8628..8945)
                     /locus_tag="J8C37_001381"
     CDS             complement(8628..8945)
                     /locus_tag="J8C37_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ5304832.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8971..9291)
                     /locus_tag="J8C37_001382"
     CDS             complement(8971..9291)
                     /locus_tag="J8C37_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5304833.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9755..11170)
                     /locus_tag="J8C37_001383"
     CDS             complement(9755..11170)
                     /locus_tag="J8C37_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5304834.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11187..12503)
                     /locus_tag="J8C37_001384"
     CDS             complement(11187..12503)
                     /locus_tag="J8C37_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5304835.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12653..13351)
                     /locus_tag="J8C37_001385"
     CDS             complement(12653..13351)
                     /locus_tag="J8C37_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAZ5304836.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13640..14629)
                     /locus_tag="J8C37_001386"
     CDS             complement(13640..14629)
                     /locus_tag="J8C37_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAZ5304837.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14709..15542)
                     /gene="msrA"
                     /locus_tag="J8C37_001387"
     CDS             complement(14709..15542)
                     /gene="msrA"
                     /locus_tag="J8C37_001387"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ5304838.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15717..17351)
                     /locus_tag="J8C37_001388"
     CDS             complement(15717..17351)
                     /locus_tag="J8C37_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304839.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17338..18738)
                     /locus_tag="J8C37_001389"
     CDS             complement(17338..18738)
                     /locus_tag="J8C37_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ5304840.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT         6000 a         3720 c         3162 g         6041 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaaac ggaaaacaat
     7321 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
     7381 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
     7441 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
     7501 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
     7561 cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga
     7621 taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt
     7681 ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac
     7741 ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga
     7801 taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat
     7861 tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga
     7921 atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg
     7981 gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa
     8041 ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg
     8101 cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc
     8161 ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt
     8221 ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg
     8281 cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca
     8341 agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt
     8401 caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca
     8461 ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg
     8521 caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa
     8581 atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat
     8641 cgacgataca gattaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga
     8701 tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt
     8761 gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt
     8821 ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg
     8881 ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct
     8941 tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc
     9001 gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat
     9061 gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa
     9121 aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc
     9181 aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag
     9241 acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac
     9301 gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt
     9361 taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga
     9421 aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa
     9481 aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg
     9541 catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat
     9601 tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag
     9661 ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca
     9721 gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat
     9781 ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc
     9841 ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg
     9901 gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg
     9961 ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca
    10021 cgattccttt ctcatccata tactgttctt catattctct tcccataccg ttttctgtta
    10081 tcagccaagg gatattatga taattttctt ttagatacat ggctatatca tacatgattt
    10141 tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt
    10201 cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa
    10261 cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt
    10321 ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact
    10381 cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat
    10441 acatataagc agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat
    10501 ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag
    10561 ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg
    10621 gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa
    10681 accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa
    10741 aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt
    10801 cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt
    10861 cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac
    10921 gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat
    10981 cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa
    11041 caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt
    11101 tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag
    11161 taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact
    11221 tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact
    11281 gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg
    11341 accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca
    11401 gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata
    11461 ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt
    11521 tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact
    11581 ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact
    11641 tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc
    11701 cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata
    11761 aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg
    11821 acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca
    11881 gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca
    11941 ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa
    12001 taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta
    12061 tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa
    12121 acagttggat taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc
    12181 actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa
    12241 cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag
    12301 aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca
    12361 aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata
    12421 taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata
    12481 aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct
    12541 attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata
    12601 taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag
    12661 tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca
    12721 ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa
    12781 tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg
    12841 attcattaat gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca
    12901 tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt
    12961 caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg
    13021 ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc
    13081 gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac
    13141 tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca
    13201 cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg
    13261 gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag
    13321 ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat
    13381 tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga
    13441 ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag actaggataa
    13501 aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata
    13561 acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa
    13621 aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg
    13681 agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc
    13741 cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta
    13801 atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc
    13861 caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa
    13921 taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc
    13981 ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca
    14041 tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca
    14101 ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc
    14161 ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat
    14221 gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg
    14281 aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata tgataacctg
    14341 ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc
    14401 caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg
    14461 taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat
    14521 caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg
    14581 atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc
    14641 ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag
    14701 aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt
    14761 tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt
    14821 ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt
    14881 gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct
    14941 aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct
    15001 tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg
    15061 ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa
    15121 ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa
    15181 acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct
    15241 gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat
    15301 ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc
    15361 agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta
    15421 acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct
    15481 cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc
    15541 atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa
    15601 agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa
    15661 tccttatcga cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac
    15721 cttttatgga aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa
    15781 gaaataatag tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact
    15841 ccgcttattt gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt
    15901 tctacattcg ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta
    15961 gttactctat atatgataga actaccagtt gttttataag gaaggtttac ccattgactg
    16021 ccaatctttg cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa
    16081 taatcatata aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt
    16141 acaacaatat gtcccggctg agaaattatt ttttgattga aatttttttt actagaaaca
    16201 gttaaagtga ttaataacaa aacagtcata aataaataaa atagttctct tgtcttattg
    16261 tatcttctat ttttgaaaaa tatcatcgtt aatatcagag aagagaatat tatttgcaag
    16321 cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg tattttgcag aagataccat
    16381 ggcaccaaac tagttgtagc tagccaagtc aaacacgtac cataataaat ctttttttct
    16441 atgtttgtta attttttaaa taaaatcagg ggaatacata aagataacag accaactagt
    16501 cccattgagt aagtagttaa gtcgttgttc atagcttcag tcagactatc gaacacatta
    16561 gatgcatgag catataaatt tgtcacgcta ggtttgttga ttttttggta gagcatttgt
    16621 tcaaacatcg gataccaaaa agcactcgtc agaaccaatg tcattcctgc cgcactaaat
    16681 aattttttaa atatagcaat aaaatctgca ttcttgaaac ctttagaaac caaacgaata
    16741 aaaataaaaa tgataattaa tgctgtactc attaatgttg atagtacatg gctgtacact
    16801 agcaaactca tactcaatgc tagctttaca gacggatact tttcttcttt tccagagatg
    16861 atttgataag cataaaataa tacagggata acaagtgtta ttgcgatagc ttcggcaatt
    16921 gcggaacgat agtagatgtc tactgtccga taagcagaga aaatatagag acaactaaat
    16981 aaaaatgccg cttttttttg ttttaaaaac cgctcgccat aatgataagc tacgagtgtt
    17041 gtacacaagt tcaataacca aacgtaaata atatagctta caatcaaatt ttttgtcatc
    17101 caatataaca ttacagctgg aaaaaaagtc agaaagggat aaaaataatt tacaccatat
    17161 ccagtataat tgaatgtctc ataattaatc ggactggtaa aaatagtcga taaccccttg
    17221 attcgagata agtgaaaaga aagatctcca gtggtataaa tactctgtgt atttttgaaa
    17281 aagatgaaag aatatatact actaactata attaatccac tcaatattac tactttttta
    17341 tttattttca ttcttcttgt ttatctcctt tttagtcaca tatcgagaat ctttcaactt
    17401 attaggtgca aatcgtttaa aacttgaata caccactaaa caataagtca gaatactgat
    17461 ccataaagcc atgagagcag ttggagtata ttttgttgta atttctatct tattttctcc
    17521 agcatttaca tccacattca ctaagccatg ttttttataa gagtcattta cgactttacc
    17581 gtttactttt acctgtgtac ctaaataagc atatactggt actgtcactt tttgcttttt
    17641 gttgcttgcc actgtgtaag taatgttatt atctgcagct ttatacttcc ctttttccca
    17701 cgattctcct acttttactt catgtttttc taagtgaggc ctttctttta tcgcttctgt
    17761 tggggcgtaa tcgtataatc ctcccatatc tgatgttgta taacgtggga gtgtttcttc
    17821 attgataaca atatgaccgt gttttgattg gataactttt gcgtaatttt cttttgctgc
    17881 tatagacgtg acgagtagaa acaccacagt agcgccaatg attaaccatg aatatttttt
    17941 attcatagtc tttttcataa aaacaagcgt tatgatcatt gaagaaaaaa agatttggat
    18001 acctaagata cgccaaggaa actggagtaa gcttatcggt gttttttgaa acaaaaacca
    18061 tggaaataaa ttagttacgc atacccatgt agaaactacg ccaacataga tgattttttc
    18121 ttttttctcc atttctttca gtatgaataa aggaaaaatc aacgcaacca atcctaataa
    18181 ccccatagtg tatgttgtaa tatcgttatt taacgcttga atgatactat ctgaaataga
    18241 aagtgcttct agctgcaaat ctgttttgaa gggactgtgt atttcttgat aattcatctg
    18301 ctgcaacata ggataccaaa aatagctagt taatgcacaa gtcaaaaaaa cagcctttac
    18361 taaattcacg accacaaatt ttatatcgtt ttttatttgc ttgcttaaaa acaaacgaac
    18421 tataatcata caagcaatca atgcactaca caatagtgta gttaagacat gagtatacac
    18481 aagtagactt atactgacag ataaagcgat gacttttttt tctctattga atatcaatcc
    18541 ataagcataa tataaaacag ctgggataat cgtaagagcg attgcttctg caattgcgga
    18601 tcgataatag atatcaactg ttcgataagt tgcaaaagta tataaacaac taaataaaaa
    18661 agcagcatct atctttttaa aaaacttttc tccataataa aaactaatca gaatcgtaca
    18721 aatatttaat aaccaaatat atagaacata acttaatatt aagttatttg aaattccata
    18781 aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa tagttcagtc catctccata
    18841 attgttaaat gttgtatagt tgattggccc ttcaaaaata gaagataacc ctttgattcg
    18901 tgaaagatga aaagataaat cac
//
LOCUS       DADOOS010000048        18838 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.048, whole genome shotgun sequence.
ACCESSION   DADOOS010000048 DADOOS010000000
VERSION     DADOOS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.048"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(146..1111)
                     /locus_tag="J8C37_001390"
     CDS             complement(146..1111)
                     /locus_tag="J8C37_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAZ5304841.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="J8C37_001391"
     CDS             1345..2190
                     /locus_tag="J8C37_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ5304842.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="J8C37_001392"
     CDS             2381..3568
                     /locus_tag="J8C37_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAZ5304843.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="J8C37_001393"
     CDS             3574..3930
                     /locus_tag="J8C37_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAZ5304844.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="J8C37_001394"
     CDS             3932..4270
                     /locus_tag="J8C37_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAZ5304845.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4665..5699
                     /locus_tag="J8C37_001395"
     CDS             4665..5699
                     /locus_tag="J8C37_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5304846.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="J8C37_001396"
     CDS             5692..6378
                     /locus_tag="J8C37_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5304847.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="J8C37_001397"
     CDS             6402..7250
                     /locus_tag="J8C37_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304848.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="J8C37_001398"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="J8C37_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAZ5304849.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="J8C37_001399"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="J8C37_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAZ5304850.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="J8C37_001400"
     CDS             9516..10751
                     /locus_tag="J8C37_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAZ5304851.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="J8C37_001401"
     CDS             10738..11208
                     /locus_tag="J8C37_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAZ5304852.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="J8C37_001402"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="J8C37_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAZ5304853.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..13841)
                     /locus_tag="J8C37_001403"
     CDS             complement(12699..13841)
                     /locus_tag="J8C37_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5304854.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13880..14614)
                     /locus_tag="J8C37_001404"
     CDS             complement(13880..14614)
                     /locus_tag="J8C37_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304855.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14846..15121
                     /locus_tag="J8C37_001405"
     CDS             14846..15121
                     /locus_tag="J8C37_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5304856.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15219..17216
                     /locus_tag="J8C37_001406"
     CDS             15219..17216
                     /locus_tag="J8C37_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304857.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(17292..17627)
                     /locus_tag="J8C37_001407"
     CDS             complement(17292..17627)
                     /locus_tag="J8C37_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304858.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            17754..17969
                     /locus_tag="J8C37_001408"
     CDS             17754..17969
                     /locus_tag="J8C37_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5304859.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            18006..18368
                     /locus_tag="J8C37_001409"
     CDS             18006..18368
                     /locus_tag="J8C37_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAZ5304860.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            18414..18740
                     /locus_tag="J8C37_001410"
     CDS             18414..18740
                     /locus_tag="J8C37_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304861.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         6242 a         3262 c         3936 g         5398 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa tcataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13681 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13741 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13801 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
    13861 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13921 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13981 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    14041 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14101 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14161 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14221 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14281 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14341 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14401 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14461 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14521 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14581 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14641 tacctcaaaa aaggggtgtt gaaaataaat tttacaaagc tgttgacacc ccttttttat
    14701 cgaggtatta tgtgtatgtc gatgaactgg taatcatttt ttgtttgatt aatcggttaa
    14761 taacaaaata cttttatttt gagcaaaaaa tacgaagata attcttagaa aatttgttca
    14821 agcaatcacg aaaggagggg gaaatatgtc tctacaaatc attgatagca ttgtttctat
    14881 acaagttgaa caacaagtga aagagataat tacagtgcta aagcctatgc tagaagacat
    14941 tatttctgga gtcactctag agtatatgga ctatcagcag gcaagtgaat atctaggtgt
    15001 atcagtagga acgattagaa aatatgtttc tcaatacggg ttacctgtta taaaaattga
    15061 tacggtcatt agatttaaaa aatctgatat tgatagtttc atggaacggt ataaaggttg
    15121 agtaaacaaa taagactaaa acatatagta ataaagagtt tgtttttatt atctgtatgt
    15181 tttagtcttt aacagaattt tataaggagg aaacacaaat ggaaacatca atgattaata
    15241 ttccaattta tgtagatcaa gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa
    15301 ctgatgtaag agataggtgt ttagataact atagagaagt tacaagagat gagtttgtag
    15361 agatactaaa ttcatcacaa tcatttattc caagcaagac aaagaataaa ggcaatgaca
    15421 aggagagttt tgtggaaacc agagtaatta ttctggatgt cgataacact gttaaagatg
    15481 agaaggggaa ggtagtagat ttgagtaaag atgatagtag atacttaagc gtagaaaagg
    15541 ttttatctat caatttagtt cataattctg cgtttgcgat acaaaaaagt attcggtact
    15601 ctgaaaatct agaaaaatac aagattgtct ttctattaaa agaagcaatt actgactatg
    15661 gtaaaatggt tgctgtttac gaatacttga aaaagaacat ccccgggtgt gatgacaatg
    15721 taaatgcttc gaatagaatg ttttttggag gatataagtc agatgcagtg ataataatta
    15781 atgaagacaa catgttagat attactgagc ttccgattga ttttaccatg acatgttcta
    15841 aaaattgtca aagtgattca gggagagagt atgaactagt caatgaaacc gattttgtta
    15901 agttgataaa aaatgacgac aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg
    15961 attcctccga catgtctttt aatgagattt atgaaagatt gttaaccatt gatatgaata
    16021 aattacttaa gtcaagtaaa aacttaagat gtctgtttca caacgatcat aatccctctg
    16081 ctagtatttt taccagtaaa actgggcatt ccatttttta ttgtcattcc agtaattgtg
    16141 atatcagtga aaattttatt ggagttgtga tgctaatttt aaagaaagac agtcgggtag
    16201 aaacatttaa ttggctatta gagaacctag acttgtatcc tacacatttt cgaaaattga
    16261 aagaggaatc aaaagtactt tttgacactc ttgaatcaat gaaagacaaa cttttcagga
    16321 caataaggaa ttatctagag gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta
    16381 atttttttga tgatagtaag gaaagtgtga cttgtatttt gtcgggggaa caattagcta
    16441 aaaaaatgag ttcttactat ggtaaaactt atgacataga taaatgtaat aagttactat
    16501 cacttatgac ttttatagga ttgataaaaa aattagatga tgaagatgtt cctttgaaga
    16561 tgctcgaata cctaaatagg ttaaaggaag agaaagcgtt aggaaacttt aatcataaga
    16621 gaagcaatgt ctatcgatta gacgttttta atgcaacttc tgtgattcaa aaattgaatg
    16681 aagatatcct tccacttctg gaaaaaattc attttacata tcaatatttt tcttatgaat
    16741 gggttaagat atgttttaac gaaaaagagg ctaaaagagt atttcctcaa aattcccgat
    16801 cagaactcag caaggaaaaa caattgattt tagatgaagc acatgaagtt ttacagggct
    16861 tatcgtggca agggacatat ctaatgacag agaaagaact tattaacact gtttgtgaac
    16921 gcctacgtaa tgttggggat aacagcgtga aaaatgcttt aataaaaaac agaggctact
    16981 ttgttaaaca aggattcact cttgaacgag cttctaagga tattaagaaa ctatacaatg
    17041 tgaaagcaag tagtagtttt ttaatatatt tagcgaaagg ggcagggata gatgaaacaa
    17101 taaaagaatt gatattatca ggagctggga tagttcctct gtatcaattt acaagtgtaa
    17161 caagagatga tggacggaga gtaaggaaga gaaaagaccc aattgtatta aactagacag
    17221 gttatccata gctcttaatc ttatttcatc tcaatacacc caccgttaaa gttcggtggg
    17281 ttatttattt ttcaatcttt caatagctga ttgcgttcat tttcttgcct ttttaatagc
    17341 ttcatatagt caattgttcg aactgcatct tcagtgctaa gagaacgata gagagccaaa
    17401 aactcttcct cataatctac gacaacattg tctagaaaat ggcttacagg ggttcctagg
    17461 gctctagaaa gagctgttaa gctatcgagg ttaggctctg ttacagaatt ttcccatctt
    17521 gctactgcct gttgtgtaga gaagattttt tcagctaatt ctttttgtgt ccacccgaga
    17581 gcttttcgct tttctctaat gacctttgca tattgtttct gtttcattaa tacaccccca
    17641 tctcagtata gtacaacaag ttgttgtatt tgtaatggta tttgaagttt tttatatttt
    17701 atgagttgaa atacatcata ttgttgtata taataaaaca ggaggggaga atcatgaaaa
    17761 aagtaagtaa tctcagccga tacagaaaag aaaaaggact ttctcaaacg gagttagcaa
    17821 aaaaaatgaa tgttacacag caatgtgtaa gttcgtggca aacagggaga acgattccta
    17881 aaccctatca aatgaagatg ctttctgaga tattaagtgt accaatgaat gagctgtttt
    17941 ctgatgtatt taataaagta aacagttaac taataagcaa ttgaatgatt ttaggaggag
    18001 aagacatgta ttgtagaaaa tgtggcaaag aaataacaga tgaaagtaag tattgccagt
    18061 tttgtggagc actacaagaa acagatgaga caaccggaac agacacagta aagaaaagta
    18121 ctgagcagac aattaatgaa atgaaggaac aaatcgtaca tggacaagag acagggaagc
    18181 tgtatttgat tattggttgg atttctatgg tagtatcgtt agcatttatt cctgttgtat
    18241 ttggtgctgt tgctgttatt atgggctact tatatcgaga aaaagatgag aaaatgggta
    18301 cgattttgat gatagcaggg gtagctggag caatctttgg tgtactttta gggatgtcaa
    18361 tgtactgata agagggataa gagcaaatta ttttggagat aggagataaa aagttgaata
    18421 aagtaaatat gaacaagaag tttgtcatta taggtattgt tgtcttggct attataggta
    18481 ttattttttc aaaaatgaca ggtacatctg gattagagaa gtcatcaaaa ccattggtaa
    18541 cacagatttt acaagaacag tacaacttaa atcgatcatg tgatgatgtg acgattactc
    18601 atgaaaatgg agataacaca taccgggcta aagctatatt agacaatgga agtgctatca
    18661 acataaatat tgagtactat ccaaagaaag accgtatata tgtggagata ccatatgctg
    18721 aggtgttaat gttaaattga tttaaatgta ttatgcatac cgtccaaaca ggaactccca
    18781 aaggtgtaaa ttcactaggg agtttttata gtttcctata aattaaatac taatacag
//
LOCUS       DADOOS010000049        18527 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.049, whole genome shotgun sequence.
ACCESSION   DADOOS010000049 DADOOS010000000
VERSION     DADOOS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.049"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(247..1539)
                     /locus_tag="J8C37_001411"
     CDS             complement(247..1539)
                     /locus_tag="J8C37_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ5304862.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1536..2159)
                     /locus_tag="J8C37_001412"
     CDS             complement(1536..2159)
                     /locus_tag="J8C37_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304863.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2162..2998)
                     /locus_tag="J8C37_001413"
     CDS             complement(2162..2998)
                     /locus_tag="J8C37_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304864.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3015..3902)
                     /locus_tag="J8C37_001414"
     CDS             complement(3015..3902)
                     /locus_tag="J8C37_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304865.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3910..5145)
                     /locus_tag="J8C37_001415"
                     /pseudo
     CDS             complement(3910..5145)
                     /locus_tag="J8C37_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5828..6511)
                     /locus_tag="J8C37_001416"
     CDS             complement(5828..6511)
                     /locus_tag="J8C37_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAZ5304866.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6529..7146)
                     /locus_tag="J8C37_001417"
     CDS             complement(6529..7146)
                     /locus_tag="J8C37_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAZ5304867.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7156..7980)
                     /locus_tag="J8C37_001418"
     CDS             complement(7156..7980)
                     /locus_tag="J8C37_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAZ5304868.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7973..8533)
                     /locus_tag="J8C37_001419"
     CDS             complement(7973..8533)
                     /locus_tag="J8C37_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304869.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8707..9663
                     /locus_tag="J8C37_001420"
     CDS             8707..9663
                     /locus_tag="J8C37_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ5304870.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9968..10924)
                     /locus_tag="J8C37_001421"
     CDS             complement(9968..10924)
                     /locus_tag="J8C37_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAZ5304871.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11028..11630
                     /locus_tag="J8C37_001422"
     CDS             11028..11630
                     /locus_tag="J8C37_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ5304872.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11718..13217)
                     /locus_tag="J8C37_001423"
     CDS             complement(11718..13217)
                     /locus_tag="J8C37_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAZ5304873.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13233..13757)
                     /locus_tag="J8C37_001424"
     CDS             complement(13233..13757)
                     /locus_tag="J8C37_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304874.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14055..14804
                     /locus_tag="J8C37_001425"
     CDS             14055..14804
                     /locus_tag="J8C37_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAZ5304875.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14795..15580
                     /locus_tag="J8C37_001426"
     CDS             14795..15580
                     /locus_tag="J8C37_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ5304876.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15549..16265
                     /locus_tag="J8C37_001427"
     CDS             15549..16265
                     /locus_tag="J8C37_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAZ5304877.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16367..17386)
                     /locus_tag="J8C37_001428"
     CDS             complement(16367..17386)
                     /locus_tag="J8C37_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAZ5304878.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17405..18463)
                     /locus_tag="J8C37_001429"
     CDS             complement(17405..18463)
                     /locus_tag="J8C37_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAZ5304879.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         5546 a         3900 c         3254 g         5827 t
ORIGIN      
        1 ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg cattattcct
       61 ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc agcccacata
      121 aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat agtgttcaaa
      181 agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat ttccgtaaat
      241 ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc ctgaagcaat
      301 agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt gagttgctgc
      361 ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata aacgcggatc
      421 tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct caccgaacat
      481 cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat ctccacctgc
      541 attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc cattgaaaga
      601 aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa tcgcaccagt
      661 ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg aaaatacact
      721 gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc gcaattcctc
      781 attggctgac aggtctatag caaatggatc agctttagct agttcgcttc catagcatcg
      841 actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa agtttaatcc
      901 accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta gatcaaaatg
      961 atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat gccatcttct
     1021 gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag aaaacacatc
     1081 accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg cataacgata
     1141 attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta tttcttgagc
     1201 tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt ttttcattgc
     1261 acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt cagccggccg
     1321 tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt ttttctgtcc
     1381 atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata catcacaaaa
     1441 accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa aaaaacgcgc
     1501 tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt ctccttttct
     1561 tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt gagtaaaaaa
     1621 tcaggctgta tacaccagaa cagtccatac aaaaggcata aataccacag aaaccccttg
     1681 acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact atctgcttta
     1741 tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa aagcaacaga
     1801 cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa ataccaatca
     1861 actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat ccgtttgatt
     1921 ttccctttcc tccaacatac ctttttttct gccaagtaat ggacagatag ataataagaa
     1981 ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac taatcctaaa
     2041 gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact ggttaaaaaa
     2101 atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg ctttttcata
     2161 ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg aagaataaga
     2221 aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc gcgtaagtcg
     2281 tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa gatctcggct
     2341 tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac tggatgatga
     2401 tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag acactatact
     2461 tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg accatgaatt
     2521 gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc agcggcacat
     2581 acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa atcacttgtg
     2641 caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct ttgaaatcga
     2701 ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc gtcgaaactg
     2761 cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca aaccccgccc
     2821 atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc gaaccactca
     2881 taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga taaatcatga
     2941 ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta ttattcattt
     3001 cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat cagaaaggtg
     3061 acagctccca taatcaacag catgatccac gaaatagcac tagcataccc catagaatta
     3121 aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc attcggacca
     3181 ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt ctgcataacc
     3241 aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa cgtctgccat
     3301 ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc tttgatacct
     3361 gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac aagagggatc
     3421 atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg ttccaggaaa
     3481 atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat agaaatactg
     3541 ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc tttgatattc
     3601 ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac agatgcaaat
     3661 acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag catctttata
     3721 taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt gaacgaataa
     3781 taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat gaatggagaa
     3841 gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt ttcttttttc
     3901 atgccttcct tctgctgtta cttcttcata aatgactttt tcaactaaaa gattgtattg
     3961 gtcttgaatt tccggattca aagcactatc aaagacattc acttcgccgt tgtccatatc
     4021 ggaaatcatt tctaaataat caaatgtcgc ttgcaaagtt gcatcatctg cacgagcgag
     4081 ctgttctcga atattcgaca tgattggtac accacgttct ccttttaaaa tttcattggc
     4141 ttcttcactg ttttgaaaga aatcaataaa cttagcagct tcttctttat gggctgagct
     4201 ttttgccata ctcagcattt gcgaagaacg taaagggata ccagattctc catctgcttt
     4261 tctgcgaggt ggattaatca actgaatctc catatcttca ggagcccctg acaatatttc
     4321 tgtcaactgg ttacttgata gaaacaccat tgccgcttct ctatccctaa ccgcctgaaa
     4381 agacagatcc gtcgtacttg cagctccacc acctggttct ggatgggccc ctttgtctac
     4441 cagattttta cgcagctgga aaaaatctgt tagatagtct ggattttcaa atcccaagct
     4501 gttagataga tcttcccgat tataaaaatt caatcctttc tcaacttgtg gtatttgaat
     4561 aatacagcca gctataaaat cttcgaatct tgaaatagca tagattccta attcttcagt
     4621 gatttgatag ctgatttgtt caaattcatc ccatgtccaa tttgaagctg gttcagggat
     4681 atttaattgt tcaaatatat ttttattgta agcaatcgca aacgaattca ccccgttaga
     4741 cagtccaact aattgcccgt cgtaagtcgt cgttgctaaa taggattcat ctgcattaga
     4801 aatatcaatt atcccttgct ccacatattc atctaatggt tcaatctgat tgatgtattg
     4861 aggaaattga ttgttcagtt ggaaaatgtc ccagatatca tctgatgctg ctagtgtacc
     4921 taattttgtg taatatcctt cataatcata aaattcataa tcgatcgtca catggggatt
     4981 tttttcttca taaagctcga ttacttttac tgttgcatcg tgtcggactt gcgaacccca
     5041 ccaagccatg ttcagcgtga tttttttgtc atcttcttgc ccacaagccg tcaagaagaa
     5101 taacataaca cttaaagcta agatgattct cattcttttc ttcattctct gcacctctac
     5161 ttccattgtt atttttcaat atactgtatt tttatacaga acatatttat taaacgatct
     5221 ttttcaaggg gaataaaaac caagacttgc cttatcgttc tgcctaggat gaacaactca
     5281 atactttcaa gaaaaattag atagtgctga ttcttctttc atcgctaaaa acacttcttg
     5341 cttcaatgat gggaggataa ttacgcaaag aaaaaaggat atcagacaac tgcccaatat
     5401 ccttgacata gctaaataag catgcttctt cgtgtgcgct cttccctaaa aaacagaaaa
     5461 ataactagta attctgtatg aaattaggta caatatacac gtcgatgcac aagcattgac
     5521 tatggcaact tgattggtca gttcccgctg acttttcaag ccccgacaat ttagctgcta
     5581 ctaaattgtc gggtgccttt tttagttttt attcgttttt cattctatag aaagcgcttc
     5641 aataaatcaa gacatttttt tgttagaatg ctaataataa caaaaggttt taactcttct
     5701 tatgtacatt acaggtaaaa aaacaaaaaa atttctcttt tttttgaatt ctgaacaaat
     5761 agtactggag tacaaaagac gaggaagagc cactatctgc tcttcctcgt cttttgattc
     5821 tcacctgtta ttcatgattc tttttctcag tttcccgata tttttccatt ttacgactaa
     5881 aaagctctcc gctacttggt ggcgtatatg tgatggcatt tgctcctgct tgaatggtct
     5941 cagcaatact ttcttccgtt ggaccacctg tcgcaatgat aggaagttca ggatattttt
     6001 tacgaaaatg gcgtactgct tctgcagttt ctttacctgc actgatattg ataatcgaaa
     6061 taccagctgc taacttttca tctatttctg tatatttcga cgttaccgta ctgataactg
     6121 gaatatcaac tactcgacaa acttctttta ctgtctctaa aggcgtagga ccgttcaaaa
     6181 cgacccctaa cgaaccttgc gcttctgcga acatgctcat gtaggaagaa cgagcacctt
     6241 gtgtcaaacc accacctaca ccggagaaaa ctggaatgtc agctgcctca ataatgcttt
     6301 tagtaatcgc tggatgaggt gtaaaaggat acacagcaat tactgcattc gcatttgtat
     6361 tacgtatgat cgcaatatcc gtagtaaaga tgatcgaacg gatcaatttt ccaaatatct
     6421 tgatcccgct tgcttctaaa atgatttccg gtactcttac gatatctttt ctcaattccg
     6481 tcatgatttc tggcgtaaat tgttctgcca tcagccactt ctcctttgct atctccggtc
     6541 atactgattc aattgatacg tctcgatgac ctggatgatt ttttgcgcat aaccggggtc
     6601 tgtcgcataa cctgcatctt gcaatgcttg tgcggcttcc tgatagttgg tggccgtaat
     6661 cactttttca tacagcgcag gattccaatc tactccttgg acgaacagtt gtgtatggtc
     6721 atccatggac tgttcccatg aatcatacac tttgaagtct ccttggatag ttatccattc
     6781 ttcgttaaca aattcctttg tttccaggct tactttcttc tgatccccat aagccttgat
     6841 cccgaaaaga ttattgtatt tgctagccaa ggtacttttt ccccaatttg attctaatat
     6901 agcttgtcct aaaatgatac taggaagcac tccataacca tcttgtagtt cttgggcatg
     6961 cggaacaagc cgttcaataa attgctgatg ggattgcgct tcattttctt gctgattatc
     7021 agtaaaagga gaagacagat tcttcaagga aaaaacaaag gctaagagta tcactgctac
     7081 accggcaacc actgctggcc aacggatatt cttgcgcttt tgctttttcc actttttttt
     7141 cgccatgatc aattcctact tttcatttaa ttcattcaga tattcttcta gcgtcaattg
     7201 gtgtttggtg atatactcag cgttttcttt accgacataa cgtaagtgcc aaggttcata
     7261 tgtgatctta gttatatctt cgtgtccttt tggataacga acgataaatc catactgcgc
     7321 agcattatct gcgatccatt tagctccagg ctgatctccg taacttgcat ccaatacagt
     7381 agaaggataa ctgttgtacc agttttgatc gacgacatcg actgccagac ctgtgtggtg
     7441 ttcgctgtat cctggctccg taatcgtttc tttagtaatc tttgtcgcct cctcttctga
     7501 tacaccttga cgactcatca catcttggac attttgttcg aaaacttgtt gttgggatga
     7561 tacagaccgg taagctgaga ccatcactaa tggaaatcca gctgcttcag cagccttggc
     7621 aaatgcttca tattctggag caatgcgttt gtcgatcaag tatccgttac ttaatgatgc
     7681 caattgagta gactcatcaa tttctttttc taatttgtga tctggaccaa ccaaaactag
     7741 cttccaatcg tctgttttaa catctggcaa ttgtttggac gtacgatctt ttgtttcttt
     7801 gtcctttacc ttgctgctgg ttgtttgact gttcttttct ttactagcag ttgttttggt
     7861 gcttgttcgt tcgactgctt gattgccggc ttgaacatgc gaatcatcat cgctaaaaat
     7921 aacatagccc attgctgcga tcataatcac aactgctcca aatcctaaaa atttattcac
     7981 ttgaaaaatc ctttcgttgt tcttcacgtt ccccattcgc ctcatgaacg taagtttctt
     8041 ccagattttc attcacccaa tcgagaagtt cgattttttc tccttcgatc tgtttttgaa
     8101 catctgctgg cagacgaaaa ttcaaaatat catcatagcc ccattcatca tattgaatac
     8161 ttactttcaa gtcaaggtat ctaccagcct gctgattatt tttcaaaggg atcagctcga
     8221 cttttgctgc tttgctgcta agccattttt gcgcgatgcg tgttttgatt tgcttgtact
     8281 ctgcaacggc aacgtttcgt ttttcgggaa aaataatcgt ttgacgcaaa atcttttgcg
     8341 ttagcatcca ttcataatca gtgaaaagat tttttacttt ctgcatcgct tcagatggca
     8401 gatttttctc gccatctttc catttttgcc attgttctgg actgatcagc aagtattcat
     8461 ctaaaaaatt tttttcagtt tcaaactgtt ctaataatac actcataata agatcaatat
     8521 atacttgatg cacgtttatc ccctcctttt aactattcta cttaagtttt gatcaaaaag
     8581 cacctcttaa atcgctgctt tcattttttt taatgtttca agtcctctcc tctttattct
     8641 tcgtgatttt tgttataata atagacagac aagaaactca atccaactat gaaggagcgt
     8701 tttcagatga aaaaatatca atggggaatc gttggtcttg gtaccatcgc acatgagttt
     8761 gccgaaagct tcaatcaaga gacgagcgaa ctaacagcag ttgcttccag aacttcagaa
     8821 aaagcggaaa acttcgctca ccgttacaat attccaaaag cttacggaag ctatcaagaa
     8881 atgttagacg atgctgaaat cgacatcgtg tacattgctg taccaaatag acagcatatc
     8941 gaccatatct tggcagcatt ggaagcaggc aaacacgttc tatgtgaaaa agccatcacg
     9001 atgaataaaa aagaactggc tgacgctatg cgcttagcag aagaaaagaa tttgatctta
     9061 gcagaagcga tgacaatctt caacatgcca ctttatcagc agttacgctc aataatggat
     9121 acaggaaaac tgggggcttt aaaaatgatc caagcacctt tcggcagtta caaagaacca
     9181 gaccctaaaa accggttctt caatccagaa ttagctggag gagctctgtt ggatatcggt
     9241 acgtatgctg tatctttcgc tcgtttcttt ttgagctctc aacctgaagt agttgcctct
     9301 acaatggttc cttttgaaac aggcgtggac gaacaatccg tcactatctt gcgtaacaaa
     9361 gaaaatgaac ttgctgctgt cagcttgact tttcaggcaa aaatgccaaa agtcggtatc
     9421 gttgcctttg agaacggtta tgtgacgatt gctgattacc cgcgtgctga tcgtgcggag
     9481 atcttattta cagatggtac aaaggaattc atcgaaagcg ggaatacatc tgaagcaatg
     9541 aattacgaaa taaagaatat ggtacacatg attgaaggtg acttgcccaa tcgctctctc
     9601 ttcttgacaa aagatgtcat cgaaatcttg gatcaaatgc aagttctatg gaaaaatatg
     9661 taattttaaa aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa
     9721 aatagctcct cctatttttc gtttggctgg acttattacc gcagaggaaa gcttttgaac
     9781 accgtttaag aaagaggttg tgagcctacc gttcagctcc gagtcacaag gagcaatttc
     9841 aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag ttgacaggag
     9901 aacaccgttt aaagaagagg ttgtgaaatc agctttttga cctgaatatt agctgaattc
     9961 acaacctcta tttttggatt ttttgttggg agaattctgc cttgatttca ataaaacttt
    10021 taaaagactg caaaaactgg aacaattctg ctcgatcctc gaattcaggg cgactagtcg
    10081 gcagaggcat ttgccgatac aatacataaa cttcttcaat acgtgttaaa atttgtctgc
    10141 catcatttga ctctgaaaat gtttctgcag tataaatcaa taaaccatag atatgttttc
    10201 cataataagc tggttgctgg attctctcta gattttccag catgtcttta atcacattgg
    10261 cctgtgctct tctcatagag aagtaagttt cgtagtatag cggctgtctg agccagtagt
    10321 tttcttgatg ttctctggca tcttcttggc tttcgcgaat gaatgtcagt aaatgttcgc
    10381 atttgatctg cacatcttct ttattttctg ataaaagaaa ttctgccatt tgtctcaaaa
    10441 tatttcgaaa ttctttttcg atctgtattt ggttttcctt cagctgcttc gtacgatctg
    10501 gcatgtaaac atttgcaagc aaagcaaata ctaatccaat cgccattaat cccgcttcat
    10561 tgccgatcag ctgccaagac atgttttcct ccactaaata atgagtgacc aaaaccgaat
    10621 ttactactat cccttcggtt aatttgaagc gagaggcaag cgggatgaac aggaataaaa
    10681 aaagactgaa gccaaaaata gtatacccaa cagcaaaaaa actgaacagt gctgttgccg
    10741 tagctaatat aaaggctgcg attcgattca tgcccgtacg caatgtagag cgcttggtat
    10801 ttcctacact taataatgca atgatccctg cagctggcca atacaacagc gataagctac
    10861 ccgctgtgac catcgaaaag aagacactga tcactgtttt gactgttcgc attcctatac
    10921 gcatttactt cccccttttt tattttaatt atacttgaat aatgaaaaac tagctatcag
    10981 acaaaagttt aggtatgata gtaaaaagac caaaaaggag aatctctatg caatttggcc
    11041 gttttcgact aggtatgcgt acgatcaaaa gtgccttagc tgttttttta tgtatcttgt
    11101 tttttcattt gactcaacga ggaatcccga tgattgctgc attatctgct gttttttctt
    11161 tgcgtcagga cttgactaca accgtttctt ttggaaagtc taggatcatc ggcaatacat
    11221 taggtggtac attagctatc atttattttt ttgtcaaaag ctattttcac aatgattttt
    11281 tggtagaatt gatcttgctt cctattctag ttattattgt gatcgtcgtg tcagatggta
    11341 ttaacaataa ttccggtatc atttccgcaa tagctacgtt gttgctgatt gcgttgagca
    11401 tccctcaagg cgaatccgct ttgtatgcta tccaacgtgt actagatact tttataggga
    11461 cattcattgc aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa aagaaggaag
    11521 aactatcaga agatttggtc gaactccaaa agaaagaacg attgctaaga acaaatttac
    11581 aagagataga agctaagatc caaaaacaaa agaaaaatag cgaggactaa ttttcttttg
    11641 caataaaata aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt tttgattgaa
    11701 cggtgaagtg tactacttta ttcttccttc atcgagtttt gttctgcctc ttttggcgct
    11761 tcatacatat ttacttggct agtagtccca cgtgtcgaca aattctctac gatttccttc
    11821 aagtcaagtc ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa atttgtggca
    11881 taatttgtca ctcggttcgc tccactggtt tctcctgaac tgcttcctgt atcgacaaca
    11941 gagatttttt cgatatttcc tagcggctgt gctgcttcgc gcatcaattg cggtagcata
    12001 tcgataacca tactcaagac agctgcttcg ccatattcct tgaaggcatt ggcgatcttt
    12061 tgttttgctt cggcttctgc ttttcctttt gctagtgctg cttcggcttc tgcttgtcca
    12121 gctaaacgtg tcttattggc ttccgcttct gccagtgctt ccacacggaa tcgttctgct
    12181 tcggcttcag tcacttcttt gactttttga gcttgtgctt cttgttcttt agcgtaccga
    12241 tcggcatctg cttttttctt cacttccgaa tcatattgct tttcacgacg tgtgatttct
    12301 ttttcttcca gttcgatttg tttttggcgt tctacgattt taatctgcat ttcctgttcg
    12361 attacttgtt gttgtgctcg ggcactttct aaattgtagg cttggtctgc ttttgcttta
    12421 gcaacatctt gttcttgttt gtaggttgct aatttcagtt ctttttcttt taatgattct
    12481 gcgatctctg tttgtcgctg taattcagct gcttgtgatt ctttttctgc ttgagctttt
    12541 ttgatacgcg tttcttttag agcttccgct tctgctatat ctgcatctct tttgacttgg
    12601 gcgattcttg gtttccctaa agagtcaaga taaccgtttt tgtctctgac ctctttgata
    12661 gtgaaagaaa cgatgatcaa ccccattttt gcaaggtcta cagaagcgac ttcttgtacg
    12721 ctttgactga acttatcgcg attttgatag atttcttcta cagtcataga accaagaatc
    12781 gagcgcaagt gtccttctaa tacttctctt gcttcatttt ccagttcttc tcttgtcttg
    12841 cctaaaaact gttctgctgc tgtagcaatc tcttctacag atgaaccgat tttgatgatc
    12901 gaagtaccat cacacatcac cggtacgcct tgttcggtat acacttcagg ggtggagacg
    12961 tccaatttac tcgataatag actgatccga ttcgaacgtt gaaaaactgg caagacgaat
    13021 gcgccacctc cacgcacaat tttgattttg ttattgcttt catcaatgtg cacatttttg
    13081 cttcctaaat aacttccact gatgatcaac gcttcatccg gttttgctgt ctggtacttc
    13141 gctacaaaga cgatcagcaa catcagtaaa atcaaagctg cgattgcgat cgcgataaaa
    13201 acaactggta tttccattta acacacgctc ctttaagctt ttatcatgct ttcataaggc
    13261 atgacataac agattctttc cttgatctca atgatcaata ctttcgttcc ccgctcagct
    13321 gtctgttctt gggggctgta aaaacaagct ggacgactaa tggaaccggt aacacttttc
    13381 aactgaattt ctcccatacc tcgtacaggg atcggtgtga tcacaacagc tacttgtcct
    13441 tccagagttt tttcagaaga cgacatcgtt gattcggcat gtttcatcgg catcaatata
    13501 tagaaattaa gcaaaaatac caacactatc gatatgcctc cactagcaat aaataaaaat
    13561 aaagatgatt gatctgttaa acgctcacct agaaaaccaa ataagcttgt gaaagttaac
    13621 caagggataa taagcatcgg atcaagcggg ccatcaaaat cgaatacatc tccaaaaata
    13681 atcaatagga ctgccaatcc tgcacaaatc agcaatgtat accaataaat cgtctcaagt
    13741 gcgattcccc ctatcatata cacgcctcct tccatttcta cgtctatcat accaatgttt
    13801 gaaaattctg tatagcttct tttaccgact cagtggtgtt ttttatagga aggttgtgtt
    13861 tatcgttgac aatttgttaa atttttgttt tcatgttaaa attttcacaa taaattcttt
    13921 cgaggtagcc tatgtttcct ttttttaaga aaaaaccatt aaagctccag aacacgatca
    13981 tccttgtcgt ttgtttctcc acattcgttt ctttgattgt ttctgccata ttgatcagga
    14041 attttattct ttcgatgaac cgatctagtg tgtcagataa aatctctagc attgctaaaa
    14101 tcgttgccaa tgataaactc atccaaaaaa agctgcagac tggtgacccg acacatgcga
    14161 ttcaaggaat aacaaatgaa atcatgattg catcgaattt agattttatc gttgtcatgg
    14221 accctgaagg cattcgatat tctcacgcag acccaagtta catcggtctt cctttctcaa
    14281 gtcatgaaga tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa aaagaaggaa
    14341 tgttagggtt cggcttccgc tatttcgtcc ctatttatta tgatcaagag attattggtg
    14401 cagtttgtgt tggcttaaca aacaacacga ttgacaatca agtcaaagat ggacaaaaaa
    14461 tgattgtttt ttcattgatt attggactta gcgtaggcat cataggcgcc gtttttcttg
    14521 cccaaaaaat gaaaaaagta ctgcttggac ttgaaccaca agaaatagca gaacagttaa
    14581 gtgaaaaaac gattatcgaa aatgaagtag atgaaggaat cttagccatc tcttctgata
    14641 aaactatcct cttgatcaat acacctgcaa aaaacatttt ctaccaaaca gagcagctat
    14701 tggatttcga agtaggaaaa aaatcagtga taatctatat gacgttttgt ttaaagagac
    14761 gattgagcat caaacgaccg ttcggactca atccatgtat ttaaatggga tagaaactat
    14821 ttctagcgtt acacctcttt ttagcaatga ccgtttttta ggagcagtcg caactttacg
    14881 aaatcgatca gaagtaatga aattaatgga ggagcttggg gggacgaaac aatatataaa
    14941 ctccatgaga gctcaaaccc atgaattcat gaataaactt catgtcattt ctggtttgat
    15001 tgatttgaag aagtatgaag aagtcaccca ttttatccag cagctaaatc aaaattataa
    15061 aaaagagatt ggccaagtct caagtctgat caaagtacct gcaattgccg gttttttatt
    15121 aggcaaaata aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt
    15181 tatccctaaa atggagatgg atgactccat ccattcactt ttgcaagtgt tagggaactt
    15241 acttgataat gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac aggtccggat
    15301 tcatttaaac tatgacacag aagctgaagt attcatcgtg accgtctgcg atggcggtgt
    15361 cgggatatca gaagaaatct cagaaaaaat atttacgcta ggtttttcga cgaagggaat
    15421 caatcgtggg tttggattga atctcatcca ttcaatcgtc aaacgccacg gtggattttt
    15481 agaattttcc aaacaaacaa atggcggtac aatcgtctat ctagaattac caaaaaagga
    15541 agatcaaaat gatgacaaat gtcctgataa tcgaagatga tccaatggtt gcagaattga
    15601 atcgtacttt tgtggaaagc atccctaata tgaacgtaat cgaaagtttc agaaatgtag
    15661 aagaaggaaa aaaatatttc agacaaaaaa acgcagatct gctgctgctt gatgtataca
    15721 tgcctggcga aagtggtatc cattttttat cttggcttag acagcaagga aatgatatcc
    15781 cagtgatttt gatcactgct gcagatgata tcgaaaccat aaagaatgtg atccgctacg
    15841 gtgccttaga ttatttgatc aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa
    15901 agtatcttgc tttctctcag acaatccaaa cgactgaaaa agccgatcag aaattgatcg
    15961 atcaagtatt caaccacacc actcccattc acgaaatacc atcattcatc gatttgccta
    16021 aaggtctgtc aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta
    16081 ttttttcaac aaaagaactg gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat
    16141 atttgaattt tttagtagaa caagatatat tgaacgaaag aatcaaatat ttggaaatcg
    16201 gccagccgct aacctgttat gaattgacaa agaatgggcg gaaaaagctc acagattact
    16261 gttgagtcat cgaattatcc catacaaaat agtccaagta tacagaagta acaactgttt
    16321 acttggacta tttttaaaca ttttattgac gaatatccat gttttcttat cttaattgac
    16381 gggctttagg aagagatacc tcatttttcg tttttccagt ttctattatt tcacgaaagt
    16441 tctcgaaact tgtcgaaatc atatttgata atgcctcatc tgtatttgaa cctacatgcg
    16501 gggttacaag aactctaggg tacatttcta ctaattgctg gatagcaggg tctggaatcg
    16561 tctcccatgg cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg
    16621 caaaaccagc taaatgattg cttttcaaag ctcttaaaat agcttcatta tcttgaagct
    16681 ctccacgggc tgtattaatc aagatgctac cttttttcat ttgagctaaa aatccttcat
    16741 tgatcatttg atcgttttca cctgggatat gaggtacatg aatactgaca atatcgcttt
    16801 tggctaataa ctcttcaagc gagtggaacg tcaatacttt tttagcagct tctgtctgat
    16861 gaatatcgta gccgatcact ttggctccta gtcccttaaa caaaacagct tccgtcaacc
    16921 ctatcctgcc tgttcctatg atccctactg tgcaatttct gatttcacga ctaaacattt
    16981 gctcatccac tcgaaaatct ttgaaagatg ttcggatcgt cgtatacgca gtatttctca
    17041 aaagcatcat ggcaaaagtc aaagataact ctgctatcgc atttggcgaa tacgatggta
    17101 cacgggctac tgtcatacca aaatcagctg ccgcttgtag atcgatatga ttgactccca
    17161 cagtccgtgt aaaaacataa cgaatcccat attcctgcat ttttgcaaga ttggtctcat
    17221 ctgctataca atttcccctc aacaatacag catcatggtc ctttgctgac acgatattgt
    17281 catgtgtcaa cagttcttca taaagactga gatcataatg gtaaatattc aatttttcaa
    17341 aaaaatctat ctcgttcgga cgaacaccgt aacatgcgat tctcatactt tcttcctcca
    17401 tttcttatga aaataaacct atgccatgaa tcatttgtaa tacaacgatc catacaggtg
    17461 tcagtaccac agcaaaaata gttgacagta aggatgcatt ggaggctaaa agtgcctccc
    17521 tatcaaaact gatagcatag gctgctgcca ctgttgcagc tggtgtagcc atcatgataa
    17581 cgattgttgc cagtgcttca aaattgactg gtagaatatt cgttgccgtc aaaactgcta
    17641 gaatcacgat attcaatgca ggtacaaata ccactttgac tagactgtaa taccatgaag
    17701 ttttttcaga tgcagctttt ttgaagctta cttcacctaa tgttgcgccg attgctagcc
    17761 acgccaatgg tgaacttaaa ccagctaaat aatccatcgg ctgaaatagc cacggtgcag
    17821 tctggtcaat tcgcaaaaac gctacttggc ttatctctcc ttctgcacct gtaactgaca
    17881 cttgtggcag atacccttgg aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga
    17941 ttggattgac gaacatcgct ttgatatttt tagcttccat cttcaagcca ctcattttta
    18001 tgtatccata agaatacaaa aagattcggt agccaatatt gaaaattgag gaatacatca
    18061 cgccgacagg accataaatc gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa
    18121 agatcgtcag gacacgcaac gttgtttgct tatccccttt atagcttaag aaaaaaggtt
    18181 ttgaaactag aataagaata atataaataa ctaatcccca aatcaagaca ttgattcctt
    18241 gcatcagtaa ttctgtattg atatcttgca tgaaagctcg aaatgccagt gctggcaaag
    18301 ccactgttaa gacgactttc gatagaattt ttccaacaga ttcagaaaaa atcccctttt
    18361 ttctacaaac aaaccctaat aatataataa aaactgttga agtgattgca ctgacaatcg
    18421 acatatctgt caaagtcgat cgaaaaatgt caaataagtt catctttctc ctcctaatta
    18481 tgttcattta ttcacaataa aaaaagcttt tattttgcta atacgta
//
LOCUS       DADOOS010000050        18114 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.050, whole genome shotgun sequence.
ACCESSION   DADOOS010000050 DADOOS010000000
VERSION     DADOOS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.050"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1283)
                     /locus_tag="J8C37_001430"
     CDS             complement(<1..1283)
                     /locus_tag="J8C37_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAZ5304880.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
     gene            1713..2822
                     /locus_tag="J8C37_001431"
     CDS             1713..2822
                     /locus_tag="J8C37_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAZ5304881.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            2905..3801
                     /locus_tag="J8C37_001432"
                     /pseudo
     CDS             2905..3801
                     /locus_tag="J8C37_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            3817..4641
                     /locus_tag="J8C37_001433"
     CDS             3817..4641
                     /locus_tag="J8C37_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304882.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            4673..5983
                     /locus_tag="J8C37_001434"
     CDS             4673..5983
                     /locus_tag="J8C37_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAZ5304883.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            6131..7390
                     /locus_tag="J8C37_001435"
     CDS             6131..7390
                     /locus_tag="J8C37_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ5304884.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            7400..8272
                     /locus_tag="J8C37_001436"
     CDS             7400..8272
                     /locus_tag="J8C37_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5304885.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            8284..9114
                     /locus_tag="J8C37_001437"
     CDS             8284..9114
                     /locus_tag="J8C37_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5304886.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            9154..11337
                     /locus_tag="J8C37_001438"
     CDS             9154..11337
                     /locus_tag="J8C37_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAZ5304887.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            11398..12618
                     /locus_tag="J8C37_001439"
     CDS             11398..12618
                     /locus_tag="J8C37_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAZ5304888.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            12615..14075
                     /gene="gtfA"
                     /locus_tag="J8C37_001440"
     CDS             12615..14075
                     /gene="gtfA"
                     /locus_tag="J8C37_001440"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAZ5304889.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            14152..15258
                     /locus_tag="J8C37_001441"
                     /pseudo
     CDS             14152..15258
                     /locus_tag="J8C37_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            15580..15789
                     /locus_tag="J8C37_001442"
     CDS             15580..15789
                     /locus_tag="J8C37_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ5304890.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            15782..15931
                     /locus_tag="J8C37_001443"
     CDS             15782..15931
                     /locus_tag="J8C37_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAZ5304891.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            15966..16750
                     /locus_tag="J8C37_001444"
                     /pseudo
     CDS             15966..16750
                     /locus_tag="J8C37_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(17034..18104)
                     /gene="xerS"
                     /locus_tag="J8C37_001445"
     CDS             complement(17034..18104)
                     /gene="xerS"
                     /locus_tag="J8C37_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAZ5304892.1"
                     /translation="MYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKNYKLFY
                     EWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLS
                     TSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKK
                     LFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSEVININ
                     LDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIY
                     QGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSS
                     TNTTTLYTHIDNAATIDALNAL"
BASE COUNT         6036 a         2810 c         3539 g         5729 t
ORIGIN      
        1 ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
       61 aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt caaacattct
      121 atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg
      181 aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc
      241 gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg
      301 tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc
      361 agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc
      421 ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
      481 gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga
      541 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
      601 gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag
      661 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
      721 ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca
      781 tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg
      841 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata
      901 gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag
      961 cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     1021 cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa taagatggat
     1081 acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat
     1141 tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc
     1201 tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct
     1261 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     1321 ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     1381 cagaaggcgt caccaacact agatattata gatccttaaa aaagtaaaca tccaaaaaag
     1441 ggtgtttact tttttgttta ctaatatttt tagtaaaaaa tctcgacaaa acattaagta
     1501 aacagttatg gtgttgttaa caaaaagaaa acgcattcaa aacaggagga attaagatga
     1561 aacatttcaa aaaaatagct ttaacaggga tagctgtttt aactgggaca ttactcgcag
     1621 catgtggtgg gggagatagt aattctaaag acgggcaagt aactttaagc tatgcgagtt
     1681 gagatactga ccaagctcca ggactaagaa aaatgttaga tgaatttgaa aaacaaaatc
     1741 ctgatatcaa agtagaaatg gagacaacgc cttgggatca atattgggtt aaattagaag
     1801 ctgcaacaac gggaggtaat atgccagatg tagtaaccat gcactctagc gaatcctata
     1861 aatatatgtc acagaatgct ttgatgggac ttgatgatgt tatcaaagaa aatgatattg
     1921 acatgggaaa ctttactgaa ggaattgctg atttctatac gcttgaagac gaactatacg
     1981 caatccctaa agatgcttct gtagtggggc tatggtacaa caaagaatta tttgataatg
     2041 caggtgtcga ttatccagat gaaacatgga catgggaaac actcagacaa gctgcaattg
     2101 atttaacaga tgaatcaaaa ggcatctatg gttttgcatc acatattgga acagagactg
     2161 gctactggcc atttatttat caaaatgggg gagaagtatt tgccgatgac aatacaaaat
     2221 caggtttgaa taccccagaa gcacaagaag ctttagcttt ttatcgagat attgcacttg
     2281 ttgatcatgc ttcaccaaca attacagaat tacaagatcc tgataagtta agtcgttttc
     2341 aatcaggtag attagcaatg atgattgaag gaaactggtt tgcaccggga tttttgaaaa
     2401 atgactatct agttgaaaat gttgcttctg ccgtgttacc taagggaaaa gttaatgcaa
     2461 caattacaaa tggacttggt tgggctgcca gtgcgagtac aaaataccca gaagaagtga
     2521 aaaaactaat ggcattttta gctagtgagg acgccaataa aatccaagcg gaaacggggg
     2581 catcaatccc agcactaaaa ggctttgaag acgagtgggc tgcttcttca gatacaattg
     2641 atttgagtgc ttttggaaaa atgttggagt atggagtccc tcgtccatac aataaagatg
     2701 gattacgagc tgaacaagca gaagttcaaa ttattaaccg tattctgtct ggtgaagtaa
     2761 ctgttgaaga tggaaccgct caaattgaaa aagaagtcaa tgcgatattg agcggaaatt
     2821 aaattagtta attatacaat atacaggcga aaggattcta tttcgtctgt atattaatgg
     2881 atgaatacca aaaaggggga tattatgaaa aatcgaattt ttggaaaata tcgtaaagat
     2941 caagtgatat ggggctatat ttttattttt ccaacggcat taggattaat tttattaaat
     3001 atatggccag caattcaaac agcaatttta agttttcaaa aaacggtagg gttcggtgat
     3061 actgaatggg tcggattaag aaattaccaa aaaatgtttg cagacggtga agtttttcag
     3121 tctttattaa acacattaat ttatgcagtg gtttcgatac ctagtattgt tgtattatcc
     3181 ttgttggtgg cagttttaat gaatagaaaa gttaaaggat tgtcgattta tcggacaatt
     3241 tattttctac cagtcgttgc tgcaccatcc gcagtcgcaa tgatttggcg ctggatgttc
     3301 aataatgatt atggactgat taataacatg ttggctaaaa taggtctcga tggaccagca
     3361 tggttaacgg atccatcgat tgcgatttat tctattatca ttgtaggagt ttggagtgct
     3421 attggttata acatggtgtt attactagcc ggcttataag aaattcctaa agattattat
     3481 gaagcagcgg aaattgatgg tgctagtcca atccagcagt ttttcaacat tacgttacca
     3541 ttagttacac caacattatt ttttgtagtt gtaacaacag ttatcaatgc tttccaagta
     3601 tttgatgtta tttttatgat gattactcct aatagtaccg ccatgtttaa atcacaatca
     3661 ttagcctatt tattctataa acattcattt attttaaatg acaagggata tggttcggca
     3721 atcgttatgt tccttttagt gattatatta attattactg caattcaaat gaagttgcag
     3781 aaaaagtggg taaactatta aggaggctat caaattatga aaaataaatt tttttcaaat
     3841 gcgccaatcc atatcatgct tattttaggt ggtattgcaa tggcattacc ttttatttgg
     3901 atgattttaa cttcagggaa aacgttggtt gaatctaccc aaattccacc tacaattttc
     3961 cctgagaaat ttcagctaag taactatcaa caagtacttg atttattacc attcatggct
     4021 ttttatatca atacattttt aatggttgtt ggacgagtga ttggctcggt tttattttca
     4081 gcaatggctg catatgcttg tgcgcgtcta agttttccag ggaaaaatat ccttttcagc
     4141 ttagtactta ttcaaatgat gattcccggt tccattttta ttactccaca gtatttatta
     4201 gcacaaaaat tgggtatttt aaatacaatt ccagcattaa tattaccagg ggtagttagt
     4261 gcgtttggta cgtttttatt aagacaattt ttcttaggtt tacctgatga attggaggaa
     4321 gcggcctatt tagaaggtgc caatatttgg acgatctttt tcaaaattat gttgccgctt
     4381 gctaaatcag gattggttgc acttggaata ttcacagcgt tgtttgcatt taaagattta
     4441 atgtggccat tgattgtcaa tacttcaatt gataagatgc ctttagctcc gggattagca
     4501 aacttacaag gacaatatgc cacgaacttc ccacaattaa tggcaggttc gttattagca
     4561 atttggccaa tagttgtgat attcatcgtt ttccaaagaa aattcattga aggaattgct
     4621 acaacaggta gtaaattata aaactattaa ttagtgattg gagagtaagt acatgacaaa
     4681 aattacattt attggtgcag gaagtactat ttttgcaaaa aacgtattag gcgatgcgat
     4741 gttaactcca agtctacaag atgcacatat tgcattgtac gatattgatg aaaaacgact
     4801 aaaagaatca aatttaatgc ttaaaaatat taataaaaat tcgaatgaaa atcgtgctga
     4861 tattgtttct tattatgatc gcaaagaagc gttaagagag gctgattttg ttatcaatgc
     4921 tattcaagtg ggtggttata aaccttcaac agtgattgat tttgatattc ctaaaaaata
     4981 tgggttgcga caaactattg gtgacaccat ggggattggt gggattttta gaaccctacg
     5041 gacactacca gtaatgttag actttgctaa agaaatggaa gaagtttgtc caaatgcttg
     5101 gttattgaac tatacaaatc cgatggcgat gttaaccatg ggtgttttaa aagcgacgag
     5161 tattaagact gttggtttat gtcatagcgt tcaagtatgc gtaccagaat tgtttgagca
     5221 tttaggaata gccgatcaat atgacttaaa tgactttcaa tggaaaattg caggcattaa
     5281 tcatatggca ttcttgttgg aaattacaaa aaatggggaa gatttctatc caacaattcg
     5341 tcagttagct gcagaaaaac caaatccaca tccagattct gtacgttttg aattgttgaa
     5401 acgttttggt tactatatta cagaatcaag tgaacacaat gcagaatacc atccatactt
     5461 tattaaatca aaatacccag aattgattga cgaattaggg attccgttgg atgaatactt
     5521 acgtcgttgt gaacaacaaa ttaatgattg ggaaagcatg cgagaggaca ttgtaaataa
     5581 tggggagtta acacatacga gaagccgtga atatgcttca tatatcatgg attctattac
     5641 gacaggaact ccgtcagtga ttgcaggaaa tgtcttaaat aaaggcttaa tcacaaactt
     5701 accgacagat gtttgtgtgg aagtaccttg tttagtcgat aaaaatggcg tgcaaccaac
     5761 ctatgtcggt gaattgccaa cacaattagc tgctttaaat cggacgaata tcaatgttca
     5821 agaactgaca gtagaagccg ctttaacact agataaatca aaaatttatc aagcagcgta
     5881 tttagatcca catctatcat ctgaattgtc tttaacagat atcaaaaatt tagtggatga
     5941 tttaatcgaa gcacatgggg actatttacc tgaatataat taaaaagaag ctgaaagagt
     6001 aaggaatttt caattcctta ctcttttctt agttaaattt agtaaaaata ttgacaaaac
     6061 acttagtaaa cagttacatt agtgttaaca aaaagaaaac gcattcaaat tgtataagga
     6121 ggagcataaa atgaagtggt ctaaaaaaat tattttagct tgtatagcac taggagcaac
     6181 gatctctttg actgcttgtg gaaaaaatca ggaagatgca agcggtaagg tagaaataga
     6241 gttttttaat caaaaaaaag aaatgactca aacgattcag gagattgcta aagattttga
     6301 agcaaaaaac cctgatattc atgtaaaagt tgtggatgta ccgaatgctg gtgaagtaat
     6361 taaaacacgt atgcttgcag gcgatgtacc agatgtcatt aatctttatc ctcaaagtat
     6421 agaattgaaa gaatgggcga aagctggtta tttagaggac ttaaccgaag aaccgtattt
     6481 agagaatatc aaaaatggat atgcacaacg ttttgctatt gaggatcgtg tatatagcat
     6541 accattaacc gctaatgtct atggttttta ctataacaaa actgcttttg aggaaatggg
     6601 gattcaagca ccagaaactt gggcagaatt tgaaaaaata gtagctgaga ttaaagatca
     6661 aaacaaagta ccgtttgcga ttgcaggagc agaaggctgg actttaaatg gttatcacca
     6721 actagctata gcgactgctg ctggtggtga agaggaagcg aacaatgttt ggcgtttctc
     6781 agaagttaat ggaattaatg ctgaaagcaa agaaatgcaa aaagatttta atcgattaga
     6841 tttgttaaga gaaccaaaag ctttgcaaaa caactggcaa ggtgccggtt ataacgatac
     6901 agtagttact tttacaaaag gtgatgcgtt gattatgccg aatggttcat gggcaatgcc
     6961 gatgattcat tcacaaaatc cagattttga agtaggaact tttccttttc cagcagatga
     7021 agcaggtcaa agtctaacga ttggcgcagg agacctagcc ttatcagttt ctgcaacttc
     7081 tcaacataaa gaagaagcca aacgttttgt agaatatatg acaacacctg aagcgatgca
     7141 gaaatattat gatgtagatg gtgcaccgtg tgctgttgag ggcgttatcg agaatactca
     7201 ggattcacca ttaagtggtt taactgaact tgcttttact gatcgtcatt tagtttggtt
     7261 agccaaagat tggaatagtg aaaatgattt ttatacctta acaacaaatt atttacacaa
     7321 tggcaatcaa aaagccatga ttaacgcatt aaatgctttc tttaatccaa tgaaagctga
     7381 cggaaggtag gaaaataaaa tgaaaataaa agaaatttta aatcgttact gggcaatcat
     7441 ttttgtagca attccaatta ttttgcagat gatttttttc tactttccaa tggttcaagg
     7501 tgctttttac agtttaacta attggactgg tctgacttat aattttgatt ttgtgggttt
     7561 gaacaattat aagattcttt tttctgatcc gaagtttatc aaatcgattg gcttcacttt
     7621 aattgtgacg ctttgcatga ttgctggaca aattggtttt ggtattttga ttgcgcgggc
     7681 gttgaacgca aatattaaag ggaaaacatt ctttagagca tggttctttt ttccagcggt
     7741 cttatcgggc gtggttgttt ctttagtatt taaacaaatt tttaactatg gtttaccaaa
     7801 aattggtagt atgttaggca ttgaattttt acaaaccagt cttttaggaa caacatgggg
     7861 agcgattttt gcagtaattt ttgttttact ttggcaagga attgccatgc cagtcatcat
     7921 tttcttagcg ggttttcaaa gtattccaca agaaattatt gaagctgcta gaatggatgg
     7981 ggcgaatgat aagcaaattt tttggaatat tgaaatcacc tacttattac caagtatttc
     8041 aatggtattt attatggcat taaaaggtgg cttaacagca tttgatcaaa tttttgcttt
     8101 aactggtggt ggaccaagcg atgccacaac atctttgggg ttacttgttt ataattatgc
     8161 ttttaaaaac aatcagtttg gttatgcgaa tgctattgct ttagtcttat ttatcttaat
     8221 tgggattgtt tcactggcac aattaaaatt atcgaaaaaa tttgaaatct aggaggtttc
     8281 ccaatgaaaa catcaaaaga aaaaaatatt tctaaatact tgcttttatt attagggggc
     8341 ttcatgattt tagttccctt agtttcaacg ctattcggtt catttaaaac aacacccgat
     8401 attatgaata atttctttcg ttttcctaat ccagcgactt tggaaaattt ccgaagattg
     8461 ttagcggatg gaattggtca ttacttttgg aattcggcaa tcatcacaat cattgcagta
     8521 gttgctgtga cagcaattat tcctatggct gcttattcac tagcaagaaa aatgactcgt
     8581 gggaaagcat acggattgat gtatttattt ttaattttag gaatttttgt tcctttccaa
     8641 gtaattatga ttccgattac cgttatgatg agtaaaattg gtttagcaaa tatgtgggga
     8701 ttaatcttac tctatctcac ttatgccgta ccacagacac tatttttata tgttggtttt
     8761 attaaactaa atgtgcctat cagtctggat gaggctgctg aaattgatgg ggctagtcac
     8821 tttacaactt atttcaaaat tgttttccca atgttaaaac caatgcatgc gacgactatt
     8881 attcttaatg ctctatggtt ttggaatgat ttcatgctac cattattaat tttaaatcgt
     8941 gattctaata tgtggacact accattgttt caatataatt atacgggaca atattttaac
     9001 gactatggtc caagttttgc ttcctataca gtaggaattg tcacgattac actagtttat
     9061 cttgctttcc aaagaagtat tatagctgga atgagcaatg gagcagtgaa ataaccaata
     9121 aattattctc attataaagg agttcataaa cttatgatta tatttgacaa tcacacacaa
     9181 acatttcatt tacaaaataa aaaaattagc tatctattag gaatcgaaga aaataattat
     9241 ttaacgcatc tttattttgg aaaaaagatt acgcactatt ctggtgggta tcgctacccc
     9301 cgaacagatc gttccttctc acccaacaat gcaaatgcgc aagatcgttt attatcttta
     9361 gacacattac cattagaatt tccaggatat ggacatggtg attttagaga accggcgcat
     9421 aatattaaat taggaaacgg tagtcgtgta aatgattttc gttatgatag ctacgaaatt
     9481 caaaaaggaa agcccagctt agcgggattg ccagccttat atacggaaga tgatgatgaa
     9541 gctgaaacgc tgattattca attgaaagac gcgattagtg gattgacttt acagttgttc
     9601 tacacaattt atcaaaatca cagtgtaatt attcgttcta cgcaattagt aaataatagt
     9661 gatgaaacag tggagattaa ccgcttagct agtcaatcaa ttgattttcc aaatcgtcca
     9721 ctcgagttaa ttcatttaaa tggtacctgg gcaagagaac gtcaaatgac tagagaaaaa
     9781 attcatacag ggataaaagt attagatagt aaacgcggat caagcagcca tcaacaaaat
     9841 ccgtttgttg ctttagtaga tgccaaaaca gatgaatttc aaggagaggc atatggattt
     9901 aatttagttt atagcggtaa tcacgaaacg gtgattcaaa aagatccatt tgaccaaaca
     9961 cgggtcatta tgggaattaa ttcatttaat tttggttggc aactaaaaac tggcgactca
    10021 tttcaaacgc cagaagtcgt gatggtttat agtaatgaag gtctgaatac gttatcacag
    10081 acgtatcatg aattgtataa tcaccgttta attcgtggaa actatcgttt aaaagaacgt
    10141 cccacgttaa tcaacaactg ggaagcaacg tattttgatt ttaacgaaga aaaaatcatg
    10201 aatattgtcg atgatgcgaa gaacttgggc attgaaatgt ttgttttaga tgatggttgg
    10261 tttggcggtc gtgaaaatga cttcacttca ttaggtgatt gggttgaaac agaaggcaaa
    10321 cttgcttgtg gtttggaaaa tttagccaag caagtgcatg ataacgggat gaaatttggg
    10381 atttggtttg aaccggaaat gatttctgaa gatagtgaac tatttcgagc acatccagat
    10441 tgggcattgg gtattccagg tcgtgaccgc tcaattagtc gaaatcaata tgttcttgat
    10501 ttttcaagac aagatgttcg tgataatatc tatcaacaaa tgacggcgat ttttgatcgt
    10561 gtaccgattg attatgtgaa gtgggatatg aatcgaaata tgacagaagt ctattctgtc
    10621 ttgttggact caacgatgca aggtgaagtg gctcatcgtt atatgttagg gttatatgaa
    10681 tttttagaaa agttaacaac gaattatcca actattttat ttgaaagttg ttcgggtgga
    10741 ggcggtcgtt ttgatgcagg tttcttgtat tacatgccac aaacttggac gagcgacaat
    10801 accgatgccg tggctcgtct gaaaattcaa tatggaacca gcttagtgta tccaatttca
    10861 agtatgggtg cgcatgtttc agcagtccca aatcatcaaa caggaagaga aacgagttta
    10921 actattcgtg gcaatgctgc gatggctggt gtttttggtt atgagctaga tctaacgcaa
    10981 ttaacttctg aagaaaaagc agaaattaaa gaacaagttg ccttttataa aaagcatcgc
    11041 caacttttgc aatatggaac tttttatcgc ttggaaagtc cgtttgaagg caatgatact
    11101 gcatggatgt ttgtctcaaa agaccaaaca gaagccatgg tcttttattt ccgagtatta
    11161 gcagaagctg cgcatccatt agtcacattg aaattagcag gattagcaga agaagcaact
    11221 tatcaaatgg aagatgtaac atttgctggc gatgaattga tgaatctcgg tttttatatt
    11281 gactcagaac tacatggtga ttatgcgaca caacgctttt atttgaaaca gatatagtag
    11341 cttgaattta caatataagt gcaacaccaa aagagactca ttccaaacga agaaaatatg
    11401 ttttatttac acgcatttac taaaaagcag cctgatttga attgggaaaa tgaagaagta
    11461 cgtgaagaaa tctatgcaat gattaactat tggttagata aaggactagg cggatttaga
    11521 atcgatgcga tattaaacat caaaaaaaaa ctagagtatg gcatctttga atctgatgga
    11581 gaagatgggt tagccttcat tgggccctgg attcttaatc agtcgggcat tgaagtgtgg
    11641 ttaaaagaaa tgagagagcg gacatttaaa ccacataata gtatgacggt agcagaagcc
    11701 gatgttccta atgaacgttt agatgaatat attggtgaag atggctatta ttcgatggtt
    11761 tttgatttta gttatacgga tattgatgta cctgaaactg gtgaatggtt taaagatagc
    11821 caatggacat ggacggatat gcgtaccaat atttttacga atcaattagt tacgcaagat
    11881 aaaggttggg gcgccttata tttagaaaac cacgatcaac cacgttcaat taataaatat
    11941 attccagaag aatggatcaa tgattatagt aaaaaaatgt tggcgacgct ttttatgtta
    12001 ctaagaggta ccccttttat ttatcaagga caagaactag gaatgaccaa catagaaatg
    12061 gcatcattag aagattttga tgatgtagct acgcattctc agtataaacg ggcattagaa
    12121 tatggattgg caccagagca agcattgaaa gctgtcaata aacgaagtcg ggataattct
    12181 cgcacaccta tgcaatggaa tacgcaaaaa aatgccagat tctcaacttc tgataatgta
    12241 tggttaaaag taaatcctaa ttatccagaa ctcaatgcag aagctcagtt aaccaatcaa
    12301 cagtcggtat tcaattatta tcatcaatta attaaattaa gaaaaaatag tgtatacaaa
    12361 gacgtgctaa tctacggaca atttctacca gtgaaagata aaaatgaaca tattttgctt
    12421 tatgaacgcc aattagaaga taaaatcgta ttagttatct taaacgttac agcaacagaa
    12481 caagaatatt atgtagatga acagtaccaa caagtattaa tcaataatta tgaagatgtt
    12541 ttattagatc aaagtcgaaa attgcgttta cgaccgtttg aaagtattgt attagctaat
    12601 tatggaggac aattatgaaa attaaaaacg aagcaatgct catcacctat gcagatagtt
    12661 taggaaaaaa catgggcgaa ttaaaacaag tcttagacaa acatttacaa ggtgttattg
    12721 gtggtgtaca tctgttgcct ttttttccat cgacaggtga tcgaggcttt gcgccgagtg
    12781 attatacgac agttgatcca gctcttggaa catgggaaga tgtggaagca ttaggtgaag
    12841 attattattt gatgtttgat tttatgatta atcacatttc tcgtgaatca aaatttttcc
    12901 aagatttcaa aaaaaaccat gagcagtcac catacaaaga aatgtttatc cgtattcatg
    12961 aatttttccc agaaaatcga ccaacacaag aagatattga tttaatctac aagcgtaaag
    13021 acaaagcgcc gtttcaaacg gtagaatttg cggatggtac gacagaagag gtttggaata
    13081 cctttgggga agaacaaatt gatttagatg tgaccaaaga agttgtgaaa gaatttattc
    13141 gagaaaccat caaagatatg gcgaaccatg gctgttcttt aattcgatta gatgcgtttg
    13201 cttatgcgat taaaaaatta gataccaatg atttcttcgt ggaaccagat atttgggaac
    13261 ttttagacga agtgcgtcaa gaagcagcga aatatgacgt cgaattatta ccagaaatcc
    13321 atgagcatta ttccattcaa atgaaaattg ccaatcatga ttattatatc tatgacttcg
    13381 ccttgccaat ggtcactctg tattcacttt atagtggaaa atcagaacgt ttagccaatt
    13441 ggttgaaaat gagtccgatg aagcagttta caacattgga tacgcatgat ggaattggtg
    13501 ttgtggacgc tcgtgattta ttaacagatg aagaattgga ttacacctct gaggaattgt
    13561 ataaagtcgg ggcgaatgtg aagaaggtct attcaagtgc caattacaat aatttagata
    13621 tttatcaaat taacagcacc tattatagtg cattaggcga taatgaccgt agctatctgt
    13681 tagcacgtgc gattcaagta tttgcgccag ggattccaca aatttattac gttgggttat
    13741 tagcaggaaa aaatgatatt gaactgttgg aagcaacgaa agaagggcgt aatattaatc
    13801 gtcattacta tgatttagcc gaaattgaag aagaagtgca acggccagtg attcaacaat
    13861 tatttgcact tttaaaattt agaaatgaat ctgcggcgtt tgatttggat ggaacgattg
    13921 aagtagcaac gccatcagaa tcagaaatag tgattacacg taaaaataaa aacggtagtg
    13981 atagtgcaac attaacggct aacttggata cgaaagaatt tatcattgag tctaaagata
    14041 taaaaatttt cactagtgtt gcattaaaat tttaacctat tccatagaac aaaaaatcct
    14101 ttatactagc acttggatga cttatccaag accaataaaa aggactcata catggataag
    14161 tataaaacaa atacagcatt taataaatgg ttttctgcca taaaattagg aaaactttct
    14221 gtcaactccc aaaaatgtat ttttgattac aattcgtatc gtaaaaaact gtcttttgaa
    14281 gctgtgctca agctctttct ctacgcagtt aatgaagaaa aagaaagttt gcgtgatttg
    14341 agtacagctc ttatcgaccg atcgctccaa ctagaaaccg atgtgtcatc gattagtcat
    14401 acccaattgt ctcgagcatt taatgcactt gaacctaaag tgttagaaga aatttttcaa
    14461 caactgttag aaaaagttca acatcaaatc aaaccaacca agcgaaatca tctgtatctg
    14521 attgattcca gtacgttttc gcttagttta aaacgtcata agtgggcgac tttccgtcaa
    14581 acaaaatcag gtgtcaagtt acacttaaac tattgttata tggacgatac tacgatgtat
    14641 ccaactgatt ttaccttaac aaatgcccat gaacatgatc tcaatcaact accagtattg
    14701 gtgaatcaac cagaggcaac ttacgtgttt gatcgtgggt attaggactt tgaaaagatg
    14761 gatcagatgc attgggatgg ctatttcttc gtcactcgga ttaaaaaaaa caccaaagtt
    14821 cacgtgatag atactctgga aacatcgcca gaaacagaaa ttctacgaga cgagctggtt
    14881 cgtttaggat ctaaaaccta tctaaccgcc aatttccgac tagtcactgt tcaagataag
    14941 aacggacgtg tgtttcaatt tatcaccaat cgaatggacg tctcttcaaa agaaatctct
    15001 gatatgtatc acgctcgttg gcaaatcgaa ttgtttttca agcacatcaa acaacacatg
    15061 accattaaaa ccttcttttc tcaaagtgaa aaaggcgtgc agaatcaatt gattttaacg
    15121 atgatttccg ctctcctcac gtttctcatc aaattggaga cgaaaacgga aaaatcagtg
    15181 ttccaaatca aacgattctt tcgttattta ctgtttcagc cctttgaatg ttgtctagaa
    15241 aaattaattc ccacatgaga aaaaagaaat ttggctcgtt acttttggtt ttcagttgaa
    15301 taacagataa aaaattttct ggaaaaagtc atcctctgcg tcagcacagt tttttttgac
    15361 ttattttcag aggaaatagt tttcagaata ttctaaaatg ttttatgcaa cactagtgat
    15421 attttttaga attagatttc taatgtgcat tttttgagta ttaaggagca agtatacaaa
    15481 gatttataaa gctgattaaa tttgaataaa aatatgcagg aacgctacta tttttagtag
    15541 tgttcttttt tatatttgaa taaaaaatgt ggtggttaaa tggtggttat aacaaaaaat
    15601 gaattaatga aattaggtta tgggaagtat cagtctgaga gaatcatacg acaggcaaaa
    15661 gcaattatgg tttcaaaaag ctatacctat tacgaaaata gaagattagg actagtacct
    15721 gttgaatctg tagaagaagt tttaggtatg aagatagagg tagaggagga ttttcagaat
    15781 catggttaat tttaaaaaag ataaaaatgg gaaatatcat tttactgcta gtttgggatt
    15841 cgacaaacga acagggaaaa gagtacaaaa aaacgtagag gggttaaaac agttaaagaa
    15901 gaaaaaaagc gtataccgaa cttgtttcta attatggaaa agaagcagaa acttctcaaa
    15961 gtactatgtt gtttgaggag tttttttata aattattcct accttattat caagggaaag
    16021 tagaagaaag aacatataaa aatcggttat ctacaatcaa aaaaactttt cttatttctt
    16081 taaaatggag ttaagagaaa tacgtccaat tgacattcaa aagtggcaaa ataatttatt
    16141 gagcaaatat aaaaattctt atgtaagaaa catgtatggg atttttcaga tgtcattaga
    16201 tcgagcagtt gttttgggac tacttgaaaa aaatcctgca aaagttattg gaaatgtgaa
    16261 gaaaaggaaa actacagttg atttttggac aaaagaagaa tttacaaaag ttatttcaac
    16321 tttttatatt gatgatttgt accaacattt tggttttatt tgtatgtggt tactttttat
    16381 gaccgttatg cgtttgggag aagccactgc attaacttgg aaagatattg attttgaaaa
    16441 taaaaacttg cgtgtgaata aaacactcta ttataagaga gccaaaacct atgagtttgt
    16501 agaacccgaa acggcttcta gtattcgtac aatcgaacta gacgacaata caattacctt
    16561 tttaaaagct tggaaggagc gtcaagtttc aataggaggc atgtcgtttg tactttctta
    16621 taatggaaca cctactcaga aatatacaat ccgtcatatt atgagaagac atgcaacgat
    16681 cgttggtgtt catataatcc gcatacacgg attaaggcat agtcatgctt ctttgcttat
    16741 atcacaataa aaagatgcac ttcgaattaa agaaaggtta aaacatgctg atgtacaaac
    16801 gactttagga acttatggtc atttgtatct aaattcattg agagagataa caaatgatcc
    16861 cacagattat atccaaacta atttttctaa aatcaaaccg acacctattt acgataataa
    16921 aaaataaatg ataaacaaaa gtaggatcac accaaaagaa acacactaga aaaactataa
    16981 tacaggcttt tctagcgtgt tgtttcttat ttccgcctat ttatacatta tactcataaa
    17041 gcatttaatg catcgatcgt agccgcattg tctatatggg tatataaagt tgttgtattg
    17101 gtacttgaat gtcccaattg ttgggcagtt aataccaatg aatttgtctg ttggtagagt
    17161 ctagtagcta atgtatgacg cagtttgtga gggctgactt gaattctaaa gccttttgaa
    17221 tattttgcta ccatccgttc gattgcaata ccggatatac gcttggcttc tccttggtaa
    17281 attgctagaa ataaagcttt ttcgttttct gttactttat atcgttcttt ccgaatagct
    17341 agataattag ctaaatattc cattgcgaat gctgctatat tgacagcatc tcttttattt
    17401 ccttttctag ttactaccac attattctta tctaaagata ggtcatctaa attaatattt
    17461 ataacctcgg agagtctcaa tccagatcct aaaatcagtg cattgatcgc caggtctctt
    17521 tctacatctc gttgataata gatcaaagct cgtttactca aagtattttt atattcattg
    17581 gcaatataat ctagataatt ttgtgtatcc ttatctagaa ataatttttt ctcaatggca
    17641 ttggcacgtg cagctaacgt agttttatct gtgatggttg ctacttgatc cataggatta
    17701 ttgtctaagt atggttttcc tagaggatta ttggaacttt tcgaaagata tttaaataga
    17761 acctttaatg ccgtgatatt tctattgata gtagaagtag atagacctaa gttattatta
    17821 tctttgagct tagtttttgt tgtctctttt tttacatttt cttttgtgcg acgtttcaaa
    17881 tgagatttgt acaagtttag gtcataggca gttaactgtc ttatcgaggt gatagaagta
    17941 tcaggaacga tagactcact cagaagccat tcataaaaga gcttataatt ttttagatat
    18001 tctaaagtgg ttcgaggtga ttgatcttta tcctcacgat cttgaacaaa atcttttata
    18061 taatcgggca tatgaagtat gattgtatct attttttgat atatttctgt attt
//
LOCUS       DADOOS010000051        17394 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.051, whole genome shotgun sequence.
ACCESSION   DADOOS010000051 DADOOS010000000
VERSION     DADOOS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.051"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            224..1525
                     /locus_tag="J8C37_001446"
     CDS             224..1525
                     /locus_tag="J8C37_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAZ5304893.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1594..2193)
                     /locus_tag="J8C37_001447"
     CDS             complement(1594..2193)
                     /locus_tag="J8C37_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5304894.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            2447..3328
                     /locus_tag="J8C37_001448"
     CDS             2447..3328
                     /locus_tag="J8C37_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5304895.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            3341..4951
                     /locus_tag="J8C37_001449"
     CDS             3341..4951
                     /locus_tag="J8C37_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAZ5304896.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(5028..5687)
                     /locus_tag="J8C37_001450"
     CDS             complement(5028..5687)
                     /locus_tag="J8C37_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAZ5304897.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(5970..6635)
                     /locus_tag="J8C37_001451"
     CDS             complement(5970..6635)
                     /locus_tag="J8C37_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304898.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(6782..7387)
                     /gene="dhaL"
                     /locus_tag="J8C37_001452"
     CDS             complement(6782..7387)
                     /gene="dhaL"
                     /locus_tag="J8C37_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAZ5304899.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(7415..8398)
                     /gene="dhaK"
                     /locus_tag="J8C37_001453"
     CDS             complement(7415..8398)
                     /gene="dhaK"
                     /locus_tag="J8C37_001453"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAZ5304900.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(8400..8795)
                     /gene="dhaM"
                     /locus_tag="J8C37_001454"
     CDS             complement(8400..8795)
                     /gene="dhaM"
                     /locus_tag="J8C37_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAZ5304901.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8949..9269
                     /locus_tag="J8C37_001455"
     CDS             8949..9269
                     /locus_tag="J8C37_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAZ5304902.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(9381..10250)
                     /locus_tag="J8C37_001456"
     CDS             complement(9381..10250)
                     /locus_tag="J8C37_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ5304903.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(10346..11620)
                     /locus_tag="J8C37_001457"
     CDS             complement(10346..11620)
                     /locus_tag="J8C37_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ5304904.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(11646..12155)
                     /locus_tag="J8C37_001458"
     CDS             complement(11646..12155)
                     /locus_tag="J8C37_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304905.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(12330..14465)
                     /locus_tag="J8C37_001459"
     CDS             complement(12330..14465)
                     /locus_tag="J8C37_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5304906.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(14548..16203)
                     /locus_tag="J8C37_001460"
     CDS             complement(14548..16203)
                     /locus_tag="J8C37_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ5304907.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(16205..16519)
                     /locus_tag="J8C37_001461"
     CDS             complement(16205..16519)
                     /locus_tag="J8C37_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAZ5304908.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            16689..16949
                     /locus_tag="J8C37_001462"
     CDS             16689..16949
                     /locus_tag="J8C37_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAZ5304909.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            16963..17277
                     /locus_tag="J8C37_001463"
     CDS             16963..17277
                     /locus_tag="J8C37_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAZ5304910.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         4887 a         3556 c         3131 g         5820 t
ORIGIN      
        1 ttgtcaacag aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct
       61 ctaattccga acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt
      121 tctgcaattt atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag
      181 gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa
      241 aattatttta ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt
      301 tgtcatccga tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa
      361 agcaaaagaa ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca
      421 aagctacgat tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac
      481 tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc
      541 tgatcctact cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc
      601 cttccactct caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa
      661 ccgttcgatc gaacaacttt atcaaaatga ttttgtttta gttaatttaa atgatttgat
      721 ccaaaaagac gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa
      781 accattggtt ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt
      841 tcctagccga ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga
      901 taaagaagaa atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca
      961 tcctgatttt tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt
     1021 tttaggttat cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat
     1081 taaagaagct aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca
     1141 tacatggggt cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga
     1201 attgtggcag caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt
     1261 tggtgcagac atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt
     1321 gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca
     1381 attaggtacg gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc
     1441 tgatttagac ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga
     1501 caaagaagca cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg
     1561 acattttatc gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt
     1621 gatcaggact tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt
     1681 agaaattcct tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa
     1741 atccactttt ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc
     1801 ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt
     1861 catcaaatcg atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact
     1921 catggcaatc agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg
     1981 ccctaaatca ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa
     2041 atatttaaaa atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac
     2101 tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt
     2161 tttctcttgt gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact
     2221 tttataatat atatagttca aatttattct gaggtttatt ataactatct attgagaaag
     2281 tatcaagcta gcaattcctt gtttatgaat tcattgacag aaatttataa aaagaatatt
     2341 atgtactaaa ataataaaaa aagttttata aaaaggtata aaaccttttt attaaactat
     2401 ttaatcttgt ttagtatata tttttagaaa aaggagtggg gtttttatgg cagaaatcgt
     2461 caaagtcaca catttgaaaa agagctttgg tcgtttcgaa gctttaaaag atgtctcttt
     2521 caccgtacat tcaggtgaag tcgttggatt tatcggacca aatggttctg gaaaatccac
     2581 gacgattcgg acacttttag ggattataaa aaaagacggc ggaacggcaa cgatttttgg
     2641 gcaagatgta tgggaagata gtttatctat ccatgaacgt ctttcttatg ttccaggcga
     2701 tatctctttg tggggaaatt tgactggagg agcgatcatc gatctcttca tgaagcttca
     2761 tggaaaaggg agtgctcaaa aaaaagatga actgattcaa cgtttctctc ttgatcctaa
     2821 gaaaaaagca aaaaactatt caaaaggaaa tcgccaaaaa gtgggcttga ttgctgcttt
     2881 ggctgttgat tcggatctct atttatttga tgagcctact tctggtttag atcctttgat
     2941 ggaaacgatt ttccaagaag aagttgaaaa attgaaagaa cagggcaaat ccatcctact
     3001 ttcttctcat attctcagtg aagtcgagcg actagcggat aaagtcgtga tcatacgcga
     3061 aggaacagtc gtagaagaag gaacactcga tgaattgcgt catttgactc gttcgaccat
     3121 cactatagaa acagaagcag agaatagtca cttagcacaa ttgaacggtg tccatgactt
     3181 tgtgcaaaaa ggtcacctcg ctcaatttgc tgctgataac gaagctttcg atgtgatttt
     3241 agctgaagcg tccaaattag gtgtgaaaaa atttgaatct attccaccga ctcttgagga
     3301 tttattcatg agacattatg aaggatgagg aggaagcgaa atgactgata aattttcacg
     3361 ctggtccacc cttttttctc aaaaccttag ccgtgactgg aaaaagatcc tgatatggct
     3421 agtcggtgtc actgcttttt ctggtggttt tgtccctgcg tttgaagaat tgacttccgg
     3481 acaaggatta gctggattat acgaaacctt gcaaaacccg gcaatgattg caatggtcgg
     3541 accaactcca gcttcgaatg ctgctgagta tactttagga gcgatgtatg caaatgaaat
     3601 gttgcttttt tgcagtttgt ttgctgctgt gatctctgtt ttacacgtgg tgagccaaac
     3661 gagaaaaaaa gaagaacttg gtctgattga acttattcgt tcctttcgag tcggacgtca
     3721 agcaaactct ttagctgtca tcgctgaaag tattctgatc aatttgcttt tagcaatatc
     3781 gatcagctgt acgatgattt ttttccaagc agattcgatc actgcagaag gtgcttggct
     3841 ttttgggcta tctgtaggta gtatggggat tttggggagt gccgttgcac tgtttgctgc
     3901 acaactgatg ccaagttcag ccagttcgaa tggtctgtct ttagcgtttt taggtggact
     3961 ttaccttcta cgagcaataa cagatgtagc tgattcctcc ctttcgatgt ggaatccttt
     4021 aggctggtct tatttgacct atccatttac aactaacaac tggattcctt tgctttattt
     4081 gtttttgttc agtctaatct tgataatcag ttcatttgtt ttagaggaac atcgagattt
     4141 gaacaccggc tatatccccg aacgtgaagg aaaaccgtat gctaaacgtt ctcttttatc
     4201 cttgcgtggt ttattattct atctaaataa agggatgaca atcagttggc ttatcacttt
     4261 tggtttgatg ggggcggcat acggctctat ctacgggaat atgcaacagt ttttagaaag
     4321 taacgactta atgaaacaga tgtttatcca atctggcgtc tcaattgaaa aaaactttac
     4381 aagcacgatc atggtcgtct tgatttgtct agctgctatt ttaccaattg ctagcatcaa
     4441 tcgtttattt acagaagaaa cccacggacg tatgaatcag ctgttcgcaa caaaaatttc
     4501 taggaaccaa ctttattgga ccaatatgtt actagctatt ctatttgcta tattggcaat
     4561 cctgattagt tctggtgcac tagggctttc tgctttggct tcgatgaagg atgcgaaaga
     4621 catggatcta ttgatgtttc ttaaagccgg gttcaatttc ctgcctgccg ttttagtgtt
     4681 tatcggaatt agtggctttc tattcggatg gcttcctcga atgggtaaac ttgcttatat
     4741 ctacttagct tactcctttg cactcaatta ctttggcgga attttggatt taccagaatg
     4801 gttttccaaa acagctgtcc ctagctgggt tccacggatg ccaaacgatt cttttgatct
     4861 ttccgtgttc cttacgttat catgtatcag ccttgcactg ttcgtccttg gttctattgg
     4921 ttataggcga agagacttac tagaaggata ataaaaaagc atgtacaatt tcatgctagc
     4981 ctccaaatct gggtcaaaaa tccaacattt ggaggtcatt tttttattta caagtcttct
     5041 aactgctctt tatcaatgat ccagacttct cgattctttc tttttacata accattctcc
     5101 tctaataatt taaattttct agataaagtt tcgggggtcg taccgataaa tgcagcaagt
     5161 tctttcattt tcattggcag gaaaaagtga tccttccctg tcgctttagc taagtcaagc
     5221 agatagacag cgactcgttc ttctactttt tccatcatca aaaaagttgt ctgctgctgt
     5281 acttttgtta atttctcaac gttgattgct aaaagtttca gactaagggc gggataatgc
     5341 agtaagagtt ccgagaaatc tttttgtctc aatatgcaaa tttccgtttt ttctagtgct
     5401 tcgccaaata aagactcatt tggaacgcct agcaactggc tctctccctc atatccgccc
     5461 ggctctatta ctcgtaaaag ctgttccttt ccgctagcag ataattgata cactttcata
     5521 ctgccggagg ccacgatcac taattgaggt tctccattcg gcgcaagaat ctgttctcct
     5581 ttttccacat agtgatgttt gacaagtgcg tgtacttttt cttgatcttg ctgtactaag
     5641 tgattgaaca aaggaacaaa tgaaacacat aaatgatcat gactcataat ctctcctcct
     5701 tctaaacagc tatttctaat aaagaaacat aacttccctt attccaactg ataattttct
     5761 tttatcatag cataaacgtc aagcgaacca gataccagca aatagagtgg atttcttttt
     5821 gtcccttgct ttattttcct tcttttgaga ccttaagtaa ttgattccta tcaatttttt
     5881 aaatatttac tttttgaata atcaaaaaca aaccttcact cgcaagccga gagtaaggtt
     5941 tgtttttcgc attgcttatt tcgtgttgtc tattgaaatt tttccgcacc tagcttttga
     6001 tatagtggtg ctagttcttg taaggtttca gcggttattt gctcaacgtg ttcgatttct
     6061 tgcggataga agaatagttt acccatttga aaggttcggt ctctcatcgc ttttttcaac
     6121 tcttcttgtt tctttgtatc ggacaaatag tcagacaaag ctaagtggtc gtcaagttca
     6181 tgttctggtt gcggccatat gtaataatcc ttgatatcga ctgttttgct ccactctttc
     6241 agtatcgcaa gcaactggtt atacgctgca atgctaccat atcgttcttc gcttttataa
     6301 tgttggtaca tcaaatagat ttggtattgt ttttgattca acaaaactgc tagacaggca
     6361 ttctcgtctt gacgagattc gcttcggtac gcgctccaaa aatgactgcg taaattccag
     6421 ccattcgtcc agctttcgat tttaggtttg ctcatgccgt acatatcagg caaaagtgca
     6481 gctgtctgga gctgaatctc tttccatttt tgccagtgtt ctttatattc tttcttgatt
     6541 tgttcaatct tatctgttgg atggctctct ttgagctgtt tgaatgcgta gattggtcga
     6601 tcaaacaact gataatctga ttttccaagt tccattgttt cgcctctttc cgttatcttt
     6661 tgtattctac ctgaattttg cgatatggac aaatagaggt tgtgaaaaag ctgttctgca
     6721 tcaagtcata agaacagcca aacaaaaaac tgcgatgagc atcaccgcag ttttattgaa
     6781 cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta gaagcagctc
     6841 ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct tttttagcta
     6901 ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa ttccctgcct
     6961 tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca gcttctgcct
     7021 ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat ttttcttgag
     7081 aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca gatccgtata
     7141 acggaccaga agccccaccg actttcgata tcatcgccat agagaatact ttgaatgtat
     7201 cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa acccctcttc
     7261 ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg agatattgtt
     7321 tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct tgaacggtta
     7381 gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc tgtacattgc
     7441 tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga gaaagacctt
     7501 gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg acgcctcttt
     7561 cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa ggcgtcccgc
     7621 ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca tctaaaatct
     7681 tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga cgatagcctg
     7741 gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg aatcctggtt
     7801 tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta gttgcctgta
     7861 cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt gctgcatcgc
     7921 ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca gtatacgtac
     7981 tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata tcgtccatag
     8041 cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc ttaacgatca
     8101 gcaaaactcc ttttccacta tccgcttcct taatagccgt ctgaatctga tctggagttg
     8161 gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct acaaacccgg
     8221 catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt tccgctttct
     8281 gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga tagcttttta
     8341 ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt tttttcaatt
     8401 tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg agtatcatct
     8461 gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc atccaccaga
     8521 tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga gttcaatacc
     8581 gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc attgactgct
     8641 tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag agaaaaaatt
     8701 tgcacctctt cggatttttg catctgctca atcatttctt taataccatc tgttactttt
     8761 tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc ttttacatag
     8821 ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat aaatttctaa
     8881 ttttgggaag atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga
     8941 gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata
     9001 aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg
     9061 ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa
     9121 gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca
     9181 ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat
     9241 gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag
     9301 agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct
     9361 accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact
     9421 aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc
     9481 aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt
     9541 ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta
     9601 gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc
     9661 tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca
     9721 gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc
     9781 ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca
     9841 agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct
     9901 gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt
     9961 ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat
    10021 gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt
    10081 tctgtttccg aggcaccgtt ttcagcacca gaagagctta tagttgttga aggttctgtt
    10141 tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca
    10201 gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat ccttactact
    10261 ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct
    10321 gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc
    10381 cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg
    10441 cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc
    10501 ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa
    10561 agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat
    10621 atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc
    10681 agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat
    10741 ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt
    10801 ttctttgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag
    10861 gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg
    10921 tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat
    10981 ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc
    11041 ccacgatact tgagaattca accgtttgag attttgcaat caatttggta cgattttgga
    11101 catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca
    11161 catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt
    11221 tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga
    11281 tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt
    11341 ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca
    11401 tcaaatactc gattgtatct tcgatcacaa tttcttcatt atgggctggc accatgatcg
    11461 tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata
    11521 aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa
    11581 cgagaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg tatgggtgat
    11641 tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa tgtcgtatct
    11701 agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc ttttgttcca
    11761 aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca
    11821 tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct agtcaataca
    11881 aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc
    11941 attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc tggtctcgtt
    12001 aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa aagagccact
    12061 aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc tttcaccaaa
    12121 attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt ttttttgtta
    12181 cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc taaatttttc
    12241 ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt tctttttatc
    12301 agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac gacaatgctt
    12361 ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg
    12421 ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag actcaaagga
    12481 atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg tagcgtttgg
    12541 gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga aaccaaaatc
    12601 tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc gggttctttt
    12661 ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata ggttttgaaa
    12721 agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg atggatcggc
    12781 agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga attaaagatt
    12841 gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc gatcaataga
    12901 cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg aaatgctggt
    12961 acatttacct tttcaacaag taaatgacga atagctaata taatgactgc ttcatcaaca
    13021 gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat tctttcaaac
    13081 aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt tcgatatttt
    13141 ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc agaaatcaat
    13201 ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt ctgcacagca
    13261 ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc ctttttcgtt
    13321 gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat tttttcgatc
    13381 gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa attcggatga
    13441 tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct acgcaaacgt
    13501 tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc ttcaccttct
    13561 atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag tggatttctt
    13621 tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt aggtgataga
    13681 atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata cgcaatacgc
    13741 tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat attttgactt
    13801 ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat agccttcatt
    13861 tcattggtct catcttggcc taagacttct aataatacgt catcttgaat agctacagac
    13921 gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct ttctttaata
    13981 accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat gttattgtaa
    14041 aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc atcatctgtg
    14101 aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa gtctacaacg
    14161 acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg attttctgcg
    14221 tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat catcgaaaaa
    14281 gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc catttctttc
    14341 aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc gtttttttta
    14401 tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg
    14461 accatacaca ttctcctctt tttttaaagt ggtggacccg attagtttac ctcttttaag
    14521 agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt cttcaggtcg
    14581 gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc cagactgctt
    14641 caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga tatattcttg
    14701 ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat gtttggcttc
    14761 cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa tttttccttt
    14821 tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt ctgcaatgtt
    14881 cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg gcggtgcata
    14941 cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa
    15001 aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat aaatctcgcc
    15061 agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat aatagcttgc
    15121 atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt tggctgttct
    15181 ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg ctgttccaat
    15241 accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc taccttgacg
    15301 attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct gttttacaac
    15361 gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt cgtctactag
    15421 gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt tatcaggacg
    15481 aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat ccagtcgcaa
    15541 tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt ctactccttg
    15601 atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg gaagaatatg
    15661 tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt ctgccatttc
    15721 caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt ctaatgcatg
    15781 catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg cttctgttat
    15841 ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg catcgtactg
    15901 ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg gaaagatggc
    15961 tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg attctggcgt
    16021 ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag gcaagccgca
    16081 atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat cttccattaa
    16141 tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa taacgatttt
    16201 catcttattt ccctcctact gttcgatcaa accattgatt catgccacca cgtacattca
    16261 ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc atccctgatt
    16321 gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata ctttgtaatg
    16381 gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa
    16441 gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc gattttattg
    16501 attgaaatag caaattcatt ttctgcacct catttacatt tttataaata cctcatgggg
    16561 tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt tttttcacta
    16621 ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa aatagaggag
    16681 aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt acagagggac
    16741 aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat gtcatcactc
    16801 aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt gcagataatc
    16861 tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct aaactagaac
    16921 aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt cgttttccat
    16981 attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct gataaaagct
    17041 tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg ttatctagac
    17101 tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct
    17161 atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt tgtaccagca
    17221 ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa gccttagtca
    17281 gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt tcgaagaagc
    17341 tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc aagt
//
LOCUS       DADOOS010000052        17345 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.052, whole genome shotgun sequence.
ACCESSION   DADOOS010000052 DADOOS010000000
VERSION     DADOOS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.052"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            2..369
                     /locus_tag="J8C37_001464"
                     /pseudo
     CDS             2..369
                     /locus_tag="J8C37_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            549..1490
                     /locus_tag="J8C37_001465"
     CDS             549..1490
                     /locus_tag="J8C37_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAZ5304911.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="J8C37_001466"
     CDS             1559..2290
                     /locus_tag="J8C37_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAZ5304912.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="J8C37_001467"
     CDS             2434..4614
                     /locus_tag="J8C37_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAZ5304913.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="J8C37_001468"
     CDS             4634..5116
                     /locus_tag="J8C37_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAZ5304914.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="J8C37_001469"
     CDS             5132..5893
                     /locus_tag="J8C37_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAZ5304915.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="J8C37_001470"
     CDS             6024..7358
                     /locus_tag="J8C37_001470"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAZ5304916.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7786..8271
                     /locus_tag="J8C37_001471"
     CDS             7786..8271
                     /locus_tag="J8C37_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAZ5304917.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8264..8947
                     /locus_tag="J8C37_001472"
     CDS             8264..8947
                     /locus_tag="J8C37_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAZ5304918.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9073..11718
                     /gene="polA"
                     /locus_tag="J8C37_001473"
     CDS             9073..11718
                     /gene="polA"
                     /locus_tag="J8C37_001473"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAZ5304919.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11763..12599
                     /gene="mutM"
                     /locus_tag="J8C37_001474"
     CDS             11763..12599
                     /gene="mutM"
                     /locus_tag="J8C37_001474"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAZ5304920.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12563..13192
                     /locus_tag="J8C37_001475"
     CDS             12563..13192
                     /locus_tag="J8C37_001475"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAZ5304921.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13244..13576
                     /locus_tag="J8C37_001476"
     CDS             13244..13576
                     /locus_tag="J8C37_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAZ5304922.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13888..14379
                     /gene="nrdR"
                     /locus_tag="J8C37_001477"
     CDS             13888..14379
                     /gene="nrdR"
                     /locus_tag="J8C37_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAZ5304923.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14402..15775
                     /locus_tag="J8C37_001478"
     CDS             14402..15775
                     /locus_tag="J8C37_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAZ5304924.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15775..16701
                     /gene="dnaI"
                     /locus_tag="J8C37_001479"
     CDS             15775..16701
                     /gene="dnaI"
                     /locus_tag="J8C37_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAZ5304925.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16830..17270
                     /locus_tag="J8C37_001480"
     CDS             16830..17270
                     /locus_tag="J8C37_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAZ5304926.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3821 g         4584 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttcccgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt tttcttggct gttcttatta cttgatgcag ggcagctttt tcacgacctc
     7561 agataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa tgaaaaatat
     7621 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7681 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7741 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7801 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7861 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7921 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7981 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     8041 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     8101 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8161 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8221 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8281 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8341 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8401 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8461 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8521 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8581 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8641 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8701 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8761 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8821 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8881 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8941 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     9001 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     9061 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9121 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9181 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9241 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9301 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9361 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9421 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9481 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9541 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9601 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9661 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9721 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9781 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9841 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9901 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9961 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
    10021 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
    10081 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10141 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10201 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10261 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10321 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10381 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10441 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagataa aaaccaagaa
    10501 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10561 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10621 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10681 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10741 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10801 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10861 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10921 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10981 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    11041 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    11101 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11161 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11221 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11281 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11341 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11401 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11461 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11521 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11581 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11641 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11701 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11761 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11821 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11881 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11941 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    12001 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    12061 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12121 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12181 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12241 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12301 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12361 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12421 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12481 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12541 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12601 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12661 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12721 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12781 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12841 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12901 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12961 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    13021 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    13081 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13141 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13201 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13261 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13321 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13381 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13441 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13501 tgaccacatt tccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13561 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13621 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13681 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13741 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13801 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13861 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13921 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13981 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    14041 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    14101 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14161 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14221 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14281 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14341 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14401 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14461 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14521 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14581 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14641 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14701 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14761 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14821 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14881 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14941 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    15001 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    15061 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15121 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15181 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15241 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15301 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15361 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15421 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15481 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15541 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15601 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15661 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15721 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15781 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15841 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15901 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15961 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    16021 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    16081 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16141 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16201 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16261 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16321 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16381 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16441 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16501 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16561 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16621 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16681 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16741 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16801 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16861 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16921 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16981 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    17041 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    17101 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17161 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17221 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17281 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17341 atggg
//
LOCUS       DADOOS010000053        17234 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.053, whole genome shotgun sequence.
ACCESSION   DADOOS010000053 DADOOS010000000
VERSION     DADOOS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.053"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            29..376
                     /locus_tag="J8C37_001481"
     CDS             29..376
                     /locus_tag="J8C37_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304927.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
     gene            471..1355
                     /locus_tag="J8C37_001482"
     CDS             471..1355
                     /locus_tag="J8C37_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAZ5304928.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1579..4746
                     /locus_tag="J8C37_001483"
     CDS             1579..4746
                     /locus_tag="J8C37_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304929.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4766..5311
                     /locus_tag="J8C37_001484"
     CDS             4766..5311
                     /locus_tag="J8C37_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAZ5304930.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5339..5794
                     /locus_tag="J8C37_001485"
     CDS             5339..5794
                     /locus_tag="J8C37_001485"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAZ5304931.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5791..6549
                     /locus_tag="J8C37_001486"
     CDS             5791..6549
                     /locus_tag="J8C37_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAZ5304932.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6635..7375)
                     /locus_tag="J8C37_001487"
     CDS             complement(6635..7375)
                     /locus_tag="J8C37_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304933.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7436..8689
                     /locus_tag="J8C37_001488"
     CDS             7436..8689
                     /locus_tag="J8C37_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAZ5304934.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9011..9238
                     /locus_tag="J8C37_001489"
     CDS             9011..9238
                     /locus_tag="J8C37_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAZ5304935.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9361..9687
                     /locus_tag="J8C37_001490"
     CDS             9361..9687
                     /locus_tag="J8C37_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAZ5304936.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9755..10468
                     /locus_tag="J8C37_001491"
     CDS             9755..10468
                     /locus_tag="J8C37_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAZ5304937.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10778..11629
                     /locus_tag="J8C37_001492"
     CDS             10778..11629
                     /locus_tag="J8C37_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5304938.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11750..13615
                     /locus_tag="J8C37_001493"
     CDS             11750..13615
                     /locus_tag="J8C37_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5304939.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13663..15072
                     /locus_tag="J8C37_001494"
     CDS             13663..15072
                     /locus_tag="J8C37_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAZ5304940.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15180..15881
                     /locus_tag="J8C37_001495"
     CDS             15180..15881
                     /locus_tag="J8C37_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5304941.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16040..16519
                     /locus_tag="J8C37_001496"
                     /pseudo
     CDS             16040..16519
                     /locus_tag="J8C37_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5755 a         2899 c         3706 g         4874 t
ORIGIN      
        1 tctcacaaat aaaaaggcag gaagtacaat ggatcatttg tatataaaat atattcatca
       61 attttccgtt ttggtgttag gcacactaat tatagcgggg attgtcgcat ttattattca
      121 aggccgtgac atattaacag tacatcaaga gtttttcagt tatgcatttg gtattcctct
      181 gatcgctata aggtctgata acaaaaagtg gaagaaacgc tattacattc tcttgctatt
      241 tttgtgtttt tatctgccac ctaagatttt tactttatta ggtatacaat gcctatcatt
      301 ttcttttatt tcctatatca ttgttttaat tttcctcagt ttcatagtat ggatgtttta
      361 tcttgtcaaa aaataaatga atgtttcatg aacagtactt catcacagcc taattttttt
      421 ctttatgcta tcgttatatt aaagaaatca atcagaaggg aaagaaagtg atgaaaaaag
      481 ttttattagt ggtaaacccc agttcaggcg gtgaacaagc aaaggaattt gagcaattag
      541 cgatagcaaa attggaatcg gtgtttgatg aagtggtcgt cttgcataca aaaaaagcag
      601 gggatgcaaa aaactttact cgcgaagcgg ctacggaagg gtatcatagt gtgtttgtca
      661 tgggcggaga tggaacagtc aacgaaggaa tcagtggaat cgctgaacaa gaacatcggc
      721 caaatttcgg atttttccca ttgggtacgg tgaatgactt ggctcgagca ttagggatac
      781 cgctagaacc tgaggaagcg atcaatcatt tttcgattga atcagtcaag ccattggata
      841 ttggaaaaat aaatgacgac tattttatga atgtcgtcgc aattggctct atccctgaat
      901 caatcaatga tgtagatgcg gagaagaaga cgaaatttgg aaaactagct tatttcatgt
      961 caggaatcaa acagttagct tccacacaat cctactcgtt tcacgtagaa gttgatggaa
     1021 agaaggaaga aattgagagc agtaccttgc tgataggact aactaactca gtcggcggtt
     1081 ttgaaacatt acttccaaat gcaaaagtgg atgatggtaa gctccatttg gtttatttaa
     1141 aagattcttc tttgatcgat acgcttaaaa cattgccaga cctattaaaa ggagtagatg
     1201 aatctacgaa aaatctagcc taccttactt gtgagcaatt agttgtgtca ttagctgatg
     1261 aggaagaatt gacgacgaat gttgatggag atgaagggga taaattacct gtgacaatca
     1321 aagtattgcc ctcacattta caggtttatt gttaaaatgg aggttgtgac cgaggagttg
     1381 gcgggagaac accgcttgtt cgggataagg gactgtgaca agacttttgt cacagccttt
     1441 tttttgtaca aaaaagaaat ttacacctaa tagaaaaatt tttccataaa aatacatatt
     1501 ttaaatatga aagtacaatt ggaaatagat taaaacgata aaataaaatt gtcaaaacgt
     1561 cagagaggat ggtggagaat gaatcaatgt gtaaaaaagc tgattgcgtc gattttgttt
     1621 gtcttccctc tcttgatggt gacatccgtt tttgccgatg tagagaaaac aaacgaatca
     1681 acgcaatcag agaatcaaag ttttgacaag actacttttt cagaagaatc gattacttct
     1741 gaaactacag aatctcaaag tgaggcttct actaataaaa ggcaagaaga aaataagaca
     1801 agtgagacct cagaagtagc tgaacaaacg tctgatagca gtgaagcggc actggcaaga
     1861 gctgtttcag cggatgggta cagcgaagct gccacaacag aagaactggc gcagcttctc
     1921 gcagacgaat cagtaactaa gatccgactg attcagcctt tgactttaga cagggaatta
     1981 gagatcaaac gagatatcgt tattgatttt ggtggattcg ctcacaattt cggcacacac
     2041 catatttata tcaatgagat acctcagtcg atcgagttcc agaattttaa aggaacagct
     2101 gcacatccag gtggattgac accaaatatc gatggaaatg ccattatcat tgcttatatg
     2161 accgattttt ggggaagcag atatcatttt acgggagaaa taaaatttac cgggacactt
     2221 gatttgtatg atgggtccaa attaggactg atttacgctc ctcgtgcaac tgtcacatta
     2281 gatggtgttt ccggtgtgtt ggatgtccaa ccagtgaagg aaggactgaa tgccgctccg
     2341 ggtaaagcct atttttgtag aagctatcaa ttgaatgtga tcaatggtag tcaactttat
     2401 ggaccgtatc tagggaaatt ctacggtttt ctagattcag gagatgagac tggtagtgga
     2461 aaaacagcgc caggaatcca tatcttaagc ggttcaaagg tctctcttga ttatgatcgg
     2521 gcagatggcg ttagcgatga aggagaggca gtcgatacgc tacctggtaa tgtcatgttt
     2581 gaagtcagtg gttcgggaag cgaattttcg gtaaatacaa agatcaatat taccaatgac
     2641 aacaatcgcg gaatcatcca aatgagaggt tcaggatcga aagtcactgt atcgaatggt
     2701 gggaaaatca ccatcaatac agcaactacg ggcggatttc gcttgcaagg agattctagt
     2761 caaatccatg tatcaagtgg tggacaaatc tcggtcaaaa tggctggaga tgacgatcaa
     2821 cccgggaaca atgggatgcg ttttgtagga aaaggccttt ctttgatcgt agaaggagcc
     2881 ggatcagtta tcgacatcga taagcagagt gggcgttcca gcgcagttcg ttttgagaat
     2941 ggtacgcaaa agctgacagt aaccaatgga ggctcattga gggtaagaaa tacaggtgat
     3001 ggcaaatctt acgtgaacag actcaatcaa ggaaatcaag cgatccaatt ttatgacgct
     3061 tctggttttc tagaaagtcc aggttcagct gattttacag tcactggaga aaaatcaaat
     3121 atcgatatac gcgcagattc cggagcaact gtagatacaa ctggagatat tgatttaaat
     3181 tttacggcag gtgaaaaaac ttatttagtc atggtcggga aaacagggga agataagacc
     3241 ggcatcatca gtggggatgt gttgaatgtg gacttagaat ctcctaccta ttttgatttc
     3301 caaaacaaac ggattggaag cgagaccaat ggcataggcg gctgggtatt ccaaggaaat
     3361 ggagattctt ctttaaagat cgcccattca gaaatcgctt tatggcgaaa aacaggcgca
     3421 acattcgata actttgaaaa agacccggat tatttttctg atatgacaga cttatcgatc
     3481 aatgggaccg acttaggcaa tttcgtatca agccctagtg caggtttaac aaatgaattt
     3541 gctcagacag gtgccggaat gaaaaattat aatcgaatca gtgccaataa tcagctcccg
     3601 atgattgaat ccttaagagt gccaacgaat gcagataaaa aaatctatgg gcatgtcgtt
     3661 gttcccgaag gtgtcgaggc aaagccaaga gatgcttgga caggcgaagt agaagtgact
     3721 ttacgtatta cttacgcaga tacgacgaaa tcatctgtag aaattactgc tctcacgcaa
     3781 ggacaaacag atgaaaatga aggggcagga tataacccat ggggagaagg cgaacaaggc
     3841 ggcttgttcg aagttcaagt acccaatgat gagagattaa aaacggagga tcaagtgcaa
     3901 gttgttgctg ctaaaaaagt aagaggtggc caagaagtga cagaactgga ctctgccaaa
     3961 acgacagtcg atgtgattcc tcctgaacct gctaatttaa gtaccacatt gatcaatgca
     4021 gcttctcgca cgatcacagg tacaggtgaa gcaggagcct tggtttcatt gaaaaaaaat
     4081 caagctgttt tgtcagaaac aactattgat gatacagggg aatttgttct ttctatccca
     4141 gaaaatcaac tttttgcagg agatgtatta gaaattgtat taaacgacca agcaggagaa
     4201 gctactgcta agggtgtgca gaacaagcca agtaccaacg atgaaattgg gaatcacaat
     4261 ccaagtgata cgccagttgt ttatcatgat gccccgcctt ttgcacctgc tacgaagatc
     4321 gtagtagaag gtggtctctc atttcttgcc ccttccaaac tcgattttgg tcagatcaaa
     4381 gcaactggtt tgacacaaga agcatttggc caaatggata tacaggaaaa attatggatt
     4441 tatgatcagc gagaaacgaa aaatccgtgg atcttaacga taaaattatc tcagcctttc
     4501 acaaatgatg caggattttc tatgcaggat gtcctatatt tcaagaaagg agaggagtat
     4561 caattagtga atgaagaaga actggaaatt gtcagaggac aaaattcaac gaatgtgcaa
     4621 gcagattatt cagaatattt aaataacggt cgggcgttta aacttgtctt aaccaaagaa
     4681 aatcagatag ctggtaatta ctcagcagag ataacatgga cattagcaga tgcgccgaca
     4741 gaataaagat caaggaggat gattgatgaa tcgaatatgg aataaaagtc atcgaaacaa
     4801 ctgctcttta cttatgatcc ttctccttgg attcatcctt tggcagacac ctgtccatgc
     4861 ctccgaattc aattttgcgg tagattttga attaccggct aatcagattg ataagaacaa
     4921 aacatatata aatgtaagga tggagcctgg gaaaacacag gaaatcccct acacggtctt
     4981 taaccgaaca gataaatcag taacagttga gacattgatc cactccgcta cgacgaattc
     5041 aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt gatgaaacat taagttatga
     5101 tatgaaaaac ttagcaaaaa cagtgggaga aataactgtt cctcctcaaa gtgcggtcac
     5161 tgagacactt tcactgaaag cgccagaaaa agagctggct ggtgtggtcg caggagggat
     5221 cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt acagttggtg tcgtcaatca
     5281 gttttctcaa gtgaaagcaa tcttgatttg aaatagcgaa aaagagtctt ccccagattt
     5341 gaaattaaaa aaagcttatg cggatcaaag cgatggaaga aatgtgatta aggcgaatct
     5401 tcaaaatcca aatgcttctt atatgttcgg cttgactaca tcggttgaaa tctacaaaaa
     5461 agaaacaaaa gagccttacc tttcaataaa aaaagaaaaa atagatgtag cacccaatac
     5521 aaattttgat ttacttgtcc cattagaagg aaaaaaatta gctccgggac attatcaagc
     5581 ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc cataccgatt ttgaaataac
     5641 tgatgatgca gcggaccaat tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac
     5701 atttgactgg cgatggctga tacttgttgg agtcattctt attggcttat ttgctggatt
     5761 atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt
     5821 ctagctggaa gtctattatt atgtacacaa agtgtttttg ctgagaataa gagctcagcc
     5881 actacagaag gaacgatttc tgtgaccaaa ccagatgctg caaatcctga agacgttgtt
     5941 ccagttcctc ctgttgatcc aaatgatcct gatactcctt ttacacctga cgaaccaaat
     6001 cagggaacat ccggattact gacgattgac caagctccag atcttgattt tggaacaatc
     6061 aagttaggta cagcaaaaac agtatatgct gcattacaaa gcggaacgga cagtactgga
     6121 gcacaaacac aagtacagaa tttcgtacaa gtaacggata aaagcggaaa ttatgccggt
     6181 tggaaattat cagtcaaacg aacagaattt attggtacaa catctgatac gaataaacta
     6241 actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa atagcgcaac taatagcagt
     6301 ggaatgccat ctaccgtttc tggaactggc gctgaaggaa tcgaaatccc agcagatcag
     6361 gcagtagatc tagtaactgc aggagcaaat gaagggattg gcacatggat ttattcatta
     6421 ggagagaatg tggaacaagg gagacaatca gtggaattat ttgttcctgt atccaattat
     6481 gctgtagaca actacgtttc aacattcaca tggtcgttaa cagatgctcc tacagaagag
     6541 gaagcgtaaa atcatatcaa cgatttcagg gagtggagcc gattcaaggc ccactccctg
     6601 aaatctgcta tgcttacgtc tcagctctta ttcatcaact cgaagcagtt ttttggaagt
     6661 catggaaacg ttcctttgtc ttttcaaaga tctttcgacc aacgagtaag gagtcaccgt
     6721 tgaaaaattc taacacataa tgagcagaat caattttctg caattgcgat aagttgatac
     6781 agtaagattt taaaagtaaa aagtaagcat acttttctaa ctctgtcttc aattgtttta
     6841 aagaagtatt cactaattta tcttctgtag ttgttttgac cataacttgg tttttcaaac
     6901 ctttgatact ttcaatatat aaaatttccc gaggatacag gacgagactt ccaccttttt
     6961 gatcaataaa gattttatcg acatttacga tctgctgttc tttccaattg atctgttcta
     7021 gcacttgacg aatttgttca gataaattcc tgtcttttct gatatatcca atcggaagga
     7081 ttttccgatt gatgatctct actgcaaaat tcgtataagc ggtgaaaaaa acaatattac
     7141 tatgtgcatt ttttttacgt atttgttcgg cgacatcaat acctgtgagt gattgcttca
     7201 agtcgatatc gataaagaaa acatcgtgat tatcaatagt gcccaactgg atcaactgat
     7261 tgatttgaga gatagaagtg agctgcacga tattctcgat atgatgattg accaattctc
     7321 tgatgatcca ttcgctgtga aactggttat cttctatgat aaaatacttt ttcatatgca
     7381 ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa caatggagga ataagatggt
     7441 cgcaacagtg acactttatt tgacctttac tttgatttta gatttaggta tctatttatt
     7501 cttttcgaaa caactgctag taaaaaacag ctttggttgt atagctttga tggtggtgac
     7561 acttttactt cctatcccta aagtgagctt tccttaccta gttggcttat tattgatgtt
     7621 tcttttttat tttactacga aaaacctgtt tatcagctgt ttgatcagta atcttttttt
     7681 tgctatcact ggactcagct tttttctggt ttttgatctg atggattatt taggaaagag
     7741 acatcttgga acagcaagtt tttttgcact acttatcgca tttattttct atctattagt
     7801 aaaaagtata gacaaacaga cgagatttct caacttgctt tttacttatc caactgaaca
     7861 ctattttgga ggtatttgta ttttattaag ctggggcgtg atttacttgg tattgggaag
     7921 tgttcacttc cgctcatcta attatctgct tctatctctt ttgtcgatag tcatcagttt
     7981 tttttctttg tttttttcca ttatgttgat cagtttccaa gtaaaagaaa gacaacatca
     8041 ggaatcgctt caactgtatc aaataaatga agaatattac catcatctag aggaattcaa
     8101 acatgattat aaaagtctac tgttttcatt aaaaattact cttcaggaaa ataaaaaaga
     8161 aacgttggaa tttttagaaa tattagaaga tcattctgca gctatttttg aaaaaccgca
     8221 aatacaacag ctaacaaata ttgtatcccc agctgtcagg ggtgtctttt taaaatttct
     8281 agaaaaagca gaatcggaga aaatcccgat ccaagtagtt attcctgtcg aagtgaaaac
     8341 aatcccgatt gatctggttg tttttattcg ctgtttatct atttttattt ctaacgcatt
     8401 cgatcatcgt gtagccgatc agagtccgat tacgatcaat ctgctggaag aaaaagaggg
     8461 atcacgtttt agtatagcga acaaggcaaa tccaacgaat ttgacgctat cggagatggt
     8521 caaaagagga aaaacctcta aaaaaaatgg cgggttaggt ctatacagtg cgaaaaaaat
     8581 gttggatgtt tacgaaaatg cagaactaaa aatcgaattt tcaaaacaga atcactattt
     8641 ttttgtagaa atgtatctgg catctagaaa gagcaaatca gcctactgac ttactgtcaa
     8701 aggataagtt tgaggaaagt ctacacggag atagagacaa caacacagca tattcaaaag
     8761 aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt ttgctatgta gttaaacaaa
     8821 gtagttgaca cttccgctac ttagtattag tacgaaagta tacttcatga tactaagtag
     8881 ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct aactgctttg cttacaccag
     8941 ataaagggaa gagcaatatc gcttatttta ggcgtggaga agcaagcaaa tccctacgat
     9001 cacaaaaaaa atgctgaccc agcagttaag agagttagaa caagatgaaa gtatccatcg
     9061 aaaagcctat aaccaagtcc cgcctaaagt agagtactcg ctcaccgaag aaggaaaatc
     9121 attaagagag attctagtag cgttgtctgt ttgggaagaa gaacgtatcg aaaaacaaaa
     9181 agcctcaagg gaagaagaca cgctactgca tacacatggt ggctacctaa attattgatt
     9241 ttctatgaca tttattttaa ataaaagaaa acaggagttt ttttaaaagc atcatctatt
     9301 ttttatgtaa aaaaatggta tgattaatca agtaagaagt gattggaaga aggcaagcaa
     9361 atgaatagta tggaaaaagt caaccagttt agagatgaac gtaattggcg acaatttcat
     9421 aacgaaaaag atttggctat ctctatttct ttggaagcat ctgaattgtt agaacttttt
     9481 caatggaaaa cgccggaaga agtaaaagaa acacaattag aacgaatcaa agaagaactg
     9541 gcagatgtat tgatctattc atacatgatg gcagacaacc ttcacctgga tctagatgag
     9601 atcattgaag aaaaattgat caaaaataat ttgaaatatc ctgtagaaaa aagcgcaggt
     9661 gtacgaaaaa aatacacaga gttgtagaaa tgtgtcattc ggctgttgga aagagcaaaa
     9721 gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa aaaaaaggaa aagcaaaaag
     9781 atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt tgattttcaa
     9841 tgaacagatc aaggattatt ttgtcagaga gacaggagat aaatatgcta tagcgaatgt
     9901 aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact ttgatgcggt
     9961 agaaccaatg acgacagaag gggtcatgag gtcgcaaatg agaggaacag atctaccagt
    10021 gattgctagt atcgcagtcc cttcagtttc aatcaattta ccggttttca aaggcttgga
    10081 taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa tgggaaaagg
    10141 aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgctgttta caccgctaga
    10201 aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt atacatacaa
    10261 aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg aagtcgaggg
    10321 aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc gactagttgt
    10381 acaaggagaa ctggaaagtg ttacatcgat aaaagatgcg acagatgata tgagatctgc
    10441 tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta cagattaaaa
    10501 acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa gagaaacgca
    10561 aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag tttttttatt
    10621 ttttatatga aaaggcttgc ataaataaaa attcgatggt atgatgattt tgaataagaa
    10681 atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag taggcgaatt
    10741 ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata aaattttgaa
    10801 taacaatgtc gtcatttcga aaaatggatt cggggaagaa gtcgtttgta tgggaaaagg
    10861 actagctttt caaaaaaaga ttggggacga gatttcgcca gaagcggtcc aaaaagaatt
    10921 tgttttaaga gattcttttg ctaaaaacca attccagcag ctgatggctg atattccggc
    10981 agaggagatc gaatgggtca aacaagtagt cgaactggct gaagataaac taaaagttga
    11041 attttcgcct aatatttatc tgacattgac cgatcatctt cattatgcga tcacacgagc
    11101 aaaagaaaat atccaactgc ctaatccatt attatttgaa acaaaaaaat tttatccgaa
    11161 agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa caaaagacgg gtgtggaact
    11221 ttctgaggat gaagccggtt ttattgcctt tcattttgtg aatagccaac aaggaaacga
    11281 agacatgcaa gtgacgatga ccgctaccac aatggtaaga gatatactga gtatcatcag
    11341 taaattcttc ggcatcgtgt tcgatgaaga atctttgaat tatcagcgta tcattactca
    11401 tctgcagttt tttgcccagc gttatctcaa aggggaagga tctgacgagc aagatgaatt
    11461 tctctacgca ttagttcaag gaaagtatcc aaaagcgttt cattgtgtcg aacggatcaa
    11521 tgagtatctt cgacaaacgc aaaaacagga aatggggatc gcagagcaga tttatttaac
    11581 gattcatatt caaagagtag taagtgagaa aaaaagcatc caatcataag gattgttact
    11641 gtatggcagg caaaacctgg attgaaaaaa taggttcacg aatgtttgtg actgtttttt
    11701 tcaatctggg tttttatttt ttaaataaaa aagaaagaag gaaaaaacaa tggataatca
    11761 agcagttggt cgtcgtgtat gggaagcggt aggaggacaa aagaatgtca aaagtttagt
    11821 acattgtgct acgcggttgc gattcagatt aaaagatgaa tctttagcag atacgcaaaa
    11881 gttaaaagaa gacccggacg tgatccaagt cgtacaaagt ggcggtcagt atcaagtagt
    11941 gattggcagt aatgtagcag atgtttatca atcgatcgtg gatgaggaag gtcttgcagt
    12001 ttcggaggat ggaaaagaag agtccaaaaa tattttgaat cgtctgatcg atattatctc
    12061 tagtatattt acaccttttc taggggcgat ggcagccgcc ggggtattga aaggattctt
    12121 atcattggca acggtgatgg gctggctgac agctgatagc ggtacttatc aaatattgtt
    12181 tgctgcagca gatggtgtct ttaccttctt acctgtcatg cttgctttta cagcagctaa
    12241 aaagttcaaa gccaaccagt ttttagcagt agcaattgct atggcacttg tgtatccagc
    12301 tatcacagca gttgctggtg cgggagaagc tatcagtttc tttgggatcc cagtgattct
    12361 ttcaccatct ggttatactt cttctgtcat accaatcatt ctagccgtat gggttcaaag
    12421 taaattagag ccattcgtaa aaaaagtgat tccgcaattt ctgcaaatga ttttagttcc
    12481 gcttgtcgtt ttagtagtga tggttccatt aacatttctt gctttaggac caatcgggac
    12541 ggttgccggt aatgctttag gcggtctctt caactcgatt tatggattta gtccaatcgt
    12601 agcaggtcta atcatgggaa gtttatggca agtattcgtg atgttcggca tgcattgggg
    12661 attcgtaccg atcatgtttt tgaatatcga acaatatggg tttgatgtat tgatgccaat
    12721 gcttcttcca gccatcttgg cacaaggtgg ggctgcttta gctgtagcat tgcgaacaaa
    12781 agatacgaaa cttcgagcat tagggatttc ttctacagtg acttcgttat ttggaatcac
    12841 tgaaccaact gtgtatggtg tgacattacc tttgaagaaa ccattcattg ccgcttgtat
    12901 ttctggaggt attggtggag ctattatcgg attttccgga gtaaaagctt tctccagcag
    12961 tttggtcagc ttattgacga ttccgacttt cattaatacg gtggatggtg tagagtcaaa
    13021 tgtgacagta gcagtaatag ctacaggaat tgcttttgtt cttgcattcg taggtacatt
    13081 gattttagga tttgacgaac aaacacaaga caatcaatta gaaaacaaac atgcgaatgc
    13141 aggaaaaccg attacttcag cacggcatac cttaaaaagt cctttaacgg gtaaagtact
    13201 gccattgagt gaagtaccag accaagtttt ttcttctggc gtgatgggaa aaggtatcgc
    13261 tattgatcca gaagtaggag aactagttgc acctgcagac ggagagatca cgactatttt
    13321 tccaactgga catgctgtag ggatcacaac gactgacgga gcggagattt tgattcatat
    13381 cgggatggat acagtagaat taaatgggaa tggctttgag atcttagtca aacaagggga
    13441 tctcgtcaaa gcaggagatt tattgattcg ttttgacatc gaagcaatta gagcagctgg
    13501 ctatagcgta atcactccag ttgtcattac gaatactgat gcatttgccg atatacttga
    13561 attagaccaa aaagagatta ttgcaaacga agatgttctg gcaattgtaa aataaggagg
    13621 aaatcaggat gcaatcacgt ttttcagaaa aatttttatg gggtggagca actgctgcca
    13681 aatcaattag aaggggctta tgatcgagac ggaaaaggac tatccgttgc agatgctatg
    13741 cctggaggta agcagcgctt tgcgattatc ggcagtgaag aatttgactg gacgattgat
    13801 caagaaaaat atatttaccc gaatcatcga ggaatcgatc attacgatcg tttcaaagaa
    13861 gatcttgcct tgtttgcgga aatgggattc aaatgttatc gtttttcgat tgcctggaca
    13921 cgaatttttc caaatggaga cgaaggtact cctaacgaag cgggattaga attttatgat
    13981 cagctgattg atgagtgctt aaaatatgat attgaacctg tgatcacgat ttctcattac
    14041 gaaatgcctc ttcatttggc aaaagaatac ggtggctgga aaaatcgaaa actgatcgac
    14101 ttctatgaac gatttgctca gacagtatta gagagataca gcagtaaagt caaatattgg
    14161 atgactttca atgaaatcaa ttcggccttt cactttccag ctttaagtca aggattagta
    14221 aaaagcaatg gagcaggaga gtaccaaaat attttccaag catggcataa tcaattcgtt
    14281 gcaagtagca aagccgtaaa aatcggccac gaattgcgtt cggatatcca aatcggctgc
    14341 atgattatct atgcaacaac ttacagcatc gatgccaatc cagtcaatca ggcagcaaca
    14401 atgatccaaa accaagaatt caattttttc tgcacggatg tccaagtacg tggagaatac
    14461 cctgcataca cagcgcgtac ccataaaaaa tatggagtcg atccggaaaa attagagcaa
    14521 acggaaaaag acttccagtt gctaaaagaa tatccagtgg actacatcgg atttagttat
    14581 tatatgtcta cggcaatcaa tgagactgat ccacaagcag caacttcaga agggaacttg
    14641 ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat ggggctggca gatcgatcca
    14701 gaagggctga gaatcgcatt gaatgaatta tacaatcgtt accagaaacc attattcatt
    14761 gttgaaaacg gtcttggagc cattgatcat atggaagagg atggcacgat tattgatgat
    14821 tatcggatcg attatttacg tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt
    14881 gtagatttga tgggctatac gccttgggga tgtatcgatc ttgtcagtgc atcaacagga
    14941 gaaatgagta aacgttacgg ctttatctat gtggatctag acgatgaagg aaatgggaca
    15001 ctgaatcgat caaagaaaaa atcttttgat tggtataaag aagtaattgc ctcaaatggc
    15061 gtaaacttgt aattaaagca gctgaatgaa aaagaacaca cttctcacat gtgatttgtg
    15121 ttcttttttt attttttatg tgctattctt ttagtaatag taaagcaaag ggtgacgtta
    15181 tgaaaaggac acgtgtacta tacttggaag tcgcagataa aattaaagaa gatattttct
    15241 caggaaaata tccagtcggt tcgatgttgc cgacagaaag tgaattagaa gaactgtttc
    15301 aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg
    15361 caaagaaaag tggtcgtggg acgacggttc taagcaatcg tccttacaat cgattatcca
    15421 aagcggcaag ctttacgcaa atcttggaga actcccgata tgaagtccgg aaagaaacgc
    15481 tggatttgca attggtccat ataaagaagg aagatccttt gttttccttt ttcggtgagc
    15541 aagctgtttg ttttaagcgc ttatattatt tgaacaatca gccttatatt tattttgttc
    15601 attatcttcc acaagaaatg atgcaattca gcagagaaga atttggcaaa gaatctttgt
    15661 atcgtttgct gacgaaaaaa ggatatacta ttgactcatt ttctgatgat ttctcggcag
    15721 tatttttaac agaagaagaa caaggaattc tgaaaacaga cgcacagatc ggcatcaaac
    15781 ggatacggaa atcgtctacg caaattgggg aaatgattga atattcggag gcggtctacc
    15841 atacggagct gcatccttac catattgatt atgaaacata aataaaaaaa gaggttgtga
    15901 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt
    15961 cgtttggtta ggcttattac cgcagataca cggctgtttc tggattacca tttattctgg
    16021 ataagggggt gtggcacgat tttcagaagg aatctatgaa ccaatcactg tattgatttt
    16081 agcttcgatg gtaaccccat tcggcttgac gatcgcttat ttcttaggga aaatcattcg
    16141 taaaaatata ctcaatcgtc aagaaatcga tacattgaag accgcttttc ctatggggat
    16201 ctgtcagatc acagagggat gtttccctat cgtattgaac gacttggcac gcaatgtcat
    16261 agcaacggga gtcggtggga cagtaggtgg cggattaagc atgttttggg gcgcagatag
    16321 ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg accagaccat gggcattcat
    16381 tggtgcattg ttagctggtt cttttgtcac aggaataacc attttattat tgaaaaaacc
    16441 agtagatccg aatgcagaaa tcatccaaga agaaaaagag gaagaagaca tatcttggga
    16501 tgatttgaca attagttagg agaggcagaa tggattacaa gaaaaaagta caatagatgt
    16561 accttgagac aggaatggta tgaaagaatc agagttagaa agaatcgaat atacagattt
    16621 tggtttagga gcgatcgaaa ctgtccagag catcggcacc ctccttgaaa tgacgatgaa
    16681 ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg
    16741 aaacaaaaaa actttcctgt actattccaa aaaggaaaga aagttattat acagtaaaaa
    16801 aagaaattat tcttcatgct agagatcagt acacgttcga gcccaatatc aaacactagt
    16861 tccaggcagg cgcaacaggt gcggttatca ctgaattttc ttccagcagt gacgattcaa
    16921 gtgatatttt tacaaatcca gctatttatc gttaacaaaa agatttttag gagaagctga
    16981 tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg cttttttatt tagagaaaca
    17041 tcatttatca gtgaaacata acacgagaac aaaatgattt tttcgttaat aaaagaaaca
    17101 agtatttgtt gtttaaaata atacacgtta ttggcatata ggacgaaatc ccagtatctt
    17161 ctataagtaa gtcatttcat atcgatgaaa acacgttatt tatttttgtt ttatctataa
    17221 aatggctgaa aaat
//
LOCUS       DADOOS010000054        17211 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.054, whole genome shotgun sequence.
ACCESSION   DADOOS010000054 DADOOS010000000
VERSION     DADOOS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.054"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            77..337
                     /locus_tag="J8C37_001497"
     CDS             77..337
                     /locus_tag="J8C37_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304942.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(699..938)
                     /locus_tag="J8C37_001498"
     CDS             complement(699..938)
                     /locus_tag="J8C37_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304943.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1224..1538
                     /locus_tag="J8C37_001499"
     CDS             1224..1538
                     /locus_tag="J8C37_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304944.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1972..2338)
                     /gene="ssrA"
                     /locus_tag="J8C37_001500"
     tmRNA           complement(1972..2338)
                     /gene="ssrA"
                     /locus_tag="J8C37_001500"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2509..3282)
                     /locus_tag="J8C37_001501"
     CDS             complement(2509..3282)
                     /locus_tag="J8C37_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5304945.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3426..3776
                     /locus_tag="J8C37_001502"
     CDS             3426..3776
                     /locus_tag="J8C37_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAZ5304946.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3757..4473
                     /locus_tag="J8C37_001503"
     CDS             3757..4473
                     /locus_tag="J8C37_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5304947.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4473..5921
                     /locus_tag="J8C37_001504"
     CDS             4473..5921
                     /locus_tag="J8C37_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5304948.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5991..6500)
                     /locus_tag="J8C37_001505"
     CDS             complement(5991..6500)
                     /locus_tag="J8C37_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304949.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6490..7215)
                     /locus_tag="J8C37_001506"
     CDS             complement(6490..7215)
                     /locus_tag="J8C37_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304950.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7438..9558)
                     /locus_tag="J8C37_001507"
     CDS             complement(7438..9558)
                     /locus_tag="J8C37_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAZ5304951.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9788..10966
                     /locus_tag="J8C37_001508"
     CDS             9788..10966
                     /locus_tag="J8C37_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAZ5304952.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10982..11740
                     /gene="aroD"
                     /locus_tag="J8C37_001509"
     CDS             10982..11740
                     /gene="aroD"
                     /locus_tag="J8C37_001509"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAZ5304953.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11763..12248
                     /gene="ybaK"
                     /locus_tag="J8C37_001510"
     CDS             11763..12248
                     /gene="ybaK"
                     /locus_tag="J8C37_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAZ5304954.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12319..13572)
                     /locus_tag="J8C37_001511"
     CDS             complement(12319..13572)
                     /locus_tag="J8C37_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ5304955.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13690..15039
                     /gene="gdhA"
                     /locus_tag="J8C37_001512"
     CDS             13690..15039
                     /gene="gdhA"
                     /locus_tag="J8C37_001512"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAZ5304956.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15151..16497
                     /locus_tag="J8C37_001513"
     CDS             15151..16497
                     /locus_tag="J8C37_001513"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAZ5304957.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16805..17191)
                     /locus_tag="J8C37_001514"
     CDS             complement(16805..17191)
                     /locus_tag="J8C37_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ5304958.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5653 a         2999 c         3427 g         5132 t
ORIGIN      
        1 gttattgatt ttcacaatca tattttaatg gtattatcat tttgaataat gaaaaaacat
       61 taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat atatttgcca
      121 acctattggc ttgaaacaaa aagtggttgg agaagtaatc agtaaattag aaaattgtgt
      181 tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg
      241 aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat atctatataa
      301 ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa gtgccttcaa
      361 aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt gttcatgatt
      421 tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc ctggtggaga
      481 aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca aactagtagt
      541 tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat caggatcatt
      601 tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag gagtaaacgc
      661 gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa
      721 taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga aacttttata
      781 tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc gatactattc
      841 gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc agtaagtaac
      901 aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact ccttatagta
      961 tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact atcaagtctt
     1021 ttatagtatc tattagtctt agtaatacat atatggagag ggtgatatcc cagttcaaac
     1081 cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt ttttatgaaa
     1141 aaatttataa gtgaaaacgt aggtatgata ttatctagag gtaatttttt tgggtattta
     1201 aatagttgct aggaggatag cttttggatt tttacagtaa ttttatctta ataatagcaa
     1261 tattattact tctaaatatt tggttttttg ataagagtag aaatgcaggg ataggtttta
     1321 gaaccaaacg aagtacgtct tctgaaaaaa agtgggtata ttcacagact attttttacg
     1381 gaggggttat ttcaatcagt ttactttctt caacacttta ttcttttaat gttatagacg
     1441 tttccatgtc aaattttata tctattattg gaatccttat ttctgcaatt attacacaac
     1501 tacttcttgt gtttgaagag aagtcaaaaa ataattaaag caattatgcc ttttagtaca
     1561 ggaattatta cttcttacaa atgtatagcg aatgtgaagc ctgctgtttt aattgttcta
     1621 gaaaaacttt tgagtgaaca attattttat gttaggatga attaatttct ctagagttta
     1681 taattaaaaa acttatttga aggaacatgc ttaaaccata attgtgtttt tccttccatc
     1741 agcataaaag ttcacctcaa aaagagtgta cgaacaaaag ttctttttgt aaaccttaaa
     1801 atagattttc ctgaatacgt attggggatt tctaaatgtt tgaatgtttt tttgaatgca
     1861 aaatcaatgc aaaaaatttt gtatttctct acttttctct ccttgaccaa aataaaaaaa
     1921 cgttgattta acaacgtttt tcatacatgt ttatctgtct caaccataca tatatggaga
     1981 cggcgggagt cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca
     2041 actcatttaa ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct
     2101 gatgttctct ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac
     2161 ccttactcga gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta
     2221 aagcgtaaga gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga
     2281 cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt
     2341 tagctaacaa gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa
     2401 attcaataaa aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga
     2461 aaaaataagc gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc
     2521 aaaatacttc aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga
     2581 aagttctttt aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa
     2641 caaggcaaaa tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag
     2701 ttctttttgc agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat
     2761 cgagtaagga ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc
     2821 acctgattga gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc
     2881 aatttccgga acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc
     2941 ggtacaccag attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg
     3001 atcatacatt ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc
     3061 tactcgaatg aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat
     3121 agagtggatt tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt
     3181 taatgcgttc atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt
     3241 tccatcaaga tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt
     3301 attggaataa gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc
     3361 ctgaacatat tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg
     3421 aaagaatgat ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga
     3481 ttctgccctc aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa
     3541 cagacgctca ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct
     3601 tactgatggc attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga
     3661 ttgaaatgct tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat
     3721 tacagaaata tgacctggaa actggaggga acgataatga atattttaat gattgaagat
     3781 aatcaatctg tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact
     3841 ttcaagtatg atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat
     3901 atgattacac tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc
     3961 cgcaaagttt caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat
     4021 caggtgatcg gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg
     4081 acgcttattg ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa
     4141 gaagcagcag tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg
     4201 aaaacacgag aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc
     4261 gatctattgt ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt
     4321 gaattagtct gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag
     4381 aaattacgac aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc
     4441 ggctacaaat tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag
     4501 ctttttgcgg aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga
     4561 caaaacaaac gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg
     4621 aaaagacaac gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc
     4681 aaaatgcatt gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta
     4741 tcgataaaaa cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa
     4801 cagctgatca atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa
     4861 atattttagg acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg
     4921 aattctatgg agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc
     4981 caattacact gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca
     5041 gctatgcttt tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag
     5101 aagtgacgaa tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg
     5161 agttagcaga tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg
     5221 aagaacaaga aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac
     5281 ctcttacgac aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc
     5341 agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga
     5401 atgaaaactt ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat
     5461 tcaatggaac agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag
     5521 caggagatac attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc
     5581 gatttgtcca aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc
     5641 aaatcacgat tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg
     5701 gaattggaat gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc
     5761 cttcaagaaa aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac
     5821 ttgtacgctt gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat
     5881 tcgctgttcg tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag
     5941 atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg
     6001 ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag
     6061 ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc
     6121 aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag
     6181 gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta
     6241 agcaaggaag gagagatatc aagtttttct tgataggcca aacgatcttg tttgatttct
     6301 ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa
     6361 tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc
     6421 tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga
     6481 atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa
     6541 agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc
     6601 aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt
     6661 ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg
     6721 cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga
     6781 ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag
     6841 ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggc gccaattgat
     6901 caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa
     6961 tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg
     7021 aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc
     7081 taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct
     7141 tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata
     7201 gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag
     7261 tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag
     7321 aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta
     7381 ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttgtca
     7441 ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc
     7501 aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt
     7561 ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg
     7621 gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac
     7681 agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt
     7741 aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata
     7801 ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa
     7861 tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt
     7921 aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg
     7981 gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga
     8041 acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg
     8101 gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa
     8161 gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc
     8221 atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata
     8281 atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg
     8341 tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt
     8401 aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt
     8461 tcgattccag aatgtaccag cgttcccatg gaataccgca ctagtgtaac cttgtgtttg
     8521 acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga
     8581 gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc
     8641 tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact
     8701 attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat
     8761 caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctt tagcgagccc
     8821 gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat
     8881 atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata
     8941 aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt
     9001 cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc
     9061 taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt
     9121 ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg
     9181 gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat
     9241 cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag
     9301 caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat
     9361 ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa
     9421 gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt
     9481 caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg
     9541 tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcat
     9601 gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt
     9661 caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa
     9721 tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg
     9781 tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc
     9841 actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt
     9901 agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat
     9961 cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt
    10021 tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg
    10081 tatattcaat ggagaaggag acggaatcgg cggtctgatt gttgattatt atgcaggtta
    10141 tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc
    10201 ttttcgtgaa atctacccgg aaatcgtagg aggctatgag aaaattcgat ttgaatcagt
    10261 aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa
    10321 agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt
    10381 agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt
    10441 gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga
    10501 aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt
    10561 gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa
    10621 atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc
    10681 cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag
    10741 tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc
    10801 gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga
    10861 acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg
    10921 caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag
    10981 aatggtttgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct
    11041 tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc
    11101 cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc
    11161 agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg
    11221 taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga
    11281 acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag
    11341 tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag
    11401 tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga
    11461 acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt
    11521 gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta ttattacgat
    11581 gtccatggga aatcttggta aaatttctcg tctggctggc ggaacgacgg gttccgttat
    11641 gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc
    11701 attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt
    11761 ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga
    11821 ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg
    11881 ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg
    11941 tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag
    12001 caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga
    12061 ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt
    12121 tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac
    12181 agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa
    12241 aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc
    12301 ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt
    12361 agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc
    12421 cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag
    12481 agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag
    12541 cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc
    12601 tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat
    12661 accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg
    12721 aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc
    12781 cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat
    12841 accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt
    12901 taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata
    12961 cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac
    13021 tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc
    13081 agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat
    13141 tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag
    13201 ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa
    13261 catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat
    13321 atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg
    13381 tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat
    13441 cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc
    13501 agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata
    13561 cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa
    13621 aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag
    13681 cgtggaacga tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa
    13741 aaggatcaag ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg
    13801 tcttttttgg acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa
    13861 ccagaaagag ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta
    13921 aatagagggt accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc
    13981 ttccatccta gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag
    14041 aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa
    14101 ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa
    14161 catatcggcc catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc
    14221 ggctatttgt tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt
    14281 aaacctttgg atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg
    14341 tattatgtca aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt
    14401 gtttccggaa gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg
    14461 aaagttgtta catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg
    14521 aaactattga aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa
    14581 cgtccttctg ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat
    14641 atcgctttgc catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta
    14701 gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt
    14761 tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga
    14821 gtagcagtat ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag
    14881 caagtggatg agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca
    14941 gcagccgagt acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa
    15001 aaagttgcca aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa
    15061 tattgtcagc attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc
    15121 tgtagagtaa acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa
    15181 gtagcaccgt ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc
    15241 cacaacgaat tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta
    15301 ccaacgaact atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa
    15361 tctgattctg aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca
    15421 gcgatcgact tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca
    15481 ccacaaatct tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa
    15541 gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa
    15601 ccagcgattg cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa
    15661 gcaaacaaac gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca
    15721 gatgcagaag gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta
    15781 ttaactgctg ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg
    15841 caaggtgccg ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct
    15901 taccaatacg cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg
    15961 atcaactatg aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa
    16021 tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg
    16081 cactctttag gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag
    16141 gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta
    16201 ggctacttgc aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg
    16261 cttgctcata cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct
    16321 tatacattgg gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg
    16381 aatggaatca acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca
    16441 cttctaggca aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat
    16501 aagggttttt cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa
    16561 actggtaccc atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg
    16621 acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta
    16681 gtttgtttta ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg
    16741 ttttctagaa tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct
    16801 tagcttaaga taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta
    16861 cttttttctc tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt
    16921 aagcagaacc attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt
    16981 caccgttttc agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat
    17041 tatctgcttt gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt
    17101 tacctacata agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata
    17161 tgataacgat caaccctaaa atttttttca tgaaagatct ccttttttat t
//
LOCUS       DADOOS010000055        17210 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.055, whole genome shotgun sequence.
ACCESSION   DADOOS010000055 DADOOS010000000
VERSION     DADOOS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.055"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(97..1116)
                     /locus_tag="J8C37_001515"
     CDS             complement(97..1116)
                     /locus_tag="J8C37_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5304959.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="J8C37_001516"
     CDS             complement(1138..2145)
                     /locus_tag="J8C37_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAZ5304960.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="J8C37_001517"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="J8C37_001517"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAZ5304961.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="J8C37_001518"
     CDS             complement(2796..5090)
                     /locus_tag="J8C37_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAZ5304962.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="J8C37_001519"
     CDS             5350..7515
                     /locus_tag="J8C37_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5304963.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="J8C37_001520"
     CDS             7819..8652
                     /locus_tag="J8C37_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAZ5304964.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="J8C37_001521"
     CDS             complement(8754..10454)
                     /locus_tag="J8C37_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304965.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="J8C37_001522"
     CDS             10961..12121
                     /locus_tag="J8C37_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ5304966.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="J8C37_001523"
     CDS             12137..12580
                     /locus_tag="J8C37_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5304967.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="J8C37_001524"
     CDS             complement(13063..13593)
                     /locus_tag="J8C37_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAZ5304968.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="J8C37_001525"
     CDS             13698..14249
                     /locus_tag="J8C37_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ5304969.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="J8C37_001526"
     CDS             complement(14402..14875)
                     /locus_tag="J8C37_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ5304970.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="J8C37_001527"
     CDS             15013..16485
                     /locus_tag="J8C37_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ5304971.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="J8C37_001528"
     CDS             16482..17192
                     /locus_tag="J8C37_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAZ5304972.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5353 a         3093 c         3536 g         5228 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc gtacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag gatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgacactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggaaaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataagt actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtcaggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atctcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt tctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctgttttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaacaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacaccaacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg cttgatccaa
    15361 gcttgcggcg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta
//
LOCUS       DADOOS010000056        16961 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.056, whole genome shotgun sequence.
ACCESSION   DADOOS010000056 DADOOS010000000
VERSION     DADOOS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.056"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(112..1314)
                     /locus_tag="J8C37_001529"
     CDS             complement(112..1314)
                     /locus_tag="J8C37_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5304973.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1431..1946
                     /locus_tag="J8C37_001530"
     CDS             1431..1946
                     /locus_tag="J8C37_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ5304974.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2145..2591)
                     /locus_tag="J8C37_001531"
     CDS             complement(2145..2591)
                     /locus_tag="J8C37_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAZ5304975.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2638..4116)
                     /gene="msr"
                     /locus_tag="J8C37_001532"
     CDS             complement(2638..4116)
                     /gene="msr"
                     /locus_tag="J8C37_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAZ5304976.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4470..5099)
                     /locus_tag="J8C37_001533"
     CDS             complement(4470..5099)
                     /locus_tag="J8C37_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ5304977.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(5083..5547)
                     /locus_tag="J8C37_001534"
     CDS             complement(5083..5547)
                     /locus_tag="J8C37_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5304978.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(6034..7302)
                     /locus_tag="J8C37_001535"
     CDS             complement(6034..7302)
                     /locus_tag="J8C37_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAZ5304979.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7533..8270)
                     /locus_tag="J8C37_001536"
     CDS             complement(7533..8270)
                     /locus_tag="J8C37_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5304980.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8337..8906)
                     /locus_tag="J8C37_001537"
     CDS             complement(8337..8906)
                     /locus_tag="J8C37_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAZ5304981.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9034..9906
                     /locus_tag="J8C37_001538"
     CDS             9034..9906
                     /locus_tag="J8C37_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ5304982.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10248..11120
                     /locus_tag="J8C37_001539"
     CDS             10248..11120
                     /locus_tag="J8C37_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5304983.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11117..13075
                     /locus_tag="J8C37_001540"
     CDS             11117..13075
                     /locus_tag="J8C37_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5304984.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13407..14258)
                     /locus_tag="J8C37_001541"
     CDS             complement(13407..14258)
                     /locus_tag="J8C37_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5304985.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14381..14848
                     /locus_tag="J8C37_001542"
     CDS             14381..14848
                     /locus_tag="J8C37_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAZ5304986.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(15111..15593)
                     /locus_tag="J8C37_001543"
     CDS             complement(15111..15593)
                     /locus_tag="J8C37_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ5304987.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15693..16556)
                     /locus_tag="J8C37_001544"
     CDS             complement(15693..16556)
                     /locus_tag="J8C37_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAZ5304988.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         5068 a         3507 c         3017 g         5369 t
ORIGIN      
        1 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
       61 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
      121 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
      181 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
      241 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
      301 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
      361 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
      421 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
      481 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
      541 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
      601 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
      661 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
      721 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
      781 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
      841 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
      901 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
      961 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
     1021 aacgatgata aacaatcctg tcatcaatgt ttttttcaaa ccgatccgac tagataagaa
     1081 acctgtaaat agcatcacca caccagtgac taacatgtaa ctagtaaata acagggtcgt
     1141 ttcagctggc gtagcgttta actgtgcagc aatcgttttt aagattgggt ctactagacc
     1201 cactcccata aaagcgatca tacaggtagc tgctacaact agcgctgaac ttttcccctt
     1261 tgtttcttca tttgtttgtt cttgtatatc tatttgattc tccaatattt ccatcccttt
     1321 ctctctatta gcatcataca tgatttattt tacatgtcaa tatgcacatg taaaacgtgg
     1381 aaactttact gtgatataat aaagaaaata agacgagagg taggattttt atgaatactt
     1441 tagcctatat attattatgc gtgcttttat ccaaaccacg ttctgggtat gaattgaagc
     1501 agctgatcac aattttttgg gaagcccatc acagtcagat ctatacaacg cttgccaagt
     1561 tagaccagca gggttatgtg accgttttgg acaaagacga gcactctcaa aaaaagatct
     1621 accacttgac tgatgaagga cgtgctcttg tagaggaatg gatcaaagag gaaactcccg
     1681 ctcctacgca aaaagatgaa tttctagcaa aaatctatgc tttttcgacg ctcgataaaa
     1741 caacagctac tggactgcta ttcactagag aacaacagct gaacaaaatc atgcaaaaaa
     1801 atcaaaaaaa attgaatgac ctctcgctag acaagaaaca agaatttgga cgttatgttg
     1861 tgatccagcg cagaattatg ctttgtcagc aggaactcca atggtgtcag caagtaagag
     1921 aacaaatgca gcagtttttt gagtaaggga taaacagaac ctttctagaa atatctattg
     1981 aagagcaaat tatcttcaat aaaagagcaa tacagttgga aaagaaaaag cgatccgaac
     2041 gacaggccaa aacctatcgt tcggatcact atttttctag aactgcattc aagagactat
     2101 gacaaaaagc atgccaaggc cttttttcaa ataacagtgt tccatcattc attactttga
     2161 attgctttta gcacacttct tgaaagcggg ccaacgatga tcaactgcaa cggtaaagcg
     2221 gcaatcacat tcattttcca agcattcata taagcagaga ataattgatc aggtattcct
     2281 tgttccatga tcacaccgaa taaagacata catgtcacca ttcctaaaac catcaagcat
     2341 gaaatagtta agatcatttg gaccttgctt tctggattta ctggtagttt aaaggcaatt
     2401 tttttcgcca gcacaccgat gatgaagaca tccaaaatga aagccaccac gaatccaggc
     2461 actagaccaa tcaacaaact tgataaatga agatcttgat gcagccataa attatacgcg
     2521 ctcatcccca agaccatcaa aaaacacatc attgttgtaa aaattattcc ttctttttta
     2581 tttgtaggca atctgtacca ctcctttaaa tttgtcacga aaatcatcgt agcacattta
     2641 aaaatttctc gtaagtactt tttttatttt ctgccctttt atttcataaa cttcatcagc
     2701 cgtacgctcg acaaagtaag aatcatgaga agtaaacaaa atcgttcccg gatatatttg
     2761 cattagcttc tctaaagctt cgatcgttgc catatcaata aaattagtcg gttcatctaa
     2821 cagcaacaca ttacttggct tagtaaaaag caaagcaatc gtcagacggg tcgcttctcc
     2881 cccacttaat gtacataacg gtttcgtcaa agtttcctga gcaaacccta agtgattcag
     2941 caaactacgg acaagcgatt ctgaaaactc tgtttgtctc atgacaaaac gaatgatgga
     3001 ttcttcagat gtcatttgat aacccttttg ctgatatact tgaaagctta ctttcggaga
     3061 gagaaggatt ccttctcctt gtttgcggat atgttctaat aaggttgtct tacctgaacc
     3121 attttcaccg acaatcgcga tttttttacc taaagaaaac tgaaaatcac catttaccag
     3181 taatgttctc ccacttcttt ccaattggac attttgtccc atgatgggat agcggctgtg
     3241 gagttcaaga gattttggta ctggaaaacg gatttgtttc atctgctgcg gcttggttgt
     3301 ttctcctatt ttttggagtc ttctctctaa tgttttcgca ttcttttgga tggctttttg
     3361 tacactgtct ttttgtttag aggaagacaa ccgatccagt ctgatttgtt gctttttctt
     3421 tttactggac actttttcca acttttccgc ttgttttttc ttttcttgga tcgatttttc
     3481 caatcgtttt ttctctttca tgatcgactc tgcttctcgt agctgagtct ttttctccaa
     3541 ttccttttga gtgagatagg cactataatt ccccgaaaac tctcggattt ctccgtcttt
     3601 tacttcccag attttcgatg ctagctcatc tagaaaatag cgatcatgac taacaaaaac
     3661 cagcgtacca taataataac gaagctcttc aatcagtcga tcaatacttt ttctatctag
     3721 atgggttgtt ggctcgtcta gaagcatccc ttgttcataa gaagaaagaa gacgtatcaa
     3781 ctcgatcttt cgttcttccc ctccgctcca tttccgctct gaagtttctt ccagtcttaa
     3841 ctggaaatga ctggctaatt ccaaatttag gtcctttgct tcagcgagat aggttaattg
     3901 aggcaggtag ttgaaggtga tctctgtttg gattttccca tgatcgggaa aaagttcacc
     3961 ggctattagt ttgagcaaag tcgatttacc gacaccattt tctcctatga tccctatacg
     4021 atcttgctga tagacacgaa gagaatcaat agatagttct agctggttgc caaagctgac
     4081 ttgtagattt gttatattta ctgctaaatt ttccatatgt tttacactcc tatttcatgt
     4141 aaaacaacaa ttatcgatac taaaaaagag cagaaaaatc tgtttgcaaa aaaactgcat
     4201 cacatctttt cctactctga acgaaataac tatgactaaa tcaccaaatc tgtcatagct
     4261 ttcacctatt ctactgtcgt tcgaaaaaat ggacatactc catccatgaa attgttgttt
     4321 atacatgtct tttgaattta tttagttgtt attcaaaagt tcgaaacgta atttcatcga
     4381 tgcagtcatt tttccgacct ccttttttat taggttacca cacacgattc aaaaaaagca
     4441 atagctactg aatagtaact gaaaaccttt tattcgaaca tagtgatctt ttctggtgct
     4501 aaacgaacag actcgtaacc ttcttctacc gcttttccta ttgataaaat aataactgga
     4561 acataacggt ctttatccaa accaaatgcc tctgccaact gatctgcttc gaatccacca
     4621 attggatttg tttcatagcc atgatcacgc gcaactaaca tgaattgcat agccgctaag
     4681 ctagaatcga ctttgactac atcgttcatt tcttctcttg aaaaactctt gtaataagga
     4741 atgatcgcag ctaattgttg atctctgact tcttttggca tcttccctga ttcgtaagct
     4801 tggttgtaga tttcttcgcc atattcgtaa cattgcatat cgccaaaaat catgaccatt
     4861 gcagaagaag tatcattttg tctcgtattg aaacggatca aaggacgcaa gatttctttt
     4921 gcttcctcgc tttcagcgac cacaaagcgc catggtttca tattgacaga agaaggtgca
     4981 gtcgtcgctt ctttgatcat ttccagcatt tcttcgtgtg ggattttgta tgtttcatca
     5041 tatacgcgaa cggatcttct accagtcata attgttgaaa aatcattttt ttgttccatt
     5101 attattaacc tctttcgttt ctgttgttat attgcaaagt tgggtattca atttatctaa
     5161 aagtgttaga aattgtttca tttctacatc gctgaaagcc ggggatagca cggaacctgt
     5221 atctttatgg tccatttcac aatgcttgat tttcttctgg gctttttctg ttagcgctac
     5281 atacacttcc cggttgttct gaggatttct ctgacgcgaa acatagccat ttgcttccaa
     5341 taccttcaga tgacgagtaa ctgctccttg atcaatctgc aacttttctt gcaaactgct
     5401 ttgtagcaat ggattttctt ctttcagtct catcatgatc tcgtatctag taagacttat
     5461 accgatattt tcttcaaaga tctggacgat tttttgatcg gctaatttga tttgatataa
     5521 aaggtgacta atctcttcta aagacataat ttcctccttt ctaataattg actcgtcaac
     5581 gattgacttg tcaataatag aataaaaaaa gaaaaaagtc aaagaatcag ctgtgtaata
     5641 aaaaaaacgc tctcaataga aaagtataga gaaaagaaat tttttgtaga atgaacggaa
     5701 aaaatgataa agaaagcaca acgctaaaac caagattatt gattcttatc aaactataga
     5761 agatagctct tctgcagtag ataattttac accgccaaaa atttccgaaa tcgccttttg
     5821 caagaatccc gtcgaatatt agaaattgga tcagtatgct taaacagtaa taaaaaagtt
     5881 atgacaagat gttgtcacaa ccttttttga gtaaacggtg ttcaaaaact tgcatctgcg
     5941 gtaataagtt caaccaaaca aaaaacatga aaatctgttt ttgtttggct gttcttacta
     6001 cttgatgcag ggcagctttt tcactacctc tttttaataa ccataaaatc ctattccttc
     6061 atatctccag cccatcggtg ttagccaatc tctttcctta gcactcaagg tatagtgatg
     6121 ggtaccagtt ttttcatttg gattaaatag tcgataaatt ggcttggaac caccagaata
     6181 aaaacttaga ccttcataac gccaaccgat ttttgttaaa gagttgatct cagattgtgc
     6241 cattgtgtaa tgatggtctc cactgtaagg attgtataaa cggtagacag gttgtccgct
     6301 gtttggtgct tgccaagcaa tcccttcata ttcccagcct acagttggca gataattttt
     6361 ttctccggca ttcaatgtat ataaatgttc tcctgtatga cggttataca gacgatacat
     6421 cggagaagtt gaagttggtg ctgtatcata ctgtgtcaga ttataggttt cgattacatt
     6481 gttcaatgta gaaccataat taggcgcagt ggcataacga cctgttagcc aagccgtagc
     6541 atctttgtaa gagttcgtat tgcttttcca tgcaccggaa tagtagtaaa cgcctggctg
     6601 aaaagaagtc gtcttcaata ccgttacgtt atcttgcaat gattctttgt acgaaggata
     6661 ttttctaaaa gcggcattca ccgtaatcca ttgcccattc acatattccg aagtaggcat
     6721 cgtaacagat tggccattat agcttccttt aatcccaaat agattgtaat taggggcaga
     6781 agccaaagta ctgttccccc agccactttc aacgatggct tgagcaatca tgactgatgc
     6841 atataagtca ttggcacttg ccaaactttg tgcagttgag gcaatactat caatgaacgc
     6901 ttgttgagaa ttagcagatg agcgagcata aaaagcatag ccagtatagt tttcgaattc
     6961 agacaaatct gcttgatggg tatgtgtcaa tgtttgccca tctgtcaatt ggttcgtgat
     7021 ttcttctgca ttctctggat tgttctgagc ttctgctgat gtactgtctt cggtttgtgt
     7081 ttgttctgtt gtttcagttg ttggttcttc ggttgaaatt gtattttctg agacagtagc
     7141 tgactgcaaa gtactttctg tatcgttttc aattcctgtt tctgaagaac tcgatgtctg
     7201 agttgcgaca gtttctgtcg attcttcagc tagcactgtt gtgttggcaa ctgctagatt
     7261 tcctaaaaga ataacgctaa ataataaatt ctttgctttc atcttttttc tcctttgaat
     7321 tatgaaactt ttcctaaata acagtgtaac taaaaaagta aaagcaagtc aatgtccata
     7381 tttaaataac atttgtttct ttttaataaa tttgcttttt ctgcataaaa agacgtaaat
     7441 gaagaatatt cttctccatt cacgccttaa attgatggtc gtttgaataa ctatttaaca
     7501 agtaattctc tctggatatt atccgttatg gattatactt cctgtgtcgt cggacggata
     7561 atcatgttgc tgacagacat ccggtctggc gtttctatcg caaatgctac agcagaagct
     7621 atatcttcag atgttaatcc ccattctttt tgcgcttcat gaagttcaag agcagccttt
     7681 ttgtcgctga tggtttgata taattcagtt tgaacagctc ctggagagat gatcgtagat
     7741 ttgatgttat tttgccgttg ctcttgacgt aatccttcca tgattgcacg gacagcaaat
     7801 tttgttccgc aataaacggc ggaatcagga taaacaacat gtcccgccac agaatcagtt
     7861 gaaatgatat gcccagattt ttgtttttcc atgattggaa gtacggcgga aattccattt
     7921 aacacaccca tgatgttgat atcaagcata ttttgccatt catcacggtg accttcaact
     7981 aatggagcgg ttggcatgat tcctgcatta ttgtacaata catcgattcg accgaacttt
     8041 tccatcgcta aatcgataac tgcttggact tgtgcaaaat ctcttacatc tgctgtttga
     8101 atatatagct cagcttctgg taaagattct tttattgctt ttaggcgatc ttctcggcgt
     8161 gcagctataa ctagtttagc ccctttttct gctaatagct tcgttgttgc ttcaccgatt
     8221 ccgcttgaag cgcccatgat gacaatcact ttttctgata aatcattcat tctattctcc
     8281 tcctgttttg aaaaatctct tgcttatact cactatctct tatttcatct tactgttcaa
     8341 ataatccgtt cgacaggttt gacgattttg gcagggattc ctgcaacgat ggtattgtct
     8401 gggacatctt tcgtaacagt tgcattcgct gcaacaatgg cattctcgcc aatcgttacg
     8461 cctggcagaa tagttgctcc tgctccgatc caagcatttt tcttgatgtg gattggcttt
     8521 actgacaagc ctcttcgttc ttttggatcg gttagatggt tcaccgtgat gaggttgaca
     8581 cgaggaccaa tcaatactga atcttctaaa cagatgccac ctaagtcaac gaacatcact
     8641 tgacgattga taaagacatt tttccctata ctaatatgtt tgccgaaatc tgtataaaaa
     8701 ggcagcagaa cagtcaccgt atcatctact ttgtcactaa tgatattttc tagccgtttg
     8761 cggatatctg ctggttcatg gtacccagta ttcagcgctg ctaaaggctc ttcgttttcc
     8821 gcttggatct gatggatcac ttcaaagagc ggatccgtta ctcgaatctc tttattgacg
     8881 atttgttttt cgatgaggct tgtcattttg ctccctcctt ttatccttac tttataacgg
     8941 acggcttatg atgtctaata cttataaaac atacttaact attcctttta actatactta
     9001 gtggtatgct tttttaaaaa caggagggaa aaaatggata ttcgtatact gacgtatttt
     9061 ttagcagtag ctagagaaaa aaatatcagc cgagcagcaa aatctctgca tatcacgcaa
     9121 cctactctct caaaacaact gaaagattta gaagaagaac ttggtgtgac acttttcact
     9181 cgaggctccc gagagataca gttgacggat tctggtcgat atctttattc aaaaggaaaa
     9241 gaaatcttgt cgttggtaga aaaaacggcg aacaatttaa caaatgacgc aatcatctct
     9301 ggagaaatct acattggcgg aggcgaaact aaagcaatct ctttcatttc tgatagcctt
     9361 cattcgttga tcaaggcgca tcctgatatc cagtttcatt tatatagcgg gaatgcagat
     9421 gatatcatag acaaactgga tagcggatta cttgattttg gactcgtaat cgaaccgaca
     9481 aacaaacaaa aataccagtc atttcgactg ccagcccaag atcgttgggg acttttgata
     9541 catcggaatc atcctttagc aaagcaaaca gtcatccatc cgcaggaact gaaaactgtt
     9601 cctttgatca tttcccggca aacgtcagtg gacagtcaac tagctgaatg gctaggtttc
     9661 agcatagatc agttatcgat cgtcggaaca tataacttat tgtacaatgc ttctttgatg
     9721 gtcgataacg ggacttttgg tgcattagct ctagatggga tcatcaatac ttatgggacg
     9781 gaaaaagtat tcatcccact cgatccgcca ttacaagctt cactgaatct catttggaaa
     9841 aaagaccagc ctctttctcc tgctgctcgt aaatttttag atgtcctaac ggagaaatac
     9901 agttaagtag agtttttttc cttcgctttc agctttttaa caacgaataa gttaagcaaa
     9961 agatccaatg attaaataac ataagtttct taatttgttc cttcctttta tttctgataa
    10021 ttccagtaaa ttactataat tataaaaaaa gaattgacaa aacataactg tctatataaa
    10081 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
    10141 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
    10201 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
    10261 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
    10321 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
    10381 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
    10441 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
    10501 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
    10561 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
    10621 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
    10681 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
    10741 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
    10801 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
    10861 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
    10921 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
    10981 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
    11041 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
    11101 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
    11161 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
    11221 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
    11281 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
    11341 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
    11401 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
    11461 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
    11521 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
    11581 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
    11641 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
    11701 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
    11761 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
    11821 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
    11881 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
    11941 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
    12001 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
    12061 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
    12121 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
    12181 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
    12241 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
    12301 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
    12361 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
    12421 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
    12481 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
    12541 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
    12601 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
    12661 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
    12721 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
    12781 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
    12841 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
    12901 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
    12961 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
    13021 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
    13081 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
    13141 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
    13201 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
    13261 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
    13321 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttcatact caaaactaat
    13381 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    13441 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    13501 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    13561 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
    13621 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    13681 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    13741 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    13801 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    13861 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    13921 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    13981 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    14041 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    14101 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    14161 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    14221 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    14281 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    14341 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    14401 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    14461 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    14521 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    14581 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    14641 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    14701 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    14761 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    14821 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    14881 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    14941 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    15001 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    15061 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    15121 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    15181 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    15241 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
    15301 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
    15361 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    15421 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    15481 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    15541 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    15601 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    15661 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    15721 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    15781 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    15841 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    15901 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    15961 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
    16021 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    16081 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    16141 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    16201 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
    16261 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    16321 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
    16381 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    16441 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    16501 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    16561 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    16621 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    16681 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    16741 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    16801 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    16861 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    16921 tatttctaac aaaatcactt catttttgtc agataaataa a
//
LOCUS       DADOOS010000057        16833 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.057, whole genome shotgun sequence.
ACCESSION   DADOOS010000057 DADOOS010000000
VERSION     DADOOS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.057"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..562
                     /locus_tag="J8C37_001545"
     CDS             <1..562
                     /locus_tag="J8C37_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ5304989.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="J8C37_001546"
     CDS             762..1076
                     /locus_tag="J8C37_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ5304990.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="J8C37_001547"
     CDS             1097..1474
                     /locus_tag="J8C37_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ5304991.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="J8C37_001548"
     CDS             1484..2257
                     /locus_tag="J8C37_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304992.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="J8C37_001549"
     CDS             2279..3682
                     /locus_tag="J8C37_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAZ5304993.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="J8C37_001550"
     CDS             3864..5048
                     /locus_tag="J8C37_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAZ5304994.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="J8C37_001551"
     CDS             5045..5335
                     /locus_tag="J8C37_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304995.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="J8C37_001552"
     CDS             5332..5553
                     /locus_tag="J8C37_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304996.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="J8C37_001553"
     CDS             5595..6374
                     /locus_tag="J8C37_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAZ5304997.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="J8C37_001554"
     CDS             6441..6935
                     /locus_tag="J8C37_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ5304998.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="J8C37_001555"
     CDS             6991..7629
                     /locus_tag="J8C37_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5304999.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="J8C37_001556"
     CDS             7701..8096
                     /locus_tag="J8C37_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305000.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="J8C37_001557"
     CDS             8080..10533
                     /locus_tag="J8C37_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ5305001.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="J8C37_001558"
     CDS             10530..12557
                     /locus_tag="J8C37_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305002.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="J8C37_001559"
     CDS             12554..13576
                     /locus_tag="J8C37_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAZ5305003.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="J8C37_001560"
     CDS             13593..14522
                     /locus_tag="J8C37_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305004.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="J8C37_001561"
     CDS             14767..14883
                     /locus_tag="J8C37_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAZ5305005.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..16818
                     /gene="tet(M)"
                     /locus_tag="J8C37_001562"
     CDS             14899..16818
                     /gene="tet(M)"
                     /locus_tag="J8C37_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAZ5305006.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT         5726 a         2780 c         3336 g         4991 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatata tacgccttta tagtggagta
    15721 ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg
    15781 tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt
    15841 attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca
    15901 cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa
    15961 cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt
    16021 ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta
    16081 caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa
    16141 gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc
    16201 gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg
    16261 ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa
    16321 aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg ttggaatgtg
    16381 acggattgta aaatctgttt taagtacggt ttatactata gccctgttag tactccagca
    16441 gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc tggaacagaa
    16501 ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct ttcacgggca
    16561 tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag
    16621 gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa tgatttaact
    16681 ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca ggttaccact
    16741 ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt aagatatatg
    16801 ttcaataaaa taacttagtg cgttttatgt tgt
//
LOCUS       DADOOS010000058        16819 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.058, whole genome shotgun sequence.
ACCESSION   DADOOS010000058 DADOOS010000000
VERSION     DADOOS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.058"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..455)
                     /locus_tag="J8C37_001563"
     CDS             complement(<1..455)
                     /locus_tag="J8C37_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAZ5305007.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="J8C37_001564"
     CDS             complement(560..1423)
                     /locus_tag="J8C37_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAZ5305008.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="J8C37_001565"
     CDS             complement(1619..3718)
                     /locus_tag="J8C37_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAZ5305009.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="J8C37_001566"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="J8C37_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAZ5305010.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="J8C37_001567"
     CDS             complement(5071..6912)
                     /locus_tag="J8C37_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAZ5305011.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9487)
                     /locus_tag="J8C37_001568"
     CDS             complement(7094..9487)
                     /locus_tag="J8C37_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ5305012.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="J8C37_001569"
     CDS             complement(9594..10454)
                     /locus_tag="J8C37_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAZ5305013.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="J8C37_001570"
     CDS             complement(10513..11034)
                     /locus_tag="J8C37_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAZ5305014.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11354..12073
                     /locus_tag="J8C37_001571"
     CDS             11354..12073
                     /locus_tag="J8C37_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5305015.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="J8C37_001572"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="J8C37_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAZ5305016.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="J8C37_001573"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="J8C37_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAZ5305017.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="J8C37_001574"
     CDS             16433..16789
                     /locus_tag="J8C37_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ5305018.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5766 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaa
//
LOCUS       DADOOS010000059        16804 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.059, whole genome shotgun sequence.
ACCESSION   DADOOS010000059 DADOOS010000000
VERSION     DADOOS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.059"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..245
                     /locus_tag="J8C37_001575"
     CDS             <1..245
                     /locus_tag="J8C37_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305019.1"
                     /translation="VVSIFYFIGNMPETYYSQGYFYTVFILMGIGGLIHSLVDYNIAY
                     FIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(369..1478)
                     /gene="ugpC"
                     /locus_tag="J8C37_001576"
     CDS             complement(369..1478)
                     /gene="ugpC"
                     /locus_tag="J8C37_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAZ5305020.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            1687..2109
                     /gene="mgsA"
                     /locus_tag="J8C37_001577"
     CDS             1687..2109
                     /gene="mgsA"
                     /locus_tag="J8C37_001577"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAZ5305021.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            2432..2968
                     /locus_tag="J8C37_001578"
     CDS             2432..2968
                     /locus_tag="J8C37_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAZ5305022.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(3008..4771)
                     /locus_tag="J8C37_001579"
     CDS             complement(3008..4771)
                     /locus_tag="J8C37_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305023.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(4774..6489)
                     /locus_tag="J8C37_001580"
     CDS             complement(4774..6489)
                     /locus_tag="J8C37_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305024.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(7268..7492)
                     /locus_tag="J8C37_001581"
     CDS             complement(7268..7492)
                     /locus_tag="J8C37_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305025.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            7692..10460
                     /locus_tag="J8C37_001582"
     CDS             7692..10460
                     /locus_tag="J8C37_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5305026.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            10541..10960
                     /locus_tag="J8C37_001583"
     CDS             10541..10960
                     /locus_tag="J8C37_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAZ5305027.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            10972..11442
                     /locus_tag="J8C37_001584"
     CDS             10972..11442
                     /locus_tag="J8C37_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305028.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            11459..12238
                     /locus_tag="J8C37_001585"
     CDS             11459..12238
                     /locus_tag="J8C37_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5305029.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            12225..13046
                     /locus_tag="J8C37_001586"
     CDS             12225..13046
                     /locus_tag="J8C37_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5305030.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            13063..14112
                     /locus_tag="J8C37_001587"
     CDS             13063..14112
                     /locus_tag="J8C37_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ5305031.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            14125..15129
                     /locus_tag="J8C37_001588"
     CDS             14125..15129
                     /locus_tag="J8C37_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ5305032.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            15762..16781
                     /locus_tag="J8C37_001589"
     CDS             15762..16781
                     /locus_tag="J8C37_001589"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAZ5305033.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         5425 a         2955 c         3413 g         5011 t
ORIGIN      
        1 tagtagtcag catcttttac tttattggta atatgcctga aacatattat tctcaaggat
       61 atttttatac agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt
      121 ataatattgc ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta
      181 cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat
      241 attagacatg atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa
      301 gatgagagtt ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag
      361 ggggcaattt atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg
      421 ctgatgttga aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact
      481 tttgagatga actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat
      541 tcggaaacga caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt
      601 tcactgtgga tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt
      661 tcaactaata ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg
      721 ataacaccat ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg
      781 gcaacgaaca tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata
      841 aatccgtctt tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga
      901 gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg
      961 gctacacgta atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca
     1021 tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct
     1081 ggttttcgtt tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt
     1141 ttgatgtctg ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca
     1201 gtcatatgag gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt
     1261 gccacgtcat tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt
     1321 cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata
     1381 aattcacggt ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca
     1441 tattttttat atacgttttt cagagccatt tctaccataa tacgttccac actttctttt
     1501 ttagttttat taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt
     1561 gaacaagaat tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca
     1621 taagaaataa aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg
     1681 tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta
     1741 gcaacagcgt ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg
     1801 cagaggatca cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc
     1861 ggggatcagc aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt
     1921 cgagacccgc tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct
     1981 gatgtctatg agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga
     2041 ctggaagccg gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt
     2101 tcattttgat ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat
     2161 aaaagctaaa caaactgata atatgcgata taatacgaac gtattggata atataaacaa
     2221 taatattagt ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa
     2281 agatcccaaa aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt
     2341 tgcctcttgg acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac
     2401 gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat
     2461 gattgctatt atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat
     2521 cgagacccca tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct
     2581 ttttggtcct ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct
     2641 cttctcgaaa tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat
     2701 ctacggattc tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct
     2761 tagtgtcacg ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca
     2821 tgtgccattg ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt
     2881 accgattcaa tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt
     2941 aagaagttta gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg
     3001 cttgttttta ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt
     3061 gaagcagctc atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct
     3121 ggtccgcatt tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca
     3181 tcattgctaa gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag
     3241 cttcatctaa gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt
     3301 ggcgttgtcc atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg
     3361 gcaaacgttc tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt
     3421 cacttgcttc cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag
     3481 tatcttgtaa caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt
     3541 catggtcatc gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa
     3601 caagtgtgga ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag
     3661 cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat
     3721 caaaagagac ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag
     3781 gcttttcttc tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt
     3841 gcagttgtgt cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga
     3901 tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc
     3961 cgccaactaa accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg
     4021 tcaaggaaga agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt
     4081 caaattgctg ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga
     4141 ttcgttcggt cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg
     4201 tttgtgaagc acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa
     4261 cgaccaatgt cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg
     4321 ccactccaga aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat
     4381 catttgtaaa tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca
     4441 atcgattcag atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc
     4501 gattgcttaa cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga
     4561 atagaataag taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat
     4621 cgatactttg accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta
     4681 gtatcacagc tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga
     4741 gcgtctgcaa ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat
     4801 ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt
     4861 accttgatcc atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat
     4921 aataatcact gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg
     4981 ataatccaac gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc
     5041 tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac
     5101 ttttgtatct aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag
     5161 agcttcccaa cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat
     5221 agtcccggta aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc
     5281 cagctgccaa gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata
     5341 gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc
     5401 aaggactgta tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg
     5461 agtataacgg aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt
     5521 agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt
     5581 tcctgaagct tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa
     5641 aagcatttga ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat
     5701 atttactttg atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt
     5761 tgtagcaggt gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc
     5821 atttgtgctt ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac
     5881 acgaacaccg ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac
     5941 tttttggtag agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg
     6001 caataatgct aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga
     6061 cccgatactt aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg
     6121 aagctgattg atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa
     6181 ctcgttgtga gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa
     6241 tccttgtgaa gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac
     6301 agacatagcg accatccata agcccatttc aatgattttt cctcggtcat tcatttttag
     6361 tccattatcg atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag
     6421 aaatttgaag aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa
     6481 ctgaatcata tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc
     6541 acattgtagc ttaggaaatt tagaatgaaa agagaacaag ccttttcata aatgaaagtt
     6601 ttattatgaa gaagctttag aaattgaata gactattttt tttaaaaaaa agaaaatgag
     6661 aaaacatgcc agtctcttca ttcataggaa cgtctatttt acttaatttt tctataaatg
     6721 aattctgcat aaaaaatata tcaagtaaat gtttatagat gaaactctat ttacttcttt
     6781 tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag
     6841 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
     6901 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
     6961 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
     7021 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
     7081 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
     7141 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
     7201 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
     7261 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
     7321 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
     7381 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
     7441 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
     7501 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
     7561 cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt
     7621 tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag
     7681 gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt
     7741 tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg
     7801 taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca
     7861 aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta
     7921 agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg
     7981 cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta
     8041 tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag
     8101 gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc
     8161 atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata
     8221 atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag
     8281 aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg
     8341 tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga
     8401 tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta
     8461 cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa
     8521 tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt
     8581 tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt
     8641 cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag
     8701 tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc
     8761 atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa
     8821 tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc
     8881 agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc
     8941 tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt
     9001 ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag
     9061 agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg
     9121 ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata
     9181 ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc
     9241 actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta
     9301 tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac
     9361 tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag
     9421 taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac
     9481 cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat
     9541 tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt
     9601 tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg
     9661 aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc
     9721 agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt
     9781 gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta
     9841 cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca
     9901 gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata
     9961 tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac
    10021 tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca
    10081 tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga
    10141 aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca
    10201 tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga
    10261 tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag
    10321 aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga
    10381 tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct
    10441 cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt
    10501 gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt
    10561 agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa
    10621 aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac
    10681 agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt
    10741 tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt
    10801 tgccggattg aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga
    10861 gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa
    10921 cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta
    10981 ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca
    11041 ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag
    11101 acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac
    11161 tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa
    11221 tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta
    11281 ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca
    11341 gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa
    11401 gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat
    11461 gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact
    11521 agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg
    11581 tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt
    11641 ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt
    11701 tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac
    11761 gttgacattg atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa
    11821 agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc
    11881 cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag
    11941 caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt
    12001 agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa
    12061 acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt
    12121 gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt
    12181 ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga
    12241 tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa
    12301 tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc
    12361 cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc
    12421 tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa
    12481 tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa
    12541 catctttgat gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat
    12601 tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct
    12661 tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg
    12721 gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt
    12781 tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga
    12841 tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta
    12901 tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc
    12961 tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt
    13021 ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat
    13081 tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca
    13141 agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac
    13201 gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa
    13261 gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct
    13321 gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa
    13381 gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca
    13441 caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct
    13501 gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc
    13561 agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca
    13621 gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca
    13681 cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg
    13741 aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac
    13801 ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta
    13861 aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc
    13921 accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat
    13981 gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca
    14041 gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa
    14101 agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca
    14161 ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg
    14221 gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa
    14281 gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa
    14341 tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt
    14401 accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca
    14461 ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc
    14521 acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg
    14581 tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga acgttattta
    14641 ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa
    14701 aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt
    14761 tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat
    14821 gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta
    14881 tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa
    14941 atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa
    15001 aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat
    15061 gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt
    15121 gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc
    15181 tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta
    15241 tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta
    15301 tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt
    15361 gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc
    15421 aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc
    15481 tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt
    15541 aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag
    15601 tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata
    15661 aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata
    15721 ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag
    15781 ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg
    15841 atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa
    15901 atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg
    15961 cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt
    16021 cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta
    16081 aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag
    16141 aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag
    16201 tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac
    16261 aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag
    16321 aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag
    16381 gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc
    16441 ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact
    16501 tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac
    16561 agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa
    16621 cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa
    16681 caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt
    16741 tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa
    16801 tcgt
//
LOCUS       DADOOS010000060        16381 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.060, whole genome shotgun sequence.
ACCESSION   DADOOS010000060 DADOOS010000000
VERSION     DADOOS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.060"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..541
                     /gene="hslV"
                     /locus_tag="J8C37_001590"
     CDS             <1..541
                     /gene="hslV"
                     /locus_tag="J8C37_001590"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAZ5305034.1"
                     /translation="SPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVRRIY
                     NGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEAMLI
                     VMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAENAL
                     NIAADICIFTNHNIIVEEL"
     gene            555..1955
                     /gene="hslU"
                     /locus_tag="J8C37_001591"
     CDS             555..1955
                     /gene="hslU"
                     /locus_tag="J8C37_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAZ5305035.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1973..2764
                     /gene="codY"
                     /locus_tag="J8C37_001592"
     CDS             1973..2764
                     /gene="codY"
                     /locus_tag="J8C37_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAZ5305036.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2846..3721
                     /locus_tag="J8C37_001593"
     CDS             2846..3721
                     /locus_tag="J8C37_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAZ5305037.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3853..4491)
                     /gene="plsY"
                     /locus_tag="J8C37_001594"
     CDS             complement(3853..4491)
                     /gene="plsY"
                     /locus_tag="J8C37_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAZ5305038.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4618..5052
                     /locus_tag="J8C37_001595"
     CDS             4618..5052
                     /locus_tag="J8C37_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAZ5305039.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5218..7263
                     /gene="parE"
                     /locus_tag="J8C37_001596"
     CDS             5218..7263
                     /gene="parE"
                     /locus_tag="J8C37_001596"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAZ5305040.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7275..9725
                     /gene="parC"
                     /locus_tag="J8C37_001597"
     CDS             7275..9725
                     /gene="parC"
                     /locus_tag="J8C37_001597"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAZ5305041.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10002..12233
                     /gene="pflB"
                     /locus_tag="J8C37_001598"
     CDS             10002..12233
                     /gene="pflB"
                     /locus_tag="J8C37_001598"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAZ5305042.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12477..13238
                     /gene="pflA"
                     /locus_tag="J8C37_001599"
     CDS             12477..13238
                     /gene="pflA"
                     /locus_tag="J8C37_001599"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAZ5305043.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13323..14249
                     /locus_tag="J8C37_001600"
     CDS             13323..14249
                     /locus_tag="J8C37_001600"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAZ5305044.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14431..15324
                     /locus_tag="J8C37_001601"
     CDS             14431..15324
                     /locus_tag="J8C37_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAZ5305045.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15458..15583
                     /locus_tag="J8C37_001602"
     CDS             15458..15583
                     /locus_tag="J8C37_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305046.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15717..16349)
                     /locus_tag="J8C37_001603"
     CDS             complement(15717..16349)
                     /locus_tag="J8C37_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305047.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5821 a         2789 c         3491 g         4280 t
ORIGIN      
        1 atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat ttgctatggc
       61 tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg caaagaaagt
      121 acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg cagatgcgtt
      181 tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc tacaacgcgc
      241 agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac ttgaagcgat
      301 gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag aagttatcac
      361 accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg ctgcagcccg
      421 agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag aaaatgcgtt
      481 aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag aagaactgta
      541 agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta agtgaattag
      601 atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct ttgcgtaacc
      661 gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca aaaaatatgc
      721 tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg gcaaaaatcg
      781 tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt tatgtcggaa
      841 gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc gtagaaaaag
      901 agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt ttggtaaaaa
      961 tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt gaacaaatga
     1021 tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa gtgacagaag
     1081 atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta ctggatagtc
     1141 gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg aacaatggat
     1201 tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg aagaaaaaag
     1261 tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa gaatcagcaa
     1321 aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa gaaagtggaa
     1381 ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct ggagaagtat
     1441 ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa gtaaacacaa
     1501 aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct ttccatttgt
     1561 ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg gtagaattag
     1621 atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat gctttagtaa
     1681 aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca aaagaagcaa
     1741 ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat atcggcgcac
     1801 gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa gcaccagata
     1861 tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt ggctcaattg
     1921 cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga caatgacaga
     1981 cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata cttttgacat
     2041 gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc ttgacagcaa
     2101 cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc acgatgtgaa
     2161 caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt atactgatac
     2221 agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata ttactgcatt
     2281 tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc cgatctttgg
     2341 agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt ttgcagatga
     2401 cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc tttatcagaa
     2461 gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg caatcaacac
     2521 attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg atggagatga
     2581 aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt ctgtgatcgt
     2641 caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt taggcatgaa
     2701 gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa aacacagtta
     2761 ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc attttaaaaa
     2821 gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc agctgatcgc
     2881 cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac atggtttaga
     2941 gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt tattcccttt
     3001 tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc aaatggggca
     3061 acacggattt gctcgggata tggattttga agtcattgaa catggacaag aaatggctag
     3121 tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt ttgaattagt
     3181 gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg caaataccgg
     3241 tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac ctcttgaaga
     3301 aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa gattacgcct
     3361 accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga caaatacgaa
     3421 tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa caaggggtct
     3481 aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa gctataaaga
     3541 tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg tttgtatcga
     3601 accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg aaaaagtagg
     3661 gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca cagtgaagta
     3721 agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt aaaaaagcta
     3781 tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg
     3841 aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc
     3901 agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac
     3961 taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata
     4021 aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac
     4081 catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg
     4141 attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc
     4201 aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc
     4261 agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag
     4321 caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt
     4381 accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga
     4441 aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc
     4501 tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa
     4561 aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg
     4621 tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt
     4681 gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg
     4741 caagacaatg gatataaaat cataccagta aatccattat tggctggtga agaaattcta
     4801 ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta
     4861 tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa
     4921 gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca
     4981 ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa
     5041 aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag
     5101 cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc
     5161 tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg
     5221 gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag
     5281 gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat
     5341 ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag
     5401 attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg
     5461 cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat
     5521 gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga
     5581 gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta
     5641 gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga
     5701 aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt
     5761 acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta
     5821 aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt
     5881 cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg
     5941 acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa
     6001 tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat
     6061 gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg
     6121 agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa
     6181 gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa
     6241 acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa
     6301 caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca
     6361 atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg
     6421 aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga
     6481 aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa
     6541 caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc
     6601 gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg
     6661 attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc
     6721 atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct tactttcttt
     6781 tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta
     6841 tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac
     6901 gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga
     6961 cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg
     7021 ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg
     7081 ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa
     7141 gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat
     7201 gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa
     7261 taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga
     7321 tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata
     7381 tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca
     7441 atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt
     7501 atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga
     7561 agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac
     7621 ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag
     7681 atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa
     7741 cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg
     7801 gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata
     7861 tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact
     7921 ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac
     7981 gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc
     8041 aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg
     8101 atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata
     8161 gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga
     8221 attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg
     8281 accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc
     8341 gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc
     8401 atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc
     8461 gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag
     8521 aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca
     8581 cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc
     8641 tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa
     8701 attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata
     8761 cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga
     8821 agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag
     8881 gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa
     8941 ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg
     9001 ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg
     9061 ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat
     9121 tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc
     9181 tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag
     9241 atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt
     9301 taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag
     9361 atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa
     9421 tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac
     9481 gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct
     9541 atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa
     9601 cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg
     9661 atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt
     9721 cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc
     9781 tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca
     9841 atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt
     9901 gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt
     9961 aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat
    10021 ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca
    10081 ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat
    10141 gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg
    10201 acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg
    10261 aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg
    10321 gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga
    10381 cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg
    10441 ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc
    10501 gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg
    10561 aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc
    10621 gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg
    10681 cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc
    10741 tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac
    10801 gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag
    10861 aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg
    10921 cgcgtacacc tgaatataac caattattct caggattccc tatctgggct actttatcca
    10981 ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc
    11041 atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac
    11101 accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc
    11161 aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt
    11221 gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact
    11281 tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag
    11341 ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata
    11401 acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc
    11461 attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact
    11521 tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag
    11581 cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg
    11641 aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt
    11701 catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa
    11761 aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa
    11821 atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca
    11881 atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct
    11941 gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac
    12001 aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt
    12061 atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa
    12121 tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga
    12181 caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc
    12241 gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc
    12301 gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg
    12361 tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt
    12421 cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg
    12481 aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg
    12541 gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc
    12601 cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag
    12661 cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac
    12721 ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc
    12781 atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt
    12841 ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg
    12901 aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt
    12961 tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt
    13021 atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag
    13081 tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat
    13141 atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc
    13201 ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag
    13261 tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg
    13321 acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag
    13381 ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag
    13441 gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag
    13501 tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc
    13561 aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta
    13621 ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa
    13681 gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag
    13741 gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc
    13801 ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt
    13861 atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc
    13921 ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca
    13981 atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag
    14041 atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca
    14101 gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa
    14161 caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg
    14221 tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa
    14281 tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg
    14341 agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag ccttttaagt
    14401 aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa taaggaaaac
    14461 ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta tctatatgaa
    14521 catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt
    14581 tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat
    14641 catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct
    14701 gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga
    14761 gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta
    14821 agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt
    14881 atcacacttt cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag
    14941 aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta
    15001 aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa
    15061 tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag
    15121 gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa
    15181 ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc
    15241 ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga
    15301 acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag
    15361 gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc
    15421 acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg
    15481 tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga
    15541 ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga agtccatttg
    15601 ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt
    15661 tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag
    15721 gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg
    15781 acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt
    15841 ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact
    15901 aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta
    15961 aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta
    16021 aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa
    16081 gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac
    16141 agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata
    16201 tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg
    16261 tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc taaatgtaaa
    16321 aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga taagtatata
    16381 a
//
LOCUS       DADOOS010000061        16271 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.061, whole genome shotgun sequence.
ACCESSION   DADOOS010000061 DADOOS010000000
VERSION     DADOOS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.061"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(56..712)
                     /locus_tag="J8C37_001604"
     CDS             complement(56..712)
                     /locus_tag="J8C37_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAZ5305048.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            905..1183
                     /locus_tag="J8C37_001605"
     CDS             905..1183
                     /locus_tag="J8C37_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305049.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="J8C37_001606"
     CDS             1202..2410
                     /locus_tag="J8C37_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5305050.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="J8C37_001607"
     CDS             complement(2558..2923)
                     /locus_tag="J8C37_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAZ5305051.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="J8C37_001608"
     CDS             complement(3070..3843)
                     /locus_tag="J8C37_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ5305052.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="J8C37_001609"
     CDS             complement(4017..4877)
                     /locus_tag="J8C37_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAZ5305053.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="J8C37_001610"
     CDS             complement(4877..6295)
                     /locus_tag="J8C37_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5305054.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="J8C37_001611"
     CDS             complement(6352..6801)
                     /locus_tag="J8C37_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5305055.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="J8C37_001612"
     CDS             complement(7246..7509)
                     /locus_tag="J8C37_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAZ5305056.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /locus_tag="J8C37_001613"
     CDS             complement(7608..8903)
                     /locus_tag="J8C37_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAZ5305057.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="J8C37_001614"
     CDS             complement(8900..10171)
                     /locus_tag="J8C37_001614"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAZ5305058.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="J8C37_001615"
     CDS             complement(10336..11205)
                     /locus_tag="J8C37_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAZ5305059.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11497..13107)
                     /locus_tag="J8C37_001616"
     CDS             complement(11497..13107)
                     /locus_tag="J8C37_001616"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAZ5305060.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="J8C37_001617"
     CDS             complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="J8C37_001617"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAZ5305061.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15668..16060)
                     /locus_tag="J8C37_001618"
     CDS             complement(15668..16060)
                     /locus_tag="J8C37_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ5305062.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4639 a         3449 c         2766 g         5417 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    11341 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    11401 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    11461 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    11521 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    11581 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    11641 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    11701 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    11761 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    11821 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    11881 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    11941 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    12001 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    12061 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    12121 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    12181 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    12241 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    12301 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    12361 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    12421 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    12481 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    12541 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    12601 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    12661 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    12721 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    12781 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    12841 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    12901 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    12961 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    13021 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    13081 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    13141 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    13201 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    13261 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    13321 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    13381 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    13441 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    13501 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    13561 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    13621 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    13681 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    13741 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    13801 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    13861 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    13921 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    13981 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    14041 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    14101 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    14161 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    14221 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    14281 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    14341 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    14401 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    14461 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    14521 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    14581 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    14641 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    14701 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    14761 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    14821 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    14881 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    14941 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
    15001 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    15061 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    15121 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    15181 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    15241 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    15301 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    15361 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    15421 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    15481 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    15541 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    15601 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    15661 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    15721 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    15781 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    15841 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    15901 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    15961 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    16021 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    16081 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    16141 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    16201 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
    16261 gtgtttttat t
//
LOCUS       DADOOS010000062        15938 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.062, whole genome shotgun sequence.
ACCESSION   DADOOS010000062 DADOOS010000000
VERSION     DADOOS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.062"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(377..799)
                     /locus_tag="J8C37_001619"
     CDS             complement(377..799)
                     /locus_tag="J8C37_001619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAZ5305063.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(813..2219)
                     /gene="atpD"
                     /locus_tag="J8C37_001620"
     CDS             complement(813..2219)
                     /gene="atpD"
                     /locus_tag="J8C37_001620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAZ5305064.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2242..3144)
                     /locus_tag="J8C37_001621"
     CDS             complement(2242..3144)
                     /locus_tag="J8C37_001621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAZ5305065.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3158..4714)
                     /locus_tag="J8C37_001622"
     CDS             complement(3158..4714)
                     /locus_tag="J8C37_001622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAZ5305066.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4737..5279)
                     /locus_tag="J8C37_001623"
     CDS             complement(4737..5279)
                     /locus_tag="J8C37_001623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAZ5305067.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5266..5790)
                     /gene="atpF"
                     /locus_tag="J8C37_001624"
     CDS             complement(5266..5790)
                     /gene="atpF"
                     /locus_tag="J8C37_001624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAZ5305068.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5881..6096)
                     /gene="atpE"
                     /locus_tag="J8C37_001625"
     CDS             complement(5881..6096)
                     /gene="atpE"
                     /locus_tag="J8C37_001625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAZ5305069.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6149..6868)
                     /gene="atpB"
                     /locus_tag="J8C37_001626"
     CDS             complement(6149..6868)
                     /gene="atpB"
                     /locus_tag="J8C37_001626"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAZ5305070.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7170..10013)
                     /gene="rnr"
                     /locus_tag="J8C37_001627"
                     /pseudo
     CDS             complement(7170..10013)
                     /gene="rnr"
                     /locus_tag="J8C37_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10016..10762)
                     /locus_tag="J8C37_001628"
     CDS             complement(10016..10762)
                     /locus_tag="J8C37_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ5305071.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10874..11110)
                     /gene="secG"
                     /locus_tag="J8C37_001629"
     CDS             complement(10874..11110)
                     /gene="secG"
                     /locus_tag="J8C37_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAZ5305072.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11228..11506)
                     /locus_tag="J8C37_001630"
     CDS             complement(11228..11506)
                     /locus_tag="J8C37_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAZ5305073.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11619..12587)
                     /locus_tag="J8C37_001631"
     CDS             complement(11619..12587)
                     /locus_tag="J8C37_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5305074.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12802..12846
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12870..13844
                     /locus_tag="J8C37_001632"
     CDS             12870..13844
                     /locus_tag="J8C37_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAZ5305075.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13849..14424
                     /locus_tag="J8C37_001633"
     CDS             13849..14424
                     /locus_tag="J8C37_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAZ5305076.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14491..15630)
                     /locus_tag="J8C37_001634"
     CDS             complement(14491..15630)
                     /locus_tag="J8C37_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAZ5305077.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4353 a         3440 c         2853 g         5292 t
ORIGIN      
        1 atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt attgacaaag
       61 agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat catctgaccc
      121 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg tttctctaat
      181 actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga agtagcttct
      241 tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      301 atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa acacggataa
      361 acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt gctcggtgca
      421 acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct tcttcgattt
      481 ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca cgttcacgtt
      541 ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt ccgccattaa
      601 cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact tcatcaatcg
      661 tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca gctgtcgtac
      721 gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt accacattta
      781 ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt cgctttttct
      841 acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc atcgtatttg
      901 ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata tgaacctgga
      961 agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg gactctgcgg
     1021 gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa gatagcaatg
     1081 atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc tactttataa
     1141 tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa tggatctacc
     1201 gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc atccaaatgg
     1261 gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata gattgcttgg
     1321 atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg acccatttct
     1381 gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa ggctgatact
     1441 tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac atcttgacct
     1501 tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg catacgtgca
     1561 cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac tcctgaatct
     1621 ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc ggtaaataca
     1681 gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag tacggtttta
     1741 ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg ggcaagcaaa
     1801 tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc gtcaaaggct
     1861 ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt ttctaaatcg
     1921 atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac aggaacagag
     1981 attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt tgattccatc
     2041 gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac aacttttgat
     2101 ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa ggattggtct
     2161 aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc tgaactcatc
     2221 ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa tttcggtaat
     2281 ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct catcaatgat
     2341 attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat gttctgctgt
     2401 tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca ataactgaga
     2461 caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag ttgcttcttc
     2521 tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag ttagcgaatt
     2581 gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt acatagttgt
     2641 tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata gattgcgcag
     2701 ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc cgccaattgc
     2761 gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca tcgtttgttt
     2821 caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga cgatataacc
     2881 cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc ctctaggatt
     2941 ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt ctcttacttt
     3001 tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact tagaagcaga
     3061 caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga tacgtgtctt
     3121 gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga atggatttca
     3181 atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct ttatattcct
     3241 gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg attgtttcaa
     3301 agatatctga atgattggta tccagatatt cgaataattc atgttcgaag tctaaaatac
     3361 tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc actacttgtt
     3421 tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta cggcgtccac
     3481 gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta aatgcttcta
     3541 attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc attgctttga
     3601 tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct ggacgagtac
     3661 ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa ataacgtttg
     3721 ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag gcagtcattg
     3781 aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt gagtgtaagt
     3841 agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc aaggaaagtt
     3901 cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca tgtttcccgt
     3961 tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa agcaacggcg
     4021 ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca tattttttca
     4081 atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca tagatacaga
     4141 tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt ttccctgttt
     4201 tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga gcgtcgattg
     4261 cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg cctggtgcca
     4321 ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca attggctgac
     4381 ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc atgatctttc
     4441 ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg ataatgatac
     4501 ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa aattcaagca
     4561 actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca cctacataag
     4621 ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt tgttctttaa
     4681 tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc tttgtactat
     4741 ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc gattactcga
     4801 tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg ttttaattcg
     4861 actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg ttgttcgtct
     4921 gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc atcatatcga
     4981 cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc ataaacaact
     5041 tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat gatttctcgt
     5101 ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg tacttgttca
     5161 tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt tgaatcgatc
     5221 gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc tagtttcatt
     5281 tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt ctggagataa
     5341 ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat cgtcttttac
     5401 agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac tttttagacg
     5461 agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc cactttcttt
     5521 cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt gttcgcgttc
     5581 ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat cattggcgat
     5641 cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat gcttcaagag
     5701 agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca tcgcattgcc
     5761 aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt gaagtacttc
     5821 tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat actgattcaa
     5881 ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa cagcttcgac
     5941 tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag gttggcgagc
     6001 cattgattcg attgttttag agataacttg accgttacca taaccggctc cgattgctgc
     6061 tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc taaattagat
     6121 aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta tggctcatat
     6181 aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac aaggagaatg
     6241 cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct aagcttatta
     6301 ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca taaagacgta
     6361 aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac ataaaactat
     6421 atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc accccaaata
     6481 agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg tctgctgtcg
     6541 ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa ccgatgttgt
     6601 ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg ctcaattctt
     6661 ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat tctatcgcat
     6721 tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct aagaagaaaa
     6781 caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa attggtccaa
     6841 tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg aaattcacat
     6901 aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac acggtatcat
     6961 accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt ttttatttca
     7021 gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga aaaaacctat
     7081 gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta aaagcaaaag
     7141 caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa tgttcgatca
     7201 atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga ttttttccct
     7261 ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt taaaggaaca
     7321 acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt tttgtgtaat
     7381 aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc ataaggactg
     7441 atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc atcttttaga
     7501 ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat ccctgcttcc
     7561 atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat taattttccc
     7621 tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt cttcttttta
     7681 acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt actatcgcta
     7741 tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt tccttcgcgt
     7801 tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc ttctgcggaa
     7861 acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt tactcgcact
     7921 ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa atgattttca
     7981 acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc tactgtattc
     8041 ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc gaattcttcc
     8101 cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg ttctgtttct
     8161 actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc cgtagcccac
     8221 gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg atggacgatt
     8281 agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc cgctaagcca
     8341 taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag caacatagta
     8401 ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg taaatctttt
     8461 ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc aaaatcaaag
     8521 aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa cggcagattt
     8581 aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga ttcgatcaag
     8641 cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc agcatcaaca
     8701 aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg catcgtttca
     8761 aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc atagcgttgc
     8821 atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt catccgttct
     8881 gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt tggatcaatc
     8941 tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca gataccattc
     9001 gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact tgtccctctt
     9061 tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac atctgcaatg
     9121 tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc atcatctaga
     9181 tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc tctacgtcca
     9241 gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa tacatcatca
     9301 ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat gccagggtca
     9361 ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata ccctttttca
     9421 gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac tccctctgct
     9481 gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac gccgtaaaga
     9541 tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa ctgagtagtc
     9601 gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc tgtatttgct
     9661 cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt cgcttctttt
     9721 gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc tcgttcattt
     9781 gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt tcctttttta
     9841 ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag taatttaaaa
     9901 tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa actgcgtttg
     9961 cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt catatttatt
    10021 cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat tcgcgtcttg
    10081 ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc actcgctgac
    10141 ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca tctttcccag
    10201 cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg attgctgcct
    10261 gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact gctttttcat
    10321 tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat ttttcaaagt
    10381 tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg ccgccaatca
    10441 ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat cctaatacgg
    10501 caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg tctgcccacc
    10561 agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt cctttaaaaa
    10621 gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt cgaacatcgt
    10681 ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt tttgcataaa
    10741 ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac catagtttct
    10801 ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc cgttttgcga
    10861 gaggcttatt caactacttc gaagataaat atgctaggac aaataataag atcatccaga
    10921 tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga gctttttgtt
    10981 tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt tgcttgcttg
    11041 gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt cccaaaatta
    11101 aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt tatcatagat
    11161 aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa actttcagct
    11221 gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca aactcttttt
    11281 ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat tctactccag
    11341 tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca aatgtatggt
    11401 caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc tcttgttgtt
    11461 catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc cgcactcctc
    11521 aaataactga tattttttta ttcctacctt attttagcag tctcctgatc tataaacaat
    11581 tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt tattctttgt
    11641 tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga tgcttccaag
    11701 tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt ttgaatcgtg
    11761 tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa taaaggcaga
    11821 tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc ggcgtatttc
    11881 tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt gattttactt
    11941 aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat aaatcctttg
    12001 accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc cattgatccg
    12061 ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat ccaggcaatt
    12121 aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc tccgtgcgca
    12181 cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc tatatgagac
    12241 atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt attctgctta
    12301 tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt gattgttttg
    12361 ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg ttttttttct
    12421 actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata ccagtcatat
    12481 cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag aaaatggtaa
    12541 ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt acacccgccg
    12601 ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg attctttatt
    12661 ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag ttctcctcaa
    12721 tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac taatttcgtg
    12781 cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat aaaaaactaa
    12841 gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat tgcgatccag
    12901 ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc gaagtagttg
    12961 ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat gtgctgaaat
    13021 gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa aaacctttga
    13081 agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg gaaacaaatt
    13141 tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt ttggctgatt
    13201 atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc aatatcgctt
    13261 ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc tcgatgggtg
    13321 gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac tggtgtgatc
    13381 ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg gttgggttag
    13441 atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt caaatgaatc
    13501 ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc cactggaaac
    13561 aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat tttatcgagg
    13621 gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga ataagtcgtg
    13681 gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt aaaattatga
    13741 caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta aatgcacaag
    13801 aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat gaataaaaac
    13861 aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa ttatattgga
    13921 ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg aacgattttt
    13981 gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag cggtatttta
    14041 agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat tctcacaggc
    14101 ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag cttgaaactg
    14161 ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat cattaccgtt
    14221 cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct attacatggg
    14281 ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga ctaccttgat
    14341 cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca acgcttgata
    14401 tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata atgctaatct
    14461 ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa taattccttg
    14521 atatttttca atcatatcca aataattatc gatttcaaca tactcatcta cttgatgagg
    14581 caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag ctttcgtaaa
    14641 ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc tgaattgttg
    14701 ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt tgttgtaatc
    14761 gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga ttgattgcag
    14821 caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc cttggagctg
    14881 cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa cattatggat
    14941 tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt cagcatttgc
    15001 tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg gcatagagct
    15061 atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt gcgtatacat
    15121 caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta catagcctgc
    15181 tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg ctaataattt
    15241 gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa tcaccattgc
    15301 tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc catggatttc
    15361 tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga catccatatg
    15421 cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa tcaaattatc
    15481 tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca aaagcttatt
    15541 caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga tgatctcttg
    15601 taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat ttctttttat
    15661 tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat gaagaaagag
    15721 agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac gagaggactt
    15781 gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat gctagttatt
    15841 gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa acatcatata
    15901 cagaagctag ttgcaaaaaa aactctctac ctgctgat
//
LOCUS       DADOOS010000063        15709 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.063, whole genome shotgun sequence.
ACCESSION   DADOOS010000063 DADOOS010000000
VERSION     DADOOS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.063"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..1475
                     /locus_tag="J8C37_001635"
     CDS             138..1475
                     /locus_tag="J8C37_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAZ5305078.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="J8C37_001636"
     CDS             1523..3652
                     /locus_tag="J8C37_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAZ5305079.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="J8C37_001637"
     CDS             3627..4316
                     /locus_tag="J8C37_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305080.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="J8C37_001638"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="J8C37_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="J8C37_001639"
     CDS             complement(5546..5740)
                     /locus_tag="J8C37_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305081.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="J8C37_001640"
     CDS             complement(5790..6230)
                     /locus_tag="J8C37_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAZ5305082.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="J8C37_001641"
     CDS             complement(6234..7079)
                     /locus_tag="J8C37_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAZ5305083.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="J8C37_001642"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="J8C37_001642"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAZ5305084.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="J8C37_001643"
     CDS             complement(8703..9650)
                     /locus_tag="J8C37_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAZ5305085.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="J8C37_001644"
     CDS             complement(9873..10223)
                     /locus_tag="J8C37_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAZ5305086.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="J8C37_001645"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="J8C37_001645"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAZ5305087.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="J8C37_001646"
     CDS             11646..11966
                     /locus_tag="J8C37_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ5305088.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="J8C37_001647"
     CDS             11988..12452
                     /locus_tag="J8C37_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ5305089.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="J8C37_001648"
     CDS             12657..13262
                     /locus_tag="J8C37_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAZ5305090.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="J8C37_001649"
     CDS             complement(13301..14590)
                     /locus_tag="J8C37_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAZ5305091.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="J8C37_001650"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="J8C37_001650"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAZ5305092.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4930 a         2914 c         3217 g         4648 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatg
//
LOCUS       DADOOS010000064        15419 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.064, whole genome shotgun sequence.
ACCESSION   DADOOS010000064 DADOOS010000000
VERSION     DADOOS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.064"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            44..1679
                     /locus_tag="J8C37_001651"
                     /pseudo
     CDS             44..1679
                     /locus_tag="J8C37_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1933..2247
                     /locus_tag="J8C37_001652"
                     /pseudo
     CDS             1933..2247
                     /locus_tag="J8C37_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(2552..3112)
                     /locus_tag="J8C37_001653"
     CDS             complement(2552..3112)
                     /locus_tag="J8C37_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5305093.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            3254..3868
                     /locus_tag="J8C37_001654"
     CDS             3254..3868
                     /locus_tag="J8C37_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305094.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4057..5937
                     /locus_tag="J8C37_001655"
     CDS             4057..5937
                     /locus_tag="J8C37_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAZ5305095.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            6016..6243
                     /locus_tag="J8C37_001656"
     CDS             6016..6243
                     /locus_tag="J8C37_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAZ5305096.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            6248..6790
                     /locus_tag="J8C37_001657"
     CDS             6248..6790
                     /locus_tag="J8C37_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAZ5305097.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            7017..7733
                     /locus_tag="J8C37_001658"
     CDS             7017..7733
                     /locus_tag="J8C37_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAZ5305098.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7793..9811)
                     /locus_tag="J8C37_001659"
     CDS             complement(7793..9811)
                     /locus_tag="J8C37_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAZ5305099.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(9824..10822)
                     /locus_tag="J8C37_001660"
     CDS             complement(9824..10822)
                     /locus_tag="J8C37_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305100.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIV
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            11020..12261
                     /gene="sstT"
                     /locus_tag="J8C37_001661"
     CDS             11020..12261
                     /gene="sstT"
                     /locus_tag="J8C37_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAZ5305101.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            12408..13043
                     /locus_tag="J8C37_001662"
     CDS             12408..13043
                     /locus_tag="J8C37_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAZ5305102.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            13234..13974
                     /gene="truA"
                     /locus_tag="J8C37_001663"
     CDS             13234..13974
                     /gene="truA"
                     /locus_tag="J8C37_001663"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAZ5305103.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(14119..>15419)
                     /locus_tag="J8C37_001664"
     CDS             complement(14119..>15419)
                     /locus_tag="J8C37_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305104.1"
                     /translation="MKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLP
                     LILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIA
                     RLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYN
                     NYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQ
                     FVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKD
                     SLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLT
                     IELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQ
                     YTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         5139 a         2437 c         2977 g         4866 t
ORIGIN      
        1 atactagata aagcaatcca tgaaaggagt atataattaa gtaatgcaag taaagaaaaa
       61 aataatattg tccattctcc tagcaattat tgctggactt tattcaatca attattttag
      121 aaatgttcat actatttcta catcggggga tattgccttc cattttgcta gagtcaaagg
      181 gttatcttca attttttctg gtccaatcaa ttttacgacg tttaatcatt atgggagcgg
      241 agtgaattat ttttatccct acttgacctt ttttccggca gtcatttttt attggataag
      301 taataattta atcgttagtt atatcttata tgtttggcta ttgaatgttt gtactatcat
      361 gctgatgttt cattatggac taaaattttt aaaaagaatc gatgcggctt ttatatttag
      421 ctgtttatat acattttatg ggtaccgtac gattgatatt tatcatcgtt ctgctattgc
      481 tgaagcgatt gcacttacgg ttataccgat agttatgtat tatgcttacg cactgattta
      541 tgagaaaaag ccatgtgcaa ttccattagc agtcagtatg agcttactca tttatacaca
      601 tgttctttcc acttttatga gtgtaatagc cgttggctta ctagtcttag gcggctttgt
      661 agtaagtgaa acaagaaaaa aagaaagcat ccggttgatt gtggagttgt caaaggcagt
      721 aggaatgacg attcttttaa cagcatactt ttggtatccg atgttccgac aaatcatggt
      781 tcaatcgatt aatgagccag ataaaacgac cttacaacac caagcattaa atgtctcgga
      841 tagtttgatt ggtgcgatga ataatgacct gacaaccttt acggtaggaa tcattggtat
      901 tatttctttg atctgtccac tcttcttttt taaaggattt aatcgcaaag aaaaagtgat
      961 ttaccttagt gcggtagtct cttggctctt atccaccaaa ttttttccat ggtctttatt
     1021 acaaaataca cccattcaaa tgattcaatt tccttggcga atcttaggct ttcaagtact
     1081 gtttggctcg ttgattttat cgttcgtttt catgaagagc acatcagcta agcaaagaaa
     1141 agtagttaat ataacggcaa ttgttttatt actgttggct gtatctgctg caacaaaagc
     1201 aaactatgaa cataaagttg tcactcagag agatcacttg gtaatgaatc aagaaaatct
     1261 tattcgttac acgacactcc ttcccggagg actttatgac tatgcaccca cggaagcatt
     1321 acaatacaaa gaacatttgc agaatcatga agtaagaatt ggcaaggaat ggcatactat
     1381 gccttatcaa gtgatggatt cgaaaatagt ctatacagta catgaatcta aaggacaaaa
     1441 aataacgctt cctgtatttg attattgggg aacagtcgca aaagtaaatg gacaaaaaag
     1501 agagattgat aataggacta gtgtcgattg aaggaacaca aggagaaaca atcattgaag
     1561 tttcttccat ttatcctcca actatgaagt tagctttttt aattagttta tgtagttacc
     1621 tctttctttt aattagtttt cttatcagga agttacaaca tataaaaata atgatctgat
     1681 tacatttttt tcaatattag cttactagag aataagaaaa agtaagtaat aaaccaaatt
     1741 taaactttat attataataa tgaagcttaa tcttctttat agagaattgg gctattttta
     1801 atggcaaaga ggggaagaag atgtttcaaa acaaaggaca acgctatgtt gtaaaaggag
     1861 taaaggaatc ttactggaat caaaaatctt tattgagaaa attatctttg aatttaagca
     1921 caaagaaatg aaggtacact agatagcaat gaagatatct ttgtcattaa acgtcgctat
     1981 tttacacaag taagtgatga tttgattcat gtcttttcca atgagtggtt tgtttctgag
     2041 aaaaaattac atgcttctaa tctgcaatat atgccaggtg aagatccaat tcctaatatg
     2101 aaagcaatta tcaacagtaa ggattacgaa ggatataaag ccaaacatcc agaagcgaaa
     2161 ccatttaaat acccacaaga aatgaagcga gcatggcgta aaatgttaga tgatgagctg
     2221 attccccttg aaaatgaatt gaggtaaacg agaagaattc aataaactgg atgataaaaa
     2281 tataatatga ctataaattg gttacagctt caacctataa gttgtaataa attagcctaa
     2341 aagtaattta ttaatgatga ggtgaatttg attggtacaa caagatatat cacttagaga
     2401 gggagaagca gcactacaaa ctacttttca ataagtagga attatagaaa ttttactcaa
     2461 aaaatcttca gtaaacaatt tggctaaata agattcagtt gttatacgag aaggttacgg
     2521 ttcttcttaa ctaagcttag tatgttttta ttcaaggcat gattttctct atgtgatcat
     2581 tcagtttctg taagagttct aataattctt ttttggagta cttgtaattg ctttctaccc
     2641 accgattgag aacggataga caaccttgga cgtggtacgt agtaaagaat ctcatttctt
     2701 ctgtgataat cgttttcttg tcttcatata gccatatgtt cagatagttt tcttcaataa
     2761 cttgtttaac tttcttctga aacgaaatat caccatggtt gaggataaat aagcgatata
     2821 aaaaagtatt tgcgtaaaca tagtcaatca agcgagtgta tagttcctca tagctatggg
     2881 ctggatcata agagagaatt tcatttagat cccgaaaaaa gttgctttct aattgatcat
     2941 atagatcata gatatcctta taatgtgtat aaaaagtcga acgatgtaca tctgccgttt
     3001 ctgttagttc gcgtatagtg attgcttgaa tctttttatt tactaataat gtggatagtg
     3061 tttcagccaa agctttttct gtttttttga cgcgtctatc tgtctttttc atttacctat
     3121 ctccttatcc gacatatcaa agcaaagctg tcgcttaatc gacaaactta tagatgattg
     3181 attatagata gtagacactg tgtctattat aatgtaagta taacacaatc attgaagttg
     3241 aggaggcgaa atgatgaaac taacttttaa acccttagaa aagaaagatt acaaaaaagc
     3301 tatctatttt gctgttgaag gaatgaactt tgagagatat atggaaaatg aacttctgct
     3361 gaatctttac ggaagatatt ttttgtatat ggagttgaat cgagcaactc aaattcttgc
     3421 tttgtatgca gaggacgagt tggcaggtgt tttgctggcg gagataaaag gagaaaaaaa
     3481 gaaacatcgt tcttttggac agcaaatata tgttaagata ttcgattttt tacaaaatat
     3541 atttgctaaa gaaagtaaag gagcttacga gacagcaaat gaacaaatgc ttgcagcata
     3601 tttaaagaag cattcgccag atggagagat cattttttta gctgcaaatc ctgatttgaa
     3661 aacaaaaggc atcggtacca agttgctaac agaatttgaa aaaagagaga aaggaaaaga
     3721 aatattcctt tttacagata gtggctgtac gtatcaattc tatgaacgac gtggatttga
     3781 aagactaaag gaaaaaatta gcaagataaa aatcttagat aaagaaatag atctccgatg
     3841 tatgctgtac aggaaaatca ttcaatgaaa atttcgatag aaacgacaat agttggcaag
     3901 atcactttta gtgagtcttc ttgctaacta ttttttgtag tgtattctac caaaagaaaa
     3961 ccgccttttt gactaaactt gatagtggtc aattttttat gatggataag tatctatact
     4021 tagtctcgta agaatgaaag gaaaagaggg agaaagatga aattacaaaa gtggatccaa
     4081 gagcatcgaa atcatatcac cgccttatca gctgtgttga ttatttttgg ttttatcgga
     4141 aaatttactg gttttgtctt gttctataac attgctctag ccatagcttc tattattgcg
     4201 gtcataccta ttatgatcca tgcgtatcag gcagtgaaag tcaaagttat ctcaattgat
     4261 ttactcgtta ctattgctgt gcttggtgcc tttatgatcg gagaatacaa ggagtcagct
     4321 atcgttacat tcttgttttt gtttggtagc tatctggaac aaaaaacatt gaccaaaacg
     4381 cgggagtcaa tcaaggcttt gaccgagatg gccccatcta ctgctagtgt tgtccaagaa
     4441 gatggaacga ctgaagaagt ggaaatcgat gacgtggaag aaggcgatat cgtcttagta
     4501 aaaactggtg gagctattcc tgtggacgga cgagtcattg acgggaaagg gtatgcggat
     4561 gaagccgcag tgactggtga atcacgacaa gtcgtcaaaa tcagtggaga tgaagtcttc
     4621 tctggaacga ttttagcaga tggctacgtg acgattgaag caactaaggt cggagatgat
     4681 acgacctttg ccaaaattat cgaattagta gaagaagcac aagataccaa atcccatgcg
     4741 gaaaaattca ttgatcgatt ttctcagtat tatactccaa ccgttttact tattgcgttg
     4801 gttgttggtc tgattagtcg tgattttcgt ttagcaatca ctgttttggt tcttggttgt
     4861 cctggcgctt tagtcattgg tgcgcctgtt tcgaatgtag caggaatcgg taatggagcg
     4921 aaacggggca tcttagtcaa aggcggtgaa gcaatggata ctttttccaa aatcgatact
     4981 tttgtctttg ataaaactgg tacattgacc aaaggaacta ctgctgtatc cactatcaag
     5041 aactatacag aggatgaagg ggacagctta gctttagtgg ctcgattaga aacgttatcc
     5101 gatcatcctc tggggcgcgc cgtgatccat tatgctcagg agaaagacat tgattttcaa
     5161 caattagcag tcaaagataa tcagacaatc aagggacaag gaatcacagc tgagattgat
     5221 ggtcatacag tttgtgcggg caacaccaaa ttgattgccg ctcacaaact ttctctcaca
     5281 ccaaagcaag tacaagattt acatcaatta caacaaacag gcagctcggt cattattgta
     5341 gcaatcgaca aaaaaatcac tcaaatcata ggtgtgtcag atgtgattcg accagaagtc
     5401 gctgaacaat tagctaagtt gaaacaagca ggagcaaaac agttagtgat gctgacaggt
     5461 gataatcaga tgactgctga ttatgtagct gatatgttag gcattgatga agtccatgct
     5521 gaattattac cagacgaaaa ggttcaattt gtgaaaaaat accaagaaca aggattgcgc
     5581 gttgctttta ttggagatgg aatcaatgat tctccctctt tggctgcggc tgatatcggg
     5641 attgctatgg gatcaggaac ggatgttgct atcgaaacga gtgacgttgt cttgatgcaa
     5701 tctaattttg aatcgttggt tcatgcctac cgcttggcta agaaaactgt tttaaatacc
     5761 cgtgaaaatg tagtgattgc cattggtgtc gtcttatttt tactcaacgg cttatttgcc
     5821 gggtttattt acatggctag tgggatgttc gttcacgaag ctagtatctt gatcgttatc
     5881 tttaacgcga tgcgtttatt gtattttgga cacgaaagca aagaagaaaa atcgtaaaac
     5941 ttgataagaa tcaatttttt tatggaagct gtaatctaga ataagactat caagaaaaag
     6001 gaaggaagaa ctacgatgaa aaaaattatt atgcaattag atacactagc ttgtccaagc
     6061 tgtatgcaaa aaatcgaaca tgcggtttct caacagcctg gggtaagcaa tacgaaagtt
     6121 ttgtttaatg cgagcaaaat caaagcaaac tttgatccag atgaagtcca ccctgaacaa
     6181 ttagctgcag ttgtggaaaa attaggttat gaagtccaat cagcaaaagt aaaagaggcg
     6241 taaatcaatg aatatagtgg aagaaaaatt tgttcaagaa acaaaacaag cagatatcga
     6301 ccatcataaa ccaactgctg gcgccatggt ggggcatgta ttagcaaatt ttaaattaga
     6361 agaaatgaaa ttgatccaag cacaattgta tgttttgggt acatcttcgc aagaattaag
     6421 tacccttttt tcagaatatg ctgatcaaga agcgagatgg ttcaataaaa tctctcatga
     6481 gttagtgatt gaaaatgaaa tcgttccgtc tactttagaa gaagtcatgc gttatggaaa
     6541 aatttacgaa catggtcaaa acaaatattt accagcctca acaatgttgg aaaactttgc
     6601 aaaagatttt gatttccaaa atgtctttat cacacgggca atcaaattag ctgaaaaaga
     6661 agaaaaatat ggcttgcaac aattgcttgt tgaactttta agtttcaata aagaaatcat
     6721 ctaccaagta caaagactcc aaggaaaaac tgtccgacaa gatttggatg aggaagacga
     6781 tgatgactaa aagtctatgt ttcttcaaat acaaatataa agcgtgaggt aaattggaaa
     6841 atcacgaaga ttaaaataaa agaagcaccc gctgatgtat tacagacaac atcagcgggt
     6901 gcttctcagt ttttagaaga tttgaaagat tgatgagaaa atacgaaaaa tcaataaaat
     6961 cacattgctt atttgataga ataaagaagt gggagtaatc gaaaggagtt ttttaattga
     7021 aaaaaaggat tttagtaaca attggtctag cttttttgtt agctggttgt accaacggga
     7081 atgaatcgaa aaaagaatca acaaaagaaa cagcgaaagc aacagttgca actactacaa
     7141 caactagcac agagagcaag tcaactgcag caagtacaga aacagcagat acctctactt
     7201 cgtcagagtc ttccaaaacg actgaaacaa ctcaaacatc tacttcaagt tcttctgtag
     7261 aggcttcatc ttcctcttca gaaagcagcg aacaagttga acaaacactg tggaatgcag
     7321 acaaatccgc acaattagag gcatttatga ccaaatgggg ccaaacaatg ggacaacaat
     7381 acaaaagtta tacgaatcaa atgagtgtgg acttatatgg tcttaaagtc cctcaagcta
     7441 ttttgaacgg ggagtggaag atggctattg gtggagtgcc tgtttctgca gaatggtcag
     7501 aaagtgggac aggtcaagca gactatcaaa tcacagccgt ctattccgat gcagagacag
     7561 aaccatatct gaaaaagcac gtctatctat tcgggtttca gcaaaatcag ccaaaagtat
     7621 tggtgacaca acaaaatcag gggaatcccg ataattatct ttacttcaac gagacagcga
     7681 ataatgaatt gaaaaatggc tttaatcaaa tcgtttatgg acaagcaaat taatgagata
     7741 aacaaggggt tgtgacatgt cttttatcac aaccccttgc tcagtttatt tgttagtctt
     7801 ttattttttg tgtcgcccat gttgcactgt actggttacc tgcaacaaga tcatattgta
     7861 tcagacgata atcttctaga atttcttttt gtttctcagt taaatttttt tcttggataa
     7921 tttgactatc ctctgtaaca aagacctgtt ttccattaaa acgattagac aagctttctg
     7981 taggatcaat tgttaatgca ggtagatgat tagatacttc tgtcaacaac gcatagtaag
     8041 gagttacttt gatattcgca tgctcaagag ccatagcacc aaagttatta ggagcaacaa
     8101 cttctttatt taacgtagct gcttgttcac gactatactt gttgttataa atgaaataat
     8161 ctgtctggtg ctgttctaat ccatattttt tcgaatcatt cccactatag attgcgggta
     8221 aatggtcacc ataaaaaaca aaggtaatcg gtttatcgat tttgtcgatt tcttggatga
     8281 atttttttac tgctgtatcg gtatagtgaa ggccttgcat ataagtttgt aattcttttc
     8341 ttctattagg aatgacagca gttccttcag cagtgtagtt caattcacta tagtaatcat
     8401 cataaggcat atggttttgc atagtggata attggatgaa ttgtgacgtt tccgtattgc
     8461 tttgtaaagc tttcaaggtt tctttgtatg ctgaatcatc cgatatgcgt gggctttgtt
     8521 ctattttatc cgtataagtt aatttatccg gactgtctaa ataatagaac ctgtcaaaac
     8581 caaatttatc aaacacttgt tttctgcgat aaagagcagc tgtaaaagga tggatggcta
     8641 ttttttcatc gaatagatcg gtaatatttg gacttatttt ctgacgctcc actagctggg
     8701 tataaggtgt aactaacgaa gcagacaggt tactgatact caagcctgtc agtccttccc
     8761 attccatgtt agctgttccg ccaccataac cgacggataa catcaatcct cctgttcctt
     8821 ctgttataat gcttttgatg taaggaatag ggtctgtagg tactgtcaga ttagggacac
     8881 gtgcaggatc actgaagctt tcacttaaat tgagaacgat cgtctgattg tttaaccagt
     8941 cacttcttgt ttggttgatt tcatctgcct ttgtgttgta ctttttcatt attccattaa
     9001 ttttttcttt cgaatagcca gcaggttctt ccatgatttt aatgtcgata ttgtttagga
     9061 attgaatgac tggtccgtta tcttttacgg cgccttgctg gttaaagaaa gtcttgttta
     9121 ctttaaataa attaaacagc aagaaagaag gcgaattttt gtgattaatg aaaaagacac
     9181 ccgaaaaaca tcctaataat acagcgatac tgataaatcg acgtttccaa tgaatcttca
     9241 aggaatactg tttttgtagt ctacgttgta caataatact agtaatcaat aataggagaa
     9301 ggatcaatcc gcccacgatt aataagactg ggctaaccat cgataatagt tctgacaatc
     9361 cggtcagcat ttttaaatct gaaggaaaga caggctcttc ccgcatttcc attttgatta
     9421 ctgtcgaaat agtgagcagt aagttaatga tcaaggtagc agtcaaggcg taccaaaaac
     9481 gattggaaat caacaatagt aagaagaaaa atcccataat aatgatgatt gtcaggatca
     9541 tgggtgcttg tcgctgagta aatattttta aggcaacctg gatagtttct ttccaacctt
     9601 cttttggttc taaaacgaaa atctgcacga aggtaaagca gtaaaaaaat gctgcaacta
     9661 aaaaataact tttatgaaca aaaagccaat gaattctttt tttccaacgc tgagttaatt
     9721 gttcaatcga atcaaaagcg aagtattcga tgaaacgatg aatgaaacgg attttttgta
     9781 tataaaataa aaagataact aggagggaca ctgctaataa atatcaaata aaaaactgcc
     9841 cgatatttat cagccatgcc ttccctgagt ttggaaattc acgtttcata aatgtagata
     9901 cgcgataaga aactaaaggg aaattacata taacttgagt gacaatcaaa aatacagcaa
     9961 gaaaatttcc tcttcctttt atacctaatt tatcagatag catttctaaa aataaaaacg
    10021 aagagaaagt ataatacatt gcgaagagag aatatggttt agaaaacctc agcgctgtat
    10081 ctgtatttcc tctgatgatg agtgaaattt gtgtcatcaa aataatcaaa ccgaataaaa
    10141 caatcaagct gataaatact tgaggaagct tatagcggac cttttctttc cagttccatt
    10201 ctttcaaacc ataacccatt aaaaataaat aaaaaatcca taaaatactc tgtccattct
    10261 gtaatgcaaa cgggtcttta ttaaatagag tagagcagcc aactaatcca attgttaaaa
    10321 ataatagtag atatttaatt gttgttttcg ataatttatt taaaaatcca actgtataag
    10381 gaatactaca aaaaagcaaa gtacaagaaa cagcataagt aaagtaattc tgactgatag
    10441 gtgttaggat aagccaataa tcacgaatat tcaagctgcc agcttgtacg taaaaaaaga
    10501 tattgcaaac tagtgtgact attaggaata taaaggcaaa acgacttaaa taatgtaaaa
    10561 cttttaaacg ggcgattttt tgatattggt aacccatgta aagtgggatc aaatttgcac
    10621 caataacaac ggaagtttgt aaaaatgtat agatgtagaa tcgaccaggg tttgttgcat
    10681 atgtaggaat agtgactgct gaaatcaata tacgctgtaa aaccatcatt atacaagcga
    10741 caaagattgt caggaagcca taactctcct tctttatttt tttcaagaat aaacacctca
    10801 caaaaaaatt ggacaaaaac ataagaaaac gtttcctgat aagttgccca ttaataacat
    10861 cctaacttag aatatctcaa aaaacaacgt gtagacagaa aaagtacaaa attagcaaga
    10921 aatggttgtt tgtattgtta gaatttttag aaaattatac ctttagtttc tttgcataaa
    10981 atgatacaat gattgttaaa attgtgaaag ggtggtcatt tgttagagaa gtttttggat
    11041 atgtcgttag ttcaacgtat tgttgttggt attttagtcg gaatattgtt tggtatcttt
    11101 ttccccacat ggacatttat ttcaatattg ggaacgttgt ttgtcagttg cttgaaggca
    11161 atcgcaccta ttctggtttt tttcttgacg atggcgtcaa ttgccaagca taaaattggg
    11221 aataagacat tcgtgaagcc tattttgatc ttatatttag tgggaacatt gctttctgca
    11281 cttgtagcag ttattgtaag ttcaattttt actgttccta ttactttaca agaaacagta
    11341 tctgaaaaag caccgcaaag tttggaaagt gttttatcta ctatgttgac gaatgtcaca
    11401 caaaatcctg ttcaatctct tattgatgcg aattaccttg gcgtcttatt ctgggctgta
    11461 ttgttgggac tagcttttcg agcacgttca gaatcgacaa aagagttggt ggatcagata
    11521 tctatagcgt tgtcacaagt cgtacaagtg attattgcat ttgctccgat agggatttta
    11581 ggattggttt atcaatcaat tgctacgact ggaatcgctg gattggcaga gtatctgcag
    11641 cttttacttg ttttgatcgg tacgatgctg tttgtcgcat tagttgtgta cccattcctc
    11701 acgtttttgt ttattaaaga aaatccctat ccattgattt tcttttgttt gaaagaaagc
    11761 gcacttcccg ctttctttac ccgaagttct gccgcaaata tcccgataaa catgatgttg
    11821 gctgaaagat tgaaattgac aaaagagtct tattccatat cgatcccgtt aggtgctacg
    11881 atcaatatgg gcggtgccgc gattaccatt tccttgatga cgttgactgc tgtacattct
    11941 ttagggattg aagcaccttt tgttttgaaa gtccttttat gtattttgtc tgctttagct
    12001 gcttgtggag cttcaggtgt tgcgggcggt tcactgttat tgattccatt agcatgtagt
    12061 ttgtttggta tctctaatga tgtcgcgatg caaatcgtcg gtattggatt tattatcggt
    12121 gttatccagg attcaatgga aactgcctta aattcgtcaa gtgatctgtt gtttactgca
    12181 ataggagagc taggcgcgcg taaaagaagt ggggaaaaaa tcaaattgaa agattgttta
    12241 gatggattaa gtgaacgttg aacgttagat atttagtatt tttaaaatga ttcgagtttt
    12301 taatgagtta tatagatgtt cgtgtaaata gaggtttctg ttaaatcaag ataaagtcgt
    12361 ttataataac aattaaaagc ataggtaatt gttattggag gcgtatcatg gattttttac
    12421 gtaaatttgg ttttttcttt ttattattag gcgtgctgag tgattttttg actccgtacg
    12481 tattaggatt gtattatcct gaattagatc aaatgaagag cgtgatcagt gtatttggag
    12541 aggtttccag tcctgtaaga aaagcgtttc ttatttggtc tgtggtttca gggatctttt
    12601 tcatcctgtc attgcctgca ttgtatgctg catttgttag tacatctcgt gtgttagcta
    12661 ccctagttac agcagccatc gcgttctatg gtataggtga ttgtatcttt acaggtttgt
    12721 tcagtatcaa tacccatgaa tcttcgtgga atgtctcgac atgggtccat aatatcggat
    12781 caggtttagg ttacagtggt tttttattgt ttccagttct tcttttcttg atctatcgaa
    12841 aaaatgggca aatggacgta agccacttat accttggact gtctgtgatc agtttgcttt
    12901 ttgcggcgat ttatggactg gcaagaattc cttctataaa ttcttggcca ttactgaata
    12961 aaataggttt ttgccaaaga atcagttttt tctttaatta tgtgcccatt gccaggttga
    13021 gtatcttgca tctcaaacag taaaacttga gccgtcaaat tgaacagtca gcggatactt
    13081 catccggtgg ctgttttttg tttttctgaa atatatattt ccacagtttt cttgtatagc
    13141 ttctgtctta gagaagcaag gagctatatt ttgtgctaca atcttaaaaa ggtagagaga
    13201 agaccaaatg gaagacagag gagccactaa aatatgagaa acatcaagtt gacaatcgaa
    13261 tatgacggaa aaagatattc aggatggcaa agacttggag atgacgataa aaccattcaa
    13321 ggaaaaatcg aaaaagtatt gcatcagatg acaaatgaag agatagaaat tattggatca
    13381 ggaagaacag atgctggcac ccatgcgcga ggacaagtag caaattttaa aacaaacaca
    13441 gaaattggac tggcagaaat gatcgacttt atgaatcgtt accttccgcg ggatattgtc
    13501 atcaaaagaa tcgaagaaat gcctgagcgc ttccattcaa gatataatgc ggtgggcaaa
    13561 aaatacagct attatgtttg gaacaacgtg attccttcag catttgagcg aaaccacagc
    13621 ttttatttcc ctcaagagtt ggatatggac aagctgaatg cagcttgcga aaaattagtg
    13681 ggtacacatg atttcattgg tttttctgca ttgaaaaaat caaaaaaatc cacagtgcgt
    13741 acgattgatg aattatctat cgaacgaaag ggagaaatgc ttcattttac ttttgtcgga
    13801 gacggattct tatataaaat ggtgcggatt atcatgggga cattactaga aatcggcaca
    13861 ggtgccatgc cgatagaaac aatcgatgaa atatttgaaa gcaaagtgcg gcaacatgca
    13921 ggagaaaccg tccctgcaca aggtctgttt ttggatgaag tttattatcg ataaaaatac
    13981 tttgataatg gatgaaacgc atgtttcaca gtataaatat cgatgttaaa taatgtatga
    14041 agcaaattaa agaaatataa aaaaagactt tttagcctgt ttatgtgtac agctaaaaag
    14101 tctttcttaa ataaaaatct agtgagaatg ttgtacttct cttctcggtt gtttttgttt
    14161 agaaaaagta aatcgataaa gtaaaaataa aatcaaagta acgatagata aatatcttga
    14221 agcaattgct agttttgtgt actgataagt aataacgact ttatttatac ctggaggaat
    14281 agttaattca gttgtgccaa acttagataa ttttgtttgt actagtttgc cattaataga
    14341 agccacttgc cctttataac gataaatagg tgtcgtcaag acagtatttt tattattcga
    14401 attatctaat tcaattgtaa gtttgttact cataaaatga gttgttggat ttatttcctg
    14461 gttacctagt aaatattgat cattattaat catatcagga tgatagattg actctttgtt
    14521 tgcataatca gtatgattgt aattttcagc tatagcaaca gcgtcttcag gtgctataag
    14581 tgttaatgaa tctttgttag catggataga attgcttact cctgagagat gcaatattag
    14641 tgcaagaaac gtaaagacca ttattttcca tgaattattt ttagtagaaa aaatataata
    14701 gatcactata ctcatcgtat aggctataaa taaagaagaa aaaccattta accgccagac
    14761 aaattgtaga ctcttaactg gtgtatggtt aaaaagttgc caaggaaata aattggttga
    14821 acaaaaaaga acaaatacac caagaaagaa aataaataaa tcatcagcag ttaatttttt
    14881 tataaaaata aaagcaccaa tcaatccgag aaataagaat aatccaatag tatagttgtt
    14941 atagttatta tttaaaatat gtgtcagcat atcactagga gccataccac ttaattcttt
    15001 tcccagtggg actttcaatt cttgtgctct agattgttga ataaaaggaa ttaagaatcc
    15061 cgcacttaaa aaaaaggtga ggacggtagc ttttaaaaga cttaagagtc tagcgatttt
    15121 ttggtcccaa aaataaaaag aaagaaacaa cgttcctcca ataaatacag aaaccatagc
    15181 gacagaaagt aaatgagtat acactactag cgtcatgcca atagacaacc agtaccattt
    15241 ttggtaatct ctaatataaa tttcgtaaca ccccattagt atcaaaggca aaaatgttaa
    15301 agcgacagct tctcccaatg aagcacgatg gaatatgtca atggcacgat aggcggaaaa
    15361 tgtataaata atcgaaaata acagcgaaat ataacgattg ttttttattt gtttcatag
//
LOCUS       DADOOS010000065        15229 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.065, whole genome shotgun sequence.
ACCESSION   DADOOS010000065 DADOOS010000000
VERSION     DADOOS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.065"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="J8C37_001665"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="J8C37_001665"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAZ5305105.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="J8C37_001666"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="J8C37_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAZ5305106.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="J8C37_001667"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="J8C37_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAZ5305107.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="J8C37_001668"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="J8C37_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAZ5305108.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="J8C37_001669"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="J8C37_001669"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAZ5305109.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="J8C37_001670"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="J8C37_001670"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAZ5305110.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="J8C37_001671"
     CDS             complement(8053..8523)
                     /locus_tag="J8C37_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAZ5305111.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="J8C37_001672"
     CDS             complement(8569..8994)
                     /locus_tag="J8C37_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAZ5305112.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="J8C37_001673"
     CDS             complement(9061..9330)
                     /locus_tag="J8C37_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAZ5305113.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="J8C37_001674"
     CDS             complement(9341..10936)
                     /locus_tag="J8C37_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ5305114.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="J8C37_001675"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="J8C37_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAZ5305115.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="J8C37_001676"
     CDS             complement(14489..15049)
                     /locus_tag="J8C37_001676"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAZ5305116.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3949 a         3442 c         2698 g         5140 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaag
//
LOCUS       DADOOS010000066        14815 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.066, whole genome shotgun sequence.
ACCESSION   DADOOS010000066 DADOOS010000000
VERSION     DADOOS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.066"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(296..1024)
                     /locus_tag="J8C37_001677"
     CDS             complement(296..1024)
                     /locus_tag="J8C37_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5305117.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="J8C37_001678"
     CDS             complement(1002..2576)
                     /locus_tag="J8C37_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5305118.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="J8C37_001679"
     CDS             2805..3743
                     /locus_tag="J8C37_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5305119.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="J8C37_001680"
     CDS             3755..4666
                     /locus_tag="J8C37_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5305120.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="J8C37_001681"
     CDS             4690..6162
                     /locus_tag="J8C37_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ5305121.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="J8C37_001682"
     CDS             6213..6824
                     /locus_tag="J8C37_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ5305122.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="J8C37_001683"
     CDS             6854..8767
                     /locus_tag="J8C37_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAZ5305123.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="J8C37_001684"
     CDS             8895..11876
                     /locus_tag="J8C37_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAZ5305124.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="J8C37_001685"
     CDS             11873..13534
                     /locus_tag="J8C37_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAZ5305125.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="J8C37_001686"
     CDS             13574..14506
                     /locus_tag="J8C37_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAZ5305126.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DADOOS010000067        14496 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.067, whole genome shotgun sequence.
ACCESSION   DADOOS010000067 DADOOS010000000
VERSION     DADOOS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.067"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(301..663)
                     /locus_tag="J8C37_001687"
     CDS             complement(301..663)
                     /locus_tag="J8C37_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305127.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(737..1117)
                     /locus_tag="J8C37_001688"
     CDS             complement(737..1117)
                     /locus_tag="J8C37_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAZ5305128.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1199..2374
                     /locus_tag="J8C37_001689"
     CDS             1199..2374
                     /locus_tag="J8C37_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ5305129.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2451..3035)
                     /locus_tag="J8C37_001690"
     CDS             complement(2451..3035)
                     /locus_tag="J8C37_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ5305130.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3236..4234
                     /locus_tag="J8C37_001691"
     CDS             3236..4234
                     /locus_tag="J8C37_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ5305131.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4346..4855
                     /locus_tag="J8C37_001692"
     CDS             4346..4855
                     /locus_tag="J8C37_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAZ5305132.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4885..5544)
                     /locus_tag="J8C37_001693"
     CDS             complement(4885..5544)
                     /locus_tag="J8C37_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAZ5305133.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5546..6310)
                     /locus_tag="J8C37_001694"
     CDS             complement(5546..6310)
                     /locus_tag="J8C37_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAZ5305134.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6318..6995)
                     /locus_tag="J8C37_001695"
     CDS             complement(6318..6995)
                     /locus_tag="J8C37_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAZ5305135.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7032..8417)
                     /locus_tag="J8C37_001696"
     CDS             complement(7032..8417)
                     /locus_tag="J8C37_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAZ5305136.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8386..8640)
                     /locus_tag="J8C37_001697"
     CDS             complement(8386..8640)
                     /locus_tag="J8C37_001697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305137.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8627..8711)
                     /gene="ffs"
                     /locus_tag="J8C37_001698"
     ncRNA           complement(8627..8711)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="J8C37_001698"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8734..9249)
                     /gene="tadA"
                     /locus_tag="J8C37_001699"
     CDS             complement(8734..9249)
                     /gene="tadA"
                     /locus_tag="J8C37_001699"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAZ5305138.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9356..10213
                     /locus_tag="J8C37_001700"
     CDS             9356..10213
                     /locus_tag="J8C37_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAZ5305139.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10294..10629)
                     /locus_tag="J8C37_001701"
     CDS             complement(10294..10629)
                     /locus_tag="J8C37_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305140.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10696..12066)
                     /locus_tag="J8C37_001702"
     CDS             complement(10696..12066)
                     /locus_tag="J8C37_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAZ5305141.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENKEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12144..12680)
                     /locus_tag="J8C37_001703"
     CDS             complement(12144..12680)
                     /locus_tag="J8C37_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5305142.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12989..14470
                     /locus_tag="J8C37_001704"
     CDS             12989..14470
                     /locus_tag="J8C37_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ5305143.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4467 a         2885 c         2354 g         4790 t
ORIGIN      
        1 catgtcaata actatttttt aaatttttta gaaatgtgat ataacatcca cttcagaatc
       61 aacgctgtta aatttatcat gtatttcttc cttggtcaac tataaattaa gcattacttt
      121 gtgaaatgat ttttcgatca attattatta ttcgttatac aaccgttttc aacatatttt
      181 gaaaataaaa tgcactaaaa aactatcttt agcagaacat ttcattgtat tttttggacc
      241 aaacaataaa aaaagaagtt tgaaaaaaat tcaaacttct ttgttcgctt aatctgtatt
      301 ttagaagaaa ggcactccct ctacttcgta agttccaaga acagagcgca tttgacctgg
      361 ttcttcccaa atatatcctt cgcctaattc ttctctttga ttcttttgtt cagtttcaaa
      421 gacaaacaac ggaacatttt cctctttaat atgtgtattt gtcaattcca tcaaactaat
      481 cttgctaaca tcgatatgaa ctatttcttt taagaaatta agtatattcg ctaaaccagt
      541 ctttccttca gaaagttccg ctaacgcaaa ttccaacttg tcatcaattg aatggaccaa
      601 aaatttttta gtaccatcct ccaaatgcaa cataattgta ccagcaactc ttttgcttgc
      661 caaaatttcc acctcgatca acttgttcta tggcttacat aaaaattcta gcacattttc
      721 agaaaaaata cactttttat ttcttatttt ctttccgttc tagttcagat aatgcttctt
      781 ctatccaaat aggtaactgc tccgcaatag tggtaaatcc gatcttacga tttttattcg
      841 tgaaatacct ttttctgcga tatggaacag ctggatttgt tttttctaac gttcttaaag
      901 ataaactgat ttttttggaa tattcatcaa tgtcgatgat ctgaactgtt actggctgtc
      961 cgacttttaa caatgaatgg atatttttcg tataaccgga ttgtacttct gaaacatgaa
     1021 taagtccttg cgtctcgtta tcaagagaaa cgaatgcgcc ataaggctgt atccctgtca
     1081 cttttccttc aattatttgt ccaattttat aagtcatcta cttccctctc ttcgcattta
     1141 tcattatact gtaaatagct caaaaaaaga agcgacgaaa atgactggag gtcttaagat
     1201 gatcgaattt gataaacaga tttcaagaaa agatacagaa agtgtaaaat gggacgcaat
     1261 tgctgagaca tatcaaatgg atgacttact tcctttgtgg gtagcagata tggacttctt
     1321 gtctcccggt ggggtagcta aagcgtttag tgaatacatc aaacatggaa tctttggtta
     1381 tgccactctg tctgataaac tatatcaggc tatcattcat tgggaacgcc aacaccatgc
     1441 tgtggagttg acgaaagaaa atatcgtttt tacaagtggt gtgttaacca gtttagctgc
     1501 agcagtacaa acttttacaa aaccaggcga ttctattttg attcatgacc cagtttatcc
     1561 tccattttcc tctattatag aaacaaatca acgtcacatc gttcgaagct ctttgctaga
     1621 aaaaaataat catttcatca tggacttgtc tgacatggag aaaaaaatcg aagaaaacaa
     1681 tgtaaaagca atgattttat gcaatcctca caatcctgga gggcgtgttt ggagcaaaga
     1741 agaattgaaa cagttaagcg aattgtgttt aaaacatcaa gtgattctgt ttagtgacga
     1801 gatccatcaa gatttaactt tattcgatca tacatttact tccatgctaa cgattgatcc
     1861 taatttagat gatctactag ttgcttttac ttccgcaacc aagactttca atctagctgc
     1921 aatcaaaaat tcaatggtat ttatcaaaaa tccagagatg aaagctgatt ttgaaaaaca
     1981 tttagtgatg aaccaacagc acgaaatcaa cacttttgga ctgattggaa cccaagcggc
     2041 ttatgagacg ggtggagaat ggttaagcca actgattcct tatctagaaa aaaatgttca
     2101 aacagttaat cagttttttg aacagtatct tccgaaagtg agaataatga accctgaagg
     2161 aacttactta atgtggttag attttaccgc ctatatttca gacgaccaag aattggagaa
     2221 cactcttgtt cggaaaggaa aagtcgtatt aaatcctggc atcacgtttg gtccttccgg
     2281 tcatgggcat atgcgcttga atattgcttg tccagaagaa actttactgg aaggacttaa
     2341 tcgaatcaag tatgctttag agacaatcga ttaaatcatt tattcaaaat aaaaggacga
     2401 gaccttacta ggtttcgtcc tttttcataa ttactccgta ccataccaaa ctattctgaa
     2461 atttcaattg tttcaatcac tacatcatgt acaggtcggt cttgtggacc acgttgtaca
     2521 tttgcgattt catctactgt gtccatccct tctagtacat gtccaaagac tgtatgtcgg
     2581 aaatctagcc aaggagttcc gccaccttta tacgcttcaa tgatttctga tgggaatcca
     2641 gcaccttcta gttgtccaag catattagct ggcacatttg tgttatttac aataaagaat
     2701 tggcttccgt ttgtgtttgg acctgaattt gccatagata aagctccgcg aaggttgaac
     2761 agttcacgac tgaattcatc ttcaaaactt tcgccataaa tactttcgcc acccattcct
     2821 gttccagttg gatcgccgcc ttggatcatg aaatcaggaa taacgcggtg aaaaatcacg
     2881 ccatcataat aacctttttt ggaaagttca atgaaatttt gaaccgtctt cggtgctagt
     2941 tcagggaata attgaacagt aatgtcacca cggtttgttt tgataacagc ttttggtcct
     3001 ttttcacttt ttaaatctag ttgtgggaat gccatattgt ttcctcctaa tatttgcata
     3061 cttcataata ataataacat aatacctaac ctctcgtaaa aatactagat ttgttttctt
     3121 ttatttttca tcactagata cattctataa cttaccgttt cgatttcgaa ttcaaacatt
     3181 tttgagaagc attctcaatt aaattctgct atagtgtgaa aagggaggaa tttttatgga
     3241 aatctatgac attacaatca tcggtgcagg tcctgtaggg atgtttgccg ctttttacgc
     3301 aggaatgcgc caagctaaaa caaaaatcat tgacagtcta cctcaacttg gtggtcaatt
     3361 agcaactttg tatcccgaaa aatatattta cgatattcct gggtatccag caataaaagc
     3421 aagtgaatta atcgatcaat tagaaaaaca attaactact tttaaccata cattccattt
     3481 aaaagaagaa gttctctcgc ttactcgtga agacgaagtt atcgagatta ctacaaataa
     3541 aggcatccat tattccaaag cagtcatttt agctttagga aatggttcat tccagccaag
     3601 aaaattgaat ttagataatg ctgaatcatt tgaaaaccac ggattggatt atttcgtgaa
     3661 tgatttgatg aagtatgctg gaaaaaaagt cgctattgct ggaggtggtg attctgctat
     3721 cgattggacg ttgatgttag agcctattgc aagcgaagtt tacttgattc atcgtcgccc
     3781 ggaatttcgt gcgcacgaac atagtgtatc acgcttgaaa tcttcatctg tcaacttgct
     3841 tactccctat ctcatcgatg gattatcagg caataatggg gaactgacag atatccggtt
     3901 aaaaaaagtg aaaagtgacg agacgattga tttgatgatt gattctctga tcgtcaatta
     3961 tggattctct tctaatttag aacatctttc ttcttgggga cttgatagta ccagaaatgc
     4021 cattactgta aaatcagata tgtctacttc tattccaggc gtttatgcag ctggtgacat
     4081 ctgtacctat gaaggaaaag tgaaactgat tgctactggg ctcggtgaag cacctactgc
     4141 agtgaacaac gctttacact atattaatcc aaaggaacga actcagcctg gtcacagcac
     4201 aagcttatac gataaaaatc ttcgtcctag ttgatttttt atatctcttt gcatttcaag
     4261 ttaaacccga aagcagtttc ttgcattttt ttcatgttcc gttaacatga aatagagaaa
     4321 gaaaaaataa agggggagca ataatatggt cgtcaaaact gagactttag aagaaaaggc
     4381 aagagaacta ttgaaggaac gtggcgtaac tgtagaagat atcgcacaac ttgtcatgtt
     4441 tttacaaaaa gactatattt ctgatttgac tttggcggat tgtactgaaa gtgtcaacag
     4501 cgtgctaaca aagcgtgaag tacataacgc cattatcact ggaatccaat tagatatatt
     4561 agcagaaaag aaaaaattga tgagtccgtt gcaacatatt atcgaagatg acgaaggcct
     4621 ttacggtatc gatgagatcc tagccttgtc aatcgtgaat gtttacggta cgatcggctt
     4681 tacgaattat ggttatatcg acaaggtgaa acctggtatt ttaaaagaac taaacagcca
     4741 cgatggcgtt catgtccata ccttcttaga tgatatcgtc ggagcgatcg ccgctgctgc
     4801 tgccagtcga ttagcacaca aagaaccaga aaaaagaagt cgccttactc aataattcaa
     4861 caaaaaaaga aagaaaacgt ctatttagac gagttctttc ttttttgttt gtttcaagct
     4921 attttttcct aagaaaagaa aaacagcaaa aaataattct aataatacaa aaaataagat
     4981 aaagctatgc atgaaaaatt cttctggaag tacattgatg attggatcag taacgggatc
     5041 gaataaccag tcatcgttgt tgaaaaacgt ttcatgaaaa aggataaaaa agcgatcaaa
     5101 tcctatcatc atgaaaaaac cgaaaacaac tggcagtaac atgccgattt gaaacggacg
     5161 aatcaaccgc caaagccgat cattcttttg caaatatttg ataaacaaaa tactcgggat
     5221 caaagtgatg aaaaacacag cataatccac taaaaacaaa attttcactt cataaaagtg
     5281 atgggctccg ctagcagaaa ctgggaaatc tggcaagctg aggatggttt tgaagggatt
     5341 attgagataa ttcattaggt gatcaaaatt tttcagtaac gtctcttgat ctaaagaagt
     5401 ataatccaaa atttgcaaat gtccgatatc aaatacgtat agtggtcgaa agttgatcgt
     5461 cacaccaact gccagactga tcagtgtcag aaataggctg aacaatccag catattccat
     5521 ccactgccat ctcgaatttt tcatattaga agctccattc atccagtgat tgtttcacat
     5581 aggtagctgg gataggcaac ccaggtacat cttcttcttt tgtaaaacct gataaaacga
     5641 gcaaagtatc tatattattg cgtatcccag cttggatatc tgtttcatag ttatctccaa
     5701 ccatgattac ttcttctttt tgcagcccta atacttcaac ggctttatcc ataatgatgg
     5761 cttctggttt tccgatgtag atcggcggcg tttgggttgc tgtctctaca aaagagatga
     5821 ctgaccctgc tccaggaaga aggccgcgtt cagtaggtat attcttatct gggttcgttc
     5881 caatgaaagt agcacctttt tgaatagcaa gtgttgcttt aactactttt tcataggtca
     5941 gataattatc taagcctacc actacatagt caggagcctc ttcttcccat gtaaatccgg
     6001 cagctaaaat caagtcaatc aatcctgctt ctccgataac atagactttt ttcccttttc
     6061 catctgcttt cataaaatcg attgttgcca aagtagctgt gtagactgtc tctggagcta
     6121 catgaatgtc aaactcatct gctagacgat tagctactgt ttctggtgac ttagtcgtgt
     6181 tattggtaac aaacaagaaa ggtaattttc ttttttgcaa ttcttccaca aatcgtttac
     6241 cggccggtat gggttgtgtc ccacgataaa tcgttccgtc taaatcaatt aaatatcctt
     6301 tatagtccat tcatccacta ctcttccctt tttctgatcg taaatgctgt ttttgttttt
     6361 tctacttgtt taggttgttc ttgttttttt actttttcag aggtttttgc tgactctgtt
     6421 tttcgatttt taataaccgg ttttttgcga ttagatgcga ctggtccttt tttctcatcg
     6481 atatacgcac gattatggtt ttttttcttt tttcggcgct gagttgtctt ttctttttta
     6541 ccgccgattc tctcgatgac aaaatacgca caaccaaaat tgcaatattc gtaaagatag
     6601 tcttccaagg aatcgattcg ctgatctgga tgcgcccgtc gattatccgc ttggaaaaat
     6661 ccttttaggc gtagttgttc ataaccccaa tctcctacga tataatcata tcgggctagt
     6721 acatcactgt aacgttctcc aagcttttct gcattaaatc cttcacgata atcggacaca
     6781 agccgataat gtctttcacc tatcaaaaaa ttactgtcat ctaacagtgt cactagctcg
     6841 cctttgactt tttctgcttt aggctcctca acgatttctt ccaaagcagc tgtcagttct
     6901 tcggtgactg ttgtttcctg ttcagctgat gattttttct tagcaatttc tgttttatgc
     6961 tcaacgttct gctgttcatt ttgttttgca gacatgcgtt caccacctat cttttttatt
     7021 ataccttgtt tttatttgat tgggtagtga gctttcaagt aatctcctac cacacttcgg
     7081 ataaattcag ggaacaaaaa ttcatttttt ccagcaatct caatcgttgg aaaaaatgga
     7141 acaaacatca aatgatctac ggtagtaaaa gtataatttt tattcagatc gattggcttg
     7201 ttcttccaaa agacttgatg gttaatagaa tcatatgtga ttccattata gaccatctgc
     7261 ccaaaaatct taccgcgaaa tcccattccc ttcataggat agttacgtaa aaagttacga
     7321 tttttttcta tttctaaaac taagcggatc agatcatttc cacccaatgt gacatttaac
     7381 aagtgcatcg gatgcggcaa cgcagtgtga agctgatcct gattgacccg cccttttgga
     7441 atttcagtta aaaacagacc actgtttaat attgctgctt ccgtctttcc tcgttctttc
     7501 accgcctcaa aggctacttg gataaacgaa tgctcattga agatatctag actcagagaa
     7561 aatggcaaat ctgctacttc tttttctttt aataattcat gcccacgttc tagataacta
     7621 tttatttctt gttcatcttc tgtaaaagct gtcattgttt cggtagggat ggctttggct
     7681 gaatgcttaa ggattttttt ctcttcatcg atggttaaat ggacttcacc gacatactgc
     7741 ccgtactttc cagcggctgc tatttggaca tcattgatta cgtgaccttc tctgaataaa
     7801 tgatgggtgt gcgatcctaa aataacatca attgctggta tttcttttgc aatttggaaa
     7861 tcatcctgaa tcccgagatg actcatcaaa actaaaacat ccacttgtga acgtaaatct
     7921 tcgactaatt ctggcaaaat atcataagga ttccgtatgt cccagccatt cggattataa
     7981 gtaagcggaa atggagcagt aaatgcaatt agtccgattt tcgtctggtg tttcgttgta
     8041 atgattttat atggtttcgt ccatgccggt ggctggagcg tatttttatc gaataagtta
     8101 tccaggatga tatcaaactc agcatgatcg tataaatgat tcaactcttc tttggaattt
     8161 cctacgcctt cattgtttcc tatcgtcgct gcatcataac caacttcatt catcagcaag
     8221 atattttcct gtccattcgt tgcttctgac aatggatgcc aacgatccac aaaatcacct
     8281 agatctactg caatcgttgt cgttttttct ttcttttcgt tttctcgttt acgttgatcc
     8341 aaaaaccttc tgatccttgg ccaattttct aaatgagaat gcagatcatt cgtatgtaaa
     8401 atcactattt tttccatgta ccctctcgcc tctctttatc tttatcatac cataggcagc
     8461 tttttcttcc attgaaacaa gctctatttc agagtttttc ctgccttgaa gcaaattgta
     8521 taagtacaat attcgataaa tcagtctggc taaaaaatca ggaacattca tttttacagt
     8581 actataaaaa taaaaaaaac agacccgaaa gtctgttgtc gtttcattat aatcagacac
     8641 atggcggaac ctgcttagtg ctgcttcctt ccagacctga cacgcttcac aagcccacca
     8701 ttgtcctagc ctttcggcaa agaatagtat accttatttt tcattcgttg accagtcttt
     8761 ttttaatttc ttttttcgct ggcggagctt tcggaaaaag tcagtcaaaa tcatcccaca
     8821 ttcgtcttct aaaatgccac cttctacata tgcttggtgg ttaaaccgct cgtcctccaa
     8881 aagattcatc aaggtaccgg ctgttccgcc ttttggatca tacgcgccga aataaacttc
     8941 tgctacacga gacaaaatca tcgcgccgct gcacatagga caaggctcta aggtaacaaa
     9001 aagtgcagca tcttccaagc gccagcttcc cactttttca caggcttcct tgattgcaag
     9061 catttctgca tgtgtagtag catcttgcgt ggtttctcta aggttatatc ctcttccaat
     9121 cacttttcca tccaatacaa ccactgctcc aatcggaact tcatgaagca tttctgcttt
     9181 ctttgcttct gccaatgcaa ggcgcatcca tttttctttt tctactgcat caagatttgt
     9241 atttaccaat tacccttccc tgcctttgtt ttttcttgtt ttactttagc atatgataaa
     9301 aaggctgtaa ctaacttttt tttctatggt aaaataaata caagaggtga acaagatgaa
     9361 aatggataaa ttacttgctc tttacccaca agcacagaaa aaaaactctc cagcatcaga
     9421 agcggatatc ctttcttttg caattgatga tgcttttatc tggatcaaac aagattcatt
     9481 atctcaacaa gaaaccagcc ttttgaaagc gttattccct gtaatcaacg atcaaaaaaa
     9541 acacccatgg tatcgctatc tttttaaaga tgcttcttgt gttcaagaaa aaagctttcg
     9601 tttgatccag cttcacgtcg aatcaaaagg agaattctta aaaaaagaat ggcaagatac
     9661 tattttggaa ttatttcgct attctgctga tttctttttt tatacagaca cagatgcgct
     9721 cttagtcgaa caaataaacc gtgattatct tgatgctact gaaatcaacg gtatttttct
     9781 atctttagat gctgattttg atacgacaac gtctgtattc gttggttctt tccattcacc
     9841 tggcaccgac ctcgtccctt tgtttgctga agaaagaaaa atttttttaa cagagcaaaa
     9901 caatctttat actcacagcc gtactttttc tatgcaagat gtggctttac attactatac
     9961 aaaagatact gtaaaagata gtaagttgat caaaagttac atagaactga tcaaacaaaa
    10021 tgaaatggaa gtgatcattc aagaattatg gaatgaatta ggcaatgtca gctctgctgc
    10081 aaaaacactg tttcttcatc gtaatactct aaaatatcga atcgaaaaat ttcaagaaca
    10141 gtctggtttc aatctaaaaa aagccaacga tttactgttt tgctatcttt tattgcttca
    10201 tgaacataat taaagataga ttaaaggggt tgtgacaata attttgtcac aaccccttat
    10261 ttcgaatgaa tagtgttcaa aagctagttt ctcttacatc attttttcaa caaaatgcat
    10321 tagccggtct tttacatctt cggttgaatc tttgattgaa tcaccataaa cagagatttt
    10381 cttttttcct gatttgattt ttgctaacat tcccatcaaa gaatccaagt cactatctga
    10441 aagaccatct acgatttcta gtaatttttc atttccatca aatttgtcat agacgaattt
    10501 ttttactttc gtccgagtag tcatatgatg gatcttttcc attacttttc ccgatacaac
    10561 gactgctgtc gctacactgg cagcggctgc tacgcttaat ccgattgtta cttttgttga
    10621 tgttttcata agtatcagct cctttttatc tatctccatc atagcataga caaaaaaata
    10681 aacgaactaa tccactcagt gaaatctcaa tcctttaggg aaaaagttct ttactgttcc
    10741 ttttacttgt ttcccaaatc cgacaggtat cttatctaat actagcaaaa cccatccttg
    10801 attcccatca cgttgaaatg tttctcctgc tgtgtaagat tgccattcct tttgagtgat
    10861 cggcaagcaa ggtatattct ctatcttttt tgtagcaagc gctaatgcat agctaggttc
    10921 aaatcgattc tttttgaaat ctcctaaatg caaacctgta cgtacgactt ttaaaccatc
    10981 gagagaaggg gctaactcag gaacttccca aagatggtcg ttgaacacta aaagtcttcc
    11041 agtagcttca taatgaaaat catttgaaaa ctccgtccag agtttttcct gctcttttgt
    11101 catttgcact ttactttttt ttcgtgtctt cttttcttta tgcatctgat tttgtccatg
    11161 aaaagtcagc tttgcaacaa aatgtccttc accctgatct ttatgaggcc atatacgtat
    11221 tgttttttcc aaaccagcta cgcttcccca ttcagaacgt cctgaactaa cggattgagt
    11281 caaaggaatc tcctcaatcg ttactggata attttctaca agccagctaa tgatctcttt
    11341 attttcttct ggtgcaaacg tacaagtcga ataaatcagt tgtcctttat tttttagcat
    11401 ttttattgct gaagacaaaa tctcttgctg acgtttttga cagtatagcg gagattcttc
    11461 tgtccactct tttattgcat ttggatcttt tcggaacatt ccttctcctg aacaaggagc
    11521 atcaaccacg atccgatcaa aaaagcccga aaaatgaggg acaagttctg cgggtgcatg
    11581 atttgtcact attgcgttcg aaactcccca tcgttctata ttttctgata aaatcttcgc
    11641 acgtttcgga aagatctcat tcgtcactaa taaccctttc cccttcattt gagcagctaa
    11701 ttgggttgat ttaccaccgg gagccgcaca caaatccaaa actttttcac cgggttttgc
    11761 tgcagcagct gttccaacga tcattgcact tggttcttgg ctatattcat acccagcttg
    11821 atggaggaaa gactttccat tgacagtgcc aagaaatccc tcattgctgt agggagcggg
    11881 ttgtaattct tcgctatggt aagtttggac catgtccaat cctgccggtt ttagtggatt
    11941 ccaccgaaat ccttttttca cgcttccttg ttcaagcgca gaaaaaaaat cggatgcttc
    12001 ttcgcctaaa agaagacgat attttttgat aaattgctgt ggtaacgttg tcgcttcctc
    12061 cttcattcca atgtttcgtt agacatttta caatcttttt tacgaaaaat aaaggtggct
    12121 gatctacttt ttcatgttgc tgcctaaggt gactgttcct ttaagatatt ttccaagtat
    12181 tcatccgtca cctcgttttt atcccaaaca gcgttcatca tttcaagaaa atcttgagaa
    12241 gaatcatctg agcgcaaact tcccaatgca ttaagccata ccaattcaag cgacttttct
    12301 tctttgatct tcgtgtgacc tttgacatat gagaaaaatg gccgcttaaa tagcgaatcc
    12361 atgtctaaat cagattttgc aatgatttca cgaatacttt catggataga aaaaaaatct
    12421 ttcaataagt agacaatgct taaaacatca tcggaactgc attcagctaa gtagttttca
    12481 aaataaacag tgatttcttc taatatcttc tcttttaacc cgctgattga tttgaaatgt
    12541 gtataaatcg ggtatgtaga acaaccgcag aactctgcta actctcttgc agtaattgac
    12601 ttcattccat attcttccac gtataccata cttttgaaca gcaaatattc tttactataa
    12661 tttgttactc tccccatcat tcttacctcc gtctcttttc atctacccat aaataaataa
    12721 cataaataat tctaaaaata ttattgataa ttatcaaaac atatttagtt atatccatta
    12781 aaaatcaatt tttccgcctt aaaaacaaga atatcttgac aaaataccct aggaaataaa
    12841 caatagaata aacaaggtaa caaaaaaatt aaataacgtt atataaaaaa gaaggcgaca
    12901 ctctgtgaga ataacacaga ctgaaatgac tttccactca cagtttttgt ctaactatat
    12961 acaaaaacag aataaaaagg aggtatttat gctaattgaa gcaagaaaaa aagagttact
    13021 tgttctacaa atggtcattc aaaaaaataa ttgttcttta caagaattat ccaatgactt
    13081 acatattcca aaacggacaa tcaaagaact gatccgaaaa ataaatgtat cactcaagca
    13141 atttttattg attgatgaat ttatttattc taatcataaa ggagagatcc aaaccaaaga
    13201 aaccgatcaa actaaaaagt tagctgtttt ttctaagtta aaattatgct acctaaaaga
    13261 atccaatcgt tttaactatt tgatgttact aatcaattat ccacaaactg ctgtaccaaa
    13321 aaaatattta cttgaacaac tttatatatc gccctcctat ctcgaaaaac tgacaaaaca
    13381 gctcaatcat tccttaaaaa aattccattt acggatccta tctgtaaaaa attgctgtac
    13441 tcttgaagga aatgagttat ctatccgtct ctatctttac ttttttcttt cagatacatt
    13501 tggaggtaac gattggccat tttcctcact tgaattaaat gcactaaaaa tgaacagtac
    13561 agatgagcaa atagaaaaaa tttcaaaaca agaaaaaaat atctacttgc tgttaacttt
    13621 attcatatta cgaaacaaac aacaggcata ttttccaaaa acttcttctg aaatcactga
    13681 attgatggac ttattgcagc atacacatga ttattcagaa tactttactt ctttttcagt
    13741 gagtcgccct tgtgaagaga cagaaacaga gagagcctac tttaattatt ttttctattg
    13801 tttttttcca aatcaaatct cttttcgaca aaaacaggag attggttttt tattttccac
    13861 aagcaaaaac cttatttgtc gacaagtcgg tcaattgatg acttacttaa ctcaacagtt
    13921 tcctctcaac catttagaaa ataaaatacc aatttatttt tattttcttg tgttggcagt
    13981 tgtttttttc gaactgttgg gtgaatccag cagtagtttt tctacacttt gctttccaga
    14041 agccacgtcc aatcattcta gttcttttat acaggaaaag aaattaagag aaaaaatcga
    14101 tacaattttt tcaaaaaatg gggaaagaaa ccaaatcagt caatacctta gtcaattgat
    14161 ccaacactta ctacaaacag aaacgaaaga gcagttactc atccatttgc agataaccaa
    14221 acagtttaca ggaaattatc atcttcaaag ccatttgcag cagttatttc gcaaagatgc
    14281 tgttaaaatt accgcatatt cagaacatgc tgatttgatc attacagatc agcatgagaa
    14341 aatttcagat gaaacggaga ctttctatct ggatacaacg cacaatacag tgttatttgg
    14401 caagttaata aaagcggttc atgaactgta tatgaaaaaa ggttatgaga acaaaacaaa
    14461 gaggatgtga caaaattctt gtcacatcct cttatt
//
LOCUS       DADOOS010000068        14107 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.068, whole genome shotgun sequence.
ACCESSION   DADOOS010000068 DADOOS010000000
VERSION     DADOOS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.068"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            66..767
                     /locus_tag="J8C37_001705"
     CDS             66..767
                     /locus_tag="J8C37_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAZ5305144.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="J8C37_001706"
     CDS             764..1885
                     /locus_tag="J8C37_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAZ5305145.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="J8C37_001707"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="J8C37_001707"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAZ5305146.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="J8C37_001708"
     CDS             3264..4172
                     /locus_tag="J8C37_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAZ5305147.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="J8C37_001709"
     CDS             4207..4479
                     /locus_tag="J8C37_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAZ5305148.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="J8C37_001710"
     CDS             4490..5536
                     /locus_tag="J8C37_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAZ5305149.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="J8C37_001711"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="J8C37_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAZ5305150.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="J8C37_001712"
     CDS             complement(8547..9218)
                     /locus_tag="J8C37_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305151.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="J8C37_001713"
     CDS             complement(9211..9705)
                     /locus_tag="J8C37_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAZ5305152.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="J8C37_001714"
     CDS             complement(9725..10945)
                     /locus_tag="J8C37_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305153.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="J8C37_001715"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="J8C37_001715"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAZ5305154.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="J8C37_001716"
     CDS             complement(13021..14103)
                     /locus_tag="J8C37_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5305155.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DADOOS010000069        13932 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.069, whole genome shotgun sequence.
ACCESSION   DADOOS010000069 DADOOS010000000
VERSION     DADOOS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.069"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(30..830)
                     /locus_tag="J8C37_001717"
     CDS             complement(30..830)
                     /locus_tag="J8C37_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAZ5305156.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(827..1639)
                     /locus_tag="J8C37_001718"
     CDS             complement(827..1639)
                     /locus_tag="J8C37_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAZ5305157.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(1834..2391)
                     /gene="frr"
                     /locus_tag="J8C37_001719"
     CDS             complement(1834..2391)
                     /gene="frr"
                     /locus_tag="J8C37_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAZ5305158.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(2394..3116)
                     /locus_tag="J8C37_001720"
     CDS             complement(2394..3116)
                     /locus_tag="J8C37_001720"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAZ5305159.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(3252..4133)
                     /locus_tag="J8C37_001721"
     CDS             complement(3252..4133)
                     /locus_tag="J8C37_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAZ5305160.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="J8C37_001722"
     CDS             complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="J8C37_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAZ5305161.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            5373..5852
                     /locus_tag="J8C37_001723"
     CDS             5373..5852
                     /locus_tag="J8C37_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAZ5305162.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            6069..7160
                     /gene="phnW"
                     /locus_tag="J8C37_001724"
     CDS             6069..7160
                     /gene="phnW"
                     /locus_tag="J8C37_001724"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAZ5305163.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            7153..7281
                     /locus_tag="J8C37_001725"
     CDS             7153..7281
                     /locus_tag="J8C37_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAZ5305164.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            7285..7830
                     /locus_tag="J8C37_001726"
     CDS             7285..7830
                     /locus_tag="J8C37_001726"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAZ5305165.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(8284..9975)
                     /locus_tag="J8C37_001727"
     CDS             complement(8284..9975)
                     /locus_tag="J8C37_001727"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAZ5305166.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="J8C37_001728"
     CDS             complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="J8C37_001728"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAZ5305167.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(11457..12476)
                     /gene="argF"
                     /locus_tag="J8C37_001729"
     CDS             complement(11457..12476)
                     /gene="argF"
                     /locus_tag="J8C37_001729"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAZ5305168.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="J8C37_001730"
     CDS             complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="J8C37_001730"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAZ5305169.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3879 a         2920 c         2474 g         4659 t
ORIGIN      
        1 aaattcacaa agaaacgcag gtatacttac taaataatgc caaataaatg catgatagga
       61 aatacaaaca gcaaactgtc gaaacggtct aagattccac catggcctgg taagatattt
      121 cccgagtctt tcacatcata atgacgtttg attgcagatt cgaccaaatc accaaattgt
      181 ccgactgctg aaaaaataat cgtcatcaga agcatgacaa acataccatg attaaatagt
      241 tcttttgctg ggtatagcaa taaataaaga aatgcgacta caacagcact accaatacca
      301 ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag gacttaactt atgctgacca
      361 taacgtcgac caaccatata tgctccgata tcagttgccc aaacaataaa tagaccaaat
      421 atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat tctggaaacc tattcccaca
      481 tacaagctga caagcacagg aaaacctgct tcatcaatcg tatacgtatt tttagaaata
      541 actgatgttc ctaaaagaat cataactata agataaaata acataaatgt acttacattc
      601 tctggaagaa agaaaaacca tctttctttt ggcaaaacaa gtaacactgc accaacggcg
      661 gataaaactc cttcaaaact taagagtgtc aatcctttca tccgaaacag ctcgtaaact
      721 cctattactg ctaagattgc tgctgtaagc tcaaccgcta tgcctccgat ccatataatc
      781 ggaataaaca agactagcgc cacggctgct gtgaccacac gttgtttcat gactgatccc
      841 ctttctcatc ttctgttttt tttacaccgc cgaatcgacg gtctcgattt tgataagagg
      901 cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca aagcgctttt gtaaaataca
      961 gctcactata tgcgatctgc cataacagaa aattactaat tcgctcttct ccacttgtac
     1021 ggatcatcag ctcaggatct tgtaactctt taggcaagaa acctgtcatt aaatgcttag
     1081 caatcaattc atcatcgatt tcttctgcgt gtatttctcc ttttgctact tcttcggcaa
     1141 tttcttggac agcagtcacg atctctgctc tgcttccata attcaaagca aaattcaata
     1201 ccattccagt attgtttttt gtttgctcaa ttgcacgtct tacagcatct tgcgtatgtt
     1261 ctggcaatac gttttcataa cccatgacat gaactttgac attttcttta atcaattctg
     1321 gtacgaacgt atcaaagaag tcgacaggta gttgcatgag aaaacttact tcatcttttg
     1381 gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa cactttcact ccaagccttg
     1441 atgctttttt tgtgactttc tttactgttt ccatgccttc tttatggcca gcaactcgtg
     1501 gcaaacgacg gttttgcgcc caacgaccat tgccgtccat gatgatggca atgtgctgag
     1561 gaattggtcc atcggtatta aattgaaatt cgcttttttc ttgaatatat ttgttttttt
     1621 gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa aagattccca ctatcttaag
     1681 tattatagca atatctaact gtttttccta tgaaatcatt gtttttttat ttaaaaactg
     1741 aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa cagaggaagc
     1801 ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt
     1861 atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt gtacgtcttt
     1921 ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc
     1981 atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt
     2041 aacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat
     2101 gacatttcca tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac
     2161 atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg
     2221 gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc
     2281 caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg
     2341 tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc
     2401 ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca
     2461 tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa
     2521 tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact
     2581 gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc
     2641 aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag
     2701 tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct
     2761 gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt
     2821 gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct
     2881 aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc gatttgtccg
     2941 cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc gtgaacttct
     3001 ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcgtcccca
     3061 gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc
     3121 attcctccaa tattttctat tgccatttta ccatacttag atgatttcga cactctaaga
     3181 ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt
     3241 attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt
     3301 ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat
     3361 gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg
     3421 atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt
     3481 ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct
     3541 ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct
     3601 cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc
     3661 atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg
     3721 aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct
     3781 ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt
     3841 tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct
     3901 gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca
     3961 gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca
     4021 actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct
     4081 acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac
     4141 tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc
     4201 ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa
     4261 caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa
     4321 ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca
     4381 atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat
     4441 cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag
     4501 caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag
     4561 cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt
     4621 ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc
     4681 tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa
     4741 cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg
     4801 tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg
     4861 catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga
     4921 agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg
     4981 cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat
     5041 ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc
     5101 aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat
     5161 ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata
     5221 aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa
     5281 aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat
     5341 acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga
     5401 ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg
     5461 ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa
     5521 tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa
     5581 ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat
     5641 tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt
     5701 tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat
     5761 tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc
     5821 ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg
     5881 cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc tttacaaaaa
     5941 tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta
     6001 tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag
     6061 gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa
     6121 atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga
     6181 taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa
     6241 tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag
     6301 atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat
     6361 gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg
     6421 tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg
     6481 agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag
     6541 atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg
     6601 aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct
     6661 tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt
     6721 tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt
     6781 tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag
     6841 aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa
     6901 aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct
     6961 cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc
     7021 aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa
     7081 atattggcga gatctatccg gaagatattt tggaactgac agcaattatc aaagactata
     7141 aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt
     7201 ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg
     7261 actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt
     7321 ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa
     7381 aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag
     7441 cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc
     7501 aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca
     7561 aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt
     7621 cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat
     7681 gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg
     7741 ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa
     7801 aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag
     7861 ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg
     7921 acatgaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc
     7981 aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat
     8041 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac
     8101 gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac
     8161 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
     8221 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
     8281 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
     8341 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
     8401 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
     8461 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
     8521 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
     8581 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
     8641 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
     8701 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
     8761 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
     8821 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
     8881 gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa
     8941 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
     9001 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
     9061 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
     9121 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
     9181 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
     9241 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
     9301 agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
     9361 tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg
     9421 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
     9481 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
     9541 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
     9601 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc
     9661 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
     9721 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
     9781 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
     9841 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
     9901 ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
     9961 atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa
    10021 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
    10081 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
    10141 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
    10201 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
    10261 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaacaatc
    10321 cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg
    10381 aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc
    10441 tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca
    10501 atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat
    10561 tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg
    10621 acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct
    10681 aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct
    10741 gtttctacaa caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc
    10801 agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt
    10861 ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct
    10921 ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga
    10981 tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt
    11041 tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt
    11101 gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga
    11161 atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt
    11221 tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca
    11281 cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc
    11341 atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt
    11401 tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa
    11461 acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata
    11521 cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact
    11581 tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca
    11641 tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc
    11701 atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct
    11761 tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca
    11821 acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg
    11881 acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa
    11941 attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca
    12001 acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt
    12061 tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct
    12121 agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct
    12181 agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct
    12241 agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt
    12301 gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata
    12361 ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct
    12421 tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc tttcatatta
    12481 aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga
    12541 cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc
    12601 atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg
    12661 atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca
    12721 cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc
    12781 tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc
    12841 gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta
    12901 aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga
    12961 tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt
    13021 gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa
    13081 gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat
    13141 tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc gattccttta
    13201 tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt
    13261 gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt
    13321 gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga
    13381 tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta
    13441 tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca
    13501 ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct
    13561 gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc
    13621 aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg
    13681 aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc
    13741 agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg
    13801 tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat
    13861 aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat taaataatta
    13921 aacgtttttc ta
//
LOCUS       DADOOS010000070        13734 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.070, whole genome shotgun sequence.
ACCESSION   DADOOS010000070 DADOOS010000000
VERSION     DADOOS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.070"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(140..1342)
                     /locus_tag="J8C37_001731"
     CDS             complement(140..1342)
                     /locus_tag="J8C37_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5305170.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1345..1539)
                     /locus_tag="J8C37_001732"
     CDS             complement(1345..1539)
                     /locus_tag="J8C37_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ5305171.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1586..2434)
                     /locus_tag="J8C37_001733"
     CDS             complement(1586..2434)
                     /locus_tag="J8C37_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAZ5305172.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2639..2989)
                     /locus_tag="J8C37_001734"
     CDS             complement(2639..2989)
                     /locus_tag="J8C37_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305173.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3584..4030
                     /locus_tag="J8C37_001735"
     CDS             3584..4030
                     /locus_tag="J8C37_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAZ5305174.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4654..5646
                     /locus_tag="J8C37_001736"
     CDS             4654..5646
                     /locus_tag="J8C37_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5305175.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5662..6546
                     /locus_tag="J8C37_001737"
     CDS             5662..6546
                     /locus_tag="J8C37_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5305176.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6595..8202
                     /locus_tag="J8C37_001738"
     CDS             6595..8202
                     /locus_tag="J8C37_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ5305177.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8203..9687
                     /locus_tag="J8C37_001739"
     CDS             8203..9687
                     /locus_tag="J8C37_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAZ5305178.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9864..10856
                     /locus_tag="J8C37_001740"
     CDS             9864..10856
                     /locus_tag="J8C37_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5305179.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10908..11777
                     /locus_tag="J8C37_001741"
     CDS             10908..11777
                     /locus_tag="J8C37_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5305180.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12197..13690)
                     /gene="guaA"
                     /locus_tag="J8C37_001742"
     CDS             complement(12197..13690)
                     /gene="guaA"
                     /locus_tag="J8C37_001742"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAZ5305181.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4214 a         2323 c         2833 g         4364 t
ORIGIN      
        1 aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta ttcccactca
       61 atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta ttcctcattt
      121 tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag tttgcaactt
      181 tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca attccttcat
      241 cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta gaatggccta
      301 accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag ctaacatgag
      361 aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca taacgctgaa
      421 taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga ataaattgtt
      481 ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt gtatcatcgt
      541 ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga ataaaatctt
      601 tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg aagtccacgt
      661 cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa tatagccaga
      721 tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc aaaacttctt
      781 caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt gcttttgttc
      841 ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat gatttacgaa
      901 acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc aataaccaaa
      961 ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca ccgaaaaatt
     1021 cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt acgctagctt
     1081 tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca taattcgcaa
     1141 atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt gcttcacgag
     1201 ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc ctctttccag
     1261 tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta tcttgataca
     1321 cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac ctataatttc
     1381 ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg gatatccctg
     1441 atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg ttttaaatcc
     1501 tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc actttcaaag
     1561 aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga acggtacttt
     1621 cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa gcattgtaaa
     1681 aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc gtggaatcat
     1741 agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg gacttgaaaa
     1801 aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg ctgatatagc
     1861 gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa ccctgacccc
     1921 aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata atatggtagt
     1981 ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt gcttttttag
     2041 ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta tttgcatact
     2101 caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga ttgtcaaaat
     2161 tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc tgtttgcgtt
     2221 ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt ttttcaagag
     2281 taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt acttcgtttt
     2341 cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca atatacgtgg
     2401 gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg ttgcgtattc
     2461 gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag ttaaggggga
     2521 aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac ggacagaaac
     2581 ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca tagtatagtt
     2641 agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca gcggttgccg
     2701 ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc accaaattgg
     2761 taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc acaggatgtt
     2821 cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata cattcgtagc
     2881 tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact tggtattctt
     2941 tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc acaattctcc
     3001 ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat ctacgacttg
     3061 tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat atacgatttt
     3121 ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt ttggtttagt
     3181 acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg gaatgtctct
     3241 ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca gttcctacgt
     3301 tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta tcagcggaaa
     3361 acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt atttgtttct
     3421 cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa taaagcaaga
     3481 attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct atcctttgga
     3541 ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg actatgtcgt
     3601 acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat catcaaaagc
     3661 aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg agatgagtgg
     3721 tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat caataaaaac
     3781 ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa agttgatcaa
     3841 tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa tgatatttac
     3901 tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat ttcaatcgat
     3961 gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt atgattggtt
     4021 tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat ttttttcagc
     4081 ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa gtactcaaga
     4141 gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa tgtactcttt
     4201 tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga aaacatattg
     4261 attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac tggtgagtgg
     4321 tcttgaacat acttttaaat cacattttac tgagttttat tggattttat aattcattgg
     4381 aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat tccagctatc
     4441 aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat tattatcaga
     4501 atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa aaaatggata
     4561 tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg ttaaattttt
     4621 atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat tgaattggaa
     4681 aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa aaatatttcg
     4741 aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt gttgctctta
     4801 ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc tgtgattgca
     4861 tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg actagatcac
     4921 tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac attaaaaatt
     4981 agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact gagcttgaat
     5041 caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc gccgaatttt
     5101 atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa tggacctatt
     5161 aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa tccagcttat
     5221 ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc atccattctt
     5281 tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa tatcgatggg
     5341 gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc tgtgatggtg
     5401 atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa agcctacttg
     5461 atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt atacaaagtc
     5521 ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa tatgattatt
     5581 aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga tggtcaaggc
     5641 ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg tcaaatctta
     5701 tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc gctgatttat
     5761 gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat aagcttcaat
     5821 ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc cattttacgc
     5881 ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt atttattacg
     5941 atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat tattatgact
     6001 ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt attggtaaag
     6061 aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat aaatgtgtgg
     6121 aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc tgaagctgct
     6181 actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc attagcgaaa
     6241 ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc atactttgat
     6301 gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt aagaaatatt
     6361 ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat ggcagaaatg
     6421 aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct gccattaatc
     6481 ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg ttctgttaaa
     6541 gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt taagatgaaa
     6601 gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc ttgtggaggc
     6661 tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc ttttccattg
     6721 aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc caatgatcct
     6781 aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat tgattggaca
     6841 aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg tgatttacca
     6901 gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc ggaaagtggc
     6961 gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa gaaaattttt
     7021 gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat ttactctatg
     7081 ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa tgggatggct
     7141 tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac cactgatcag
     7201 ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg tgaagcagat
     7261 gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt tttaatgggg
     7321 gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg tcaggtacaa
     7381 tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga aatgaataat
     7441 aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt tgccaaaggg
     7501 aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac gggtgcagat
     7561 gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca tgttactcgt
     7621 acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa taaaaacttg
     7681 gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt ccaaaataac
     7741 tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga tggtatgtta
     7801 aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac cgaagctgga
     7861 ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc agatgatgcg
     7921 aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga agacaatatc
     7981 tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat cgaaacggat
     8041 atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa aatcgatgaa
     8101 gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg gattcaaata
     8161 aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt agaagaaacg
     8221 gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt taaccctcag
     8281 tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc caatgggttt
     8341 atatattaca aagggcatta ccatctcttc tatcaatact atccttacga tagtcgatgg
     8401 gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga agatctacca
     8461 gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg aagtgcaatt
     8521 gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga tggtattagc
     8581 catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa atacagtggt
     8641 aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga atttcgtgat
     8701 cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa atctacacag
     8761 tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca cttttactct
     8821 gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga tttattccat
     8881 ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa cggactggaa
     8941 tatcataata tcagttctac catggcttgt attggtgagg taaactggga aacaggtcag
     9001 atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc accacaaaca
     9061 acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg ggatcgtaca
     9121 ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc ccgagagtta
     9181 agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag tcatttagtt
     9241 tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt tcgaaacgcg
     9301 ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga agtttttaca
     9361 attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac tgctacacag
     9421 gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga agaagaccct
     9481 ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct tgagattttt
     9541 agagatacct catcaattga agtctttatt aatgggttag aagttatgac ggctactttc
     9601 tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag tcgtattcaa
     9661 gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga atcaattatt
     9721 taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt tcccatggat
     9781 ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat actaaacttg
     9841 tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca aaattagcag
     9901 gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa gcttctacta
     9961 gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt tatgcccggg
    10021 ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta tggcatccat
    10081 tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac tacaagcttt
    10141 atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag atggtcagcc
    10201 aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa tacatttcga
    10261 gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt tatgtgaccg
    10321 ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga ggatgtcaga
    10381 atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt cgtcgaaaat
    10441 attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat atgttagaac
    10501 ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc gatatcgatg
    10561 ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt gaaaaaatag
    10621 ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag atgacagaag
    10681 gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg gaagtggcag
    10741 tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc gtattgccga
    10801 ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt gcttagtgca
    10861 aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg ttatatggtg
    10921 gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa ttacaagtga
    10981 tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa gtgtttgatt
    11041 tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt gggcctattg
    11101 atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag ttagcttggc
    11161 aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc tattggacta
    11221 ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa ggtaaaaata
    11281 gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg gatgggaaaa
    11341 tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt cataatgaag
    11401 atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta gcatcaggac
    11461 ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat catccttatt
    11521 ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta ctatatgcac
    11581 cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc gagttgattc
    11641 gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc gaagaatatc
    11701 tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct ttagcaataa
    11761 agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc ggaagtttaa
    11821 aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga atctgagtag
    11881 agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta caagaaagtt
    11941 ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt actaaaaatc
    12001 tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa gcaaagccct
    12061 tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac catcttattt
    12121 tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta ttcctcatcc
    12181 atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg gtaatatcat
    12241 acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt ttttgcaaga
    12301 cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg acagcacgga
    12361 tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag cgaatgcctg
    12421 gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg atttcttcgc
    12481 gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat tcaccaagga
    12541 cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg tcaggcatgc
    12601 ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat ggctcgatca
    12661 gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg atggtttggg
    12721 cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca aggaaagaaa
    12781 cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca aattcatttc
    12841 caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat aagaaacgtt
    12901 ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt tccataactt
    12961 gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt aattgatcgc
    13021 cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct gaaaggccta
    13081 ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg tcgatgaagt
    13141 tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg cgcaacaagt
    13201 catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac attttgcgtt
    13261 ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca tcgaaatcag
    13321 tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt tcaggagtgt
    13381 tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg tattcacggt
    13441 ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata ccgtaacaga
    13501 ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag gccttctcat
    13561 cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt gcctttactt
    13621 cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca cgaatacggc
    13681 gagtgatcag ttggttatac tgactaccgt aatcaagtac gataattttt ggct
//
LOCUS       DADOOS010000071        13404 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.071, whole genome shotgun sequence.
ACCESSION   DADOOS010000071 DADOOS010000000
VERSION     DADOOS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.071"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            79..1125
                     /locus_tag="J8C37_001743"
     CDS             79..1125
                     /locus_tag="J8C37_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAZ5305182.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="J8C37_001744"
     CDS             1474..2106
                     /locus_tag="J8C37_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAZ5305183.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="J8C37_001745"
     CDS             2694..4133
                     /locus_tag="J8C37_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5305184.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="J8C37_001746"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="J8C37_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="J8C37_001747"
     CDS             4895..5110
                     /locus_tag="J8C37_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305185.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="J8C37_001748"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="J8C37_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="J8C37_001749"
     CDS             complement(5770..6681)
                     /locus_tag="J8C37_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305186.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="J8C37_001750"
     CDS             complement(6700..7704)
                     /locus_tag="J8C37_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAZ5305187.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="J8C37_001751"
     CDS             complement(7701..9824)
                     /locus_tag="J8C37_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ5305188.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="J8C37_001752"
     CDS             complement(9829..12276)
                     /locus_tag="J8C37_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ5305189.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="J8C37_001753"
     CDS             complement(12263..12652)
                     /locus_tag="J8C37_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305190.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="J8C37_001754"
     CDS             complement(12712..13215)
                     /locus_tag="J8C37_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ5305191.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="J8C37_001755"
     CDS             complement(13228..>13404)
                     /locus_tag="J8C37_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305192.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DADOOS010000072        13398 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.072, whole genome shotgun sequence.
ACCESSION   DADOOS010000072 DADOOS010000000
VERSION     DADOOS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.072"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(14..706)
                     /locus_tag="J8C37_001756"
     CDS             complement(14..706)
                     /locus_tag="J8C37_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ5305193.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="J8C37_001757"
                     /pseudo
     CDS             791..967
                     /locus_tag="J8C37_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="J8C37_001758"
     CDS             complement(1143..1712)
                     /locus_tag="J8C37_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ5305194.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="J8C37_001759"
     CDS             complement(1786..2109)
                     /locus_tag="J8C37_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305195.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="J8C37_001760"
     CDS             complement(2263..3075)
                     /locus_tag="J8C37_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305196.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="J8C37_001761"
     CDS             3429..4277
                     /locus_tag="J8C37_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAZ5305197.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="J8C37_001762"
     CDS             4422..5363
                     /locus_tag="J8C37_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAZ5305198.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="J8C37_001763"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="J8C37_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="J8C37_001764"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="J8C37_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAZ5305199.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="J8C37_001765"
     CDS             complement(9827..12013)
                     /locus_tag="J8C37_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAZ5305200.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="J8C37_001766"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="J8C37_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAZ5305201.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="J8C37_001767"
     CDS             complement(12235..12675)
                     /locus_tag="J8C37_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAZ5305202.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="J8C37_001768"
     CDS             complement(12749..13288)
                     /locus_tag="J8C37_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5305203.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DADOOS010000073        13213 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.073, whole genome shotgun sequence.
ACCESSION   DADOOS010000073 DADOOS010000000
VERSION     DADOOS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.073"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            149..1018
                     /locus_tag="J8C37_001769"
     CDS             149..1018
                     /locus_tag="J8C37_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAZ5305204.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1227..2252
                     /locus_tag="J8C37_001770"
     CDS             1227..2252
                     /locus_tag="J8C37_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAZ5305205.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2291..3241
                     /gene="manA"
                     /locus_tag="J8C37_001771"
     CDS             2291..3241
                     /gene="manA"
                     /locus_tag="J8C37_001771"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAZ5305206.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3335..5038
                     /locus_tag="J8C37_001772"
     CDS             3335..5038
                     /locus_tag="J8C37_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5305207.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5040..7073
                     /locus_tag="J8C37_001773"
     CDS             5040..7073
                     /locus_tag="J8C37_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAZ5305208.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7084..7524
                     /locus_tag="J8C37_001774"
     CDS             7084..7524
                     /locus_tag="J8C37_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5305209.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7724..8686)
                     /locus_tag="J8C37_001775"
     CDS             complement(7724..8686)
                     /locus_tag="J8C37_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5305210.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8853..9530)
                     /locus_tag="J8C37_001776"
     CDS             complement(8853..9530)
                     /locus_tag="J8C37_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305211.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9794..11560
                     /locus_tag="J8C37_001777"
     CDS             9794..11560
                     /locus_tag="J8C37_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAZ5305212.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11575..12012
                     /locus_tag="J8C37_001778"
     CDS             11575..12012
                     /locus_tag="J8C37_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5305213.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12025..13176
                     /locus_tag="J8C37_001779"
     CDS             12025..13176
                     /locus_tag="J8C37_001779"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAZ5305214.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4487 a         2293 c         2731 g         3702 t
ORIGIN      
        1 tttttataag agtttttact cattaattta caaaaaacaa gcaaaatgcg aattttctca
       61 catttttttt aacaaaatca aagttattgc ttgaaagaag aagaatccat cgttaaaata
      121 aagcttgacg tagaggggag caagaaatat gaaagatttt caattggggg ctgccaccta
      181 tttaaaaagt gtagccattc gggtgaaaga ccgagataaa atgattgatt tttacaagaa
      241 cgttgttggc tttgatttga aaagagaaga aaacgaatta gccattttag gaacagaaga
      301 agctggaagt gaaatgttgc tgctggaaga aagtcctaga gcgaatgatc acatgggggt
      361 gatcaaaaag ctgaaccgtt tttcattgat tatccctagc acagaagaaa tggcagatat
      421 cctttgtcgt atccgtaaaa tgaattatcc aatcaaagaa gctttagaag atcatggacg
      481 tatggggatt ttgctcgtag acccagaaga gaatgaattg gaaatttacc atgaagagaa
      541 taagaacgac caaagagaaa ttccagaaac aatggatcaa gaagcattat taagaaaatc
      601 tgaagaaaaa ttcagtaaac tatcaacagg ttcttatttt gaaaaaatcc atttaaatgt
      661 tactaactta acaaaagaac gccaatttct taatcatgta cttggtttga aggtacataa
      721 tgaagaagag ggcatccatg tattgaatga aggagatttc catgttggtt tgacagaagc
      781 tagcggaggc gcaattgatt tgcctacaga tgaagtgatt ggacttgatt ttctgaaatt
      841 catgatcgat actgaaagca tggaaggact gattgctcac ttgcagcaac tatcagttga
      901 tttcttctta gacaaaaagc acacagtact cacaatctat gatgctatag gaatcgaatg
      961 gtggttcgtc agtgagccaa aagaaaagaa taaaactgac attcgatgcg acgcttaact
     1021 ttttctttct taacgattgt cgcatgtctc gtcattttat gaacggtcat actatcgctt
     1081 attctagaga tagaaagaga aaatagtata ccgggggaaa ctgcgcggtt tttcgcgcag
     1141 tttttttgtg taaaaaaggt tgattcctca tagattaagc tttaagcttg gactgtaaga
     1201 atctgataga aaagcaggag aaaaagatgt atccacacac gaaacagctt attttgaaaa
     1261 agatggaaga aggattttat ccaggtgtag cagtcgcctt cataaaagaa aatcaagtag
     1321 aaacatttac agccggcttt gcccaagtcg ttcctgaaaa gcagctgatg acagaagatc
     1381 tgttgtttga tgcagcctca ctgacgaaag tcgttgcaac cacttcatta gttttacgtc
     1441 tattagaaga tggaaagata gaactcgatc aaccgcttca taaatatctt cctgaatttg
     1501 aaaatcagac aatcactttg cgtcatttac tgacacatac ttctgatatc aaaacatgga
     1561 taccaaacag agataagcta tctcaggaag aattgatcgc cgcttatttg aagctagaac
     1621 caggtgagca actaggaaaa gaagtaaaat atacagatgc cggtacgatt cttcttgggt
     1681 tgatgctaga ggaaatttac caagaaactg cagtggaaat ctttcaagaa caagtattgg
     1741 aaccattagg aatgatgaac agcttatttc ttccttttcc ttccaaaaaa atcgttccca
     1801 cagaacaact gccttcgggt gaggttttgc gtggacaaac tcatgatccg aaagcaagag
     1861 ttttggctga acatgcaggt aacgcgggtc tctttattga tttgaaagac agcattactt
     1921 ttgtccaaat gtatttgaac aagggaaaaa tccgtaatgg tcgtttttta gaagaagaga
     1981 cgattgattc tttactgcaa gatcaaacac cgaaaaaaaa tggctatcgt tctttaggat
     2041 gggacttaaa aaaggatgta gaagacggtc atgcgattct ttttcatact ggttatacag
     2101 gtacgttttt cttgattgat ataaaaacac aagaagcgtt gatatttttg tcaaatcgcg
     2161 tacatcctgt cgatcatcgt gcggagtatc tcatatgtcg agatgaaatt gttgaaaact
     2221 ttttgaaaga gaaagcggta ttaccgaaat aatgctataa tagctatgaa aaatgagagg
     2281 agtttgaatt atgcaggaac ctatgttttt aaaaccagtt tttcaagaaa aaatttgggg
     2341 aggaagtcgt ttacgttcag tctttggttt tgatatccct aacgataaaa tcggagaaga
     2401 ttgggcgatc agtgcgcatc ctcatggtgt cagtgtagta gaaaatggcg aattcaaagg
     2461 gaaaagatta gatgaactat ggtcagaaca caaggaattg tttggccatc cttcagaacc
     2521 agtgtttcca ttattgatca agatcttaga cgcagaagat gatctatctg tacaagtaca
     2581 tccagatgat gcctatggga tgaaacatga aggtgaatta ggaaaaactg aatgctggta
     2641 tatcatcgat gctgaacctg gtgcagaaat catctatggt catcatgcaa agacaagaga
     2701 agaactagct gagatgatca aagatgggcg ctgggatgac cttttgaaga aagtaccagt
     2761 taaaaaaggt gatttctttt atgtaccaag cggaacgatc catgctattg gtaaaggaat
     2821 catgatttta gaaacacagc aaagttctga cacgacttac cgcgtgtatg actacgacag
     2881 aaaagatgcg caaggacaaa caagagagct gcatatccaa caatcgatcg atgtaacaac
     2941 tgtccctgcg aaaacaccag aattacaaat caaagaagtc cgaaaaggga attcatcgat
     3001 cgtcacttat ttagaaactg agtttttcaa tgtatatgag tgggatatta aaggaatcac
     3061 atctttcaaa aaacaagccc cttatacatt gatgacggta atcgaaggag caggagacct
     3121 tgtcgttgac ggaaaaacat atccattaga aaaaggaaca agctgtatta ttccaaatgg
     3181 cgtttctgag tgggcggtcc aaggtgaact agcaatcatt gcgactgaac caggcaaata
     3241 gttcccagat agctgaaagc agcagacggt ttttcgtttg ctgctttttc tacataaaat
     3301 ccgtccatca ataaatagaa gtatggtgaa aaagatggaa tacaaatgga aagcaaacaa
     3361 cagcagtcgc gtgtctatga aacaactgag aaaagctgga catcacttaa atgaaatgat
     3421 catgccaaat ctaagtctgt ttatcgcttg gggaatcttg tcattagcaa caaaaggtgt
     3481 gtctggcgat ttgcacacca cattagaaga ggtatgccat tggatgatcc agctgctttt
     3541 acccatcttg atcagttatt caggcgggaa atcggtaggg ggacaaaaag gggcagtcgc
     3601 cggtgcaatt gcttctttag ggttgatcgc tcgttccaat gctccacaaa tcgtaggagc
     3661 gatgattatc gggccgcttg ccggcatcgt atatgaacaa tttgtttcta gattccacgt
     3721 gaaattcaag gctggatatg aaatgcttgt cagcaaccta ctagtaggac tgactggtag
     3781 cgttatttgt ataattggaa tcgtttttat tgaaccagtt ttaaatagtg ttcacttgtt
     3841 gctcgcctcc gttgtgtctt ggctagtcac aatgaatctg ttaccacttg tcagtctgat
     3901 cttagaacct ctaaaagtac tatttttcaa taatgcaatc aatcatggtg tattgacacc
     3961 tttaggaatc gaattctcac aaacagtcgg gtactcttcc ttatttttga tggaggcaaa
     4021 tcctggacct ggtttaggga tcttactttc tatcctttgt tttgcagaaa aacaggaaaa
     4081 agtaaatgcg agtggagcat tgatgatcca tgcgattggc gggattcacg aaatctattt
     4141 tccatttgtc ttgctgcgac cgtatctatt tttagctgta atggctggtg gggcaagcgg
     4201 cacgttgata tttcaatggt tagatacggg attagctgca cctgtatcgc ctgggtcact
     4261 actcgtgatt tttgccaatg cgccttatcc acaactagct ggggtaagta tagggatagc
     4321 aatcagtaca tttgtgacct tcatttgtgc agcgatacta ttgaaattac caaataaaga
     4381 aaaagagaac aaaacggtca aagaaagggg agaaatcttg gaaccatcta tcattcattc
     4441 cgtcattttt gcttgcgact caggtatggg atcaagtgct atgggagccg ccctgctgaa
     4501 aaagaaatta gcagcagaaa aaatcgatct ttctgtcgga tacagctcta tctatgaatt
     4561 gaataaccaa gtaaatcagc tggttatcgt gcaacatgaa ctagcgggtc ttgcaagaga
     4621 aaaagtacct caaagtcaaa tctatgtact ggatcacttt ttggaaacag atatcttgct
     4681 gtcacgaata aaagaagcga tccaatttcc aaagcgggaa gaaaaaaaag agatagcaga
     4741 tatcacagaa caaaaaaagt cgcctgctaa tattgtattt ttgtataaaa acaacagacg
     4801 cggctcacaa actatgggca tgacgatttt aagtcaatta gcaaaaaaag cgaaaaaaca
     4861 gtttgttatc tcaaaagaat caatagaaaa aatcaaaatc caagaagaaa cgatttatat
     4921 cgcacaaaga gaactagctg taactgaaca gctagaagaa aagatccctc ggctagttct
     4981 agttgaacat tgggtagcta cgaatgaata cgaaaaactc gtgaagggag aaatataaga
     5041 tgtatttatc gcccagagaa aaacaattat tagcagaatt tctcagtagc ccgtcacctg
     5101 tatctatcca aaaaatgatg aatttattga aggtcagtaa acggactatt taccgcgaat
     5161 tagataatct tgaagtctca ttgaaatcgg taaatgcttc tttgaaaaaa gcagctagag
     5221 gctcatttgt cattgaggca tcagaagaag tattagagca attaaaagaa aatacagatg
     5281 ggaaaagtaa tgacttatcc actgctaaac gacaacacgc gattttagcc gagttgttac
     5341 tgacgacaga gccgttgagt cttgctcatt tcatggaatc atatcgcatc agtaatacca
     5401 ctttttatgc agatatcaaa cagttggaag aaagtattcg ccaactgcct ttggaaatca
     5461 ttcggaacca gggatacgag atcgaaggac cggaaaaata ccgccggctt ctgacagcga
     5521 atgtattaga attagaaatc aatgaatatg aactttttca tagtatttct ttcgatgcat
     5581 cactgaatta tttttttcga ttcgttgatc cgcaacatct atctcttgca cgcaaagtaa
     5641 tcggggaaga attgatccaa aaaaagacca atctaagtga tagaaagctt gaacatttgg
     5701 ttttgatgct gacattgacg atggagcgtg tgactaaaaa tcatttactg acaaatgaga
     5761 catataatag tctggcaaac aaagaattac tacagacagc taaacgattg ttttcacgga
     5821 tcgcttctga gacaaaacaa ctttatccag taaatgagat cgtctttttt gcaggattat
     5881 taaatgactt cacgaactca tttgatcaga atttctttga agagacgttc gatacacagc
     5941 ttgcttattt agtaaaacaa ctgattcaag aagtaagtga agagacgaat gtccattttt
     6001 atgaagatga aactttgtac aaaatgctgt tgactcattt atctggtgtg ttttcaagag
     6061 ccgttttaca agaagagaca ctgaccaatc cgattttaga aaaaatcatg gttcaatacg
     6121 aggaagttgc gaatgcaatc cgtaaagcag tagagcggat ttttcaagaa aaaaaattat
     6181 ccgaagaaga gatcgcttat atggtccttc attttgcgaa ttcattggaa cgaagtccga
     6241 aagaaagcaa tatcagtatt gcaggatttt cgccaagtgg attagcttct actagcatgt
     6301 tggaactacg attgaaaagg tattttccct tcatccaaca aattcatttc tatcggattg
     6361 ctgatttaaa aaaaatcgat ttacaagaag aatatgatgt tgttatttct acatctattt
     6421 taccaggata cggtggaaaa tatattttag tctccccgct actgctagaa gatgaagtca
     6481 agcagctaaa agaagctttt tcgcatattg agaagaaaaa aaggcaaaaa attacgtata
     6541 acagcaataa cgagtggatg atagaagata cttatgagga aacaatgcat ttcatggatc
     6601 aaatcaatca attactcggt cgttttttta tccaagaact ggataatcct gagacgattt
     6661 ccgaaacgat tcgcagctta tttccttatt ttcctgaagg actagtaacg gatgaagagg
     6721 tggttcatcg ccggctgctt aagcgatatg aacaagcacc tatcggtatt cctcatacaa
     6781 atatggggtt attccataca tccagctcat tgatttctaa accgatcttt tgtattttca
     6841 atttgaaaca tccgttgacc attgaaggga tggatagaca gccagtcgat ttgtcccgta
     6901 tgttagttat gctggcgcct tcgccaatcc aagaaaaaga cgggattctt ttaggaaaga
     6961 tcagtggagc aatcatcatg aatgacttga acaccgagat cttccgctca ggaaataaag
     7021 aaatcgttta tcagttgtta gcgaaaatcc tgattgaaga aataaagaaa tgaaggtgaa
     7081 aagatgatac agctaccagc agaaaaaata ttgctcgatc aatcctatca aaattggaaa
     7141 gaagcgttga ccgagatcgg ccagtggatg acccaacaag ggggaatcaa accggcatat
     7201 atccaatcaa tgatcgaaag acaagaaaaa gcgagtgtct atatcgggaa tttcgtagcc
     7261 ttgccacatg ggactgacaa agatgaagaa gtagtagaag aaggaatcta tttagtccaa
     7321 gtgcctgatg gtgtggactt tggaacaaaa gaggaaccaa aaatcgcgac actcttattt
     7381 gtagtggctt taaaaaaaga acatcagtta gcggtgctcc aagatttagc ctttttctgt
     7441 tcagacatcg accaagtaat ggcgctcagt gatgcgaaaa ccattgaaga agtccaaatg
     7501 atttttgaac atggacatat ataaccaatg ttcacagtaa aaaaagctgc cttgcatcaa
     7561 gtaataactt caaccaagat aaaaataaga ggagctattt ttgttgggag gttcttatta
     7621 ctgcggatgc aagcttttga aaactattta ttctagaaaa ccgtttattt gaaatagggg
     7681 acggtgacaa ggtttttgtc accgccccta ttatttttgt gttttaatag ctctttttta
     7741 tatagatagt gcccatacct atgaataata aaacgatact ccatatacca agtgtcaaga
     7801 taccattcat gaaagtaacc tgttctgaag ctagtggcga gaaaatatct cctcctatga
     7861 tcacattttg aaaatcaaca taagaagttg gcaagtaatg actgattgat tctatccatc
     7921 caatcgaaga aaacaagcga tactctgcta agaaaagaca aagaagtgta cctaaaagat
     7981 tgactagaga atgttcaaat aaagcagata gaagaaaact gattgtactt aaaaagagca
     8041 cccacaacaa caggaacaga aagctctgga aaagatactc atacatcgga aggattcgta
     8101 tctcctgacc aatgatcgtt cctacaggat aacggaagtc tcccaaacca tttttgatta
     8161 ttactagtat agagacgatc aaaaatggca tcacgagact tagcatagct gcaccataat
     8221 aaatggtaaa acggatgcta tgtttcttta ccatactatc cggtaataga ttgactagag
     8281 agattacctg ctttcttttt tcataagaga tgatggaaga aagaaacaag gcggttatac
     8341 ataaaatcaa aaaactagaa atcattcctg aaaaaatatt ttcgtagtaa tttgctaaag
     8401 ggagatgcgc tggcaagtca aaaatagaat atctttggat ctgatgttct ttcatataac
     8461 ggagcaactc gacacgtttt tgttgttcaa tgattggaat tccttgtctt tgaccagaaa
     8521 tcagtcgatc cagtatatct ttttcataat gataagttgc ttcgaccatt tgctggacat
     8581 tccctgtgtt ttttgcatgt aagattgctt cgatcagttt gttgcttttc tctaaacttt
     8641 ccacaaattc tttctcattc tcatcttctt tcaacgtttc aatcaaacgt tgcatctctt
     8701 catagttatg attcagttca tcgaattctg tataagcaag tttatgatca aaaaaagtgg
     8761 gaatgcaaag agcacgacca acaatagagg aaataaaaag tttactttgt tttttagtaa
     8821 gagattgcgt tcaaatgtaa gtaaagacat agttacacct catataattt acgataaata
     8881 gctaaaatct cttccatatt tgaggattct gtgacgattt ctccattttt tagaaaacat
     8941 gctttagaac aaatcgattg gatattcgcg atagcgtggg aagaaagaag aatcgtcttc
     9001 ccctctttct gccattgtct gatgaggcta ttcaagatat caatacttcc tgggtctaat
     9061 ccgttcaacg gttcatcgaa taacatgaca gatgaatcac tgatcaagta agcagcaagc
     9121 aagacatgtt gtttcattcc tagagacatt tttccaatcg gcaaagtaag gtactcagtc
     9181 atcttcaatc gttggaaaac agtttggcat tctactttgc tctgccaata gtatttgatc
     9241 aaagtcaaat aatcaacagc cgatagatag tcggataact gattcgttga ttctagaaaa
     9301 aagaaagacc ggcgataatc tgtataagca gtaggagaga tgttgtgaag ggacaattga
     9361 tcataagttg ttggcagcag ttgggcaatg ccattcagca aagttgtttt gccaaatccg
     9421 ttcggggcaa tcaaagcagc tacttctcct gattctacta aaagattcaa ttgtcgaatc
     9481 acacgttttt taccacgttt catgttcatg tttttgatca gtaatgtcat agataggcct
     9541 ccaatataaa atttaataac ataatagaat ggaaaacatc taaattaaat gctaacaaaa
     9601 gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt
     9661 ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca aagtttcgtg
     9721 ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc aaaaggagag
     9781 agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa aagcgagggt
     9841 acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag cactgattgc
     9901 ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg aggctttggc
     9961 taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt atactggcgg
    10021 aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga tgggtgtgat
    10081 tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc ttggtggata
    10141 tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt ttgaaatgct
    10201 agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag gtttttctgc
    10261 gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag agatcatttt
    10321 atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa tcttattttt
    10381 gaataatgca atcaatcatg ggattttgac accattggga actgaacaag tagcagaggc
    10441 tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg gcgtattact
    10501 tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag ccatgatcat
    10561 tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga aaccgttatt
    10621 attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat tgatggatgc
    10681 tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga tgacaccgtt
    10741 caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt cgtttggtat
    10801 ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg aaaaatctgt
    10861 tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc ctcaaggagc
    10921 tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc
    10981 gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc aaacggtgac
    11041 caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta ctcaggtcga
    11101 actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt
    11161 tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc agaaaacatc
    11221 agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca tcaaacaaat
    11281 caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg ggatgaccgt
    11341 gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag tcaatatcca
    11401 agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag cggaaaaagt
    11461 acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca atccgcaaaa
    11521 atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa
    11581 agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga agaagcgatc
    11641 cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata tatcgatgcc
    11701 atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc gattcctcat
    11761 ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca
    11821 gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt gttcggtatt
    11881 gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat
    11941 atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta
    12001 gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt
    12061 cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt cgtagatgtg
    12121 aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga attagcagat
    12181 gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca
    12241 gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat tggcccgaat
    12301 atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga aaaggaagga
    12361 agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc agcatttcta
    12421 gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata tatcggcttt
    12481 ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc tttattcgtc
    12541 caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc
    12601 caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg caaactattt
    12661 agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg ttaccagacc
    12721 attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa
    12781 acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca tgcgtatatt
    12841 gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct
    12901 cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt gagagaacta
    12961 caagaacata acttgacttg cccacatttg acggcaacaa tggggattat ttttaattac
    13021 tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg
    13081 gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg gaatatcaag
    13141 caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa aaggggaatg
    13201 tgacgaaatt ttt
//
LOCUS       DADOOS010000074        13071 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.074, whole genome shotgun sequence.
ACCESSION   DADOOS010000074 DADOOS010000000
VERSION     DADOOS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.074"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="J8C37_001780"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="J8C37_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAZ5305215.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="J8C37_001781"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="J8C37_001781"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAZ5305216.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..3432
                     /locus_tag="J8C37_001782"
     CDS             3229..3432
                     /locus_tag="J8C37_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAZ5305217.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3533..4195)
                     /locus_tag="J8C37_001783"
     CDS             complement(3533..4195)
                     /locus_tag="J8C37_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305218.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4208..5710)
                     /locus_tag="J8C37_001784"
     CDS             complement(4208..5710)
                     /locus_tag="J8C37_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5305219.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6258..7937
                     /locus_tag="J8C37_001785"
     CDS             6258..7937
                     /locus_tag="J8C37_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAZ5305220.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8064..8543
                     /locus_tag="J8C37_001786"
     CDS             8064..8543
                     /locus_tag="J8C37_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAZ5305221.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8762..9442
                     /locus_tag="J8C37_001787"
     CDS             8762..9442
                     /locus_tag="J8C37_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAZ5305222.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9450..10781
                     /locus_tag="J8C37_001788"
     CDS             9450..10781
                     /locus_tag="J8C37_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAZ5305223.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10796..11758
                     /locus_tag="J8C37_001789"
     CDS             10796..11758
                     /locus_tag="J8C37_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAZ5305224.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11869..12207
                     /locus_tag="J8C37_001790"
     CDS             11869..12207
                     /locus_tag="J8C37_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAZ5305225.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12286..12993
                     /locus_tag="J8C37_001791"
     CDS             12286..12993
                     /locus_tag="J8C37_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAZ5305226.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4153 a         2314 c         2876 g         3728 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3421 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3481 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3541 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3601 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3661 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3721 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3781 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3841 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3901 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3961 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4021 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4081 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4141 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4201 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4261 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4321 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4381 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4441 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4501 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4561 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4621 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4681 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4741 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4801 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4861 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4921 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4981 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5041 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5101 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5161 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5221 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5281 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5341 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5401 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5461 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5521 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5581 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5641 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5701 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5761 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5821 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5881 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5941 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6001 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6061 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6121 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6181 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6241 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6301 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6361 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6421 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6481 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6541 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6601 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6661 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6721 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6781 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6841 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6901 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6961 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7021 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7081 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7141 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7201 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7261 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7321 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7381 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7441 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7501 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7561 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7621 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7681 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7741 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7801 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7861 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7921 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7981 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8041 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8101 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8161 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8221 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8281 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8341 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8401 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8461 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8521 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8581 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8641 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8701 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8761 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8821 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8881 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8941 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9001 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9061 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9121 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9181 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9241 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9301 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9361 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9421 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9481 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9541 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9601 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9661 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9721 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9781 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9841 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9901 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9961 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10021 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10081 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10141 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10201 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10261 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10321 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10381 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10441 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10501 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10561 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10621 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10681 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10741 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10801 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10861 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10921 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10981 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11041 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11101 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11161 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11221 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11281 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11341 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11401 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11461 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11521 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11581 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11641 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11701 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11761 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11821 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11881 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11941 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12001 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12061 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12121 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12181 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12241 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12301 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12361 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12421 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12481 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12541 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12601 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12661 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12721 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12781 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12841 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12901 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12961 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13021 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga a
//
LOCUS       DADOOS010000075        13004 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.075, whole genome shotgun sequence.
ACCESSION   DADOOS010000075 DADOOS010000000
VERSION     DADOOS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.075"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            174..1310
                     /locus_tag="J8C37_001792"
     CDS             174..1310
                     /locus_tag="J8C37_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAZ5305227.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1447..1791
                     /locus_tag="J8C37_001793"
     CDS             1447..1791
                     /locus_tag="J8C37_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAZ5305228.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2062..3348
                     /gene="efmA"
                     /locus_tag="J8C37_001794"
     CDS             2062..3348
                     /gene="efmA"
                     /locus_tag="J8C37_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAZ5305229.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3486..4085)
                     /locus_tag="J8C37_001795"
     CDS             complement(3486..4085)
                     /locus_tag="J8C37_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAZ5305230.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4489..5610
                     /gene="mnmA"
                     /locus_tag="J8C37_001796"
     CDS             4489..5610
                     /gene="mnmA"
                     /locus_tag="J8C37_001796"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAZ5305231.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5856..7277
                     /gene="arcD"
                     /locus_tag="J8C37_001797"
     CDS             5856..7277
                     /gene="arcD"
                     /locus_tag="J8C37_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAZ5305232.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7291..8409
                     /locus_tag="J8C37_001798"
     CDS             7291..8409
                     /locus_tag="J8C37_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAZ5305233.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8469..9761)
                     /locus_tag="J8C37_001799"
     CDS             complement(8469..9761)
                     /locus_tag="J8C37_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ5305234.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9911..10471
                     /locus_tag="J8C37_001800"
     CDS             9911..10471
                     /locus_tag="J8C37_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5305235.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10600..10977
                     /locus_tag="J8C37_001801"
     CDS             10600..10977
                     /locus_tag="J8C37_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAZ5305236.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10910..12982
                     /locus_tag="J8C37_001802"
     CDS             10910..12982
                     /locus_tag="J8C37_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ5305237.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4239 a         2327 c         2801 g         3637 t
ORIGIN      
        1 aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc
       61 tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt
      121 tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa
      181 ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga
      241 cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg
      301 cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc
      361 acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag
      421 cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg
      481 ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag
      541 atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct
      601 tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg
      661 gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa
      721 accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa
      781 tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat
      841 tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga
      901 tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca
      961 acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt
     1021 atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg
     1081 gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg
     1141 gttcagcttg tacagcagga aatatcgagc cttcacacgt attggaagca atgtatggag
     1201 aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag
     1261 acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt
     1321 cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa
     1381 aaacctagta aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg
     1441 aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg
     1501 catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc
     1561 aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta
     1621 aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga
     1681 ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat
     1741 tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat
     1801 ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga
     1861 cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt
     1921 gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa
     1981 aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg
     2041 aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag
     2101 agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc
     2161 caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt
     2221 gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg
     2281 gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg
     2341 cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg
     2401 tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg
     2461 ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa
     2521 tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc
     2581 aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt
     2641 gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat
     2701 gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat
     2761 ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta
     2821 gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga
     2881 atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct
     2941 attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac
     3001 gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca
     3061 tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt
     3121 cgtgtcttag ggaacttcaa ctctttgatg aacctgacag gtccaattgg attattattt
     3181 gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg
     3241 attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa
     3301 ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag
     3361 ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc
     3421 ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag
     3481 ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca
     3541 gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg
     3601 cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg
     3661 cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg
     3721 tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc
     3781 ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc
     3841 aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca
     3901 tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact
     3961 gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga
     4021 gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa
     4081 ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac
     4141 tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc
     4201 aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc
     4261 cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg
     4321 ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata
     4381 attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac
     4441 cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac
     4501 agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt
     4561 ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg
     4621 gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct
     4681 caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt
     4741 gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac
     4801 aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta
     4861 gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt
     4921 ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt
     4981 tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa
     5041 cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa
     5101 aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact
     5161 cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt
     5221 caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa
     5281 gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc
     5341 acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc
     5401 aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg
     5461 gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa
     5521 gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat
     5581 caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg
     5641 ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa
     5701 tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg
     5761 aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag
     5821 tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta
     5881 gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga
     5941 tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg
     6001 ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag
     6061 atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca
     6121 tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc
     6181 tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta
     6241 ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag
     6301 gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt
     6361 taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg
     6421 tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag
     6481 gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca
     6541 accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat
     6601 tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag
     6661 caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag
     6721 cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga
     6781 tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg
     6841 gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta
     6901 atctattctt attgactttc ttagttactg attatgccta tcaatttgcc tattctttat
     6961 gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt
     7021 taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga
     7081 tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg
     7141 gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa
     7201 aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag
     7261 gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa
     7321 tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca
     7381 ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa
     7441 cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa
     7501 gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact
     7561 ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg
     7621 cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt
     7681 gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc
     7741 aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta
     7801 gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg
     7861 atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc
     7921 tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt
     7981 attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac
     8041 acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa
     8101 gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa
     8161 tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc
     8221 attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca
     8281 ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac
     8341 tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa
     8401 caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc
     8461 atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct
     8521 ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag
     8581 ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa tgcactggaa
     8641 acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa
     8701 ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca
     8761 gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca
     8821 ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt
     8881 attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg
     8941 gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc
     9001 agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg
     9061 atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc
     9121 tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac
     9181 agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag
     9241 ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa
     9301 ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca
     9361 agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact
     9421 aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa
     9481 gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag
     9541 ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt
     9601 gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta
     9661 aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt
     9721 tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga
     9781 aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct
     9841 cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg
     9901 aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga
     9961 actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga
    10021 tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct
    10081 aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag
    10141 tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca
    10201 agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg
    10261 aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga
    10321 agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg
    10381 ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat
    10441 gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa
    10501 gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac
    10561 taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg
    10621 ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa
    10681 gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc
    10741 catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac
    10801 ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg
    10861 ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact
    10921 aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca
    10981 aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt
    11041 attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac
    11101 gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt
    11161 aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga
    11221 tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa
    11281 accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa
    11341 gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa
    11401 agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa
    11461 tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa
    11521 aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca
    11581 agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact
    11641 tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac
    11701 ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta
    11761 tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat
    11821 cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa
    11881 agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg
    11941 agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact
    12001 tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta
    12061 tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac
    12121 aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca
    12181 agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat
    12241 gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc
    12301 agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc
    12361 agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca
    12421 agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga
    12481 tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg
    12541 aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag
    12601 caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa
    12661 tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa
    12721 ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa
    12781 aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac
    12841 agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg
    12901 cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact
    12961 aaaagcagca atgaagaagt aataagatat gaaagaggtt gtga
//
LOCUS       DADOOS010000076        12734 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.076, whole genome shotgun sequence.
ACCESSION   DADOOS010000076 DADOOS010000000
VERSION     DADOOS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.076"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="J8C37_001803"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="J8C37_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAZ5305238.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="J8C37_001804"
     CDS             complement(322..891)
                     /locus_tag="J8C37_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAZ5305239.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="J8C37_001805"
     CDS             1077..1979
                     /locus_tag="J8C37_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAZ5305240.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="J8C37_001806"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="J8C37_001806"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAZ5305241.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="J8C37_001807"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="J8C37_001807"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAZ5305242.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="J8C37_001808"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="J8C37_001808"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAZ5305243.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="J8C37_001809"
     CDS             8547..8732
                     /locus_tag="J8C37_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAZ5305244.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="J8C37_001810"
     CDS             complement(8794..9990)
                     /locus_tag="J8C37_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ5305245.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="J8C37_001811"
     CDS             complement(10160..11596)
                     /locus_tag="J8C37_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAZ5305246.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="J8C37_001812"
     CDS             11889..12698
                     /locus_tag="J8C37_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5305247.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DADOOS010000077        12635 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.077, whole genome shotgun sequence.
ACCESSION   DADOOS010000077 DADOOS010000000
VERSION     DADOOS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.077"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            105..1988
                     /locus_tag="J8C37_001813"
     CDS             105..1988
                     /locus_tag="J8C37_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAZ5305248.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2063..2347)
                     /locus_tag="J8C37_001814"
     CDS             complement(2063..2347)
                     /locus_tag="J8C37_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAZ5305249.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2337..3071)
                     /locus_tag="J8C37_001815"
     CDS             complement(2337..3071)
                     /locus_tag="J8C37_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAZ5305250.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3200..3823
                     /locus_tag="J8C37_001816"
     CDS             3200..3823
                     /locus_tag="J8C37_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAZ5305251.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4015..5136
                     /locus_tag="J8C37_001817"
     CDS             4015..5136
                     /locus_tag="J8C37_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAZ5305252.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5143..8271
                     /locus_tag="J8C37_001818"
     CDS             5143..8271
                     /locus_tag="J8C37_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAZ5305253.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8290..11484
                     /locus_tag="J8C37_001819"
     CDS             8290..11484
                     /locus_tag="J8C37_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAZ5305254.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11630..12337)
                     /locus_tag="J8C37_001820"
     CDS             complement(11630..12337)
                     /locus_tag="J8C37_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ5305255.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4309 a         2221 c         2656 g         3449 t
ORIGIN      
        1 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
       61 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
      121 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
      181 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
      241 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
      301 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
      361 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
      421 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
      481 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
      541 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
      601 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
      661 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
      721 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
      781 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
      841 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
      901 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
      961 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
     1021 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
     1081 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
     1141 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
     1201 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
     1261 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
     1321 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
     1381 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
     1441 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
     1501 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
     1561 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
     1621 aggaacaaga tttgttgaaa gacactgttc tgattggact aggaacgaat ggctcattct
     1681 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
     1741 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
     1801 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
     1861 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
     1921 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
     1981 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
     2041 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
     2101 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
     2161 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
     2221 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
     2281 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
     2341 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
     2401 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
     2461 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
     2521 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
     2581 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
     2641 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
     2701 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
     2761 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
     2821 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
     2881 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
     2941 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
     3001 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
     3061 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
     3121 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
     3181 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
     3241 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
     3301 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
     3361 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
     3421 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
     3481 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
     3541 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
     3601 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
     3661 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
     3721 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
     3781 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
     3841 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
     3901 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
     3961 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
     4021 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
     4081 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
     4141 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
     4201 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
     4261 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
     4321 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
     4381 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
     4441 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
     4501 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
     4561 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
     4621 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
     4681 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
     4741 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
     4801 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
     4861 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
     4921 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
     4981 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
     5041 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
     5101 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
     5161 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
     5221 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
     5281 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
     5341 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
     5401 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
     5461 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
     5521 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
     5581 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
     5641 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
     5701 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
     5761 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
     5821 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
     5881 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
     5941 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
     6001 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
     6061 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
     6121 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
     6181 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
     6241 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
     6301 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
     6361 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
     6421 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
     6481 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
     6541 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
     6601 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
     6661 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
     6721 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
     6781 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
     6841 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
     6901 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
     6961 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
     7021 cgaaaaagca agatggccaa tgaaagaata gagctggaag aaataagaca acaagcagaa
     7081 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
     7141 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
     7201 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
     7261 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
     7321 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
     7381 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
     7441 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
     7501 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
     7561 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
     7621 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
     7681 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
     7741 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
     7801 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
     7861 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
     7921 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
     7981 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
     8041 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
     8101 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
     8161 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
     8221 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
     8281 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
     8341 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
     8401 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
     8461 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
     8521 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
     8581 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
     8641 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
     8701 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
     8761 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
     8821 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
     8881 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
     8941 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
     9001 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
     9061 aatgaacaaa acgatcaagg aatacaattt tctgatcaaa acaaaccttt atcattcaaa
     9121 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
     9181 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
     9241 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
     9301 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
     9361 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
     9421 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggtgaa
     9481 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
     9541 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
     9601 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
     9661 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
     9721 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
     9781 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
     9841 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
     9901 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
     9961 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    10021 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    10081 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    10141 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    10201 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    10261 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    10321 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    10381 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    10441 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    10501 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    10561 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    10621 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    10681 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    10741 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    10801 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    10861 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    10921 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    10981 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    11041 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    11101 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    11161 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    11221 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    11281 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    11341 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    11401 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    11461 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    11521 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    11581 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    11641 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    11701 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    11761 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    11821 gaaattcttt ctcagccagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    11881 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    11941 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    12001 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    12061 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    12121 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    12181 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    12241 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    12301 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    12361 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    12421 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    12481 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    12541 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    12601 tttttttaat acagtctcaa tataactatt caaac
//
LOCUS       DADOOS010000078        12291 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.078, whole genome shotgun sequence.
ACCESSION   DADOOS010000078 DADOOS010000000
VERSION     DADOOS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.078"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(39..2114)
                     /locus_tag="J8C37_001821"
     CDS             complement(39..2114)
                     /locus_tag="J8C37_001821"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAZ5305256.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2116..3033)
                     /gene="glyQ"
                     /locus_tag="J8C37_001822"
     CDS             complement(2116..3033)
                     /gene="glyQ"
                     /locus_tag="J8C37_001822"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAZ5305257.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3417..4229)
                     /gene="recO"
                     /locus_tag="J8C37_001823"
     CDS             complement(3417..4229)
                     /gene="recO"
                     /locus_tag="J8C37_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAZ5305258.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4372..5271)
                     /locus_tag="J8C37_001824"
     CDS             complement(4372..5271)
                     /locus_tag="J8C37_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAZ5305259.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5286..5687)
                     /locus_tag="J8C37_001825"
     CDS             complement(5286..5687)
                     /locus_tag="J8C37_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAZ5305260.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5665..6141)
                     /gene="ybeY"
                     /locus_tag="J8C37_001826"
     CDS             complement(5665..6141)
                     /gene="ybeY"
                     /locus_tag="J8C37_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAZ5305261.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6158..8350)
                     /locus_tag="J8C37_001827"
     CDS             complement(6158..8350)
                     /locus_tag="J8C37_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAZ5305262.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8374..9345)
                     /locus_tag="J8C37_001828"
     CDS             complement(8374..9345)
                     /locus_tag="J8C37_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAZ5305263.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10051..11430
                     /gene="brnQ"
                     /locus_tag="J8C37_001829"
     CDS             10051..11430
                     /gene="brnQ"
                     /locus_tag="J8C37_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAZ5305264.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11584..12030)
                     /locus_tag="J8C37_001830"
     CDS             complement(11584..12030)
                     /locus_tag="J8C37_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAZ5305265.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12057..12233)
                     /locus_tag="J8C37_001831"
     CDS             complement(12057..12233)
                     /locus_tag="J8C37_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAZ5305266.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
BASE COUNT         3297 a         2607 c         2229 g         4158 t
ORIGIN      
        1 ttttgtgcct attctctggt ttatttacat attagaaatt attttgtgat gatttgatct
       61 aaacttgcta gcgacaaagc catggcagca atttgactta attgcgttaa tcggttctta
      121 cgtactgctg gttcttctgc catgaccatc gtctgatcaa aataattttc gatcagtggg
      181 cgaaggctta caagctgatc ataattttcg ttgatcgttt ggtcagcaaa gctttttttc
      241 attgcctcta ctgctttgtg cagttctttc tccgcttcat tttcgaacag ttcagggtct
      301 acttctgatt gtttttcaag ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct
      361 tccatcgatg gtttgaagtc ttcatctgca agatgctgct tgaacaattg tgctgctgca
      421 aatacttttg tcagatcttt ttgttctgca ttcaaaacag cttcgatgac atcatgacga
      481 atattcttcg ttgtcaataa ttggcgcaga cggcctttga taaagtcgat cacctgttgt
      541 tgcccttctg ataaaccaat gccgaaacga tccgtatctt tactgataac atcactaatc
      601 gtttcttgca gaacagacaa aggaaaggcc cattttttcg attcgatgat ccgaacgaca
      661 ccgtatgctt ggcgacgtaa ggcataagga tcgtttgacc cgcttggaat catcccaact
      721 gcaaagaaag aaaaaatgct atctaattta tctgcaatcg ctaaaacaga acctactgta
      781 gactgaggta gctctccttc actggatata ggcatgtaat gctcacgaat cgctgttgct
      841 acagcaggtt tctcgccttt cagcagagca tatttttcgc ccatgatccc ttgtagttca
      901 ggaaattccc ctaccatgtt ggttactaga tcaaatttat agatttcagc agctcgttgc
      961 agatccatca attcttcctc agatagtcct acttgttcgc caatgatacg ggcaatgact
     1021 tgtacccgct gcattttctc atagatacta ccgatttttt catggaaagt aacagttttt
     1081 agtttttcaa cgtattggtc gattgaagct ttcttgtctt cattataaaa gaattccgca
     1141 tcttctaaac gagccgtcaa tactttttga ttgcctcgaa ccacattttc caattttacc
     1201 cgatcaccat tacgagcaga aataaaatga ggaagcagca taccttgatc gttccgtacc
     1261 tcgaaataac gttgatgttc tttcatagaa gtgactagta cttcttcagg aacagaaaga
     1321 tatttttctt cgaagtcgcc agaaaagaca gttggatatt cgactaaatt gttgacttct
     1381 tctagtaaat cttcgtctag atcaataacc cagtgatttt tttcggctaa ttcatttgct
     1441 tgtgtaacga tcatttcttg tcttttttgc ggtgaagcaa taacgaattg ttcattcaat
     1501 ttttcttcat attcttttgc atgcgcaaat gtcacatctt cgcctaagaa acgatgtcct
     1561 cgagaggctc tgcctgtttt gacatctaag atctcaaacg ggattacttc gtcatctaac
     1621 aatgccacga tccagtgaat agggcggata tattcaaaat cgtattctgc ccaatgcatc
     1681 gttactggga aagtgaggct tttaatgaca tctgctaaac ctgtcaatac ttctttcgct
     1741 tctttgccgt gagtgtattt cgttacatag acatattcca cgccttttaa ttctctaaaa
     1801 gtgatagcat ctgtcgtcag accttgtccg cggacaaatc cttcagctgc tttcgtccag
     1861 tttccatctg cgtccaaagc aatttttttg gctggtcctt tgacttcttc ttcactgtct
     1921 gcttgacgtt ctggaatgtt tgttactttg attgctaagc ggcgcggtgt agaaaacgtt
     1981 tcgatcgtat cataagtcaa ttggttttca tctaaaaatt ttgctgtttt ttcttctaat
     2041 tgtttcatgc ttggtgtgac aacatgagca ggcatttctt ccagcccgat ttcaagtaat
     2101 aagtcttttg ccatcttatt cagcctcctt catgttttct ggatttgctg tgtttttatt
     2161 caataaaggg aatcctaatt tttcgcgttc tgccacaaag attttggcta cagcacgagc
     2221 catgttgcgg atacgggaaa gataacctgc tcgttctgtt acggatactg caccgcgagc
     2281 atctaataaa ttgaatgtat ggctgcattt caaaatataa tcatacgctg gatgaacaag
     2341 attttcatcg atacagcgtt tagcttcttt ttcaaaacgg tcaaagttgc ttagtagcaa
     2401 gtcttggtca cttacttcaa aagaatattt ggaatgttca tattctggtt gtttgaagat
     2461 ctcgccatat ttcacaccat cagcccattc cagatcatag acactttcaa cttcttgaat
     2521 atatgaagca agacgttcta gtccataagt gatctcagaa gtaactggtt tacaaggtag
     2581 tcctccgact tgctggaagt acgtgaattg tgtgatctcc atcccatcaa gccatacttc
     2641 ccaacccaca ccggcacacc ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat
     2701 atcgtgttct aatggatcaa ttcctaaaag acgcaagctt tctaaataaa gctcttggat
     2761 attttcaggt gaaggtttca ttacgacttg aaattgatga tgctgataca aacggtttgg
     2821 gttttctcca taacgcccgt cagctggtcg gcgggatggc tctacataag cagcattcca
     2881 tggttccggt ccgattgccc gtaagaatgt atatgggctc atcgtacctg ctcctttttc
     2941 tgtgtcataa gcttgcatca gcatgcagcc gtttgatgac caaaattttt gtaatgttaa
     3001 aatcatatct tggacggtca attttttact catctgtttt gcccctttca atttttaagc
     3061 gttgctgtca ataagaatac aggaaagaaa acgacaaaaa gtccctatgt ctgattacag
     3121 acatagggac gaataatttc gcggttccac cctacttccg aataaagatt cggcagcttc
     3181 atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg gtccggcttc cactctcccg
     3241 aactcgctaa accacgtgtt ttttactgat cttggtcaat gtacgtattc attttgcagt
     3301 tttcttgttt atataataga tcctctcagt gaacgatcta tttaatctac ggttaataat
     3361 agacggaagt ccctgtattt gtcaaatagt tttgattttt ttgttgtatc tttcatttat
     3421 tcttcttttt caggtatctc tttttcaggc atatttttct ctggcgtctt taatacgtgt
     3481 gcccaagact tcatttgatc aatgaacttt ttgcttttta gatgaatacc gacatattca
     3541 tcatacaact ggtcaatgag gtcacggatt gcctgcttcg tttcttcttt caagtggatt
     3601 ccactgatct tatcataaga aatagccgaa aacattcgaa cgaaataaac agcgcgagga
     3661 tctgcatgat aacggtgttc gtccattggc cagtgtttct cgcatatcac accgccagat
     3721 ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacacagtg cgtccaaatt
     3781 ggcgcgattc cgaagcgttg cagtatttga acttcaaaga tattcgtgat gatttctgca
     3841 tcatctcctt gatccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca
     3901 tattgatgat cttcgattgc tgcatctact agatttagca aataggtacc ataaccgctc
     3961 aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac
     4021 gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc
     4081 ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa
     4141 gattccgtaa agattttgac taatttatct ttttctttgt aattacggct agataaaatc
     4201 aagcctttcg attctgcaag ctggctcata ttctccctcc taaaaaataa atctggaaaa
     4261 gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc
     4321 ggatttttat ccagtttatt tctatactta acctataaga agttcagaat atcagtattc
     4381 gtcttttcgg tagccaaagt cttgcagata gacttttttg tctcgccagt ctttttggac
     4441 tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc
     4501 tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct
     4561 atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat
     4621 cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt
     4681 ctcacggatc aattcagaaa cgataaagta ttcaggatga tcagtcactt gatcatctgg
     4741 aaaatattgt gggccctctg gcatttcact gactaataca tccattaagc gttcgacatt
     4801 gtttccttct gtagcagaga tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc
     4861 ttcgatgatt cccagcaact cgtctggatg gatcttatcg attttattga taatcagata
     4921 aactggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccgcgttt
     4981 ttgatcagca ctgatcataa ataagactgc atccacttct ttcaacgcac tgtaagctgc
     5041 ttctaccata aaatctccta gacgatgctt tggtttatgg attcctggtg tatcgataaa
     5101 aatcagctga gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgtgc
     5161 cttgtcactc ataatggcaa ttttttgacc cacgattcgg ttcaataatg tggattttcc
     5221 tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct
     5281 cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaatgatg
     5341 acagcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca
     5401 atcggatgaa aatgaaaatc cgtaaacatg tccactacat tttcaaaaac agtattgatg
     5461 atctctacta tccaaacgag aaaaacagct aaaagcagcc ataaccactc tatccgatca
     5521 agctggaaaa tcacgcccat aaataaagca aggataccga acgcaacatg tttcctcata
     5581 ttgcgttctt cctcaaaaac ggttttgact ccttggacag caaattccac agacatcagg
     5641 aaatgtttgt ttttctctac gtttttatct tttgagtcca taggcatcca agatctcttt
     5701 ctgtaaacca aacatttctt tctcatcttc tggtgtcata tggtcatacc cgttgatatg
     5761 caagaatcca tgtaccgcta aaaagccaag ttcccgatca tatgaatggc cgtaagcttc
     5821 tgcttgttca gcagcacgtt caatagaaat catgatgtct cctagtacac gaggcaatgg
     5881 attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa
     5941 actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta cttggattgc
     6001 cgcattgtcc atgaaagtaa cagacatttc tgtttcctct ggcagcttta aataagatgc
     6061 tgcaaattgc agcaagtcat cgatttcttt cattttctct tctgagactt ggtttgtttc
     6121 atccataaag gtaatatcca ttatacatcg cttctttcta ttcttcttgt tcttccttta
     6181 tcttctcaac ttctgttttc attttaggat atttgatccg agaatggaag gtgctactta
     6241 aaccgctgat caatgatttt tccacttttc ggatttcttt caaggtcagt ccactatcgt
     6301 ctaattgacc atcggaaata cgctcttcga taagattatg gacaaaactt tcgatttttt
     6361 cgctcgtcgg atgatccatt gcccgtactg cagcctcaca actgtcagca atattgacga
     6421 tccctgcttc acgactttgc ggttttggtc caggataacg gaattgctct tctgttactt
     6481 ccggattgcg ttctttcgct ttgatgtaga aataacgcat tagcgtggtt ccatgatgct
     6541 gtttgcaaat atcgatcacc atttgcggca tattataatc ttccaatatt ttggctccat
     6601 ctgtcacatg tccaaagata atatgtttac tgtcttctgg caataagaaa ttgtgcgggt
     6661 tttccgctcc tgaaggcaag ttctcaacga aaaagctagc atgtttgatc ttgccgatat
     6721 catgataata gcaagctact cgagttagca aagaacgtcc gccgatttct gctacagcgt
     6781 tagcacttag attagctacc atcatactat gatgataagt acctggcgct tcttccagca
     6841 actgtttcaa taaaggatga tttggattgc tgagttcatt taatacaatt acactgtcat
     6901 cattgaccat caattcaata tatgggtgta gaccgatcgt cagcaagaat gagaagagtg
     6961 caccagtgaa cccgcagaca agcattaacc aagtcgttgc atcaccaaag ctcattcctt
     7021 gatagacgat caaaatgaca tccatcatca caggaaaaat aatgacccac atggcagcag
     7081 ataatccttg ttctgagatc cgtttgcgtc tgacaatcgt tgccatgagc ccagagaaaa
     7141 cataagacat caagataatc gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa
     7201 gtgcagatac tgcttggaaa ataccagcca tgatccctgc tcttcggttc aagaaaaagc
     7261 ttaaaacaag tggtgcaaaa gcagcgggat aaaacagcgg gatataagta gcttgttccg
     7321 tttggaataa ttggaaaaat ttcatcagca ctatgctgac tgacatagct gtcacataga
     7381 aaagcacgta ttttgttctt tctcctgcat tttggaattg aagtgaatta taaatcagta
     7441 cgccgatttg aagcaaaaca gctaaaacca tggcaattag cggaaaaatc gaagttgttt
     7501 gattagtcag tcctagtaat tccaattttt tcatagctga agcatcgatt tgattacctt
     7561 cacgaacaat gatttctcct tggaagatca tcactggttg tacagcttct cgtgcggatt
     7621 gtttcaattc ttctgtcttt ttctcattca atacgtcatt cactacgata ccttgatcca
     7681 ctaagtagcg aatcatttgt tgttgtgctg gtgtgacatt caaatattgg atttgctctt
     7741 ctgcttcttg tttaaaagct tccaattcac tttcacgaac ttgtttgctc atctgttcgt
     7801 caatgagctt cagactctca tccctcacag tatggatctc agtttcagtc atttcaaata
     7861 tcgtcgaata aaaattgttc ggtagttttt gataaaacgc aacattttct gcattcacat
     7921 tttcaaaatt tttcttcaac gcggcgatcc gctcttctgt agtaggcttt gggatatttt
     7981 catcttcctt tgccttatca atcttttcgt cataagattt gttgatggca tcattcgttt
     8041 tatcgatcaa ttcaaatagt tgcttgatcc gattgttctg gtcatctgca agatcttttt
     8101 ggtaagtgta ttctggagtg actgcttctg ctgccagttt tcttttttgt tcggtttctt
     8161 ccgtgttctc gatcgttttg ttcgcacgga tactttcttc tgctacttgg ccttctctga
     8221 aatcattggc cttttgtttg acactactaa atgtaacgcc gcagataatg ataaaaaaca
     8281 agaacagcag gaaaggcaat gctgcctttc caagtttttc ttgtagtttc tgcagtgttt
     8341 tattcaacat gtgcttatcc tcctaagcaa agcttattct tgatggagat ctgccttttc
     8401 ataagctttg atgatttcgg caactacagg gtgccgaaca acgtcacttg cttcaaaatt
     8461 aacaaaatcg attttcttga tttgctttaa tgttcgttct gcatgaacaa gaccacttgt
     8521 tgtccctcga ggcagatcaa tctgactcgt atctccattg acaatcattt ttgattgata
     8581 accaagacgt gtcaggaaca ttttcatctg agcaactgtc gtgttctgtg cttcatccaa
     8641 aataacgaat gcatcatcca atgtccgccc ccgcatgtaa gctaaaggag caatttcgat
     8701 cacgcctcgt tccatcaatc ggttcgtatg atctaatcca aaaatttggt ataaagcatc
     8761 ataaacagga cgcagataag gatctacttt ttcttttaga tccccgggaa gaaagcctag
     8821 attttcccct gcttccacag caggccgcgt caaaatgatt ttttgcactt cacctttttt
     8881 caaggcagcg attgctaata ctactgcaag aaaagttttt cctgtcccgg ctggtcctac
     8941 gccaaatacg atatcatgtt tggctaccga ttcgacatat ttcttttggc cgctgttttt
     9001 tacccgtatt ggttttccat tacggtcttt gacgatctct tcctcataca tttcaacaaa
     9061 ataatctaat gtgccttttt gccccatttt cagtgccgtc acgacatctg gtgtacttac
     9121 atggattcct cgtttgatca attcctgcaa tgtccgaatg actgaggcag ccaaggaaat
     9181 ctgggattgt tcgccaatga tctgaatgat ttctccacgt gtatggatgg ttgtttctgt
     9241 tgtttcttca atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc
     9301 agcagccgtc aatctaatct ctaaagaact agactcttcc gtcaaaaata gacatcccct
     9361 ttttttcgat tcattctttc ctattttacc atactcagaa gaaactatat agcaaataag
     9421 aaaaggttct aaaagagttg cttaaccata agtaccaaga aacgttaaaa aatagctgct
     9481 tccattctct aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga
     9541 tagaggacaa gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa
     9601 aaaaagaaaa caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa
     9661 cgtcaacaaa taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagcgattta
     9721 ttcccagtga gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc
     9781 gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac
     9841 tttatcgaaa agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt
     9901 ctagaatcta gagccctttt cccaagtgga aaagggcttt tttttatatt ttaaggtggt
     9961 ggttttcatg gaatctgata ttagtggcat ttctagtttc tgcaacgttt attttgttac
    10021 tcaaaatgat aattattgga ggaaaaattt atggaagaaa caaaaactta ttcatggaag
    10081 caattattga tggtcagttc acttatcttc gggatgttct tcgggtcagg taatctgatc
    10141 tttcctgtcc acttaggtca aatggctgga tcaaactgga tcagcgctgg tgtaggattt
    10201 gccatctcag gcgctctttt cccattatta gctatcttag ctgtagtagt gacaaaaagt
    10261 gatggtctgt atgacttagc taaacctgtc ggaaaatggt atgctgcctt gttcttgatc
    10321 cttgttcatt taacgatcgg accattcttt ggtacaccgc ggacagcagc aactgctttt
    10381 gaaatggctg ctaaaccctt tttgcctgaa aaatatgatt cattaggtat gtttttattc
    10441 tctgctcttt tctttttact tgcttatttc cttactgtac atccaacacg tttggtcaaa
    10501 tatgtcggaa agtatttaaa ctccttgttt cttgcattat tagctatcat ttttgtcgtt
    10561 tctttattcc aacccatggg gaatctaaac caatctgctg cagattctta tcagacgaac
    10621 gctttgacaa atggcgtttt agaaggatac aacacggttg atgcagtagc tttacttgct
    10681 ttaagtgtca cttttgtcca tgcagttcgt gatcttggtt ttaaagataa aaaagtcaca
    10741 gaattaacag caaaagctgg tagcctgagc gttgcgttag aagtcatgat ttattttggt
    10801 ctggtccttc tcggtgcgat gagtttgaat aagatgcctt tatcagaaaa tggcggaact
    10861 gcactttctc agatcactaa tttttattta ggtaacttcg gtgctagctt cttaggggta
    10921 atggtcacat taggtgtctt cactaccgct atgggactcg ttgtttcttt tgcacaagat
    10981 ttccataaac tgttccctaa agtcagttat atgacatggc tgcggttaac gacttttgtc
    11041 tcttttgtcg ttgcgaatgc cggattggat aatatcatcc aatggtcttt accagtattg
    11101 atgcttttgt atccattgtc acttgcgtta atcttgcttt ccttaacagc aaaattcttc
    11161 caaaagagtc catttgtcta tcaagtaacg atgttctttt ctgccgttcc agccgtattg
    11221 gatatgttgg caaattcgcc tgcattagtg agtcagcaaa ctgtcgtagc aagcttacta
    11281 ggattttatc atcatcacgt tccttttgct gcattaggac ttggctggat ggtcccaacg
    11341 ttacttggtt acgctggcag cttactcttt tattacgcct atcgcctatc agggtataaa
    11401 caagaagcaa atgaactacc agaagaataa ccaaatcaaa cacaatttaa cataagagat
    11461 attaaacagg aaaaaaacag ctagaagaaa gaatcttatt ctttcttcta gctgtttttt
    11521 agttcttttt ttacgaacac aaaaaactgc ggacaatcac tgtccgcagt ttaaatgata
    11581 aaattaagat tgtaatagtt ctttcacaat cgcattcact tggtttccat ctgcttttcc
    11641 tttgacttta ggcataattg cacccataac tttgccaaat tctttcattg atgaagcacc
    11701 agtttgagtg attgcttctt gaacaagttg tcgaatttct tcgtcggaaa gttgttccgg
    11761 catatatttt tcgactatta caatttcact ttttactttt tcagctaaat cgtcacgtcc
    11821 tgcttcttca aactcatgca aagaatcacg tcgctgtttc atttcgcgag acaacaccgt
    11881 tagttcttct tcttctgtca agtcgtgacc ttctttaatc tgctcgtttt gtatagatga
    11941 cttgatcatt cggatgactt gtaaagattc cttatctttc gctttcatcg ctgctttcat
    12001 gtcatcgttt aatctactta gtagtgacac actaccaact ccaattcatc acagaactag
    12061 aattttttgc gttttctagc tgcttcagat tttttcttgc gtcttacact tggtttttcg
    12121 tagaattcac gtttacgaga ttcttgtaaa gtacccgctt ttgacacgga acgtttgaag
    12181 cgacgaagag catcgtcaag tgattcgttt ttgcgaacga ctgtttttga catgttaatt
    12241 ccctccctcc gagcttaaaa agtaaccgta attgttattg aaatatatct c
//
LOCUS       DADOOS010000079        12218 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.079, whole genome shotgun sequence.
ACCESSION   DADOOS010000079 DADOOS010000000
VERSION     DADOOS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.079"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            68..751
                     /locus_tag="J8C37_001832"
     CDS             68..751
                     /locus_tag="J8C37_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAZ5305267.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="J8C37_001833"
     CDS             753..1589
                     /locus_tag="J8C37_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ5305268.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="J8C37_001834"
     CDS             1600..3354
                     /locus_tag="J8C37_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305269.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="J8C37_001835"
     CDS             complement(3630..3971)
                     /locus_tag="J8C37_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAZ5305270.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="J8C37_001836"
     CDS             complement(4039..6210)
                     /locus_tag="J8C37_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAZ5305271.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="J8C37_001837"
     CDS             6421..7278
                     /locus_tag="J8C37_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAZ5305272.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="J8C37_001838"
     CDS             complement(7349..8206)
                     /locus_tag="J8C37_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5305273.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="J8C37_001839"
     CDS             complement(8191..10893)
                     /locus_tag="J8C37_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAZ5305274.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="J8C37_001840"
     CDS             complement(10906..12195)
                     /locus_tag="J8C37_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAZ5305275.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         3374 a         2603 c         2220 g         4021 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttg
//
LOCUS       DADOOS010000080        12139 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.080, whole genome shotgun sequence.
ACCESSION   DADOOS010000080 DADOOS010000000
VERSION     DADOOS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.080"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            256..531
                     /locus_tag="J8C37_001841"
     CDS             256..531
                     /locus_tag="J8C37_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305276.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="J8C37_001842"
     CDS             544..2616
                     /locus_tag="J8C37_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAZ5305277.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="J8C37_001843"
     CDS             2781..4667
                     /locus_tag="J8C37_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAZ5305278.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="J8C37_001844"
     CDS             4679..5626
                     /locus_tag="J8C37_001844"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAZ5305279.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="J8C37_001845"
     CDS             5646..6173
                     /locus_tag="J8C37_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ5305280.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="J8C37_001846"
     CDS             complement(6236..6889)
                     /locus_tag="J8C37_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAZ5305281.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="J8C37_001847"
     CDS             7021..7863
                     /locus_tag="J8C37_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ5305282.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="J8C37_001848"
     CDS             8021..8917
                     /locus_tag="J8C37_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ5305283.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="J8C37_001849"
     CDS             8920..9633
                     /locus_tag="J8C37_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAZ5305284.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="J8C37_001850"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="J8C37_001850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ5305285.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="J8C37_001851"
     CDS             10168..10395
                     /locus_tag="J8C37_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAZ5305286.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="J8C37_001852"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="J8C37_001852"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ5305287.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="J8C37_001853"
     CDS             complement(11181..11723)
                     /locus_tag="J8C37_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAZ5305288.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..>12139)
                     /locus_tag="J8C37_001854"
     CDS             complement(11960..>12139)
                     /locus_tag="J8C37_001854"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAZ5305289.1"
                     /translation="ALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFSLEV
                     QDLDGNTLEPVWMKM"
BASE COUNT         4223 a         2068 c         2457 g         3391 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagc
//
LOCUS       DADOOS010000081        11825 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.081, whole genome shotgun sequence.
ACCESSION   DADOOS010000081 DADOOS010000000
VERSION     DADOOS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.081"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(37..618)
                     /locus_tag="J8C37_001855"
     CDS             complement(37..618)
                     /locus_tag="J8C37_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5305290.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(801..1427)
                     /locus_tag="J8C37_001856"
     CDS             complement(801..1427)
                     /locus_tag="J8C37_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ5305291.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1449..2780)
                     /locus_tag="J8C37_001857"
     CDS             complement(1449..2780)
                     /locus_tag="J8C37_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAZ5305292.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2935..3336)
                     /locus_tag="J8C37_001858"
     CDS             complement(2935..3336)
                     /locus_tag="J8C37_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAZ5305293.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3422..3628)
                     /locus_tag="J8C37_001859"
     CDS             complement(3422..3628)
                     /locus_tag="J8C37_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305294.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3723..5225)
                     /locus_tag="J8C37_001860"
     CDS             complement(3723..5225)
                     /locus_tag="J8C37_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAZ5305295.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5243..5698)
                     /gene="argR"
                     /locus_tag="J8C37_001861"
     CDS             complement(5243..5698)
                     /gene="argR"
                     /locus_tag="J8C37_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAZ5305296.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5796..6413)
                     /locus_tag="J8C37_001862"
     CDS             complement(5796..6413)
                     /locus_tag="J8C37_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305297.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6634..8007
                     /locus_tag="J8C37_001863"
     CDS             6634..8007
                     /locus_tag="J8C37_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAZ5305298.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8082..9194
                     /locus_tag="J8C37_001864"
     CDS             8082..9194
                     /locus_tag="J8C37_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAZ5305299.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9266..10903)
                     /locus_tag="J8C37_001865"
     CDS             complement(9266..10903)
                     /locus_tag="J8C37_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAZ5305300.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11071..11679)
                     /locus_tag="J8C37_001866"
     CDS             complement(11071..11679)
                     /locus_tag="J8C37_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5305301.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3122 a         2485 c         2136 g         4082 t
ORIGIN      
        1 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
       61 caatctttct ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
      121 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
      181 ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
      241 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
      301 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
      361 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
      421 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
      481 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
      541 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
      601 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
      661 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
      721 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
      781 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
      841 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
      901 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
      961 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
     1021 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
     1081 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
     1141 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
     1201 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
     1261 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
     1321 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
     1381 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
     1441 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
     1501 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
     1561 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
     1621 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
     1681 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
     1741 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
     1801 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
     1861 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
     1921 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
     1981 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
     2041 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
     2101 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
     2161 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
     2221 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
     2281 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
     2341 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
     2401 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
     2461 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
     2521 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
     2581 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
     2641 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
     2701 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
     2761 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
     2821 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
     2881 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
     2941 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
     3001 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa
     3061 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
     3121 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
     3181 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
     3241 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
     3301 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
     3361 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
     3421 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
     3481 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
     3541 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
     3601 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
     3661 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
     3721 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
     3781 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
     3841 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
     3901 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
     3961 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
     4021 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
     4081 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
     4141 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
     4201 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
     4261 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
     4321 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
     4381 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
     4441 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
     4501 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
     4561 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
     4621 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
     4681 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
     4741 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
     4801 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
     4861 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
     4921 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
     4981 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
     5041 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
     5101 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
     5161 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
     5221 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
     5281 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
     5341 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
     5401 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
     5461 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
     5521 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
     5581 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
     5641 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
     5701 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
     5761 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
     5821 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
     5881 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
     5941 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
     6001 atctttttga atactagttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
     6061 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
     6121 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
     6181 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
     6241 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
     6301 ttcgggtcgc cgatccctct gatgctaaag aatggttttt taggaacctc tagtatttgc
     6361 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
     6421 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
     6481 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
     6541 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
     6601 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
     6661 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
     6721 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
     6781 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
     6841 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
     6901 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
     6961 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
     7021 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
     7081 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
     7141 tttcttgtgc ccgttttgat tctttctttg attatcggct ggtttgctat tcccgagatg
     7201 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
     7261 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
     7321 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
     7381 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
     7441 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
     7501 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
     7561 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
     7621 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
     7681 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
     7741 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
     7801 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
     7861 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
     7921 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
     7981 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
     8041 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
     8101 tcggtggcgg gatcattggt atgactctag ccagttactt agatacatca aaattcaatg
     8161 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
     8221 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
     8281 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
     8341 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
     8401 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
     8461 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
     8521 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
     8581 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
     8641 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
     8701 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
     8761 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
     8821 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
     8881 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
     8941 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
     9001 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
     9061 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
     9121 taaatactgg tcattggtct acggaagatt atcaaaagga catctccacc tatattacga
     9181 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
     9241 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
     9301 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
     9361 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
     9421 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
     9481 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
     9541 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
     9601 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
     9661 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
     9721 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
     9781 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
     9841 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
     9901 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
     9961 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    10021 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    10081 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    10141 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    10201 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    10261 ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga
    10321 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    10381 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    10441 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    10501 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    10561 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    10621 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    10681 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    10741 tcgttcctgg actccctttt gctagttgat aagctgtctt cctgtcaaaa acttcatgaa
    10801 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    10861 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    10921 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    10981 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    11041 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    11101 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    11161 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    11221 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    11281 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    11341 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    11401 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    11461 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    11521 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    11581 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    11641 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    11701 gttttttatt ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga
    11761 aacatgacat ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata
    11821 ttttt
//
LOCUS       DADOOS010000082        11758 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.082, whole genome shotgun sequence.
ACCESSION   DADOOS010000082 DADOOS010000000
VERSION     DADOOS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.082"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1796)
                     /gene="uidA"
                     /locus_tag="J8C37_001867"
     CDS             complement(<1..1796)
                     /gene="uidA"
                     /locus_tag="J8C37_001867"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAZ5305302.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNF"
     gene            complement(1824..3590)
                     /locus_tag="J8C37_001868"
     CDS             complement(1824..3590)
                     /locus_tag="J8C37_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAZ5305303.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(3603..4397)
                     /locus_tag="J8C37_001869"
     CDS             complement(3603..4397)
                     /locus_tag="J8C37_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ5305304.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(4408..5784)
                     /locus_tag="J8C37_001870"
     CDS             complement(4408..5784)
                     /locus_tag="J8C37_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5305305.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(5913..6560)
                     /locus_tag="J8C37_001871"
     CDS             complement(5913..6560)
                     /locus_tag="J8C37_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAZ5305306.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(6644..8071)
                     /gene="uxaC"
                     /locus_tag="J8C37_001872"
     CDS             complement(6644..8071)
                     /gene="uxaC"
                     /locus_tag="J8C37_001872"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAZ5305307.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(8145..9224)
                     /gene="uxuA"
                     /locus_tag="J8C37_001873"
     CDS             complement(8145..9224)
                     /gene="uxuA"
                     /locus_tag="J8C37_001873"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAZ5305308.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9227..10858)
                     /locus_tag="J8C37_001874"
     CDS             complement(9227..10858)
                     /locus_tag="J8C37_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAZ5305309.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11175..>11758
                     /locus_tag="J8C37_001875"
     CDS             11175..>11758
                     /locus_tag="J8C37_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305310.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEA"
BASE COUNT         3720 a         2356 c         1551 g         4131 t
ORIGIN      
        1 ctgaaatttg gtattgatga ccatcgtttc tttagatagt gagcaatcat tttaggcttt
       61 ctatctctag taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt
      121 tcaaaatctg caaaattcca cacttgttca ccaacaaaat tgtcacattt atctattact
      181 tcgtgatttg cttcgtaata acccacttga tactcttctg taaacattgt tggtactgta
      241 tcatgaagtc cagctactgt atctgctcca tattctgtaa acattattgg cgtatcggga
      301 catctttcca tccatccatc tagctctttt ttcaacagat attttgcatg ttttaggtct
      361 ccacctgaaa cataccaacc ataatagcga tttaaacata aaacatcgac aatatctcct
      421 actttacaaa tatctggtgt tgactttaag taagtaacga ttgttacagg tctcttctga
      481 gggtcaagtt ctttagtcaa ttcaactaaa ggttcaaaat attctttagc gccttcagaa
      541 tccgaatctg gttcatttgc aatagaccac attacgacac tcggatggtt cttgtcgcgt
      601 tctatcaatt ccttaatcgc cattttgtga gcattttctg tccgtatttc tttccaagta
      661 tcatgttttt ctacgttgtc atctaataaa gtagccatga aattcaaatg taatcctact
      721 gcaggtactt catcaataac tacaatacct tctcgatctg ctaagcgcat aatttcttct
      781 gaataaggat aatgtgaagt cctaaatgaa ttagcaccga tccatttcat taaattaaaa
      841 tctttgatat ttactgcttc attcaaacca cgaccattaa tatacgtgtc ctcatgcttc
      901 ccaaatccct taaagtaaaa aggtttatta ttaatcaaaa acttcccatt cgaaacctga
      961 atactacgaa tgccaaattc ttctgtgtac gtatcaatca ctttattttc gttaattaaa
     1021 ctaactttca tactatataa gtaagcattt agaggctccc aaagtgttgg gttcttaagt
     1081 tgaatcactc cttctttccc cttagcttct gctataagtt gagcttcttc gtcatagatt
     1141 gctattagaa tttctccggt ccattctgta tcgatattaa attctacatt tgcatctgaa
     1201 tcaattattt ttgtattaat agtgacatcc tttatatagt ctttcggagt tgtatacaat
     1261 ttaacgggtc tattaatgcc tgcataattg aaaaagtcaa aattaaccaa attttctttt
     1321 tttatgtctc catcttcctg tatcgtttct ttcaaaatcc ctacaggtaa agtgctatcg
     1381 tcaataacat tgtttacagc cactgtaatt ctgttacttc catcttttat aaactgatta
     1441 atttctgatt caaatggcgt aaaccctcct ttgtgttgat taatcaattt tccattcaaa
     1501 aatacttttg cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat
     1561 tttttaggaa gaatcacttc tttttcatac cacacccatc caacatgatt tctaatgttc
     1621 tctgtttcaa ataaatcatt ataagaagaa ggaactgaaa ttggataact ctctttttca
     1681 accattggtt ctaaagcaag ttcttcttgt aagtcatcat ttgtatctaa cttaaatctc
     1741 caaagaccgc ttaaatcaat tacttgcctc gtctctgtca taatgggata taacatgtaa
     1801 atcactccta tataatttaa atattaaata aatgtatcct ttaacccaca ataagcatct
     1861 attggactta ttcccttaaa ttcagaaagg cccattagtt tgtccactaa tatttctaaa
     1921 gtttcttgtt ttccatcata agtattgata taagttttta cttgaggcac atcagctaaa
     1981 tgaaacggac actgtacaga tacaaaaatt gttggaattt catgaatata aaatggaata
     2041 tccggagtac ctttacttgc tggccaaaca attctttgaa ctgtacctgg gttaacatta
     2101 gctatattaa taatcaagtc atatttttca gttaaatctt tgattggtct cttagcagca
     2161 tatattccag ctatattttt aattttttct tcttgcgata atcctttcaa ttcttcttca
     2221 ggtgaaatgt atttttcaac ttcaaatcct tttgctgaca aaatctgttt caataaatca
     2281 atggcctgag gtctatttcc agcaactact tcaccaaacc caccttttac cccatgtaca
     2341 tctaccagta aaatcctttt atatttttct ggtacaatag gccaaatatc ttgtttatct
     2401 ttaactagtg taattgcttg atcagctacc tctttaaaaa ttgatttatt ttcaattaaa
     2461 ccaatttttt ccatagcttc ttgtttcggt aacaaaattt tattcttttc ttttttatgc
     2521 aaacctagtt tagcctttaa gcctaaaatt cttcttaatg catcacgtaa acgctcttct
     2581 gaaataattc ctttgtaata accttccatc atataattaa agtcttcatc tggatcatta
     2641 aaaaatagga atagatcaca tccagccgca atagaagtgg gcaataattc tgaccgtttc
     2701 attgccccag tcattcctaa catatgacta gcatcagtaa ctactaatcc attaaacccg
     2761 agttttcctc taagtaagtc agtgattaat tcttttgata aagttgcagg aagtaaatct
     2821 tcatctttca gatcaagatt aaaatgtttt gaataagctg gcatatggat atgacctgcc
     2881 ataattgaag gtaatccagc ttctattaag cctgagtata cttttccaaa agaattatcc
     2941 cattcttctg tacttaaact attaatacta aaagatagat gttgatctcg ttcgtcaata
     3001 ccgtcaccag ggaaatgttt agctgcaggc aaaattccac tctcttgaat acctttcata
     3061 taagctaaac taagttctaa aactttatca gggctttgtc caaaagatcg actagaaatg
     3121 ataggatttc tccaatttct attaatatct acaataggag caaaactcca gttacatcca
     3181 atagccgatg cttcaattcc agaaactctc cctaattcat aagcatattt cgcatcattt
     3241 gttgcaccaa tttttacttc tacaccaact tcggtaccat ctgtacaagc tccgttacca
     3301 cctgcttctg tattagcagc aattaataaa ggaattttgc tttctgtttg taggatatag
     3361 ttttgatcat atacctcatc agcagttcct ggattatatc gtacagctcc aatatggtac
     3421 tgattgacca tactacttaa gtaattttta tcacgactag atcccatatt aacaaataat
     3481 tgacctattt tttcttctaa agtcatagca tcaatcgtct cttcaatcca agaaatagca
     3541 tcttcatcta aatgatatgg tttttttcgt aagtctactt ttatagtcat tttctttctc
     3601 ctctacttat tagtaagatt tttccaaaaa gtatctatat atttttgttc aatatctata
     3661 tactctcccg ataaacattt aaataaaata tcagttctta agttttccca tgattctttt
     3721 tctttattaa ccaaaatagg ataaaagaaa actttattct tatttgcagc ttctagatca
     3781 ccaggagaat caccaatcat taatatctta tccaaatcac aaccattttc aattacactt
     3841 cgaataatgt cttctttttt tcctttatct tgacaacaca gttcatcaac taaggataat
     3901 aattcatgtc tttcccattc ttcttgaata gcttccttat tagctgagct tacaataatt
     3961 atcttcccat attttttcag agcttttata gtatctctag cacttaaaaa gggtttatct
     4021 aatcctttag cttgactaat ctgtcgattg acttcttccg accattttaa agcttttaat
     4081 aaatcctctg actgagagta ttctatttcc ctcttcaaag atggattaga caattctttt
     4141 gttacagata cccagtgctt taatcgatta atcgatggta catcattttt ttctaatgtt
     4201 ttaactaaac ctaaaaatct gtttattcca cgagtctctg aatataaatt aatattattc
     4261 caatcctgta ggaatttttc cctatgatga acatcaaaaa tctctgcagc aattggcccg
     4321 aaaaagagat tgtgtttata agtcattgta tccatgacac aaccatctga atcaatacaa
     4381 aatatccaac tgttcatgtt ttttctccta gttatgtttt tgatttagca aattataatt
     4441 ttctaatact ttttctttag ataatggata tacgaaaaat aaaatcaatc ctacaacaaa
     4501 gaaacaaact gctggaatca acgttgaaga gttatacagc ttttgtaaaa cttctccact
     4561 ttgtactttt gctaacgaat cataccctat tatagaaaga gtccaacctc cgacaccgcc
     4621 agctaaagct tgtccaattt ttcgggcaaa agaatatact gcataaatag ttccatcttc
     4681 tctttggcca gatttcactt cttgatcatc gattacatca gtaatattcg cccaaatgac
     4741 tgtattaaaa atacctaacc ccatatatcc aacagaagta agaactaaga atacatacat
     4801 attactaggc tttatcaaaa ataaaagtaa atatacaata gcagagaaga acatccctac
     4861 tgacgccaat tcttttttac cgtatttttt actgaaaatt aaacttacag gtgaaacaag
     4921 taccaacatc aaaataggat tgataaagtt cattaaaact attccctgtg cattattata
     4981 ataatctgga aatacataat tgttcatact cattgttaaa agctgagcaa gcaacaaaaa
     5041 aatagctgca gctattatag aaagcaacgc tctattttta gctaatcctt ttaaagattt
     5101 caacaatgaa acttttaact gcgattcatc acttttaacc cgttctacag ttaatttata
     5161 acatattacg taacaaataa ctgcacacac tgaaaatact cctgccacaa ttgtaaaagt
     5221 tgatccacca cgtacaattt gagctccaga actatctctt gtataaataa aaattggtgt
     5281 taacacgcca atgaacatac ctgctaaggt agctcctata ctacgaaatg ttgataacgc
     5341 tgtcctttct tctgcaacag aagtgatagc tgatgccatt gaaccgtaag gaatattaat
     5401 agatgtataa aaaatacttc cccatagtat atatgttaca tacatatata taactttaat
     5461 actcatagaa gctgatgcca ttgatgtttg atacattaaa aaacttgcta aagcgactgg
     5521 accactcatc cgtaaaatcc acggtttaaa tttgccagtt ttagatggtt ttgatccatc
     5581 cacaatacgc cccattgtta tgtctgtaac tgcatcaata aatcttgcta ctaaaaataa
     5641 tgttcctacg gtagctccgc ttatccccaa tacttttgta taaaaaatca ttaaaaaaga
     5701 actagcaaaa atgaaagtga aatcattccc aaaatctcca aacatatatc ctattttgtc
     5761 tcttagacca aattttcttt ccatataatt tgcgctcctt cctattgtta agcgttttca
     5821 ttctgttgaa ctgccaactt acgtactata ctctgaagta taaagagtta gtatttattt
     5881 tttacatttt atagtattat ttattttttc tcctaataat tctttatctc ataaaactta
     5941 tctatatatt tactagctat tcgagttact tcttcaaaat cacctttttc aacaggagcc
     6001 aacaaattcc cacctacacc aactgcaaca acgcctgctt caaaccactc ttctaagtta
     6061 tctagactta ctccgccagt aggcattata tttacatatg gtaatggagc ttgatacgtt
     6121 tttacaacag atggaccaaa agcagaacct ggaaatagtt taatgatttc tactccagcg
     6181 gtaagtgcct cctttatctc agtaattgtc atacaacctg gtaaataagg aatttgatat
     6241 aaattacaaa tctctgctgt ttgtcgatca aaagatggac ttacgatata ttctgctcca
     6301 gccataattg caatacgagc agtaaacgca tctaaaactg taccggctcc aattattaca
     6361 tctttttgat cagcatattt tttagcaatt tctttgatta tttcttcagc attcggaact
     6421 gtaaaagtta gttcaatccc tttaattcca cccgcagtaa ttgcatttat tgctttaatg
     6481 gcctcatctt tattttttcc acgtacaacc gcaatgactc cagccgcttc taacttactt
     6541 aaaatttcta cttttttcat atctatctcc ttcttttatg gatacatact ctagctagtt
     6601 gtactaactt acaataaata aatttaagct tttatttttt gtattatttt ctactttcga
     6661 tttcaaaaaa attaaaataa ttatacgcat tacgataaca aatattctcc actatctgac
     6721 taagatacat ttcatcatct ggaattcgac ctgctttaac ccactcgctt agtaattgac
     6781 ataatactcg acggaaatac tcatggcgag gatagcttag aaaacttcga gaatcagtca
     6841 acatacctgt aaaattcgac aacaaacttt gttgcgccat cgtagtcaac tgttgacgca
     6901 ttccttcagc tgtatcattg aaccaccaag cacatcccaa ttggattttt tgaatcatac
     6961 ccccttggaa gctttgcatt gctgttgcta gtggaagcca atcggttgga tttaatgaat
     7021 ataaaattgt ttttggtaag cacccactct tttcagcagt gtcgaacaat gctacaacat
     7081 tttgtgtcaa actagcttga tcccccatcg aatcaccacc gacatctact ccaatttgtt
     7141 cctttaatat acttgaatta tttcttaaag catttaaatg catttgcatc gtccaaccat
     7201 atcttgtata ctcagacatc aaatgtaatt gaatacctgt aacaaattgt gcaatttctg
     7261 ataaatggaa atccgttttt cctgctaaag cttttattaa cagattgtcg agtatttttt
     7321 catctgctat ttcataaaaa taagtattca aaccgtgatc tgctaatcga cagccaattt
     7381 gatgaaaata atccactcgt tggctcaatg cctgcttcaa cccattaaag tttttgattg
     7441 gtatacccgc tgcttgacta agttttaaaa tatagttcga aaatcctgat gatttgatac
     7501 tccagacctt atctggacgg aaagtaggta ataccttgaa agaattatcc tgtttagaaa
     7561 gctcagtatg atatcgcaag tcatctgtag gatcatccgt tgtacaaatg gctttcacat
     7621 ccatcatctt cagcaattct tttgggcgat aggaatccgt ttggattttt tcgttggcac
     7681 gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa atcaatatca aagtaacgtt
     7741 gtaattccaa atgagaccat tcaaaaatag gattacctgg cgccttctcc actgttttta
     7801 caaaagctaa aaatttttca taatcattac ctgcaccact gatcaactct tcttctatcc
     7861 cattagctcg catcaatcgc catttataat gatctccagc tcctttatca tttaaccata
     7921 cctgagtcaa attcttgtat tgcttatttt ctagcaaaca tgctgggtct aaatgacaat
     7981 gataatcgat gattggttgg tgttctgcaa atttatgaaa aagtctttta gctatttcat
     8041 tctctaataa aaagttctta tctaaaaaca tatttaagca ctcctttcct tcccttagtt
     8101 tttagaagag aaaatttttc ttctaaatag actccattat ataattattt actcgccccc
     8161 ttttcaagcg cttcccaaat accatttaaa taaactgcac ctagagctcg gtcatataac
     8221 ccataaccag gtctaccggt ttctccccaa atcatccgac catgatctgg acgaatatag
     8281 ccattaaatc cattatcgta cattgttttt aatatcttat acatatctag agaaccatat
     8341 tctgatttat gcggcgcttc ataaaaatcg agtccattat cacttaaata tttgatatta
     8401 cgaacatgtg caaaaggaat ccgatcccgt tgacaaaatt cagatagaat ttcatagaca
     8461 tcattatctg gtcgttctgc aatacttcca gtacataacg taataccatt agaaggagaa
     8521 tccactacat tacacaacca ttctaaatcg tcacgatttt taacaatacg aggtaatccg
     8581 aaaatagaat atggtggatc atctggatga actgccattt taatcccaac ttcttcacat
     8641 gtcgggataa ttgctttgag aaaatatgca aagttctctc gcaatttcgc ttcatcaact
     8701 tctttatagg cttctaataa ttgcttgatt tgtgataacc gatgaggttc ccaacccggc
     8761 aattcaaaat tattagtaga attttctact tttctaataa tttcctctgg atcagtagga
     8821 atattttttg aaatataggc taacgtagaa gacccatcat ctaaatgata atccaaatct
     8881 gattttaccc aatcaaagac tggcataaaa ttataacaga taacctcaat cccaaattct
     8941 gccaaattgc gaatcgtctg tttataattc tcgatgtatt gatctcgtgt tggcaaacca
     9001 attttgatat catcatgaac attaacactc tcaataactt tcaaggtaag gccattggct
     9061 tcgacttcat cttttaaagc tttaattcgt tctctcggcc agacctctcc ggctggtata
     9121 tccattaatg ttccaacaat tcctttcatt gctgggatct gacgaatttc ttgtagtgag
     9181 attttttctt catttgcccc ataccatcta aaagtcattt ccatagttac tttcctccaa
     9241 cttctgttat atatttattg attgttttac gaactgcttg aggacctact agcatttcgc
     9301 aaaagatttt ttcaattttc tttcctatct caacttgata taaatctaaa ccaaatattt
     9361 ccttattttc taagatttcc tgtaccgcac aatgaatctt tgcaggatct tgttcaccta
     9421 atttcaatac tgagaatttt tcctgcaact ctaccaataa aggatcagga cttggcacaa
     9481 aattttttcc ttgatcatcg actgccaata aataacgtaa ccaagcagca atagcgatag
     9541 gaataaatgt tagattttct gcatttccgt ctttttcgac atacttttta attgtttcac
     9601 catagcgaat cgctactttt tgagaagtat ctgttgcaat tcgttgcggt gtatctggaa
     9661 tatagggatt aggcaaacgt ttattgatta cttcatcaat aaattgtttt ggatcaataa
     9721 cgcctggact ttcaactact ggtaagcctt ctacataacc tatcccttta attaagccaa
     9781 ccaaatcatc atcttgcatt tctgcctgaa ttgatttata ccccaacaaa caacctgtta
     9841 ttgctaatgc cgtatggagc ggatttaaac atgtcgtaac cttcatgata tctgctttat
     9901 caactgtttc tctatctgtc ataatcacac cagccgcttc cagtttaggt cgaccatttg
     9961 gaaaactatc ttcaatgact agataatgga ctttttcagt attggtaaaa gcagcgatat
    10021 tggtattttt cgcagtatga attatatcta agtcttcgaa acccgccttc aataaatcca
    10081 ttttgacact ttccgaagga tttggcgtaa ttctatcaat catactccaa ggaaaagtca
    10141 ctttttcttt atcctttaaa taagcaataa atttttcaga aacgtatcct tttttttgcc
    10201 aagcttctgc aatcataaaa atgctatctt gaaaacgttg accattctgt gagaaattat
    10261 cagtagaaac catcgcaatc ggtaactctc cagcattaaa cctgctatac aacaaggatg
    10321 tcacaatact catcgtatgt tgaggattgg taggtccaga ttttatatct gcttccacta
    10381 ccgaaaaaaa ttgtccttta gaatctttta aactgtatcc tttttcagta atagtaaacg
    10441 ttgtaaactg taatgtagga tcttcaaaat atttaaccac tctttgatag tccgttatat
    10501 tctttggatt gcaatataaa ctatcagctg ttactgctaa aagtcttttt ttgagttcac
    10561 catcttcacc cataacaatc tccaaaatat cattgttgta gtcatgataa gctttttcta
    10621 ctacttcttc atcaaatgtc tcacacacaa ctactccgga ttctaattca ttttgatctg
    10681 ctaagcattg agcaatttct gcatgaaatc ctcgataaag atttcctcca ccaaaatgaa
    10741 gccacactgg atgtttctga ccttcttgtt tgaccgcttc gacattaaaa tacggtagtt
    10801 gaacttgcat tttgttcaat ccttcgatag aatcttgcca gtctttaatc aaaaccatat
    10861 ttacccctcc atactgatat gttagtttcc gttacaattg tagtataaaa tcgaatgtaa
    10921 tcgctgtcaa taagaaaaaa ctaaaaatat tcttttttct accatgtcag aattattgtg
    10981 tcttttttta gtaaaagcac accaatatat attttttaga actaacttaa attaaagctt
    11041 aatttctctt tgatcttatt tgagtatttt ttgttttttt aatagctagt atgttacttt
    11101 gaaatttgat attatataac gagcatagaa ctaaaatatc aaattcaaaa atgaaatttt
    11161 gagagggatt aaatatgaca attaaaacta gtctccaaaa tcaagcttat caatctattc
    11221 gtcagcaaat tatctacgct gacttagaac ctggaaaaaa agtatcagaa aaaaaattgg
    11281 aagaaacgct ttctatcggt cgtactccaa tacgtgaagc attgattcaa ctccgtcatc
    11341 aaggacttgt tgatacaatt cctcaaagtg gaaattatat ctcattaatc gacttagatt
    11401 tagcaaaaaa tgctcgtttt gtcagagaac atttagaacg acaaattatg ctagagtgtt
    11461 gtgccaagat gaataatcaa aagaaacaag ttttagaaac tataattgca gaacaagaaa
    11521 aagctgttag tcaaaagaat gctcgtagtt tttttcataa tgataattta tttcataaag
    11581 cttgttttga aattgcagaa cgggaagaag tatggcaatg gttggaagat aataatacgc
    11641 acttggaacg atttcgttgg ttacgagtaa aaacggaagg attaaaatgg gaaaaaatta
    11701 tggaacaaca tcatcaacta tttaaagcct tgaccatgca agaccctgaa gaagctaa
//
LOCUS       DADOOS010000083        11667 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.083, whole genome shotgun sequence.
ACCESSION   DADOOS010000083 DADOOS010000000
VERSION     DADOOS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.083"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(37..216)
                     /locus_tag="J8C37_001876"
     CDS             complement(37..216)
                     /locus_tag="J8C37_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305311.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="J8C37_001877"
     CDS             complement(597..1304)
                     /locus_tag="J8C37_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305312.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="J8C37_001878"
     CDS             complement(1297..1734)
                     /locus_tag="J8C37_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305313.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="J8C37_001879"
     CDS             complement(1749..2000)
                     /locus_tag="J8C37_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305314.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="J8C37_001880"
     CDS             complement(2000..2206)
                     /locus_tag="J8C37_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAZ5305315.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="J8C37_001881"
     CDS             complement(2643..2903)
                     /locus_tag="J8C37_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305316.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="J8C37_001882"
     CDS             2978..3181
                     /locus_tag="J8C37_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAZ5305317.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="J8C37_001883"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="J8C37_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ5305318.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="J8C37_001884"
     CDS             complement(3810..4709)
                     /locus_tag="J8C37_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAZ5305319.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="J8C37_001885"
     CDS             complement(4722..5315)
                     /locus_tag="J8C37_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAZ5305320.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="J8C37_001886"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="J8C37_001886"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ5305321.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="J8C37_001887"
     CDS             complement(7771..9231)
                     /locus_tag="J8C37_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAZ5305322.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="J8C37_001888"
     CDS             complement(9292..11664)
                     /locus_tag="J8C37_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305323.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DADOOS010000084        11643 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.084, whole genome shotgun sequence.
ACCESSION   DADOOS010000084 DADOOS010000000
VERSION     DADOOS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.084"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            316..675
                     /locus_tag="J8C37_001889"
                     /pseudo
     CDS             316..675
                     /locus_tag="J8C37_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="J8C37_001890"
     CDS             complement(970..2103)
                     /locus_tag="J8C37_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ5305324.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="J8C37_001891"
     CDS             complement(2094..2762)
                     /locus_tag="J8C37_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5305325.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="J8C37_001892"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="J8C37_001892"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAZ5305326.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="J8C37_001893"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="J8C37_001893"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAZ5305327.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="J8C37_001894"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="J8C37_001894"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAZ5305328.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="J8C37_001895"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="J8C37_001895"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAZ5305329.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="J8C37_001896"
     CDS             complement(6138..6911)
                     /locus_tag="J8C37_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ5305330.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="J8C37_001897"
     CDS             7076..7555
                     /locus_tag="J8C37_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5305331.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="J8C37_001898"
     CDS             7612..7914
                     /locus_tag="J8C37_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAZ5305332.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="J8C37_001899"
     CDS             7936..9393
                     /locus_tag="J8C37_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAZ5305333.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9440..9667)
                     /locus_tag="J8C37_001900"
     CDS             complement(9440..9667)
                     /locus_tag="J8C37_001900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305334.1"
                     /translation="MRSDSGKKKSRNSQLNNKKSKSTKQTRTQKESLRNTNKGKLKGV
                     KKSINVDNFFIGCGKRWAMLFEMDFTSIYFL"
     gene            complement(9986..10738)
                     /locus_tag="J8C37_001901"
     CDS             complement(9986..10738)
                     /locus_tag="J8C37_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ5305335.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="J8C37_001902"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="J8C37_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..>11643)
                     /locus_tag="J8C37_001903"
     CDS             complement(11199..>11643)
                     /locus_tag="J8C37_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5305336.1"
                     /translation="HKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHL
                     FKSMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDF
                     RDMDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         3343 a         2510 c         1994 g         3796 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc atttagccct
    11401 ctttgcgaag gtgttcctgg atctgcgaaa aagatggcaa tatcgttcga gttacttata
    11461 gatcgccagt tggaaaattc ttttccacaa tcaaatgtca tggatttaaa caagtgttta
    11521 ggcatggatt tgaaccaatc atttaacctg ttttcgatat cttcggctcg tctgcctgtc
    11581 ggtttcaaag taataataac cttggataaa cactcaacca atgtaataac agcgctcttg
    11641 tgt
//
LOCUS       DADOOS010000085        11590 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.085, whole genome shotgun sequence.
ACCESSION   DADOOS010000085 DADOOS010000000
VERSION     DADOOS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.085"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(192..737)
                     /locus_tag="J8C37_001904"
     CDS             complement(192..737)
                     /locus_tag="J8C37_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ5305337.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(749..1441)
                     /locus_tag="J8C37_001905"
     CDS             complement(749..1441)
                     /locus_tag="J8C37_001905"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAZ5305338.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1460..1744)
                     /locus_tag="J8C37_001906"
     CDS             complement(1460..1744)
                     /locus_tag="J8C37_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305339.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1757..2320)
                     /locus_tag="J8C37_001907"
     CDS             complement(1757..2320)
                     /locus_tag="J8C37_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAZ5305340.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2548..3210
                     /locus_tag="J8C37_001908"
     CDS             2548..3210
                     /locus_tag="J8C37_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAZ5305341.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3334..4530
                     /locus_tag="J8C37_001909"
     CDS             3334..4530
                     /locus_tag="J8C37_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAZ5305342.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4631..6007)
                     /locus_tag="J8C37_001910"
     CDS             complement(4631..6007)
                     /locus_tag="J8C37_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5305343.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6044..6910)
                     /locus_tag="J8C37_001911"
                     /pseudo
     CDS             complement(6044..6910)
                     /locus_tag="J8C37_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6999..8024)
                     /locus_tag="J8C37_001912"
     CDS             complement(6999..8024)
                     /locus_tag="J8C37_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5305344.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8042..8716)
                     /locus_tag="J8C37_001913"
     CDS             complement(8042..8716)
                     /locus_tag="J8C37_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5305345.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8900..9793)
                     /locus_tag="J8C37_001914"
     CDS             complement(8900..9793)
                     /locus_tag="J8C37_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ5305346.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9867..11564)
                     /locus_tag="J8C37_001915"
     CDS             complement(9867..11564)
                     /locus_tag="J8C37_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ5305347.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3456 a         2525 c         1981 g         3628 t
ORIGIN      
        1 aattataata agtgtatttt ataaaaagaa aaaatataac ttaagttgtt gataataaag
       61 agatttatta acgtaaaaag aataagtttt tttaaaataa cgtataaaaa agagcataaa
      121 acaaagtcgt ttgttttatg ctcttctttt attattttgt gctttttatt ttatcaaatc
      181 actttgtttt tttataaaat ttctgcgcct agtgtggact caaaatgtct caatgcccat
      241 tcatgtccaa cgggatcgaa acttgccacg gcatctttga aaatatggag tttgtatcct
      301 agattatacg catcgactgc tgtatgcaac acacagatat ctgtacatac gccggtcaaa
      361 taaatatctg tgatctggcg ttctcgcaaa cggatgtcta gatctgttcc actaaaagcg
      421 gaataatgcc gtttgtctat ccaataaaca ttttcctctt gctcatagtc ttggtataaa
      481 ctttttaaag agccgtacaa ttcccgtcca cccgttccta tcacattatg cggaggaaat
      541 aagcggttct ccggatgaaa ggaatctttt ggatcatgcg catcaatggc aaaaacgaca
      601 aaatcctttt gatcaataaa cttctttgta tgagcagtca gctttttttc aattacttgt
      661 cctgctgtcc cagtagtcag ctttccatcc tctgcaacaa aatcatatgt gtagtcaatc
      721 gagatcaatg cgttcatctt agtcctcctt atacgagatg tttgacaaat tcgatcacca
      781 tttctgctga tcgtttgcct gcgtcttcga taaattcgtc aaagctttgt gtcgccgagt
      841 gatctgctgt gtcgctcatt gcacgtacga tcaaaaatgg gatattgaac tgacgagctg
      901 tttgtccaac agcggctcct tccatttcgc aagctaatgc ttcagggaaa ttcgttaaga
      961 tttctttgat tttatctggc gagtcgacaa atgtatcacc tgtaacgatc aatccttctt
     1021 ttgcgttcaa attcgttgct tttgctgctt taaccatttc actgcgcaaa tatgtgcttg
     1081 cttcataata tagaggcatt cctggtaatt gtcctggttt gtagccaaat cctgttgcat
     1141 ctgcatcaaa ataagctact ttgtctgata taacgatgtc acctacttgt aagccttcac
     1201 cgattccgcc agctgaacct gtattgatca ccatgttcac gccgtattgt tggatcaaca
     1261 gacttgtggt aatagatgct aagacttttc ctataccgga acgtacgacg atcacttcgt
     1321 ggttacctaa tgaacctgaa ataaataaag cgcctgcgcg ttcccatgat aatggttcgc
     1381 ttaggttctc tctcaaaatt tttacttctt cttccattgc gccgataata ccgattttca
     1441 tctttttaat tccttctttc tatataaatg cgattgctaa aaaaaccaca cataacatta
     1501 ggataacaat aataagagat ttcatcaaaa aagagttcca tttggtcgtc ttttcccgtt
     1561 cgctgatcct tgttttcgtg attggtttat tctttttgct ttctttacga taaaagctgg
     1621 caatcttttt ttcttcttgc tgataagctg tttctgcttt tcgctgtttt tttaatgact
     1681 cgcttgcttg tgcttgttgt cgcttgcgaa gttcgcttcg cgtaataagc ggtcctttgg
     1741 ccataagccg atccctctac tttcctttgg tcaatagttc ccagtattga atcgcataaa
     1801 tcgtctttgc atcacaaatc aactgatctt tcatcgcttg ttgcgcctct tcaagagtta
     1861 gatgatacaa ctccagtact tcgtcttcat cttgtgctaa aggattctcg accttctcta
     1921 ccccttgtgc atgataaata tgaaggactt catttgcaaa acctggtgaa agatacatcg
     1981 aagtaagatg gctcagcgac ttcgcacgat agccagtttc ttcttctaat tctctagctg
     2041 ctgtcatttc gggattttgt ccttctcctg gatcgatttt tccagctgga atctccaaaa
     2101 tcactttttc taaaggtttt cgaaattgtt tgacaagaag cagacgatct tgttcgtcaa
     2161 aagcaatgat cccgacacca ccaggatgaa ataccagttc tctcttcgaa gttcctccat
     2221 ctggcaaacg aacttcgtct acagcaacat caataatttt tccgctatag atttctttgc
     2281 gactgattgt tttttcttca aattgctcat tgttcagcat atcttctcct gctttctttg
     2341 taatcataac tgcttttttt tcatttttca atttttctca aaaactcctt attagtttac
     2401 catagataaa ccttagattt tctctaaata tagaaaatcc ttctttagtc cgatactctt
     2461 tctagtagtc cgtatgctaa aataagtatg taatcgaata acgaaaagaa ttcaatactc
     2521 aacgataaat gacatggagg gtttattatg ataaaacgaa attggccgtg gcttctagca
     2581 ttggtcttgc tatttctctt tgtccgaaac aatagttttt cttttgttct aggactttta
     2641 ttgataggtg tagttttagt tttgtgggga ctctttggtt cgcgcaggaa aaagggaaaa
     2701 caagaagaaa ttcctgcact gactgacgaa cttgaaactc actatgaaaa aagcggtatg
     2761 acagcgaatg aaatttcatt ttttcgacaa acaatgaatc aaacaaaaaa tgagatcaat
     2821 caattgcaaa tgaatatgca gcagacagcc aaacttaaag caatcgatct ccgccatgat
     2881 ccagtcaaag cagcgaaagc tttattcaaa gaattggtga aagaaccaaa ccgactgcat
     2941 gaagcaagtc agtttcttta tacgcatctg ccgaatctag ttgatttgac aaataagtat
     3001 aatgagatca atgaccatga gatcaaaaac aaacagacct atgaaaaact agaagaaagc
     3061 gcgcagatca tcgaccagct gtcagcttta atcgcacaag attatcaaaa attcgttgct
     3121 gaggatctag atgatatcga cgtagagata tctgttgcga aacagagctt aaagcgtgat
     3181 aataaatatg aaccagaaca aaaggattaa ctcttttgtt cataaaagaa gactcggaac
     3241 attcagatga tgtctggctg tttcgagtac tttttctaaa gtagagacat tcatgtaaga
     3301 atcgagaaca gattcaacaa ggaggaattt tttatggatc ataaaccgac tgaaacaaaa
     3361 gatgttacac cagttaacga tacacttgaa gatttattga acaacccgtt ttcaactcca
     3421 acagatacgc tgaccacttc acaacaagcg gagattgatc agctccaaga gcaacagaca
     3481 gctgctcgtt taattgacaa actaccggcg gaacgccaag aacaagctaa acagctagca
     3541 gcaaaaatcg atgctaatga tgcacagtct gtcatcagct acggatcggc tgcccaagca
     3601 aaattaagtg aattctcaca atcgatgttg aatcatgtgc aagcacaaga tattggtcct
     3661 gtaggagatt ccttaactga actgatgtat cgtctccaag aagccaatcc tgacgaactg
     3721 cgtgccggag aagggaacat cttccagcgt gtcttcggga aggtcaaaca atcgatctat
     3781 gaagtgacag caaaatatca aaagattggt gcacaaattg acaagatttc agtgaaatta
     3841 gataaagaaa aagacggtct gctaaaagat aatctgatgt tagaacagct ttaccagaaa
     3901 aacaaagact actttgatgc actgaatatt tatatcgctg ctggagaatt gaaaatggaa
     3961 gaacttcaaa caacgatcat tccagaagcg atgaaaagag cagaagaaac tggcgatcag
     4021 atggatgttc agattgccaa tgattacacg caattcctag atcgtctgga taaacgtacg
     4081 catgatcttc gcttagctag acagatcacg atccagcaag caccacaaat ccgtttgatc
     4141 caaaacacaa atcaggcctt ggctgaaaag atccaagcat ctatcgctac agccattccg
     4201 ctgtggaaaa accaagtcgt gatcgcattg accttgcttc gtcaaaaaga tgcagtaact
     4261 gctcaacgcc aagtctctga aacaacaaac gacttattga agaaaaattc agaaatgctg
     4321 aaaatctcag ccatcgaaac agcgaaagaa aatgaacgtg gaatcgtgga tatcgagacc
     4381 cttcaaacca ctcaaaatga tttgatcgaa acaatccaag agacattgcg tatccaaaaa
     4441 gaaggaaaag aaaaacgtcg tcatgcagaa gtagagcttg gacacatgga agaagatctg
     4501 aaacaaaaac tgttggattt aacccaatag atcacctttc gcttgttact aaaacaaacg
     4561 cccaacaaca gtttattaca actgttgatg ggcgtttttc ttatctttct tctaacttct
     4621 ccattggagt ctatttctca ttcatcttgc tgtgtccata gccatagcca aaataaatga
     4681 tcagtccaat aaccattacg ataccgaaca cgatccatgt cacagtttgc agacgaagca
     4741 tcaatacaat acaagtcaag atggagacga ttggcaatag cggaacgaat ggcactttga
     4801 attctccagc ttttggtttg ccaaacattt ttctcaactt cagtaagcct agactaacaa
     4861 gcatcaaata ggcgagcgcc atgatattcg ttagttccgc taatacattc aatgggaaaa
     4921 atccggcaaa gaatacagct gccacaccag cggcataagt cgcttttttc ggtgtacgat
     4981 gcttttgatc gatttctgtc ataaacttag gaagtaagcc atccttgctg acagcaaaca
     5041 gtaaacgagc aagtgaatac atcatcgaga tcgttaccgt cagtagcgtt aaaattgccc
     5101 ctacagcgat caccgcgccc actgttccat gtcctacata cctcattgca aaagccactg
     5161 ggtcattgac tcctagttca gtatacggaa cgattcctgt caagatcagc gtcacgatga
     5221 tgtaaaggac agtagcaatc agtatcgaac caatgattcc tcttggcaca tctttttgcg
     5281 ggtttttgac ttcctcggct gccattgcaa cggcatcaaa tcccaagaaa gcaaaaaaca
     5341 caagagctgc accatctagc acaccggaaa aaccaaaagg catgaatggc tgccagttat
     5401 caggtttaac gaaaaatatt cctacaatga taaacaacgc gatgatacca aatttgacat
     5461 ataccatggc attattcaaa cgtaatgctt tcttggcttc aagcgacacg atgtaagtga
     5521 cgaatatcac tacgagcacc gcaatcaagt caatgtatgt accattttcc ggattgtacc
     5581 cgccagttaa tgcttgtgga agttcggccc ctaaactttt caaaaaacct tgcatatagc
     5641 cagaccatcc ggaagcaaca gaggaaaccg ctagtaaaaa ttccccgatc aacaaccacc
     5701 ctgtcagcca ggcagcaaat tcaccaaata caacatacag ataggcatat ggcccaccaa
     5761 tcactggcac tcgcgaagca aattctgcga aacttagtcc agacaaaatg acaacgattg
     5821 cagctaagac aaatgaaagt gacagtgctg gaccagcatt ttgattggcc gcaacccctg
     5881 tcaccacaaa tattcccgta ccgatgatcg caccaattcc aagcatgatc aaatcagacg
     5941 tttttagttc ttttttcatc tcactcgtat gattgagatt gatctctttc ttacgaaaca
     6001 aatccattga agttcctccc cattttaggt tggaaaacag cgattaaact gttttcatat
     6061 accctaaaag gataccacct aaaatgacta aaaggcaacc aacaataaca taaatgagct
     6121 cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag ataatgatcc
     6181 ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt agaagcatgc
     6241 agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca aactgatcga
     6301 ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata ctttgaggta
     6361 agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat acaccataac
     6421 caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt ttattatctg
     6481 tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt acaagaagag
     6541 ctaaagcaat aataccctat acataatctt tcgtttgtgt ccattcatgg aagaaaacgg
     6601 ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc cctacagaaa
     6661 cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa gctaagccgg
     6721 ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg aaaaagacaa
     6781 ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg gcatcgccac
     6841 caattttacc acttactaaa ccaatactac cccatgccaa catagggact aatgctatta
     6901 aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca gcgaaaacgt
     6961 taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc attataaaaa
     7021 tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga ttcagccgtc
     7081 aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa tcctgttgaa
     7141 tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat ccgtttcaag
     7201 tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc ttgcgcttcc
     7261 ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc attactaagc
     7321 aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg atcctcacca
     7381 acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg tatcacaggc
     7441 tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa actgtcgagt
     7501 cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc attttctagt
     7561 aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa tttagaagcg
     7621 gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg gtcttgttga
     7681 ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt gatatattct
     7741 tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa aggggaagtt
     7801 ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc acccagcaga
     7861 taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct caaatcaact
     7921 ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa cagccatccc
     7981 aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac cgctcatcct
     8041 ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg atacctgcct
     8101 gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca tctacaaatc
     8161 gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt ccattgtgct
     8221 tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc tctccgttga
     8281 tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc atttcactag
     8341 ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca atcagtacat
     8401 cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc atgatcatat
     8461 ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact tcacgtattt
     8521 tttgacacca ataatagcca tccaaaacgg gaagattaat atctagtaat acaagctgtg
     8581 gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca aaggtttcaa
     8641 attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg tcttcgacta
     8701 ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca atatcaaaga
     8761 agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac gttaaaaaaa
     8821 actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat ttattatgtt
     8881 attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt cttttaaatt
     8941 ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga aggagtataa
     9001 gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact tcttctgtaa
     9061 agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa ggtgtacaag
     9121 tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt tttgttggaa
     9181 ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact tcaagataga
     9241 atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca gcaataccgc
     9301 tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag gtgccttgca
     9361 ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa ggcaagctaa
     9421 cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat tcttttcctg
     9481 gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc attcctggga
     9541 ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat tgttgctcat
     9601 gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt ctttgatcca
     9661 aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct ccggcaagaa
     9721 acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt ctattaccta
     9781 ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc tttataaaac
     9841 cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga tttacggtac
     9901 cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa gagcccgttc
     9961 ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg ccctggattt
    10021 tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat aaattcagta
    10081 aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc tttcatttga
    10141 taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc gacaaattct
    10201 gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata aacagctaaa
    10261 ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca caattcgaat
    10321 cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt tcttccacct
    10381 gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc atttcgtccg
    10441 atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt ggaaaccttc
    10501 aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc taaagtatct
    10561 actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt gcttactact
    10621 tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac ttctatttcc
    10681 tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt tttgataaca
    10741 gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt gatcgtgtaa
    10801 gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc tccagcttca
    10861 ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt atctgcttct
    10921 ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt ccatgtagaa
    10981 ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac aaccattgga
    11041 gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata gactttatct
    11101 ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc tccagcttca
    11161 ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact ttctgtgtgc
    11221 cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg ttccggtgtt
    11281 aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa atcaaaatcg
    11341 actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt cggtcgttct
    11401 tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact tggtttttgt
    11461 gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc cgcagtaacg
    11521 agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt ctccccgttc
    11581 tttttttatt
//
LOCUS       DADOOS010000086        11405 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.086, whole genome shotgun sequence.
ACCESSION   DADOOS010000086 DADOOS010000000
VERSION     DADOOS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.086"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(99..1562)
                     /locus_tag="J8C37_001916"
     CDS             complement(99..1562)
                     /locus_tag="J8C37_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAZ5305348.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1626..4643)
                     /locus_tag="J8C37_001917"
     CDS             complement(1626..4643)
                     /locus_tag="J8C37_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ5305349.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4794..5633)
                     /locus_tag="J8C37_001918"
     CDS             complement(4794..5633)
                     /locus_tag="J8C37_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5305350.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5778..6023
                     /locus_tag="J8C37_001919"
     CDS             5778..6023
                     /locus_tag="J8C37_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305351.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6086..6508
                     /locus_tag="J8C37_001920"
     CDS             6086..6508
                     /locus_tag="J8C37_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305352.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6602..6919)
                     /locus_tag="J8C37_001921"
     CDS             complement(6602..6919)
                     /locus_tag="J8C37_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305353.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6946..7152)
                     /locus_tag="J8C37_001922"
     CDS             complement(6946..7152)
                     /locus_tag="J8C37_001922"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305354.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7133..7315)
                     /locus_tag="J8C37_001923"
     CDS             complement(7133..7315)
                     /locus_tag="J8C37_001923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305355.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7329..7679)
                     /locus_tag="J8C37_001924"
     CDS             complement(7329..7679)
                     /locus_tag="J8C37_001924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305356.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7705..8007)
                     /locus_tag="J8C37_001925"
     CDS             complement(7705..8007)
                     /locus_tag="J8C37_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305357.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8010..8243)
                     /locus_tag="J8C37_001926"
     CDS             complement(8010..8243)
                     /locus_tag="J8C37_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305358.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9229..9551)
                     /locus_tag="J8C37_001927"
                     /pseudo
     CDS             complement(9229..9551)
                     /locus_tag="J8C37_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9674..10789)
                     /locus_tag="J8C37_001928"
     CDS             complement(9674..10789)
                     /locus_tag="J8C37_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305359.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10795..11193)
                     /locus_tag="J8C37_001929"
     CDS             complement(10795..11193)
                     /locus_tag="J8C37_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305360.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3008 a         2348 c         1757 g         4292 t
ORIGIN      
        1 tcaaattaaa aaacgaaaat gagagataca aatatatcaa atatttaaaa agtaaccaca
       61 ttttctatac cactccttat cagaaacagc cttattcttt agaataagac tgtttctggg
      121 ttgattaatt tgtcttttgt taaactacca tctaaggcaa gttctaaatg taaatgtggt
      181 cctgtactat ttcctgttga acccacataa ccaataattt gtccttgttc aactgtttgt
      241 cctacgttca cgcaatattc ctgttggtgc gcataaagag ccacacttcc gtcttcatgc
      301 tcaattgtga cataatttcc tgacaaagta tgatacgaag cttctatgac cgttcctgcc
      361 ttgctcgcat agattggttc tccttgattg gctgccatgt ctaatcctct atgaaattct
      421 tctcctcgcc aaccgaagga actagaaatt gaataatttt ttagaggtac catatagcct
      481 tctttgctta gttgtggacc agctacttct ttatcatatt tgattaagtc ataggtttcg
      541 attaacccat ttaatttttt atcgtagcta gtgtctgttg cgtatcttcc tgttaaaaat
      601 ttagtcgctt cctgataggt ttttgcctta cttttccaaa caccatcata aaaagaggta
      661 tttccaacta agccttcttt taacagtttg gcatagtctt ttaagctttc ttcatagctc
      721 tcatattgcc gaaaagttgc ttgggtcgta taaagtgtcc cattgcctaa atcttcttgt
      781 gtcgcaaaag agacgccctt tccgttatgt gtgcctttga ttccaaatag attgtagttt
      841 ggtgcctgag ccaattgact ttgtccgctt gcggattcta aaatggcttg cgcgatcata
      901 acagaagcgt ataaatcatt ttcttgtcca attttacgag cagattcacc gattttacgg
      961 ataaacgttt ctacagacgc gtctttttca aaatgaatag tcccttcgtt atcttgtgtg
     1021 ctggaaggga ctaattcttg tatcggtgcg cttggtgctt ggtggtagtt ttttgtctct
     1081 tctgaaccga ttgattgtgc ctgcggtacg tcattcatag gtacagtcac actctgttca
     1141 ggtgcctttt gtgggcgttc cgtttttgtt ggcggttccg cttctgtttc ggtacttggt
     1201 ttttgaggag tctgtgagct ttcatcagaa ggttgctcat ttggttcttc tgacggttcc
     1261 tcctcaggtt gttgattgtc ttctggtttg tctggttgat tcggtgtttc tggttgttct
     1321 tcagaacctg ttggtggagc gttcatttct ggattatctg gtgtatctat tgtatcttca
     1381 gtcggtgttt ctggttgatt ttcttctcca gtggaatttg ttgtcgtttc ttcagtgatc
     1441 gttgcagatt gcgaatttgt cagttgattg ttgccttgtt cattagccaa aaccgctggc
     1501 gtatacccaa atacttgacc tactaataac aaagcagata aacatttact cgtttttttc
     1561 acaagattgc ctccattctt caatcaatca tctagagaca gctgattgct gtctctagtc
     1621 atactttaat tgctttgttt cattttacga cgaaccagaa cagcccccac accaacaaaa
     1681 agaaacagtc ccaatgcaat taacacatcg ttcactgttt ctcctgtttt tggtaagctt
     1741 tgttttggtg tgctcgtcgg cgtttgtttg acaggggttg tcggcgtttc ttttttaatg
     1801 ccttttgctg tcaatgtttg tttttcattc ttcaaatcaa agtgacgtgc ttcttcctct
     1861 ttatggtctt tatccttata gacaatatga gttgcgacca atttctcccc gtcttttaac
     1921 gtgcctgctt tgtagtcaaa ggatacttta aactcttcag acgctttaga aactttacgt
     1981 gcagtttcta ccgtaccaac gactgtatct tttccatttt tatcaatctt atggaattga
     2041 gtcacccagt atttggtttc ccctaccgta gattctggaa attcttgcga tacatgatcg
     2101 atcaactctt ggtctttgtc tggatcaaat gtttggtcgc cattcactgt aataaagcgt
     2161 gtttcgattt ttggttcttt cgcagtcact gtttgatctt tattggttaa atcaaaatga
     2221 cgagcgaatt cttcttcctc ttctttgtcg ttatagacaa tgtgcgttgc gacaatcgtg
     2281 tcaccgtctg aaaccatgtc tgctttgtag ttgaacgtca catcaaatgt ttctttttca
     2341 tcggtcactt ttcgtgtttc ttttaccgta tctaatacag tgacttttcc atcggcagta
     2401 attttatgga attgcgtcac ccaatatttc tctgtcccaa ccgcagattt agggaatact
     2461 tgggacactt tatccactaa ttcttggtct tttttcgggt tgaaatcttt tccacctgtc
     2521 gttgtcacaa acaaagtaga aatttctggt tggtcttctt ctggaaccac gatttcttcg
     2581 tttttcacag taattggttg tacttctccg tctttgaccg ttactaaaat cgtttctgtt
     2641 tgtcctgacg cttgcccata atgctcaggg aacacaattt ccgtaaattt atacgtaccg
     2701 tctggtaatt ttagtttttc tggttcttct tttgtttcat acacaaagga cacattttta
     2761 ttatgtgcag tcataccttc aatcaggaat ttcgctcctg gtaattgtag atgattgatt
     2821 gaatcgactt tattgaagtc tacttcaccc gtttttaaca agttgtcaat cacatctaat
     2881 tcaaacgtaa gcacaggcgt cgtgttatcg ccataggcca cagagaacgt ggtttgttct
     2941 tcagaaggtt gacggtagtt gtctggcaca tttttttcaa tcaattggta tttgttttga
     3001 tcgccattgt agaatttttt gtttgccttc cagtgtccgt ctttgtcagt ttttactgaa
     3061 tccaagactt ctcctttttt cacaaggact gtttctttat ctggcgcagt aatatctgtt
     3121 ttggcgataa tatcaaattc gatattttct gcaggaacgg tatcataaac aggtttgcct
     3181 tgattccagg attcttccgc attatagact tctttgactt tctttccttc tggaatgaac
     3241 tgttgtaagt cattttttgc agttaacgtc acttctttgt ttggttcaat cgtaatcgta
     3301 tgagtttgtt tgtctagaat atacccagct ggtgctttga cttctttgac atccaattta
     3361 ttttgaacat attttccgtc tttttcatag aatgcaggga tttcttttaa ggcgatttcc
     3421 cctttggcat ccgttgtata ttgttttgtt tctcccgtat catgacgttt gatttcatac
     3481 gtcacatttg gaactttttt ccctgtgaca gcgtctagtt tggttagttt gaagtcgcca
     3541 ttttttagaa ctttaatagt tagtccagct ctattaggat ctggcacata aagctttcct
     3601 agattttgag caacaggatc tacataggca aaacttgaac ctacccctct agaatttgga
     3661 tccagtgaag aaacagaaat tcgtccgtca tttgagtttt gatttgcttt tataaacaaa
     3721 ctatttcctg attttcttac ttgaacgtta gctgtgtttt caacaaccga cccatcccat
     3781 tgagaaaaca caccttttgt atccttaact tctacttctt cccctacttt aaccgtcact
     3841 gtttggctat caaaagaagg atcttcaagt aaattgttta tttttctatc tacagtctgt
     3901 ttgaaagctt cataattaga ttgtccaata gttccataat taacaaatga accacctaaa
     3961 acttcccaaa tatatgtttg tgctaataca tagttatcca ttgaaccact agaatagtag
     4021 ccaaaataac caattaaaga aatctttttt ctaatagcac ttgtcgtaat taaattttcg
     4081 agtggtgtca catcatattg aatacccgca ctcgtgattg cgccttcttg aatgcaaaaa
     4141 acatcttctc cgtcaatttg ccagcgaacc atgtaatcat gagtatacat tgttccttca
     4201 ggagtaattc gagtcatatt cacatcttta cgaccatttt cagtcaatgt tagattggta
     4261 gcacgtgtct gtgcattctt ttccaagatt gcttgccatt cttcagattc ttgcgccaat
     4321 cgttcttgtt cagaaaaaga agtcgctttt tgaatatctt caatggttat aaagtatttc
     4381 tcataacctt cagggacctc ttgagtcatt acattgggcg gagaaacatc ttctgtcgtg
     4441 tcctgcgtag ttgtctcttc tgttgtttca cttgtggttt cagtactgat cgcttcttca
     4501 ctactttttg attcttcagt cttcattact tcttcagtag aatttgattc atttatcgtt
     4561 tctgcaaatg ccgttacaga tcctaacatg ggattgagaa atgtaacgaa aattgccaac
     4621 caagttgcca ttcttttaaa catatttttt tcgttccttt cattttattt ttcagtcttc
     4681 taaagtaatt tcttatggac ttcgacaccg aaacattttt atcctcctca aaacaaaaaa
     4741 agactagaac ttataattct agtcttcatc aaccacttac ttttctttta tttttagtag
     4801 aaatctacag tgtgtttttg tgacccgtca ctgtaaataa taacatccac cataaaacct
     4861 ttgttgtacc actgcgaatt tggatcgcta acctctgcat tagcccaatc ataggcttca
     4921 tctacagtgt taaatacctt tccactatta ccaatctctc caactacaat tgttggctct
     4981 tcaggtgtcg ttgtgccacc atttccgcct tgatcgcctc caccaggatt gcttggttct
     5041 tcaggcgttg ttgttcctcc acctgtgctt gggttagaag gagttgtttc tcctccagta
     5101 tttccaccat tacctggttg tgtaggtgtt tgagtagtgc caccattgtt ttctggtgta
     5161 cttggcgttg ttgtaccacc agttgttcct gtttgatctc cagctggtgt tgttggtgtc
     5221 gttgtatttg gtgtcgtttg tgtatttgaa gtcgttcctt gtcctactgg ttgattcgga
     5281 tcaactttgt ctttctcttg aatcactgtt tgagccacag tttcacctgc gctgttttgt
     5341 acacttgcta cattgccatt aaatactaat ttatttcctg catagattaa atccacattt
     5401 gtaatcttat tcatgtccgc taatttttgg acagtaatat ttgttgcttg actgattgcg
     5461 cttagggtat ctccccagac aattgtgtat tcattccctt taatttctgc tttgatttgt
     5521 tccgccgaac gaggtgtcca aactttgttt tctgctgctt cagcattcgt tcccacagca
     5581 agactaactc ctccgactac gattcctagt gccaatactt tcaacatttt cattgttttt
     5641 cgcctccgat ttgaataaat ttatttacaa aaacatagta ccaaacttat gtctatcagt
     5701 cgatatattt tttgtagatt tttttatttt ttgatatact aactttggta ctaaaataac
     5761 taaaaggaat gtcacacatg caacttaata atttaaatga aatacatgat tgggctcaaa
     5821 ataatttact taatcgtaaa caagctgcca aaatcactgg acaaaactat gatgcttttt
     5881 ctagctcttt acgtagaaat aatatcaaac catttctaga aataggcgac gaacctggtc
     5941 cttccatcat tcgcttatat ctaagagaag atattgaaaa atacgcaaaa cagctcgaag
     6001 aaaaaagaca gaaacgtcaa tgaggcgttt tctgtcttgg atttacaagt aaaacaataa
     6061 taaacacttt tacaacggag gttctatgaa taagaaatac attgtttcag ttggttcaat
     6121 cgtactgatt gtactcgcta ttattgcttt ttctgtatcc aacaaaacag aaacacagcc
     6181 acaaaatacg gttcaatcga ctactacaag tcaatctagc gatcattccg tctatccttc
     6241 actaaatagt ttaaaagaaa cgcatggaca agatttaatt gatcttggtt cttctcaaat
     6301 tactactgtc ttatttgaaa agaaaaatat tgcgtttatt actcctaaag aaggaactga
     6361 ccttgaattt tctatcaaag taacaaatac caaagaaaat ttattctcca ttcctgacat
     6421 tcaatatacc cctaacaagt taaagattgg ggatacgttt ggtttggcta aagtaaatga
     6481 acaatacttt gcttactcac tgaaataagt ttctaatagc aagaggaaaa accctcttgt
     6541 tatttttatc acataatcat tttataaaaa cgcttcgaaa atatcaacat ttttgaagaa
     6601 actatttttc taatttctca atttcttcca agggattttc gctaatgacc catacaggca
     6661 cagattgctc tcgaggctcc ccttcttcta accaccaacc tgcataatga acttcttcct
     6721 cctcagcatt accatgatac caaccaacaa aggattcatc tacaacgaat gaaatttctt
     6781 cttctggatc aaataattca tttttccatg cttcaattgc ttgttctttg gtgtactttt
     6841 gtttgttaaa tacaccacaa atttcgtcat ctacataaaa aatttcaaaa tcaaaattac
     6901 tcgttccttt tactggcatc ttaacttttc ctcctttgac tcacttcaat agatatacca
     6961 ctcatgttca ttccattgtt tactagttaa ttggagtttt atcttagggc tttgtgcact
     7021 ctttccaaaa acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat ttcttacatt
     7081 cggtactaaa tgtttttcct gcatttcttc atgaaaaaca tataattctg cttcattttt
     7141 caaccaattc attattcatt ccctcattca cttcttcata ccgccattca gatagtgaga
     7201 tttctttttg tttctccact tgtcctataa aaatcgctgt attcaacaag ctgatcacac
     7261 ttaataataa agcaatgaaa ctaatggtta cacttactct ttcacatttt gtcattttgt
     7321 ttccttcttt actcgtttgt tatttcttgt aataggtcta ataactcctt caccgaaatt
     7381 tgcaaatggt tcgcaaaaat ttcaataaaa gcactcgcac acattaccag accttcaact
     7441 gatatatcac tgcgacaata aaattctgga gaaacactga tttctgattc tttcttacaa
     7501 ttgaacgaaa aaactatttt tgaatcaaat tccggtaatt cttccacaac tatgttttgg
     7561 gattctacca agaaagggag tgcctgttct aatgcttctt ctggtgtttg agcagataga
     7621 ataattgctt cttgttcttt tatttcattg ttctcaaata ctttataact ttttttcatt
     7681 gttagttcct ctttcattcc tctctcaatt agctaagccg ttactggtac gccattctct
     7741 aaatttagta tcgctaatcc ttaatgctcg tcgaatatca atcattcgat aacctattgc
     7801 acgaagttct ttatatcttt tagcagttag cgtctctaac gtcatttcag gcaatgggtt
     7861 cgaatcaaaa atagaattcg aaccgattat ttgggccaat tgttgtactt gtaacagctc
     7921 ttctgaactt aattcagtga ttgctcgatt gcctatcatt gcatacattt tctgtcgaat
     7981 atacttcttt tcttgtttag acaacatttc tatccactcc tattttcatt atcatttgtt
     8041 acctccactt cagtaaatcc acaaataaat gaattcatat tccaagtacc attcccttca
     8101 actacaactt ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt taccaatatg
     8161 tttttcacta ctactaattt gtttgtatac cgtggaattg ctgttccgat atacttcact
     8221 ttatttccta cgctaattgt catttcctca ctcctattct tttttatcca aaactttttc
     8281 ctgtcctatt cctgccaacg aatatacttc actgcatgct taaacaaaac aaaatgttgc
     8341 cctgttttat ttccgtcttt tacttgttcc acatacagtt catatttggt cacacctttt
     8401 aaaacgccag atactttttt tcatccattg tcacaattag ttttttcatc aatcgattga
     8461 tactgcactg ctcatttttc ttgttcatat agtattcctt tctttttcaa aactagcctc
     8521 ttttgtttac tagttttcat caatcatcaa cgatttaaca gattttttct ttcgcagggt
     8581 ttctagcgtc agcaacccca aactctaaaa aagtgccttt gctcccttga aaaacgagcg
     8641 gacgattgta ctcgtcatca tatgtttttc atactacctg atccacgcaa ataacctttt
     8701 tttgttctgt gccacaccca tccatacgta tcgcttgaga ttcaccatac ttttttaggt
     8761 ggtttatcct tcctaagtcc ctgttagaca cgctgattaa cttagaaagt cctgtttgta
     8821 tccaattttc aaagatctcc caaaagaaat aagtaggttt acgtgtatcg cttctgttca
     8881 aagtacctgt ttgtacttga gtatttctac tcccctcgtt caagggattc cttccaccag
     8941 ctagcttttc ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg tgtccgtgtg
     9001 cttacttatt cttttttcga tagcgattat ccgctcgcta ctcgctattt tatactttgt
     9061 ttgttagctc aaataaaaac taacaaacaa aaaaggctag tttctttccg ttttattttt
     9121 ttaactgtcg ataaatagtt gttagtttaa ttccaagttc taaaaccaat acgcccaaca
     9181 aaaccacagg aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc atgtcttccc
     9241 tctttctttt tggtgtcgct gtggtcgttc ttgccgttca atacttcgat acatttttta
     9301 ttcattattt gctcgttttg ttttcctctg gactgctttt cttgcttttt gaatacttta
     9361 tacgcttttc gtgcctgaat taataggtct tgtcggcgtt tcattcgtga gaaaacaata
     9421 tttgaaacca tcaaatcctg gttcgaacaa ccacgattcg caataatttt cccatcaaaa
     9481 aaaatagttt tcacttgaga aaaaaacagt tggaaatctt gttttgttgc taattttttt
     9541 gtcaaaatca ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac aatccgattc
     9601 gataacttcc aacgttgccg attaaacaat gttacttcac aacaaatatg acgtaagtga
     9661 ttaggggtca ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa attttgggca
     9721 attgtgacag cccataaatc tgcgtttcca ggtcttcata actacaagaa cgcttggctt
     9781 gaagtaatga atattctgct tgatctgata gttttgttgc cattggatct agattcaatt
     9841 ctcttacaca acattcccaa aaattcaata gcgctctaaa ataaaatcct tttcgattct
     9901 gaattgcttt tccttcatga cgagcaattt tttcattttg aatacaccgt tgatacgttt
     9961 tgcttaaatc ttctgcaaat gtttccccta aaattcgata ctcgcttcca tcagataaac
    10021 catgtgtttg attatacttt cgttgccgct tttctataat tcgtttactg tcgtatatca
    10081 tgtttagctg ctctttcgct tcacgccatt cagctgaact gcgaacaatc catgtcaaaa
    10141 catctttcgg aacaaacgta ctttcgctgt aatgagcaat aaactctttt tgccaatcat
    10201 cagccatttc aacaggacta gtcgtatcga tttttgtatc tagattttct gtatctagat
    10261 tgttagaaga attattaaat aattgtgcag aattttcaca tggatttttt tcgtcaaaat
    10321 catcgaaaat cgctaaatta tcaacagatt cctcctcttc ttttccttgt gcagaatcta
    10381 cactggttga gtcaacagga cctaatgaat acacatcatt ttctgtcgta tctaattctc
    10441 ctagatacaa ccgagctttg tttctttttc ctgtttgttg atcaaatccc atatcctcta
    10501 tttctagtag ctcatattct ttcaattctt ttttgattgt attaacagaa cgtctgcaac
    10561 aattcaatgc attggctaat tcttgttccg taaattcaaa ataaacaaaa ccttgactat
    10621 ctacttgatt tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata tacgcgattt
    10681 ttgctttatc agacaatgag cgatattttt cacttgtaat aaataccttt ggaaattgat
    10741 agtataattc attgtattta cgagttgcgg taaatctttt tgttgccatc gttattacac
    10801 ctcatcttgt ccatatatta agaaagaatt ttggtgttgg tagacacgaa catcttccat
    10861 tgtagcaatc aattctttta gataaattcg accgaattca attgttagta agtcgtaatc
    10921 ttctaattca tttttgattt cttcaatttc ttcttcagac acattgagta aaatggctaa
    10981 ttcttgattg gtataagaaa tatagacatg tttcttttca tcaatccgtt tttcaattaa
    11041 ggattcttct aagtactcat tcagtaagct ataggcgtat tttgcttgtg cagtcatcgc
    11101 ataatatttt gacgatttta taaagaatag ttttggcata tgaaaagttg cttgtgtcag
    11161 cacatctgtt gcgcgaaatt tgttttttac catatttttt cctcctagaa aaacacgcaa
    11221 aaaaagctct gtataaatcg aattacacag agtttcttct tgttgatatt taatttctaa
    11281 aatacgtttc gggtaaaaac taaacactta ctaatattta gattgtagaa tcttcgcctt
    11341 catttattca ttttaaagag aaaaagatca agtgaaatgt gcccaaaagt ttgattctat
    11401 taatt
//
LOCUS       DADOOS010000087        11334 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.087, whole genome shotgun sequence.
ACCESSION   DADOOS010000087 DADOOS010000000
VERSION     DADOOS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.087"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(292..2301)
                     /gene="metG"
                     /locus_tag="J8C37_001930"
     CDS             complement(292..2301)
                     /gene="metG"
                     /locus_tag="J8C37_001930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAZ5305361.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2569..2847
                     /locus_tag="J8C37_001931"
     CDS             2569..2847
                     /locus_tag="J8C37_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305362.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2944..3765
                     /locus_tag="J8C37_001932"
     CDS             2944..3765
                     /locus_tag="J8C37_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAZ5305363.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4050..5060)
                     /locus_tag="J8C37_001933"
     CDS             complement(4050..5060)
                     /locus_tag="J8C37_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5305364.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5072..6025)
                     /locus_tag="J8C37_001934"
     CDS             complement(5072..6025)
                     /locus_tag="J8C37_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5305365.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6015..7085)
                     /locus_tag="J8C37_001935"
     CDS             complement(6015..7085)
                     /locus_tag="J8C37_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305366.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7091..8134)
                     /locus_tag="J8C37_001936"
     CDS             complement(7091..8134)
                     /locus_tag="J8C37_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5305367.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8135..9076)
                     /locus_tag="J8C37_001937"
     CDS             complement(8135..9076)
                     /locus_tag="J8C37_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5305368.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9160..9507
                     /locus_tag="J8C37_001938"
     CDS             9160..9507
                     /locus_tag="J8C37_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAZ5305369.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9577..11238)
                     /locus_tag="J8C37_001939"
     CDS             complement(9577..11238)
                     /locus_tag="J8C37_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5305370.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3192 a         2304 c         2063 g         3775 t
ORIGIN      
        1 ctttatgaat gtaaggacaa taaaaaagag taccagatga atcaattaaa atctgcccga
       61 ccctttatca ttatgctttt tttctgtttt tacgattcct attagtatta ttctaaatta
      121 acaaaaatca gcgaagctcg tacttctttt tctaggattt attttggata gttctctgtt
      181 tctgtattat atgattggct tcaagtaata agaaggaatt tacgaaaatt gcttctcaaa
      241 tttcggaaaa agggggtatg acacaactag tgtcataccc cctttgaatt tctatgcgat
      301 ttctgagcca tttggcatgg attttggtgc ttcgattact tgtaaagagc catctggtgc
      361 ttctgcagaa aggatcattc cttgactgat ttgtccacgc atctttcgtg gtttcagatt
      421 tgctacaatg acgacttttt tcccgatcaa ttcgcttgga tcaggataaa attcagcgat
      481 tcctgacaag atttgacggt cttggctgtc tccagcatct agtcggaatt gcagcaactt
      541 atctgcccct tctacttttt gacagttgat gacttctgct acttttagtt ctactttttc
      601 aaatacatca aatttgattt gtttttcttt cgttgaaaca agttctgttt cttctggatc
      661 ccatttgact gtatcttcgt ttgtttgagt accttcagac attttttctt gaataaaagc
      721 aacttcttct tccatatcta aacgtgggaa gattggtgta ccttttgcaa ctacttttgt
      781 tcctgaagga aattcgccaa agtgtaatcc ttcaagattc attgtttcag agtccaatcc
      841 tagttgattg aagatcttct ctggcgtttc tgtcatgatt ggctgaagta atatcgctgc
      901 gatacgcaaa gattcagcta aatggatcat gacactattc aattcagctt tcttttcttc
      961 gtcttttgct aatacccatg gttctgtttc atcaatgtat ttgtttgcac gagaaatcag
     1021 cacccagatt tcagccaatg ctgtattgaa ttccattttt tccattgcat catggtaacg
     1081 tccgacaacg ttagcagccg tcgtagacag ttcactatca aatggagtga caagtgaggc
     1141 atagtcagga acgagtccat cacaatattt gttgatcata gcaatcgttc gattcaataa
     1201 gttccctaga tcatttgcca gatcataatt gacacgggaa acaaaatctt ctggcgtaaa
     1261 gacaccatct gacccaaacg gaacagcacg aagcaaataa taacggagtg catctaaacc
     1321 ataacgttcc acaagcattt caggataaac gacatttcct tttgatttag acatttttcc
     1381 gtctttcatc aatagccagc catgcccaaa tactttttta ggtagaggca aatcaagggc
     1441 catcagcatg attggccaat agatcgtatg aaagcgaacg atttctttac cgaccatctg
     1501 aacatctgca ggccagtatt tttcgaatag actttcatct tcggagccat atcctagtgc
     1561 agtaatataa ttggataatg catcgatcca tacatacacc acatgtttcg gatcactctt
     1621 gactggtact ccccaagtaa aagttgttcg tgaaactgca agatcctcta atcctggttt
     1681 gataaagttg ttgatcattt cgtttttacg agattctggc tggatgaatt cgggatgttc
     1741 ttcatagtat tccaatagac ggtctgcgta tttgctcatg cggaagaagt atgattcttc
     1801 ttttaccaat tctacttcgt gtccacttgg cgctttacca ccgatcacgt taccttcgtc
     1861 atctttgtac acttctgcaa gttgtgtttc tgtaaagaat tcttcgtcag aaacagagta
     1921 ccagccttca tattcaccta agtaaatatc tccctgttct aataaacgat caaatatttt
     1981 ttgaacagct gctttatggt aatcatctgt cgtgcgaatg aatttatcat aactgatatc
     2041 aagcgttttc cataatttct tgacatcagc cgccatttta tctacatatt cttgcggttt
     2101 tacacctagt tcttctgctt ttgtttcgat tttttgacca tgttcatcca ctccagtgag
     2161 ataaaacaca tcaaagccca tcaaccgttt ataacgagca attgcgtcac atgcaatcgt
     2221 agtatacgag ttgccgatat gaagtttgcc gcttggataa tagattggcg tagtgatata
     2281 aaaagtttcc ttttctgcca cggggctctt cctcctttat cgcatgatgc gaattcgttt
     2341 ccttgctagt ataccataaa aaaagcatgc tgttagcttt ttttcaaaat gaaaaagact
     2401 ggataaacga cttctatcta cgttcaatct atcctttaaa aggatgaata ggttgaataa
     2461 atgcgggttt ttgttttgtt tcatatctac taaaaaacgg ctttttattt ctcccctcac
     2521 acgtggtata ataaagacta tctatcttag taagggactg gaacaatcat gcagaagaaa
     2581 catctttttt ttacacttag tattgctttt ctttcattag cacatttaat attcagctat
     2641 ttttatattc gtatgtatgg ttattttaac ctccatgggc atttgaacag ttttatgaca
     2701 gcagcttgga ttttgcgttt tatcattgac gtatatatcg ttatttgtgg cttctttgcc
     2761 attcgtgaag agcggtataa agtattgccg ttttatctgc ttttcttttt attcaatctc
     2821 atacttccat ttatttttca tatttaaatg ataacctaga tttttcaagt aaatccttgg
     2881 ttttttttca gagtctgcta caataaggta aaaagtactt ttactgtcaa tggaggtggt
     2941 cagatgaacc gttttgcttc taaagaagaa gaaaaattat attatgaaca taaagcaagt
     3001 gaagaagaat ttcttaactg gtatcaaaaa caggaacgtc cagaatatga caagccctct
     3061 ttgactgtcg atatcgtact gatgtgctat aacaaagaag aagatcagct gaaagttttg
     3121 ttgatccaaa gaaaagggca tccttatcga aattcatggg cattgcctgg tggctttgtc
     3181 caaaaggatg agtcaactgg agaaagcgtc ttaagagaaa caaaggaaga aactggtgtg
     3241 gttatttcaa aagaaaatat tgagcaactg catacattca gtacgcctaa tcgagatccg
     3301 cgaggctggg tcgtcacagt cagctactta gcctttattg gagaagaacc gctaatcgcc
     3361 ggagatgatg ccaaggaagt acgctggttc acactagaac gtcatagtaa caagatccat
     3421 ttatctagcg gagaagtggc tatctcgctt gatttagtca ctggagagtc ttctggcaaa
     3481 gatactttgg cttttgacca tagtcaaatc atcttgaaag cattcaaccg tgtagtcaat
     3541 aaaatggaac acgagccgca agttctacaa gttttaggta aagatttcac gattacggaa
     3601 gctcgaaaag tatttgctaa attcttaggg atcgattata aaacgatcga tcattccaat
     3661 tttaagaaag ctatgctcca atatttcgat gagatcggtg aacgtcctgt aggaatcggc
     3721 cgtccttcaa aaatctatca gctcaagccg gggcaccatg aataaggttg tgagcaaaag
     3781 attcagacct aatcattcaa gaaaaaacgc aaaaacaact ctttgtgagt tacgtttttt
     3841 gtttcagaat tttgatgtat tcgtaaactt tctgaataaa aaattaacaa tagcagaaaa
     3901 agctcgtacg aactttttct gctattgttt tattggtttg tcataattag tgagcttttc
     3961 ttcaaaagaa aaattcctag aaacaaaagt cattaagact ttttattaac aaggctaacc
     4021 ctaacgctcc tatcagtttc attcattttt tatcccttga taaccgaatg tctgcccatt
     4081 ttttctacta cttggctctt tcttacggaa ttgctttctt ctgctccgtc ggtattgatc
     4141 aaaagaaatt ttttattata ccattgggac ttataacgca attttgttat taatttatgc
     4201 cagtcgtgaa tagaaaaatc agcatgtgcc tcttcttttg gaaaagattc tacaaataag
     4261 tagtcatatt gtttgaccaa tttcccagat aattgttcaa gataatcttc tcgctggttc
     4321 ttttgtttca gataaagatc cattactttt ctcttttgtt tctgataatt gcttgcctga
     4381 tctaacctga tctttctatg gtgagcggag cggactttca cattcaattt ccgctgtgca
     4441 tgttgcagct tttcttctgt ctggtgaaat tttggtaaag ttacttctag aggtcgagat
     4501 gtttggatca atgtcttcgg atgataggcg atcccgatcc atttagctaa agattctttt
     4561 tttactggcg aaacatcaca aagtagtgcg acataaaatt cttcattata tcgagcagaa
     4621 atcgtagcgg agcgaatcga tccttcaact ggccgatgct gatttatctt gattagttct
     4681 ttttgttttg gtaactttaa gtagccattt tccagataga tcgtacgggt ttggttgttg
     4741 gtcgtataag attgccacat actttttttc gttttaagct ttgggaagct ggcacgtccc
     4801 ttgaaataat tgcgaaaagc ccgatccaaa ttaagctgtg catttgctaa agcaagactg
     4861 tctacttctt ttaagaatgg gtattctttt tttaatgtag cgggtgtgag cgaaaaattg
     4921 gctactcctg tttgttgcca acttttctgc cgctttttca gcaaatggtt ataggtaaat
     4981 cgagcacatc caaaagtacg aatcaattgc tgtttttgtt cttctgtcgg atacatacgg
     5041 aatttaaaag cttttagcat ctatgtccct cctagttagc aaccagaaga gattcctttt
     5101 gtcgttttag tttttttgcg taatatggga cttcctgctc agttgcatag acaaaatgtt
     5161 ctggtgcgat ttcacgaagt tgacgtactt gtccgtcatc tggttcaggt tgatatttga
     5221 ttcttctgcg ttttctctca tggtctggat ctggtagagg aatagcagat aacaaacttt
     5281 ctgtataagg atgaacaccg tgattataga tttcatcact tgttcccatc tctaataaca
     5341 agccgttatg cattacaccg attcggtcac taatatgttt aaccatcgac aaatcatgtg
     5401 caataaatag ataggtcaat tgatgttctt tttggagatc ttgcaacaaa ttgaccactt
     5461 gggcttggat cgacacatct agtgcagaaa ttggttcatc acaaatgata aaacgcggtt
     5521 ttactgccaa tgctcgtgca ataccaatac gctggcgttg cccacctgaa aattcatgag
     5581 gataacgagt accgtggctt ggattcaatc caacagttct caatagatca tcgacttttt
     5641 ttgcacgctc ttcagatgat ttagctagtc cattaacatc aattccttcg gcaatgatat
     5701 ctctgacttt catacgagga ttcaacgaag cgtatggatc ttgaaagatc atctgaacat
     5761 catgccggaa tttcgtcaaa ccttttttat ctttgatctt cgttatatct tgaccgtcga
     5821 ataatatctc accatcagta ggttcattca agcgaatgat cgtacggcca gtagtggatt
     5881 ttccgcttcc agattctcca actaaaccga atgtttctcc ttcataaatg tgaaagctga
     5941 tgtcatttac agcatgtact tcattctttt ttcctacatt gaaatactgt ttcaatcctt
     6001 taacttcaag tagtttattt ggcatcagca attcctcctt gaccgtaagt atgtttttgt
     6061 ttctctttaa agatagccca gcgacgttgg atttcttctg gcggtgtcac tttgggtgcc
     6121 tgcggagcaa gtagccaagt tgctgccttg tgtgtttttg atagttcaaa aaatggtggt
     6181 tcttgttctg catcgatttt catcgcgaat tcatttcttg gatagaatgc gtctcctttt
     6241 ggcgggttca ataaatccgg tggagaacca ggaatagcat acaatcgatc gttcgcacta
     6301 tctaatgtcg gcattgaacc aagcagtccc caagtatagg gatgttgcgg gttgtagaaa
     6361 atttcttccg atgttccaac ttcaacgatt cttcctccat acatgacagc gacacgatca
     6421 gcgacattcg ctactacacc aaggtcatga gtgataaaga taattgatgt atcgattttt
     6481 gtctgaattt ctttcagcag ctccaaaatc tgtgcttgaa tagtcacatc aagcgctgtt
     6541 gtaggttcat cagcaatcag gatttccgga taacatacca gtgcaatcgc gatcacgatc
     6601 cgttgacgct gacctcctga gaactgatgc ggataactgt tgatacgttt ctcagcttcg
     6661 ggtattccaa ctaatttaag caattctaat gcagcttttt gtccctcttt ttttgaaact
     6721 ttattatgct ttctaaggga ctcagcgact tgcttcccaa tgggcatcgt cggatctaaa
     6781 gaggtcattg gatcttggaa aatcatcgct atttctttac cacggatttt ttgcatctct
     6841 ctttctgatt ttccaacgat atcgttacct ttaaagagga tttgaccagc atctatattg
     6901 gcgttgcttg acagcaagcg catgatgctt ctggtcgtca ctgattttcc acttccagat
     6961 tccccaacga tcgccaatgt ttctccttta ttcaaatgaa aattgacacc gcgaatcgct
     7021 cgtacctttc cagcatacgt atcaaaagag atttctaagt ctttcacttc taaaattttc
     7081 gtcatcgtct tcactctttc attttaggat caaaggcatc acgcaatcca tcagcaagta
     7141 agttaaagcc aatcatgata accgataaag ttactgccgg aatccataat agataaggca
     7201 gatacaggaa tgtcttgtac ccttcgctca acatcgttcc taatgaagct gacggaggcg
     7261 taagacctaa accgataaag cttaaaaagg cttcgaagaa aatagcagat ggaatactaa
     7321 acatcatttg aataataatg acactagaga tattcggcaa aatatgtttg aatgcaattt
     7381 tcattcttga ttcacctaat gtcaatccag ctaaaacata ttcttgatct tttaatttca
     7441 ttgtttgtgc acgaacgata cgtgccattg gtatccagtt tgtgatggcc atagcagcca
     7501 cgattgaaaa aattcctggt tcaaaaacga ccaacatcaa aatcatgaca acgaggtttg
     7561 gaattcctga taagatttcc aaaaaacgtt gcatcacgtt atcgacacgt ccgcctaaca
     7621 aaccagaaac caacccgtaa gctacaccaa tcgttacatc tagtaatgca gcaataaatg
     7681 cgatcagcaa cgagattctc attcccataa ataagcggct taatacatca cgtcccaaac
     7741 cgtctgttcc taaaaagaaa tttacgtttg aagggacgtt tgcttgagca tatttatcaa
     7801 caagttcacc accaaccatt gcttttccat tcaatccatc gatattgatt cccggaatac
     7861 gaggaggcaa attgatataa gccacgtttt gggcagttgg atcatgagga gaaacaaaga
     7921 ttgtgatcac agagatcaaa atgatgacca atagtaaaac cattgaaata acggcagctt
     7981 tgtttttctt taaacggcgc catgaatctt ggataaaatt caaagaaggt gcagtaatct
     8041 gttcacgttc ttcttcggta tttttttgta atggaagaaa ttcatccgca gggatatctg
     8101 caactgtttc ataacgcttt ggtatcattt ccatttatcc tcggctccct tctgaaacac
     8161 gaattcttgg atcaactaga ccgtacaata gatccacgat caaaataaca aaaatcagca
     8221 taaatgagta aaggatcgtt acagccatga tcgtaggata atcatttgtt gtgattgact
     8281 taacgaattg ttccccgata ccaggaatag caaagatatt ttctaccacc agtgatccgg
     8341 tcatcaatgc tacagctaac ggtcctaata aagtcatcaa tggaatcaaa ctatttcgaa
     8401 gaccatgttt aaaggcgatt tcccaacggc tcaatccttt tgcttttgct aattccacat
     8461 aatcactatg cagtacttcc accatttccg tacggataaa ccgagcagag tcagcaagcg
     8521 gcgacatggc caacgcaatt gttggtaaga ttgtgtacac aaagccatcc cattttgcga
     8581 ttggcaaaac ttgtaatttg attgcaaaga tatattgtaa taaaacagca aatacaaagt
     8641 ttggaatgga acgacctaat atcgctacga gtgtagaaga tgtatctgcc catgtatttt
     8701 gcttcatggc agagatcgtt cctaaaatcg tccctaggac tgttccaaag ataattgcct
     8761 gtaaccctaa ctgcaaggat ggtccgattc ttccagctaa caaatgtgcg actggctggt
     8821 ttttaaattg gaaggaaatt ccaaaatctc cttgaagcaa attagaaaga taaatcccat
     8881 attgtacgat aactggttta tccaaaccga cttgtttatt tagcatctcg attgtttcag
     8941 gactcaaacg ttcctgattc gtatatggcg tgcccggcag caattgcatc aagaaaaagg
     9001 tgattgttgc aattaaccaa agcgtgatga tcatgaaaaa gacacgtttt aatacatatt
     9061 tgatataact attcactaat gtttccccct agcttttgac ttctgacaga tgtttcgcta
     9121 cattattaga taatgaacca aaaataaagg agcaatcaaa tgaaaaaaaa atctatcccc
     9181 tacgcagtag cttttctact gattctggtt attttaataa aaaatctcat caaccattcc
     9241 tttacactca ttcaattatc taatgatcta ttcttatggt cattgccgtt tctgattatc
     9301 ggcggttttt tatgggtctt ttcttcgggt ttcttcgatc attttcagcg atcggtccat
     9361 cttgcacgaa caaggaaccg aaagaaaaaa ccagaatttt cctcgctctc gtctgccagc
     9421 tacggtatgt acagcttttg gctgatcatt gccggaatac taattgctct ttctgctatt
     9481 ttcatgcttt tttctttgtt aggataagtg aatatgagaa cgaggttgcg acatttgccg
     9541 cagcctcgtt ttttctcatg ctcacggtat taacgattat tcttcaacgt atgtccattt
     9601 gtaatcccat gaagcgcctg ccgcatggtg aacgatacct ttgatttttt cattcactag
     9661 gtgaccttct gctttttggt acaccggaac gacacccata tcatcagaga tgatcttgat
     9721 tgctttttgg aaatcatccc aacgggcttg ttcatcgcca gcgtctgttg atgaagctgc
     9781 tttaactgct tcgtcatatt ctgcattgct ccagcgacca cggttgtatg aattacctgt
     9841 gacaaacaaa tcagtaaagc tgcttgggtc tgcatagtca gcagcccatc cgcctagtac
     9901 tgcgtcaaaa tcacccgaag ttgagcggtc aagacgtaca gagaatggaa caggtgttgg
     9961 tttgactgtt acgccatcca agttttcttg gattgagttt tgaacatatt cgattacttt
    10021 tttcgatgaa tcatcatcag aagccatcaa atcaaattct aaagaatcaa cgcccaattc
    10081 ttttttcgct ttttcccaat attcttttgc tttatctttg tcataagaaa ctaattttcc
    10141 agcttctttt gcaaagtctt cgttcgttgt tggacttttt accatatctg atgggatcaa
    10201 tcctgtagag acaactgatc cgtcacctag tacttgtttt actaaagctt cacgatcgat
    10261 agaataagaa atagcttttc ttaggttcac attgttgaat ggtgaattct tttcacgttg
    10321 attgaattcg atatagcttg tccgcgcttc tttaacagaa gtataagctg gatcgtttgc
    10381 gttttgctgt gctaactcac cagacaagat cacgtcatct gcttggccat ctttgaataa
    10441 gttcaatgca gtagaagatt cttttactac actgactttg atttcagaaa gttttactgt
    10501 atctttgtcc cagtaatgat catttttctt ataagtccat tctgtatctg ttcctggtcc
    10561 atcaaaatct tctaatgtga atggtccatt ataaacagct ttatcacttg cagatgcata
    10621 ggcatcccca ttttcttcca ctacggattg tttttgtggg aaaaatgatg ggaaagcaag
    10681 cagatattgg aaatatggtg tttgtttcgt caatttgatt tctaattcat agtcaccaac
    10741 tgctttgatc cctagttctg atttatcttt ctttccagct gtaatgtctt ctgcattttc
    10801 aactggcgca tacaggtaag catattcaga agctgtagct gggtctactg ttcgttgcca
    10861 gccataaacg taatccgctg ctgtaacagg atcaccattt gaccattttg cgtcttctcg
    10921 taatttgatt ttgtaagtta atccgtcttc actgacttct gccaactcgc tagctcctgc
    10981 cggctgtggt ttgctgtctt catcaagacg gtaaattcct tcataaacgt tattcaatgc
    11041 tgtaaaactg attgtatctg ttgcaactga taagtcagct gtaggcattt cttgaggcac
    11101 gactaagtta aatacgcctc ctccatctgc tgaaccgctt tttgctgaac tactgcttgt
    11161 ggctgaactg ccgccataac atccagcaag tacaaggccg cataccgcaa ccaaaccaaa
    11221 tgtaaatttc tttttcattt tacgtacccc tttattggaa ttttcagaat aattaatatt
    11281 ttctgaagtt gtttaagagt ttaatgaata attattaaaa aatcaacact tttt
//
LOCUS       DADOOS010000088        11258 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.088, whole genome shotgun sequence.
ACCESSION   DADOOS010000088 DADOOS010000000
VERSION     DADOOS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.088"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            6..305
                     /locus_tag="J8C37_001940"
     CDS             6..305
                     /locus_tag="J8C37_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305371.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="J8C37_001941"
     CDS             378..1739
                     /locus_tag="J8C37_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAZ5305372.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="J8C37_001942"
     CDS             1761..2021
                     /locus_tag="J8C37_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305373.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="J8C37_001943"
     CDS             2035..2787
                     /locus_tag="J8C37_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAZ5305374.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="J8C37_001944"
     CDS             complement(2881..3486)
                     /locus_tag="J8C37_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5305375.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="J8C37_001945"
     CDS             complement(3539..4513)
                     /locus_tag="J8C37_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAZ5305376.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="J8C37_001946"
     CDS             complement(4891..5670)
                     /locus_tag="J8C37_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305377.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="J8C37_001947"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="J8C37_001947"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAZ5305378.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="J8C37_001948"
     CDS             7161..8558
                     /locus_tag="J8C37_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5305379.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="J8C37_001949"
     CDS             8639..9079
                     /locus_tag="J8C37_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ5305380.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="J8C37_001950"
     CDS             9227..9553
                     /locus_tag="J8C37_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ5305381.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="J8C37_001951"
     CDS             9554..10303
                     /locus_tag="J8C37_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305382.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="J8C37_001952"
     CDS             complement(10383..10838)
                     /locus_tag="J8C37_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAZ5305383.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3575 a         1962 c         2305 g         3416 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagttt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagt
//
LOCUS       DADOOS010000089        11218 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.089, whole genome shotgun sequence.
ACCESSION   DADOOS010000089 DADOOS010000000
VERSION     DADOOS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.089"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(294..1142)
                     /locus_tag="J8C37_001953"
     CDS             complement(294..1142)
                     /locus_tag="J8C37_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAZ5305384.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1306..2208
                     /gene="gnd"
                     /locus_tag="J8C37_001954"
     CDS             1306..2208
                     /gene="gnd"
                     /locus_tag="J8C37_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAZ5305385.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2210..3211
                     /locus_tag="J8C37_001955"
     CDS             2210..3211
                     /locus_tag="J8C37_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAZ5305386.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3208..3492
                     /locus_tag="J8C37_001956"
     CDS             3208..3492
                     /locus_tag="J8C37_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ5305387.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3525..4322
                     /locus_tag="J8C37_001957"
     CDS             3525..4322
                     /locus_tag="J8C37_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ5305388.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4336..5169
                     /locus_tag="J8C37_001958"
     CDS             4336..5169
                     /locus_tag="J8C37_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5305389.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5187..6044
                     /locus_tag="J8C37_001959"
     CDS             5187..6044
                     /locus_tag="J8C37_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5305390.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6066..6575
                     /locus_tag="J8C37_001960"
     CDS             6066..6575
                     /locus_tag="J8C37_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305391.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6612..7013
                     /locus_tag="J8C37_001961"
     CDS             6612..7013
                     /locus_tag="J8C37_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305392.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7125..7478
                     /locus_tag="J8C37_001962"
     CDS             7125..7478
                     /locus_tag="J8C37_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305393.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7512..7955
                     /locus_tag="J8C37_001963"
     CDS             7512..7955
                     /locus_tag="J8C37_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ5305394.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7972..9057
                     /gene="uxuA"
                     /locus_tag="J8C37_001964"
     CDS             7972..9057
                     /gene="uxuA"
                     /locus_tag="J8C37_001964"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAZ5305395.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9050..9763
                     /locus_tag="J8C37_001965"
     CDS             9050..9763
                     /locus_tag="J8C37_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ5305396.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10515..10715
                     /locus_tag="J8C37_001966"
     CDS             10515..10715
                     /locus_tag="J8C37_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5305397.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3779 a         1750 c         2410 g         3279 t
ORIGIN      
        1 aggaaatact taattcacca aaattttttt taatttttta gaaaataaga aacaaaaata
       61 ataaaaaaaa gaaaagataa attttttact actgtatact aactttgtta tcgcatctaa
      121 caagtaactc gaataatacc aatacaacct tctccaaaga tgcgatgaca tacgttctaa
      181 cccaccgact attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata
      241 tttggtagtg gctttttttg ttttatggat ttttcgcttt ttctgttatg gtgctattct
      301 ttgatttcag aaatagtatg tcgaacttta gcgatagaac gacgtgattc tttttcgttg
      361 taaaacatca aaatcacata tagagagtcc aaaatgctta gttgagaaat acgggaagct
      421 aaagcttcag atcggaattc tgtttcttca gaaatagaaa taaaaacgac atcgccaagt
      481 ttagcaaggg aagaattagc ttggctagta ataacgattg tttttgctcc agtttttctt
      541 acggtttcag caatatggat tgtttctttt gattgccctg tgtgagaaat caaaacagca
      601 cagtcttttt ccgtcaataa agaagcttcc atcaattgaa tatggtaatc cgtgctgtga
      661 gaagttgcga taggggaacg caaaaattta tgataagcgt cagttgctac aatctctgaa
      721 ccgccaacac cgaaaaaata gacacgttta gattcattga taatagtagc cgcttttttt
      781 agatcctttt gttgtaacaa cttttttgtg tcatttaaag ttgaaatact agactcgaac
      841 accttttgcg ccatagtgag ctcagtatct gttttctgaa tattttcgtg gatagaaata
      901 gcagatagat cattttcttg gatcaacatg gccattttaa aatctttaaa tccattataa
      961 ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa
     1021 ttgctaatag gactatgact tactttgctt ggattttcta aaatataatc ggcgatagcc
     1081 tgttcttttg ggctgaagtc gttgtacatt gttttgattt gtagtcttat ggagacgctc
     1141 atgagaattc ctcattttca taataagatt atcatttttt ccgttctcat taatgttaaa
     1201 gatacaagga gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa
     1261 aaaatttttt tgtataatga gggtataaga aatggaggcg ctatcatgga aattggtgta
     1321 attggtttgg gaaaaatggg attgaattta gctttaaact taaaagagca ccatcacaaa
     1381 gtacaaggac tcgacatttc aagtacagct gttagtcagg caagagaaaa tgacattcct
     1441 gcttatcaag agatctctga atttctagct acttttgagc agaaaaaaat catctggtta
     1501 atgcttcctg ctggagagat aaccgaaaac atgttaagaa aactttttcc aaaactcgga
     1561 ccaggagaca ttattattga aggcggaaac tcttattata aagattcaat ccgtcgagca
     1621 gaagagtttc agaaaaatga aatcggttac ttggattgtg ggacttctgg aggaatcgaa
     1681 ggagcacgac atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg
     1741 gaacaattat tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga
     1801 agcggacact tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc
     1861 ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca
     1921 cgtgtttgga atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag
     1981 ttaaacgaac atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca
     2041 aaatggacgg ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt
     2101 ttgttcatga ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca
     2161 ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta
     2221 tcatatagca atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag
     2281 attaaagtca gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga
     2341 actagcggag ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg
     2401 acgagatttt tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta
     2461 caacaatatt gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc
     2521 tttagttgaa cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg
     2581 acgcacgaca caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt
     2641 cgtcggtagg acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag
     2701 cggagtaatc gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta
     2761 caaatcagat ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact
     2821 ggaacaatca gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat
     2881 ttctgataaa gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg
     2941 cgctctagtt gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata
     3001 tgccacagat gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga
     3061 acaagtagtg attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg
     3121 tgaaaaatgt gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt
     3181 ccagacaaag agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg
     3241 aaaacaggcc ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc
     3301 ttgaaatcat acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag
     3361 gcgctaccta aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat
     3421 tctggagaat gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa
     3481 tcgatttcgt agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca
     3541 atcttgcaag aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga
     3601 cacctcttgc tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt
     3661 acagacaagc tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat
     3721 atttcattgc cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag
     3781 aacaagattt tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct
     3841 gcaaagaaga gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac
     3901 tgacatcgga gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag
     3961 gattaggaga taaatacgca agaaatgtgg cagcacatgc tatgcatgac gctatcagta
     4021 aatatattcc ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct
     4081 atcaactggc actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg
     4141 aattagaact gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa
     4201 tagataaaat ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg
     4261 taacacagaa atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct
     4321 gaaggaggag gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg
     4381 gtagccgcgg ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct
     4441 cctttgatga ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt
     4501 gttacagccg ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca
     4561 gctgaaccat ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg
     4621 ccagaagcag cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag
     4681 tttcgttttt tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg
     4741 aatacaaaag ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta
     4801 ttcgttccgc ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc
     4861 aaagctttag agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg
     4921 gcaatcggta tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc
     4981 ttccttgctt attttatgag tgttgttttg aaatctttag atattacgat ggttacttat
     5041 gccatttttg ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta
     5101 cagcctgcca caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac
     5161 ggattttaat ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc
     5221 ctgaagaaat cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg
     5281 aaattcctca ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa
     5341 tcttgaaaaa actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac
     5401 tgttcttcaa cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta
     5461 tggaacagca aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact
     5521 tgatgtataa cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg
     5581 attcaggaac cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa
     5641 gtcctttagg cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg
     5701 tatttttcgc aagacaagct ttcactatgg gccgtaatgc tacaggccta aagcaaaaca
     5761 ctggtgttca gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc
     5821 ttgcgggaaa ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt
     5881 ttgttatcca ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa
     5941 tgggtgtgta cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc
     6001 tgactggaat attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga
     6061 ataagatggg tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa
     6121 tggtaacgtt atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag
     6181 caaaagataa atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa
     6241 caattgtcat tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat
     6301 acaattgtat tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa
     6361 tccccatgga agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt
     6421 tagtgacgaa atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg
     6481 aacaatatgg cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat
     6541 taaaagatgt attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag
     6601 agggcgaaaa aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata
     6661 tttaccgtaa gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag
     6721 atatttacag tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat
     6781 caaaaaatat ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg
     6841 atacacccgt tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa
     6901 aaacaatcta ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa
     6961 tgatgatata taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta
     7021 tcactgtact agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc
     7081 ctagcagaca aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc
     7141 gtacgattga tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa
     7201 agggcttaga cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact
     7261 ctgttggggc agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt
     7321 ggctgaaaca agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa
     7381 aaaagcaagc gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt
     7441 ggcaaaatgc caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag
     7501 tgagaagact aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga
     7561 aagatgcggc ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt
     7621 ctggggagga catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca
     7681 cagcagaacc catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc
     7741 tcttggcagt caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg
     7801 tgaatctgcc aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag
     7861 aagcggtgga acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag
     7921 cggaagaaaa aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg
     7981 ggatttagat ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata
     8041 ccgggaataa atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag
     8101 atttcagaaa ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga
     8161 attgaaagtg tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat
     8221 attgataact atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt
     8281 tatagcttca aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat
     8341 ggaagctatt ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg
     8401 tatcaattga tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg
     8461 ttgaaaaaat tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt
     8521 gaaaatctga cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa
     8581 atggctatcc atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa
     8641 aatcttggcg acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg
     8701 ctgtgcacag gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag
     8761 gtaggttcgc gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag
     8821 ttcaaagaaa cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa
     8881 gcgttggtgc aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg
     8941 ggtgaagtgg ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg
     9001 caaggtctat atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat
     9061 caagctcttc atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg
     9121 tgcttatgta aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt
     9181 actaactggc caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga
     9241 tgttccatat atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga
     9301 agataagaga gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag
     9361 aggaatccat acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa
     9421 aatggaaaat ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg
     9481 aaagccggaa ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga
     9541 agcacttgta tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt
     9601 gtcttttctc atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt
     9661 gaaagtagat gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca
     9721 tcaagcaaaa cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc
     9781 tagtcctctt tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt
     9841 ttattttgaa gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc
     9901 tactgttatc taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt
     9961 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
    10021 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
    10081 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
    10141 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
    10201 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
    10261 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
    10321 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
    10381 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
    10441 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
    10501 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
    10561 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
    10621 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
    10681 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
    10741 tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat
    10801 agtagtgtat aaaacaattt tgtggagcgt gaagctttta tggtagagga aaatacgaat
    10861 aaagtgatgt tgataaaaag attcctatct tgaatatgaa tcgagatgtc ttgaaaatga
    10921 atgagtttga ccaatcatta tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa
    10981 ttaatgaaaa agtaaaaaag aatatgttct aggaagaggg cggcacatct aaatttttaa
    11041 gtaacggaca gttcaaatat caagcattga aagaggctat cctactgttt gaaggaatac
    11101 gaatatataa aaacgacaaa gaggaaaaag tagttcatta aatcccttca agtgatgaaa
    11161 gaatgagatc taataaaacc tgatctttca tctgtttttt taataacgta ataatttt
//
LOCUS       DADOOS010000090        11211 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.090, whole genome shotgun sequence.
ACCESSION   DADOOS010000090 DADOOS010000000
VERSION     DADOOS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.090"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(25..144)
                     /locus_tag="J8C37_001967"
     CDS             complement(25..144)
                     /locus_tag="J8C37_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAZ5305398.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="J8C37_001968"
     CDS             complement(159..1343)
                     /locus_tag="J8C37_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAZ5305399.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="J8C37_001969"
     CDS             complement(1599..1853)
                     /locus_tag="J8C37_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305400.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="J8C37_001970"
     CDS             complement(1878..3020)
                     /locus_tag="J8C37_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAZ5305401.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="J8C37_001971"
     CDS             complement(3080..4432)
                     /locus_tag="J8C37_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAZ5305402.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="J8C37_001972"
     CDS             complement(4503..4847)
                     /locus_tag="J8C37_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAZ5305403.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="J8C37_001973"
     CDS             complement(4857..5231)
                     /locus_tag="J8C37_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ5305404.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="J8C37_001974"
     CDS             complement(5244..5558)
                     /locus_tag="J8C37_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ5305405.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="J8C37_001975"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="J8C37_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="J8C37_001976"
     CDS             complement(8976..9638)
                     /locus_tag="J8C37_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305406.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="J8C37_001977"
     CDS             10255..10485
                     /locus_tag="J8C37_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305407.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="J8C37_001978"
     CDS             10491..10790
                     /locus_tag="J8C37_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305408.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(10826..11197)
                     /locus_tag="J8C37_001979"
     CDS             complement(10826..11197)
                     /locus_tag="J8C37_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305409.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT         3116 a         2457 c         1879 g         3759 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgcttgaa attcattagg tgagtttgtt tattattttt tctgcttctc
    10861 gattcatcag cgccatatct gttttcgtaa tggtttcatt aggaatatag tcagcacatt
    10921 ttcttttctc taacattcta tacccactat tatgtattcc taaggtattc aattccttaa
    10981 ctgttgaaaa caaattccaa acggtttggt ggcttccttt tccgctactt ccattataaa
    11041 taatatcata attttctttc aaatactcat cagatttttt aaaagcactg tagtatgctc
    11101 ttgaaacagc agttcttcta taagattctt tagaattata tttatcatct gattcaattt
    11161 gtttggaaat agataaatat tcattaaagt caaacatttt attcccccaa a
//
LOCUS       DADOOS010000091        11151 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.091, whole genome shotgun sequence.
ACCESSION   DADOOS010000091 DADOOS010000000
VERSION     DADOOS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.091"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            243..512
                     /gene="rpsN"
                     /locus_tag="J8C37_001980"
     CDS             243..512
                     /gene="rpsN"
                     /locus_tag="J8C37_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAZ5305410.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            527..706
                     /gene="rpmF"
                     /locus_tag="J8C37_001981"
     CDS             527..706
                     /gene="rpmF"
                     /locus_tag="J8C37_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAZ5305411.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            738..1115
                     /locus_tag="J8C37_001982"
     CDS             738..1115
                     /locus_tag="J8C37_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305412.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1136..1285
                     /gene="rpmG"
                     /locus_tag="J8C37_001983"
     CDS             1136..1285
                     /gene="rpmG"
                     /locus_tag="J8C37_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAZ5305413.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1309..2292
                     /locus_tag="J8C37_001984"
     CDS             1309..2292
                     /locus_tag="J8C37_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAZ5305414.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2322..2444
                     /locus_tag="J8C37_001985"
     CDS             2322..2444
                     /locus_tag="J8C37_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAZ5305415.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2462..3985
                     /locus_tag="J8C37_001986"
     CDS             2462..3985
                     /locus_tag="J8C37_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAZ5305416.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4009..4248
                     /locus_tag="J8C37_001987"
     CDS             4009..4248
                     /locus_tag="J8C37_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305417.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4441..4902)
                     /locus_tag="J8C37_001988"
     CDS             complement(4441..4902)
                     /locus_tag="J8C37_001988"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5305418.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5097..5315)
                     /locus_tag="J8C37_001989"
     CDS             complement(5097..5315)
                     /locus_tag="J8C37_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5305419.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5433..7049
                     /locus_tag="J8C37_001990"
     CDS             5433..7049
                     /locus_tag="J8C37_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAZ5305420.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7290..8630)
                     /locus_tag="J8C37_001991"
                     /pseudo
     CDS             complement(7290..8630)
                     /locus_tag="J8C37_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8906..9061)
                     /locus_tag="J8C37_001992"
     CDS             complement(8906..9061)
                     /locus_tag="J8C37_001992"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAZ5305421.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9357..9689)
                     /locus_tag="J8C37_001993"
     CDS             complement(9357..9689)
                     /locus_tag="J8C37_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5305422.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10203..10394)
                     /locus_tag="J8C37_001994"
     CDS             complement(10203..10394)
                     /locus_tag="J8C37_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5305423.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10488..10874)
                     /locus_tag="J8C37_001995"
     CDS             complement(10488..10874)
                     /locus_tag="J8C37_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ5305424.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3701 a         1765 c         2151 g         3534 t
ORIGIN      
        1 ttgattggac taataaaagt gaattcttat ttttcagagc ctaaactgca tattctttta
       61 aatgatttga ttatctctat gtaccgataa attcgttttc aaatagttat ctattcatat
      121 gagttttatt tcatgttatt tattgttgac aagagcaact cttgttggta aagtatcagt
      181 gtcaaaacgt aacgattacg atttgaggtg tctaaatcaa aataaaagga gtataaaggt
      241 atatggcaaa aaagtcgaaa attgctaaag caaagaaaca aatggcgatg attgaaaagt
      301 atgcagataa acgtcaggaa ttaaaagccg caggagatcg caccgctctc gctaaattgc
      361 caagagattc aaatccgaat cgtctgaggc ttcgtgatca gacggatggt cgtccaagag
      421 ggtatatgcg aaagttcggt atgtcccgta ttaagtttag agaacttgcc catcaagggt
      481 tgattcctgg agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg cagtaccagc
      541 aagaaaaaca tctaaggcga agaaacggtt aagaagaact catcaaactg tagtaaaacc
      601 agaaatttca tttgacgaag cgaatggtga ttatagacgt agccatcatg tatctttaaa
      661 agggtactat aatggaaaga aagtaattaa aggagcttca aaatagttta atatataact
      721 tttaggaggg aatcaaaatg gaaattattt acccaccttt agttgaagaa ggattaaagt
      781 attatcttga aacgacccag caatcgctag ataaatgcac tttttatcgt tcgatggtag
      841 agagaggaat tattactgaa acgggtttac cgacccaaca ggcaattgaa aatggattag
      901 tcaaagatta ctatgaggac caaggcttat catttgatga ttttttaaga atctatccaa
      961 tttttgaaga gtatgacgag gagttatttc aatgtatcga tggttattgg gagatacctg
     1021 ttgatatgaa agaaaattta gtttcacaat tggaatctgg cgagttaact tttgaagatg
     1081 cacaacaaat tcaagcatat cttgaagatc gataagtatt gaaggaggaa gatttatgcg
     1141 tcaaaatatt attttggaat gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa
     1201 taaacggaat aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg
     1261 ggcaattttt aaagaagtga aatagctaag tggtaaaaag gtgacaaaat gggcatacca
     1321 gttacaatcg tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg
     1381 caagtctcac cctaccccag ggaagaaatt attataattg aaaatgagtt tggtgaagtt
     1441 gggattgatc acaaattatt agttcatagc gaggaacggg tgttacaact aaataatgga
     1501 tgtatgtgtt gcagtcttcg cagtgattta ttagcaatat tgatttcttt actggaaaca
     1561 atagaagaac atcaacaacc aatttcacaa atcatcattg aaactacagg tatagcagat
     1621 ccccaaccaa tcattcaaac acttttaaca gcgcctcaac taagaggaag agtatatatc
     1681 gatagtttat tgactgttat tgatagtgac agttttgaac ttattgaaag agaaccacaa
     1741 gcccttaagc aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat
     1801 ttatctgcac taaaaaaatt acagagagaa atcaaaagta ttaacccttt atccgatatt
     1861 cgaatgttca cacatagtga aacgattcaa aaaaaggatt tttataattt gaaaaaattt
     1921 aatcatccct tagtttttga aagtgattca tcagaaaatc actataatgg caaatactat
     1981 catcaacacc atcaagtaca tgagtttaaa tcaattctct taacttcaga tcaagacata
     2041 aaaaaagatt ttttactcca atggattgac tggctgattt taaccaacca agggagcctg
     2101 tatcgtttta aaggattgat tgctttacaa gagcaggatt ttatagttgc catgcaagag
     2161 gtaaacgaac aggttaattt tcaatacacc actcgacaaa aagaccaaac cactattatt
     2221 ctaatcggaa aaatgttgga tgaagaaagg atcaaacaga gttttgataa attactggcg
     2281 gaaagcaaat aatgataagt agtgattggg ggtaatttct aatggagaaa acggatatat
     2341 ctagtgcata tcgacgactt aaaagcccga atattaaaac tagaaaaagg gcattaaaaa
     2401 ttatcaaaga acataaacga aataaaatga agaaattagc ataattaagg aaggaaaaag
     2461 aatgaagaaa atcagtgtag gacttattgg gatagcagct ctaggattat tgggagcttg
     2521 ctccagcact aatgatgcaa aagtatcgaa tgataaagat ggaaaattag aaattgtaac
     2581 aacattctat ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga
     2641 tttaatggtt ccagcgggat cagaacctca tgactatgag ccctcagcta aggatatggc
     2701 aaaagctcat gatgctgatg tttttgtata tcataacgaa aacatggaat catgggtacc
     2761 aaaagctaaa gaaagttgga agaaagctgg gcctaatgta gtagaaggaa caaaagacat
     2821 gattttattg ccaggtagtg aagaagaaga tcatgatcac ggagaagaag atcaccatca
     2881 tgaactagat cctcatacgt gggtttcacc caaaatggca attaaggaag tttcaaatat
     2941 caaggatcag ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga
     3001 aaagtatttg actaaactga aacggttgga tgctgactat accactagtc ttaaagaagc
     3061 gaaacaaaaa agttttgtga cgcaacacgc agcctttgga tatttagctt tggattatgg
     3121 attgaaacaa gttcctattg ctggactaag tcctgaagaa gagccatctt ctggtcgatt
     3181 agctgaatta aaagagtatg ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa
     3241 tgctaatgat aaaattgcaa aaactttggc caacgaagct gggataaaat tggaagtact
     3301 gaatcctctt gaaagtctta caaaagaaca aatggataat ggtgaagatt atgtttcagt
     3361 aatggaagac aatctaaaag cgttagaaaa aacaactatg gttgctggtg aagaggtagt
     3421 tccagaaaaa gaagcgaagg atgaaaagac agttgctaat gggtacttta aagatgccga
     3481 tgtgaaggat cgcgaattga gtgattatac aggcgaatgg caatctgttt atccgttgtt
     3541 aaaagatgga attttggatg aagttttcga ttacaaagca aaaatcaata aagacatgac
     3601 agctgccgag tacaaagatt attatactac tggctataag acagatattg atactatcaa
     3661 cattaaagac aacaccattg attttgtagt caacggtgaa catcatcaat acacatacaa
     3721 atatgttgga tacaaaattt tgaattatga aaaaggaaac cgtggtgttc gttttaattt
     3781 tgaaactgat gatgcaggtg ctggtcgttt taaatatatt caatttagtg atcatggaat
     3841 tgcaccaagt aaggcagagc atttccatat tttctttggt ggagaaagtc aagaaaaact
     3901 ctataatgaa ttgcataatt ggcctacttt ctatccagct tcattgtcta aacatgaaat
     3961 tgctcaagaa atgatggctc attaaaattc aatttttgga ggaaatacat ggtaagaaaa
     4021 gtattattgt ctatggattc caagaatatt gatgcttatc ggcgttatcg atttgcaaag
     4081 caaatgagat taaatgggag tacattagct gatattgctg caactttaca tgttagtatt
     4141 gacacagttc atagagtatt aaaacgggat cctgattgtt gttttatcaa ggtcaatact
     4201 ggaagaagta gacgtttagc aaaacggcat aatagcttgg ccaattaatt gataatacca
     4261 gagtagatca tttttccctt atttggattt gatctgcttt ttattatgtc ttgaattgtc
     4321 aaatcaagcg caacacttta tcaaagaaga cttcataagg tgttctccaa tctaaacact
     4381 ttctaggtct gttattcagt ttttcaatca tctgatttat cgttgagtta tctaccgtag
     4441 ctatctcttt cgattttggc aagtattctc gaatgagtct attcgtattt taatttgttc
     4501 attgttgcca agggcatgag gatctggaaa ataaaagggc atattattca atgcttctgt
     4561 aactgcgaaa tgattggaga attcttttcc tctatctgga gtgatcgttt tgaccgcctc
     4621 atttggaata ctgcttaaaa gttcaatcat tttctcgctc acataagcgg ctttctttct
     4681 ggggaccttc tttcctgcta aaaggaagca tgattttctg tctgtcaaag tcactaaaca
     4741 agtaccacca gttttgccag ctacaatatc ggctttccaa tgaccaaaat cacttctatc
     4801 atctgcggat tttggacggc catggatcga atgagtaagg caaatctttc cacgagactc
     4861 gacatgattt tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt
     4921 attgccgtgt gagaggggtt ctgtatcgaa aagatcattg caaattgcac gttagatcgt
     4981 gataaaacta attgaaaagt ccatatgttc atgcttcaaa cgattcgaga tttcctctgg
     5041 cgaccactga aaatcgacaa ataaatgacg aacaattgac caaactcacg gatcatttag
     5101 aatgtgataa cgtccgcatt tttttcttcg ataagcataa tttttttccg ctttggaagg
     5161 tgaatagttt ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga
     5221 atgtcgttgc caattatact aatagaaaat ccttcttcgt gaagcagaaa gattctttct
     5281 cgttcgagta tagtaagatg acgatactgg ctcataatta ttttccttct aggagtgttc
     5341 tcgacaaact tatactagtt ggatatcgat tatgactcat ttttatgtgt tgcacttaaa
     5401 gtgtaaattc aaggtataga aagaggttcc ctatgaaaaa aatacctatt acaatcttaa
     5461 ctggctatct tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg
     5521 gaaaggaaat tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag
     5581 tcgacaagaa aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca
     5641 tttgttgtac attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg
     5701 acttgaatca aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag
     5761 caattatatt agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg
     5821 ccattattat agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga
     5881 ttcaggaaag aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt
     5941 taattgatca attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa
     6001 ccaagacgat ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa
     6061 ttaggacaat taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata
     6121 tagatgagac ttcctattca gtgggctggt tcaaagagtt agagcttggc tatcaaaatc
     6181 atattcctga aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc
     6241 ttgacgcaca aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag
     6301 caaaagggtt tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg
     6361 gtagctataa aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt
     6421 gtcaaatgat tatacaaaaa aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg
     6481 aacgcattaa taaactagtt tttattggta tgaagatgga tcaacaatgt attaaggaac
     6541 aacttgaggg ttgtttaact gattctctta atgcagataa tttccgaaaa atttcaccat
     6601 ttccatggaa aaagtctaat atgtttagcc agatatcgtt aatgaaattt ttaagaaaag
     6661 ctggtgtcaa gataggcgat gtcataatta ttggcatttt gattattacc tcttttattc
     6721 ccttatttgt attctctata gaaaaggaga atgaaacaac tgaagatgct atcaaatatg
     6781 ccatcgttcg aattgatggc aaggaaatgg atcgcttcga tttggataag atcaatcata
     6841 aactcgtgaa ttatcatcct gcgaagggac aatataatat tgttgagatt aaagatggaa
     6901 gaattcgagt taaagaagat aatagtcctg atcaaatcgc ggttagaact ggatggatct
     6961 caaaacccgg tcaaacgagt atttgtttac cccataagtt agttattagt attgagaaaa
     7021 aagaaagcac agattactac atttactaat ctggaaatag atgaggaggt ttttaataag
     7081 aaaaagatat tattattaat aatagcaggg gctttattaa gtgtgttaag cgcttgtgga
     7141 caagaaaaag aagcggtgac aacaggcaca agtatcttta acgggacctg caatggttta
     7201 gttaacagtc ggttagatta cttattttgg ctcttcgtca atgagtgttg gtgaagaaat
     7261 tcaatagaat aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt
     7321 gttctggctt tagtcagttg ggtagtattc ttagttttct taaaatattt tcctcgatat
     7381 aaaaagattc gttctcggta gttttgaaag tttctatagc cataggcaat tcgttttagt
     7441 actttgatat ggttattcaa acattctata gggccgttgg aatagggaac gcgcaacgct
     7501 cgtttgattt ctgtttggta tttcttgaag gtcgtgaaca ctggttggta gtgctctggc
     7561 aactccttgt agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata
     7621 acggaaagaa aatcctggta gacttcgtag cctcgcttta aggaatcatc ataagataat
     7681 agccgatcga ctatctctgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga
     7741 tgctgacctt tcaattccca cgcattcttt tgaagtagtt tccagtattt ctttaagtgc
     7801 ttcgaccgac gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaaa
     7861 gcccgaccaa tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa
     7921 cactctttca ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgcacttgt
     7981 tcacggactt ttatagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt
     8041 tccacaatat caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc
     8101 gagacatttt tcactgattt gaattcatcg aaacacacca cttctggtaa cagctggctc
     8161 aaagaaatgg ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg
     8221 tgcttcatac gtgcgatcaa agacatagaa agaggttcgc ttaatagctc aagaatggct
     8281 tgtttgactc tgcgagcaat ggagcagcga cgatcactaa ctgaatcttc tgctaaaaaa
     8341 gtctttccac agtgtttaca tttgaagcgc tgtttgttaa tccgcagaat ggtcttgtag
     8401 ttactgacat cattcaataa gatggatact tttttccagc cctatttaat gatcgtttgt
     8461 tcattttgat agtggcgatg aaagcattcc tccggttgat aggtcaatgt gccatcaaaa
     8521 acaaaacatg tttcgtcatt gattcgctct ttgcgaaagg catcttcatg aaagataaga
     8581 tttaggtcta gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac
     8641 tcctttgaat ggttttcgtc gactttattc taaaggattt taaggtgtct gtttgcgttt
     8701 attagaaaaa agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat
     8761 cccttatttt cttcgcttca aattgtcaag gagtcatcat aaattttgta tggattcttt
     8821 tgtttgggta gtagtcttct aaataatctt ggattaacac cttcatctct attttacttt
     8881 tatatttgtt ggagtcgatt aattctcatt tgattgatcg atgaaacgct tcaattactg
     8941 cattgtcaac aggagtccca gtttggctca ctgaatgtgt gatgtggtgg tcgttcaaca
     9001 gctcttgata aataaaagac cgatgctggc tacctctatc ggaatgaatt accgtaggca
     9061 attctcctta ataaatttgt aatggacgga cgacaatctc ttttttcatg tgtgaatcta
     9121 ccatatagta acgtagaata cgagttgcta agtcaatata ggtggacaaa tagaggcgac
     9181 cgtcattaca tggaatgtaa gtaatatcca tcacccaaac agcatcaatt tggttctgtt
     9241 caaattatcg attcaacatt tttttaccat ttctttgatt gttttttctc taccatgaga
     9301 agatttgcgt cgatgaaatc ctcttgtgca taaattcatt tcacgcataa gaacagctac
     9361 aacacgttta ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc ctggagatcc
     9421 gtatcgatct ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt attttcctgt
     9481 tcaagaatgt agaaatctgc tttatcaggt aatctttttc cttgtccagc aaaagcattt
     9541 ttcccgtatc gacgatattc tgaaacccat cgggaaattg ttggacgaga gatattgtat
     9601 tcttcagcaa ttttcttggt agttactttt ttctcaagga tatcctgtga aatctttagt
     9661 ttaaatttac gatcatatgt ttttctcaat agagttcatt ccttccggta acgtaactga
     9721 tagtcaagaa aagtgtatca tagcccaact tagtatcagg gtttgtcaac taaactgtgg
     9781 aagttgatag ataagagttt ttaacaacca aaattctctt gactattttt gaaatcagac
     9841 aaattttgat tggacacata gttgaataaa tctaccgtcg ttacggaagg taaatcgcat
     9901 gcttttcaat tctagaggag aagtcctcgt tgaagtacca tagcccagtt ctggattcga
     9961 cctcctgccc acttcatacg taaataaagt aattttagga gtgagactgt gacaaaagtt
    10021 atttttaagc taaaaacgaa ttaaaaaacg ttgataatta aaatcattcg ttttagattc
    10081 tatgaatcat tatagaacgc tgtaaaatcg atactttata attataaaga agatagtttc
    10141 aaatttattg ttcgaaattt gaaactatct ttttacttat gtcacagact cccttagttg
    10201 agttattctt taatttcgaa tttcgagata gctagatata ttgcaagcat acattcttga
    10261 taacaatctt gatcatattt tccagttctc caggattgct tatgaatcaa agagctaaat
    10321 cttttgagaa ttgcctcaac agcttcttca tttccagcct ttgctaaaat gaaatcttct
    10381 tttaaagaat tcataaatct accttcttcc catggactta taatctattt ctttgggaag
    10441 gaggagaacg agcaaagaag catacaaaat tagttattat gcagatatta aaccagcatt
    10501 ttttgaattt ttttgtaaag tcgatgtttt aaatttgtcg caccctgtct tgtaatcccc
    10561 agaacctttc caatttcttt atcagtttta tcaaagataa atttttcgat aagaaaaatt
    10621 tgttcaggtt ctttcaacaa aggaaggatt tttgctaatt ttatatcttc aacaaggaga
    10681 gtcacattta aaacattaat cggttgttta aattttatag tatctttagt aaacaaatat
    10741 ggtgggagtt gatcaagttg ttcatgtttt tgaatttctt cttgtttttt ataataagta
    10801 ttagcggtgt tcaaaatgac cttttcaaag aatcgaatca actgggcatt tacgcgttcg
    10861 tcgttatctg acatattgca cacctcctcc attaagattt aaagggaaaa gaggcaaaaa
    10921 acacgaaaag aagctaatgc aattatgctg cgctgcgaat aacttctata agctcacatg
    10981 cattgatagg actgctgatg tttattttca aaaatgtttt tttagttgat aatggatagt
    11041 tatctttata cttagaaaag aaaattcata tatcttctta aatatcaaac atgtccttat
    11101 ggctaagaaa atgtcttgag ctttttacgt ataataaatc ttttctaaaa c
//
LOCUS       DADOOS010000092        10948 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.092, whole genome shotgun sequence.
ACCESSION   DADOOS010000092 DADOOS010000000
VERSION     DADOOS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.092"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            81..1340
                     /gene="lysA"
                     /locus_tag="J8C37_001996"
     CDS             81..1340
                     /gene="lysA"
                     /locus_tag="J8C37_001996"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAZ5305425.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1327..2127
                     /gene="dapF"
                     /locus_tag="J8C37_001997"
     CDS             1327..2127
                     /gene="dapF"
                     /locus_tag="J8C37_001997"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAZ5305426.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2130..3011
                     /locus_tag="J8C37_001998"
     CDS             2130..3011
                     /locus_tag="J8C37_001998"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAZ5305427.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3008..3724
                     /locus_tag="J8C37_001999"
     CDS             3008..3724
                     /locus_tag="J8C37_001999"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAZ5305428.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3730..4425
                     /gene="dapD"
                     /locus_tag="J8C37_002000"
     CDS             3730..4425
                     /gene="dapD"
                     /locus_tag="J8C37_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAZ5305429.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4441..5442
                     /locus_tag="J8C37_002001"
     CDS             4441..5442
                     /locus_tag="J8C37_002001"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAZ5305430.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5439..6800
                     /locus_tag="J8C37_002002"
     CDS             5439..6800
                     /locus_tag="J8C37_002002"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAZ5305431.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6760..7911
                     /locus_tag="J8C37_002003"
     CDS             6760..7911
                     /locus_tag="J8C37_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAZ5305432.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7886..9055
                     /locus_tag="J8C37_002004"
     CDS             7886..9055
                     /locus_tag="J8C37_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ5305433.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9230..10609
                     /locus_tag="J8C37_002005"
     CDS             9230..10609
                     /locus_tag="J8C37_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ5305434.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3635 a         1915 c         2456 g         2942 t
ORIGIN      
        1 tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa
       61 gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat
      121 tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga
      181 tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct
      241 ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag
      301 atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta
      361 tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt
      421 tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac
      481 gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc
      541 atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag
      601 agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc
      661 atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat
      721 tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg
      781 ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga
      841 ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg
      901 aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca
      961 aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc
     1021 cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa
     1081 cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag
     1141 cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg
     1201 gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata
     1261 atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag
     1321 tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt attagattac
     1381 gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat
     1441 acagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat
     1501 ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat
     1561 cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt
     1621 acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct
     1681 ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat
     1741 ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcatacagt tgtttttgtt
     1801 gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg
     1861 ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc
     1921 cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct
     1981 tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa
     2041 caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat
     2101 gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca
     2161 cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc
     2221 atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc
     2281 tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg
     2341 tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc
     2401 attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa
     2461 gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg attccgctta
     2521 ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt
     2581 tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga
     2641 tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga
     2701 tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac
     2761 ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag
     2821 aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc
     2881 ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag
     2941 tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg
     3001 ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt
     3061 tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac
     3121 gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga
     3181 gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta
     3241 tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag
     3301 tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat
     3361 gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc
     3421 atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt
     3481 atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc
     3541 aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat
     3601 caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg
     3661 gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt
     3721 gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg
     3781 cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt
     3841 ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa
     3901 gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt
     3961 cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat
     4021 caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca
     4081 gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg
     4141 ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg
     4201 gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa
     4261 ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg
     4321 gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag
     4381 acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa
     4441 atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc
     4501 aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt
     4561 tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa
     4621 acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag
     4681 tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg
     4741 gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat
     4801 cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta
     4861 caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc
     4921 gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat
     4981 ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat
     5041 acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg
     5101 ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca
     5161 gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca
     5221 gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga
     5281 ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg
     5341 ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt
     5401 gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag
     5461 cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg
     5521 ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag
     5581 atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg
     5641 attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg
     5701 ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa
     5761 agataactga agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg
     5821 agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga
     5881 aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa
     5941 tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg
     6001 ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga
     6061 attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa
     6121 tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc
     6181 ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata
     6241 ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg
     6301 gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata
     6361 tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat
     6421 caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga
     6481 ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa
     6541 tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga
     6601 ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct
     6661 tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa
     6721 cagtagtgaa gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg
     6781 gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat
     6841 gaacaagtaa aaaacagcgg ggccgttatc catgaaatcg gcgaaacagg actactcctt
     6901 tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca
     6961 gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt
     7021 ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac taaacacatt
     7081 gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc
     7141 gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc
     7201 tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg
     7261 gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt
     7321 gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt
     7381 gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga
     7441 acgatccgca atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt
     7501 tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct
     7561 acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc
     7621 ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa
     7681 gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc
     7741 ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat
     7801 ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc
     7861 agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct
     7921 ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga
     7981 tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc
     8041 aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga
     8101 actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca
     8161 aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt
     8221 tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt
     8281 agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg
     8341 tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat
     8401 tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat
     8461 cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt
     8521 gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat
     8581 aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca
     8641 aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac
     8701 gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat
     8761 gaaagagcaa tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt
     8821 tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca
     8881 aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc
     8941 aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat
     9001 ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat
     9061 agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt
     9121 ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt
     9181 ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat
     9241 caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac
     9301 acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat
     9361 gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa
     9421 tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa
     9481 aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa
     9541 agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt
     9601 accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat
     9661 caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat
     9721 tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt
     9781 gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca
     9841 cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa
     9901 cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca
     9961 agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg
    10021 tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg
    10081 agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac
    10141 tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa
    10201 accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc
    10261 aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta
    10321 tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat
    10381 ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta
    10441 tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga
    10501 agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt
    10561 gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat
    10621 atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa
    10681 aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac
    10741 accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag
    10801 accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt
    10861 aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag
    10921 atcaagagat tgaagcggaa atgcttaa
//
LOCUS       DADOOS010000093        10928 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.093, whole genome shotgun sequence.
ACCESSION   DADOOS010000093 DADOOS010000000
VERSION     DADOOS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.093"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..287)
                     /locus_tag="J8C37_002006"
     CDS             complement(<1..287)
                     /locus_tag="J8C37_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305435.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="J8C37_002007"
     CDS             429..1547
                     /locus_tag="J8C37_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ5305436.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="J8C37_002008"
     CDS             1675..3051
                     /locus_tag="J8C37_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAZ5305437.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="J8C37_002009"
     CDS             3452..5032
                     /locus_tag="J8C37_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAZ5305438.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="J8C37_002010"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="J8C37_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="J8C37_002011"
     CDS             complement(5658..5978)
                     /locus_tag="J8C37_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAZ5305439.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="J8C37_002012"
     CDS             complement(6126..8360)
                     /locus_tag="J8C37_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAZ5305440.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="J8C37_002013"
     CDS             8839..9105
                     /locus_tag="J8C37_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAZ5305441.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="J8C37_002014"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="J8C37_002014"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAZ5305442.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DADOOS010000094        10685 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.094, whole genome shotgun sequence.
ACCESSION   DADOOS010000094 DADOOS010000000
VERSION     DADOOS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.094"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            23..646
                     /locus_tag="J8C37_002015"
     CDS             23..646
                     /locus_tag="J8C37_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305443.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="J8C37_002016"
     CDS             1096..2145
                     /locus_tag="J8C37_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5305444.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="J8C37_002017"
     CDS             2142..3023
                     /locus_tag="J8C37_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5305445.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="J8C37_002018"
     CDS             3026..3796
                     /locus_tag="J8C37_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5305446.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="J8C37_002019"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="J8C37_002019"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAZ5305447.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="J8C37_002020"
     CDS             5107..6270
                     /locus_tag="J8C37_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAZ5305448.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="J8C37_002021"
     CDS             6387..6797
                     /locus_tag="J8C37_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAZ5305449.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="J8C37_002022"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="J8C37_002022"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAZ5305450.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="J8C37_002023"
     CDS             9337..9654
                     /locus_tag="J8C37_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305451.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="J8C37_002024"
     CDS             9814..10050
                     /locus_tag="J8C37_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAZ5305452.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="J8C37_002025"
     CDS             10047..10445
                     /locus_tag="J8C37_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAZ5305453.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3732 a         1775 c         2256 g         2922 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagat
//
LOCUS       DADOOS010000095        10599 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.095, whole genome shotgun sequence.
ACCESSION   DADOOS010000095 DADOOS010000000
VERSION     DADOOS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.095"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(27..725)
                     /locus_tag="J8C37_002026"
     CDS             complement(27..725)
                     /locus_tag="J8C37_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAZ5305454.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="J8C37_002027"
     CDS             complement(718..2070)
                     /locus_tag="J8C37_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAZ5305455.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="J8C37_002028"
     CDS             complement(2293..3693)
                     /locus_tag="J8C37_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAZ5305456.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="J8C37_002029"
     CDS             3854..4591
                     /locus_tag="J8C37_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAZ5305457.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="J8C37_002030"
     CDS             4716..5195
                     /locus_tag="J8C37_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAZ5305458.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="J8C37_002031"
     CDS             5192..5959
                     /locus_tag="J8C37_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305459.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="J8C37_002032"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="J8C37_002032"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ5305460.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="J8C37_002033"
     CDS             complement(6862..7464)
                     /locus_tag="J8C37_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ5305461.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="J8C37_002034"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="J8C37_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="J8C37_002035"
     CDS             8145..8729
                     /locus_tag="J8C37_002035"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAZ5305462.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="J8C37_002036"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="J8C37_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAZ5305463.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..>10599
                     /gene="prmC"
                     /locus_tag="J8C37_002037"
     CDS             9798..>10599
                     /gene="prmC"
                     /locus_tag="J8C37_002037"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAZ5305464.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKD"
BASE COUNT         3638 a         1908 c         2088 g         2965 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatc
//
LOCUS       DADOOS010000096        10504 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.096, whole genome shotgun sequence.
ACCESSION   DADOOS010000096 DADOOS010000000
VERSION     DADOOS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.096"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(219..704)
                     /locus_tag="J8C37_002038"
     CDS             complement(219..704)
                     /locus_tag="J8C37_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAZ5305465.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1184..1825
                     /locus_tag="J8C37_002039"
     CDS             1184..1825
                     /locus_tag="J8C37_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAZ5305466.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1844..2479
                     /locus_tag="J8C37_002040"
     CDS             1844..2479
                     /locus_tag="J8C37_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305467.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2497..3312
                     /locus_tag="J8C37_002041"
     CDS             2497..3312
                     /locus_tag="J8C37_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAZ5305468.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3631..5067
                     /locus_tag="J8C37_002042"
     CDS             3631..5067
                     /locus_tag="J8C37_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5305469.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5256..7703
                     /locus_tag="J8C37_002043"
     CDS             5256..7703
                     /locus_tag="J8C37_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAZ5305470.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7933..9000
                     /gene="rlmN"
                     /locus_tag="J8C37_002044"
     CDS             7933..9000
                     /gene="rlmN"
                     /locus_tag="J8C37_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAZ5305471.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9193..10296)
                     /locus_tag="J8C37_002045"
     CDS             complement(9193..10296)
                     /locus_tag="J8C37_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAZ5305472.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3469 a         1797 c         2283 g         2955 t
ORIGIN      
        1 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
       61 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      121 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      181 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      241 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      301 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      361 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      421 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      481 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      541 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      601 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      661 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      721 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      781 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      841 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      901 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      961 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1021 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1081 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1141 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1201 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1261 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1321 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1381 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1441 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1501 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1561 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1621 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1681 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1741 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1801 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1861 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1921 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1981 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2041 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2101 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2161 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2221 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2281 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2341 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2401 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2461 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2521 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2581 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2641 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2701 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2761 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2821 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2881 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2941 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3001 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3061 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3121 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3181 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3241 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3301 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3361 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3421 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3481 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3541 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3601 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3661 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3721 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3781 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3841 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3901 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3961 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     4021 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4081 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4141 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4201 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4261 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4321 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4381 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4441 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4501 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4561 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4621 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4681 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4741 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4801 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4861 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4921 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4981 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     5041 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5101 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5161 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5221 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5281 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5341 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5401 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5461 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5521 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5581 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5641 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5701 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5761 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5821 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5881 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5941 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     6001 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6061 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6121 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6181 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6241 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6301 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6361 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6421 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6481 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6541 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6601 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6661 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6721 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6781 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6841 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6901 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6961 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     7021 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7081 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7141 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7201 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7261 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7321 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7381 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7441 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7501 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7561 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7621 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7681 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7741 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7801 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7861 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7921 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7981 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     8041 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8101 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8161 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8221 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8281 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8341 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8401 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8461 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8521 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8581 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8641 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8701 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8761 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8821 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8881 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8941 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     9001 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9061 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9121 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9181 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9241 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9301 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9361 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9421 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9481 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9541 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9601 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9661 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9721 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9781 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9841 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9901 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9961 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    10021 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10081 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10141 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10201 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10261 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10321 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10381 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10441 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    10501 tctt
//
LOCUS       DADOOS010000097        10500 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.097, whole genome shotgun sequence.
ACCESSION   DADOOS010000097 DADOOS010000000
VERSION     DADOOS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.097"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(210..962)
                     /gene="xth"
                     /locus_tag="J8C37_002046"
     CDS             complement(210..962)
                     /gene="xth"
                     /locus_tag="J8C37_002046"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAZ5305473.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1075..1995
                     /locus_tag="J8C37_002047"
     CDS             1075..1995
                     /locus_tag="J8C37_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ5305474.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2068..3003
                     /gene="murB"
                     /locus_tag="J8C37_002048"
     CDS             2068..3003
                     /gene="murB"
                     /locus_tag="J8C37_002048"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAZ5305475.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3301..4779
                     /locus_tag="J8C37_002049"
     CDS             3301..4779
                     /locus_tag="J8C37_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ5305476.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5056..7116
                     /locus_tag="J8C37_002050"
     CDS             5056..7116
                     /locus_tag="J8C37_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305477.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7199..7768
                     /locus_tag="J8C37_002051"
     CDS             7199..7768
                     /locus_tag="J8C37_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305478.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7943..8749)
                     /locus_tag="J8C37_002052"
     CDS             complement(7943..8749)
                     /locus_tag="J8C37_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305479.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8887..10401
                     /locus_tag="J8C37_002053"
     CDS             8887..10401
                     /locus_tag="J8C37_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAZ5305480.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3723 a         1750 c         2070 g         2957 t
ORIGIN      
        1 ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
       61 gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc agtttctcgg
      121 tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt tcttaatact
      181 cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac tggacaatga
      241 tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc taaatctttg
      301 ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt gaatcggtag
      361 ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt atcgataaac
      421 ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc agcatttttt
      481 tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag atccccacac
      541 acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc ttcttcccat
      601 tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta acaggttaca
      661 agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg gatatcgata
      721 cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt gccggaatag
      781 ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa atcaatttgt
      841 cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc atgaaaaact
      901 tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga gataaatttc
      961 aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt gtacctcatt
     1021 ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa gtagatgaag
     1081 attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac ttatgcgaaa
     1141 aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa agaaagaatt
     1201 caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt agaagaaaaa
     1261 atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc caaaaaatgt
     1321 ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct aaatgaagtt
     1381 cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg tttcaaccgg
     1441 cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt gatccacctg
     1501 caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga tttgttttta
     1561 catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac aaaataccag
     1621 ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac acaaaatcaa
     1681 acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata tcaagtaaca
     1741 agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca agaggctgat
     1801 ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg cgggtttgag
     1861 ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac agaattgctt
     1921 aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa agatcactac
     1981 cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa taatgaggac
     2041 gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga aagcttagcc
     2101 acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa aacaggtgga
     2161 ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat catcgattat
     2221 tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct gatcgttcaa
     2281 gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat tcatgtcaaa
     2341 ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta tgcagctttg
     2401 gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt cggtggagcg
     2461 gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc tgaagtggat
     2521 cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt attttcttat
     2581 cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt cagtttgcaa
     2641 acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt gcgtcaatca
     2701 aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt aggacactat
     2761 acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg tgcacaagtc
     2821 tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac agattatatt
     2881 gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc tttagaaaca
     2941 gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag tttagccaat
     3001 taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc ctgctctttt
     3061 ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa
     3121 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
     3181 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
     3241 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
     3301 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
     3361 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
     3421 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
     3481 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
     3541 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
     3601 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
     3661 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
     3721 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
     3781 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
     3841 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
     3901 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
     3961 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
     4021 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
     4081 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
     4141 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
     4201 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
     4261 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
     4321 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
     4381 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
     4441 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
     4501 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
     4561 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
     4621 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
     4681 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
     4741 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
     4801 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
     4861 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
     4921 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
     4981 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
     5041 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
     5101 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
     5161 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
     5221 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
     5281 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
     5341 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
     5401 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
     5461 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
     5521 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
     5581 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
     5641 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     5701 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     5761 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     5821 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     5881 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     5941 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     6001 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     6061 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     6121 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     6181 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     6241 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     6301 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     6361 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     6421 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     6481 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     6541 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     6601 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acgagaagaa
     6661 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     6721 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     6781 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     6841 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     6901 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     6961 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     7021 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     7081 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     7141 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     7201 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     7261 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     7321 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     7381 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     7441 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     7501 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     7561 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     7621 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     7681 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     7741 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     7801 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     7861 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     7921 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     7981 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     8041 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     8101 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     8161 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     8221 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     8281 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     8341 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     8401 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     8461 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     8521 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     8581 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     8641 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     8701 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     8761 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     8821 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     8881 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     8941 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     9001 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     9061 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     9121 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     9181 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     9241 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     9301 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     9361 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     9421 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     9481 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     9541 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     9601 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     9661 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     9721 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     9781 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     9841 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     9901 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     9961 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    10021 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    10081 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    10141 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    10201 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    10261 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    10321 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    10381 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    10441 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
//
LOCUS       DADOOS010000098        10115 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.098, whole genome shotgun sequence.
ACCESSION   DADOOS010000098 DADOOS010000000
VERSION     DADOOS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.098"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            119..625
                     /locus_tag="J8C37_002054"
     CDS             119..625
                     /locus_tag="J8C37_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAZ5305481.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="J8C37_002055"
     CDS             642..1541
                     /locus_tag="J8C37_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAZ5305482.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="J8C37_002056"
     CDS             1628..2512
                     /locus_tag="J8C37_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAZ5305483.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="J8C37_002057"
     tRNA            complement(2704..2775)
                     /locus_tag="J8C37_002057"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="J8C37_002058"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="J8C37_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAZ5305484.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4372..5409
                     /locus_tag="J8C37_002059"
     CDS             4372..5409
                     /locus_tag="J8C37_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAZ5305485.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="J8C37_002060"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="J8C37_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAZ5305486.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="J8C37_002061"
     CDS             6561..7751
                     /locus_tag="J8C37_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAZ5305487.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="J8C37_002062"
     CDS             7855..8610
                     /locus_tag="J8C37_002062"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAZ5305488.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="J8C37_002063"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="J8C37_002063"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAZ5305489.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3541 a         1871 c         2095 g         2608 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaat
//
LOCUS       DADOOS010000099        10103 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.099, whole genome shotgun sequence.
ACCESSION   DADOOS010000099 DADOOS010000000
VERSION     DADOOS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.099"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..832
                     /locus_tag="J8C37_002064"
     CDS             <1..832
                     /locus_tag="J8C37_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAZ5305490.1"
                     /translation="DIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPNV
                     KKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALANL
                     HQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETDRWNVQRQEK
                     YLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAMS
                     QLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLENF
                     "
     gene            848..1585
                     /locus_tag="J8C37_002065"
     CDS             848..1585
                     /locus_tag="J8C37_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAZ5305491.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1740..2666)
                     /locus_tag="J8C37_002066"
     CDS             complement(1740..2666)
                     /locus_tag="J8C37_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAZ5305492.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            2941..3774
                     /locus_tag="J8C37_002067"
     CDS             2941..3774
                     /locus_tag="J8C37_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAZ5305493.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3808..4647
                     /locus_tag="J8C37_002068"
     CDS             3808..4647
                     /locus_tag="J8C37_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5305494.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4673..5233
                     /locus_tag="J8C37_002069"
     CDS             4673..5233
                     /locus_tag="J8C37_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ5305495.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5432..7153
                     /locus_tag="J8C37_002070"
     CDS             5432..7153
                     /locus_tag="J8C37_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAZ5305496.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7318..8460
                     /locus_tag="J8C37_002071"
     CDS             7318..8460
                     /locus_tag="J8C37_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAZ5305497.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8476..9687
                     /gene="thiI"
                     /locus_tag="J8C37_002072"
     CDS             8476..9687
                     /gene="thiI"
                     /locus_tag="J8C37_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAZ5305498.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3456 a         1713 c         2117 g         2817 t
ORIGIN      
        1 ggatatccaa actgttttat ctgatctgaa attaaatggt aaagtgattc cagttgttgg
       61 tggcgatgtt aatcagacct atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat
      121 ccatccgaat gtaaaaaaag gattttttga agcagaagta gacggattaa aagaactgtc
      181 agcattcgta cgtgtaccgg atacgtatat gctaggtgaa acaagtgaag gcgcttattt
      241 gttaatggaa tggatcgagc cgggaaaagg ggatcaacga gatcttgctg cagctttagc
      301 taatctccat cagcaaacag cgccacaatt tggttttcgt aaagataact atttgggtac
      361 acttgttcag aaaaattctt ttgaagaaga ttggtggact tttttcttta aagatcggct
      421 tgaatcccag atttctttgg cagaagaaac ggatcgatgg aatgtacagc gtcaagaaaa
      481 gtatctgcga tttaaagaac gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg
      541 cctcttgcac ggagacctgt ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt
      601 ttttgtcgac ccagctgttt cttatgggaa tcgggaacaa gatattgcaa tgagccagtt
      661 attcggtggt ttccgaccgg aatttttaga tgcttatcaa actatatttc cattagaaaa
      721 gggatggaaa gaccgactgc cgatttacca gttatattat cttttggcac atttgaatat
      781 gtttggagaa agttacggtt cccaagtaga tcaattacta gaaaattttt aagaggaggg
      841 cgtgtttttg gcaaaaaaat ataccaaaaa gcatgaagtt tcatattatg agtgtgacat
      901 caaccaaacg atgacatttc cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca
      961 aagcgaccag ttgggaagag gcagtgaatt cgtcaatagc tttggactta cttgggtcat
     1021 cacgaattat tccatggaga ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac
     1081 gactcaagcc atggagtaca ataagttttt ctgttatcgg aacttctgga tccatgatga
     1141 agatgggaaa gaattagtca aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg
     1201 aaaaatgagc agtgttaatg aagagatcat tgctcctttt gagagtgaga agatcaagaa
     1261 aatcaagcgt caggaaaaaa ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt
     1321 tcgtttttat gatatcgata gtaatcagca tgttaataat gcgatgtatt ttaattggat
     1381 cattgacgta ttaggatatg actttttgac tacgcatgtt ccagaaaaag taattattcg
     1441 atttgataaa gaagtagagt acgggaatga gatcgaaagc cattttgaac aaatccctat
     1501 cgaaaatggt gtcaaaacaa aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc
     1561 aaatattata tggaaaaata aataaaaaag aggttgtgac agaagggggc aacttcaaga
     1621 aataagaaga aatttacgaa aattgtttct caaatttttg taaattttcg acttagtttt
     1681 cgaagaagct atttttgtca cagcctctct ttttaagttt agctattaga taatgatgtt
     1741 tagttttgga taaacgaaat caattcttca cccgacaaac catagtttac agcaatcaat
     1801 agttttaaac gtgctttttg actattaata ctgttacaga aaataatccc cattttttct
     1861 agttcttttc ccccgccttc ataatcataa acaggctctg cgattccatt gaaacaacga
     1921 gaaacaagta ctacagggat tttttggaca agaagctttt ccaaagcagc taaggcttgt
     1981 ggagggagat tccccgcacc taaagcttcg attaccaaac cgtctacttt cgtatgggcg
     2041 atggcttcca ataagtcccc ttgcatccca gcatatgctt taacgatcgg gatagtccca
     2101 tctacagcgg aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga
     2161 ttcttagtta ctaagccgat gggacccaat gtaggtgttc ggaaagtagc aacatttgtg
     2221 gtatgggtct ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac caccaaaacc
     2281 cccttatcta gcgcttcttc acaacttgcg acacgaatcg cactttcaaa attataaagg
     2341 ccgtcacttc caagttcatt acttgaacgc attgccccag tcaaaacaat tggtaatttg
     2401 ttgccaatcg ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg
     2461 atcaccactc cggatatacc tgatgcataa gcggacaaga tacgttcttt taataccaac
     2521 atgtgagaag gcatgatgtg tggtgaggga agattgaaga tatcttcaac taataattct
     2581 acatgcttgg ggattgtgat ttctgcatct aataatgggt ttgccacatc aggactaaca
     2641 ccgcccgtga gcatgttttc tttcatggcg atcgttccgc cggtgtgtag tacaagtatt
     2701 ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa tcttattgta
     2761 tactatataa aagagttttg ctatcagtga agaataacga atagcctaac ttatcataaa
     2821 aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac aatggagtat
     2881 ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag gtggccggaa
     2941 atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg taaaaagaat
     3001 aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac tgcctattat
     3061 ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga atcatttcgc
     3121 tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc ttattatcag
     3181 accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt agatgctgga
     3241 aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa agaaaatgtc
     3301 cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat agaattgcgg
     3361 gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat gtctagagcc
     3421 gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat tccttttgat
     3481 ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa tgcgcaagga
     3541 tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta ttcagaaaaa
     3601 gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa agaggtaggt
     3661 gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc actgcaaaaa
     3721 atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt ttaagggctt
     3781 attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga tattgacgga
     3841 acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat tgaatatgct
     3901 cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa gatcaaagaa
     3961 atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact ggtttttact
     4021 gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca tattgttgaa
     4081 tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt agatggatca
     4141 acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt tttaccgcgt
     4201 aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca ccgacaaaag
     4261 gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat attgctcagt
     4321 ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt tcagcgttca
     4381 aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg catccaagct
     4441 ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga tcattacaat
     4501 gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc tcagatcgaa
     4561 gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat atacgaagga
     4621 ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca gtatggaagc
     4681 agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa gaagaccaga
     4741 gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca aaatagaaga
     4801 acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg aagctgttat
     4861 gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa cacagtgggg
     4921 aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact ttacttatgg
     4981 ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc atcaggcaaa
     5041 tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa ataaagtgtt
     5101 gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg cagaaatcca
     5161 acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca tgtgcacaaa
     5221 tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg aaaaacaaaa
     5281 gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag aatagatgtc
     5341 ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc tgttgcataa
     5401 aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca tcggaatcgt
     5461 cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga tgaagaaaaa
     5521 aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg acttgccagt
     5581 gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc aaaatacctt
     5641 tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg aactagaaag
     5701 tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag gaaaaaaagc
     5761 agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg aaatccgtga
     5821 aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc agcaggcact
     5881 tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat acggaccagc
     5941 tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc agtttgtgac
     6001 tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag cagaaaaaca
     6061 tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg agctccatac
     6121 cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga tggaagaaca
     6181 atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc gaagaattga
     6241 aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg aaaaccgtga
     6301 aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg aagctcagag
     6361 atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa caaagaataa
     6421 ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga accacaatga
     6481 aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc aaaatgaaca
     6541 gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca cgttctataa
     6601 aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg acgactcatt
     6661 acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat tcgaattcaa
     6721 attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc cgaatgatta
     6781 cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg tcttgaataa
     6841 aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag aagatctaga
     6901 attattagat aagaagacac atgatttagt agatgcagca gcattaacgg agcaaatgct
     6961 ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa ttgataaaag
     7021 ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa tcggtacagc
     7081 attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt ttaatcatcc
     7141 agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga ttattgtctt
     7201 gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca cctgctcttg
     7261 aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg gtttcttatg
     7321 atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga tgcatatgta
     7381 aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg caatcaagct
     7441 aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt agaaccagaa
     7501 gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa aggaacgatg
     7561 atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca tccggccgtc
     7621 agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc cccagtggat
     7681 aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga gacagtatta
     7741 gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca ggaaatcagt
     7801 gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca ggcgattgga
     7861 aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt ttcagctcat
     7921 aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg tcgtttagca
     7981 ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga aaatgttcca
     8041 ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga acaaaaacct
     8101 gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta tgagaaagtg
     8161 acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc tctaaaagga
     8221 attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt ttctactact
     8281 agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat gcatgtacca
     8341 aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc gcttgcagaa
     8401 gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc aatcaattaa
     8461 aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga actttcgaca
     8521 aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag acaagctttg
     8581 aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct tttattgaat
     8641 ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat ccaaaatttc
     8701 tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt tcaagacatc
     8761 atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag aagcgatcac
     8821 caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt ttttgatatt
     8881 ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt agagattcgg
     8941 cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt gcccgtagga
     9001 acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt agcagggtac
     9061 tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc gccgtataca
     9121 agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc ttatgtggga
     9181 acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa aaagtcagtg
     9241 ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac cgatgaaatt
     9301 cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca agtggcttca
     9361 caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat cattcggcct
     9421 gtagcgacaa tggataaact tgaaatcatt gaagtagcag aacagatcga tacatttaat
     9481 ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca gccaaaaaca
     9541 cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga tgaaatgatc
     9601 caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc taatgaaaaa
     9661 actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg accagagtat
     9721 tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac taatgtcgaa
     9781 gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc ttttttacta
     9841 actgaaaaat aaaattttat ttagagatca agctacagtt gataaatgat ataaaaaaga
     9901 gaatttccca tgaaatagcg aggttgtgac agaagtgggc agcttcaaga aataagaagg
     9961 aatttacgaa aattgctttt caaatttttg tgaaatttcg gcttattttt cgaagaagtt
    10021 acttctggaa caccgtgtat cagaggttgc gaaaaagctg ccctgcatca agtaataaga
    10081 acagccaaac aaaaatagtt cct
//
LOCUS       DADOOS010000100        10091 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.100, whole genome shotgun sequence.
ACCESSION   DADOOS010000100 DADOOS010000000
VERSION     DADOOS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.100"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            274..3501
                     /gene="fss3"
                     /locus_tag="J8C37_002073"
     CDS             274..3501
                     /gene="fss3"
                     /locus_tag="J8C37_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAZ5305499.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3614..3928
                     /locus_tag="J8C37_002074"
     CDS             3614..3928
                     /locus_tag="J8C37_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ5305500.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3941..4315
                     /locus_tag="J8C37_002075"
     CDS             3941..4315
                     /locus_tag="J8C37_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAZ5305501.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4325..4660
                     /locus_tag="J8C37_002076"
     CDS             4325..4660
                     /locus_tag="J8C37_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAZ5305502.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4743..6083
                     /locus_tag="J8C37_002077"
     CDS             4743..6083
                     /locus_tag="J8C37_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAZ5305503.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6161..6835
                     /locus_tag="J8C37_002078"
     CDS             6161..6835
                     /locus_tag="J8C37_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5305504.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            7068..7502
                     /gene="dcm"
                     /locus_tag="J8C37_002079"
     CDS             7068..7502
                     /gene="dcm"
                     /locus_tag="J8C37_002079"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAZ5305505.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7503..8210
                     /locus_tag="J8C37_002080"
     CDS             7503..8210
                     /locus_tag="J8C37_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAZ5305506.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8200..8490
                     /locus_tag="J8C37_002081"
     CDS             8200..8490
                     /locus_tag="J8C37_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305507.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8749..9933
                     /locus_tag="J8C37_002082"
     CDS             8749..9933
                     /locus_tag="J8C37_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAZ5305508.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9948..10067
                     /locus_tag="J8C37_002083"
     CDS             9948..10067
                     /locus_tag="J8C37_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAZ5305509.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3340 a         1690 c         2267 g         2794 t
ORIGIN      
        1 ttcgtatcat tgaataagtt tgaaaaacct tgatttataa aggttttccc gtaaatatat
       61 tttattatat ttgattaaat ttgaaatgtt tgtgttctaa aatgtgttct aatttaaagg
      121 cagtatattt cgtattttag attaaatttt taggctcatt cattctcaaa tccattctat
      181 tcagtcaata aaaattttaa aaagacttta taaacaaaga tgttcaagcg agcatcttat
      241 ttttttgtgc ttaatttgaa aggagaacat ttcatgtata aaaaacaaaa aaagaaatta
      301 tgtgtattaa ctattttgag tttggctgtt caagcaattg ttggagcaat tgctcctacg
      361 attacttttg ctgatgaaat tacccatcca caaacggtaa ccgtagaact agatttagca
      421 catcaatatg ctgttgaagg aacttttagt gatggtcgtc caatgtcaga agtcactgtt
      481 ccgcattatg cagtgtacaa cggctttaaa caagatattt tctgtattga accaggagtc
      541 ccgatttaca atgagttcac tcctggctat gagaaaaacc cgttaccaga tatgtccgat
      601 aaagccaaat tagtttccgt tttatggaaa aaagtaggta cagatgcaga tactcatatt
      661 gtggcacaga agatgatctg gcaagaagtt aatgggtata cccttcactc aataaaacga
      721 tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa aaataaatca agccattgac
      781 gattatcaga agaaaccaac cttccataat agtacggcta aaacagtatt agggcaatcg
      841 actactgtga cggatacgaa taatctgaat ttgtcagagt tcgatgaggt tgtggaaaat
      901 gccgcaaaca ttgattatcg tgtgaatggt aatcagttga taatcacccc aaatgccaat
      961 tcaaaagaaa gtggtgtgct gacgcttaag aaatctgctg gtaccggaac acctgtcgcc
     1021 tataaaaagg ctggccaaca gactttaatg gccggggcga ttgataaacc taatacctac
     1081 acaatcaaaa ttgatgtgga aacggagggt tctttaaaga tcaaaaaagt cgataaagaa
     1141 tcgggtgatg ttgtaccagg aacggttttc catttagact ttggaaaaac tttacctgca
     1201 aaagacgtga ctactgacaa agaaggcatt gctacattgg atgagattcc tcatggtaca
     1261 aaagtaacca ttaccgaaaa atcagtaccg gcgccttata cgattgatac cacccccatg
     1321 actaccacca ttaaagcagg cgaaaccatt tacgtgactt caaaaaacgc acgggaaaaa
     1381 gggcaaatca ttctggataa aagcggagtt gaaacaggga gtgatctttg gaatgacaat
     1441 tattccttag ctggtaatac gtttgccatt cgcaaagaca gtccaaccgg tgaaatcgtt
     1501 caagaaatga ccacggatga aaacggtcat gcggaaacac caaaagagat tgctaatgcg
     1561 ttggaattgg gaacctatta tgtgaccgaa actaaagcta gtcatgggtt cgtgaatacg
     1621 ttcaaacctg taaaagtcga attgaagtat gccaatcaaa ccgtagcccc tgttaccagt
     1681 aacgtaaaag ggcaaaatca agaagtcact ggcgaaacca ctttgacgaa agaagacaaa
     1741 gatactgggg ataaagcaca aggtaaggca gtctttgagg gtgctgaata caccttgttt
     1801 actgctaagg atggaaaagc tgttaaatgg agtgaggctt ttaaaccaga gttagcgaag
     1861 ggaacgaaag gttctgatga aacggtcact ttgactttag acgaaaagaa ccaagttgcc
     1921 gttaaacatt tagctatcaa cgagtattat tggcaagaaa caaaagcgcc cgaaggatat
     1981 agccttgatg aaacaaagta tcctgtatcc atcaaaaagg tggatgatag cgaaaaaaat
     2041 gccgtgatta cccgaaatgt tacggcaaaa gaacaagtga ttcgctttgg ctttgatttc
     2101 tttaaatttg ctggatcagc tgcgggcact accgaaactg gcttcaatga tctgaccttt
     2161 aaagtgtcgc cattggaagg gaccaatgaa atcacaggtg ccgaagatga agcgaccaca
     2221 gcttataacg agcaattagg gtttgatggt tatggcaagt ttgaaaatct tccttatggg
     2281 gattatttac ttgaagaagt agaagcccca gaaggttttc aaaagattac gccattagaa
     2341 atccgttcta cttttaagga aaacaaagaa gactttgtga agagtgagta tgtctttacc
     2401 attaccgaac aagaccaaaa acaaccaatt aaaacggtga ccgttcctta cgagaagctg
     2461 acgaacaaag cattttctgt tagtttgaac cgtttaatgc tctatgattt gcctgaggaa
     2521 gaagatagtt taacttctct tgcgacttgg aaagacggaa acaaagaatt aacttctctg
     2581 gattctaccg agctagttga taaattgagt tacaatctac atgaaatcaa agaagactgg
     2641 tatgtggtag cgcaagccat tgatgtggat gcgacaaaag ctgcccaaga aaaagatgaa
     2701 aaagccaaac cagtggtgat tgcagaaaca agcgcaacgt tggcgaacaa agagaaaaca
     2761 ggaacttgga aaattcagca taaattaacc gccgaacaag ttttaaataa aactattgtc
     2821 ttattcaact atgtgtatga aaataaggaa gcttttgaag caggcgataa gccagtagcg
     2881 aaggatgtta gcttgaataa ccaagcccaa actgtcagtt gtacagtgga acaccatgtt
     2941 tctatccaaa caaaggccca cctagaaaac ggttctcaga cctttactca tggtgatgtg
     3001 gtggatatgt ttgatgatgt gtcgattact catgatgtac tggatggctc aaaagaagct
     3061 ttcgaaacaa ttctgtatgc tttattaccg gatggtacaa acaaagaaat ttggaaatct
     3121 ggcaagattg attatgaagt aaatgataaa gaattcacca aaactgtgct tgcgaaaaaa
     3181 gtagataccg gaaaatatcc agaaggaacc aagtttactt ttgcggaaat caattacgat
     3241 aaagatggga atatcaatgg aaaacacaat gaagatttga aagaaaaatc tcaaacctta
     3301 acaccaaaag aagttccaac catactaagt acgccgaaac aaccggaaac accaactgtt
     3361 ccaagtggct ctcaagaatc tagtcccaca gtgaagacct tccctcaaac cggggagaaa
     3421 aattccaatg ttttactgtt cattggtttt actttgattt ttgcgacggc gggctattat
     3481 ttctggaatc gccggaacta aggtgatgag atgatacaag taaatcctaa gaaccaacgt
     3541 gcgcgaccgc ctcctaaaaa agtagtcgcg ctatttttgt atccaaaact aacaatgaaa
     3601 agaggaaaag aagatggaat tgaaatttgt cgtgccagat atggctgaaa cctttggaaa
     3661 gttaagttat gccggtgaag gagaaattat aacagaagga tatggacgga atactacggt
     3721 gattggtcgc agttatcatt tgtattccag taagcaacgg gccgatgata ttgaggtggt
     3781 agtagctgcg gaagccggtg aaaaggattt tgatcaagac caaccgctaa aagccgtgaa
     3841 tccccatttg gttgccaaag gctatgagat tgagaatcgt gggtttactg attacgtgtt
     3901 gtatgttgat gatttagtga aagcatagga ggaaaagaag atgagattaa cagaaggtat
     3961 tgtcgtagat tcaggtttaa catttgggaa gttgcgtttt tccgcattac gccgagaagt
     4021 acggaagcaa aatgaggatg gaacaattgg taatgaggta aaagaacgaa cctataattt
     4081 aaaatcctct gcacaagggc gaatgattca agttagtatc ccagctaatg tacccttacg
     4141 cgagtttgct tatgatgcag aggtagagtt ggtcaatcca atcgtggata ccgttgccaa
     4201 ttatgttttt cgagaaggaa ctaccgttaa ttggttcatt aaggcggatg atttagtctt
     4261 gaagcggcag ccgaatcaag gaaatcctac gaaccaaaat gaaagaaaga aataggtgaa
     4321 gattatggaa attaatgttg aacatatttt agattgtctc gatcaatatg gaaaaggcga
     4381 gctgactgag gaacaactcg ttaaagcatt aacctctgat gaaaaaatgt ttctgattat
     4441 acatcaaggt ttacttggag aaaatagcga cagtgaagaa gactctgatg tactagactg
     4501 gttaggagaa gaagagtctt tctttatagt tgtcgaagta aatgaaaact tatgtcggca
     4561 ggcagaatcc attttggaag aaattggtgt ggagatgccg gatgctattg aaggcttttt
     4621 aaatcaattg gttgaaacga aacaactccc ctcctcgtag cagtaaatga ctagcataac
     4681 tttttgaaat tgcctaatga aatcctattg ggctttttcg attggaggtg gaaaccatca
     4741 aaatgtacaa aggacatcgc atacgcgcag gagatcagca cttggtttat cactttgttc
     4801 ttggctggct actcgctctg ttcatcggtt ggatgagcgt cttttatttt caagagttta
     4861 gacaatttga tatttctaag ctatcacttt ctactattga aatcgtttgg tctataaaag
     4921 atttagtatg tctgcttgga agtctcgctt tttcaggagc tatgatattg ctttatattc
     4981 acttttttct tgatcattgg cgaagcttgt ggcatcgaca aaagctagct cggatgattc
     5041 tagagaacca ttggtatgaa gtgaaacaaa ctcaaagtga aggctttttc aaagatttga
     5101 atagtagccg aaccagagag actatcagtt acttccccaa aatctgttat cgaatgaaag
     5161 atggattact gtctattcgc gttcaaattt cacttgggaa ataccaagat cagcttttaa
     5221 aattggaaaa gaagttagaa agcggactgt attgtgagtt agtagaaaag gaactcaaag
     5281 actcctatgt ggagtatact ttactgtatg atatgatcgc caatcgaatt gggatagacg
     5341 aagtagtggc agaaaatggg actctgcgat tgatgaaaaa tcaagtatgg gcttatgatt
     5401 ctttacccca tatgttaatc gctggtggca cgggtggagg gaagacctat ttcctcctca
     5461 ccattatcga agcactattg aagtcagatg cagaattgtt tatcctcgat cccaaaaatg
     5521 cagatttagc tgatttaggt acggtgatgc ctcatgttta ttcacaaaag gaagaaattt
     5581 ctgcttgtgt ggaagatttc tatgaacgta tgatggctcg tagcaaggca atgaaagaaa
     5641 tgtctaacta caaaacagga gagaattatg cctatcttgg acttccaccc aactttttaa
     5701 tctttgatga atacgtggct tatatggaga tgttaacgac gaaagaaagt gcggtgattt
     5761 tgaataagct caaacaaatc gtgatgttgg gtcgtcaatc tggtttcttt ctgattttgg
     5821 cttgccaaag gccggatgcg aaatatttag gagacggaat ccgcgatcaa ttcaactttc
     5881 gcgtggcttt gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa
     5941 aaaacttctt tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga
     6001 ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta
     6061 aattagaaaa gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt
     6121 ataatacttt tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta
     6181 tttagagtat gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc
     6241 ccccgaagtt ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga
     6301 ggttgccatt ggattagctg agaattatga taaccatgtg attattgatg cggattactt
     6361 tcgcacccag tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc
     6421 ttggttggta gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga
     6481 tgcaactttt gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt
     6541 tcgagtgacg attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga
     6601 acgtgaaatt gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa
     6661 atcaagagag aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat
     6721 aataaaagat tatacgaata atatttccga agttcattat gctacggata atctagaatt
     6781 atttttacca ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag
     6841 aacaagtaac gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg
     6901 ctactgcaaa agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac
     6961 aacggaaatt agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa
     7021 gagcaaaatg ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag
     7081 atttattcgc cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta
     7141 tcggtttttg tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa
     7201 gtaaggaggt ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg
     7261 gaccagtgga catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc
     7321 ggcaaagatt ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc
     7381 ttctccgacc taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag
     7441 gggctacgtt cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt
     7501 aggtgcttaa ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac
     7561 attctcgaga cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg
     7621 atcaaaacat cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag
     7681 tctatggttc agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac
     7741 tgccagcaag ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta
     7801 atcgagttta tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa
     7861 aaatcatgat tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga
     7921 atctggcttt tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata
     7981 ccttactaac aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa
     8041 cacctagaga gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc
     8101 aagtcaattc agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca
     8161 tttttgatat tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt
     8221 ggagaaattt attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg
     8281 cgacggttaa aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta
     8341 atgaatgagg agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg
     8401 attgcggacc aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag
     8461 ttggaagtaa atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc
     8521 tgaccgcgga ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg
     8581 cgttagccga ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa
     8641 ataagtaagc aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg
     8701 tgttaactca tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg
     8761 aatttagatt atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa
     8821 ttggctacgg cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta
     8881 accgcatcta taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat
     8941 ttgcctttga cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga
     9001 aaaattattc aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc
     9061 ttttactcct atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa
     9121 gaagataaag gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt
     9181 ttactcgctc aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc
     9241 gtgatgaaac gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag
     9301 ctaacgaaaa aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat
     9361 cgttccgggg aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc
     9421 ggcagtctca agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc
     9481 aagttaggga ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa
     9541 aatgaccgtg cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc
     9601 acgttttcca ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga
     9661 accaactggc ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt
     9721 caattaactt ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga
     9781 caagtcgccc ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat
     9841 atccaggata tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa
     9901 caaagtatgg caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa
     9961 aagatatttt tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga
    10021 atgcaagtgc agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc
    10081 ggacaaaatt t
//
LOCUS       DADOOS010000101        10090 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.101, whole genome shotgun sequence.
ACCESSION   DADOOS010000101 DADOOS010000000
VERSION     DADOOS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.101"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            162..392
                     /locus_tag="J8C37_002084"
     CDS             162..392
                     /locus_tag="J8C37_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAZ5305510.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="J8C37_002085"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="J8C37_002085"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAZ5305511.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="J8C37_002086"
     CDS             1803..1979
                     /locus_tag="J8C37_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAZ5305512.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="J8C37_002087"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="J8C37_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAZ5305513.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="J8C37_002088"
     CDS             2474..2941
                     /locus_tag="J8C37_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305514.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="J8C37_002089"
     CDS             3026..3910
                     /locus_tag="J8C37_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5305515.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="J8C37_002090"
     CDS             4027..4260
                     /locus_tag="J8C37_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAZ5305516.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="J8C37_002091"
     tRNA            4348..4428
                     /locus_tag="J8C37_002091"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="J8C37_002092"
     tRNA            4435..4506
                     /locus_tag="J8C37_002092"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="J8C37_002093"
     CDS             5021..5728
                     /locus_tag="J8C37_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305517.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="J8C37_002094"
     CDS             complement(5788..6432)
                     /locus_tag="J8C37_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAZ5305518.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="J8C37_002095"
     CDS             6559..6846
                     /locus_tag="J8C37_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305519.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="J8C37_002096"
     CDS             6954..7274
                     /locus_tag="J8C37_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305520.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="J8C37_002097"
     CDS             7355..8668
                     /locus_tag="J8C37_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAZ5305521.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="J8C37_002098"
     CDS             8665..9384
                     /locus_tag="J8C37_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAZ5305522.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="J8C37_002099"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="J8C37_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAZ5305523.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3363 a         1669 c         2152 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa
//
LOCUS       DADOOS010000102        10086 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.102, whole genome shotgun sequence.
ACCESSION   DADOOS010000102 DADOOS010000000
VERSION     DADOOS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.102"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(247..759)
                     /locus_tag="J8C37_002100"
     CDS             complement(247..759)
                     /locus_tag="J8C37_002100"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAZ5305524.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="J8C37_002101"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="J8C37_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAZ5305525.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="J8C37_002102"
     CDS             complement(3212..3646)
                     /locus_tag="J8C37_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAZ5305526.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="J8C37_002103"
     CDS             complement(3656..4444)
                     /locus_tag="J8C37_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5305527.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="J8C37_002104"
     CDS             complement(4441..5382)
                     /locus_tag="J8C37_002104"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAZ5305528.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="J8C37_002105"
     CDS             complement(5559..6146)
                     /locus_tag="J8C37_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAZ5305529.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="J8C37_002106"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="J8C37_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAZ5305530.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="J8C37_002107"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="J8C37_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAZ5305531.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="J8C37_002108"
     CDS             complement(9063..9998)
                     /locus_tag="J8C37_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5305532.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DADOOS010000103        10017 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.103, whole genome shotgun sequence.
ACCESSION   DADOOS010000103 DADOOS010000000
VERSION     DADOOS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.103"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            165..947
                     /locus_tag="J8C37_002109"
     CDS             165..947
                     /locus_tag="J8C37_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAZ5305533.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1073..1804)
                     /gene="erm(A)"
                     /locus_tag="J8C37_002110"
     CDS             complement(1073..1804)
                     /gene="erm(A)"
                     /locus_tag="J8C37_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAZ5305534.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1862..1949)
                     /locus_tag="J8C37_002111"
                     /pseudo
     CDS             complement(1862..1949)
                     /locus_tag="J8C37_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2313..2975
                     /locus_tag="J8C37_002112"
     CDS             2313..2975
                     /locus_tag="J8C37_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ5305535.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3210..3353
                     /locus_tag="J8C37_002113"
                     /pseudo
     CDS             3210..3353
                     /locus_tag="J8C37_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            3503..3703
                     /locus_tag="J8C37_002114"
     CDS             3503..3703
                     /locus_tag="J8C37_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5305536.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(3957..5636)
                     /locus_tag="J8C37_002115"
     CDS             complement(3957..5636)
                     /locus_tag="J8C37_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAZ5305537.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(5638..5850)
                     /locus_tag="J8C37_002116"
     CDS             complement(5638..5850)
                     /locus_tag="J8C37_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAZ5305538.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            6243..7973
                     /gene="efrA"
                     /locus_tag="J8C37_002117"
     CDS             6243..7973
                     /gene="efrA"
                     /locus_tag="J8C37_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAZ5305539.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            7970..9730
                     /gene="efrB"
                     /locus_tag="J8C37_002118"
     CDS             7970..9730
                     /gene="efrB"
                     /locus_tag="J8C37_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAZ5305540.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            9825..>10017
                     /locus_tag="J8C37_002119"
     CDS             9825..>10017
                     /locus_tag="J8C37_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305541.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDV"
BASE COUNT         3151 a         1662 c         2026 g         3178 t
ORIGIN      
        1 ataaagaata attattaatc tgtagacaaa ttgtgaaagg atgtacttaa acgctaacgg
       61 tcagctttat tgaacagtca tttaggtata tgtccaatct agggtaagta aattgagtat
      121 caatataaac tttatatgaa cataatcagt tacgaggtga aatcatgagc aatttgatta
      181 acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat ttatttggaa
      241 gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta cgcattaaca
      301 gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact cgaaaaaaac
      361 taacagaaag actaatgact atatcaggaa agattggaaa tacggattct gttagaccac
      421 ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct ccaaaaagag
      481 aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt caggaaccaa
      541 gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt atttctctat
      601 ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt cgaagagcaa
      661 ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt aatgtaattt
      721 taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg aaagatgtcg
      781 ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg gatatagcta
      841 gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca aaggtgaaag
      901 cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct aattttattg
      961 caataacagg tgcttacttt taaaactact gatttattga taaatattga acaatttttg
     1021 ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta atttagtgaa
     1081 acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta ttaatattag
     1141 tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga acacgatatt
     1201 cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc tttgaaatca
     1261 atggttgatg tcgttcaaga acaatcaata cagagtctac acttggctta ggatgaaaat
     1321 atagtggtgg tacttttttg agcattttta tatccatctc caccattaat agtaaaccca
     1381 aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata agatagctat
     1441 atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg ttataaggaa
     1501 tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga atatccgttt
     1561 gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga cataagcctc
     1621 catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg gtaaaatgtc
     1681 cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg tgattcaata
     1741 tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg tttttctggt
     1801 tcattataac cttctcctta ctggttataa tgaactgaca ttaagcgttc attataacca
     1861 attaattttg atttggttga taacgaactt tattaataac aaatatagaa aaagtaccca
     1921 tacttttatc tcctttccat ttttttcatt atgaatgaga taaagtaatt tctactactg
     1981 cgatactggt gcacataatt gaaacctcct tatgtaactg aatatgattg tatcatcttt
     2041 tcattttaga ataaaccttt atatgtaatt ttgcacttga cacttcagat ggacgttttc
     2101 ttcaaaataa tatgttttga aacttgataa gagaagccac tgtctttact aaaaagtaaa
     2161 ggctgcgata aaggacagtg actttaataa agttgcactg ttgtattgtc tttggataaa
     2221 attgtctaaa gggaagtttt atccccaacg aaaaaacatc taaagtggta agtaatgaaa
     2281 gaatgtcgat aaagtaggaa tgggggatct ttttgaatac aatagaatca cagtttgata
     2341 aggttgcaga agattacgat ttcgtgaatg agcttttgaa tgattattca ttctttgtgt
     2401 ctaatatgtc tccaaagaaa ggcagagcat tagatatcgg atgtggctcg ggtttgttag
     2461 tggagaaatt agcaagttat tatgatgaag tggtagggat tgatatttct aatcaaatgc
     2521 tcgatcttgc caaatctaaa cgtcaactaa caaatacggt ctatctgaat atgaatgcag
     2581 aacaacttaa ttttaatgag aagtttgatt ttattgtaag ccgaacaacc tttcatcatt
     2641 tggatgatat agccagcgta atacagcaaa tgaaggaact gttgaatgag gaaggaagaa
     2701 tagttattct tgataatgta tctgaagtcg agacaccgcc tacctatgtt tataaattag
     2761 gggcaatcca agagttttta ccgcattgtt tcaaatttgg aataaaaaat gcgattagaa
     2821 tttataatca caacacatct aaatcatggc ttgagcattt agcttcagat aagtacctat
     2881 ctgaacaaaa ttattacgat ttatatgaaa agttgttgcc tggatgccaa ttccataaaa
     2941 tgggttgggc aatgggggtt gtctggacaa aataacttaa agctgtggct ttgaaaagtt
     3001 gaatttgtga ttgttttcag agctatcggg tgctttagta taaataacaa tgaataaaaa
     3061 atcagcctgt atcttatgtt actattaagt aatttaagat acaggtttta ctatttttga
     3121 aataagtacc ttccacttca aatacatgta ttcaaagtga aagtattgat ttaaatgatt
     3181 cttttgtaat ttactcttga taatatgaaa ttccatcgca gcaggatatg aattttacca
     3241 aaaggataca aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg
     3301 gacgcaaacg atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt
     3361 tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag
     3421 ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt
     3481 ttggcacgag gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa
     3541 aggatttgga tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat
     3601 ccaaggtgac ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga
     3661 atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa
     3721 attgatggta gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac
     3781 aagagacaat ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat aaatttaaga
     3841 ggctatgaaa gagccacctt gtatcaagga ataagaacaa acaaaaatgg cttctcatgt
     3901 ttttcatttg ttttctctta tattcaggac aaggtgctga tttcataacc tcttatttat
     3961 tcttcatctg gtgtcattac cattggcaaa atcattggat gtctttctgt acgttcgaat
     4021 aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt tatattctga acagtcaggt
     4081 ccattcaaac attctcttaa agcgtggaat aagattcgct gagcttcatt gatcatttct
     4141 cctgattctc gcatgtagac aaatccacga gagagaatat ctggaccagc caagatttct
     4201 tttttcttga tatccactgt cgcgacagct aatacaagac cttcttctga aagcaggtga
     4261 cggtcacgta aaacgacatt accaatatca ccgatcccgc taccatcgat atacacatct
     4321 ccagccacaa aatgcccagc aggacgcgca gaatcgctag ttaacgctaa aacatcgcca
     4381 ttttccatga tgaagcagtt ttctgcagga acacctacat cttgtgctag agaagcatgt
     4441 actttcaaca tgcggaattc tccgtggaca ggaataaaat attttggctt catcaaacga
     4501 agcattaatt tttgttcttc ttgtccaccg tgaccggaag tatggatgtt attgattttt
     4561 ccgtgaatca cttctgcacc agcttcagac agcaaattga tcaaatgatt gacacttgtt
     4621 gtattaccag gaataggtga acttgaaaag atcactgtat cgtctggctg gatttggatt
     4681 tgacggtgag taccattcgc gatgcggctc aacgcggcca tcgcttctcc ttgagaacct
     4741 gtacatagga tcatcgtttc attggcaggc aagcgattga tttcgtgtgc atctacaaat
     4801 gtgccgtctg gaactttgat atatcctaaa cgttgaccgt tgacgatggc attttccatg
     4861 cttcgtccga aaacagcgat tttacgacca gttgccacag cggcatctgc tgcttgctgc
     4921 aaacggaaga tgttagaagc aaagctggca aaaatgatac gtccttcgat ttttgaaaag
     4981 atcttcaaaa tagaatgccc aatcgttttt tctgatttag taaatgttgg aatctcagca
     5041 tttgtactat cagataaaag gcacaagact ccttcttccc cgatacgagc cattttatgc
     5101 aagtttgctg gttcgccaac aggagtgaaa tcgaatttga agtctccagt cgctacgata
     5161 tttccagaag gtgttttcac cacgatccct aatgcatctg ggatactatg ggtcgttctg
     5221 aaaaagctga cagaagtctt tctaaaacgg atgatcgtat cttcattgat ctcatggagc
     5281 tctgcatctc gaagcaagcc atgctcatct aatttgtttg tgatcaacgc taatgcgaga
     5341 ggaccagcat agataggaat atttgcttgt ttcagcaagt agggtattcc gccgatgtgg
     5401 tcttcgtgcc cgtgagtaat caccaaggct tttactttgt gtaagttttg tatgatgtag
     5461 ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt cttccgggaa tttgatcccg
     5521 gcatcaatca aaatgatttc atcttggaat tgtaccccat acgtattttt cccgatttca
     5581 cctaatccac caatagcaaa gacgcctgtc tcgttatttt tgatggaaag tttcatatta
     5641 aaactccact aatgagaaat cagcattttc tttttcgtac tctagatgac ttccttctag
     5701 aagttgaacg aattcaatat tgtatggggt attttcttcc acacgttggc gagcgtctac
     5761 atcgcttttt gcctcaatgt aaagagattt tgtttcttcc cgtttagggt tgcgtacttt
     5821 tgtttcttga taataaactt tatagatcat tgttttgtct cctttatctg cgtaaagcag
     5881 tgatttagct agttgatttt tttagacaac acaaaacgag gggacaagcc acctggtttt
     5941 tcatatgctt tgactagaat actggattcg agtcaaagag acaagcctcg tttttgctta
     6001 tctgttcgga tattttttca accgtacctt ctgtataaaa aatatacaac aattaattgt
     6061 tattctagca taaaacagca aataagtata gaaatcaact agattgaaag aaaaaatcaa
     6121 tgtttatcat cccgctataa tcataatgac ttttttatga gagaagtgtt ctattaatag
     6181 gctcgttgtg ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga
     6241 aagtgaaact aatgtggcat tataccatgc gttataagaa attcttgttt ttcaatttca
     6301 tatgcgtatt tggttttatt ttgattgaat taggacttcc tacgatttta gcacgtatga
     6361 tcgatgtagg aatccgcaat gatgattttg attatgtgaa gcaacaaggg atatttatga
     6421 tcgttattac tgtaatcggt attattttga atatcttgtt aggttatttt ggttcgagga
     6481 ttacgactaa tatcgtagca gatattcgag atgatttgtt cgaaaagatc caaagttatt
     6541 cgcatcagga atacgaaaat ctaggcgtat cttcattgat cactcgtaca acaaacgatg
     6601 cttatcagat catgttgttc ctgcaaaata ttttacgtat cggttttatg gcaccaatga
     6661 tgttcattgt cagtttgtac atggtcatgc gtacaagtgt gacactcagt ttgtatgtag
     6721 taggtgcatt acctttacta ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt
     6781 tatctaaaaa gcaacaaaaa aacttagata aaatcaacag tatcttgcgg gaaaatcttt
     6841 caggattgcg tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg
     6901 tagtcaatga gaattatgct tctagttcaa aaagcttatt ccgtttgatg gctgttgcac
     6961 aaccgggttt ttatttctta tttaatatcg taatggtatt gatcatctgg agcggggcag
     7021 tgcaaatcga tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc gagtatatat
     7081 tccatgcctt gttctcattc atgttatttg ctagtgtgtt catgatgtat ccaagagcag
     7141 ctgtttcagc tggacgtatc caagaagccc tagatgccaa accttcgatt gtagaagatc
     7201 caaatggtat atctgataca aaaactaaag ggtatatcga atttcaaaat gtaacatttg
     7261 cttatccagg ccattcgcaa gagccagtga tacgaaatgt cagttttaca gcctctccag
     7321 gagagaccgt tgcttttatt ggaagtaccg gtagcggaaa atctacgttg atccaattga
     7381 tccctcgctt ttatgatgtg agtgaaggaa agatcttgat cgatggagta gacgtgagag
     7441 attatcggct aagcaagttg cgagataaga ttgggtttat tccgcaaaaa gcgctgctat
     7501 ttactggaac gattgcagat aatttgcgat acgggaaaga agatgctacg caagaagaaa
     7561 tggaacgtgc agctgagatt gctcaagctt cagaattcat ttctcgaaaa ccagatggat
     7621 ttaatgaaca tttatcagaa ggtggttcca atttttcagg tggacaaaaa caacgattgg
     7681 caatagccag agccatcata cgccgtccgg aaatctatat ttttgatgac agcttttctg
     7741 cattagatta tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat
     7801 ctactgtatt gatcgtagca caacgtgtgg gaacgatcat gcatgcagat aagatcatcg
     7861 tattaaatga aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc
     7921 cgatttatta tgatattgcc gcttctcaat tatcaaagga ggaattagca tgaaaaaaac
     7981 cttttcttcc ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga cttttctttt
     8041 agtgatccta tttacagttc tgacagtggc tttcaacgtg gctttgccat atgttacagg
     8101 acttcctact acagaaatca gccgtaatat tgccaatggc gaatcaatca atttttctta
     8161 tatattcaaa tgtctcattt ggatcacagt tgttggaata gggtattgta tttcgcaatt
     8221 actttctggt gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga
     8281 catagaagaa aaaatcaatc gtcttccggt ttcatatttc gataaaaacc agcaaggaaa
     8341 tattttgtct cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac aacaagcttt
     8401 tattggtatc gtaaatgcga tattgggaat tacgttggct gcagcaatga tgttttatat
     8461 ccagccgatg atggcgctga tctctatgat catgattcca acatcgatct ggatctcaaa
     8521 acgagtgatc aatgcttcgc agaaatattt tcaaagcatg cagaattctc ttggtgagct
     8581 aaacggctac gtccaagaaa atatgacagg ttttagtgta ttgaaagtat atggacgcga
     8641 aaaagaaaca ttggaaggat tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg
     8701 tgcagcattc atttctggct tgatgatgcc tctagttcaa ttaactgcct atgctactta
     8761 tattggtatg gctgttctag gaagctttta tgttattact ggggcgatcg ttgtcggtca
     8821 actacaagca ttcatccaat atatttggca aatcagtcag ccaatgggga acgtgacaca
     8881 attgtcttca gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga
     8941 tgagccagaa gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga ttttaggaga
     9001 tgtgacattc gatcatgtcg attttgcata tgatccgaag aaaccattaa tccaagattt
     9061 gaattttgaa gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa
     9121 gacgacattg atcaatctat tgatgcgttt ttatgacgta gacaaaggag caataaggat
     9181 tgatggaatc gacaccaaat caatgagtcg cagtgatgtg cgttcgctat ttggtatggt
     9241 cttgcaagat gcatggcttt atcaaggaac gattgcagat aatatccgct tcggaaagct
     9301 tgatgcgaca gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag atcattttat
     9361 ccgaacgatg ccagatggat atgatatggc catcaattct gagggagaca atgtgtcttt
     9421 aggacaaaaa cagttgctga cgattgcccg agcagtggtc tccgatccta aaatcttgat
     9481 tttagatgaa gcaacaagtt cagtagacac ccgtttagaa gcgttgattc aaaaagcaat
     9541 ggatcgcgtg atggagggac ggacaagttt cgtcatcgct caccgtttat cgacaatccg
     9601 tgatgcagat ctgattctag tgatggatca aggtcagatc attgaaaaag gaacgcatga
     9661 gagtttattg gcacaaggtg gattttatga aaaactgtat aacagccaat ttgctgagga
     9721 agcagaataa gtcatataag aataaaacag tttaaaactt attttttgtt ctctattctt
     9781 gataatggta aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa cagtttttaa
     9841 atgaaaaaat tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga
     9901 agccttttga ttcagctgat ttttttgaaa atgagtttca aatcgaacgc ttggcagcac
     9961 aaatagaagt gtacgaatta attttaaaaa aagtaataga agaaaatata gatgtac
//
LOCUS       DADOOS010000104         9872 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.104, whole genome shotgun sequence.
ACCESSION   DADOOS010000104 DADOOS010000000
VERSION     DADOOS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.104"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            178..2505
                     /locus_tag="J8C37_002120"
     CDS             178..2505
                     /locus_tag="J8C37_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ5305542.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2631..2960
                     /locus_tag="J8C37_002121"
     CDS             2631..2960
                     /locus_tag="J8C37_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAZ5305543.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            3000..4004
                     /locus_tag="J8C37_002122"
     CDS             3000..4004
                     /locus_tag="J8C37_002122"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAZ5305544.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            4001..4447
                     /locus_tag="J8C37_002123"
     CDS             4001..4447
                     /locus_tag="J8C37_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAZ5305545.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4597..5412
                     /locus_tag="J8C37_002124"
     CDS             4597..5412
                     /locus_tag="J8C37_002124"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAZ5305546.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5618..6790
                     /locus_tag="J8C37_002125"
     CDS             5618..6790
                     /locus_tag="J8C37_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAZ5305547.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6952..7446)
                     /locus_tag="J8C37_002126"
     CDS             complement(6952..7446)
                     /locus_tag="J8C37_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305548.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            8029..8451
                     /locus_tag="J8C37_002127"
     CDS             8029..8451
                     /locus_tag="J8C37_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ5305549.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8617..9810)
                     /locus_tag="J8C37_002128"
     CDS             complement(8617..9810)
                     /locus_tag="J8C37_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5305550.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT         3402 a         1879 c         1884 g         2707 t
ORIGIN      
        1 aaaaagagta ttttttattt aaaaataaaa tctttgtatt aaaggacaag actaaaataa
       61 ccttagaata aaaagagaaa aaaagcccca aactttctat acatcaataa aataataaaa
      121 attaaattta gaataacgtg taaaggatta tttgatgatt ttttattgag aggagggatg
      181 aaaatgatta agaaaactca aaataaaatc caacgtatat gtcaaaaaca ttttgtttct
      241 agactcaaag ctgtatctaa aagaaaatac ttacatccca ttatgatttc gatcatcttg
      301 atttttactt tcttagggag cttatcaagt atttatgtaa acgtttatac agaaacgtca
      361 aattttgcta accaaacgac agaggagaca aatagtccac cctattctga aatacagtcc
      421 atagaaagca ccaatcagtc tagcaacaac gagacaacct caaccacatc ctcgactcaa
      481 ctgattgatc cgacagaaaa agaaatcaca gcagcaaaag cagcagggga gaaagcttat
      541 tcagaaacag gtagaagcca gacatttagt actgttgcgg ccactgcgaa tttcaacgga
      601 cataccatac cagatggata tacagtgata tatgccaaag atggagtcat tgttgcggtt
      661 aacggatcga tgagtgcgga taaaactgcg ttacaaggcg taaccaaagc aatcgctttt
      721 attgataacc gtccagatat ccataatctg tttattggct ccacagtaaa tggtactact
      781 atcacattcc caaaaagtat ctatatgggg gtggctaccg ctggaaatac gatcgattca
      841 gttacaccca atgcggataa aagttattct tttacaccct caacatgggg agggcttcct
      901 gccggaggag gtatgagtac tttttcaatc ggtatacagt tagcaaatga tattccattc
      961 acggggtact cgatcaaaaa tgggcagatc gttgatggta agggaaatgt ttcaaatttt
     1021 ccaacctatc aagcagcaac ggttcaggtc aatgcgaatc ggacaggaac tttttcttat
     1081 acagtcaatg gtggagcagc aaaaatcgga acgtacggga ctacattatc tggaatccat
     1141 gtgggtgaca aagtggttat cactccaaca gcagtaacgg gctattcttt tgcacaaaca
     1201 aacggttctt tcacagccct agaaagtaac gtcgatacgg tgacctatac aggcaaacaa
     1261 acaccaatca ctattaagta tcaagactat tttggacaga tgattgctcc ttctaaaata
     1321 atgaatctta cttacggaag tgctgcacaa gacctgacga ctaatacgcc tactatcaac
     1381 ggatatacct ttacagctgt ttcagcgata gaaacaaaga accaatcagc aatgagtgtt
     1441 ttagctagtt tagatacaaa tggaaatgta atcgtaaagg acgcaaatgg aaaacagatt
     1501 tctgaagtca tcctatatta taagacaaat gcaacagtat caatcaatgc aaatggcagt
     1561 aagtactatg atggtctttc tgttgtaccg atcgttaaat atgcctttaa tgataaaaca
     1621 gaatcaacat cccttttaaa caatgatctc agtattgatc aaatcacatg gaatccaact
     1681 gatttcaaag cgatgaatga aaagggagtt gaagtaactg cgcctactga aattggaacc
     1741 tatacgtctt ggcaattgac aaaaagcgga ctagctaaac tagctgccag aacaaactat
     1801 ttatttacta tcgtgcagac atctgatgtc tatacgatta agcaaatcag cggtgcggta
     1861 gagctcagtg gcagtaagac atatgatgga aaagcaggtg tcccgtcaat ccatgtcaag
     1921 ctagcagagg gtgtcactag ttcgcttact ccagtacaag tggcacttag cagtatggat
     1981 tacacagcag atgcacaatc agcaaaaaat atggttaatg ctggcagcta tacaattaaa
     2041 ctaacgaata gcggaataga caaagtcaaa gcagcaaatc ccaagttgtc atttactgat
     2101 atagccaaca catcaggaac ttataccatc tataaagcta acgcagtgat tactgtcgat
     2161 gacgctgcct ttaattatga tgcccaaagt cactcgattt cggttggaaa tctccacgtt
     2221 accggtgttg tcttaggcga atcattggat tataccctaa cagttaacag ccggacagat
     2281 attggatcac aaatagtaac gattagttta ggcgaaggaa gtgtaaacaa taattatacg
     2341 attatcgcaa cagataccgc taagttgacg atcaatccga ctctcttgct tccttcgaca
     2401 ggtgggatag gtctactgcc ctttgtcttt ttaggactga tctttatttt ttctggcttc
     2461 ttttatttta tccatcgtaa gaaaaaagca ggtgaacagc gatgaaaaaa acaaaaaatg
     2521 tacgtgtgga tatatttgct gttctctttt ttatcctact ggtctttttt ataggttctc
     2581 cgactgtgtc agctagagaa tcacctatta catttacttt gcataatcga atgttcgaaa
     2641 gcgataagat gccaagtatc agtcaaaacg acggaataaa tatcccggat aactttcaag
     2701 gagtaaaagg gttgaacggc gttactttcg ctatatatga tgtctcggat gaattttaca
     2761 agctgcggtc agaaggaagt agtgtggaag acgcacaaag aaaactagca caaaaaagtg
     2821 atagtgaaaa aatactggca gaaaatgtga caaaaacagt agacgaggaa gagggcattg
     2881 cttcttttag tgcatcggac aaagatgagc aaggtcgtga cgctgtttac cgatttacgg
     2941 aaataaagac ttcagattaa gtaaaagaaa aatcagcacc atttgtcgtt gtgcttcctg
     3001 tgacagattc atctaatcat aaattgacga ccatccactt gtatccaaag agcgaacaaa
     3061 aaatacctgc tgcactcaca ttaacaaaaa cagtagagaa taagcagaca gattttgcag
     3121 atggggataa agtcccttat ctcatcaccg taacaattcc tgagaacatc aacgagatag
     3181 gaacctatac gatcaaagat acagctgatc ctcagttatg gctagaacca gaaacgatcg
     3241 attttttgat tggtggagac aagattcata catttcacac acaaaaaata aaacatggtt
     3301 ttgtcttatc agattctgga aaagatctta gttcgtttgc tggaaaaaaa ttaacgatcc
     3361 gttaccaaat gacattgaaa gaaaacagtg aaaaaacgac atttgataac gatgtacgtt
     3421 tgacgactga caatcaaaca gtagaaacac atcttgccat ccaaacagga ggcaaacaat
     3481 ttgtcaaagt ggatcggcaa acgaaacaaa aactagcaga tgctcaattt ctagtcaaaa
     3541 aaaatgacca atacttagcg caagaaaacg ggataaatca ttgggtagcc aaagatgatt
     3601 caagagcaac gatttttact tccagtaaag atggaacatt ttctgtccat ggattgtctt
     3661 ttggaagtta cgaactagtg gagattaaac cgcctaaagg atatatattg agtcaatcaa
     3721 ccattctctt tgaagttgaa aaaggcagtt atgatccgct tcatcctata ccattggaaa
     3781 tcatcaacga accaaaagca aatccgacga ataatggaaa aacgccagtt cagatgcaga
     3841 caaatggtcc atcaaatgaa aaaaatggag gaagctttcc aaaaacaagc gaagagatgc
     3901 ttggaggttt ctcaatttta gggttattgc tagtcctttc aacagggaca gcttggtttt
     3961 ataaaaaaca acaaaaagga aacaatagga gggaaataaa atgaaaaaca tcaaaacaaa
     4021 aatatggacg tttttaggaa ctgctatcat gctgcttcct ttcgttctcg gcctaggaac
     4081 tgcagaggtt tctgcagccg tatcacctac accagaaaat gttacggtta atttgcataa
     4141 attgaaattc acttcagcac cagaaaatca aataaacaac ggaacggaat tgacatttcc
     4201 gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat gacatcactg ctacgtacta
     4261 tccaagtaaa gacacagcag ttccagcaga cgctacaccc ttcgcaagtg taacgacttc
     4321 tggtgaagga ttagctaacc tgactcttcc aggtaaatct gatgggaaag atgctgtata
     4381 cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg ccaaatatcg tgttaagctt
     4441 accctagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4501 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4561 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4621 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4681 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4741 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4801 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4861 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4921 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4981 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     5041 aaccttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5101 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5161 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5221 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5281 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5341 ttggtaggta ctgctttagt tggaggagca gtgatctatt tcaaaaaacg ccatcaagaa
     5401 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5461 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5521 taggaggaac tattttgccg tttgcttcgc taatactcag gtcaaggcgc tgatttcaca
     5581 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5641 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5701 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5761 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5821 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5881 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5941 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     6001 tttgcaaaaa ggaacgtcat tattggaagg aagttcttat ccgactggcg ggaaaaacac
     6061 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6121 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6181 agtagaaaaa atccaagtgg tcttacctga cgaagtagat tctttgggta tccaaaaagg
     6241 cagagatctc gtgactttgc ttacctgcac tccttatatg gtcaatacac atcgactgct
     6301 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6361 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6421 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6481 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6541 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6601 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6661 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6721 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6781 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6841 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6901 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6961 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     7021 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7081 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagaag cctgttcagc
     7141 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gcctcgcctg
     7201 ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt tctgcttgtt gtcgtgcaat
     7261 ttcttcttgc tttttccgtt cttcttctgc ttcttttgct ttgattgtgg aatcaattgc
     7321 agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc gcttggatag ctgctgaagc
     7381 agttgcatag ttttcttttg taggatttgc ttcagcttgt gacaaagcag attccgcttc
     7441 tgtcactttt ttttctagtg cagctttttc cgctttttgt ttctcttctt cttttttctt
     7501 ttcaatttgt tctttcgatg aatctgccgt actttcggag atcgtggtac tgctgctgga
     7561 agcctgagct gaacttgtgc tttgagtagt agacgtagta gcaccacacc cactcaacaa
     7621 acttcctgcg accaaaaaca acgcaactaa ttttttcact aaatttcctc cttaagtaaa
     7681 aaacaaccgt tttcaataaa tgtccttttc aattgtttac ttacttgtta taataattgt
     7741 gttatgaata gtgattgagg aaacttaatc taatcccgtg ttgatacacg ggattttttg
     7801 ctatagttct gttattaagt atgcaataat aaaatatgta cgttatttta ttattagatt
     7861 attgacattt atataataaa cgattaaatg attcatatca atagaatact taggaaaaaa
     7921 agagaaataa taacaggttt atgcactatt gacggtgtga caatatgata tttgtatccg
     7981 tttttttctt gtatgtagta tagtaaagta gatacaggag gagaaaatat gcagactttt
     8041 ttaacaaaag tttctcaaaa gaaaattaaa atgattcatt tactgcttga aacagaacgt
     8101 tggtgttcaa tagaagaact acaaaacgaa ttgaaggtat caagtaagtc tatcttgaat
     8161 tatctaacag atttagaaga attgttccaa caatatcctg ataaggtcat tttgaagaat
     8221 gagaataatc ggcgcttttc tatggaaaag caggagaact tcccaatcta cattatttat
     8281 cttcatttct acagaaaatc atataattat catttgattg aattcatgta tcaacatcct
     8341 gagaaaaatt tggaagatta tgcagacgct caatatacga gtgtatccac agtttttcgt
     8401 tatgctaagt tattagtaaa gtactttgaa atgacggacg tcaaagaata gtcgccgcca
     8461 gatagaagga aattagcagt tgaaaaagct gccctgcatc aagtaatacg accagccaaa
     8521 cggaaacagt gtctcatgtt tttgatttgg ctggtcttat taccgcagat gcaagctttt
     8581 tccacacctc tttcttgtca tttacagact caactatcaa tgctgcaagc tttcccttaa
     8641 tgctgtaaca ccagtaagag ttgtcaagac cagcagactg actaggtaaa taccgctcaa
     8701 aaacaagatt acggcagtta aactataatg atccatcaag aaaccgatga cgatagaaga
     8761 aaaaccacca acagctcgtc caaggttcat aacgaaatta ttcgccgtcg cgcggatttc
     8821 tgttggatac aaactactaa tcactgcgcc atatccacca tacatgccat tgataaagta
     8881 gccgactatc acagcggctg ctaccaaact ccactggcta tgggctaaca caagtagata
     8941 gacagataag gatgaacaaa ttaaaaacaa agaaaaggct agtttaggcc ccaatttatc
     9001 catgattatg ccaaaagtca tcatccccaa agacatgcct aaaatagtac tgaccatcca
     9061 caatgaagac ccagaaaccg aaagccctaa tcgattttgc ataattgaag gtagccaatt
     9121 cattaaccca aaatagccgg caatttggac agttaccatg atactaaggc cacatgtttg
     9181 ccaagcaaga tgttttgatt caaacagtct gctaagtttg gttttctttt cggcagattg
     9241 tacagatttg aattcctttg gttcagtcaa gccgcgacga atgaacaaga ccatcaacac
     9301 cggcagtaca cctacgacat acaacatctt ccaaccaaac gttggaatga tcacagcagc
     9361 taaaatcgca gccaaagccg caccgacctg cgcaccaata ccagcaaccg aagaagctcg
     9421 tccaatttgt ttttttgaaa aactttcgga aatcaaggac atacacgccc catattctcc
     9481 tcccgctcct acacctgcga taaagcgaaa gagataaacc aagtagatgt ttgaagcaaa
     9541 atacatcaat aaagaagcaa tcgaaaacag aaggaccgtt tgagaaaata ctttcacacg
     9601 tccatattta tcagctatga tgccaaaaaa aatacctcca atgagcatcc ccaaattagt
     9661 gatcgttgaa atgaaacccg cttgcgtacc actaatatga aaagaagtaa tcattgaaga
     9721 taatgaaaaa gctaaaaaca taatatccat attctctaaa gcaattcccg ccgatgtcga
     9781 tactaatacc tttcgctgat acgttgtcat ttttataccc cttttcctaa aatttcccta
     9841 aattgttaaa agtatatcca aaaaagaata aa
//
LOCUS       DADOOS010000105         9574 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.105, whole genome shotgun sequence.
ACCESSION   DADOOS010000105 DADOOS010000000
VERSION     DADOOS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.105"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            100..294
                     /locus_tag="J8C37_002129"
     CDS             100..294
                     /locus_tag="J8C37_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305551.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            563..862
                     /locus_tag="J8C37_002130"
     CDS             563..862
                     /locus_tag="J8C37_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305552.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(959..1393)
                     /locus_tag="J8C37_002131"
     CDS             complement(959..1393)
                     /locus_tag="J8C37_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305553.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1625..2335)
                     /locus_tag="J8C37_002132"
     CDS             complement(1625..2335)
                     /locus_tag="J8C37_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAZ5305554.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2325..3185)
                     /locus_tag="J8C37_002133"
     CDS             complement(2325..3185)
                     /locus_tag="J8C37_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAZ5305555.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3190..3828)
                     /locus_tag="J8C37_002134"
     CDS             complement(3190..3828)
                     /locus_tag="J8C37_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAZ5305556.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3843..4142)
                     /locus_tag="J8C37_002135"
     CDS             complement(3843..4142)
                     /locus_tag="J8C37_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305557.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4185..5669)
                     /locus_tag="J8C37_002136"
     CDS             complement(4185..5669)
                     /locus_tag="J8C37_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAZ5305558.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5689..6150)
                     /locus_tag="J8C37_002137"
     CDS             complement(5689..6150)
                     /locus_tag="J8C37_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5305559.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="J8C37_002138"
     CDS             complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="J8C37_002138"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAZ5305560.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7453..8223
                     /locus_tag="J8C37_002139"
     CDS             7453..8223
                     /locus_tag="J8C37_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ5305561.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8496..9323)
                     /locus_tag="J8C37_002140"
     CDS             complement(8496..9323)
                     /locus_tag="J8C37_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5305562.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2984 a         1951 c         1498 g         3141 t
ORIGIN      
        1 ttgattcttt ttatttatca taaagatata gatacataat cattttcact tacatatatt
       61 tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt agaagtaaaa
      121 acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata tcctttactt
      181 ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat aaagaacgag
      241 ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa gtaaatccaa
      301 tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga ttatagaatt
      361 agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat accatatgat
      421 tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga tgaatcaaat
      481 atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt tataatgtat
      541 cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg aactacttaa
      601 gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca aagtgacaga
      661 gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg caaaaggaat
      721 atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg ctttaaaaaa
      781 aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat ttgataaaga
      841 ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat actaagtcta
      901 tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt attatatgtt
      961 atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa gaaataccac
     1021 ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt ttctcagtat
     1081 catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc aaagcttcat
     1141 tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg atatcttgaa
     1201 taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc ttcccttccc
     1261 cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt gcagtgtctt
     1321 ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg atcataaggg
     1381 aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata atacagttct
     1441 ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat tccttaaaat
     1501 tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga taaattttct
     1561 tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc ttaccctagc
     1621 atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt ttatctagta
     1681 aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg gccatcagac
     1741 aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc ccccatgtga
     1801 acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat tttctttcct
     1861 taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt acttcttctt
     1921 tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga gtcgtgccat
     1981 aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa tgagtatgag
     2041 taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg agatctgaag
     2101 atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc accatttgat
     2161 ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact cctagctcac
     2221 ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc ggcaatgcca
     2281 gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa gacatcatag
     2341 ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc tcttcgaaag
     2401 tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc tagacgtttc
     2461 aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac atcacgaaac
     2521 tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc agtgatataa
     2581 tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga caaattgcct
     2641 aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc taaaaatttt
     2701 gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat gccatattta
     2761 gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga attcattgat
     2821 ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct tagattatgc
     2881 gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc tggatcagca
     2941 gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg tacaccggag
     3001 ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa tcgtgctaac
     3061 cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc ttttacaagg
     3121 gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat cccaatttga
     3181 gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg cggccttcat
     3241 cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat cgatcccttc
     3301 aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa agcctagttc
     3361 aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag ataacagcgc
     3421 atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat ccatggcttg
     3481 ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt tttgtgctgc
     3541 ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc ctgcttttgc
     3601 aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga ctagtttttt
     3661 atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta agcaaagaat
     3721 tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc ctaatgcatg
     3781 ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct tagttccttc
     3841 ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat ttctttgact
     3901 gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg agtaaacata
     3961 ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact ttttccctct
     4021 gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat tgctttttcc
     4081 acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac taaaattctc
     4141 atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac tctctgcttt
     4201 tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt tttccgatat
     4261 tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc ataacctagg
     4321 actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat cccgatataa
     4381 ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc tacttgtatg
     4441 attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt attggcataa
     4501 actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac gatcgttggt
     4561 gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc aaacaagaac
     4621 ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc aattcctgga
     4681 acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag ttcatcgacg
     4741 aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa agaagtcgtc
     4801 ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat caaataatct
     4861 ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga agtagcaacc
     4921 atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat ttttttgtct
     4981 tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc taattttgca
     5041 aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc ttctgtcaat
     5101 tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc taaaatcagt
     5161 cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag taaaatccaa
     5221 aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga acgcagtttc
     5281 gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag taataaaatc
     5341 atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac agcattttgt
     5401 ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag tccaaccaag
     5461 attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa gtaaccgacc
     5521 gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa aatatagcct
     5581 aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt tgcaaaatac
     5641 gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt aattatattc
     5701 tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct caaccaatcc
     5761 atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt ctggatcttt
     5821 tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt cactttcaaa
     5881 acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac catcactttt
     5941 ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac cgtatttttc
     6001 tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat cgatcaatac
     6061 aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg ggtaatgaat
     6121 caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta taaaaacgac
     6181 ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata aaaattaaac
     6241 tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca gatataaggt
     6301 ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat ctcttttgga
     6361 tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt tgcattcaat
     6421 gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc tcgtgggttt
     6481 tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt ggcaaataaa
     6541 ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc gaataaatag
     6601 tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc ttctggatct
     6661 tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt gatgtctttg
     6721 attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat tccccatttt
     6781 tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca attttgatga
     6841 actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac gattaaagcg
     6901 tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa attaggctgc
     6961 atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat gccattccca
     7021 tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc ttcatgactt
     7081 ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg atccactact
     7141 tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc tactaaccac
     7201 ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc atatcgttgt
     7261 tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt ttatatgacc
     7321 gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat aataaagaca
     7381 gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga tgtccctcag
     7441 gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa agaaattttg
     7501 gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc ccttgatgta
     7561 tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga gatcatacgc
     7621 aattacggtt attgttctta taattacaaa aaccagactg attttgatca atctggtcct
     7681 gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga ttatgatacg
     7741 ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca atctaaccat
     7801 ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga taattgtagt
     7861 tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg cgacatcgca
     7921 ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag cggagtcgat
     7981 gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga aatgatggtc
     8041 aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg actgacatca
     8101 aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa attttgttct
     8161 gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt agattcattt
     8221 taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa caaatttatc
     8281 gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg tatccatcca
     8341 atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc taaataggaa
     8401 tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt tcgaataaac
     8461 ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt ttcaatcacc
     8521 tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat atatctttct
     8581 aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag caagtattcc
     8641 aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca taattggatt
     8701 gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata tacaatgcag
     8761 accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc cacataaact
     8821 ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc agctgcttgt
     8881 aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata aaataatgct
     8941 gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa aatttgattt
     9001 aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt ataagattct
     9061 tggtattttg atacagaatt caatgattga atctcattta tttttttcct gattttgtct
     9121 tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca aagcgcactt
     9181 aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt tgcttgttca
     9241 caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta ttcttttagt
     9301 aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta tttctccttt
     9361 atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga ataaaaattt
     9421 taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga tttctttttg
     9481 tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt catggcgaga
     9541 aagtctaaga atatttttaa acataactat ttct
//
LOCUS       DADOOS010000106         9402 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.106, whole genome shotgun sequence.
ACCESSION   DADOOS010000106 DADOOS010000000
VERSION     DADOOS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.106"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(16..438)
                     /locus_tag="J8C37_002141"
     CDS             complement(16..438)
                     /locus_tag="J8C37_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ5305563.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(428..2602)
                     /locus_tag="J8C37_002142"
     CDS             complement(428..2602)
                     /locus_tag="J8C37_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAZ5305564.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2618..4960)
                     /locus_tag="J8C37_002143"
     CDS             complement(2618..4960)
                     /locus_tag="J8C37_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305565.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4982..7525)
                     /locus_tag="J8C37_002144"
     CDS             complement(4982..7525)
                     /locus_tag="J8C37_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAZ5305566.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7535..7933)
                     /locus_tag="J8C37_002145"
     CDS             complement(7535..7933)
                     /locus_tag="J8C37_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305567.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8014..8298)
                     /locus_tag="J8C37_002146"
     CDS             complement(8014..8298)
                     /locus_tag="J8C37_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305568.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8322..9320)
                     /locus_tag="J8C37_002147"
     CDS             complement(8322..9320)
                     /locus_tag="J8C37_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305569.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2678 a         2010 c         1468 g         3246 t
ORIGIN      
        1 ctctttcaat tttcactaaa aattaggaag aaattgattc aatgtctttt caaaaaagaa
       61 tgtttttctc tcaatttttt tgacgtttct atcatgaatc acgagaaaac ttgggacact
      121 tttaacatgg taacgttgta aaaaagtttg aagttcttct tgtcttggtt cttttcttgt
      181 ttcaattcga tatacattcc tagagaattt tttgtcgtat ctttccaaga aagattcaac
      241 ttcttggcat tttttacaat catttcttgc tagatacaca aaaaatgatt gattcgtttt
      301 ttgaagttct agtacttctt ctaaattact attgattagt tttccatttg attcttcgtt
      361 agtttttatc agtttaaaac aaccaaataa gaggaaacta acaataagta tcctaacaat
      421 aactttctta tttcgcatat tgtttctcca aatttggttt ccgaatatag acgactcgcg
      481 gtggttcctg gacgtcttca gctgttcgat acaccacaaa ttcgcctgtt ctcaatgctc
      541 gaatgcgatt cggattgaat ttaaagcggt ctacgtctcg tttcgtgcct ctttctcctg
      601 tccaatcaac ctttcccata tcagaataac ctgcttcttg tgtcgtcatt tccgttaatt
      661 caatgtcttc ttctgtcccc attaaatctg cccaataatc aatttctgtc gggtcatttg
      721 tcttcccaat aaagaacgta ttcacattcc caatcaactg ttttcgaagg acaggatcaa
      781 ttctatcaat gtccgcaagc gtttgagggg catacgccgc atgcacccca gcgcctcgtc
      841 ccttattgac taagtctata aggttatcat ttagataaga agctggttcg tcaaatatta
      901 ctaggacttt cgagcgattg gctgtttctc ctgcttgttt atacacttct gacgcaaaga
      961 aattaatatc tcccacaagc atttgagcca atgtccgaat aaactcacta taaatcaatc
     1021 cgtttaacga gatataaaca atctttcctt ctttaacaac ttcttgaata tctaattctc
     1081 tttttcctcc agaagtatca aagagttcac caagctcact atccaataac atattgatat
     1141 tggtctgtaa attctcagaa gtcgcataca aataaaacgg gttcttctta tgttcgtaac
     1201 gaacaaacaa ccgtttaaac aacgtttgct cgatttctgt catgagagaa atgttttctt
     1261 ttaataatag ataatacgtt tctaagtcaa cggttttacg atcatgttca acgattcgct
     1321 cttcacttaa ctgttccaca tcggcaaaat cacgttctgt tttgtccatc gcttcattct
     1381 ttttcgtttt tttcttcgtg gttttcttct tttctacgac cactttttct ttaaataaat
     1441 ctggattttt caactgaata cgatccgcaa acaaatgtaa gacgtttcgt ggcaataaat
     1501 aatgttgtaa ccaaactaga tttctttcaa ttttatggct cttttggaac tcgtctaaaa
     1561 gctgaatcgt tacctgtaat aacgctttgg ctgctgtcga ataatacacg ctttctgttt
     1621 ctgctagatt ggttaattta tctcgaatgg caatatcatt gccatttttt acaggattat
     1681 aacgcaaatc gccaccgtcc acaaagactt caatcgggcg attgtatgcc tctgccactt
     1741 cttttaacgc ttgtaaagtc tctcctgctc ctttcccatc gactaagata acggggatac
     1801 gaaacttaca cgcatgttct gctagtaatt ggatcaccgt cgtttttcct gaacctgtag
     1861 tcgctggaat aatcacatgt tggttcaatt cactaaagtt catcacataa gggagcccat
     1921 actcatctgt tcctaataga atcactttgg ctaatgaagc cagttgttta ttccgcaaag
     1981 caacgtcttt tatgacaatt gctacctggt acgcttgctg atactctgct gtatattttt
     2041 gggactcctg aaatttattc ttccgacgtt ccttgtattc tttactattt agcaaagctg
     2101 tttgccgttc tttctttgaa acaacttttc ttttttcaat aaaagacgca tacgcaatca
     2161 aacaacttcc tgcacctgtc ccacctaaaa ggagggcaaa aatcgtttgt ccattaaact
     2221 gcaacgcatg agaaaacaag tttaatggaa ataataagta acacgacaaa taccctctag
     2281 gaagaacaaa aataaaacaa aatagattta atagcgtaaa acctaacgca aaaataaaaa
     2341 aaagagagct tcttcgtttt aagaagctct catactcaaa gtcataaata tgtgcacgtg
     2401 tctctttttt atctatcata aagacaatcg ctaaaggaat actgcctaca ataaaaaatg
     2461 gatatagaca aacacctaat aaagtgacga taaaataaag ttgttgaaac cgactttttg
     2521 gcgtttcttt ttcttgtttt cttttgcgat cgacagaaaa catttgctca aaatgtgtcg
     2581 gtcgttttgg tgcattcatc atactatcga tttcctccta tcgttttcgt tgtttcattt
     2641 ctcttcgata aatcggttgt ttttctcgtt ttcgtttcat ttttgcttga gttgctcgaa
     2701 actcactccg tgtcttcggt gcttgttttg gtgattttgt ttgtttcatg gttgagtatg
     2761 ctttagatac ggttgaaggc gctttggttt gtgctgttac tcgattgact gctgacatat
     2821 gttcccgaac cccctctgga cgtcgaacag atttcggtgt ggtagaacct gttgctcgtg
     2881 gtacattccc ttttcttagt ggtttttgta ccgcatgttt ttgtcctgca actgtccggg
     2941 ttttcatcga acttgttttt gttacgggag taagtgcctc cttccgacga cgacttgttt
     3001 gtccctgacg acgttttcga tcgttcaatt ctgttctagg cgtatgcttc tggccataag
     3061 acttgtgttt ttgctttgga ttcgctaatg acaccaccat tgcagaaccc tgattgcgat
     3121 acggactacg agacgcacct tttctacttc tttctcttag actaggattg ccttgcaatt
     3181 ctcgttgtaa taaatcggct gtttttcttg cctgtttctc cattgctgtt cgttgcatac
     3241 gttcttgact ttgctcttct gcttgtttcg cttgatcatg caagtctctt gctttttgtg
     3301 ccatacgttc catacgattt ggtgctttat gacgaggttg ttgtgcggat tcacgccgaa
     3361 aagcacttcg tgtggcttgt ttcgccgttt gtggcatacg aacgtctgag ccacccccac
     3421 gttttcgttg acgttcttgt ctcttccgtg cgttttcttt ttctttttgt ttctgtgctt
     3481 ttctcgcttc tttgcgttct ttcaaccgtt gtttggcttc ccttctcaat tttgcttctg
     3541 ccccaaatgg atttcccgca aaagctaacg ctcctcgaac acccattctt ccagtaaata
     3601 gactgcctaa aaagcctcgg aacaaataaa ttcccaacgg cgcaaacaaa taaacaatga
     3661 tcaagagaat tttttcccat ggatcgtctt gtgtactact taattggaac ccgtaactaa
     3721 gaaaagaaga aaaaaggata catgcaaaac ctgtcattcc ttttaaaaac gtcaaaatac
     3781 caaaaacttt ggcatagtta ccaaacacat tcattgtgac aagaaacaat cctaaaaaaa
     3841 gtaagaacgg caacatcggt actaacaata gttgcatagt tccaataatc aatcgaaaca
     3901 tacttaagac aaaataaaca acggtttgaa tcagattcac gacaaaataa aacaaggcaa
     3961 atgctgtatt tttccaacta ttgtaatatt gtatgttctt gtttttcagt gtatctgctt
     4021 catactctgt gactttgtca tgaattttct cccctatttt tgtacctaaa tcattgtcca
     4081 ataaaagatt gattcgatca tattcttttc cagcatattt gactttcttc tcacgaattg
     4141 tgtctacatt ggttgtgcca taatttaaca acagatatgg ttcataaaca ttactataaa
     4201 agacccgagc cgccatcaat ttgcctgccg tttccacttt tttatccgct gttacctctt
     4261 ctgtttcttt tgtttcataa tttgtttctt cttcaggtgt taacactgga ttgaccttca
     4321 cgaacgccgt ttccactgta gaatctaaag aaaaaagaac atcaaaaaga ttcttatttg
     4381 tcccagaatc ttttaatacg cttaacccag taaaaataag gatcgtcata agaaagacac
     4441 ggagtgcttg tttcattcga gcctctccag catatttgat tgccatcata attgccgaaa
     4501 aactgattcc aatagttcct gcaatactta acatattgtc cgcaattttt tgtgttaagt
     4561 tttgaatggg ttctcgaatg gcggaaataa tatctaattc aaagagaaat tcaaccattg
     4621 aagcattgaa ttctcctaac atttttacgc cactccaaac aaaatttttc atcactcctg
     4681 caacattgta cgatagcttt tttttaaggt tcattgactc atagtcattg gtcatcaatt
     4741 caaacgaatt ctttttgtaa tttttataca attgatccac atcagtcgtt acttctttta
     4801 cttctacttt atcgttggca ttctctcctt cagaacgatc ggtttcagtg acacccgcac
     4861 catcattcat ttgatcggca gaaacagtag ccccttgtag acagaaaaaa agacccccta
     4921 ataaaaggag cgtccaccat ttcagagatt tttttcgcaa aaaaaccacc tcttttttgt
     4981 tttatcgttg tttttcattt tctaacattg cttgaatttt ttcatcttga ttttcagtag
     5041 aagaaacagc caataaccaa ctatcaaaca acacatcaat ggcaatcggt tgatttcttc
     5101 ccaaatgatc ctggaataaa caggttcctg tttgtaattg ttccatcatt tctaaattgg
     5161 ctgaattcac ttccatatca aagaaggaca atagtttctc tcgtgcttct ttttgtttcg
     5221 gacgaaacgc aaatttgtaa gaaattaact cttttttatc ttcttgatcg taatctttaa
     5281 acgcttgcac aacaaggtaa atatccgtat tattcgcacg tccttttcgc aagctatcat
     5341 caatcaaata gcttccttgt gcggtatctt catacccttt tgcctcgtca taaataatcg
     5401 cagcgtcttc ttctttgttt gtagaaaaga tatacgtaaa cttcataatg acttccataa
     5461 ttgccattcc tgcaagttgt tcgctattta atcgacccga atcaatctct ttttgtgtcg
     5521 gaatgcgtat tccttgtgtc cctaacacgt tgatttgtga atcaaaacgt atcggcgtac
     5581 tgtgctcatt cccaatgaga atctttccta aaccaacgct atgccctaaa atcaatttag
     5641 ctagttcttt gtcacttttt cgaatttctt cgataacttg acttaacgaa tgcttacctg
     5701 gcatggtgta aactacatcg atggcgtctt ggatcaccgt tttcttgtgg ctcgctgttt
     5761 ctcgattggt atctacttct cctaacattc ctaacacatt tttagcggtt tgaattgcct
     5821 cttcttttgg taaaaacaat agaggatcaa gcatccctcg acagctttca tctgaagata
     5881 acgaaataaa attaattcgt tcatacatag ccacaaactc tggtatgtgt ccgaattttt
     5941 caagtgcctt taaaaagaac ttttgggttt catttttcgg atcaatataa agaatttttt
     6001 gacctaaaaa catcagccat aaaaaaatat attttacaag gaccgatttt cctttacctg
     6061 gtggaccaat aatcaacgta tttccattgg tatgttccgc gccttcaata cttttctttg
     6121 ttaaattggg ataataccat actagtttac tagatagcgc taacgcttct ttcgcagatt
     6181 taaacttttt tgcaataaca atccgtccta aaggaaatcc tttcttattt cctacttctt
     6241 tctttaagtc caagccataa tctgccacaa aacctgtcgt agcgacctgc tcgtaactac
     6301 gaaaacgttg cgtacttcca attaagcatt gattaaacaa cgttagctga tccgctaccg
     6361 atcgataaaa tttgaaatct gtagactcaa tcgcaaattc aatatctttg actcgttctt
     6421 ccaattcttc ttttgaagaa gcagatacaa ccagcgtgac ggtagaataa cacaatcgtt
     6481 tttctttgcg cttcagctta tcttttacat ccattaaccg ttctgtacca aaatttaata
     6541 catcgtctga atctaaaatg ccgtcaacgg tctcatattc tttttcttgt tcataaatcc
     6601 gtccagccat tttattaatt tttcgttcat cagattttga atgctcaaat cgaagtcgaa
     6661 tatgcgtatc aattggaaaa gataagctat cctgaagatt ttgaataaag ctactcccat
     6721 acatggctaa aggagcgtcc gtttgactta aaaacgtcga ataatgcgtt tgattcaact
     6781 gttcaatcgc aatgtatcct gcttcattcg aaatgacgcc ctcgcaaatt tccattggcg
     6841 taagctgtct ttcaggcaat ttttcttctg atcgatggaa aaaataataa taaatttgtc
     6901 caatctcttg ggcagataag cgctctatcg gcttataatt ggacaattcc gtatagagct
     6961 ccttttctaa tgtttcatac gttggtaaca cactacttat aggaactcgt tgtcccgtaa
     7021 tacgatagat aaacttttta aaaacatgct taagcaattg aaaatattcg atcggatcag
     7081 acactttcat ttctgtagtg aaacgcacaa acagatacgt tcgatactcg tttaattgca
     7141 cttcgtcttt taaaatcttt cctgcctgtt caaagtacac tttcccaaga tctgcgcatt
     7201 ctccctttac caaatctcga atggtttctt caatgtgcgt atctaggtta aattgacgtg
     7261 gaatattgat aaaatgatag tcatattcat catgtccaag tatgccttcg ccatcttgaa
     7321 gatagttctt gaaaaaatca atgtcattta aaggaaatac ttgggaagaa agttgatagc
     7381 ctacccacac atcatcattt ttggtcaaaa caaaatttcg gataatggcg tctatcggaa
     7441 attctaatcg aggaagcttc gtttttttct ttttgaactg ccaaatcccg ataataaaga
     7501 cgaccaaaag aaacaaaaac gccatttact cgactcactc cttccatttg tgtagggaat
     7561 acgtcacttt ttcatggaaa aacggcacat acatttcatg ttcaaagcgt tgtttccgtt
     7621 tctgataata ggacgttcgc ccaattaaat actcgaaaaa gtgtttattg tcccatttca
     7681 ttgaaaacaa tgtccaaacg agaacaccaa tcccaaaaat cataatcaac caggactttc
     7741 gaaacagtgt ctgcaagaaa ctaatttgat tcacatacgc aatcaaaaat aggatcaaaa
     7801 gaatgaagag aaatacccca cctatgacta attgttccat gcgaatgcca ttagaaatcc
     7861 agggaatttt ttgtccttta atcccgtaaa ttttgtgagg gtgcttaaac tcctttgtat
     7921 agttgtattc catgttcact ctacctcctt ttacaaaaaa agaagcgacc agaagttcta
     7981 atcttctgat cgcttttgca gtttgtcttt ttcttatgat aattgtatcc agggcacaat
     8041 catttgcgtt acccccacgt tctcttcact atccttaata aagataatgt tctttagcca
     8101 ttcaccaata tcaacggcat tcgataacaa taagacgagc attatcaaaa taaagacgcc
     8161 aataatggca aacgctgccc gcgcaaagcc ttgggtcgca taagcaccaa ccattagaac
     8221 aatcgcaacg attgccaata gccaaatgac ttgtccagat acatcatcaa ataacgattt
     8281 aagatcaaaa agtttcattt ccattctcct ctaagttcat tttattcatt taacgtagta
     8341 acaaaataac tatcgccact tgtttttact tccattgaaa agtttgattg gaactcgttt
     8401 cctttctcaa aatagaaacg atacttccct tgcaaaaagt agtttccttc tttatttttt
     8461 actgcatgat ccagttgtac attgatcaat tcgccgcctt ctaacccttt ttcgttagaa
     8521 atcaatgcca atttttcatc atttttgaca tataactcaa aaaaccgttt cacaaatgct
     8581 tccagcttga tttcttcgct ttcagtgacc gcttgcccag aagaataaaa tcgattttcc
     8641 tgatagttca cagaattggt attcttactc tcagaaagat tcaaaaattg aacgccatca
     8701 agtactttta aaacattgtt ttgataactg actggaaaag aaagttgact gcttttccaa
     8761 tgttctcctt ctgtaaaagt aacctcataa aataggcgat acattgcttg tgtcaaatcg
     8821 atttcttcta gtttaataaa gcgaacatct ttcgctactc gattggtagc cgtcgttaca
     8881 aagcctagat cagacgcttc caatccttgt gctaaataat gattgacgga agcctcccat
     8941 tcgtttcgtt tttctggcga ataagtaaat aacctttcaa taaaatgttg tccctcgtac
     9001 ttcaattgtt ccgtagaaga caaggtttgg ttgacttcag ttaacaatgt ttctggattg
     9061 acttgttttt tcactgctaa acgcgttaat gtatccactc gtccaaagtt taggaggagc
     9121 acgacaccca ttaacagaaa caaacaaaaa aaagcgcctg aaaatacact catggcactt
     9181 ttttgcgaaa attggatcat tcgatctgac ggttctgttt tctttttttg gaacaaaaaa
     9241 cgtttctttt gtttcttttc cttctgttgt ttcggtggct tagtttggaa cgcttgtgga
     9301 ttaaattttg cattactcat gagaaatgcc ataccgttct aacaataatt tcggtatttt
     9361 gtccacaatc actacttcgt ggacaaccac acttgccccc tc
//
LOCUS       DADOOS010000107         9359 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.107, whole genome shotgun sequence.
ACCESSION   DADOOS010000107 DADOOS010000000
VERSION     DADOOS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.107"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(90..758)
                     /locus_tag="J8C37_002148"
     CDS             complement(90..758)
                     /locus_tag="J8C37_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ5305570.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            892..1179
                     /locus_tag="J8C37_002149"
     CDS             892..1179
                     /locus_tag="J8C37_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305571.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1595..3133
                     /locus_tag="J8C37_002150"
     CDS             1595..3133
                     /locus_tag="J8C37_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAZ5305572.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3130..4095
                     /locus_tag="J8C37_002151"
     CDS             3130..4095
                     /locus_tag="J8C37_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305573.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4082..5404
                     /locus_tag="J8C37_002152"
     CDS             4082..5404
                     /locus_tag="J8C37_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ5305574.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5467..5571
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5645..6388
                     /locus_tag="J8C37_002153"
     CDS             5645..6388
                     /locus_tag="J8C37_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5305575.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6403..7395
                     /locus_tag="J8C37_002154"
     CDS             6403..7395
                     /locus_tag="J8C37_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5305576.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7407..8144
                     /locus_tag="J8C37_002155"
     CDS             7407..8144
                     /locus_tag="J8C37_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305577.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8134..8856
                     /locus_tag="J8C37_002156"
     CDS             8134..8856
                     /locus_tag="J8C37_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAZ5305578.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8940..>9359)
                     /locus_tag="J8C37_002157"
     CDS             complement(8940..>9359)
                     /locus_tag="J8C37_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAZ5305579.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2712 t
ORIGIN      
        1 tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt
       61 aaattaaata aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt
      121 ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc
      181 agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga
      241 aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa
      301 agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt
      361 ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct
      421 gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat
      481 aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt
      541 ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa
      601 ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc
      661 agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg
      721 attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa
      781 aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa
      841 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
      901 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
      961 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
     1021 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
     1081 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
     1141 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
     1201 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
     1261 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
     1321 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
     1381 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
     1441 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
     1501 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
     1561 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
     1621 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
     1681 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
     1741 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
     1801 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
     1861 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
     1921 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
     1981 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
     2041 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
     2101 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
     2161 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
     2221 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
     2281 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
     2341 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
     2401 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
     2461 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
     2521 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
     2581 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
     2641 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
     2701 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
     2761 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
     2821 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
     2881 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
     2941 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
     3001 attaagcgga aaaataacgg aggatcaaat gattgatatg aattaccaag tcaatgtcga
     3061 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
     3121 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
     3181 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
     3241 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
     3301 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
     3361 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
     3421 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
     3481 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
     3541 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
     3601 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
     3661 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
     3721 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
     3781 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
     3841 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
     3901 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
     3961 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
     4021 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
     4081 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
     4141 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
     4201 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
     4261 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
     4321 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
     4381 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
     4441 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
     4501 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
     4561 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
     4621 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
     4681 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
     4741 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
     4801 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
     4861 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
     4921 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
     4981 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
     5041 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
     5101 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
     5161 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
     5221 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
     5281 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
     5341 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
     5401 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
     5461 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
     5521 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
     5581 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
     5641 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
     5701 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
     5761 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
     5821 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
     5881 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
     5941 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
     6001 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
     6061 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
     6121 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
     6181 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
     6241 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
     6301 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
     6361 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
     6421 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
     6481 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
     6541 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
     6601 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
     6661 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
     6721 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
     6781 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
     6841 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
     6901 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
     6961 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
     7021 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
     7081 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
     7141 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
     7201 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
     7261 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
     7321 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
     7381 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
     7441 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
     7501 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
     7561 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
     7621 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
     7681 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
     7741 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
     7801 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
     7861 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
     7921 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
     7981 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
     8041 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
     8101 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
     8161 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
     8221 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
     8281 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
     8341 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
     8401 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
     8461 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
     8521 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
     8581 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
     8641 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
     8701 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
     8761 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
     8821 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
     8881 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
     8941 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
     9001 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
     9061 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
     9121 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
     9181 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
     9241 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
     9301 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DADOOS010000108         9048 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.108, whole genome shotgun sequence.
ACCESSION   DADOOS010000108 DADOOS010000000
VERSION     DADOOS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.108"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="J8C37_002158"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="J8C37_002158"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAZ5305580.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="J8C37_002159"
     CDS             2714..2938
                     /locus_tag="J8C37_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305581.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="J8C37_002160"
     CDS             2951..3451
                     /locus_tag="J8C37_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAZ5305582.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="J8C37_002161"
     CDS             3548..5395
                     /locus_tag="J8C37_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAZ5305583.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="J8C37_002162"
     CDS             5398..6294
                     /locus_tag="J8C37_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5305584.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="J8C37_002163"
     CDS             6344..6733
                     /locus_tag="J8C37_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305585.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="J8C37_002164"
     CDS             6720..>9048
                     /locus_tag="J8C37_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ5305586.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DADOOS010000109         9028 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.109, whole genome shotgun sequence.
ACCESSION   DADOOS010000109 DADOOS010000000
VERSION     DADOOS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.109"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            818..1291
                     /locus_tag="J8C37_002165"
     CDS             818..1291
                     /locus_tag="J8C37_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAZ5305587.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1300..1527
                     /locus_tag="J8C37_002166"
     CDS             1300..1527
                     /locus_tag="J8C37_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5305588.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2163..2534
                     /locus_tag="J8C37_002167"
     CDS             2163..2534
                     /locus_tag="J8C37_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305589.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2790..3032)
                     /locus_tag="J8C37_002168"
     CDS             complement(2790..3032)
                     /locus_tag="J8C37_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ5305590.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3064..3966)
                     /locus_tag="J8C37_002169"
     CDS             complement(3064..3966)
                     /locus_tag="J8C37_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ5305591.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3979..4167)
                     /locus_tag="J8C37_002170"
     CDS             complement(3979..4167)
                     /locus_tag="J8C37_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAZ5305592.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4181..4744)
                     /gene="amaP"
                     /locus_tag="J8C37_002171"
     CDS             complement(4181..4744)
                     /gene="amaP"
                     /locus_tag="J8C37_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAZ5305593.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4782..5675)
                     /locus_tag="J8C37_002172"
     CDS             complement(4782..5675)
                     /locus_tag="J8C37_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5305594.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5753..6691)
                     /locus_tag="J8C37_002173"
     CDS             complement(5753..6691)
                     /locus_tag="J8C37_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAZ5305595.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6725..7075)
                     /locus_tag="J8C37_002174"
     CDS             complement(6725..7075)
                     /locus_tag="J8C37_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ5305596.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7108..8010)
                     /locus_tag="J8C37_002175"
     CDS             complement(7108..8010)
                     /locus_tag="J8C37_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAZ5305597.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8003..8860)
                     /locus_tag="J8C37_002176"
     CDS             complement(8003..8860)
                     /locus_tag="J8C37_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAZ5305598.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2524 a         1720 c         1494 g         3290 t
ORIGIN      
        1 tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt tgattgtatc
       61 aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta ctacattatg
      121 ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa aaaaataaac
      181 atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta ttcggcaatc
      241 cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata aactaatcca
      301 atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc atgcgtcaat
      361 gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa gtggtgatta
      421 caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact tgaaaaaatt
      481 aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg ttaacttaca
      541 cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata atcccaggca
      601 tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt tttaaatgtt
      661 tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta tcatttttta
      721 taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat aattattctg
      781 ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa cattgctatt
      841 aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac ttttttatac
      901 tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat tatttgtttc
      961 tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat tgaaactatt
     1021 gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac taaaaaaaat
     1081 cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta aatttatgaa
     1141 agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg tcgtattcat
     1201 tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt
     1261 acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat cgttaattta
     1321 gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt
     1381 acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg tttttccact
     1441 ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac ctataaaaat
     1501 gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa taaaaaataa
     1561 tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt cagatttttt
     1621 atttcgattg aataccgttt tatctataaa taagatatgt actgactcat acaaaggggg
     1681 ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg ttacgttgta
     1741 gttggatctg cttttagtat aatactttat tcattccacc agactgatag cagtttgagc
     1801 agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa gtgcttaaaa
     1861 cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta agctcccata
     1921 tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta ctatgtagtt
     1981 ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta acattatttt
     2041 gataataatg attatttatt taaaaataca ttagataaca tttgttacga cacgaaaaag
     2101 cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt aggaggaata
     2161 gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt ccattaccaa
     2221 catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct gctcttacga
     2281 aactttgttt acttctattt gtttgtctag attactttac gagctattat tggctaaaac
     2341 agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc tttgagtttc
     2401 tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc atctgggttt
     2461 tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa tttagggagg
     2521 cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg aaaaactttt
     2581 aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag tcttgtcaca
     2641 gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata tgcccaacca
     2701 aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gaagaacagc
     2761 tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa tgatacgatt
     2821 agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc aatacttggt
     2881 ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc tacgacattt
     2941 gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc tccgataatt
     3001 cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg ttttacttcc
     3061 ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc ttccagctgc
     3121 tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt
     3181 tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt tgatgtccac
     3241 tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt ctttgccgat
     3301 tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca caatgacatc
     3361 catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg ttacattatc
     3421 tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag ataataaacc
     3481 gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt cataattcag
     3541 ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat ttgtatgatc
     3601 agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc
     3661 atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg catcatgttg
     3721 ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt ctgcatcatg
     3781 tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat gtgcagatac
     3841 tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg tcgttgggtc
     3901 tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt tgttattgtt
     3961 attcatggat gtttcctcct aagatttaaa atagtgatct aagattttcg tttgtttcag
     4021 ataaaaaccg atagcgacgc cgatcgctgc catgatcaag atcaataagg tcttgaaaaa
     4081 gcccacggtg atccataaaa cggctaatat caatccaagt accccaaaga tgatagggag
     4141 cttgtatgtc gaaaatagtt ctttcatttt tggtcccccc ctattctact cgtttgcggt
     4201 cattgcttcg tttagctggt tcttcaaagc ctgtgtattt caccgatact ttcactggct
     4261 cttttgttcc caatatgtgt tgtacttcat tttcgatttc tttcatcaat gttcctgttt
     4321 taccaatgag tgctgaagtt cgtttcaact ccccttttac attgacggcg atcttgtttt
     4381 tcgttgcttt gacatttact tttggtgaat ggatgaattc atcttcatgc aagtgagaac
     4441 gaaccattcc ttcaattgct tttttatcca acgtcaactc cccatcacca tgtttcagta
     4501 gaaagtttcc gattgttttt ggataaaaca ggaaaacaag taacgtgata attgtcaaaa
     4561 tagcaaatgc aacggcaagc caaaatacat attgttgcac gtatgaatta gtgatggaaa
     4621 agaatcgaaa cttctctagc tgaaaaggca atggataggt tgcacttaat agtatcgcaa
     4681 acaaaagcac agatagcaaa agtaatgttc cgatcactcc gatcgttttt acacctttat
     4741 tcattttcgc acctcctcat aggggtgaaa cgatcaactt cttataagtt gatcgcttct
     4801 ccaccagtta ctccgtaaat ttgggctgta acataacttg ctttattcga tgctaagaaa
     4861 acataaactg gtgctaattc tcctggctga cctgcacgtt ctagtaaact ttgctgacca
     4921 aattcaggga tcgaatcaga aggttgtcct tcgtctagtt gtaatggtgt ccagattgga
     4981 ccaggtgcta caccatttac acggattcct ttaggtgcaa gctgttttgc taaaccgatc
     5041 gtaaagttag cgattgaagc ttttgttgct gcgtaatcta ataaatgttc gcttggattg
     5101 aatgcttgaa cagaagttgt tgttacgatt gctcctcctg ctgggatatg cggaacagct
     5161 tctttcacta aagcaaacat agcgatgatg tttatcataa atgtgtcttt gacttgctca
     5221 agtggtaact cagcaataga ctgacaagaa atttgctgtc ctgcgtttag taccatcgta
     5281 tctagcccgc caagttcact cacggcttta tctatgattt ctttaggtgc ttgttcgtca
     5341 cgaagatcaa aaggtagtaa gacaatctta cgtcctgcaa ctttcacgta ttttgctact
     5401 tcctctgcat ctttttcttc tcctgggaag aattgaagag cgatatctgc cccttctcta
     5461 gcaaatgcaa tcgctgctgc acgaccgatc cctgaatctc caccagtgat tagtacacgg
     5521 cggttttcta gttgattatt gccttcatag gattcttctc cacaatcagg aacaggaatc
     5581 atattctgct gtaaagctgg tgtatcctga tcttgtttag gaaatttctc cgtatggtac
     5641 aagtttcgcg ggtcgattaa ttttggttct gtcattgctg tattcctcct agtttttaaa
     5701 ttgtattgcc aaaacaaatt tctttgtttg atacctgttt taaatagatt ctttaatttg
     5761 tataatcttt ccggtataaa tggtaaccta aaactactgc tgctatcaaa gagccaaaga
     5821 ctacgagtat aaagcctaga gcagaacttt tacccggtaa tcctcctgta ttcattcccc
     5881 aaatccctgc gaacaacgcc ggaacggaaa tgattaatgc agctgaatct agatatttca
     5941 tgatatgatt tagattattg tccatcatcc cgttgaatag atcaccaata ctctctaata
     6001 aatctcgata gatctcgatc attttttctg tttgtcgctg tctcaaatgg acatcataga
     6061 gtagctgttc atcgtctaaa cgtttgatga aagtttcaga attccataat tgatccattg
     6121 tttcccgttg atctcttaag gtatgatcga tatatacgat gtctctttca agatctgcct
     6181 gtttatacag ttcttcattt tccgttgttt tacgagctgc tttatccaaa gcgtcaattc
     6241 gctttttcat ctctcttagt tctttcacgt aatgggtgta aatatctaat aatgtatacg
     6301 tgatgatctt ttcaaacgag tttaaatgat ttccatgctt tgtcagcagc cgatccacaa
     6361 attcggtttt tctatcgata caagtaatca agagattatc ggaaagtaca aaagatattg
     6421 gcgttaattt tttttcgata cgattatctg actgatctag attaatcact accaaagaaa
     6481 atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct tccggatatt
     6541 cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc tcaatctctt
     6601 cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt tctattacat
     6661 tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga ctttctcgaa
     6721 actattagct gaagaagcag ttctttaatt tgatattata atcgagtact gcgatttcat
     6781 tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat
     6841 aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt ttagtcgcat
     6901 attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac
     6961 ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt tttgtatttt
     7021 ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt tccattccta
     7081 ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt ttggttgaac
     7141 ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg tcatccctgc
     7201 atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg
     7261 tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac gttcacctgc
     7321 tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc aacgcggatg
     7381 aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat ggaatcgtaa
     7441 tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt catctaacca
     7501 ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt tagctgaaat
     7561 tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta cgattgaatc
     7621 accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt cgcaatccat
     7681 atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata
     7741 agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt tattgatttt
     7801 caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta aaagctgttt
     7861 actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag acgggttgca
     7921 attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg tgttacaact
     7981 tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt agagtactgt
     8041 gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta ttccaaggtt
     8101 tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta tactgacgtt
     8161 catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc atgttccatt
     8221 ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct tggtcgtgat
     8281 agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta atttcttttt
     8341 tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag tcatcggaat
     8401 cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg ccgattgttt
     8461 ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac acatctgaat
     8521 ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa gaaattctta
     8581 agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata tctttttcca
     8641 ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct tccaatcctt
     8701 ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat
     8761 tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa ttttcgtctg
     8821 aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact cctttttcta
     8881 atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa agcaaaatat
     8941 atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat cggtaagcgc
     9001 taactattcg tttttcatgg ctttttat
//
LOCUS       DADOOS010000110         8970 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.110, whole genome shotgun sequence.
ACCESSION   DADOOS010000110 DADOOS010000000
VERSION     DADOOS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.110"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(157..1209)
                     /locus_tag="J8C37_002177"
     CDS             complement(157..1209)
                     /locus_tag="J8C37_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5305599.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1222..2160)
                     /locus_tag="J8C37_002178"
     CDS             complement(1222..2160)
                     /locus_tag="J8C37_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAZ5305600.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2611..3261)
                     /locus_tag="J8C37_002179"
     CDS             complement(2611..3261)
                     /locus_tag="J8C37_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305601.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3361..3870)
                     /locus_tag="J8C37_002180"
     CDS             complement(3361..3870)
                     /locus_tag="J8C37_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAZ5305602.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4071..4940)
                     /locus_tag="J8C37_002181"
     CDS             complement(4071..4940)
                     /locus_tag="J8C37_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5305603.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(4960..6081)
                     /locus_tag="J8C37_002182"
     CDS             complement(4960..6081)
                     /locus_tag="J8C37_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAZ5305604.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(6083..6478)
                     /locus_tag="J8C37_002183"
     CDS             complement(6083..6478)
                     /locus_tag="J8C37_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ5305605.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(6497..7294)
                     /locus_tag="J8C37_002184"
     CDS             complement(6497..7294)
                     /locus_tag="J8C37_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5305606.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(7296..8084)
                     /locus_tag="J8C37_002185"
     CDS             complement(7296..8084)
                     /locus_tag="J8C37_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5305607.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(8098..8574)
                     /locus_tag="J8C37_002186"
     CDS             complement(8098..8574)
                     /locus_tag="J8C37_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305608.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(8646..>8970)
                     /locus_tag="J8C37_002187"
     CDS             complement(8646..>8970)
                     /locus_tag="J8C37_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5305609.1"
                     /translation="LMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHI
                     SCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYD
                     YVKRA"
BASE COUNT         2821 a         1929 c         1300 g         2920 t
ORIGIN      
        1 aggtgagttt cttctacatt ttaaaaaatc gaatggcact gaagagttca gttgtcagca
       61 tcaatctcct actcaaaaag gcaaaaaaac gtaagtcaga tgttgttgac atctcactta
      121 cgcatccatt gattgaaaat ttcttagtaa aatctactag cctaaaaatt tgaagacttc
      181 ttctcgatca tacagtttaa atactttctc gcataaaaat tgagtaatta gtgccgcaaa
      241 aacagggatg actaaccaac aaagaacaac aagaaagcca tttagccctg catctaaaga
      301 agccaacgga ccaacaagtc cgaccacgcc gaaaccagca gaagcaggcg tacctgaaat
      361 agaaaataga gcgacaggaa tagctgtaat cactgcacta actaaacaag gaataagaat
      421 cacaggtttt ctaaatagat taggcatcat catcttcata gcgcctaaag caatcgcaat
      481 tgtcacacct gacttattaa ccttccaaga gttcactact aaaacaaaag tggttgcagc
      541 tacgcccatc gcagccgcac cagcagaaac accatctagt tggattgcca ttccgattgc
      601 tactgttgaa ataggagaga taatcaaaaa ggcaaaggaa caaccaatca agatagacat
      661 taacagcggt tgtaaggtag tgaagttatt aatcaatgat ccaattgcag aagtaattgc
      721 tgttacaaaa gggaataaat aaaaaccaag taaacctgca cctacaccta caacaatcgg
      781 tgttaacaca atcgacactg acccaaaact atttccaatc agtaaaatca gcccgacagc
      841 aatcgatgct gtcagcatcg tattaataat atcgcctgta ccagcaccaa cgtaaacacc
      901 cgtttcaccc aaatcaggtg tgaatttgac tacaccagaa ccaacaaaag aagctccggc
      961 tacaaccatc attctcatcg gatcaaatcc aaattgtaag gcaatcaaac caccaatgat
     1021 caaaggtgtt gctagttgaa aaataactgc taactgtgcg atcgttaccg caaagggata
     1081 cgcactaaaa tattttaaaa tggcacctaa tactgcatta ggaatcaatc caataatgat
     1141 tcctgttgct gtaccagcga ggactttatt cagaaaaatt ttcggtgata ttttttgtga
     1201 agtttccatt caatctctcc tttattttac gttcaaacta tcttcttttg catggacaag
     1261 ctcagtcaag aaatcacaat aaggcgtcgc aacaccatat ttcttccctt ttctagaaat
     1321 tgcgccattg atataatcga tttctgttaa tcgatggttc ttaatcaagt cttgatacat
     1381 tgaaggataa tgtaacccaa ttgtttccgg gtcaaaacaa gattcacaat gtgcaatgac
     1441 ttctgggaca tctagttcaa tcttctctac tgctgctact ttggcaaact cattgacaat
     1501 cgtcgccacc attttatgag cagtcgatgt ttttcctagc tcagccatat taacgtctaa
     1561 gattgtgcat agtccgttca ttgttccgtt cacacatgct ttgcgataaa tagaatagtg
     1621 aatattgtca gaaaaatgag catttaatcc tgattcagac aatttgtctg ctaatttttt
     1681 tgcagctgct tcttttccat cacctaaatt ttgtaattct actgaaccac ttccaaataa
     1741 tttgacctga ccaggacctt caagacctgc cgtccacatg gtatttccaa tatagatatt
     1801 ttccattggt acaaatttct caataatatc ttcatgcccg ataccattta atagacataa
     1861 gacctctgta tcttttttga ttaatgattg gatatcctgc agcatttttt ccagctgcat
     1921 cgctttggta aatagaataa tcagatcaac ttgatcttct ttttctactt cggattgaag
     1981 aacgattggt agttttgctt ctacctcttt accattaaaa tttgcttgta atccatgttc
     2041 tttgatttgt tggacatgtt ctgcccagcc atcgattaat aatacttcgt tcccactttg
     2101 gtgaagcatc aacccaaagc gacttcccat tgcgccagcg cctgcaattg ctattttcat
     2161 tgattcttcc tcctttttct ttctattata ctcatttgag aatcttttca caacctcgtt
     2221 ttgttattct tctcttattt atcatgttta tatccaccat tcaagttatt ttatcagcaa
     2281 atcaataaaa actggaatag aatcccaatt tcatcgtaag agaaactaga ctagcaataa
     2341 gaaaatagaa acattcatag aaaaaatctc actcctctga ttataaaaga gggggcatta
     2401 acctcgtgta tactaaaacc ttccattcat tcgatggatg attttctttt tgtgaaaaaa
     2461 tattcattgt agtacgatgt tgcgcaacac agacggatgg tattgcttat tatttattaa
     2521 aatcgaacaa tacacttatt taaaaacttc tttcccagat tttcttccat ttttacagtt
     2581 ataactagta ttatcatgaa aaatagcctt ttattgtcta tgggaataat aatttaaaat
     2641 tggtactttt ttattaatta gttcatcgcc caataatctc tccagtttct tagcatctcc
     2701 tgactcaacc ggaattatcc aagcccagtc taaataattt tttaaaatct catcatattt
     2761 tgcaaatatt tcattgccat attccgtcct taaaattata ttttcagtgc cagacaagtg
     2821 atttatattg agttgataaa aaagatctcc atctaaaaaa atatcattag aagaagattt
     2881 ccccacagta atgaccattc catcagctct atgtgttaca tacaaataat tgtatgacct
     2941 catttcacct aatgaatcaa ttatttcttg ccctttagct ctaatatatt gatctctatt
     3001 acctatctca ctcttattta acaaaagttt tttcatgtca aatttaatcg gctgttttat
     3061 ttttgtaaat tcgcttctac tactaatcca atccgagatt aatttttcaa catgtcccag
     3121 agtaaactct gatttattcg ctaaatactt tttttgttgt attaaagaca tctttccccg
     3181 aagctcaaaa ttgagcctac atcgattctc tatctcttca aaaagcatct tatcttcatc
     3241 attcttatat tcatcattca taaaaccaat ccagctcctc gattaagata atattttaat
     3301 ctcatacaaa aactagctct catcaaaaca accattgttt tgatttctca tgaaatttct
     3361 ttatactttt ctccgattat aaattcctaa ccccttgaga aaattacgca tatatttatc
     3421 tccgcaaggc ttaaaatttc gttggccttc ttttctcaaa aatgccccga tttcgccttt
     3481 tgatggatag atttctgcct cagcaaaaac gtctaaaata tcatcagtgg tcatagacaa
     3541 ggcgattttt aattttttta gcaacacatt gttgatatat ttgatttcct tgcttttttg
     3601 cggcgtgtct actatcggct tatctttttg cggaccacgc gccaagagaa tcaggccatt
     3661 taggaatgcg tcaaaaactt ggttgttgat ggttttttca tacacattat tttctgcatt
     3721 atctttgttc ttatcctgta ctttggtcag taccgcctga gcttcttctt tagtcagtgg
     3781 tacaccgcct agctcaaaag ctttaatcaa atccgtatct ttcaaatcaa tcgcataacg
     3841 caatcgcaat aacctatcat tgttattcat ttgttcctcc gaagtattta gtctttactt
     3901 tcttacgtac tttttgtctt gctaagattt ttaggatcaa tgataaaaac cgcatcactt
     3961 atgataaggc gctgggtatc atgagtgaat gatattgctg aatacaaata gtccgaccac
     4021 attaagttga tcggacttca taagcatttt attgactaat acttttttgc ttaagaattg
     4081 attctaacat cttttgctaa agttaagcat cctatagttc ctgcattatc tgctaactta
     4141 ggtgtgacaa tgtattcttc gatatttggc gtatcgacat agccgttaat tagttctaaa
     4201 aaacgtcttt gtactttctt tttcagatgt tcttgttgca tgacaccacc accaaaaatt
     4261 ataatatctg gtgaaagcat cagggtcgta ttacaggcac attgtgcaat ataatccgcc
     4321 tcgatttccc agaacggatc ttctatagaa agatcttgtc ccttggttcc cgtacgcatc
     4381 tcgatagcgg gtccagatgc caacccttcg agacaatcat gatgaaaagg acaagtacca
     4441 gagaaagtat ctcctgaatg tctcttaact aacgtatgcc ccatttctgg atggctaaac
     4501 ccttcaacaa aaatcccatc ttgtatagct cctccaccaa ttccagttcc aacagtgtaa
     4561 taaacaacac tggataaacc ttttccatga cctgaaacat actccccgta acaagcagca
     4621 ttcacatcgg ttgtccaggc aattggaatt tcgaaatgct tattcaacgt tccaacaaaa
     4681 tcaaaatttt tccaggcagt ttttggagta gaggtgatat gtccataggt ctttgattca
     4741 cgatggatat caatcggtcc aaatgaccct actccaatgc tttctaattg ttctttatag
     4801 tgattaaaga aatcaattac caaactcatt gtttcattcg gtgtagttgt aggaaaactc
     4861 actctttcta aaacctttaa atcatcattg ccaatggcac aaacaaattt agtcccacct
     4921 gcttctattg agccatacat ttcttactcc accgtccttt tatctaggaa ttcgttcttc
     4981 cctgtctaat agtttttcga aaaactcatg tggttccttt tggtaccaat aggcaacaga
     5041 agaaatatcg tcttgacgct cataaagctt gatgtcatca tttcctaggg tttggaaagt
     5101 aacacgtaaa ttttctttga aactaatggg gtcgggtaaa tgccagcggt acaacccatg
     5161 catcggtaaa gcatcatcgc caaaaccatg aactggattt ggattaggtt ttccagcact
     5221 aaaataatcc cgggtgtgat ctcttttaga ttgaaaagga tagcccataa atagcgtatt
     5281 aaaattttga gcatatgccc tttctccaaa gttcttttta tggaaagccc aagcgccgcc
     5341 aaaataatct tctgctcctg tagatgttat tgtagggaaa tcttcatctc catctatata
     5401 aaacttaaat tctccttccc cccaccagta tctttccaat gcgcacaaag caaagtaggt
     5461 tcctacataa tacccagtgc ctttgattcc atcaataagg gtgtaatctt ttccttttgt
     5521 cgtgatgggt tcacggttcc atttagcatg aaaatacatc gcatcatccg gtacttcagt
     5581 caatgcataa ttaaatgtat aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa
     5641 agtaatctta gctttttttc gaaatggcat ctcaaaatag caattcatcc ctgatgttgg
     5701 attcactacg attggtaagg aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa
     5761 atctcctaat ggactttcta cagaaggagt ggattcatca tcccaataaa tacgcaaaat
     5821 taaatcacgt aagacgaaac tcccagcgat tgttttgtct gtcactgtca tccaaaaatg
     5881 acgtaaaatt ccagggcctt ccatttctgc taaagtaatt gtctctccag catttatcgg
     5941 aacagaaggt gacccttttc tcccaattcc tagatgactc gctgtcattg ccgctttccc
     6001 tttttcacca tttttatttt ctggtgaaat agttctactt tcctctccct tgtggagcca
     6061 tatattattt aaacaattca ttttattcgt cctctatcct ttcttcactt ttttgattat
     6121 tcatatagat gatttgttgt tgtgcctccg caataattgc tccaatatct aaattatcat
     6181 ttcctaactg caaagcgatt gacattgcca agggaagatt cattcctgca attacgtgca
     6241 ctctgtcaga aatataggta aaaaactttt gatttacact tcctccagcc atgtcagtta
     6301 aaacaattat ttcgtctgta gtatccacta actctttaat ttgttcatct atatcatcaa
     6361 tttcttcgac atatgcacat aagctactaa tttgattatg catacctgtt aaaaactcta
     6421 gtgtctcttt aaaaccctca gctagtattg aatggctagc aatgatcaat cttctcataa
     6481 ttcaaatcct tttcctttac gctaatattc caaatgcagc cataaccatc gcaaaaacaa
     6541 taacgagtag aatcaaaatg gtcatactga tttttttctt actgagtaga taatatgctg
     6601 cagcagtaat aattactgga attaatgttg gtaaaatttt gtccaacatt ggttggattt
     6661 tcatactaac ttcaccaaaa gtgaatttaa gtggcgttga aacactgata actgaagcaa
     6721 ttaatgatcc aactactgtt agccccaata ctgaagcagc ttcagttaaa taattaatct
     6781 tttcaccgta ttcagtaaca atcttcattc cctgcttata accaaattca aagcctctca
     6841 ttcggaaaaa gaagattacc atgattaaaa tgaaccataa aaagattcct acaggattat
     6901 tttctaaagc catataagcc gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa
     6961 gagagtcccc aataccagca attggaccca tcgtaccaac tttaaagtca ttaatcgcat
     7021 cttttgcttc taatccttgc ttttcctcca ttgcaacaca agcacctaaa ataatggctg
     7081 ccagccaagg ctgtgtattg taatactgaa agtgattatt taaagcttcg atgtattcat
     7141 catcatcttt gtataatttt cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt
     7201 gctgtgtttg ataattaaag gtacaaactg acatcaacca acgatagttc gctttcctaa
     7261 gatccttttt cgttacttga tatttttcgc tcattttatt catcctcccc aataaaccca
     7321 acatcagaag ctcccgcaga agcagtgatt tgtgcatctt tttcagctct atgatacaca
     7381 ttaaatgcta aagcgaatcc tatgatcgcc gctccaataa taggtacctt caaaaaagcg
     7441 gctaagaaaa atccaacaag taaataacta atgtatcgac ctgtaggcat aaagcgtaac
     7501 aacatcgcaa taccaacagc aggcaacatt tttccggcta atgttagccc atccataaac
     7561 caattaggta aaacatccaa aacgtaatta acagcagact gtcctaaagt aacggcaata
     7621 aatactggaa ttgctgcaga taaaccaaag aaaattggcc ccaaccacaa aacactattc
     7681 attttcttaa attctcgttt attcgcatat tcctgtgatt tgcgaacaac aaaaccattt
     7741 aagattttga caattacatc tagttgcacc cctaacatac ctacaggtag tcctattgct
     7801 aatccggctt ctactccaag ctggctacca aatgccgttg caataattgc gcacgttcca
     7861 tagtccggta cagatgagcc accaaaaccg gcaactccta atgacatcaa ttgaactgta
     7921 ccgccaacta tcaaacctgt ttttatatca cccatcaaaa ttcctgtgaa taatccccaa
     7981 aaaactgcgt aatttacaaa gatcatcgga atcccataat aatcaacatg tttaataaat
     8041 gctaatatag tcagcataat aatttgaaaa atagtaaaat gcattatcct tactccttta
     8101 tcctaatttt gattgaattt cctgtgcctt atccgccgga acaaattgtg taacaatttc
     8161 tacaccagaa tccaacaatt ttttatacgt gctgatattt tcatctgatg cataggctct
     8221 agaagatagc tgtaatgcat tatctttaac aacagtaccg ccaacaatta gtcggtcaat
     8281 tctaataccg ttatctacta aatgaaggat tgtttccggc tctttcacga tgacaaatac
     8341 tttttcctga tcgtatttcc cctttttaaa attcgcaatc gctgtttctt cagtgataat
     8401 tgatacagcc attccagcag gtctagctaa acgcatactt gatttttgga tgtcgttatt
     8461 tgcgacagaa tcatcgatca ccatcactct gtttgcttta gacattggtg cccattgcgt
     8521 gacaataatc ccatgcaata aacggttatc aactctcgac ataacaatac tcataaatac
     8581 ttcctccttt atctttcatt ttcttacaag caattaccat gccaaaacaa aaagaacttg
     8641 ataaatcaag ctctttttac ataatcataa acgtatttaa cctcatgaat cgggatgtcg
     8701 acactataat aatgttcaac gacactgaaa gattgcttaa aatgtgtctg aaatttttga
     8761 aattcctcac tcgtttcatt gaaataaatt tcatcttcca aagtattttg ttttactaat
     8821 cgttcaatta agcaactaat gtgaacatat aatccgaaac acgtattatt atcaaatgaa
     8881 attcctagat cattttgtaa gatgtatata gcatctgaaa cccgttctaa taatttagtc
     8941 ggatttaata tggtcaattg ctccattaaa
//
LOCUS       DADOOS010000111         8757 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.111, whole genome shotgun sequence.
ACCESSION   DADOOS010000111 DADOOS010000000
VERSION     DADOOS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.111"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            26..535
                     /locus_tag="J8C37_002188"
     CDS             26..535
                     /locus_tag="J8C37_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5305610.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            628..1790
                     /locus_tag="J8C37_002189"
     CDS             join(628..904,904..1790)
                     /locus_tag="J8C37_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ5305611.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2070..3050
                     /locus_tag="J8C37_002190"
     CDS             2070..3050
                     /locus_tag="J8C37_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5305612.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3172..4590
                     /locus_tag="J8C37_002191"
     CDS             3172..4590
                     /locus_tag="J8C37_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAZ5305613.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4661..6130
                     /locus_tag="J8C37_002192"
     CDS             4661..6130
                     /locus_tag="J8C37_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5305614.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6140..6607
                     /locus_tag="J8C37_002193"
     CDS             6140..6607
                     /locus_tag="J8C37_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAZ5305615.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6643..7515
                     /locus_tag="J8C37_002194"
     CDS             6643..7515
                     /locus_tag="J8C37_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5305616.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7596..>8757
                     /locus_tag="J8C37_002195"
     CDS             7596..>8757
                     /locus_tag="J8C37_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAZ5305617.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYN"
BASE COUNT         3021 a         1306 c         1776 g         2654 t
ORIGIN      
        1 ttttattgtt tagggctata ctatattggt acaaggaata gaaagaggtg tttatgtggt
       61 tgataaaatt cagtttaatc aagttataga agaattcatc aaagtaaata atttaatgac
      121 ccatattcaa aaacgaccaa tcagaataac taatgatcta aaactcagta cgagtatgat
      181 tcatttgatt gatgtgattg gaaattatcc tgaaactagt attacggaat tagctgatcg
      241 attaggcgtt accaaaggag ctgtatccca acagattccc actttagaga aaatgaatct
      301 tataagcatt tctcaaaaaa aaggaaataa gaaaactaaa cttgtatcgt taacaaaata
      361 tggtcgaaag gtgtacgaat cacataatta tttacatgaa gagctttata gctcaataaa
      421 tgaagcgtta gaatcattct caccagacca aatttcaacc attaaacaga ttctagaaaa
      481 agtatcttat acaatcaatg aatatcaagt aagtctgaca agaaaggaag attgacatga
      541 aaaaaatgat ttttgaatcg gctacactag actagacaga aaaaataagg tgtgtaaaat
      601 aaatgaaaac acactggagg aatttttatg tcaagacgtc aacgtagaac ctattcaaaa
      661 gaattcaaac aacaagtcgt cgatctttat ctcgcaggaa agccccgagc tgaaatcatt
      721 cgagattatg agctgactac ctcggccttt gataagtggg tcaagcaagc gcaaactact
      781 ggctcgtttc aggaaaaaga taacttgatc cctgaacaag tagaactaat tgcattacga
      841 aagaaaaata aacagcttga aatggagaat gacattttaa agcaagcggc gctgatattg
      901 ggacgaaaag acaagtaatt gataccaata agcacaaata ctcaatatca gcgatgtgta
      961 aagtgttaaa catctcttgc caaacctatt attatcaagc caaaccagtt gaagatgaat
     1021 cagaactgga agaactcgtt caagaagaat ttattcgcaa tcgaaaagct tatggtacca
     1081 ggaagctgaa gcagtgcttg gccaatctag gtcttcaagt gagtcaacgc agaatcggcc
     1141 gtatgatgaa acagcgtggc ttgcgttcaa catacacatt ggctcatttc aaattccatt
     1201 cttctacttg taacgaagcc aagaccgcca atattctaga cggaaccttc ctacaggaaa
     1261 agtcattaga agcgattgta accgacctta cctatgttcg tgttgggaag aaatggcagt
     1321 atatctgcct gattcttgat ttgtttaatc gagaaatcat cggctattca tgcggggaga
     1381 agaaagatgc gacgttagtc aaggaagctt ttgcaaagat tccgtactcg ttgaataatg
     1441 tacaactttt tcacacggat cgtggaaaag aattcgataa ccagacgatt gacgaaattt
     1501 tatgggcctt taatatcacg cgttccttaa gcaaaaaagg ctgcccctat gataacgccg
     1561 ttgcagaatc cacctataaa tctgtcaaag tagaattcgt tcatcaatac cgctttgaga
     1621 cattggctaa tttgcgatta gagctatttg attacgtcca ttggtggaat accatccggc
     1681 tgcacggcac gttaggctat gagacaccga tcagttttcg acagcagaga ttggcgcagc
     1741 gaatccttga taatgagctc ggatgtgata ccggtggcga ggcaccttaa ttatttgaat
     1801 gagcttctgc cttagagaat cagattcgag gaggctcgtt gtcaagggca gtcggaacac
     1861 cagcagcaag cttacgcaat accttataat ttttgtcaaa aaaagtgttg ccattccaca
     1921 tatatcttta aacagctatt gccagataaa aataaatgat attatatata taaatactta
     1981 tccgaaaagg ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga
     2041 ttttctatct acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga
     2101 aagaggcaaa tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga
     2161 gtgaggaaac aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg
     2221 aactggcgag agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa
     2281 ttactaatcc atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg
     2341 gatataaagt gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga
     2401 cgatgttgag aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg
     2461 cggattatga tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata
     2521 cagttacagt atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa
     2581 atcatggctg tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga
     2641 atagtaggac tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc
     2701 tagaagtacc atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg
     2761 aaactcatcc aaatattgac ggggtttttg caggagatga tttattggct tctttggcga
     2821 ttaacaactt aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg
     2881 atggagctca acaaacattg atttataatc cagaattatc aactattcaa cagcctatag
     2941 atcaaatatc taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga
     3001 cggatgaact ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg
     3061 cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg
     3121 gttgacatat attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa
     3181 ttattaatta aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga
     3241 gatgttatgc cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga
     3301 aatccagttc cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt
     3361 cattttgaag actttgggga atctctttta agaggggatg acaatgagag agaccagttt
     3421 atctacgctg gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat
     3481 aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat
     3541 gattttgttc attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat
     3601 gatattcgaa actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg
     3661 ctaattttag gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa
     3721 tttacatcaa aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg
     3781 tataccatgt ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat
     3841 tcagaatact cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct
     3901 tggattaagc ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct
     3961 tatgatggaa aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat
     4021 aaaaacaatt atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat
     4081 ttttcacttg gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat
     4141 attgccgatg tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat
     4201 agtggaactt tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt
     4261 tcactgagat tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta
     4321 gatgaggggc gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat
     4381 aaaggactta ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt
     4441 gttgatgaca atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat
     4501 ggttgttttg gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat
     4561 accaagtttt caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa
     4621 tcaaacatga cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc
     4681 cgaagatgta ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat
     4741 tactcgttta cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga
     4801 aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac
     4861 aggtataatt gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc
     4921 cgtttctgaa atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt
     4981 taaagttttc tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt
     5041 tttaggaatt cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc
     5101 tgatcacaat gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt
     5161 tgattattta ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat
     5221 tttaggatta cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt
     5281 cgtcatggga actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt
     5341 tgcgtttcaa gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga
     5401 gaaaattaca aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac
     5461 aattgttctt gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg
     5521 taatgtttta ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt
     5581 tgtttttgca ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg
     5641 tattgaggca aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt
     5701 ttcaattatt gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc
     5761 taaagataga gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc
     5821 ggctatcttt gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg
     5881 tgctggtttt ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt
     5941 gactgtattg ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct
     6001 catagcattc attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat
     6061 ccctgaagct aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca
     6121 agtaaagtga gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc
     6181 acctgcaacc ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg
     6241 aatgatggga caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga
     6301 tggttttgtt tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg
     6361 aattgagata cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga
     6421 tattaaagtt caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt
     6481 tgtagttttt gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca
     6541 agacgaaatt ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct
     6601 tggctaatga taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt
     6661 gaagctggtg gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa
     6721 aaagtcagct ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt
     6781 aatcatcata aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt
     6841 cattgtgaat ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat
     6901 tttgatttta ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat
     6961 gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt
     7021 gtttactata ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt
     7081 gaaggtttta gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac
     7141 ttttctggga catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca
     7201 atcgaaagaa gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa
     7261 atagaagctt attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat
     7321 gtcattatat ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat
     7381 aagtttattg agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg
     7441 acaccaaaat tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt
     7501 aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc
     7561 tttttttcaa ttagtaattg aagggacgcc taaaagtgaa aaaaaaaatg aaagatgaag
     7621 tattagagta tatcaaaaat aatttaaagt attacgattt ttctgctcaa gatattgcaa
     7681 tgaaattttg tattaaaaga aatgtcgctt cccactattt gaatcagtta ttttcagatg
     7741 gaaaattact aaagaatgat tctgttagac cagtaatgtt caagtataat caacaaaagc
     7801 caaaagactg cttttctaag tttattggag cagacatatc tttgaaatcc actatagata
     7861 aatgcaaagc tactgtaatg tatcccccta atggcctccc attaataata aaaggaaaca
     7921 gtggcgttgg aaaaagtttt ttagcaagtt tgatctatca gtatgctctt gatagaaaag
     7981 taattcataa tgacgcaaaa tttgttgttg tgaattgtgc agattatgca aataatccag
     8041 agctattgtc agcagtttta tttggatata aaaaaggagc ctttactggg gctgaaaaag
     8101 atactgcagg gatgctatct tctgctaatg gaggatacct ttttttagat gaggttcaca
     8161 atctaagtgc agaaaaccaa gagaaattgt ttcttcttat ggattctcaa aagtatcgta
     8221 aacttggtga aagtgtgcaa tgggagtatg caaatgttcg ccttatctta gccacaacag
     8281 aagataaaaa tacctctttg ttagcaactt ttagaagacg catcccagct gaaatcactc
     8341 tgccagatta caataataga agtacatctg aaaaaattca attattattt gaatttctta
     8401 aagatgaagc aattacgatt gattcaaaaa tattttgttc gattgaactt tttactgatc
     8461 ttttatcgaa aacatttgaa ggaaatgttg gagaattgag gaatgaaatc aagcttcttt
     8521 gtgcagaagg ttatttagga aataagagaa agtcttcaat ttatttaggg gacaaactag
     8581 acagcggttt ttggatagat ccagaaatta gtatagattt aaaaaaaatg tttcttaaat
     8641 cactttctga aattgatttg attgaacttt ttcaaaatca catttcaatt gaagaaagca
     8701 tgagtcaatt aagagataga atacttaaag agtgtgcagg ttcttctgag tataatt
//
LOCUS       DADOOS010000112         8658 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.112, whole genome shotgun sequence.
ACCESSION   DADOOS010000112 DADOOS010000000
VERSION     DADOOS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.112"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(157..2538)
                     /locus_tag="J8C37_002196"
     CDS             complement(157..2538)
                     /locus_tag="J8C37_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAZ5305618.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2528..3175)
                     /gene="recU"
                     /locus_tag="J8C37_002197"
     CDS             complement(2528..3175)
                     /gene="recU"
                     /locus_tag="J8C37_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAZ5305619.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3239..3778
                     /locus_tag="J8C37_002198"
     CDS             3239..3778
                     /locus_tag="J8C37_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAZ5305620.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3868..4287
                     /gene="gpsB"
                     /locus_tag="J8C37_002199"
     CDS             3868..4287
                     /gene="gpsB"
                     /locus_tag="J8C37_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAZ5305621.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4309..4676
                     /gene="rnpB"
                     /locus_tag="J8C37_002200"
     ncRNA           4309..4676
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="J8C37_002200"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4885..6051
                     /locus_tag="J8C37_002201"
     CDS             4885..6051
                     /locus_tag="J8C37_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAZ5305622.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6097..7593
                     /locus_tag="J8C37_002202"
     CDS             6097..7593
                     /locus_tag="J8C37_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAZ5305623.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7674..8357
                     /locus_tag="J8C37_002203"
     CDS             7674..8357
                     /locus_tag="J8C37_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAZ5305624.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2716 a         1577 c         1838 g         2527 t
ORIGIN      
        1 tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc ttataaaaaa
       61 tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca tagacacaaa
      121 aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag aagctgaggt
      181 gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg gctcactgga
      241 agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag aagaaggaac
      301 cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg aggactcagc
      361 agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg ttgttgagct
      421 tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa ttgcattcga
      481 tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta atccgcttgg
      541 catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct cgcgataaac
      601 atcagaggct acgtgtgacg attctgaagt gacaggtgtc atttttttat tgtaacctgt
      661 ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg gatacacccc
      721 agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc cggtcttacc
      781 ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga tcgtatcttt
      841 taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc catctggctc
      901 ataagtttct tctgttccgt cttggaagac gattttatta acatattgag gtttgtaata
      961 agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag aagctccgta
     1021 ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat ggatcgtact
     1081 gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga cttcattgaa
     1141 taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag taatggtgcc
     1201 catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt tatacggtgc
     1261 gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc cgtaatctgt
     1321 gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt tgttgcctaa
     1381 agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc cggtattggt
     1441 gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat actgatccgt
     1501 gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat aaatctctaa
     1561 accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga cttctttgac
     1621 ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca gaccatccgt
     1681 taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt tttgaagcat
     1741 cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat aaggatcata
     1801 ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag aaagttctga
     1861 taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc cgtttgacat
     1921 atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt gttccaatct
     1981 tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg cactggtgga
     2041 gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca gtccgccaga
     2101 ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga tgtgtttata
     2161 aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta attcattcgc
     2221 agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat cttgtgtata
     2281 tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg catcttttgc
     2341 gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg agagtattcc
     2401 taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc cagaaccttt
     2461 gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc tagagcttct
     2521 agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat aaatagaagt
     2581 caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct attcctagtt
     2641 cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg tcccagtaag
     2701 caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac cataacaaga
     2761 caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga tgggcatgga
     2821 agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag tccaagtaaa
     2881 agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag gcttccttga
     2941 tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg gtaggctttt
     3001 tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg attgcttctt
     3061 caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct tgaatttttt
     3121 cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat accatcttta
     3181 atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg aatctgagat
     3241 ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg gtgtttttaa
     3301 agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac tccatcagtt
     3361 agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg aactatgggc
     3421 tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg gtattttgta
     3481 tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa aacaattagc
     3541 agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat cacctagtca
     3601 gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc tgatctatga
     3661 tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt accaagcaga
     3721 tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta gtgaataatc
     3781 gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag aaatgttatt
     3841 acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc taaagacatt
     3901 ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt tgatgaattc
     3961 ttagacaata tcatcaaaga ttatgaagct tacagcaaag aactccttgc tttacaagaa
     4021 gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg agcagctcaa
     4081 acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt tgatatctta
     4141 aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca acaagcaagt
     4201 gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac aagcttagat
     4261 gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt tttcgggtaa
     4321 tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg tgatgcctgt
     4381 agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg gcaggaaaac
     4441 aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac gaagcggctg
     4501 gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa acaatggtag
     4561 gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc agatagatgg
     4621 ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt atagaaaacc
     4681 gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac aagaaaaaca
     4741 tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac aaacttttgt
     4801 atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc ccacctcctt
     4861 tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct cgtcgcaact
     4921 gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg aatcgaatgc
     4981 caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc tacagctaat
     5041 ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga tgcgtatacg
     5101 tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct tccactagat
     5161 gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac acctgattgc
     5221 caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca ccgtccagct
     5281 tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt gctgaaagac
     5341 catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg ttatcgctta
     5401 gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat gttgacaaat
     5461 tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct ttgtattgaa
     5521 gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc ttgcgaatct
     5581 tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga agaaaaagca
     5641 gattatgata ttgaattaga tattactggt tcagatatca atggacggat gattgaaatt
     5701 gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa gcaacaagca
     5761 gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc gccttacgga
     5821 gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg cgatgtattc
     5881 cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt tgaggaattt
     5941 tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg tacggatcta
     6001 ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata atcttactaa
     6061 ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga gtttttacaa
     6121 gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg ggatacacaa
     6181 acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta tctttacagc
     6241 atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta tttttctgaa
     6301 catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa agaagagtat
     6361 gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc cgtttcttca
     6421 gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt tcaacacgca
     6481 ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa agatgaaaag
     6541 acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga aatccttgat
     6601 aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct ccaagaaaaa
     6661 gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca acagaagaga
     6721 tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg tttagatgac
     6781 actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac gacacgatgg
     6841 aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg tcatggtatg
     6901 tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg tacatcaatg
     6961 ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag tcgcagcttc
     7021 tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga tgatattcct
     7081 tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg tatcgaggca
     7141 gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa aatgattttc
     7201 aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata tgaagagtat
     7261 ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca ttggtcaggt
     7321 gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc acaattactt
     7381 cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga ttattctcca
     7441 atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa accaaatcaa
     7501 ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga ctatatgaaa
     7561 gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga tagctgaaat
     7621 tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa cgaatggata
     7681 aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa acaggacatg
     7741 ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag acggaaccgc
     7801 aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt gatgataaag
     7861 tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc tctttgccaa
     7921 tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc ttttctaaaa
     7981 atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac gctgatcgta
     8041 tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt ggacatgggc
     8101 acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa aattaccgaa
     8161 aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct gataaaatga
     8221 atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt gaattcttag
     8281 aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac aattggatca
     8341 aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga tatttcggat
     8401 cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga gcctgccgtt
     8461 cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga tgaatattgt
     8521 aacttgtgat tcggaattgg caggagaaca ccgtttattc ggaagaatag gactgtgaca
     8581 agacttttgt cacagtcctt ttttcgaatt caacaagtaa aacttttaag aaatattcag
     8641 agagtaaaat taaaaatt
//
LOCUS       DADOOS010000113         8589 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.113, whole genome shotgun sequence.
ACCESSION   DADOOS010000113 DADOOS010000000
VERSION     DADOOS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.113"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..496)
                     /locus_tag="J8C37_002204"
     CDS             complement(<1..496)
                     /locus_tag="J8C37_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305625.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEII"
     gene            complement(595..945)
                     /locus_tag="J8C37_002205"
     CDS             complement(595..945)
                     /locus_tag="J8C37_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAZ5305626.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1102..2631)
                     /gene="ahpF"
                     /locus_tag="J8C37_002206"
     CDS             complement(1102..2631)
                     /gene="ahpF"
                     /locus_tag="J8C37_002206"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAZ5305627.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2641..3204)
                     /gene="ahpC"
                     /locus_tag="J8C37_002207"
     CDS             complement(2641..3204)
                     /gene="ahpC"
                     /locus_tag="J8C37_002207"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAZ5305628.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3348..4010)
                     /locus_tag="J8C37_002208"
     CDS             complement(3348..4010)
                     /locus_tag="J8C37_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305629.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4194..5840
                     /locus_tag="J8C37_002209"
     CDS             4194..5840
                     /locus_tag="J8C37_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAZ5305630.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5831..6913
                     /gene="uxuA"
                     /locus_tag="J8C37_002210"
     CDS             5831..6913
                     /gene="uxuA"
                     /locus_tag="J8C37_002210"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAZ5305631.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7021..8574)
                     /locus_tag="J8C37_002211"
     CDS             complement(7021..8574)
                     /locus_tag="J8C37_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5305632.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
BASE COUNT         2749 a         1575 c         1562 g         2703 t
ORIGIN      
        1 tgataatctc ctggatgccc atgaaaatca aaataaagcc gaatatttgg aatagaagca
       61 tcaaactttt gaatggattg aacaataaga cgaatccgcc aatcaacaac agaatactac
      121 tataaacact ccaactttta gcttttgtat tcatgctgaa gaacaactgg aacaagctgt
      181 tgatgataac agctaatcca agaagaatcg gcaggataga aacgattgca ggtgcaaaag
      241 catagacgat cagagctaag atcaggaaaa aaagaccact gaacagaccg atactccaag
      301 aacctgtttt ccttttaaaa cggtaagcct caaaaagatt caacaaccca agcaaaacaa
      361 gataggcggt gatcagataa ccgataaaat gaaaaacagc agaagggttt agcacaatcg
      421 caatcccaga aataatataa acagcagctc ttaataaggc gtatttttga atattttgtt
      481 taatcgtctc aaacatataa gaaaacctcc tttgacttat tttaacgtga actagtcatt
      541 ttaccaaatt aaatgttaca aaatgagcga aggagaagaa tatctctgga aaaatcaact
      601 tctgaggatc ttttccattt ttttgccttt tgctagctca tcgatcattt tatctaaata
      661 acgaatttct ttcatcaagt tatcctcgat gtcttctact cgtacaccac aaacaacacc
      721 tgtaatcaag gaacgtgaag gattggggac aggggcttcc gcaaaaaatt gttcgaaagt
      781 cacttgtttt tgtagttgat attctagttc tgtttgggaa taacccgtca gccaacaaat
      841 gatttcatct acttcctctt ttatacgatc ttttctctcc gcttttgcta catacatagg
      901 ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc gccataatca gtcacctttc
      961 tttttttcaa attatttact acttatagta tataagaaaa agagaagaaa aagcgattat
     1021 tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc atcctttttt ccgctctctg
     1081 actttttaca atcttcatag ctcaagtgcg catcagataa tcgaatgctc ctaaagctgc
     1141 agtagctcct gaacccattg aaacaatgat ttgtttgtat ggactatctg tacaatcacc
     1201 agctgcgaaa acgccgggga tattcgttgc gccatttttc tcggtcacta tttcatttct
     1261 tggattcatt tcgatcgttc catctagcca atccgtatta gggagtaagc cgactagaat
     1321 gaatactcct gaaacagcta gtgtatgcat ctccatcgta cggcgatcga tataggagag
     1381 agattctacc tgattcgtac cgctgatttc tttcgtcgct gcatgtttga taacagtgac
     1441 attcgcaaga gagactagtt tatcctgtaa gatttgatct gcttttaatt ctggtaagaa
     1501 ttctagaaca tatacatgtt ttgctaaccc agccaaatcg attgctgctt caacaccaga
     1561 attaccacca ccgataacag caatctcttt tcctttaaat aaagggccgt cacagtgtgg
     1621 gcaataagca attcctttat ttttaaactc tttttcgcct ggtacattga tagaacgcca
     1681 gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt gatataccgt tagctaagcc
     1741 aatattaata aaatgttgct ctttatttaa ggaaacggcc tgttgattca tcataatgtc
     1801 gatagggtaa ctacgcacat gactttctac ttgctgcatc aattttggac cttctgtgta
     1861 agaggtacca atcatatttt caatccctag tgtttcaacg acttgtccgc cgaaagaatc
     1921 agcgacaagt cctgtacgga ttcctttacg tgctgcatag atagctgcac tactagctgc
     1981 tggtccaccg ccaattacta atacatcata aggttctttt tcaaacaact cttcttcaat
     2041 caaagggcca gtgatttttt caacaatttg ttcgatcgtc atgcgtccgc tactaaattc
     2101 ttctccattt aaaaacacag ttggcactgc catgattttt ttatcatcga tttctttttg
     2161 gaacatccca ccttcaatca tcgtatgagt aatatttgga tttaatacag ctaatatatt
     2221 caatgcctgt acaacgtcgg ggcaattatg acaagtcaag ctaacataac tgtcaaaatg
     2281 caatgtttgt gttatttttt tgattttaag actcaaagat tcactgattt tcggtgcccg
     2341 tccactaatt tgtaacatag ccagtaaaaa agaggaaaat tcatgaccga gggggatacc
     2401 agcaaaaacg attccacttt ctttgccaac tggattgata gaaaaactag gtgtgcgcat
     2461 cgaatctctt ttttcccaag agattttagg agacatttct gctatttcct tcgtaaattc
     2521 agctagtttt tgactatttt cactattatc caaactaaca gaaaaaacaa taggagattc
     2581 aatcagtgct aaatattgtt cgagattttg tttagtttct ttgtctaaca tatccaccac
     2641 ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt tcgccacttt ctttccattt
     2701 tgccggacaa acttcacctg ggtgggtacg tacatattga gcagcgcgaa tcttatcaat
     2761 cagtgcacta gcgtcacgac cgattccatc tgcattgatt tccatagctt gaacgattcc
     2821 gtctggatca ataataaatg ttcctcgctg agctaatcct tgctcttcat ccaaaacatc
     2881 aaaaatacga gaaatctgat gagacggatc accgatcatc acgtattcga ttttaccgat
     2941 agcatctgat gtatcatgcc atgctttgtg agtaaagtgg gtatcggtag agcaagaaaa
     3001 gacttcaaca cctaaagatt tcaatgtact gtattgttct tgtaagtctt ctaattcagt
     3061 aggacaaaca aaagtaaaat cagctggata gaagcaaaga atactccagt tccccataag
     3121 atctttttca ctgacttcta aaaattctcc ttgatgataa gcatgtgcat taaattcttc
     3181 gatttttttc ccaatcaatg acatagtaaa acctccaata ataattattt atttataatt
     3241 attgtaattt agttggagtt gcaaagtcaa tggatatttt gttatttaat aaagattact
     3301 caaaaacgaa ggttaattat aaaaatagaa gaggtcgcaa gaaaatgcta ctgctggaaa
     3361 tactcgggat aacgttgagt aacagtttcc tgttctttca gcatcatgtg taagtgcaat
     3421 cccgtttgaa atcgagcttc ttctggtttt ttatcaacta aagccttgag taaatcataa
     3481 tgttgttcca tgatcgtttc ccatggaagc ccctgaatgg tcactcgtaa ccagcggaaa
     3541 cgttctaaat gggtattatg gtcatctaac cagttccaaa cttcttttct tcctgcaatt
     3601 tcgaaacaag cttcatgaaa taaattatct gcacggaaaa aagcgcgttc gtcatttatt
     3661 ttgactgctt tttcttgttc ttctaaaatc gtttctagca ctattcgtgt ttgtgtgtcc
     3721 aatttggaac aacattcaat catgatttgc tcttccaact gttctcggac aaagcgagca
     3781 ttttctgcag aaacaagatt aattttggaa acatacgtac cactttgagg tatcgtatac
     3841 acgagttctt gctgacgtag ttgaatcaat gcttcgcgaa tgggagtccg gccgatagca
     3901 agatgttcct ccagtgcctt ttcagatatt ttccttccag gttctaagtc agcatagata
     3961 atttgtttac gaatcgcttg ataagcttgt gattgcaagc tgcttttcat ctttattcct
     4021 ccaaaatcat ttctatgaac gtagtataac agaagaggtc tgaaaaacaa tctggtatgt
     4081 cacattaact agtaaatttg tataaatagt aaaattttgg ttgacatagc gatttttatt
     4141 gtataaaatt cacttgaata caaaaactaa tatgttagtt taggagggga aaaatgattg
     4201 atttaaacaa ctggaggaac tttatccctg aattatctca aaaagaagta gaaatacctc
     4261 attttgaccg tgcatcatta agagaaaatg gacaaaaaaa tcctgtctgg atccatttcg
     4321 gtggcggaaa tctttatcga ggatttcatg cagaaatcgc tcaacgttta gcggatctag
     4381 gcgaattgtc cactggtgtc attgtttgtg aaacattcga tgaagaaata attgaaaggg
     4441 cttaccaaga ttttgatgat ttgattcttg aagtggtcat gccaagcagt ggagagttcg
     4501 tcaaacgtat attagctgcg acagctgaca gtctgtattg tcatccagat tgttctgaac
     4561 actttgataa gatgattcga cactttgaaa atcctagttt acaattagtt acttttgcta
     4621 ttactgaaaa aggttatcga ttgaaagata caaataataa atatatagga ccgattgcag
     4681 tcgatataaa aaatggtcca aagaagccat cacatacaat gagtattctt gctgcgctac
     4741 tttataaacg ttttttatct ggagcttggc cgattgcact tgtctctaca gataattttt
     4801 cacaaaacgg tagtcaatta cgtacttctt tgctaacgat cgcaaaagaa tggcaaaagg
     4861 aaggttatgt acaaaataca tttatccaat acttatcgaa tgaagaaatt gttgcgtttc
     4921 catggacgat gattgatcgt atcaccccga atccttcctt gtcagttagc aaaatgttgg
     4981 caaaagaggg cttttcaaat atgtcgattt tccaaaccgc aaaaggaaat aatattgctc
     5041 catttgtcaa tacagaggac atccattatt taatcatgga agatcatttt cccaatggac
     5101 gtccgaaatt cgaaaaagct ggggtattct tgactgatcg tgcaacggta gataaagcag
     5161 atacgatgaa ggtgacgact tgccttaatc ctttgcatac gtctttagct attatgggtt
     5221 gtttactggg gtataggagc atagctgagg agatgaaaga tccagattta tcgctgctag
     5281 tttctgaaat cgggtacaat gaaggcctcc cagttgtgga gtctccaggt atcattgatc
     5341 caaagatctt tctggaagaa gtaattactg tgcgcttttc taatccaaat attcctgata
     5401 tgcctcagcg aatcgccacg gatacctctc aaaaggtggc gattcgttat ggtgagacga
     5461 ttaaaaaata tatggaaata gaagttaaag acccgcagac acttgtattc ataccattga
     5521 ctctcgctgc ttggcttcga taccttattg gcattgatga tcaaggaaaa ccattcatcc
     5581 cgagtccaga cccactattg acagactttc agcataagct ttccgatatt caactgggta
     5641 cgcaagatac atcacatatt catgagaaaa tggctcctat tttgtcaaat aaagaaattt
     5701 ttggtgtaga tctttaccaa gcagagcttg gcagaaaaat agaagaaatg ttcatcaaga
     5761 tggtagcagg accgaatgcc gtacgaaaaa cattacagga ttacttacaa gaacatggag
     5821 gaaatttatc atggaaatga cttttagatg gtacggatca caggaagaaa agattacctt
     5881 agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga tggatatacc
     5941 agtaggagag gtatggccca gggagaggat caaagaacta aaagaagaaa ttgagtctcg
     6001 tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta aaattggatt
     6061 accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact tggcagaata
     6121 tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga aatctgacct
     6181 tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg atattccaga
     6241 tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg aattgccggg
     6301 atgggaacct gagcgcttag ctcatatcaa atccttgctt gaagcctatg catcagtaga
     6361 cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc caacatgtga
     6421 agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga tttttggatt
     6481 acctcgaatc gtaaagaatc gagaagattt agattggttg tgccgcatag tagacagccc
     6541 ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa atgatgtata
     6601 tgggatttta gcagaatttt gtcaaagaga tcgaatccca tttgcacatg ttcgaaatat
     6661 taaattttta gctaatcgag atttctatga ggcaccgcat aaatccgaat acggttcatt
     6721 agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacgaat acatccgtcc
     6781 tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct tatatgatcg
     6841 agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag gaaacaaaag
     6901 taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg atgttcaaat
     6961 tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat cttatttggt
     7021 ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt cttttaattc
     7081 aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg tgatgattcc
     7141 ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaaagggc agtcgctgat
     7201 aacgatgttg cagttctcca taaaatcatt ttgtattttg atcgattcat ttgaaaaaat
     7261 ttgttctatc ttaacgatca gatctttact gatataaaaa tcacgcgtat agttcacacc
     7321 aatcacaatt cgcggctgtt ttttgtagcg atcatacaaa tgtactaggt cttcaaccat
     7381 ccattgcaca ttttcctttt ctaaaataac tggtaacaag tgttgatttt gttctctgaa
     7441 atccatataa aattgttcaa tggcggtttg gacttctttc aacacgctat gttcttgata
     7501 aagcatgaaa gtaggaaggg gcgtatttgg ttgattgaag ttaagaaatc cattataaag
     7561 tttatttcgt gcgaatgcca atgagtaaag aatcttttct ttttcaggta taaacaaatc
     7621 aaatacagta caaaaggact ccaccatttc tcttgaataa agaacatgag gaacgtttgc
     7681 tttttttaaa agtttgtatt gctccaagct tgtttcttcc gattcgattt tcgtgataga
     7741 aagtcgagct aaaatattca ctaagatgcg ttgttcgcga caaattggca gattaggtcg
     7801 caaaatatct acagagggat caataaaaaa gtcaatatat tcatactctg tgaattcttt
     7861 ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta atagaaaata
     7921 taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa aaaaagtatt
     7981 catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag tacttgaaaa
     8041 gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc cagaaaaaga
     8101 ctttctgcca ttattagaaa aggagataga caaaccgtac aatgctaaaa atgcattaat
     8161 ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac ttaaactgat
     8221 aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga ctgattcgtt
     8281 gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt caaaattcaa
     8341 ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac gctcaaagtc
     8401 ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga aatgatctgc
     8461 taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa tatctaattt
     8521 tgtattgctt tttttcttca agagaaacgt tttaatcgct tcttgagttt gcatgaaaga
     8581 ggctccttt
//
LOCUS       DADOOS010000114         8494 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.114, whole genome shotgun sequence.
ACCESSION   DADOOS010000114 DADOOS010000000
VERSION     DADOOS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.114"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..173)
                     /locus_tag="J8C37_002212"
     CDS             complement(<1..173)
                     /locus_tag="J8C37_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305633.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="J8C37_002213"
     CDS             complement(593..1714)
                     /locus_tag="J8C37_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAZ5305634.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="J8C37_002214"
     CDS             2042..3451
                     /locus_tag="J8C37_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAZ5305635.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="J8C37_002215"
     CDS             3448..4176
                     /locus_tag="J8C37_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ5305636.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="J8C37_002216"
     CDS             complement(4304..5215)
                     /locus_tag="J8C37_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5305637.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="J8C37_002217"
     CDS             complement(5232..6239)
                     /locus_tag="J8C37_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAZ5305638.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="J8C37_002218"
     CDS             complement(6236..8365)
                     /locus_tag="J8C37_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ5305639.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="J8C37_002219"
     CDS             complement(8370..8477)
                     /locus_tag="J8C37_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305640.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DADOOS010000115         8410 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.115, whole genome shotgun sequence.
ACCESSION   DADOOS010000115 DADOOS010000000
VERSION     DADOOS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.115"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(777..2408)
                     /locus_tag="J8C37_002220"
     CDS             complement(777..2408)
                     /locus_tag="J8C37_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAZ5305641.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="J8C37_002221"
     CDS             complement(2422..4095)
                     /locus_tag="J8C37_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5305642.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="J8C37_002222"
     CDS             complement(4108..4425)
                     /locus_tag="J8C37_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAZ5305643.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="J8C37_002223"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="J8C37_002223"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAZ5305644.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="J8C37_002224"
     CDS             6087..6872
                     /locus_tag="J8C37_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAZ5305645.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="J8C37_002225"
     CDS             6875..7567
                     /locus_tag="J8C37_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ5305646.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="J8C37_002226"
     CDS             complement(7841..8212)
                     /locus_tag="J8C37_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAZ5305647.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2587 a         1833 c         1437 g         2553 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta
//
LOCUS       DADOOS010000116         8159 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.116, whole genome shotgun sequence.
ACCESSION   DADOOS010000116 DADOOS010000000
VERSION     DADOOS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.116"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            115..816
                     /locus_tag="J8C37_002227"
     CDS             115..816
                     /locus_tag="J8C37_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAZ5305648.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1425..2441)
                     /gene="tsaD"
                     /locus_tag="J8C37_002228"
     CDS             complement(1425..2441)
                     /gene="tsaD"
                     /locus_tag="J8C37_002228"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAZ5305649.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2438..2902)
                     /gene="rimI"
                     /locus_tag="J8C37_002229"
     CDS             complement(2438..2902)
                     /gene="rimI"
                     /locus_tag="J8C37_002229"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAZ5305650.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2909..3451)
                     /gene="rimI"
                     /locus_tag="J8C37_002230"
     CDS             complement(2909..3451)
                     /gene="rimI"
                     /locus_tag="J8C37_002230"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAZ5305651.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3435..4259)
                     /gene="tsaB"
                     /locus_tag="J8C37_002231"
     CDS             complement(3435..4259)
                     /gene="tsaB"
                     /locus_tag="J8C37_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAZ5305652.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMVTLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4348..5328)
                     /locus_tag="J8C37_002232"
     CDS             complement(4348..5328)
                     /locus_tag="J8C37_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ5305653.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5352..6836)
                     /gene="galT"
                     /locus_tag="J8C37_002233"
     CDS             complement(5352..6836)
                     /gene="galT"
                     /locus_tag="J8C37_002233"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAZ5305654.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6848..7837)
                     /gene="galE"
                     /locus_tag="J8C37_002234"
     CDS             complement(6848..7837)
                     /gene="galE"
                     /locus_tag="J8C37_002234"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAZ5305655.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8085..>8159
                     /locus_tag="J8C37_002235"
     CDS             8085..>8159
                     /locus_tag="J8C37_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAZ5305656.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVAS"
BASE COUNT         2308 a         1731 c         1442 g         2678 t
ORIGIN      
        1 aattatattt gtaaagtatt aaaacttgtt tctaatgttg gtatgttaaa actttacaaa
       61 ttctttaaaa aaagtgacaa taaatatagt gaacaataag gagaggactt tttaatgcgt
      121 gttaataaaa tcgattttca cttttcaaaa ctcagatatc aacctgattt tgaacatttc
      181 tcggatgaag agttccaaca gatcaaggaa catactgttc ttcgttctta taaaaaagga
      241 cagatccttt tcgacgatgg tgatcccaga caacggttgt atatcttgac caaaggactc
      301 gttcgtttag aacgttatga tgaaagtgca acttattact actatgacta tgtgaataca
      361 aataaccttt ttcctatgtc aggaatgttt ggagaagaga cctacactta tacagctcaa
      421 gcgatgactg atattgaaac attctacctt cctgtttcct actatgaaaa attagcacga
      481 aaaaatcccg aacagttgat ctatatgata caaaaaattt ctaaagtagt tgaaatacat
      541 gaattgcgta tccaaatcgg attgacctct agcgcctttg accgggtaaa acaaaattta
      601 ttcattttga aagaagaatt gggcgtacca acaaaagatg gtcatatcac cattccttat
      661 ccgatcacct taaaagaatt agcagtgaat agcggcacga cacgtgaaac tgctggacaa
      721 gtaatcaaac aactacgtga ttccgggctg ttagattatg acaaaaagcg ttttactttc
      781 tgtaagacag ctactactcc agctatgaga gaataacgcg aacaaaaata agcagtgacc
      841 ctgataaata tcagggtcac tgcttatttt cctccctaaa acttctgaaa gatccggcag
      901 ttttagatca ctggacaaga ctcaaagatt ttctatgtgt gtttcagtta tgtaatattc
      961 actgtttgca aaaaatactg ttagattaac tagatgaagc gtcttgtcct catccgatct
     1021 agattattcg atattcctcg acaataaacg attactcagt gagggcaatc agaggaacgg
     1081 ataatgataa ataagctgta aaaaatagca aaataacaat aatgattaaa agtatgtaaa
     1141 aaatagggct ttgacaaaaa cgtgtcacag ccctatttcg attaaacggt gtgtacctgt
     1201 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
     1261 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
     1321 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
     1381 gctgttctta ttacttgatg cagggcagct ttttcacaac ctctttattc agataaaacc
     1441 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
     1501 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
     1561 tctacctcag ggagtttagc tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
     1621 gccgctacac ctccagctat aatcagttgc ttgactggat aattttgaca agctcgaagt
     1681 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
     1741 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
     1801 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgcggaaa atggtaatta
     1861 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
     1921 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
     1981 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
     2041 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
     2101 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
     2161 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
     2221 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
     2281 tgatggcggc tagctacttc aggtactacg ccaccaaaac gtttatgact gtttatttga
     2341 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
     2401 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
     2461 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
     2521 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
     2581 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
     2641 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
     2701 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca gctaaaagca
     2761 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
     2821 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
     2881 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
     2941 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaacccca
     3001 atttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
     3061 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
     3121 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
     3181 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
     3241 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
     3301 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
     3361 aacgacgatc gatttgttcg actgtttttt ctgtataaat cattttatct tgaaaaaagc
     3421 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
     3481 aaccattttt cttctgcttc tactcgtttt aaatagcgag gaagaaaagc ttgtttctct
     3541 gcaactggtt caactaaagc acctaattga gccacagtta caccacttgg aatatcccat
     3601 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcaga gaagaatttg
     3661 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
     3721 tcagaagcag aaatatgtac atcttcaagt acagtttcta atctattttc tttccagcgg
     3781 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aatattatcc
     3841 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
     3901 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
     3961 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
     4021 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
     4081 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
     4141 attcctaatg taaccatata tttgatcctt tctgctatga cattctcgcc ttccattgta
     4201 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca ataatacatg
     4261 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
     4321 tccattttcc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
     4381 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
     4441 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagcg agggcgttac
     4501 atatttcgct acactgacat cattgaagcc catcacagaa atatcttctg gtaccactaa
     4561 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
     4621 cgcatcagga acatgcggat tcgtttttaa atattgcttc actgcttgat atcctgactc
     4681 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
     4741 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
     4801 tccagcaagc atagcgatat gttgcgcttt atgggagaca aagtgttcaa tgactttttg
     4861 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
     4921 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
     4981 tcctagtgcg atcgttccat ctgccggaag atcactaatg gaatcccacg tttctttcaa
     5041 taaagtaacg tttgcttctt ctgccttttt ttcgattcct aggcgaatag ataaataata
     5101 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
     5161 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
     5221 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
     5281 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
     5341 cttttttacg tttataaaat cgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
     5401 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
     5461 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
     5521 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
     5581 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
     5641 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
     5701 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
     5761 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
     5821 actgtttcat ccgaatatgc tttccatttt tccaatactg agttagctgc ttcaataagc
     5881 aagtttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
     5941 atgacttgcg gataatcttc tagtttgata agtttttcta tcggtgcttt tgccatagga
     6001 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaattggt
     6061 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
     6121 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
     6181 tcattgtaat aagcataagg ggaatattgg aatccccagc tttcaccgtc tagattcatt
     6241 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
     6301 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
     6361 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
     6421 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
     6481 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
     6541 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
     6601 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
     6661 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
     6721 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
     6781 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
     6841 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
     6901 taatcgcttt gatatctgtg tattcaggtt tccagcccag aatctctctc gctttttcac
     6961 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
     7021 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
     7081 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
     7141 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
     7201 cataatcgtc tccataaaca gccaaggctt ttctttgacc aagagctact tgtaaaatga
     7261 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
     7321 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
     7381 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
     7441 tatcttctgt gattggagac tctttcggct ctccataagt tgctgcagta gaagagaata
     7501 cgattttatt cacgttgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
     7561 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
     7621 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
     7681 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
     7741 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
     7801 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
     7861 aaattgtgtt tgttgattct atataattta aaaaacgaag tttactttta tggtgaaaac
     7921 aaacaaagta aaatttcgta aattcatatg gtaaatttat aataaaacat ttagtaaaca
     7981 ttttcaaggg ttttttgcaa aggtttacaa acaaacgtaa aactagcctt tttttctctt
     8041 ttgttccata atagatataa caaataataa atgaggtgga ttcaatgaaa aaatttgtat
     8101 ctggaatatt agtaggaagt ctggcaacag ccgctgccgt tgctggtttg gttgcttct
//
LOCUS       DADOOS010000117         8144 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.117, whole genome shotgun sequence.
ACCESSION   DADOOS010000117 DADOOS010000000
VERSION     DADOOS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.117"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            115..978
                     /locus_tag="J8C37_002236"
     CDS             115..978
                     /locus_tag="J8C37_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ5305657.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1107..2729)
                     /locus_tag="J8C37_002237"
     CDS             complement(1107..2729)
                     /locus_tag="J8C37_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5305658.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2978..3499
                     /locus_tag="J8C37_002238"
     CDS             2978..3499
                     /locus_tag="J8C37_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305659.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3685..5415)
                     /gene="spxB"
                     /locus_tag="J8C37_002239"
     CDS             complement(3685..5415)
                     /gene="spxB"
                     /locus_tag="J8C37_002239"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAZ5305660.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5580..6398
                     /locus_tag="J8C37_002240"
     CDS             5580..6398
                     /locus_tag="J8C37_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAZ5305661.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6501..6698)
                     /locus_tag="J8C37_002241"
     CDS             complement(6501..6698)
                     /locus_tag="J8C37_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305662.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6867..7445
                     /locus_tag="J8C37_002242"
     CDS             6867..7445
                     /locus_tag="J8C37_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAZ5305663.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2468 a         1436 c         1607 g         2633 t
ORIGIN      
        1 acctcttatt ttgattagaa cttgcgaaaa taaaccggtt aaagtgtgtc tagcgcaaga
       61 gatttgctat aatagacgat aagggtaaaa tgtaacgaga aggggttgaa aaatgtgtat
      121 caaattgtaa cagattcttg ttgtgatctg ccataccatg tattagcaaa agaaaaagtt
      181 gattttttat caatgaatct ccaattgaat gatcaagagt tagtagatga cctcggaaag
      241 acattcaatt atgaccgcta catgaatcag ctgaaaaatg gtgcgatgcc tacgacatca
      301 caaatcaatg ttggccgata tttagaattt ttccgttctt atgcggaaaa acagattcct
      361 gttttatatg tggctttttc cagtggatta agcggctctt acagtagtgc gcttcaagcg
      421 gttgaattgc tgaaggaaga atatgaagac cctgagatct atgtttttga tacaaaagct
      481 gcaagtattg gggaagggct tttagtattg gaagctgtaa gattgaaaaa agaaggtaaa
      541 agcatcgaag aaaccctaga gtggctggaa agtaattatt tgaaagtcca ttcatgggta
      601 acagtagacg atctgaaata tctagaacga ggcggtagga tttccaaagc aagtgctact
      661 cttggcagtc tattgagtgt aaagcctatt attacggtgg ataaagaagg aaggctgacg
      721 aatgttgaca aagttcgagg aagaaacaag tcgatccaga caatcgtcaa tgagacggta
      781 aaaacaatcg ttgcaccact tgatcaaatc gtttatgtgg cttacgctgg ggatctagag
      841 agcgcggaga aggcaaaacg gcttttggaa gagcagataa agatgaaaga tgtgaagctt
      901 tatcccttag gaccaacgat cgccagtcac acaggctatg gctgtatcgc gattttttcg
      961 atgggaatca gtcgataaaa gcagtaactc attctgtaaa aaagcccaga aattgattgt
     1021 aacaaaaaca gcgactctta aaagatagat gagcatccta tctcttagag gtcgctgttt
     1081 ttttattttt taagctaaat aggccattat gcccataact cgttcaattg tttttgaata
     1141 tctgcatttt ccaaaaattc atcatatgtc gtatcagctc gatcgatcac acctttatcc
     1201 gaaacggcaa tgatgcggtt tgccaatgtt tggataaatt gatggtcatg tgacgcaaat
     1261 aagattgagc cagtaaatgc catcaagccg tcattcaatg ctgtgattga ttctaagtct
     1321 aaatggttcg ttggatcatc taatacaagt acgttagctt tcgaaagcat catttttgaa
     1381 agcatgcaac ggactttctc tccaccagat aaaacattca ctggtttcaa tacttcttca
     1441 ccagaaaaca acattcttcc taagaagctt cttaagaagg tgttatcatc ttcttcttta
     1501 cttgcatatt gacgcaacca gtctaaaatc gttagatcag tatcgaattc tttcgttgta
     1561 tcctttggta aataagaatt acttgttgtc acaccccaac gtactgttcc agtatctggc
     1621 gtaatttcac ccatgataac tttgaatagc gttgttgttg ttatatcatt ttctgcgacg
     1681 aaagcgacct tgtcttcttt gctcaaatta aaagatatat catctaaaat tttctttcca
     1741 tcaatcgtta cacttacatt ttctacctgc aataagtcgt ttccaatctc acgttcaggt
     1801 ttgaatccaa cgaatggata acgtctagaa gaaggttgga tatcgtctaa agtaattttt
     1861 tcaagcattt ttttacgtga agttgcttgt tttgattttg atgcattcgc actaaagcgg
     1921 gcaataaatt cttggagttc tttgatttgt tcttcttttt tggcatttgc gtttgcttga
     1981 agtttagccg ctagttgact agattccaac caaaaatcat aatttcctac ataaagtttg
     2041 attttaccaa aatctagatc cgccatatgt gtacatactt tgttcaaaaa gtgacggtca
     2101 tgggatacaa cgatcacagt attttcaaaa ttgatcaaaa attcttccag ccaattgatc
     2161 gattggatat ccagaccgtt tgttggttcg tctagcaaaa gaacatcagg tttaccaaat
     2221 agtgtttgtg ctaataacac ttttaccttt tgtccaccag ttaattcact catttgctga
     2281 tggtgcaatt cttcagggat gttcagacct tgcagtaaaa cagctgcttc tggttcagct
     2341 tcccatccat taagctcagc aaattctcct tctaattcag ctgctcgaat accatcttcg
     2401 tcagtgaaat cctctttcat gtaaatcgca tctttttctt tcagcacttc atacagacgc
     2461 ttgtgtccca tgatgactgt ttcaagtaca gtatagtctt catagtcgta atggttctgt
     2521 ttcaatacgg ccatccgttc atctggtccc atagtaacgg aacctgtgga aggctgcaga
     2581 tcccctgata acactttaag gaacgttgat ttacctgcac catttgcacc aatcaagcca
     2641 tagcagtttc cgggggtaaa tttaatattc acatcatcaa atagtttgcg gtctgaaaag
     2701 tgcaaactca catcatttac agtaatcaat ttttttcctc acttttatca ttattagttt
     2761 tgacaagtct tatagaatta tataggggaa aggtaaaaga ttcaaggcaa tcgaacattc
     2821 tttgtgtttc acgtgaaaca gaatgaaaaa aataaaaata aaagcggagc gaaaatagct
     2881 actataggat ttattagttt gcaacagcat aatttttgac gttatttata agagggagta
     2941 ctataaaact gtaaaagaaa tgaaagaagg attgaaaatg gatgaaaaaa gaaccatcga
     3001 ctggggctca ttgattttgg gaattttatt tgtacttgtc tcattgttat catttcaaga
     3061 ccctgtcgga aatttagttg caattgtagt agtctttgcg atttttgcct ttattaaagg
     3121 gatatttgaa ctgtttgtac ggaacagatt aaaagagcta acaggatata aaggaaagat
     3181 gcccttaata atcgggatca tcgatattct agttggcgtc tttttcttat tcaatattgg
     3241 tgcaggagtc gcagcattgc cttttgtttt tgcagtatgg tttattgctg attcagtttt
     3301 agcgttattt actgctgact tggcaagagg agtaagtgaa ggatattatt ggtttacgat
     3361 catcgtcaat attttaggaa ttttattagg aattttctta ttgtttaatc caatttcttc
     3421 tgcgttgaca ttaagctttt tagttggatt ctattttatg ttatttggta tcacacatat
     3481 cgtctatgca tttagataag taaaatcaaa caaataaaaa agccggcttc cgattacagt
     3541 tttactgttg taattggaag ccggcttttt tatcatggac aaatatgcac gaggtgattt
     3601 ttaggtgtcg agacaaattt atgcaaaaat gtgagaagct atatacacaa attttatctt
     3661 aatactcgaa gcatagcgca gttgtcacaa cctcttttca tataattcat tcaatactgg
     3721 caaatcatgg acttcatatt tttctttgaa tgcttggatt tcttcttcag aatattttct
     3781 tggatctaaa tgcagatctt ctactggcaa tgggcgcttg ttattgatct tcacatcgat
     3841 tactacagga cgatctgatt ttgcagctgc atcaaaggca aatcgaagtt gcgagtattc
     3901 tgtgactgta aagctatttg ccccgagcgc ttctccaact ttaccgaaat ctgcatcttc
     3961 taaaaataca ccaaatttct tttgttccgt atcttcttgt tctgcttcga tgaaaccaaa
     4021 agaatcattc gaaaagacaa cattaataat tggaagctga tatttgactt gtgtaatgat
     4081 atcttgcatg accatagcaa acccgccatc accacttaag gtaaatactt gtttttctgg
     4141 gtagcttagt tgtgcggcaa ttcctcctgg cacaccatta cccatagttg cgaaccatcc
     4201 agatgtcgta tgtttttgtt taccgttcat attcaataaa cgaatagaat ggatagtcgt
     4261 gttaccgaca tctgtgacga aaatcgcatc gtcttcagca atacgattga tttctttata
     4321 gacagcctct ggacgaatcg gttcttcctc tcgatcttca aaagatttca accaatttac
     4381 ccatttttct ttattttctt gatttgctag gtaccagcta tcagcagggc gagcatcccc
     4441 taactcgaca agtctttgca aagctgtgtt tgcatcaccg aggatcgaca aagatgtttc
     4501 atggcgtcgt ccaaattttg acgcatcgat atcgatttgg ataaattcag cagttggatt
     4561 aaaaaatgca cgtccaaatg ggaagtcgct accgacaaat aagatcagat cggcttctgc
     4621 caatgcttca tttccgggtt tagtagcgac acgtccagca aaaccaagaa agttttcata
     4681 atcatcagga ataattcctt ttgctagaac ggaagcgaca acaggaactg aaaaatgttc
     4741 tgagaattct ttgatttgcg gaaatccgtt tcgcgttcct tgaccaacat aaagaactgg
     4801 tttttttgct ttttcaagat aaggcaaagc agctagcaaa tctttttctt ctggtaatat
     4861 cacacttgtt ttgtgagtgt gagcggtgga gaaaggttgt tcgtcaattt cttcaaaacc
     4921 aagatcaaca gggatagtca cgatagccac gcctttatgc tcataggcgg ctttaatggc
     4981 ttcatctact acatgcggaa gactttgtgg tgtcatgact gtacgattgt atacgctaac
     5041 atctgcgaaa ataggatttt catttagttc ctggaaagag ttatagttca tggatgtaga
     5101 agcaacttgt cctaagaacg ctactagagg cacatggtcc atctgtgcat catacaaacc
     5161 attgatcaaa tgtgtcgcac caggtcctgc agagccgaag acagcgccga ctttccccgt
     5221 taattttgca tctgcagcgg ctgcgagtgc gccgacttct tcatgacgaa cttgaatgta
     5281 gttgatttcg gttttttctt gatatagcgc atccataatt gaattgaaag atcctccggg
     5341 aatgccgtaa atatggtcga taccccagct ttcaagaact tttaccattg cgattcctgc
     5401 attgattttt gacatggtgt ttcactcctt tagcaatgat gatacataaa ttttttgtat
     5461 ctagagtata gaacttttta tcgaaaatcc caactaattc gtcttttcac aaaaatgtag
     5521 tcatgttttt gggaatatgc tagagtagta catagataga ttcaagagag gggaatggca
     5581 tggttgaatg gctattgaga cttgaacctt ggcagcaagc acttgctggg acagctttta
     5641 cttattttat gacagctctt ggtgccgggt tggttttttt ctttaaagaa atcaaaaaag
     5701 aagtgctaaa cttgatgtta ggatttgctt ctggtgtcat gattgctgca agtttttggt
     5761 ctttgttaga tccggctatt acaaaagcag aagaaaatgg agatattgct tggctagttg
     5821 taagtattgg gtttggttta ggcggtgtgt ttttatatat ggcagataaa acactgcccc
     5881 atatgcattt tggaccacaa catgaagcag aaggattgcc tacccattta aaacggacga
     5941 tcttgcttgt tttttctatt acattgcata acattcctga aggtttagcc gtgggggtag
     6001 cattcggtgc agcagcaact gcggataatc cgacagccgc tatattagcc gctgtttcgg
     6061 tggcgctagg tattggtatc caaaacttcc cagaaggagc agctgtatca atcccgttgc
     6121 gacaagaagg tctgagtcgt aaaaaggcct ttgtttacgg acaagcttct ggaatcgttg
     6181 aaccaattgc tggagtgatt ggagcgctac tagtaacgaa agtggagctg ctgcttcctt
     6241 atgcacttgc ttttgccgca ggagcaatga tttatgtcgt ggtggaagaa ttgatccctg
     6301 aagcacagca gacattgtcc agcaaacgtc attttgcagt ttttggcgtc atgagtggat
     6361 ttatcattat gatgattttg gatgtggcat taggataaat aaaaaaaata tatatgatat
     6421 atcaaatagg gccgcgaact tatatcgcga ccctattttt ttagtaagtt atatcataat
     6481 gaagtgaatg gtatcataca ttaataatag ttttttctat gtgtatttcc tttgatccag
     6541 cgaacaagcc agatgataag tgcgattggg attagaactt tgaccgcaaa ccataaaagc
     6601 ccgcttagga cactgacaaa aagactgatg acgaagctag ctgcaattag cactaaaaga
     6661 accatgaaga tatttaagaa catatttttt tgacgcatac aaaaccctcc tcttgttcat
     6721 tctttactat agaaaaagta taacagggat tgtttaaaaa cacctccaac tgaagactga
     6781 tttttacttc atctacttaa atgatcaaac aattgctttt tctctggttt atcatctaaa
     6841 atagagcgga aaaggaggag aatatcatgc ctaaaaatcg tcttgaagca tttacagatg
     6901 ctgtaatcgc gattatcatg actattttag ttttagaact tcatgaacct aacggagaca
     6961 cgtgggaggc gctcgctgca ttaggttacc gattttttat ctatattgtc agttttatca
     7021 ttttagcgat ttattggaac aatcatcatc atttatttca agttgtacat aagataaatg
     7081 gacgggtctt atgggcgaac agtttctttg tttttgcgct ttcgcttttt ccgtttgcaa
     7141 cttcatggat cagtgagtac atcaatagcc tggctccaga aatgacttat ggttttgtta
     7201 ccttgaccgc taatattgcg tattatatct tggcaaaaga acttgtcaaa gcaaataaag
     7261 gccacgagaa agcattgaag gcgatgttga ataattacaa gaaatcgtat ctatcgattg
     7321 gtttgaatgt gttaggaatt gtcttagggt atttagtagc gccagtagcc gtgctgatcg
     7381 caaacatcgt gattctagct gcttggatca tcccaagtcg taccatcgaa tcacaatatc
     7441 ggtaaaatga tccgtgttta aaatttaaga gaaaaatata gaaaaaataa ataaacgatc
     7501 aaatatccga gtgatactag tattcaaaga gggatgcaga tggatcaaaa aagaattcat
     7561 gtaggactga ttactagttt tgtcacgatg cttatttatt tggatcaagg tcatttgact
     7621 tggacagtag ttggtgtcgt aggatttttg acattattca cagcaataaa aaaataaaca
     7681 tctttatgta atctgatgtt ctatctattt aagaaaagca tgtttacagc agtatgattc
     7741 tgttgtaaac gtgtttttat ttgtggaaaa tttaattttt agaaaagtgt atcgaaaaat
     7801 agatagttag tttttccaca gtggataact tcgtaagaga taaaaataaa aaattagtca
     7861 aagaagggcg tatagatttc ttttgatagg agatatacaa cagaaatgaa taaatattaa
     7921 cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa catatctcat
     7981 tttacgcttt tgttatttta tacttaaaaa ataacgacac agaaaatacc gtgtctcaga
     8041 tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg
     8101 atcaggcaga atatactggc agaatcaagg agaagggagc ctaa
//
LOCUS       DADOOS010000118         8068 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.118, whole genome shotgun sequence.
ACCESSION   DADOOS010000118 DADOOS010000000
VERSION     DADOOS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.118"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            157..657
                     /locus_tag="J8C37_002243"
     CDS             157..657
                     /locus_tag="J8C37_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305664.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(847..1191)
                     /locus_tag="J8C37_002244"
     CDS             complement(847..1191)
                     /locus_tag="J8C37_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAZ5305665.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1345..2265)
                     /locus_tag="J8C37_002245"
     CDS             complement(1345..2265)
                     /locus_tag="J8C37_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ5305666.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2452..2943
                     /locus_tag="J8C37_002246"
     CDS             2452..2943
                     /locus_tag="J8C37_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAZ5305667.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2967..5342)
                     /locus_tag="J8C37_002247"
     CDS             complement(2967..5342)
                     /locus_tag="J8C37_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAZ5305668.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5668..6294
                     /locus_tag="J8C37_002248"
     CDS             5668..6294
                     /locus_tag="J8C37_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAZ5305669.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6317..7021
                     /locus_tag="J8C37_002249"
     CDS             6317..7021
                     /locus_tag="J8C37_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ5305670.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7213..8046
                     /locus_tag="J8C37_002250"
     CDS             7213..8046
                     /locus_tag="J8C37_002250"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAZ5305671.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2481 a         1529 c         1455 g         2603 t
ORIGIN      
        1 gaacttcaaa taattttttt attgtaattc atatttgtat tgttcgatga ttcttaatac
       61 gatatgacac aataaataaa aataggcatg gctatttaca acaatagtgc tatagtagaa
      121 aacaaatgaa taaggttact taaagaggtg aatacgatga aactgatcaa actatgggaa
      181 gcagatttaa agaaagctta tcagctacaa actagctttg gaccaaatga gagtggattt
      241 atcaacaatg cttatggcta tacatttgct gaatttttag attatgtaaa aaaatgcgaa
      301 aaaacgtctt taggaatcga tcttcctgag agttacgttc ccgcaaccgt ctttatttta
      361 gaaagtgatg aacatgagta tgtaggaatt tttaatttgc gccataaact gaacgaaaca
      421 ttaagaaacg gcgctggcca tattggttac ggcatctccc ctaattatcg taaaaaagga
      481 tatgccacac aaggattagc attagtacta caagaggcaa aaaaaatcgg cattgatgag
      541 gcacttttgt ctgtaaataa aaaaaatctt ggcagtctta aagcacaaca aaaaaatggg
      601 gctgcgattt atcgtgaaga tgagaaagaa tactatacga aaatcaaact gatgtaaaaa
      661 agaaggattc caaaaggaaa gggatttgaa tgtcctttat caatagaaca catttattta
      721 attaacttat aatatagcag acaaaatttt ataaacaaag aaacctttta tctcttttct
      781 tctagaaagg ataaaaggct tttttgtttt tcctatttgc ttctcatagc tagtcagcca
      841 agatttctat cgtatcccca acgcaatccg tgcataacgg ctcatcttat ctgtcgtcca
      901 tgctggatac cagactagtt tgacttctgc tttcgttact tctggcactt cttttaatgc
      961 gtcatggata gattctgtta aaatatctgc taatggacag cccatagttg ttagagtcat
     1021 cttaatgact gtttctccat tttcaggatt gaattctata tcataaatca agcctaaatt
     1081 gacgatgtcg attcctaatt caggatcaat caccatttcc aaggcagcaa gaatacgttc
     1141 tttgattagt tctatttctt gtccagaccg ttcttggttt gcttcgctca tcgtacgaca
     1201 ctccttaaca cgatttttat ttatcatact ctttttttag ctgcttgtaa atgagaattt
     1261 agaagtaaga ttctcgttta aatttataaa aatgttctta ttagatgtaa tagattaaat
     1321 ttattctaaa ataaacctga ttcctcattt tttttgtgaa cgatttttta tttctggcaa
     1381 tacgccagtt gttcgataca ttttttgttc gttttcattt gcaaaccggc caaatactga
     1441 atgagttggt aaaatattga taaatgcatc gtcgtcgatt tcatgaacga tctgatcaag
     1501 atcatacatt tcgtagcggg tgatgaccat catgatcatg ttcccttgtt tttttgaata
     1561 gccaccgaca gacggcagta atgtcattcc acgtaccatc cgttttgcta gttcttctgt
     1621 tacctcttct ggcttgacgg taacgatcat tgctgttact ttttgatgac tcgtgtggat
     1681 ggcatcaatt acttgagtca ttgcataaat ggaaataatc gtatacaagg cactttccca
     1741 gttaaagaag aagcctgcga tgactacaat gataccattt agaatcagca tgtagtttcc
     1801 gactgttttt ccagttgttt gtgaaagtac aagagagatg atatccatcc caccagttgt
     1861 aaagcccatt tttaaagata atcctacgcc aagacctaat aaaacaccgc ctactagggc
     1921 attcatcaaa atattatcag tgatctgtcc gactggaagc agcatcgtca tcaccgatac
     1981 acatacaaca ttcacaaaac taaggatcgt tgctcgtttt cctactttga gaaaccctaa
     2041 tacaaacaca gggatattca aaagaaaaat aaatgttcct gtgtctatct tgatatgaaa
     2101 ccattcatat aaaagcgagg aaatgatttg agcgatccca ttcatccctg cagaaaagac
     2161 attggctgga atcaaaaaat aatttaaagc gaccgcagcg gtcaatccgg ttatcaaaat
     2221 aaccactatt ttctttaatc tttcattttt atgatacgca gtcataattt tactcacaag
     2281 cacctctccc ctactttggt attccaaatg taagaataac agtttatcta ttaaaataaa
     2341 atacttcgaa ctcaaatgag aagaatttta gcaaacaggt aagaatctgc cctaatccta
     2401 tgataagata gatgtatggt aaatatttat atgtatatca ggagggttcc tatggcaaaa
     2461 gatgctagct tcgacattgt atcagaagtc aatacagaag aagtaaaaaa tgcgattcag
     2521 caaacaacaa aagaattgtc caatcgcttt gacttcaagg attctacagt agaaatcaaa
     2581 ttagagaaca gcaatctagt tgtattgagc gatgatgatt tcaaaatcga acagatcaaa
     2641 gatgtattat ttggtaagct gatcaaaaga aatgtgccaa tcaaaaatat ccatttttcc
     2701 gaatcagaac acgctctagg tggcaaagct cgacaaactg ccgatttggt caacggaatc
     2761 gatcgtgaaa acgcgaagaa aatcactaca gctatcaaaa atgaaaaact gaaagtaaaa
     2821 gcacaaatcc aagaagatca gattagagtg acaggtaaga accgtgatga tctacaagct
     2881 gtgattgctt tattacgtaa acttgactta ccaatcgagt tgcaattcac taactatcgt
     2941 taatcaaaaa acgaggctga cgtctgtcat tcctcgtttt tttgatttac tgcttctttt
     3001 gataaccaaa aacgctgtat tccttcttcc aattccccaa cacttcgtac ttctcttgct
     3061 gtttgccaat gttgtgggaa taaacgtcga taagaaaaac tagaaaatcg ttgatctgcc
     3121 aatacaacga ttccttgatc cgccatgcca cgaatcactc gaccaccagc ttgcaagact
     3181 ttgttcattc caggaagctg ataagcgtaa gcaaatccaa ggtgttcctt ctcatcataa
     3241 tatgatttga tcagttcctg ctcatgattc atctgcggca aacctacacc tacgatcata
     3301 ctgccgatca atcggtcccc tctcaaatcg attccttctg aaaaaatccc gccaagtacg
     3361 caaaatccaa tcaatgtttc agcgggattt gtttgaaatt ttgttaaaaa tcgttcgcgt
     3421 tcttcctcat tcatgtccgt ttcttggatt agcacattgc tgtcgggata ttgctgtctg
     3481 aatgcttcca ccgcaagatc taaatattga taagaaggaa agaaaacgaa ataattcccg
     3541 acctttccat cagcaaaagc atgaatcgtc tctactaatt ttgccagact gtgttccctt
     3601 tttcgataag tcgtctctag gtaatttgcg atgaaaactt gctggtttcc ttctgggaaa
     3661 ggactcggca gtttgtaagc cagacttttt tgtcccccca gtgtttcctg ataataagag
     3721 agtggtgaaa aactagcaga aaaaagaata ctgctttttc ctttatccaa cgactgctca
     3781 agaaacaagc ttggatcaat acaaaactct tttactatca gatcgcgata cgttttttcg
     3841 actgttgttt cgtagtgatc atcataaaat tcactgacct tcaaaaaatg aagcaaatca
     3901 aaataataag gaagcaattg ttcttgtgat gggtgctcag gaaattctgc cagccattct
     3961 ttaatttttt cgcttaattg ataacctgcc tttactaaag attcagccgg tgctttttga
     4021 tgatgataat cccaatgatc ttcttttgct atttctcgga tcgaatcaaa ttcttttaat
     4081 agtttgttga aacggcgatg aagttttttg aattcctttg gtatagcttc cttacttcgt
     4141 ttcgtttttt cataagatag ttctgccgaa tacatctctc gggaacgatt caccaaatta
     4201 tgtgcttcgt caatcaaaaa cagatagtcc tcgttttttt catcttcaaa aaaacgtctt
     4261 aaataaacag ttggatcaaa taaataattg taatccccaa tgattacgtc acaccagata
     4321 ctgacatcta atgatagttc gaaaggacaa agtgtatgct ttcttgcata agtttcgatg
     4381 acttcacgag tgatctgatt ctcatgattc aaaagatccc ataaaccttc attaatccga
     4441 ttgtaataac cattggcata tgggcactga tctggattac aggtcgtctc atctaaaaag
     4501 cagatcttgt ctttcgccgt caaagtgaca ctttttactt cagctcctgt atcttttaat
     4561 gctgtcatag catcttcagc tacttgtcta gttattgttt tagccgttag ataaaagatc
     4621 ttttctccct cttcttcccc tacagctttc aaagcgggga ataacgtaga gattgttttt
     4681 cctgttccgg taggtgcttc tacaaacaat ttctgtttcg tacggatcgt tttataagca
     4741 gctaccgcta gttcacgctg acctttccta tactcatcat gggggaattt caaagccatc
     4801 aaagaggcat tgcgtacagt gcgccagttt tcttgaaaaa caagccattc tttataatct
     4861 tcagtcagct ttttaaaaaa agtttctaac tgctctcttg tctgatgttc aacctttctc
     4921 gtgatcactt cttctgccgt ctggaagtaa gtcaattgca gatttatttc ttgtaaatca
     4981 tgttcttgac tatagatata ggcatacacc cttgcttgat gaaagaaaag ttccacttgt
     5041 tctggttcta aatcttcaaa tcgtggttcg gaggttttga tttcatctat ataataaata
     5101 ccgtctcttt tgaaaatacc gtctgcacgg ccttcaacga tcaggtaata ttcatctaat
     5161 tgaactgttg tttttaaaaa gacttctttt tcatagtttt ctcccgcatc tttttggagc
     5221 ttgcggtgga ttctcgctcc ttctaatgca gtatggttac tttttttacg attgtcgata
     5281 cttccacgtc gcaaaataaa ttctactaag tgccgcacag atatttttga ctgtttgatc
     5341 atttccacca cctctttctc tctaagatta cctgaaaaaa cctagtttag caaaaagagc
     5401 tctctttcca aacataaaaa aacaccctgc tgattaggca gggatatagg atatagaatt
     5461 tggatttttg gatgaatcta atgtaacaag gacatattaa aatgctatga aaacttactt
     5521 tccttctctt tagaatatcc aaatactttt catatagtct gataattcag ataagtaaag
     5581 agaaataagt acctgttttt gtttcctagc aatgaatgcg ttatactaat ggatgaaaga
     5641 agttttggct atcaaggagg aatgcacatg catatcgaaa gattaaaaac aggaatgatt
     5701 gaagaaaact gttatttggt ctacaacgaa gaagcacttt tgatcattga ccctggagaa
     5761 gatgcggaac gaatcaaaca agaaatcaaa aaaacaaacc aacagccggt agcgattttg
     5821 cttacccata ctcactacga tcatatcggt gcagtggaac ctttacgcgc gtattatcaa
     5881 atcccagttt atgtcagccc actagagcaa aaatggctag gtgatccgat tttgaatctt
     5941 tctggactcg gtagacatga tgatattgct gatgttattg tctcgcctgc agaatatgaa
     6001 tttgaaatga aaacttatac tttaggaaac atgtcttttc gcgtcgtccc taccccaggg
     6061 cattctatag gaagcgtcag ctttattttt gatgattttg tcgtttctgg cgacgccctg
     6121 tttagaggaa gtatcggtag aaccgatctg tatacaggga atttagaaca attacttcat
     6181 agtattcgga cccagctgtt tgtattacct gatgaatttg cagtctatcc aggacatggc
     6241 gatgcaacga cgattgaaca ggaaaaaagg acaaatcctt ttttcaatcg ttaaaacgac
     6301 ttaggaggag aataaaatga cagaaactac actttatatc gtacgacacg gaaaaacgat
     6361 gttcaataca attgaacgag tacaaggctg gtgtgataca cccctcacaa agcaaggaca
     6421 agaaggcatt cattatttag gtcgcgggtt aaaaaatgtc gacttttcat ttgcttattc
     6481 tagtgacagt ggtcgtgcaa tcgaaactgc ccgtattatt ctaggagaac attctaaggg
     6541 aaaagaaatt ccttatttta ttgaaaaaag gattagagaa tggtgtttcg ggtctcttga
     6601 aggtggatat gacatggaaa tgtggggtgt gatccctcgt gtattgaatt ttaaaacata
     6661 tgatgaaatg tttaccacag aagtttcttt tgaacaaatt gctaatgctg tatacaactt
     6721 agataccgct gggtgggcgg aaacttatga aacattgcga gaccgtgtat ggacaggatt
     6781 tgaagatatc gcccatcact gcgaaaaaaa tggtggtgga aatgttttag tcgtatctca
     6841 tggtttgacg atcgcctttt tgcttaatct gatcgatcct aaacagcctg ttcgtgctgg
     6901 gcttttaaat ggcagcgtaa caaaggtcac ttatgcaaac ggtcgatttt ctatccacgg
     6961 catcaatgat acgtcttata tcgataacgg gaaaacacat agtcgcgact cgtttctata
     7021 atggggatat tttaattttt accagagaat cataaaaggc aaagtctgaa cgagggcttt
     7081 gtcttctttt ttctcttcgt gtacttatct gctttatttc tcaagttttt cttaatatta
     7141 aaaaacagat tgggtttctc aaaaaataac ggtatactat aagatgtatc ttaattggga
     7201 aggaaggaat ttatgttttt tgcaaatatt atcaaagcag tgattctcgg tattattgaa
     7261 ggaataacgg aatggctgcc catcagtagt actggtcatt tgatcctagc agatgaattc
     7321 atcaaactgg atgccagttc acaatttatg tcgatgttca atgtcgttat ccaattaggg
     7381 gcgatcttgg ctgtcgtcgt tctttatttt cacaagttaa acccattttc tccaagtaaa
     7441 tcaacggtag aaaaaaaaga tacgtggaca ctttggttta aagtgatcgt tgcttgcata
     7501 ccagccatca tcattggttt gcctttggat gattggttag aagcaaattt ccacaagttc
     7561 ttaccagttg ccattgtttt gatcgtttac ggtattgcct ttattattgt cgaaaaacgc
     7621 aacaaaaaca aaacacctcg ttggacttct ctgaatgact ttacgtacca agcagctctt
     7681 cttgttgggt tattccaagt cttggcactt gttcccggaa catcccgttc tggcgcaacg
     7741 atcctaggat ctatcttgat tggtgcttcg cgttttgtcg ctacagagtt ttcatttttc
     7801 ttagggattc ctgtcatgtt tggcgcaagc ggcttgaaga tcgtcaaata tttagctgaa
     7861 gggaattcat tcaaaatgga agaaactgtt gttcttcttg taggaacatt cgtatctttt
     7921 gtggtttccg tcttagctat caaattctta ctaagctatt tgaagaaaaa cgactttacc
     7981 gcttttggct ggtatcgtat tatccttggt atcattttga ttggttattg gtttattgca
     8041 atgtagaaaa aaccagaaca cttctctt
//
LOCUS       DADOOS010000119         7998 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.119, whole genome shotgun sequence.
ACCESSION   DADOOS010000119 DADOOS010000000
VERSION     DADOOS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.119"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            260..2026
                     /locus_tag="J8C37_002251"
     CDS             260..2026
                     /locus_tag="J8C37_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAZ5305672.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2128..3387
                     /locus_tag="J8C37_002252"
     CDS             2128..3387
                     /locus_tag="J8C37_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAZ5305673.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3534..4835
                     /locus_tag="J8C37_002253"
     CDS             3534..4835
                     /locus_tag="J8C37_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5305674.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4838..5689
                     /locus_tag="J8C37_002254"
     CDS             4838..5689
                     /locus_tag="J8C37_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5305675.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5702..6517
                     /locus_tag="J8C37_002255"
     CDS             5702..6517
                     /locus_tag="J8C37_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAZ5305676.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6668..7834
                     /locus_tag="J8C37_002256"
     CDS             6668..7834
                     /locus_tag="J8C37_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ5305677.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2609 a         1378 c         1591 g         2420 t
ORIGIN      
        1 cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct
       61 taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt aaaagtttta
      121 agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat
      181 aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga cgtcatatta
      241 aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata
      301 tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat
      361 caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa aatggtatca
      421 aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga
      481 agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg aagataacac
      541 ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc
      601 aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg
      661 ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc
      721 aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttttt
      781 cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc ttggcatcaa
      841 tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga
      901 ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga
      961 agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca
     1021 ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt
     1081 ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag aaactgctga
     1141 aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa
     1201 tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat
     1261 tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag
     1321 agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc
     1381 tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat ttacagattc
     1441 gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa
     1501 tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc
     1561 tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt
     1621 gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg
     1681 gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca
     1741 agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa
     1801 gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag ataagctcta
     1861 tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc
     1921 ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga
     1981 cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt
     2041 aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc
     2101 acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta
     2161 gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca
     2221 gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa
     2281 gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta
     2341 gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag
     2401 ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat
     2461 ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg
     2521 acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat
     2581 aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa
     2641 gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg
     2701 actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat
     2761 gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct
     2821 tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca
     2881 ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca
     2941 tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact
     3001 ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat
     3061 tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat
     3121 caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca
     3181 gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa
     3241 gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc
     3301 gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc
     3361 agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat
     3421 ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt
     3481 gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc
     3541 aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga
     3601 tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc
     3661 tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg
     3721 gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca
     3781 tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag
     3841 cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata
     3901 taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta
     3961 cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc
     4021 atattccacc agccagttta attgattggg taggggctaa aaactttttt agtatcttct
     4081 ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt
     4141 tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga
     4201 aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag
     4261 cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata
     4321 ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga
     4381 aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc
     4441 cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag
     4501 cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg
     4561 tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct
     4621 ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga gctggttcta
     4681 ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta
     4741 tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat
     4801 ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc
     4861 tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt
     4921 cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc
     4981 agcttttagt ttagatcttt caagtgactg gactttagga aactttactc ggctattcag
     5041 tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat
     5101 cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg
     5161 ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt
     5221 gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac
     5281 aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga
     5341 cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat
     5401 ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc
     5461 ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa
     5521 tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc
     5581 tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt
     5641 acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat
     5701 catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc
     5761 gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt
     5821 gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca
     5881 atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga
     5941 attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt
     6001 ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga
     6061 gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa
     6121 ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt
     6181 agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct
     6241 tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg
     6301 gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat
     6361 gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga
     6421 ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt
     6481 tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac
     6541 aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa
     6601 cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag
     6661 gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc
     6721 tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc
     6781 ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcttc
     6841 tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc
     6901 attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa
     6961 atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca
     7021 ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg
     7081 tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca
     7141 tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg
     7201 gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact
     7261 aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa
     7321 atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac
     7381 aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt
     7441 aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg
     7501 cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt
     7561 tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt
     7621 acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga
     7681 tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc
     7741 ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc
     7801 tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac
     7861 tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct
     7921 aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc
     7981 tttaattttt aaacatga
//
LOCUS       DADOOS010000120         7850 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.120, whole genome shotgun sequence.
ACCESSION   DADOOS010000120 DADOOS010000000
VERSION     DADOOS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.120"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(110..631)
                     /locus_tag="J8C37_002257"
     CDS             complement(110..631)
                     /locus_tag="J8C37_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAZ5305678.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(633..1667)
                     /gene="sppA"
                     /locus_tag="J8C37_002258"
     CDS             complement(633..1667)
                     /gene="sppA"
                     /locus_tag="J8C37_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAZ5305679.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(1700..3115)
                     /locus_tag="J8C37_002259"
     CDS             complement(1700..3115)
                     /locus_tag="J8C37_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAZ5305680.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3084..5237)
                     /locus_tag="J8C37_002260"
     CDS             complement(3084..5237)
                     /locus_tag="J8C37_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAZ5305681.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5495..5731
                     /locus_tag="J8C37_002261"
     CDS             5495..5731
                     /locus_tag="J8C37_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAZ5305682.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            5750..5965
                     /locus_tag="J8C37_002262"
     CDS             5750..5965
                     /locus_tag="J8C37_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAZ5305683.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(6668..7420)
                     /locus_tag="J8C37_002263"
     CDS             complement(6668..7420)
                     /locus_tag="J8C37_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5305684.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7435..>7850)
                     /locus_tag="J8C37_002264"
     CDS             complement(7435..>7850)
                     /locus_tag="J8C37_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5305685.1"
                     /translation="LKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGII
                     LDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQG
                     LGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT         2488 a         1416 c         1146 g         2800 t
ORIGIN      
        1 gttgaatcaa tagaagatag aaacatacca cccatcaatg ctaaaagact acataaaatc
       61 cctgttattc cactaattaa aatcaaaata aactctgttt ttctcttatt catttaattc
      121 tctccagatt aagtgtttta gaattagaaa ttttttgacc atagttaata gcttctaatg
      181 aacgaataac aaccactgac gtgttagaaa aataatcccc aatatgttgt ttcttgggcg
      241 tgaaagcagc caccaaatac ccaatttgaa atacaaaatt ttttaatata aacctacata
      301 tacactcacg aataaagata gtttgccaac ttagctcttt ttcctcaaag ctcactactc
      361 ttatcccaaa aatcattttc ccaattgttt gtcctgaatt cagcttagtc aaaagtacaa
      421 aataggttaa ataaatagct aatgacgcta taccataaaa agtaataatg gatttgtcag
      481 attccagtcc catgattttg taaaagagac ctatagtagc atgggtgatg atgctaatac
      541 aaattaaatc aataagaaaa gcaaaaaatc gtaaccaaaa tcccgcaaat acataattag
      601 gcaaagtttc aatgccactc ttcttattca ttttatagcc ctccatagta atacattggt
      661 tttggagtat cagaaaatcc aagtttttca aaaactgaaa ttacacgatc tgatgtagtt
      721 gcctttagcc cttgccattc agcaatctta ttacctaacc aagaagaagc aaagccagtt
      781 gtgtcatttt tatattctat aaccgtcgca tcagttaatt tcttctcttt ttttagggaa
      841 tctaatgcat cttcagagta accaatttga tccaccaacc cattttctac tgcttggtta
      901 ccatcataaa ttcttccatc cgcaagttct ttcactgcat tagtatccat atttcttcct
      961 tttgctacaa cattgacaaa acgatcataa gcactcatca caaattcctg tataacttta
     1021 ttctcttcct ctgaaggttt gtgttgaggt gacatcatat cttttaatgc accactttta
     1081 tacgtcgcat cagttacgcc aagtttttct aataagccag aataatttaa accagaaata
     1141 atgacaccaa ttgatccagt tgttgtttct tccgtagcaa atattttatc tgaaccagca
     1201 gaaatgtagt accctccact tgccgctgta tttttaaatg cagtatagat aggaatatct
     1261 agctttttaa tttttgccat ttctttagca atctcagcac tttcatatat tcctccccca
     1321 ggtgaattta cttctaataa cactccctta accgctttgt cttcttgaat tttctttagc
     1381 tgtgttaaaa aattctgatg gttatattgt tccctactaa aaagattgct ttcacctgta
     1441 tctgcaataa ctccattgac ggatagtttg acaatctttt tatttgatgc gccttcttct
     1501 aacgtttctt ctgtgatttc gttagatccg taaaaaatct tatttattcc tgttagggtt
     1561 gtatttgatg cttcctcttt atcttttcct ggaattagcg cgacgataat ggatagtaac
     1621 aataatccgc acgctactcc cacagcaatc catcttcttt tattcatata aactcctcct
     1681 atattcttag taaaaataat tattttttat ttagaaacac atcgacaatt tgttgccaat
     1741 atgttttttc tcctacaatt aaagacaact ctccagttaa tccataacga aacggaaacg
     1801 attgagttgt ttttaacgtt cctttcactg cataaaaaac acctttctca gtggcagtag
     1861 aattttctga aatttcatct aaagttccgt cgatcgtaat cggagcccca cctttttgat
     1921 ccaatttaaa atgaaccttc atcccttctt taatataggt actttcattc gcaggtaaaa
     1981 gtgctgtaaa tttcattttt gatttttcgt ggatcggata aatttctgca agcactgttc
     2041 ctttggctag ttctgcttgt ccctttgttt cttcatttaa atgaaccgtt ccagatacag
     2101 gagcttttag tattcctttt gaaagttgat cgtccaaact tttgataact gactggttct
     2161 tctcttgttt ttcatttagc tctgtaactg tttgttttac agttgttatt gcttgttcta
     2221 gctcttgctt tcctttttcc gtttgactaa ttctgtcatt ctcagaagta gttggtttaa
     2281 ctaatttagc tttttctgac tgatactgtt ctacttcttt ttttagttgc tcaatttgtt
     2341 catttatgct tgctgaaatt gtcagtttca cttgattttt ttgatcatca gaaacattat
     2401 ttagttgttc ttgccaattc tcatagtttg ccatcatttc ttttgaaaaa tcttttaatg
     2461 attgattatt actccaagct acctgtactt gttgccattc ttgtaattta ctatctgaac
     2521 tctcaatttg tttttctagt tgtctttttg tcttttcgta tacttctaat tgcttttgat
     2581 catttaattc actttgcttc aaagcgtagc ggagtgattc tttttctgac aacatgctct
     2641 ttaattgatt actgtagcca aaactgtcat ccgttgaaaa caaatttttt cctttactta
     2701 tactttcaat aaatagctga gccatcttta tttgttgagt aatacgttgg ttttcttgtt
     2761 ctatctgagc cttttcattc tctattaaag aacaatccaa tctaaccaac acatcgtcct
     2821 tatgaacaaa ttgattttct tttaggaagt tttccattat tttagcttct aatggcactt
     2881 gtaacttctc tattttttcg gggttcaatt gagccgttgt ccgaataaca acctcttttt
     2941 tagcaaaggt taaaaacaat ccaataatac ataaaaaaag aattacaggg tataacaccc
     3001 aacggtagaa gctatgatgt tgtttagtat agatactaga atgttcgaac caattattat
     3061 tctgcataaa ttgtttccct ctcctagatt tcccataatt tttgatagat accatcggca
     3121 aaacgaagtt catcgtgcgt tcctttttct accaaatgcc cttcatggag aactaaaatt
     3181 tgatcacatg cttttgctat tggtaaatga tgcgcaataa aaataatcgt tttttctttt
     3241 aaattgagga gataattcat aattgcatgt tctaacaagg tatctaatcc actagttgct
     3301 tcatctaaaa ttaatatgtc cggatttttt aataaagccc gtgctaaggc taatcgttgt
     3361 cgttgccctc ctgaaagatt atttccacct tcttccaaca atgtctcgta acgtaatggc
     3421 tgttgattaa taaattcatt tagttggact atctcacaaa cttctattat ttgttcgaaa
     3481 cttggtgggc tatctaaacc gaatataaga ttctcaatga ttgttccact aaagaaaaat
     3541 gactcttgag gcaaatatgt cactcgatcg cgtaatatct gaaaaggaat gtctaagtga
     3601 ttaatgtttc cataatggat cgatccttct gatgcttcat aaaaacgaac aaataacttt
     3661 gcaagagttg attttccaga accactaact ccaactatag ccgttttact gtgcgaagga
     3721 attgtacatg aaatttcgtt caacgttggg gccttcatgc tatacgagaa tgaaacgttt
     3781 tttacagtga tatcttgttg aaataattgt tcagaaagca tatattcaaa agactggttc
     3841 gatttttctg aatcaatcgc gaaaatttca tttaaacgat tatttgcaac ttgagccgtt
     3901 tgcattttca cttgtaaatt aataatgttt tgtaaaggat ctgtcaaaaa tactagtaaa
     3961 gcattgtatg tgataagttg acctagacta attctattat ccataacaaa ataagaacct
     4021 agccataaaa ttaatgcact gctaattaat tgaatcaaat gtttaattcc ttgttgaata
     4081 ttatcaagcg taaccgtacg aaatgatttt ttcattaatt taacaaattc atggtctata
     4141 cggttatata ctttctcttc accgctatat gctttaattg tttctattcc tttaagactt
     4201 tctatgatac tagaatttac agctgcaccg gctgccattt cttccctgtt cgatttttca
     4261 tagcttttca cgaaagcgaa tattgcaact acataaaaag gcaaagagca tagagtgatt
     4321 aaaaacaacg tactattctg aatagctaac gtaaccccaa caagcaacac cataccaata
     4381 tctaaaaata ttgataacgt agcacttgct aaagcatcaa taatcttact ggcatcaaga
     4441 aaacgagaaa taatttcccc tgattttctc gtagcaaaaa aattcattgg taaagttaac
     4501 acatgtttga aatactgaag cataattgcc atactcattc gctgtcctaa aataataaga
     4561 agataactac gactatattc aaacaatacc cgaaataaat agacagcgat caagccaatg
     4621 gaaattatat ttaaagttga acgtgcttga ttagggatga aaaaatcaag aataccttga
     4681 aaataatatg agcttcctat gccaaaaaac gtaatgaaaa tcgatgctaa aacgatgtgg
     4741 aatactaatc ctttttgttt ccaaattata ggcaaaaaag aacttaatcc agctactttt
     4801 tctttaatag gaatataagt ttctctaggc ataggtaata aaagaatacc tgtccattct
     4861 ttttgaaacg cttcaatcgt ttttctattt tttccattag cgggatctgc aatccatagt
     4921 acgtttccct tcgttttata aattacaacg tagtgcatat agctgttatc aaccaatata
     4981 tgtgcaatta atggtaagat aagatctctt tctttccaga tctcactatc tgcttgaata
     5041 gccatacaat caaaagacaa tttttcaaat gtctttttca atccaaatgc tgaagtaccc
     5101 tctaaatcag tcccagatag gtcccgtaat ttatgaatcg ggatatcagt tttaaaatat
     5161 tttaaaatca tcgaaaggca agcaacacca caatcttttt catcttgttg ctgcacaaaa
     5221 ggaaatgatt ttatcactta tctaacacaa ctccttctaa aaaattttta tagaaaaatt
     5281 ctcaatatag ctttaataca gtgaaaatat tgtctcataa ttccacaaaa actttgaaaa
     5341 cagttcttga acactaactt tttcattcct gaatcaaatt tattttggaa attttactat
     5401 tcaagaataa acttgataag tttcaggaag atttttactg ttaatcaaat acgtatgtta
     5461 cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt aaacatgcaa aatgtaaagg
     5521 aactaagtat tgtggaaatg cacaaactgt tggtggaggc aataatgctt ggggaaaatt
     5581 ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt ggtgcaggtt taggaacagc
     5641 aatttgcgga ccagcttgcg gtgttgttgg agggttatat ggagctgtcg ctggaggtgc
     5701 tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga ataagtagta tgcatattaa
     5761 aaacacaaaa acaacattta tattatcttc agaagagttg aaaaatattc aaggtgggag
     5821 tgccgtggga gttttaggca ctactttttc tggggcaaca gcaggagtaa aactttgttc
     5881 agccggagga ccttatgcaa tagctgcttg tggtgtaggt ggagctttat taggtgctgg
     5941 attcagcatg tggaccggtg cgtaaacaat taaaaaatat caaacctcaa gattggtttg
     6001 tgtcgattac gaacataggt atgttcttta tcttttcaaa agtttggaat ggacagaact
     6061 ttttactaaa gcttcttgtg ctagttttta taattatatt aaatttttat cttttattaa
     6121 agtcagataa aaattctttg gaaatgtaat ggtagagata tttttacaaa aggacttgca
     6181 gcgctgtagt tggtgctgca ggaggaccta tttcggccgt tacaggaggt attggtaatg
     6241 tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta aatttataaa aacaggaaag
     6301 taattatctc aaatcctatc aagtacaatc ataggattgc acttgacagg acaaattttt
     6361 taactaaaaa atgaaaaaca ctttctaaaa attagtagaa tttgccaaat ctttcgcatc
     6421 ttccaccata catttaattt taattcccct ttatggtact taataaaaac aagaaaagaa
     6481 ctattcattt ggcccttttt tggttccaga ccatagaaat attcctccag taactaatat
     6541 tgctgttatg cacgtaacta ataacagcta taaaaattgg tacaacttta ataggattca
     6601 gaaaaatttg cactagtgaa ggaatctatt ttattagaaa aatgcaatct ccctaaagaa
     6661 aaaaccacta aacagtgctt tctttcaact tctccttaat tttttgtgct ttccctaaag
     6721 taaagtttct ggccaactct gaatcaaaat atagtgaacg ttttttaaaa tctatttctt
     6781 taacgttaaa gatatttact aaacacgctc gactaattct tgttaacata ggatatttct
     6841 tttcaagctc actaattctt ccataaaatt cgtactgccc atctttagta cacaatgata
     6901 atctatgtgg taattttgaa gcttctacaa aataaatgtc atttatatca aaagtaaatg
     6961 tttgagaacc tatggaaaaa acaaatgctt gattcgtaac ctgttttgtt gcatctatcc
     7021 tttcttgcgc taataacaat aactcaacaa tctcgtttcg atactcatct agtgtttgat
     7081 cttttgttac aaatcctaac gtttcaactc ttctttttat tgttacgggt aacatctcat
     7141 catgagtagt tgtaaaaatg atttttgctt gaacatcata ttttctgatt acttctgcta
     7201 attctatacc gttaacttca tgatttaaat caatatctaa gaaatatatt ccttgctttg
     7261 gtcggaattg tttgaggtat tttttaactt ctaacggact ttgtgttttc aatactatct
     7321 taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt
     7381 gtataatttg atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa
     7441 caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt
     7501 gggattcttt ttattgattt cctctatact agataaacct aacccttgat gtcccttttt
     7561 tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt
     7621 cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc
     7681 ttgactctct atagatgctt caattgcatt atcgagaata attccaagca ccctaataca
     7741 gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat
     7801 cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat
//
LOCUS       DADOOS010000121         7836 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.121, whole genome shotgun sequence.
ACCESSION   DADOOS010000121 DADOOS010000000
VERSION     DADOOS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.121"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(22..996)
                     /locus_tag="J8C37_002265"
     CDS             complement(22..996)
                     /locus_tag="J8C37_002265"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAZ5305686.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="J8C37_002266"
     CDS             complement(1076..1951)
                     /locus_tag="J8C37_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAZ5305687.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="J8C37_002267"
     CDS             2081..3988
                     /locus_tag="J8C37_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAZ5305688.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="J8C37_002268"
     CDS             complement(4529..7807)
                     /locus_tag="J8C37_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAZ5305689.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVVMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2209 a         1658 c         1460 g         2509 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatcact acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaag
//
LOCUS       DADOOS010000122         7794 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.122, whole genome shotgun sequence.
ACCESSION   DADOOS010000122 DADOOS010000000
VERSION     DADOOS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.122"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(9..1013)
                     /locus_tag="J8C37_002269"
     CDS             complement(9..1013)
                     /locus_tag="J8C37_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAZ5305690.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(1010..1999)
                     /locus_tag="J8C37_002270"
     CDS             complement(1010..1999)
                     /locus_tag="J8C37_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAZ5305691.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(1996..2802)
                     /locus_tag="J8C37_002271"
     CDS             complement(1996..2802)
                     /locus_tag="J8C37_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305692.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            2968..3924
                     /locus_tag="J8C37_002272"
     CDS             2968..3924
                     /locus_tag="J8C37_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5305693.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(4001..4519)
                     /locus_tag="J8C37_002273"
     CDS             complement(4001..4519)
                     /locus_tag="J8C37_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ5305694.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(4607..4756)
                     /gene="rpmG"
                     /locus_tag="J8C37_002274"
     CDS             complement(4607..4756)
                     /gene="rpmG"
                     /locus_tag="J8C37_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAZ5305695.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4984..5430
                     /locus_tag="J8C37_002275"
     CDS             4984..5430
                     /locus_tag="J8C37_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAZ5305696.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(5625..7520)
                     /gene="asnB"
                     /locus_tag="J8C37_002276"
     CDS             complement(5625..7520)
                     /gene="asnB"
                     /locus_tag="J8C37_002276"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAZ5305697.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         2522 a         1604 c         1364 g         2304 t
ORIGIN      
        1 aatgaatttc aactgttcag cgcgtgtttt tgtctgataa aaagtgcata cagaaaatat
       61 ggcgcaccaa aaagggcaat caaaattcct gtaggtactt ccccacttgg tagaatcact
      121 ctagcaacca tatctgcact aagcagaaaa atggaaccga acaaaccgct cagaacaaag
      181 gtccagttat tttctttacg tacgactagc ttcgcagcat gaggagctag tagtcctaca
      241 aaagataaac tacctgtcac cgctacacta ccgcttgcca aagcaacagc taacaacaaa
      301 aggatttttt gacttttagc cacgttaagg cctaatcctt gcgcgcgttc ttgaccaaat
      361 gatagcaata caatcctctg tgcataaagt cctcctagcg gaattaagat aaccgcccat
      421 ggaagcaatg cccaaacata aggccagctc gcaccccaga ttgatcccgc taaccatgca
      481 ttcacaaatc cataattttc cttagataat tggatcgtgc atagcaatgt taatgcagaa
      541 aggccggcat taacggcaat accggacaat aagattcgat tcatacccaa aaagccattt
      601 cgatcatagg ccgcaagata gacacagcaa gcagctaaaa caccacctag aaaggctaca
      661 actagcaata gaaacggcag cgaatagtgc gaaaagaaac ctaaataaag catcactaaa
      721 aatcctgctc cggcattaat gcccaatata ctagaatctg ccaattcatt tcttgttact
      781 ccttgcaaga tataaccaga aacgccaaga caagctcctg ctaataaact gatcagtaat
      841 cgaggaactc gaaattctct aacaatcagc gccaaagaag atgattcttt ccctatgaac
      901 gtggcaaaaa gctgatccag gtaaataaaa ggcgtaccaa tcattaaact acctatagac
      961 aggatgacaa gtacaaaaag caagaaatag aacgtccaat atattttttt caccgttgtt
     1021 ccctccttac ttgtatcagt aaaaacggaa cgccaatcaa tgcaatgact aatccaaaag
     1081 gtgtttcaaa agggggattg atcatcctcg ctgtaaaatc cgccaacata accaatccac
     1141 cgccaactaa aaaagagaca ggtagaacat aacgatagtc cgttccaacc attccgcgaa
     1201 caactggcgg aaccaacagc ccaataaatg aaacaggtcc gactaacgaa acggcaatgg
     1261 ctgctaacac tgcaactaga cagaaaacaa tcaaccgcgt tcgtttagga tgacttccaa
     1321 gagctaatgc atgtgtttca ctcaaatgaa tcaaagatat tgcttttcct aatgaaaaag
     1381 aaaccaaaac agcaagtata tataaaggaa acaatactgc taattgtttc catgtgatat
     1441 tggcagctcc gcctacaaac caaaaagcaa gatcctgctg cagattaaag aattgggtaa
     1501 gtgcttggct gatagcagta aaaaaagaac taatagctac gccagctaaa atcagctgta
     1561 aaggattcat gcccattttc gaaaactgca aaaaaccaaa tacgagcaaa gttacagcaa
     1621 aagaacctgc caatgacatt agaaacacag aaaaatcaga agctttaggc caaaaagcaa
     1681 aactaagggc cattgccaac gaagcacccg cgttgattcc tagtaagcct gaatctgcta
     1741 aaggatttct tgtcacccct tgcatcaatg caccgctcaa tgcaaaacaa ctccctacaa
     1801 gaaatgctcc cgccacacga ggcaaacgaa tatttcgtat gatttggtga ctttgttttg
     1861 atctatcaaa atcaaacaac gcctgccaaa aatctgcttt ttccacagtc gctgcgccat
     1921 ttgaaagagc aacaaaaaaa ataatcaaaa gaacaaaaca gaccaaaagc aggagtctat
     1981 ttttcttcat agtcctcata attgatcctc ctgctctttt ggcaacaaat cataggttag
     2041 aaaaactggt ttatttgttc ttggaagcgt gacaatcgtt gcatcaatct caaatatttt
     2101 tttcatacac tcttttgtga tcacttcttc aggtgtacct tctgtgatca acttaccgtt
     2161 tttcattgct aataaataat ctgaaaaacg tgaagcatga ttaatgtcat gaatcgacat
     2221 caagatcgtt gtttgtttca tttgattgat ttcagtcaat aactgtagga tctctaattg
     2281 gtgtgccgga tctaaaaatg ttgtcggttc atccaagatc aagatatctg tttcctgagc
     2341 aagcgccata gcgatccaga cacgctgggt ttgtccacca gacagcgttg cgatatcccg
     2401 catacgaaat ccagctagac ctgtagcatc caacgcccac tcaattgctt catgatcttg
     2461 cgtgtccatg ccactgaaca gtttttgata agggtatcgt ccgtaagata ctacttcttc
     2521 tactgtcata cctgtagggt gttcactagt ttgtgctaat aatgcaattt tttttgcaag
     2581 ttcttttgaa ctgtatgtag agatatcaga gttattgaca atgatctttc ctgatttcac
     2641 aggtagtata cgggtaaaac ttttcaataa agtagatttt ccacttccat ttggcccaat
     2701 caaactagtg attttccctt caggaatcgt aacagacatg ttttcaatga tttttttgtt
     2761 ttcgtagccg gctgcaatgt cagctgctct taatctcgtc atttcaccca tcctttcgct
     2821 gaaaatcatt ctcaattatg acgctgtttc aacatttcgt caagcgctac tttcatatct
     2881 atagccaaat gaagcggttg acagcgttcg aaatatcagg aataataacc aatgagaaag
     2941 attctcaatt atgttaggag gaaagaaatg aaattaaaaa caaaagtatt cacattatct
     3001 agcatattgc tgctggctgg agtactgagc gcttgctccc cctcttcaga taaagaaaca
     3061 gctacaactg ccaattcgac gacttcatcc aatagtactc atacagtcac agatacactt
     3121 ggacataaag tagagattcc aaacgcgccc aaacggatca tcggcagtta cttagaagac
     3181 tatctgattg cactagggga aaaacctatc gcccagtgga ctgttggcag tggttccatt
     3241 caagattatc tgcaagatga tttaaaggat gtcccaacga tttcttatga ccttccttat
     3301 gagaaagtgt taagctttga gcctgatttg ctgttgattt cttcttccgc tactgtcgaa
     3361 ggcgggaaat acgaacaata tagcaaaatc gcgccaactt atgttgtaaa aaacggaaca
     3421 gatgtcacat gggaagaaca actaaaagat gtcggcaaag ctttggcaaa agaaaaagag
     3481 gcagaaaaaa tcatttccga ttatcaaaaa tccatcaaga aaactcgaga agaactagca
     3541 gataaaatcg agaacaagac agctgcagtg ttgtgggtga ccaacaactc agcctttatg
     3601 gtcagcaaaa atcggtcaag tggaagaatc gtatacgacg atctggaatt tggtgttcct
     3661 gatctagtgg aagaggtctc caaaaaagcc acttctgact ggtctgctgt ttcctctgaa
     3721 aagctagctg aattagatgc tgactacatc atccttgtga acagtgacaa agatgctgcc
     3781 atgttcaatg aaccaagctg gaagaatcta aaagcagtga cagacaatca tgtactgcaa
     3841 ttcggacctg aatctagttg gctatataac ggacctattg ccagtcagga aatggtcaat
     3901 gatatcaaag aaaaactaaa ttgattttaa ctaaaaaaag catcttcacg attagtcgtg
     3961 agatgctttt tttcttatta acaaactaga cgcatagatt ttattcttct tttgaagcaa
     4021 ccctattttt tactctttcc catttttgaa aacgatgggc atatagattt tcttgatccg
     4081 ttatcccctc atactcctct ataagggcga agtctgaaaa atcaatattc ccaatatacg
     4141 tatcgccttc aaacgtgcta tcgatcaatg ttcgccagat ccgaccagtg taaggaagaa
     4201 gttcttgaaa aatccgacta ccacccgaaa catagatatc ttctgagatc gtttgactat
     4261 acgacaaaag ttcatcgatc gaatgcatta cttccgcatt ttctttcgtg cgaaaatttg
     4321 gttgcgttgt caacacgatg atatggcgat aaggaagtga aagatccccc attccttcat
     4381 aggtctttct tcccatcact aaaattcgat ccattgtatg ttcgcgaaag aaacgcatat
     4441 catttggcaa tcgccatggt aacaaaccat ttttgccgat cagaccattt ttatcttgtg
     4501 cccacatgga aataaacata ttatccgctc cttatgattt tagatgtttt gagataaaaa
     4561 aagtctcaat tctttgacca gcaaggaatt gagaacttta aaaacattat tttgtttcac
     4621 ggtgcaaagt aactttgcgt tcgcgtgggc aatatttttg tttttccaaa cgatcaggat
     4681 tgttacgttt gtttttgctt gttagatagt tacgttcttt acaagaagta cactctaaag
     4741 tgatgtttac gcgcatgttt ttccctccca tttttacaag aatcctctaa gatgactctc
     4801 agacttgatt atcttaccat gattttttct agattgctag tgtttttcac gattttgtta
     4861 agtaaaacta cttgacgata aatacaacgg ttttacttct caaacaaaat ctttttcttt
     4921 ttgtataaat acccttacac ttgtttatat agattatgtt ttttaataaa gggggatact
     4981 aatatgccat ggaacatgaa ggactttcct gcatcaatga aaaacttaga taaattgaca
     5041 agaaaaaaag caatcgacat cgcaaatgca ttattagatg aagggtaccc tgataaccgt
     5101 gcgatcccaa ttgccattga tcaagcaaaa aaatggtatg aagatgctag tgaatcagaa
     5161 aaacgaactt tcgaaaaaga aaaaaatcca agcaagacgg atgaacacga tacgaatcct
     5221 cgtgctggaa aactgcttga ttcagatgtg atcgtagaat atgaggaaga acaatggatc
     5281 gtgaaatcta aaggggcaaa aaaggcaagc aaccattttg atacaaaaaa agaagcaatc
     5341 gaaaaaggaa agcaagtagc acaaaacaaa gaatcaactt tgttcattta taaaaaagat
     5401 gggacaaaag aaaaagaaat cgattattga acatatgtaa aggggttgtg acgaaaatcg
     5461 tgtcacaacc cccttatgtt cgaaacaatg gtgttcctct gccaactccg aaccataagt
     5521 cataatatct ataaaacggg aagagctgtt ttttgaaatt gtttcttatc actcagtgtt
     5581 aaacggtagg agaaaacctc ttttttattg atatttgatt ttcatcaaac agtcacatag
     5641 tcgattgctt ctgcttgcgg aatcgtttca tctacaaagt agactttata ccatgtcaag
     5701 aaactgaaga ttgtccagat ttttctttgc tcttctttac gtccttcgtg atggtcatct
     5761 agcaacttca tgattttttc ttgatcaaaa aattcttttg caaaatctgc ttcgaataat
     5821 gctttgactt gttcgtagcc gtgttcttct ttcaaccaag ctttgattgg cacagggaat
     5881 cctaatttca cacgattcga ccattcttcc ggaagatgtt tgttcgcagc ttgtctgaaa
     5941 atatcttttg tgttgttttg attcaataaa aacttacttg gtatttgttg agccaatttc
     6001 atgacttcga tatccaataa aggtactcgg acttccaaag aacttgccat agaaagttta
     6061 tctgccttta acaagatatc ttttggcatc cattgatgaa gatcgacata ttgcatctta
     6121 ttgacttcat ctttgattcc ttctgttttc tcgtaatgaa cagtcatgat ttcatccaca
     6181 gatggtgctg tttgatattt tggtgttaac acttctgatg cttctgattc ttcgaacact
     6241 cgagcatgac cgataaaata ttcttttgca ggagcaagtg attcatataa atggatccgt
     6301 ccatggaagt ttttcatttt tccgatttta cgtcctaaat tatagcgtgt tccttttggt
     6361 aattttttca atccttgagc aatcacacgg atgaattttg aattcgtatg gaatccatac
     6421 gtttggtatc ccgcaaacag ttcatccgcc ccttctccag attggactac ccgcacactt
     6481 tgtgaagcaa gatttgctaa aaagtacaaa ggtacgcaag aaggatttga atcaggttca
     6541 tctaagtgat actggatcaa agggaatgct ttgaacgaca tgtctccgtc gatcactgct
     6601 gctgtattat ctaagtccaa tagttcggtt agttttctgg cttcaatcgc ttcattatag
     6661 gttttatcat caaacccgat tgagaaagaa tgatctggtt tcaatacaga ggttacataa
     6721 ctggaatcaa ccccgctgga taagaaagaa ccaacttcta catcactgac cgtatgtgct
     6781 tcaatagaag cttgaacggt ttcgtcgatt ttttcgatcc attcttgtcg ggaatgcgtt
     6841 tcttttgttt caaaatctgc atcccaatat tgctgaatgt ctatttttcc atctttatac
     6901 gtataataat ggccttcagg caaacgatag acatctttga agaatgtttc gccgtttaat
     6961 ggagaatatt ggaaagtcat ataaggtttc aacgcttctt tgttcaattc tttgttaaaa
     7021 tcaggatgag gcagaaaact tttgatttct gaaccaaaca taaatgttcc gttcatttct
     7081 gcataataat atggtttgat cccaaagtgg tctcttgcac cgaataactc attcttttca
     7141 ttatcccaaa tagcaaaagc aaacattccg cggatctttt gcaataattc tgttccccat
     7201 tcttcgtaac catgcaaaag gacttctgta tctgcatgtg tttgaaaaac gtggccagct
     7261 gcaatcaatt cttctctcaa aggctggtaa ttatagattt cgccattaaa aataatgatc
     7321 tttgtccgat cttcattgta aataggctga gtaccgccct ctaagtcaat aatacttaat
     7381 cttctgaatc ctaaagcaac atgtttatcg atatattctc ctgaactatt aggtccacgg
     7441 tgaacgattc gatccatcat tgtattaata attgtttttt tgttgtcctt gtcgttaaca
     7501 aaacctacaa taccgcacat ttaactttca catcctaata taaaattact gtttatttca
     7561 ttcatatgca tcttatcata gaataaatag cggacgaata ttttctctct tacaatttcg
     7621 tcacgtgaaa acaaaagatg aaagtttctg tttttacaga aaataaagtc ccaatatcta
     7681 atttaccatg ttttcttttt tgaggctacc ttattctctt cgtgttaaaa aaatattagt
     7741 ggatgctatt tccaaaaaaa ccagcaaaaa aagacacgac ctaaagatcg tgtc
//
LOCUS       DADOOS010000123         7657 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.123, whole genome shotgun sequence.
ACCESSION   DADOOS010000123 DADOOS010000000
VERSION     DADOOS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.123"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(59..787)
                     /locus_tag="J8C37_002277"
     CDS             complement(59..787)
                     /locus_tag="J8C37_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5305698.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="J8C37_002278"
     CDS             complement(800..2080)
                     /locus_tag="J8C37_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5305699.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="J8C37_002279"
     CDS             complement(2221..2823)
                     /locus_tag="J8C37_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAZ5305700.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="J8C37_002280"
     CDS             3068..3274
                     /locus_tag="J8C37_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305701.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="J8C37_002281"
     CDS             3586..4305
                     /locus_tag="J8C37_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAZ5305702.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="J8C37_002282"
     CDS             complement(4386..5069)
                     /locus_tag="J8C37_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAZ5305703.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="J8C37_002283"
     CDS             5287..7473
                     /locus_tag="J8C37_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ5305704.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2740 a         1291 c         1440 g         2186 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
     7621 aagaattttg tcacacctcc cttatttatt ttgaaaa
//
LOCUS       DADOOS010000124         7654 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.124, whole genome shotgun sequence.
ACCESSION   DADOOS010000124 DADOOS010000000
VERSION     DADOOS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.124"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(111..1259)
                     /gene="nagA"
                     /locus_tag="J8C37_002284"
     CDS             complement(111..1259)
                     /gene="nagA"
                     /locus_tag="J8C37_002284"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAZ5305705.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1335..2171)
                     /locus_tag="J8C37_002285"
     CDS             complement(1335..2171)
                     /locus_tag="J8C37_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5305706.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2355..3572)
                     /locus_tag="J8C37_002286"
     CDS             complement(2355..3572)
                     /locus_tag="J8C37_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAZ5305707.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3955..4038
                     /locus_tag="J8C37_002287"
     tRNA            3955..4038
                     /locus_tag="J8C37_002287"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3989..3991,aa:Leu,seq:gag)
     gene            complement(4278..5648)
                     /locus_tag="J8C37_002288"
     CDS             complement(4278..5648)
                     /locus_tag="J8C37_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305708.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6362..6652)
                     /locus_tag="J8C37_002289"
     CDS             complement(6362..6652)
                     /locus_tag="J8C37_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305709.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6860..7552)
                     /locus_tag="J8C37_002290"
     CDS             complement(6860..7552)
                     /locus_tag="J8C37_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAZ5305710.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2332 a         1517 c         1182 g         2623 t
ORIGIN      
        1 aggaaattaa agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa
       61 ccttaaaacc aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg
      121 aaacgttcca cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata
      181 aagtctgcat cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt
      241 gctggaatca gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca
      301 acatttttaa ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga
      361 cgtgctgttc cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc
      421 atcccaccgg ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc
      481 attaggattc ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag
      541 acatgttcta aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc
      601 ataccattga aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct
      661 tctagtgtag cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg
      721 acaaactctt ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct
      781 gaagcttctt gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt
      841 tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta
      901 gctcctggtg cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct
      961 ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca
     1021 gacatcactt tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga
     1081 atatgtgtat ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact
     1141 tctccctcag gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag
     1201 cctgcatgtt tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt
     1261 ctattctctc cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga
     1321 atggtctaca ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca
     1381 tagaatgctg ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat
     1441 aaatcattga ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc
     1501 tgttctaacg cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa
     1561 atttgcgaaa attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt
     1621 cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata
     1681 caactaatca gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag
     1741 cttaatgctt ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg
     1801 caaacaccga gataataata atagcgttga aagtcagttg cttgatacag attttcagaa
     1861 atatcagaag attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta
     1921 tccaacagtt caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca
     1981 tgatcaatcg ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt
     2041 tcctcattat tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt
     2101 ttttgcgtca ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt
     2161 aacgtttcca taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact
     2221 ttaggaagtc gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta
     2281 agaaaactcc actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa
     2341 tttggttttt aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct
     2401 gtttgtttca aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc
     2461 caattaaatc caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg
     2521 aatttttcat catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa
     2581 gcagcttttg gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat
     2641 tctctttgtt catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg
     2701 cctagcgcgg tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac
     2761 aacatattca gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc
     2821 cagcctactc gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg
     2881 aacaaatcag gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga
     2941 tcatagattt cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct
     3001 tctaataatt cttttggata aagtgcccca gtaggattat taggattaat gataacaata
     3061 gcttttgtcc gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc
     3121 tcttcatcgc aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac
     3181 aacggatagt ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata
     3241 cacatcgtga tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc
     3301 ggaaaatgtt tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg
     3361 gaatctgagt agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt
     3421 ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc
     3481 tcttcttgca tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct
     3541 tctaatttgt tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag
     3601 ttagaacttt ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa
     3661 ttttgccact tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac
     3721 ttaattgtca tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat
     3781 gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa
     3841 gatcattccc agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat
     3901 ttttgagctt gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat
     3961 gtgttggaat tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt
     4021 cgagtcccat catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt
     4081 ttgaacgttt ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac
     4141 aatcatgtaa acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac
     4201 ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc
     4261 gagggatttt ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc
     4321 acggttaatc caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct
     4381 ttaactgcca ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt
     4441 ccggaagccc atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc
     4501 atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca
     4561 attaaacata gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa
     4621 agtgacagtt tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc
     4681 attgcccttt tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa
     4741 ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat
     4801 ttcctatatg ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct
     4861 aaaaaaaacg gctttccaaa tgatgaaata cctaaatcta acttattaag aaagttgaaa
     4921 tttttatatt caggaaacca tttattaatt tcatgtgcag cacgtaatgc attccctgga
     4981 gttgtcaaag agaaaattaa ttctgctaag cttattattg aaaatactga taacttataa
     5041 ttatatcgtc cattgacaat caaatataaa ctaactatac ttgttagaac aaaaaaacag
     5101 gcgttgactt gttcttgatt agttaaaaat atcaataaag gaatacttac ccaatatatt
     5161 tttctattta tttcttctcc tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta
     5221 aatgctactg gccactgata gttcaaggtg gttgctctcc aaccggtttc ccccattact
     5281 gtcaatggta ttaaacagta tatagagcaa accaataata aatttttaac atttattttc
     5341 tcaaaaacat acttgcaaag taatactaca attcctaaca taatcgtaga atttaataat
     5401 ttccatagca taaagtgttt agaaaagaac attaagaata gttctataat aattcttgaa
     5461 ctccatgtct tataatgttc tatcgaagca cccaaatagc caagttcatc taatctactt
     5521 gcataaacta cgtcatccgc tctcatagtc ggcatataca tattaaagag taaaaagata
     5581 ccccaaatcc ctattagtaa caagatcagt tctttgttat tcgttatttt tctattatgt
     5641 ttgttcattt ttatactcct tcttatattt cctaatagag tataacatag ctttaagcat
     5701 tacttaaaga taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga
     5761 tgcaaaagaa atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc
     5821 ttagttaagg acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca
     5881 tacaatcata ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc
     5941 caaatgttag gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca
     6001 atttactttt acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa
     6061 cgtttaaaat cctacaattc cactacctga gactagtaat gactagtctc tttttatggc
     6121 ctctgatccc cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta
     6181 agcaatatcc ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca
     6241 tgtagttata tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat
     6301 cgtctgcatc catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt
     6361 tctagtttat tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt
     6421 ctaccacaat tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat
     6481 tattataata acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca
     6541 aactatggga agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg
     6601 cactaacttg caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca
     6661 cctcaatacc attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt
     6721 tttgactttt tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag
     6781 gacatgtact aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag
     6841 gtttacttgt taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat
     6901 cagctgtatc ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc
     6961 ttgagttttc agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta
     7021 catttgtttt ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt
     7081 gtaattctga aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat
     7141 gattgatttc atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt
     7201 tgctttgtag ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg
     7261 ccgattgatt tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg
     7321 cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg
     7381 cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa
     7441 tatctgtctc tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa
     7501 ttagacatag aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag
     7561 ctccttaatc caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg
     7621 tttttaaatt acatgtgtaa ttttaatata aata
//
LOCUS       DADOOS010000125         7547 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.125, whole genome shotgun sequence.
ACCESSION   DADOOS010000125 DADOOS010000000
VERSION     DADOOS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.125"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(229..906)
                     /gene="nth"
                     /locus_tag="J8C37_002291"
     CDS             complement(229..906)
                     /gene="nth"
                     /locus_tag="J8C37_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAZ5305711.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(913..1611)
                     /locus_tag="J8C37_002292"
     CDS             complement(913..1611)
                     /locus_tag="J8C37_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAZ5305712.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1879..3063
                     /locus_tag="J8C37_002293"
     CDS             1879..3063
                     /locus_tag="J8C37_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAZ5305713.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3069..3512)
                     /locus_tag="J8C37_002294"
     CDS             complement(3069..3512)
                     /locus_tag="J8C37_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5305714.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3738..4025)
                     /locus_tag="J8C37_002295"
     CDS             complement(3738..4025)
                     /locus_tag="J8C37_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305715.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4196..5548)
                     /locus_tag="J8C37_002296"
     CDS             complement(4196..5548)
                     /locus_tag="J8C37_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAZ5305716.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5821..6267)
                     /locus_tag="J8C37_002297"
     CDS             complement(5821..6267)
                     /locus_tag="J8C37_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAZ5305717.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6503..7429)
                     /gene="cysK"
                     /locus_tag="J8C37_002298"
     CDS             complement(6503..7429)
                     /gene="cysK"
                     /locus_tag="J8C37_002298"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAZ5305718.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2038 a         1545 c         1240 g         2724 t
ORIGIN      
        1 atactggact tattttatag gtatattgga aggcattatt ttctacatcg ggtattttcc
       61 caataaaaat aacaactttt atttttatcg caataaattg taataagtta ccatcctagc
      121 tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag cttttctcca
      181 taataacaag ataattcata catttatttg caattaaaga aaccacgtct acaggcgtgg
      241 tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga
      301 tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc taccaaaaaa
      361 aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc
      421 ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc
      481 cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc
      541 tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc
      601 atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata
      661 caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc
      721 gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc
      781 ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc
      841 atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct
      901 taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg
      961 ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc
     1021 ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac
     1081 gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc
     1141 cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag
     1201 cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt
     1261 tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg
     1321 gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa
     1381 tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc
     1441 catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg
     1501 tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag
     1561 attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc
     1621 tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta
     1681 tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt
     1741 ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta
     1801 gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt taacattatt
     1861 gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac
     1921 gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta
     1981 acgctgcctt attctcactc catacatcag caagctttct atatcacttt gctcatgtct
     2041 gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt
     2101 gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt
     2161 ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca
     2221 ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc
     2281 aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata
     2341 gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc actctttact
     2401 ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt
     2461 tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag
     2521 atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc agctggctct
     2581 cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt
     2641 ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct
     2701 cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc
     2761 ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt gtttttattt
     2821 gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta
     2881 tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa
     2941 tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt
     3001 cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa
     3061 tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact
     3121 gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg tacccatttt
     3181 tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag
     3241 atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt
     3301 tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg
     3361 ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct
     3421 gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga
     3481 tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata
     3541 ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga
     3601 aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga ttaaaagata
     3661 gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag gatgagcggc
     3721 cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg ctgaccttca
     3781 tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg tctactgaaa
     3841 ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta atattcctct
     3901 tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt gatcgttaat
     3961 ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa atattcttta
     4021 ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt cttttatgta
     4081 acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa ctaattgtac
     4141 ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt tttctttatt
     4201 gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa ttccatgggc
     4261 ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca gtcattttat
     4321 tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg gacatgaact
     4381 gagcacctac gatacgattc gttccttttt catagactag ctccatcatt actttttcag
     4441 tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg acttctagat
     4501 cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt
     4561 aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc aagttatttc
     4621 ccacaagcaa accttgacga acggcatttg ttgccaaagg aatatagttt tgcgtgccac
     4681 ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga ttacttgtct
     4741 gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt ccttcgatca
     4801 gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca aatgtatctg
     4861 ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca gtaacatttt
     4921 cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata tctgtgaatg
     4981 gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc acttgttttc
     5041 cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca acaattacta
     5101 cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag tttttgcata
     5161 ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat gaaccagttg
     5221 tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg
     5281 ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta actgttgctc
     5341 ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc tttactactc
     5401 cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt ctttcatata
     5461 ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca gtacctgcat
     5521 gagtacagcc aattactact actttcattt atggtccctc cgattaacat tttgtccttc
     5581 gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg tgaattcaca
     5641 aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct acaagattct
     5701 tattttaata atattcactt ttatatctaa gattgtgaat aaaagacaaa aaaataaaaa
     5761 agctagcgtt ttttttacac agctagcttt ttttaggtat aacacttgta gaaactgttt
     5821 ttacatttgc gccttttttg ctgactcttt acaatccgga cagagtccat acaattccaa
     5881 ccgatgttca ttaatctcaa aatcagtcag tctgcttgca gccatttcta tatcttcaag
     5941 ccctgggtaa tgaaaatcaa caatcttacc gcagttttca caaattacat gataatgacg
     6001 ctttgaactg aaatcaaatc ggctagaagc atcgccgtac ttcatttctt gaacaaatcc
     6061 aatcgaagta aataagcgaa ggttattgta aacagtcgca acactcatat ttggaaaccg
     6121 atgttcaagt gctttataaa tctcatcagc cgttggatgg gaatgatgtt caatcaaata
     6181 ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta acttttcgat
     6241 aacctcttcg actaacttat tttccatgta aacaccccac gttgttattc tcgtcttatc
     6301 atactatttt tacattttaa aatccactta acatgaaaag aaaattcaat tttattgtat
     6361 tttaatgaaa aaagacgtaa aacagtcgcg ttcactattc tacgtctcta ttcaaataat
     6421 ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct
     6481 atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt
     6541 caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt
     6601 ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc
     6661 caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca
     6721 cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc
     6781 ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt
     6841 tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac
     6901 ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg
     6961 tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt
     7021 tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag
     7081 tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct
     7141 caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc
     7201 ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt
     7261 ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa
     7321 actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga
     7381 ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct
     7441 ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc
     7501 gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaat
//
LOCUS       DADOOS010000126         7408 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.126, whole genome shotgun sequence.
ACCESSION   DADOOS010000126 DADOOS010000000
VERSION     DADOOS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.126"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            229..702
                     /locus_tag="J8C37_002299"
     CDS             229..702
                     /locus_tag="J8C37_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAZ5305719.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            699..2831
                     /gene="feoB"
                     /locus_tag="J8C37_002300"
     CDS             699..2831
                     /gene="feoB"
                     /locus_tag="J8C37_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAZ5305720.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2833..2967
                     /locus_tag="J8C37_002301"
     CDS             2833..2967
                     /locus_tag="J8C37_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAZ5305721.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3061..3705
                     /locus_tag="J8C37_002302"
     CDS             3061..3705
                     /locus_tag="J8C37_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305722.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3892..5085
                     /locus_tag="J8C37_002303"
     CDS             3892..5085
                     /locus_tag="J8C37_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ5305723.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5078..6805
                     /locus_tag="J8C37_002304"
     CDS             5078..6805
                     /locus_tag="J8C37_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ5305724.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7199..7396
                     /locus_tag="J8C37_002305"
     CDS             7199..7396
                     /locus_tag="J8C37_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAZ5305725.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         2413 a         1271 c         1639 g         2085 t
ORIGIN      
        1 tttaatagag aaaaaattgt ggaaaactat gaagaaatat ttttttctca tttttatcta
       61 tcaatactaa agattcgctc ataatatcac aagtataatt gacattctca attaccgaga
      121 gtattctatg tgagaatgat tctcatttat ttgcgtaaca cgtaagaaaa ttagaacata
      181 taatgaatga ttttaataca tgatttattt cacaagagag gaatcaatat gcagacctta
      241 gcgcaggcaa agcagaattt cgtttatcaa gtcgaacgaa tcgaaacaga agaaaaaaca
      301 aaacgccatt tgcaaaatat cgggatcgtt gccggaagta aaatcgtcgt cgtcaattta
      361 tcaggcaaca atgccatttt actggtaaaa agtcatcgag tggcagtaag tcgggaaata
      421 ctaggtcaaa tctttgtcaa taaaaagact gcttcctctg aaagctggac ttctcttgat
      481 caattaaaag caggagagac tgggatcgtc gtagcaatcc acggacaagg cgcagtgaag
      541 cgacggttaa tggatatggg attgacgcga aatgtaaaaa tagttatccg aaaactagcc
      601 cctctgggag atccaatcga actgacagtg cgagggtatg aactgacgtt acgaaagtca
      661 gaggccgaac taatcttgat acaaagggag gaaaaagaat gaatatcgcg ttagtcggaa
      721 atccaaacag cgggaaaact agcttgttca atctcttgac tggttcgaat caatatgtag
      781 ggaattggcc gggcgtaaca gtagaaaaaa aagaaggatc gttgaaaaaa aatccgaaca
      841 ttcatattca ggacctaccc ggcatctatt ctttgtctcc ttatacacca gaagaagtga
      901 ttgcaagaga ttatttggtt caggaacagc ctgaactgat catcaacatt attgatgcta
      961 ccaatttaga acgaaatctt tatttaacta cgcagttatt ggatttagac attcctatga
     1021 tcattggtct gaatatgatg gatgtcatca aaaaagaagg aaaaaaaatc aatctagaaa
     1081 agctttccta tggtcttggt gtaccagttt taccaatcag cgtattgaaa aagcaaggaa
     1141 tcgaaaaact aatcgaaaag attcaggagt ctgcaaaaaa gcggccttct attcctgttc
     1201 cctgtacata tgatccacgt ttagaagcag ctttacatga aattgaagat gtactaggag
     1261 aacaaagcgg caagaaacgc tggtatgcaa tgaaatattt tgaacgagat cagaaggtaa
     1321 gagaggatta cgagatagca gtttctcagc aaaaagagat cgagcaactg atccagttaa
     1381 cagagaaact attggacgac gatagtgaga cgattctggt caatgaacgt tatgaattta
     1441 ttacccaact atgtacactc tgtgttgtgt caaatgattc tttccaatta tcgatgagtg
     1501 acaaaatcga tcagattgca acgaatcgtt ggttagcctt gccgatcttc gcatttgtga
     1561 tgtggctgat ctattatctg gctattcaaa cagtaggaac aatggggaca gattggatca
     1621 atgacacatt gtttggtaca tggttaccag aacatgttgg cagattgtta gctgaatggc
     1681 aagtagcggg atggatgcag gagctgatct tgaacggcat catcgcaggc gtgggagcag
     1741 tgttaggatt tctaccgcaa ttgattatat tgtttttatg cttgagcttt ctggaagact
     1801 gcggatatat ggcgaggatc gcttttgtca tggatcgact tttcaggaaa ttcggtcttt
     1861 caggaaaatc cttcattcct atgctgattg ctacaggatg tggagtgcca ggcgtcatgg
     1921 ccagtcgcac gattgaaaac gagcaagatc gtcgtttgac gatcatggtc acaaccttca
     1981 tgccttgttc tgcaaagttg ccgattattg ccttagtagc aggggccttt tttcctcatc
     2041 aatcttgggt agctccttct gcctattttc ttggtatgac tgcaattatt ttctctggaa
     2101 tcagcttgaa aaaaacacgt ttgtttgctg gagatacagc tccttttatc atggaattgc
     2161 ccgcatatca ttttcctcaa tggtcagcgg tcctaagaca aacctatgac cgcagcaagt
     2221 cttttgttaa aaaagcagga acgattatct ttgtttctag tatcgtgatc tggttcagct
     2281 cttcttataa tttttatgct caacctgttt ctgaagacca aagtatctta gcctttttcg
     2341 ggcgaatcct ggcagttgtt tttcagccgc ttggttgggg aaactggcaa ggaacagtcg
     2401 cagcgattac aggattggtc gctaaagaaa atattattgg cacatttggt attttgtttg
     2461 gtcatgcgga agtatccgaa aatggcagag aaatctggca agtgctacaa cacacatata
     2521 ctcccgcagc agcttattca ttgttagcct tcaatttact ttgtgcgcct tgttttgcgg
     2581 caattggggc tatccatcgg gaaatgaatg ccaagaaatg gacttggatc gccataggct
     2641 atcaatgcgg tttagcttat ttagttagtt ttgtgattta tcagttggga cacttattgg
     2701 agggtggaca ggtagcgtta gggacgtatc ttgctatttt actgatgata ggactcgttt
     2761 atgttttgtt ccgtcagcca aaatccaaga accagtttta tactatattg tcattagaag
     2821 gagaggaata ggatggcgac attcttatta agtgtgttaa tctttggaag tgcaggcgca
     2881 attgtctata ctcgtttaaa atccggtaag aattgtgatg actgtcaaac agcttgccca
     2941 gtaaaaaagg aacagatcaa gcgatgatca gtcgttgcaa gaggctattc tccccttttt
     3001 tgcttatttt gctagagcat agtaagatga gggaaagtaa aaaaagaaag aggaaagaaa
     3061 atgtctggaa agaaaaaaat cgtccgcccg cgctaccagc agattgctgt tgatttagcc
     3121 gaacgtatcg ttgaaaaccg atataaagtg ggtgagaaga tccatgcccg ctcaacactt
     3181 gccagtacgt acaatgcttc tcctgagact acccgcaagg ctatcaatgt gctggtagat
     3241 cttggaatca tggaagtacg gcatggaagt ggggcatttg ttgcttcgaa ggaaaaagca
     3301 aaagatttcc tattgcaata tcaagatgta caatccatcc aagatacaaa agcagaaatc
     3361 atggcaagtg tggagaagca acaagatgag ctgaatcatt tttctcaatt attggatcag
     3421 ctggtcaatc aaacgaagcg aatacataag atgaatcctt tacttccgtt tgaattatct
     3481 ttatcagaaa aagcaatgca tctggaccgt tcaattagcg agttgaatat ctggcaggcg
     3541 actggcgcca cagttatcgc aatttctcat aatgaagaac tgcttgtttc acctggcccc
     3601 tatgctaaat ttacggcagg ggacatcgtt cattttattg gaaatgaata tacactacag
     3661 cgtatgacaa acttttttta cccggagcat cagcttgcag aatgattgtg tgaaatatta
     3721 aaaaaatatt aacttggcta aaccttttat ttacaaagat ttagccaagt ttttttcagt
     3781 ttggacaagc aaatttcttt catttttgac aaagtgacta catgatgtta acatagtgtg
     3841 gtcacttttt tcagaaagaa aaatgaattt acataaaaaa ggagaatttg tttggtaaaa
     3901 gtagaagtaa aacatttaac aaagatattt ggtaaaaaga cacaagccgc tttagatatg
     3961 atgaatgatc accagccgaa aacggagatt ttaaagaaaa ccggtgcaac ggttggtgtg
     4021 tatgacgtga attttgacgt aaaagaagga gaaatcttcg tgatcatggg gctttccggg
     4081 agtgggaaat ccacattgat ccgtctattg aatcgtctga ttgagccaac ttcgggcagt
     4141 atctatattg atggagaaga tgtatcgaag ttaagcaaag aagaattgcg agaagtccgt
     4201 cgtcataaaa tcaatatggt tttccaaaac ttcggtctgt ttccacatcg aacgatcttg
     4261 gaaaatacag aatacgggtt agaagtccgt ggtgtaccta aagaagaacg acaagaaaaa
     4321 gcagaaaaag cattagaaaa ttctagtttg ctttcattta aagaccaata tcctagtcag
     4381 ttatctggtg ggatgcaaca acgggttggc ctagccagag ctttagcaaa tgatcctgag
     4441 atcttgctga tggacgaagc gttctcggct cttgatccgc tgatccggcg agaaatgcaa
     4501 gacgaattac tggacctgca agccaatgtt caaaaaacga ttattttcat tacccatgat
     4561 ttgaatgaag ctttacggat tggggacaga atcgccttga tgaaagacgg cgaaatcatg
     4621 cagatcggaa caggtgaaga aatattgacc aatccagcaa atgattatgt acgtgaattt
     4681 gtggaagaag tcgatcgttc caaagtgctt acggctcaaa acatcatggt acctgcattg
     4741 acgacgaata tcgaatctga tggaccaaac gttgcattga caaggatgcg taatgaagaa
     4801 gtcagcatgc tgatggctgt tgacagaaag agacacctga aagggatcat cactgcagat
     4861 caagcgttag aagctaggaa gcaaaaacgt ccattgatcg acttcctaga tgaaaatgtg
     4921 acggtgattg ggaaagacat ggtggtaagt gatattttca atattattta tgactcaccg
     4981 actccacttg cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg aggtagtgtg
     5041 attgaagcat tagcagaaac aagcgaggtg agtgaacatg aataatctat tgaactatca
     5101 attaccagtg gcaaactggg tagaaaatat cacagaatgg tttacgacaa ctttttctgg
     5161 cctattttca tttttacaaa cgattggaca ggcagtgatg tcggggatta caaatttact
     5221 gttagtgatt ccagctcctt tgtttatctt gttattgacg gtagcagctt tctttatttc
     5281 taagaaacgt ccaggactta cgttgttcac attaatcggt ctatggttta tttataacca
     5341 aggtctatgg aatgatttga tgaatactgt cacacttgtt cttttatcta gtgtgatctc
     5401 gatcatcatt ggggtaccat tagggatctt gatggcaaaa agcagtaaag cccaaagtat
     5461 tatcaaaccg atccttgact ttatgcagac aatgccagga ttcgtttatt tgattccagc
     5521 agttgcattt ttcggtatcg ggatggttcc aggggtattt gcctcagtca tctttgctct
     5581 gccaccaacc gttcgtttta cgaatttagg gattcgacaa gttcctaaag aattagtaga
     5641 agcttctgat tcattcggga gcacaagtcg tcaaaaacta ttcaaactag aattgccttt
     5701 agctaaaagt acgatcatgg cagggatcaa tcaaacaaca atgctgtcac tttcaatggt
     5761 cgttattgcc tctatgatcg gtgcacccgg cttaggacgc ggcgtattat cagcattgca
     5821 aagagcacaa gtcgggaatg gatttgtcaa cggggttgca ttagttatct tagcaatcat
     5881 cattgaccgt tttacacagc atttgaacca gccaaacaat aaaaaagctg caggagcaac
     5941 aacacagaaa agtaaaaaac gtcaaggact gatcattggt gctgtagtag tagtgatctt
     6001 aggtgcgatt ggtataggga gtttttcatc tgccaaagaa acaaaacgta tcaacttatc
     6061 ttatgttgaa tgggatacag aagttgcttc aacaaatgta gtcggtgaag tattgaaaca
     6121 aatgggctat gatgtaacga tgacgccatt agataattca atcatgtgga aatctgtttc
     6181 aaatggcgag tcagatgcca tggtttcagc ttggctacct aaaactcatg gttcacaata
     6241 tgcacaatat aaagatcaag tcgaagacct aggtgccaat ttgacaggag caaaagttgg
     6301 tttagcagtg ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg atcaggcagg
     6361 taagaaaatc ataggaatcg agccaggagc tggtgtagtt acggctgctg aaaacacgat
     6421 ccaaaaatat gacaacttga aagactggaa agtagagaca tcttcttcag gagcaatgac
     6481 cgttgcacta ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag gatggacacc
     6541 tcattggatg tttgccaaat atgatctgaa atacttagaa gatcctgaaa acggaatggg
     6601 aagcgaagag cagatccata cgatggttcg aaaaggattg aaagaagacc agccagaagc
     6661 ttacaaagta ttagacaatt tccattggtc agaaaaagat atggaaaaag ttatgttgga
     6721 aatcaacaat ggaaaagatc cacagcaagc agcaaaagac tggatcaaag agaaccaaga
     6781 attagtcgaa agctggaaaa aataaaaaga cagacacgaa ataaatggag cttccaaaat
     6841 tggatcaaat atccaaattt ggaagctcca tgttcgtctc tatctttttt gtctataaaa
     6901 aaagagaatg cgtagctcat cttcgaaacg tccaaagacg ccgacaaact attatgaatt
     6961 tatgggtgcg agtcaaagag atcgatcgta ggtatgaaag actaatgtga ttaaagttta
     7021 ttatgtacga agtgcattct caaaaaatat taaagaatat acatagttta tatacttaaa
     7081 agtgattaaa ttcttattca ggaattattt tgctacgttt caagaaggaa ttgttttttg
     7141 aaatttaatg aatgttagaa taatagtcta aaaacattaa tttaggggtg attagattat
     7201 gaaacattta aaaattttgt ctattaaaga gacacaactt atctatgggg gtaccactca
     7261 tagtggaaaa tattatggaa atggagtgta ttgcactaaa aataaatgta cggtcgattg
     7321 ggccaaggca actacttgta ttgcaggaat gtctataggt ggttttttag gtggagcaat
     7381 tccagggaag tgctaaaatg aaaaaaaa
//
LOCUS       DADOOS010000127         7408 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.127, whole genome shotgun sequence.
ACCESSION   DADOOS010000127 DADOOS010000000
VERSION     DADOOS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.127"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(13..348)
                     /locus_tag="J8C37_002306"
     CDS             complement(13..348)
                     /locus_tag="J8C37_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAZ5305726.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..604)
                     /locus_tag="J8C37_002307"
     CDS             complement(332..604)
                     /locus_tag="J8C37_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAZ5305727.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="J8C37_002308"
     CDS             complement(1055..1858)
                     /locus_tag="J8C37_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAZ5305728.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="J8C37_002309"
     CDS             complement(1836..2960)
                     /locus_tag="J8C37_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305729.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="J8C37_002310"
     CDS             complement(2976..3791)
                     /locus_tag="J8C37_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5305730.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4662)
                     /locus_tag="J8C37_002311"
     CDS             complement(3781..4662)
                     /locus_tag="J8C37_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAZ5305731.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="J8C37_002312"
     CDS             complement(4738..6027)
                     /locus_tag="J8C37_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAZ5305732.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6255..6899)
                     /locus_tag="J8C37_002313"
                     /pseudo
     CDS             complement(6255..6899)
                     /locus_tag="J8C37_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1589 c         1136 g         2405 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DADOOS010000128         7334 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.128, whole genome shotgun sequence.
ACCESSION   DADOOS010000128 DADOOS010000000
VERSION     DADOOS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.128"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1071)
                     /locus_tag="J8C37_002314"
     CDS             complement(<1..1071)
                     /locus_tag="J8C37_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAZ5305733.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESK"
     gene            complement(1071..2207)
                     /locus_tag="J8C37_002315"
     CDS             complement(1071..2207)
                     /locus_tag="J8C37_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAZ5305734.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2239..3690)
                     /locus_tag="J8C37_002316"
     CDS             complement(2239..3690)
                     /locus_tag="J8C37_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAZ5305735.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3734..4498)
                     /locus_tag="J8C37_002317"
     CDS             complement(3734..4498)
                     /locus_tag="J8C37_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAZ5305736.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4526..5224)
                     /locus_tag="J8C37_002318"
     CDS             complement(4526..5224)
                     /locus_tag="J8C37_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAZ5305737.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5236..6015)
                     /locus_tag="J8C37_002319"
     CDS             complement(5236..6015)
                     /locus_tag="J8C37_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAZ5305738.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6031..6975)
                     /locus_tag="J8C37_002320"
     CDS             complement(6031..6975)
                     /locus_tag="J8C37_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5305739.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7021..>7334)
                     /locus_tag="J8C37_002321"
     CDS             complement(7021..>7334)
                     /locus_tag="J8C37_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305740.1"
                     /translation="EKVTSADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQL
                     DQLFDTMLKDGQVTATATYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNIS
                     Y"
BASE COUNT         2054 a         1576 c         1107 g         2597 t
ORIGIN      
        1 ttttgattct aagattttgt atgctgtttg aatattataa tctgccttta ttaaaatcga
       61 acgttctgaa tctcgatgag agatatctac atttgcaatc tctctcgccc attcttttgc
      121 cgattgattt aaacttatga aattggtctt atcactgaaa gcaacttctg taggaacttt
      181 ctccgaaaag aaacacggta aatctgcaaa ttgagcttca atacccacta acggaacccc
      241 ttcaaataat gatggcaaca ttaatacgtc aaatgcttga taaaattttt ctatatcatt
      301 tctatttcct aaaaatttaa tatcatcatc tatgccactt ttctttgcat aattttttat
      361 ttcttcttcc atatcaccag ttccaataat taataatacg gaattattct taatagcgtg
      421 aaattcttta aaaatttcaa tcaaaaaacg atgattttta gaatatgaaa ttcttccaat
      481 atgtcccatt acaaacgtct gacttgaaat tcctaatttc attcgtacat catttctaat
      541 actttcatca taataaaatt ttttagcatc cactgcattt ggaatcaaag tgaatttatc
      601 atttcgaaac aaaaaatcac ctgctacttt tccacatgaa aattcatgtg tcaaaacaga
      661 cgttaactta catcgataat attgctttaa aagatattta aaatctctat ctatcgatgt
      721 attatggcta tgtgctatcc gtatcggaat cccagctttt tttgcagctt ttaaaggtaa
      781 ataactcatc gcatcaatat gagaatgaac aatctcatat tctggatgat ccttaaaaaa
      841 ttgttccatc catttaaaat atttaggata attttgtgga taaagtctcg gtgcataata
      901 gacctttcct ccaagactta ttatttcctc atcataatca cttttatatg gacgatgtgt
      961 caaaaaatca aactgaattt tagttcgatc tatattccta taatagttca ttagcatcgt
     1021 ctccaaccct gcacgatgca tatcattcac acactgtaat attcgaatca ttatatattt
     1081 cccctataca aatttaatgt ttcttgtacg attttatccc aattatattt actacaaata
     1141 aactcagtag cttgattctt caaaacttta accatttccg attgattgca tgcctcttct
     1201 aatcttatct ttaaatcact tacatccgat ttcttaaaaa tcatagcctt gtcttctact
     1261 acttccgtac actctgagat atttgaaact atacaacagt tcccataact cattgcttct
     1321 aacaaactta atggcattcc ttctaaatca cttggaagtg tatagatata cgcattacta
     1381 tataattctt ctaattcctg tccttgtaca aatccagtaa atataattcg ctcatcacct
     1441 tttgctaatt cctttagttc atttgcaaat tcatctgtat cagagcttcc tccggctatt
     1501 ataagttttt tatctgtctg aacatcttta aatgcctcaa ttagataccg aattcctttt
     1561 tcgggaacca atcgccccaa aaacaaaata taagaatctt ttgttaatcc atacttttct
     1621 gtaatcaatc ctgctgattt tatttcaatt tttttaacgc catttggtat aaaccttgtt
     1681 tttcttccat atatatcttc aaaataattt tggacgcttt cactaagtac aattatttca
     1741 tctgcaaatt ttacagcaca cttttcccct aacatgatgt atgtactagc aagcttgttc
     1801 catttttctc tttgatgatc tagaccatga attgttgcaa cacatctttt cccaaaaagt
     1861 tttggcaacc acaacatagc acatggtcct tctgcatgaa aatgaacaac atcatatttt
     1921 ccgaatgctg ctctaattcc accaaatact gaagaactca ttgctgcaat tccttttata
     1981 tttaatgtaa aaatagtctt taattttaca cctttatatt ctttattttt atgctggtca
     2041 aattcctttc cgctaacatg atgaccactt cgattatagc aagtcacttc atgcccaagt
     2101 gcaaccattc gcgttgccaa ttcttctacc actatctcaa ttccaccttc tcttgatgga
     2161 attcttttat gacctagcat acagattttt aatttttttc ttgccatcac gttttactcc
     2221 ttctatagta tctttatctt acatggaacc gtttctatga agcacagttt tgactgtcct
     2281 aaacaaaatt ctaaaatcta atcccatact ccaattcctg atatattctg catcaagttt
     2341 tacaacctct tcgaagtctg tgatatcact tctaccactc acctgccaca ttccagtaat
     2401 cccaggtttc atcgcaattc tcgcacgatg atgaaaatca tacttctcat attctgactt
     2461 caagcaaggt ctaaacccca ccaggctcat ttttcccatt aggacattat aaaattgtgg
     2521 aaactcatcc aatgatgtag atcgaataaa ttgtccaatt cctgtcttct ttgttccatc
     2581 aggaagtatt ttattgccaa tgattcttgg gtcaaaatcc atcttaaaca tgagattatt
     2641 actcatctta ttttctttca tcaactctgc tttgcgttct tctgcatcca tatacatact
     2701 acggaactta tacatcttaa atgtcttacc attttgacca atacgttctt gtgcaaagaa
     2761 aatcggccct ggtgatttga tgtaaataac tggtgctacg aagataaaaa taacacctgt
     2821 tgcaatacat ccaataattc cagcaagaat gtcaatcgtt ctctttataa atgcttgcct
     2881 catcgtcata acattaatac tagatgtaat aacagtataa tcaccaactc ggccaataaa
     2941 ttgttttcca cgtgtactat cagacacttt tgtaagattc atatgtacag caagtcccat
     3001 ttccgaacat ctggcgatta attctttggg atatggaaca ctttcgtcaa cattgatgaa
     3061 tacttcatct acccaattct gtaaaagata ttccgctgca ttatcagcat ttgcaactac
     3121 tgggacacct ccgatggtag ttccatacat atctctgtct actacaatca caccactaag
     3181 tttatacatg ccataattct tctctttaag attacgaact acattttttg caatcttttg
     3241 agatgttaca ataactaacg atacatcttc accgtttttc atgcggtgat gcaacaaccc
     3301 tttccaaagt atacgaatta tatatgttaa taatccatac accaccccca ttataaataa
     3361 agaaactctg gaataatcat ttccatcctg cattgtaata aggtataaac tgcacacaag
     3421 taacagcata aaagattgct ttatagaagc tacagcctct ttataatatc ctctttttaa
     3481 aacattttta tatgcctcaa ataaaaatat aatcgtaaga ttctcaagtt ctataaaaat
     3541 tgccatgtgg cgataaatag gaatcacata cggattccag tcaccctgtc gcattacata
     3601 tgatatataa aaagcaagct ggacacaaaa taagtctaat aaaataaaat caatatgttt
     3661 agaccaacca cttgagtttt ttctatacat ctctcactct ctcctcattt cctttttatc
     3721 tacgaataaa gaactaaaat aaaccaaact tcttcttttt tacctcttca tagattggat
     3781 aattgatctc atccccgttg atcagatcct ttgcgacttg tttcatctgt actacttttt
     3841 ccttaccaaa ctccttggca atctgctcat agcactcttt taagtaaaag gtgcgcttag
     3901 agaccccgtg tgcatcagaa gcagccatct gcacgagatt atgtttgacc attgttttgg
     3961 ctgttttctg gatatctttg ccaaatacac caacataact tggcgcagta agttgggcca
     4021 aacatcccat atctaggaac ggaagcaaat gatttgggtc ttctcgaaaa tgcgcgttgc
     4081 gttctggatg gacgatcact ggtatctttc ctagttctaa caactcaaaa aaaagccgtt
     4141 ctgcatataa cggcacttcc agtgtcggaa attcgatcag aagatatgta tcatctaaat
     4201 ctgtaaataa aatttcatct cggtggatgt cttcgatgag cgttccggta atacgtactt
     4261 cttgtccttc caataacgtc aaaggcaact gtcttttctc taattcggct tgtaacgaag
     4321 cgactagaga aatgacttgt gatttttcat tgctgtattt tccattattg tggtgtggtg
     4381 tgcataaaat atgcgtaatg ccttgctgaa tcgctttttc agccattgca atacttgctt
     4441 ctaagttttc tgccccgtca tcgattcctg gtaggatatg gcaatgtaga tcgatcattt
     4501 ctcttgtctc ctctcttctt attccttaat ttccgtagta ataaccagca tctttgctgt
     4561 gttctgctcc gttgtatacg acacctaaga ctcgtgcttg taccatatct aacagttctt
     4621 ttgctttagt caatgattct tttcgtgacg tattttcccg tacgaccaat aaggtgccat
     4681 ccgtttttga agacatgatc tgtgcatctg ttacggcaac gactggcggc atgtcgaaga
     4741 tcactacatc aaatagttgt cttgcttctg ctaaaatctg atccattctt ggcgaactta
     4801 gtagttctga tggatttggt ggtttaggtc cacttggcag aatcgacaaa ttgtcgactg
     4861 gtgttcgttg gatctcatct gctacacttc ctgatgaact aagaacagta cttaatccgc
     4921 ttgcgttgtt caacttgaac gttttataga tgacgggctt acgtaagtct gcatcgacta
     4981 gcagcacccg ttgaccagat ttggcaaaaa caacggcaat gtttgctgca gtggtcgatt
     5041 tcccttctcc aggtcctgat gaagtcacaa caatcgtttt gatttgctga ccagctgcgg
     5101 aggcaaactg gatattcgtc cgaatcgtac gatattgttc agcgattggt gaagagggat
     5161 tggtgagtgt gaccaaactg actgcatgag tttgggctgc tttttgtttc tgtgttcgtg
     5221 ccattttctt tcctcctaga ctcttgagcg gcttctgcgt gatggcgctg ttttatcgtt
     5281 tgtagctttt ggttgtgtct gagctggtcc ttttttacgg ataccagcat tcaattcttt
     5341 cgctgacatt tctggaactg ttcctaagat tgggaatcct aatgtatctg tcacatattt
     5401 ttcatctttc actgtccgat cgagtaattc caataagaat gccagaccta cacctaccat
     5461 cattcctaac attaagccag tcattaaatt caatttatta tttggcgaaa caggtgtcgt
     5521 attggcaact gcacctgaaa tgatcgagat tttatcaaca ttcatcacat cttttgcgtt
     5581 ctcttggaaa acttgtgaag ttgtgttagc gatctgttga gcagccactg cattcgtact
     5641 tattgcctga atcgagaaca tttgtgaatt ttctgcctga ttaactgaga tggcatcttt
     5701 gatttgttca gcagtcattt tcacattgtc ttctgtttct aaacgatctt tgacttcatt
     5761 gagcactaaa tcactgacga tcatatcttt atacgtattg atcatttgca ggttcgtatt
     5821 gacatcattg acatttgcag tgtctgattg tggcagtgtc acgatcaatt gtgcttgtga
     5881 actgtatttt ggtgtcaaga tgaaaaatgt tacgattcct gagatggcca agccagcaaa
     5941 cattgagatg atgattatta ccaagtgttt gcgtaatgtt tcaaaaattt ctttcaagct
     6001 gattgtctct tccatttttt ctccactttc ctatttattc tctagattca tttgatcttt
     6061 taattctttt ttcacacgat caagttcttg atcactgact ttttgatagg agatattatc
     6121 ttgcatgaag ccttctcctt gcagctgatc ttgcttaatc gtaccaaatg cctcccgata
     6181 atctaaagca atttttttca tttcatcaaa gttcatgtct gttttcatat tcgactctag
     6241 agcattcaaa acggattgat agttgctgac accgtttaga ctcaatactt ttttcgctac
     6301 gccttctacg atctttcttt ggcgcccttg tctaccgtaa tcaccatttg ggtcttcata
     6361 acgcatacga gaataagata atgcctgatc cccgttcaat gtaattttac cgattggaaa
     6421 gtcatagcca tcttgtgaga aagtgaggtc attattgact tcgataccac caactgcatc
     6481 aacgagttcc tttagccctt tcatattgat agagacatag tgatcgattg ggatgtctac
     6541 atatttttgt acggtatcca tcgccatggc agctccgccg aatgcgtaag cgtggttgat
     6601 cttgtctttt gtgtcacgac ctacgatgtc gacatacgta tcacgaggaa tgctgacgat
     6661 cgttgtctgt ttgtcttcag gactgactgt ggcaaccatc atcgtatctg atcttccttg
     6721 atcgacccga cctaaatctc cagtatcaat ccccatcaat agaacagaga aagattcttg
     6781 tttgtccaga tcaactggtt tcgttcgttc tccttgggtt cgttccactg attcgtaagt
     6841 tttctcaata gaatgtgaga gatccagata taattttgct cctactccta caattgctaa
     6901 tactaatacg ataagaactg ctaaaataat cagaaatatt tttttccctc ttgattttct
     6961 tcttcgtctt gccatagttt cctccgtttg ctaaccttgc ttgaatcaac gttttaactg
     7021 tcaatagctt atattgctat agcccatttg ctcagcaaca gtttgtgctt ttgttgcgta
     7081 tgctgcgctt attttttcat tccggatctg ctctatttga ctgagcaccg tcaaatattg
     7141 atcgtaagta gcggtggcag tcacttgacc atcctttaac atcgtatcga ataactgatc
     7201 tagttgcgtg acttgcgcga tctgaatatc agcgaatcct agatattgca taacaaccgt
     7261 tttccaagga ctatctttaa aaaagctcat attttcacgt acacgagcga catctgcact
     7321 tgtcaccttt tctt
//
LOCUS       DADOOS010000129         7056 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.129, whole genome shotgun sequence.
ACCESSION   DADOOS010000129 DADOOS010000000
VERSION     DADOOS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.129"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(74..1420)
                     /gene="gor"
                     /locus_tag="J8C37_002322"
     CDS             complement(74..1420)
                     /gene="gor"
                     /locus_tag="J8C37_002322"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAZ5305741.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1554..2795)
                     /locus_tag="J8C37_002323"
     CDS             complement(1554..2795)
                     /locus_tag="J8C37_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAZ5305742.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(2813..3736)
                     /locus_tag="J8C37_002324"
     CDS             complement(2813..3736)
                     /locus_tag="J8C37_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAZ5305743.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(3957..6449)
                     /locus_tag="J8C37_002325"
     CDS             complement(3957..6449)
                     /locus_tag="J8C37_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAZ5305744.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6456..6923)
                     /locus_tag="J8C37_002326"
     CDS             complement(6456..6923)
                     /locus_tag="J8C37_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAZ5305745.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         1923 a         1550 c         1145 g         2438 t
ORIGIN      
        1 aaaatcagta tactgatttt ctggttttac atggtttgtt caataaaaag ctgttggaac
       61 tgttaatatt aaattatcgc atcgttacga attcttccgc tcctgttgga tggatggcta
      121 ctgtattgtc aaagtcttct ttagttgcac ccatcttgat agccacggca aaaccttgca
      181 gcatctcatc gacaccaaca ccaatagcat gtaagccgac gattctttct tcttttccga
      241 cacagatcaa tttcatttcg catttttggc gataatcatt taatgcaaag tacattgggg
      301 taaagcgtga acgataaact ttcaattcat tttcgccata tttttccaat gcttcttctt
      361 ctgtcaaacc aattgttgca attggtggat gcgtaaagac gactgtcgga acaagtttat
      421 agtcaagata agaatctttc tttccattaa ataaacgttc tgataaacgt ctaccagctg
      481 cgattgcaac aggagtcaga tctaatttac cgatcacatc accaacagca aaaatatgtt
      541 catccgttgt attttggtat ttatctactt tgataaaccc ttttttgtcc aattccacat
      601 ttgtattttc cagacctaat ttttcggtat tcggtacacg tccaccggca aaaagtacac
      661 aatcaccagt taaggtttca ccattttcaa atagcaccgt atattcgtca ttgtttttag
      721 taatttcttt agcaatatga tgtgcgtaag tttttattcc agcttcttgg tacatttgga
      781 tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg tcgttctttt cgatatgccc
      841 acatgacttc actacctaaa ccacgaaata cacctgaaag ttcagccgca atgtatccag
      901 cacctaaaac gattgctctc ttaggtaact ctgttaattc aaaaaatcca tttgaatcaa
      961 ttgcgtattc tccaccagga atggtcattt tacttgcacg gcctccagta gcgatcaaga
     1021 ttttaggagc acgataggtt tcaccgttta cttcgatcgt ttgactatcg ataaaagtag
     1081 cgtaaccttt tatagcctcg acatgattgc tatctaatcc tctttgataa gcaccgtgta
     1141 gaaaatcaat atatttttct cgtctttcta ctagaccggc gaaatcaaca ttttttaaat
     1201 ctgcttggat accgtaactt gaggcatctc gttcaattgt ttccaaaatc gtacttgctt
     1261 gccacataac ttttttaggt acacaaccga cgttgacaca tgttccgccc aattcattcg
     1321 cttcaatcag caaaactttt gcgccatgca tccctgctct gtttgctgaa gcaataccgc
     1381 cagagccgcc accaattacg atgtaatcat atatttgcat tttgttcctc cttattgaag
     1441 agatcaatca aaacctttat ttaacgatcc tttttctaca gaaaacaagt gtaacacttt
     1501 tcttgaattt tgtatgcgaa gatgcttaaa acagggatat atcagctttt tccttattta
     1561 cgttttcgga tcatatctcc aggtttcacc ggctgtgcga ctggcatagt taaaatcatc
     1621 attggatttg gtgctcggtc aatggattcg ccttcttcat tatgcataac ttcaacggtt
     1681 tgactgaaat gactgaatcc tggaccataa aattcgattt catctcctac agaaaacaag
     1741 tttctctgac gtatcgtagc aatctgtgtt gcctcatcat aagataatac ttcaccgaca
     1801 aatttatata ctgggatttt acgacgttca ccgaataatt gttcgttttc agttggtgta
     1861 ttgtagtaaa aacctgtagc taattctcgt tgtgctactt tccataactc gtcgatccat
     1921 tcttgcttgc agacatagtt ttcaggatct tccatataac tgtctacagc cgctttatag
     1981 acatttgaca ccgtagatac ataatggatc gatttcattc ttccttcgat cttgaagctg
     2041 tctacgccgt tttcaatcaa ttctggaata tgttcgatca ttgacatatc cactgcactc
     2101 atcgagaatt cttcttccac ttcgcctgta tcagttaaac tgtttcgttc ttgaccaaaa
     2161 ggcatatcga acaatccata cttccagcga caagattgag aacatccgcc acggtttgca
     2221 tcccgcatcg acatatggtt ggacaatgtg catcgaccag aataagagat acacatggca
     2281 ccatggataa atgcttcgat ttcggtatcc gtatttttac gaatctctgc cacttcctcc
     2341 attgaaactt cacgagctaa aacgacacgt tccaaccctt cttctttcca gaattccaat
     2401 gtctcaaaat tagttgctga agcttgagtg gataaatgga tcggcagacc tggggcttct
     2461 gtcgcacaaa tttcgatcaa agctggatca gaaacaatca ctgcagaaat accgacatca
     2521 cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc cttcatgggt gaccatatta
     2581 gcagcaacgt ataccttcgc atcatgttct tttgcaaaag caactccttc agccatttct
     2641 tcataagtaa aattacctgc acggcttctt aaaccatatg cattcccgcc aatatatact
     2701 gcatctgctc cataatggat cgcggttttt aacttttcca gtgttcctgc tggcgctagg
     2761 atttctggtc gtttcaattt tctttttact gtcatttctt tcatcatact ccttatttga
     2821 tagcagaagg atcaatataa aagaatcctg tatctaattc acggttttca ggatgcaatt
     2881 tactgatttc gctagtcatt ttttccgctt tttcttgatc ccagctacct tctaaaattt
     2941 gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa tttttcgcct ggagtaaata
     3001 atccgtccaa tttccatgta cagtaatgat gctgataaag ttcattcaat tcattcgaga
     3061 gattcagatc attgctggca aaaatatgtg tgccatgact atcttcataa atagaataat
     3121 gtgtctcttc ttttttaggt tccgagataa aaagacctct ttcacgatct ttttgttcgt
     3181 cttgtttcgt atagttatag taattctgta gcagtggacg tttggattga tggatgcatg
     3241 tagcaccata cacaagggtt tctacaggaa tatcgagttt ttcttccatc gccttcatct
     3301 cttcaaaagg aacttcacga gcaagtactg ctccaactgc gccttttttc gcccagaaat
     3361 tgatttgacg tgcgctcgtt actaatgttt cagcatcata aatatacggt atacgcaatt
     3421 caggattttt tttcatcgta taaataatac ctgtatcacc aactgtgatc acatctacgt
     3481 ttatttcttt taaaaaagcc aaatattcag gtacattttt cattttttca ggatgaatga
     3541 tcccatttac agcaatattc gcttttttac catacttgtg tgctaattct accaattgtc
     3601 gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc aaattgctct tcaccgaaat
     3661 atagtgtatc tgcacctgca gcaagcaact gttctgcttg ttcaatagtt tctactgtgg
     3721 ttatgagttc gatcataatt ttctcctcct acaacttcat aatagtagca tagctctaaa
     3781 aactttccaa tacttcttct atttaaagtc ggatttttga aaattatttc acaaaaaatc
     3841 aaaataaagt tcgatatatt ctgtccaaat ataaaaagag attgtgacaa aaatcttgtc
     3901 acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc aagtaactat
     3961 agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt accttttgaa
     4021 gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc ctcgctcaat
     4081 cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata ttcaggatca
     4141 aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg aatatcttgt
     4201 tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg gatttcatct
     4261 ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc aggtcggaaa
     4321 gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt agtaacatct
     4381 tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat atttgaagtc
     4441 ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt taaatgacca
     4501 tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc cacttcatct
     4561 aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc accttcatca
     4621 taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt ttccataaat
     4681 tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc tgaaagtgct
     4741 ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga accaattgga
     4801 cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac tgctttgaca
     4861 gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa caagcgttcg
     4921 ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac aactgtagca
     4981 acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc aattgcagaa
     5041 actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc agcattttca
     5101 aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat ttctagtttg
     5161 atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc tgctgattca
     5221 tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg aacagaaaga
     5281 ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc ttcatatctt
     5341 gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc tacttgaatg
     5401 cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata ttcatctagg
     5461 gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa gatattcgaa
     5521 gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc atcaatgaat
     5581 aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg atcttcaaat
     5641 tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat caggcgcttt
     5701 tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc ttcagcaatg
     5761 gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt ccgtcggcta
     5821 agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc cagcttgttt
     5881 tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt tccttgcggt
     5941 gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt tccagcagaa
     6001 tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag gctgatacct
     6061 aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct catcaaaata
     6121 tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc taaagcaaaa
     6181 acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga acggatacca
     6241 ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa atccatttca
     6301 cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa aacatgttct
     6361 gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc taatactgct
     6421 tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc ataacttaaa
     6481 cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc attgaaattc
     6541 aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt aattatttgg
     6601 tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt atcatcaaat
     6661 aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt tgcaattcgt
     6721 atataaccac caccaccacg tttgctttct actagataac cacgttggat ggtaaaacgg
     6781 gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc catttcaatc
     6841 cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga ttcaattaaa
     6901 tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg accttaatta
     6961 taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat cctttagaat
     7021 caacaaaaaa aaatacggaa tatactattc cgtatc
//
LOCUS       DADOOS010000130         6960 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.130, whole genome shotgun sequence.
ACCESSION   DADOOS010000130 DADOOS010000000
VERSION     DADOOS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.130"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="J8C37_002327"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="J8C37_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAZ5305746.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /locus_tag="J8C37_002328"
     CDS             complement(1552..2973)
                     /locus_tag="J8C37_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5305747.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="J8C37_002329"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="J8C37_002329"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAZ5305748.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="J8C37_002330"
     CDS             complement(5384..6640)
                     /locus_tag="J8C37_002330"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAZ5305749.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
BASE COUNT         2051 a         1483 c         1261 g         2165 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
//
LOCUS       DADOOS010000131         6924 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.131, whole genome shotgun sequence.
ACCESSION   DADOOS010000131 DADOOS010000000
VERSION     DADOOS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.131"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            94..1248
                     /locus_tag="J8C37_002331"
     CDS             94..1248
                     /locus_tag="J8C37_002331"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAZ5305750.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="J8C37_002332"
     CDS             complement(1270..2022)
                     /locus_tag="J8C37_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAZ5305751.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="J8C37_002333"
     CDS             complement(2386..5052)
                     /locus_tag="J8C37_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAZ5305752.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="J8C37_002334"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="J8C37_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAZ5305753.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2043 a         1421 c         1252 g         2208 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DADOOS010000132         6821 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.132, whole genome shotgun sequence.
ACCESSION   DADOOS010000132 DADOOS010000000
VERSION     DADOOS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.132"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            43..933
                     /locus_tag="J8C37_002335"
     CDS             43..933
                     /locus_tag="J8C37_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAZ5305754.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1006..1830)
                     /gene="nadE"
                     /locus_tag="J8C37_002336"
     CDS             complement(1006..1830)
                     /gene="nadE"
                     /locus_tag="J8C37_002336"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAZ5305755.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1842..3314)
                     /locus_tag="J8C37_002337"
     CDS             complement(1842..3314)
                     /locus_tag="J8C37_002337"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAZ5305756.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3951..4313)
                     /locus_tag="J8C37_002338"
     CDS             complement(3951..4313)
                     /locus_tag="J8C37_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAZ5305757.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4536..5288
                     /locus_tag="J8C37_002339"
     CDS             4536..5288
                     /locus_tag="J8C37_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ5305758.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5428..6351)
                     /locus_tag="J8C37_002340"
     CDS             complement(5428..6351)
                     /locus_tag="J8C37_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAZ5305759.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6366..6638)
                     /locus_tag="J8C37_002341"
     CDS             complement(6366..6638)
                     /locus_tag="J8C37_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305760.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2088 a         1353 c         1163 g         2217 t
ORIGIN      
        1 atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac
       61 gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag
      121 ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt
      181 gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa
      241 gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatattag ttttccagaa
      301 aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat
      361 tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat
      421 gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc
      481 ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg
      541 gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat
      601 atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct
      661 gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg
      721 ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac
      781 atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa
      841 gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat
      901 caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca
      961 aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc
     1021 atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat
     1081 tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata
     1141 tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt
     1201 cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg
     1261 tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa
     1321 gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc
     1381 agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc
     1441 gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat
     1501 atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc
     1561 atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt
     1621 ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg
     1681 gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt
     1741 cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa
     1801 ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt
     1861 tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta
     1921 gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa
     1981 tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt
     2041 tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta
     2101 ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca
     2161 tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac
     2221 acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc
     2281 atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc
     2341 tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc
     2401 atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc
     2461 gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca
     2521 ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc
     2581 gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta
     2641 tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa
     2701 tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt
     2761 gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt
     2821 gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct
     2881 gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat
     2941 tggcagtgcg ctaaaggccc ttccacttgg atcaatggtt catttgcaaa aactagttct
     3001 ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa
     3061 ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga
     3121 ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa
     3181 ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag
     3241 tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg
     3301 taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa
     3361 caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg
     3421 gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta
     3481 acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg
     3541 ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta
     3601 agataccgat gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag
     3661 tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata
     3721 acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg
     3781 aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct
     3841 gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttttattg
     3901 tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta ttaaatagaa
     3961 tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt gaattgtatt
     4021 ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc tcgtgtcatt
     4081 tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt cttcgtattg
     4141 atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt tcgatctaac
     4201 gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac tactttagga
     4261 taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt catctcattc
     4321 ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc tttcctagca
     4381 ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt tatcacttaa
     4441 tagtataaca taatttcatg agaaaaaaat acaatttttt cttgaccatc tcgttacgta
     4501 aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata aagaaaatgt
     4561 cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt ggactgctaa
     4621 aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa gtcgatcgtc
     4681 tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc caagaggtct
     4741 tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa atgttgctgg
     4801 aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt gatatgtacg
     4861 aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa caaaagttga
     4921 aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa aaaaaggtta
     4981 cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa atgcaaactg
     5041 ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa gatgaaagct
     5101 tagcagaccg tatcttccaa gctcataaga agtggttatc ttacagctgg cctgaatatc
     5161 agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg ttcactgcat
     5221 attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt atccaaaaaa
     5281 aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct aataacaaaa
     5341 cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca ttctttcaca
     5401 agttttttca tcctacttta aatatattca tcgttgaata aacgtataag gtattttcac
     5461 tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag cttccagatg
     5521 gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc gtccaatcac
     5581 cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca tatccgaatt
     5641 tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt ttcctttctc
     5701 tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa gttgatgaca
     5761 tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca acgtgatgcc
     5821 caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg attgttctcg
     5881 atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa ctagaggtcc
     5941 atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa tgatcaatcc
     6001 atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt tctgatagcc
     6061 cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga tcccactgat
     6121 caactgatca aaatacggat gattggtata aggcagaatc actccaatgc ttttcgtttt
     6181 tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt tcatgaccgc
     6241 aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta cacgtgaaat
     6301 agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca ctgctgcttc
     6361 gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc gcattcaaaa
     6421 aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca atatatatct
     6481 tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct attcaaccct
     6541 tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata aaaataattg
     6601 ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc ttttgatact
     6661 ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca aagaaaagtt
     6721 ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat tataatgata
     6781 ctgtaaatat atctttatta ttagtgaatg gatatttact t
//
LOCUS       DADOOS010000133         6706 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.134, whole genome shotgun sequence.
ACCESSION   DADOOS010000133 DADOOS010000000
VERSION     DADOOS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.134"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(7..1164)
                     /locus_tag="J8C37_002342"
     CDS             complement(7..1164)
                     /locus_tag="J8C37_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305761.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="J8C37_002343"
     CDS             complement(1167..1523)
                     /locus_tag="J8C37_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305762.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="J8C37_002344"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="J8C37_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAZ5305763.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="J8C37_002345"
     CDS             complement(2991..3830)
                     /locus_tag="J8C37_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAZ5305764.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="J8C37_002346"
     CDS             4015..4902
                     /locus_tag="J8C37_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAZ5305765.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="J8C37_002347"
     CDS             complement(5486..6181)
                     /locus_tag="J8C37_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAZ5305766.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="J8C37_002348"
     CDS             complement(6165..6563)
                     /locus_tag="J8C37_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAZ5305767.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2108 a         1319 c          987 g         2292 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DADOOS010000134         6692 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.135, whole genome shotgun sequence.
ACCESSION   DADOOS010000134 DADOOS010000000
VERSION     DADOOS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.135"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            99..746
                     /locus_tag="J8C37_002349"
     CDS             99..746
                     /locus_tag="J8C37_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAZ5305768.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="J8C37_002350"
     CDS             1139..3784
                     /locus_tag="J8C37_002350"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAZ5305769.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="J8C37_002351"
     CDS             4094..5407
                     /locus_tag="J8C37_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAZ5305770.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="J8C37_002352"
     CDS             5397..6050
                     /locus_tag="J8C37_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ5305771.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="J8C37_002353"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="J8C37_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         2203 a         1138 c         1463 g         1888 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tt
//
LOCUS       DADOOS010000135         6588 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.136, whole genome shotgun sequence.
ACCESSION   DADOOS010000135 DADOOS010000000
VERSION     DADOOS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.136"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            104..2779
                     /locus_tag="J8C37_002354"
     CDS             104..2779
                     /locus_tag="J8C37_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAZ5305772.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2961..3200
                     /locus_tag="J8C37_002355"
     CDS             2961..3200
                     /locus_tag="J8C37_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305773.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3216..4589
                     /locus_tag="J8C37_002356"
     CDS             3216..4589
                     /locus_tag="J8C37_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5305774.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4593..6227
                     /locus_tag="J8C37_002357"
     CDS             4593..6227
                     /locus_tag="J8C37_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5305775.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6390..>6588
                     /locus_tag="J8C37_002358"
     CDS             6390..>6588
                     /locus_tag="J8C37_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305776.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2312 a         1064 c         1327 g         1885 t
ORIGIN      
        1 gagtgggata tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg
       61 tagtcataca aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac
      121 ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag
      181 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
      241 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
      301 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
      361 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
      421 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
      481 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
      541 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
      601 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
      661 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
      721 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
      781 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
      841 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
      901 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
      961 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
     1021 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
     1081 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
     1141 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     1201 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     1261 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     1321 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     1381 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     1441 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     1501 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     1561 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     1621 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     1681 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     1741 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     1801 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     1861 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     1921 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     1981 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     2041 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     2101 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     2161 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa
     2221 aattgaagca acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc
     2281 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca
     2341 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     2401 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     2461 aacattgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     2521 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     2581 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     2641 ttttaggcaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     2701 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     2761 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     2821 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca
     2881 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     2941 tgaaagaaag gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     3001 tgtatattca agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt
     3061 tcgaaataag aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg
     3121 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat
     3181 ggtatttagt tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa
     3241 cgtctgttta tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt
     3301 atgttttaga gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg
     3361 aaaaagcgga gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     3421 agggggtgat ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     3481 aatttattga gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc
     3541 ctgcttcttt ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     3601 cagaacaaca acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc
     3661 aacaacggca atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa
     3721 tccaacaaga agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca
     3781 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg
     3841 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     3901 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag
     3961 caaccttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     4021 atctgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     4081 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     4141 gctgccaagg aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     4201 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat
     4261 tagccgaaaa gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac
     4321 aatggattaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc
     4381 aagctcttca aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     4441 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg
     4501 tttgtcggat tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga
     4561 cacaaaatat tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg
     4621 cattgtatat tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac
     4681 aagaacaaaa tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg
     4741 ccgatgaagg aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     4801 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     4861 gcaatatgtt agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac
     4921 attcgatctc ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc
     4981 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc
     5041 aaagcttagt gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg
     5101 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     5161 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     5221 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     5281 tcttacgcaa taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa
     5341 ccaaacgacg gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga
     5401 ttatttcgga agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac
     5461 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     5521 gcgcaccgat ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga
     5581 ttcgatatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     5641 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     5701 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     5761 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     5821 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     5881 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     5941 ggatctttca acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactaatt
     6001 tggatcgttt tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta
     6061 tagagaaaat aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca
     6121 cgcatgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg
     6181 atacggctat ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc
     6241 aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt
     6301 tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa tataattgta
     6361 tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg
     6421 aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag
     6481 gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg
     6541 attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DADOOS010000136         6534 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.137, whole genome shotgun sequence.
ACCESSION   DADOOS010000136 DADOOS010000000
VERSION     DADOOS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.137"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(44..1420)
                     /gene="trmFO"
                     /locus_tag="J8C37_002359"
     CDS             complement(44..1420)
                     /gene="trmFO"
                     /locus_tag="J8C37_002359"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAZ5305777.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1389..3467)
                     /gene="topA"
                     /locus_tag="J8C37_002360"
     CDS             complement(1389..3467)
                     /gene="topA"
                     /locus_tag="J8C37_002360"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAZ5305778.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3589..4446)
                     /gene="dprA"
                     /locus_tag="J8C37_002361"
     CDS             complement(3589..4446)
                     /gene="dprA"
                     /locus_tag="J8C37_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAZ5305779.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4502..5269)
                     /locus_tag="J8C37_002362"
     CDS             complement(4502..5269)
                     /locus_tag="J8C37_002362"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAZ5305780.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5262..6122)
                     /gene="ylqF"
                     /locus_tag="J8C37_002363"
     CDS             complement(5262..6122)
                     /gene="ylqF"
                     /locus_tag="J8C37_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAZ5305781.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         1666 a         1438 c         1155 g         2275 t
ORIGIN      
        1 aaaagagcga gactatccgt ctcaccctct tcatggagtt tccttactca tttgggattg
       61 cagttttttc taattctgtt tttattgcct cattagcttt taaagctcgt tgtgcaattg
      121 cttcatatct tgcttttttg tctcgaatgc gctctggcag ttcaggaaac agaccatagt
      181 tggcattcat cggttggaaa tgttttcctt ccgcatgagt aatgtagtaa gccatacttc
      241 caatagctgt ttcttgtgga aactcgactg gttccatttc ttttgctaag cgtgcggcgt
      301 tgattccagc aagtagtccg ctcgctgcac tttcaacata gccttctaca ccagtcatct
      361 gacctgcaaa gaacagatct tcgcgtttcg ttgattggta agtcggtttc agcaattctg
      421 gcgaattcat aaagctgttt cggtgcatca caccatagcg aacgaattca gcattttcta
      481 acccaggaat catccggaaa acacgttttt gctcgcccca tttcaagtga gtctgaaagc
      541 ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg cagctggata acagcataag
      601 gacgtttgcc agttttagga tcttccaatc caactggttt cataggtccg aaaagcatcg
      661 ttttgaatcc tcgctgtgcc attacttcaa ttggcataca tccttcaaaa tatttttctt
      721 tctcaaaaga tttaagttct gccacttcgg cattgattag tgcctcatgg aaagctttga
      781 attcctcttc tgtcatcgga cagttcaagt aagctgcttc tcctttgtca tagcgggact
      841 tcaaatatac tttatccata tcgatcgtag ctttgtctac gattggtgca gctgcatcat
      901 agaaataaaa accatctgaa ccattgaatg ctttgatgct ttctgctaat ggatgagaag
      961 tcaaaggtcc tgttgctact acggtgattc cttccggtat ttcagtaatt tcttcgttta
     1021 tcactgttac taatggatgc tctttgactt ttcgggtaat tgtttcagaa aaagaatcgc
     1081 ggtcaacagc taaggcacca ccagctggca ctgctgtttc atctgccgaa gtgattacta
     1141 ccgaattcaa tcggcgcatt tcttctttca ataaccctac tgcattagcc aaactgtttc
     1201 ctcttaatga attggaacat actagttcag caaaattact tgtgtgatgc gcctcagtcg
     1261 atttgactgg acgcatttca tataaacgga cagggactcc tgcttgtgcg atctgccaag
     1321 cagcttcact tccggcaagc cctgctccaa caacatttac cattttcaat gaaagaacct
     1381 cttttctatt atttttggat gtcttcttcg taatctccat ttacacagac tacttgttta
     1441 ccgcctttga ctttcttctc gaccagatat ccgtcacatt ttggacaatt tctgcccact
     1501 ggtttatccc aagaagtgaa ttcacattct gggaaacggg aacaaccata gaatagtctg
     1561 tttttcttag attttcgttc aatgacttgt ccttcatgac atactgggca agtcacaccg
     1621 atttctttta cgattggttt cgtattccga caatcaggaa aattactgca agcataaaac
     1681 ttcccatatt tccctaattt tatcaccatc ggatgtccac agacatcaca atcgaatcct
     1741 gctggttcat ctttgatttg aattttttcg attttttctt ccgcattcgt cagttctttt
     1801 tcaaacggtt tatagaaacg atcaaccact ttgacccacg cttccgtgcc ttcttcaatt
     1861 ttatccaaat ctccttccat ttctgctgtg aaatggatat cgacgatttg agggaagaat
     1921 tcacagatta atgaattgac aatttctcct agttcagtcg gctcaaatct tttttgtgtc
     1981 aattttacat agtagcgtct ttggatcgta tcaagtgttg gtgcataagt ggaaggtcgc
     2041 cctaccccgt tttcttctag tgcacgaatc aacgtagcct cactaaaacg tgctggtggt
     2101 tgagtaaaat gttgttttgg ttcaatatca acagattgga ctacatcgcc ttcttcaagt
     2161 tctggaagaa tattttcttt ctcttctttg ccgtcatctc taccttccac atacacttgc
     2221 atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga agatgacacc attttgttct
     2281 aatgtcactt tcattgtatc taaaatagct ggtgtcatct gactagccac aaaacgtgac
     2341 cagatcaatg tataaagttt caattgatct ttatctaaat attgtttgag ttcatctggt
     2401 gtacgaagca cactcgttgg acgcacagct tcatgggcat cttgtgcgcc ttgagcattt
     2461 ttgatctttc ggtgatcatg tctagaaaat tcttttccat acgtatcttc aataaatgct
     2521 gcggcctctg cttttgctgt atcggcaatc cgggtagaat cagtacgcat gtatgtaatc
     2581 aagccgactg ttccttgttt acccaaagtg attccttcat ataattgctg tgctaccatc
     2641 atggtttttc ttgttcgaaa gtttaactta cgggcagctt cttgttgcag gctacttgta
     2701 gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt ctactttttt aacttcgaag
     2761 tctgttccat caattctagc agtgacttct tttacacttt gtgcatctgg taatttcttt
     2821 ttcttaccgt cgattcccca gaaatttgct ttgaatttct tgcgagcttt tttaaaatta
     2881 ccgtcgatgc tccaatattc ttctggaaca aattctcgga tctctttttc acggtcaata
     2941 ataattttta aagcgacaga ttggacacgt cctgcactta accctttctt gaccttacgc
     3001 cagagaatag gactgatcga ataaccaacg attctgtcta aggtacgacg agcttgctgt
     3061 gcatctacta aatctacatc gatcgttctt ggttccttaa aagctgcttt gactgcgtct
     3121 tttgtgattt cattgaagac cacccgattt ttttctttca gatccaaccc aagtaggtac
     3181 gctaaatgcc aagcaatcgc ttctccttct ctatccgggt cacttgccaa gtaaactttt
     3241 tgagcttttt ttgctgcggc tttcaggctt ttgatgacat cgccttttcc gcgtatagaa
     3301 atataatggg gttcataatt gttttcaaaa tcgataccca ttttactttt cggtaaatct
     3361 cttatatggc caacactggc cattacttta tagtttcgtc caagatattt ctcaattgtc
     3421 ttggcttttg caggtgattc tacaatcact aaatatttat acgccattac tgaatggact
     3481 cctttccatt gtcgtgttcc tactattaaa agaggattca tacaaatcgt agctaatcat
     3541 atccattcag aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt attctttaaa
     3601 ttctgccaag atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga tcaaatgatg
     3661 acatccggta gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg ttcttccaag
     3721 atccaatgct tgctgtgctg ttatcagaga gccgcttttt ttcttggctt caaagacaca
     3781 tgttccttta gacaaagctg cgataatccg atttctcatt ggaaaacaat gctttttcgg
     3841 gccggattga ggaggatatt cactcaaaag aagatgatca tttgcaatca cctcttgcaa
     3901 gtcacgattt tcagcaggat agacttgctc tatccctgtt ccaatgactg cgattgtccg
     3961 tccaccatga gagattgctg cttggtgacc gtatgtatct atccctttcg cgcagccact
     4021 gacgatcaca tatccagctt ctatcactgg ttttagtaaa taataaactg tttttttggc
     4081 taaagcagaa gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa gtaaagatag
     4141 atcaccatta taaaacagta caattggcgg ataggtcaag tgaagcagct gagggggata
     4201 tttgggatca aagcaagtga taaacgactg tttcgattgg attttgcccc agttagcgtg
     4261 aatttcctgc caacttttgc gaaacagctc ccgatgtttg gttattttac ctatcttaat
     4321 gatgtcttca aatccgattt ctttataagg atactgcatc atccagttag caagtgccca
     4381 ttttccttga ctactgattc cttttgtata agcaagtttc aataaatttt cttctatttg
     4441 atacatcata aaaccgcctt tcgtctctct ttctaataag atacgtaaaa gcggctgttt
     4501 tttattgtaa atattttttt acaggtgcaa aacttttacg atggattggc gtaattcctt
     4561 gtcgttcaag tccttctaag tgttcctttg ttccataacc tgcattatgc gagaaaccat
     4621 atccaggata aagcacgtca tattctttca tcaattggtc tcgatatact ttagcaatga
     4681 tacttgcagc agcaatcgac acagatcgtg catccccttt gattattttt tcctgctgaa
     4741 taggcaaatc caaggtcatc gcatcgatca acaaatagct tggtacaatg gacaattttt
     4801 ctactgcttg gatcatcgct aatttacttg cttcataaat attcacatca tcgattgtct
     4861 gctgatcaac gatcccgatc ccgattgcca gggctttttc ttgaacttgt tgataaagaa
     4921 gttctcgctt tttctctgat aattgtttgg agtcattcag ccctaatatg atctcatttt
     4981 tcggcaagat aactgcagct gccacgacag gtccagctaa tggtcctctt cctacttcat
     5041 caatgccggc aatccattgg tgaccttgtg cgtacccttg cttttcaaag caaaacattt
     5101 cttcatattt ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa gaagccagtg
     5161 cttgctgcac gcctttgcgt ggatcatttc ttaatgattc tagtgtagga tcatctattc
     5221 tctgtatgct tttcaatcgt tctttgatac tttggatgga ttcagtcatc acttttttgc
     5281 tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt ttccttgacg aatatcatgc
     5341 acgactaatt cgctgccacg atcatagtct tctttatacc cacgttttgc tgtaatatcc
     5401 atcagtaatt ccggtgccaa tttttcttct tgctcgattg ataaaccata acgatcgctg
     5461 attcttcctg aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa atcatctaaa
     5521 tggacaagct ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat ttcttcatct
     5581 tcaaatttgg gccataaaat ccctggtgtg tctaacaatt ccaactcatt acctaaacga
     5641 agccactgct gaccttttgt tacgccaggc ttatttcctg tctgtgcaat tttcttgcct
     5701 actagtcgat tcatcaacgt agatttccca acatttggaa tacctaagac cattgcccga
     5761 atagcgcgag gcttgatccc ccgagcagca tctcttgccc gtttttcttt caatgcttct
     5821 ttagccgcag gtactagacc tttaattcct ttactttctt gtgccgtgat agctaatgca
     5881 agatggcctt gtttacgaaa atattcttgc cacagctttg tctgttgtgg atctgccaga
     5941 tccgctttat tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc tagcattgga
     6001 ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc cacatatttc
     6061 attttttcag aaacttctcg tcgggctttt gccatatgtc ccggaaacca ttggatggtc
     6121 attgttctct ccattccttt cataccggaa tattccccgg ctttacttgt ttttttcttt
     6181 ttcaatataa attcactttt ttattataaa gaaattccat ctattagtta agtgaaagtg
     6241 aaccagtcct cattctatca catcttcact tttttgatga tgcaaatttt tattttggtt
     6301 tctatatatg aatctaaatt tttgacagcg tttcctaaat aagagatgat agacgaacaa
     6361 aacagaaagg agacgaaaca aatgaatagt atggtttttg ttaattttcc tgttgctgat
     6421 gtcaatcgtt ccgttgcttt ttatgaaaag ctgggtttta aaaaaaatga agaattttct
     6481 actgaagaag ctggttcaat ggtatgggat gacacttttt ggataatgct tctc
//
LOCUS       DADOOS010000137         6425 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.138, whole genome shotgun sequence.
ACCESSION   DADOOS010000137 DADOOS010000000
VERSION     DADOOS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.138"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(131..754)
                     /locus_tag="J8C37_002364"
     CDS             complement(131..754)
                     /locus_tag="J8C37_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305782.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(913..2310)
                     /locus_tag="J8C37_002365"
     CDS             complement(913..2310)
                     /locus_tag="J8C37_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5305783.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2342..3313)
                     /locus_tag="J8C37_002366"
     CDS             complement(2342..3313)
                     /locus_tag="J8C37_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5305784.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3687..4112
                     /locus_tag="J8C37_002367"
     CDS             3687..4112
                     /locus_tag="J8C37_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305785.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4600..5301)
                     /locus_tag="J8C37_002368"
     CDS             complement(4600..5301)
                     /locus_tag="J8C37_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAZ5305786.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5317..5757)
                     /locus_tag="J8C37_002369"
     CDS             complement(5317..5757)
                     /locus_tag="J8C37_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAZ5305787.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5757..6332)
                     /locus_tag="J8C37_002370"
     CDS             complement(5757..6332)
                     /locus_tag="J8C37_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAZ5305788.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1825 a         1333 c         1024 g         2243 t
ORIGIN      
        1 ctcttgatac acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat
       61 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
      121 cctctgtttt tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa
      181 ttgaggcttg attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac
      241 ttgttcctgt cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg
      301 tctttttcca ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag
      361 cggtgatcac ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca
      421 aaacggtcaa atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt
      481 catcgatatt aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc
      541 ctttttcgta attggcagtt attttatact ccgcccacca ataagttgag aattcttttt
      601 tgaaattatc tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat
      661 ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata
      721 accaaaagag aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt
      781 gactattcta ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta
      841 aataattgaa cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc
      901 aactattctt tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc
      961 cgattttttg attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg
     1021 attcttatat gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa
     1081 acaattggca ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc
     1141 gatccgataa tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac
     1201 acccataccg ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat
     1261 caaaatatca tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg
     1321 accaggcatt tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat
     1381 tggtgtttct tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac
     1441 tgtattttct ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc
     1501 ttttactatc tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga
     1561 acgattgaag aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc
     1621 tcttggatag cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc
     1681 atggtaagat ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc
     1741 ttttttcata tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg
     1801 aacgattggt tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca
     1861 acttttggca tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc
     1921 ttgcagatac atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc
     1981 aatcaactca tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga
     2041 ttcatcaggt atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg
     2101 tttcatcaac tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc
     2161 tacttgatca aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc
     2221 ttttccatcg cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca
     2281 aaaatttgct ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt
     2341 tttagttgat tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt
     2401 cttttttgat tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg
     2461 gcgtgatcgt gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac
     2521 tgaacgcaat atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa
     2581 tcgacatatt gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg
     2641 ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg
     2701 gatcattttg gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt
     2761 gagtggcagc aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt
     2821 ttgctacact ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac
     2881 aattcaatgc gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt
     2941 cttcggataa gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt
     3001 gttcaatttt ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt
     3061 cagctactct ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta
     3121 tcaaaagtcc aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt
     3181 tatagtccaa cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct
     3241 gaccattcaa gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac
     3301 ggatcgatgc catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt
     3361 cgattatgat gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa
     3421 agtcttcagt agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg
     3481 gttcattttt aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg
     3541 aaagtggcgg attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt
     3601 gttttgtgcg tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct
     3661 ttatcctatc aaatggggga agtattttga agaaattaat cgtactattt attttgtctg
     3721 cgggactttt gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg
     3781 aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata
     3841 cgctaaaagt acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa
     3901 aaatggaatg gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag
     3961 aaaaaacagt gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag
     4021 gaaaagaaaa taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga
     4081 aggatgagga tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc
     4141 tccccttctt ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg
     4201 atcgatagtc ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat
     4261 gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa
     4321 acatttgaat cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca
     4381 agcaaaactc actatggctg atttttatga acttcaaact cagcttcttc ctttgatcaa
     4441 gcttcatcag taaaacaaac agtggatcat ttcagtttac aaatcaaaga gtaaagattt
     4501 atttatttca aatatttgaa tataaaataa ccaaacgata gaaaggctta ttctacatca
     4561 cgtagtaatt tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt
     4621 tgcatgctgg ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt
     4681 ttcttggatc tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt
     4741 atccccatcc acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg
     4801 ggtgttcaac gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc
     4861 acgcccacgt tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat
     4921 caaatcaggc cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa
     4981 gctatcgtac tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa
     5041 caacaggcta tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt
     5101 tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc
     5161 aggatttgaa taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt
     5221 agaatcaatc tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc
     5281 tgaaatcaaa atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt
     5341 ttcaacaggc aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt
     5401 atggagactt tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat
     5461 cggttttttc aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg
     5521 atgctcaaag tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt
     5581 ttcgtatact tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg
     5641 acgtggagag tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact
     5701 tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat
     5761 tttgcctcct tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt
     5821 tccaatggat tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct
     5881 tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt
     5941 ccgattggct tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc
     6001 tccaatgtac ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat
     6061 acctttcttt catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat
     6121 tcagtttcaa aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact
     6181 gttttactaa tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct
     6241 tcttgtgcca tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca
     6301 aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct
     6361 atcttcattc tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg
     6421 tatat
//
LOCUS       DADOOS010000138         6392 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.139, whole genome shotgun sequence.
ACCESSION   DADOOS010000138 DADOOS010000000
VERSION     DADOOS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.139"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(43..336)
                     /locus_tag="J8C37_002371"
     CDS             complement(43..336)
                     /locus_tag="J8C37_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAZ5305789.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(408..2135)
                     /locus_tag="J8C37_002372"
     CDS             complement(408..2135)
                     /locus_tag="J8C37_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAZ5305790.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(2349..3275)
                     /gene="trxB"
                     /locus_tag="J8C37_002373"
     CDS             complement(2349..3275)
                     /gene="trxB"
                     /locus_tag="J8C37_002373"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAZ5305791.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(3420..4853)
                     /gene="ascB"
                     /locus_tag="J8C37_002374"
     CDS             complement(3420..4853)
                     /gene="ascB"
                     /locus_tag="J8C37_002374"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAZ5305792.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(4853..6256)
                     /locus_tag="J8C37_002375"
     CDS             complement(4853..6256)
                     /locus_tag="J8C37_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAZ5305793.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         1679 a         1489 c         1096 g         2128 t
ORIGIN      
        1 cttcgttgtc acactccagt gtttatctat tttcacctat cgttattgat gttccacatg
       61 tttgagtgtc tggttcaaaa tatccagtac atgagaatca tcaagactat acaaaatcgc
      121 ttttccctcg cgatgagact tgacgacatg attttctctt aataaacgta gttgatgcga
      181 tacagcagac tgctccattt ccactgcttg ggtgattgct gtcacatttt gttccccgtc
      241 tttcagatat agcagaattt taagacgagt gggatcactc aacattttga ataattgact
      301 gactttctct acttttgatt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa
      361 agactgactt ctaaagttta ccactttttc catcagtctc aagtttttta ttcttctgtc
      421 atctttttga taatcgcttc ataagaggct actttttcgg ctgcgtcttc tgtagagctt
      481 ccttttacac caatataaaa cttgattttc ggctcagttc ctgaagggcg gatagcgatc
      541 cagccatttt cttccaatgt atacttcaat acgtcggatg gcggtgttgt tagtttttct
      601 acgtttcctt cacggtctgt tctcgtcaat tctttaaagt cttctgtttg aataactttt
      661 gctccggcaa attctgtcgg tgcttcttta cggaattttt ccatcaaagc agagattttc
      721 gcagaacctt cttgtccgct cattgtcaca gaaatcgttt tttctgcgaa atagccatag
      781 gtttcaaaaa tcgtttgcaa gccgtcatac aacgttttgc cttgtttttt atagtaagct
      841 gctacttctg ctaataaaag caatgcttgg atcgcgtctt tatcgcgaac aaaaggttta
      901 actaaataac cataactttc ttcgaatcca aacataaaag tatgtgaatg gtcttcttca
      961 aattgttgga ttttttcagc aatgaattta aaccctgtca acacattgaa catggtagca
     1021 ccgtagtgtt ccgcaattgc tgtaggcaat tcgctggaaa cgattgattt caataccgct
     1081 gcgttttctg gtaaagtgcc tcttgattga tgtgcttcca aaatatattg agcaaacaat
     1141 gctccgattt ggttcccagt caatacttca taagaaccat ctggcaaacg aaccgctgcg
     1201 cctaaacggt ctgcatcagg atctgttgca atcagcaagt cagctccttc tttttctcca
     1261 agtcgtatag catattcaaa agctgaatgt tcttctggat ttggcgattt gaccgtagtg
     1321 aaatctggat ctgccacagc ttgttcggga actagtacga attgttcgaa tccagcttgt
     1381 ttcaacgcac gttcgcccaa cattttccct gttccatgaa gtggcgtgta cacaagtttc
     1441 aaatctttcc ccattgtttc aaccaattca cgatcaatcg tcactgcttt gatttcttct
     1501 agataagcag cgtccacttc ttctccgatg atcgtgatca acccgctatg tttagcttct
     1561 tcgtcagata atacttcgac ttttagcgga ttgctgactt cacggacata tttcgtcaat
     1621 gcatctgcgt ctgcaggagg catttgtccg ccatcttctc cgtagacttt atatccattg
     1681 taagccgctg ggttgtgaga tgcggttacc atgattcccg caaacgtctt caaatgacga
     1741 actgcaaaag aaagctctgg tgttggacgc agactttcga atacataaga cgggatattg
     1801 tgatgtgcta gtgttttggc tgcttccatt gcaaattctg gtgactgatg gcgtgagtca
     1861 taagcgatag cgacacctcg gcgttttgtt tcttcacctt gtgtatccat gaaacgtgct
     1921 agtccttctg ttgcttgtct taccgtatag atgttcattc gattgatccc aggtcctaag
     1981 attccccgca tacctgcagt accgaattcc aacggtgcat aaaaagcatc ttcaagtttt
     2041 tcaggatctt cacccagatc atttaattgt ttttttaagt tttcttctaa attttcttca
     2101 tgtacccatt gttcatacac ttgttcccaa gacatgaaaa taacacctct ttctgaaata
     2161 tggttcttaa taagtatagc attcacttat tagatatgaa agtatgagga ccaaaaaacg
     2221 cttaaatcaa gaaaggattc ttttatcaaa aggtgttgga cgtgttcgca taaatcagat
     2281 tcagtaaatt tcacgcgaaa aaaaagagta aaagaacaat tgaacccgtt cttctactct
     2341 ttttaaactt atgctgttgt ttcttctaaa gactccaagt atttatacac ttgttgtcca
     2401 gcaataccgc cgtcaccaac agctgtagtg atttgtcgca aatctttttc acgaacatct
     2461 ccgatggcaa aaacgcctgg catattcgtt ttcatttcgt tgtctgttgg aatccagcca
     2521 gcttcgttcg tcaaacctgc ttctctaaaa ggtgcagtta atggatccag tccaacatag
     2581 atgaatactc cgtctgctgc taaatcaaat tcattgccgg ttttgacatt acgtgctttc
     2641 acacctgtca ctaccatttc gtttccaacg atttcgtcaa cgactgtatc ccacataaag
     2701 gaaatttttt catttgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct
     2761 ctacggtgga cgatcactac ttcagaagca aattgtgtca aatagatggc ttcttctact
     2821 gctgagtccc cacccccgat gactactaga cgtttgttac ggaaaaacgc accatcacag
     2881 actgcacagt aagaaacgcc tcgtccagca aattcttcct cgccaggtac gcctaattta
     2941 cggtggatac atcctgttgc gataatgact gtttttcctt cgtaactttt atcttcacag
     3001 atgacttctt tatggctgcc gtgatccttg atatcttgaa cgattccata agcattttct
     3061 gtcccaaatc ttgagacact ttcatacatc ttataagcta gatcggggcc catgatcgaa
     3121 tcgaatccag gataattttc gatttctgcc gtattgttca tttggccgcc aggggcacct
     3181 tgttcgatca tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca
     3241 gcaggacctg cgccaataac gattacatca tacatatgta gattctcctt tacttaatca
     3301 agtaagcata ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga
     3361 atagctttat tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat
     3421 caatcgttcg aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat
     3481 tttttctttg aacgggctaa tgtgccattt ccttggttat cacgatcgac ataaatgaat
     3541 ccgtaccttt tcttcatctc accggtacca gctgatacta aatcaataca tccccaagtc
     3601 gtatagccta atagatcgac tccgtctagt tctactgcat ccttcatcgc ctggatatgc
     3661 gctgccaggt aatcgattcg gtaatcatct tccacataac cattttcatc tggcgtatcg
     3721 actgcaccta agccgttttc tacaataaac aatggttttt gataacgatc ataaatatca
     3781 ttcatcgtta ttctcaagcc taatggatca atttgccacc cccattcact tgcttgcaga
     3841 tatggatttt tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtcg
     3901 gtagtcgaaa ctcgggaagc ataataagaa aaagagataa aatcaacagt atgttctttc
     3961 agcaatgcgg catcccctgg ttccatttcg atctgaatgc catttcgctc catttctttc
     4021 aatgcataag cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga
     4081 tctgattttc tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct
     4141 gctgccagca tacagcctac tttgttctcc ggatctactt catgagcaat cttcgttgcg
     4201 atggcacttg caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct
     4261 cctttttcaa aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca
     4321 ttgaatgtca gccaatattt gacaagtcct ttatagcgat tgaagatgac tcgacacaga
     4381 ttttcataaa aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc
     4441 attggacaat caaaatgcgt aatcgtcaca agtggctcaa ttccgtgctt ttggcattct
     4501 ttaaaggcat cctcataaaa tttcaagcca gcttcattcg gctctttctc atcccccatt
     4561 ggaaaaatac gactccaggc gatggacatt cgataagtct tgaaccccat ttctgcaaac
     4621 aaagcaatat cttctttata acgatgatac atatcgattg caatttttgc tggataaaaa
     4681 tgatcctcat caaattcaaa catttttttc tttccggtga taacaggaaa acggtctttt
     4741 ccgattggca caacatctac gtttgctagt ccgcgtccac cttcatcata accgccctca
     4801 cattgattgg ctgctgtagc accgccccat aaaaaatctt ttctgaatcc cattatactt
     4861 cctccattat atgtgtatta cctgataatt ctcttgccag ttcatcttga acttcgattc
     4921 cttcttcttt taatcggctg attgtctcct tgaattgcgg taaatactgc tggtgtgcaa
     4981 taaacaactc atccatgatc cgttttgctg tctttcctga tcgtataagt ggattgatcg
     5041 taaacgcctg taaagctgtc ccataatcac cagtcacagc tgcttcgatc gtcagcaatt
     5101 ccatattctt cattacttgc aaccagcctc tttcagcagc aggaagagaa ccaaacgcta
     5161 catttctcgc tccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca
     5221 ggtcaggaac agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat
     5281 agattgaagc aatcgtttca caggcagcgt ctgaataata agcgccacct cgtttttgca
     5341 attgttcagg tttatggtct aattctggat ctttgtataa ttcaaatagc tctgcttctg
     5401 tttctttcac ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca
     5461 gcatttcttc ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt
     5521 ttaattgttc tttgaaaaac gggatatcaa agatattttt cggtaatcca ttctcttcat
     5581 cgtataaacg atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt
     5641 gccaatggaa atgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa
     5701 gcattgctgg ttctgtcatc atcgccatca ctggcacatt gcataatcca atcactttat
     5761 cccatttgcc atatctgata actgcttctg tcaccatccc acttgggttc gcgaaattga
     5821 tcagccaagc atttggacat aaacgcttca tgtcctcaac gattgccaaa acgactggaa
     5881 cggtacgaaa agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat
     5941 aataactagg gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag
     6001 tcgtaacaaa atccgcatct ttcaatgctt cttcccgatc cagagttgca tagatcttga
     6061 cttcatacgg agaagcatcc cacatacgct gagacatctg actaacgatc tcaagctttt
     6121 cttttccttc ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt
     6181 tgataaatcc ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt
     6241 tgattccttt tgccatgttc tcactccttt acttttttac actttcatta tcttttttgt
     6301 aaacaaaaca aacaatagaa atctgtttct ttccagtaac atttacaaaa tttactgttt
     6361 tctagtggat ttttgtaaat atcttgtcaa aa
//
LOCUS       DADOOS010000139         6303 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.140, whole genome shotgun sequence.
ACCESSION   DADOOS010000139 DADOOS010000000
VERSION     DADOOS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.140"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            334..714
                     /locus_tag="J8C37_002376"
     CDS             334..714
                     /locus_tag="J8C37_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAZ5305794.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="J8C37_002377"
     CDS             696..1610
                     /locus_tag="J8C37_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAZ5305795.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1570..1848
                     /locus_tag="J8C37_002378"
     CDS             1570..1848
                     /locus_tag="J8C37_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305796.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            1855..2544
                     /locus_tag="J8C37_002379"
     CDS             1855..2544
                     /locus_tag="J8C37_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305797.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="J8C37_002380"
     CDS             complement(2665..2751)
                     /locus_tag="J8C37_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAZ5305798.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="J8C37_002381"
     CDS             3427..4377
                     /locus_tag="J8C37_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAZ5305799.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="J8C37_002382"
     CDS             4398..4931
                     /locus_tag="J8C37_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAZ5305800.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="J8C37_002383"
     CDS             5567..6100
                     /locus_tag="J8C37_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305801.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DADOOS010000140         6283 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.141, whole genome shotgun sequence.
ACCESSION   DADOOS010000140 DADOOS010000000
VERSION     DADOOS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.141"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..439)
                     /locus_tag="J8C37_002384"
     CDS             complement(<1..439)
                     /locus_tag="J8C37_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAZ5305802.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEK"
     gene            complement(569..1279)
                     /gene="budA"
                     /locus_tag="J8C37_002385"
     CDS             complement(569..1279)
                     /gene="budA"
                     /locus_tag="J8C37_002385"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAZ5305803.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1292..2956)
                     /gene="alsS"
                     /locus_tag="J8C37_002386"
     CDS             complement(1292..2956)
                     /gene="alsS"
                     /locus_tag="J8C37_002386"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAZ5305804.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3164..3826)
                     /locus_tag="J8C37_002387"
     CDS             complement(3164..3826)
                     /locus_tag="J8C37_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAZ5305805.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3836..4651)
                     /locus_tag="J8C37_002388"
     CDS             complement(3836..4651)
                     /locus_tag="J8C37_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAZ5305806.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4912..5793)
                     /locus_tag="J8C37_002389"
                     /pseudo
     CDS             complement(4912..5793)
                     /locus_tag="J8C37_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331511.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5814..6191)
                     /locus_tag="J8C37_002390"
     CDS             complement(5814..6191)
                     /locus_tag="J8C37_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAZ5305807.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
BASE COUNT         1896 a         1347 c          992 g         2048 t
ORIGIN      
        1 atttttctag aaatgcttga tcgatttcat catacccttt cgttactccg caatcgatat
       61 ctaaaacacc gattaaatta ccttctttta ccattggcac tacgatttct gattgtgcgg
      121 ctgaatcgca ggaaatgtaa tttgcatggg ttttgacatc atcaacgatc aatgtttctt
      181 gtttttcagc tgattctcca caaactcctt ttcccatctt gatacgtgtg caagaaactc
      241 gcccttggaa cggtcctaaa atcaattcat taccatcgaa taaataatat cctgcaaaga
      301 cagtctcagg caaagtctca ttcaacaatg cagatgaatt tgctaaattt gctaaccagt
      361 tatcttctgc ttctaataat ccttgttgct gtaataataa aatttcgtat gcatcttttt
      421 tctgtttttc tgtccacatg ataaaaaccg ctcctttttg atctatggag aagattatag
      481 ctcagttttt ttgtaaacgc taggtttcct cctcaaaaaa acgagatacg tcaggttaat
      541 caccttgaac gcatctcgct gtctttttct attctgcttg ttggatttct gctgctaaat
      601 cagcataatc aatcgtttcg tgaacaaaag tcggatcatc aactggaaaa tgctgtacaa
      661 gggacgagat gcggctaatc tcaacttttc cttcagccac ttcaaaatcc attacatgtc
      721 cgccaaatgt atgtgtatca tcgataaaat gcaggtgaaa tcccgctgca gcaactcctt
      781 ggaataattc aggcgtaaag aacccgacta atgttccttg gatattgctt tgttggaatt
      841 ctggttgaga ctctgaaatt tttgctagtc ttctgtaagg tttttcttgc ttcggcatga
      901 tacggatgtg catatttttg aataatccag ttatcttcac tgccaaaaat aggttttccc
      961 cggcttctct tactaatatg tcttttttca attgttctcc atctgcagtt tttgagacag
     1021 aaaaactagt atctggcgta aaatctgtaa ttgctgcata gggggtaagt tcacttccat
     1081 ctaattgggc aactgagcca tctgatcttc cttgataagc tacgccatct aaaaagatga
     1141 cttcaccatc aagcccatgc aatgttccca atcccagagt tcctttttct aatatatgag
     1201 caatcgtttc tgttccatcc atcaagccgg ccatcaaagc tcctaatgtc ccgtgctggt
     1261 ataagatttt ttcagtcatg tcgacacttc cttaatagaa ttgatctggt aagattgttt
     1321 ctcccaattt ctcattatca cggtaatcaa tcggaatatc aataattacg ggtccttctg
     1381 tcgcaaatcc ttcttctaaa gctttttcta attcctctac acttgtagct cggattcctt
     1441 tagcgccaaa agcttctgca tatttcacaa aatcaactgg accaaagtct acacctgacg
     1501 cccgctgata tttcattttt tcttggaact ctaccatatt gtagtgtcca tcattccaaa
     1561 tcaaatgaac gatattcaat tttttacgta ccgccgtttc taaatcctgg gcagagaata
     1621 aaaatccccc atcgccggaa actgatacga tttgtgtatt tggacgcacc aatgcagcag
     1681 aaatcgccca tggcaatgca acaccaagcg tttgcatacc attactgaat aacaaatgac
     1741 gaggttcata acttctaaag tggcgtgcca tccaaatata atgactacct acatctacgg
     1801 taacagtcat atcatctgtt acttttgact gaagcgtgtt aataacttct aaaggatgca
     1861 agatacctgc ttctcctttt gattgaacaa tgtcccgctc cgttaatttt gcttgaagag
     1921 atgctaaata ttcttttgca tcttctgaca cttgttgagg ctctaatgaa cctgtcaata
     1981 aatcaagagt ggctgagata tcaccgatca attctctttc aggctgcata aaaggatcga
     2041 tttcagctgg cgcttcatcg atcacaatga tccgcgcatc tttttccgca ttccagtttc
     2101 ttgcttcata ttcgattgga tcatagccaa ttgcaataac tagatcactt cgtttcaaaa
     2161 gcatatctcc tggttggttt ctaaataaac ccacacgacc aaagaaatgc gcttctaatt
     2221 cacgtgagat cactccagct gcttggaatg tctctacaac aggcaatgct gtcttttcaa
     2281 ctaattgcct gatggctaac gtttcttttt cacttgatcc tcgcatacct actaatagta
     2341 ccggtagttt tgcttctcta atgcgttcag ctaggtaacg gatatcatct gcagaagcag
     2401 aacctaattt aggatcgctc aatggcttga tgacattacc ttggacaggt gcatccacta
     2461 catcttgcgg gatactgata aagctcgcgc ctttttttga actcttagcc atacgataag
     2521 cattggcaat cacttctgaa attgtttctg gatcttggat ttctgcactg tattttgtga
     2581 ttggctggaa cagtgctgca ttatccatgc tttgatgggt caacttcaat agatcgcttc
     2641 gttttacttg tccggcaatt gctaaaactg ggtcaccttc tgctgtcgct gttaccaaac
     2701 ctgtagctaa gttactagca ccaggaccac ttgttgcgat aactactcct ggctctcctg
     2761 taatacgtcc gattgcttgt gccataaatg cagcattttg ttcgtgacga gtcacgatta
     2821 attcaggacc ttgatcctct aattcattaa atacaccatc gatttttgca cctggtatac
     2881 cgaatacata atcaacatga tgattaatca aactttctac tacagatttc gaaccttgtt
     2941 tcattttttc agccattgaa catcgtcctt tttatgcttt atttttaatt attagttaga
     3001 aataatcaca aataagtgat acagtttcta aacctgtttc atacaaaagt atagcacgtt
     3061 ttatttcatt tgtttagcca tatgtttcaa aaaatgaaaa agaatgacag tttttacata
     3121 aaaaaaactg tcattcttaa ttgaaagttt caatttataa agtctacaga tccatagttc
     3181 tcatcaattt acgattgttg aagtaaatca aaccaaatac gatagaagga cctagtagaa
     3241 tacaccattg gggctttacg aaaaagaaga gaaacaaact tgtcaatagt aaaaaatcta
     3301 gtaaactcac ataccatttt ttcaacgtat agtagaacgc tatccgtaga acttgatttg
     3361 gtttcgacgc tggatttctc acctgaaaat accagcagtt ggtgctaaag gaaactgata
     3421 aacaagcaag taaaacaaac aacggaatca accattttcc ataagaaaaa cgtataaaaa
     3481 agaaacaatc aaaaatcatt atcacactaa ctatccaagc aaatagccaa taaaaaaatc
     3541 cctttagcca aaattggcga aacatcttta tgaacaaacg tactggtgaa aaactatttt
     3601 cttttgctaa ttcttctgtc atacctatca tcacgatgca acctggacca ataaaaagta
     3661 aagaaaaaat aaaggaaaag acattccttc catctatcga caaaaggaac gcagctgcta
     3721 tatacaagat attgacacac caaaaacaaa ggctacttgc caatgattga tacaaccaat
     3781 aaaagaattc catataaaca tttttgtcaa aacgctgctc tttcataatc acctcctatt
     3841 ccttcattcc tgatgaagcg atcccttcta caaaatattt ttgcatcgct aaaaatataa
     3901 aagcaacagg aagaacagaa acaactgagg cagccatgat taatgcgtac tctgcatcaa
     3961 atagcccaat aaacattttc aaccctaact ggattgtttt attttccgta gaagacaaat
     4021 aaatgaatgg ccccatataa tcgttccatg tattaacgaa tgtgatgatc gtcaaactgg
     4081 caatcgccgg ttttgtaaga ggtaaaatga tcttgcgata gattccccac tctgataagc
     4141 catcgatccg cgcactctca cataaagaat ctgggatact actgtaatat tgcttcaaca
     4201 agaacacacc aaaagcattg aacgcttgta atagcaccaa cgaccacaac gtatctgtta
     4261 aacccacttt tctcatcata atgaactgcg ggatcatata agactgccaa gggacagcaa
     4321 tcgtgccgat atatgctaaa aataaaatat cgcgcccttt aaaatttagt ttagaaaacc
     4381 cgtaagcagc aaaactagaa gtaaacaact gaatcaacgt gatcacaacg cttaaaaaag
     4441 ctgtattctt aaaaaatgtc actaaaggaa tacgagacca aataaccaga taattctcta
     4501 aatgccaagt tttagggatc cattgaatcg gaatcgtgaa cacttcatta tttgctttga
     4561 atgaagcaga aaccatccat aaaaaaggaa tgatcgtcaa taaagctaat acaatcagca
     4621 gaatcatacc caaccattga ataaagggca tgttttttga acgaattttt gctttttttc
     4681 tttcatttcg aacacctcca aagttataat cttcatttct ttgtaatgat tacaatccag
     4741 aacaacggtt attatagctg attcatttcc gttcttctta tttttgtaag cgcttattta
     4801 acaagttatg taaatctctt tatttttttg atttaaaaat gaaaaaatac aaaagcaaga
     4861 ctgtcatacg tctcgctttt gtattgaaat ttcttcgatt agagatactt attactgttc
     4921 aaaagcttct ttgaaacggt gaatcaattc attgactaca ggtgataaaa tccgattctt
     4981 tttccagact agcatgcagt tcgtttcaat cattgggtca agcggaacga atgtcactcg
     5041 ctttgaatga tgacttacag caccttctat gactaaagca gcacctacct gattctcaat
     5101 tagcgcaaat acattgaaaa tcaaattgta cgttcctgtg atcagcaact gctccttttt
     5161 atgcctaacc agtcagcaaa cattcgctgt atttctggac gctttgagat caaaagaggt
     5221 acattcgcaa agtcttcttt tgttatcacg tctttttttg ctaaaacaga attttctaaa
     5281 atgagcaatc cccatttttc ctttcttggt aacaaaatcg tttcgtattt ctctgtatct
     5341 actggatcta gcaaaatcgc taaatctaac aggctttttt ctagtcactc cgtgatatca
     5401 tcacttgttc cgctgtggat atgaaattga acgctgggat aatcacggat caacttttct
     5461 agtaatagtg ccaatgtatt cgaactatca gcttccacac agccgatcgt aaatcggccg
     5521 ctgaataatt gtttttttct gttctcgaat tcttgagtgg tttgattcgt taaatctaaa
     5581 atttcttctg ctcggctttt ttagaaaata acctgcttct gtcaagatca ttttgcggtt
     5641 ttcgcgtaca aacagttctg ttcctagtta ctcttctaac tccttcagtt gtcggcttaa
     5701 ggttggttgt gtaagatgta gaacttctgc ggcttgagag attgttcctt cttctgcaat
     5761 tgtccaaaaa tattttagta agcgtaactc catagcgatg ctccttgatt tttttacttg
     5821 atacattcca ttttatttct agataacaca cgatcatatt tcttgatttt tccttgtaaa
     5881 cgttccaaac attcttgcat ttcagcaatt tttttcgtca aaagttcact ttcttcaatc
     5941 aaaagcttac gccgctcaaa tacagttgct tctccttcgc tagataggcg aatatattca
     6001 atcaaagact cgatcgataa tccggcattt cgcatacaga tcgtaaagtc gacccatttt
     6061 aggtcttctt ctgtataatc acggattccg ccttttgtcc gattaactgg tggaatcaac
     6121 ccgattcgtt cataatagcg caatgtatcg gccgaaatgc ccttttcttc actgacttgt
     6181 ttgatattca tctgatcact ctttctctta atttcttgcc actttactta tgtattttat
     6241 ttattctaca actttaagtg tactccaagt caatactcat ttc
//
LOCUS       DADOOS010000141         6173 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.142, whole genome shotgun sequence.
ACCESSION   DADOOS010000141 DADOOS010000000
VERSION     DADOOS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.142"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            725..811
                     /locus_tag="J8C37_002391"
     CDS             725..811
                     /locus_tag="J8C37_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAZ5305808.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="J8C37_002392"
     CDS             complement(932..1621)
                     /locus_tag="J8C37_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305809.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="J8C37_002393"
     CDS             complement(1628..1906)
                     /locus_tag="J8C37_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305810.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="J8C37_002394"
     CDS             complement(1866..2780)
                     /locus_tag="J8C37_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAZ5305811.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="J8C37_002395"
     CDS             complement(2762..3142)
                     /locus_tag="J8C37_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAZ5305812.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="J8C37_002396"
     CDS             complement(3592..3879)
                     /locus_tag="J8C37_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAZ5305813.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="J8C37_002397"
     CDS             complement(3899..4123)
                     /locus_tag="J8C37_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAZ5305814.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4781)
                     /locus_tag="J8C37_002398"
     CDS             complement(4578..4781)
                     /locus_tag="J8C37_002398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305815.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="J8C37_002399"
     CDS             complement(4763..5296)
                     /locus_tag="J8C37_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAZ5305816.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="J8C37_002400"
     CDS             complement(5317..6159)
                     /locus_tag="J8C37_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAZ5305817.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DADOOS010000142         6133 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.143, whole genome shotgun sequence.
ACCESSION   DADOOS010000142 DADOOS010000000
VERSION     DADOOS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.143"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            100..2541
                     /locus_tag="J8C37_002401"
     CDS             100..2541
                     /locus_tag="J8C37_002401"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAZ5305818.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2871..3575)
                     /locus_tag="J8C37_002402"
     CDS             complement(2871..3575)
                     /locus_tag="J8C37_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305819.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3762..4007)
                     /locus_tag="J8C37_002403"
     CDS             complement(3762..4007)
                     /locus_tag="J8C37_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305820.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4024..4875)
                     /locus_tag="J8C37_002404"
     CDS             complement(4024..4875)
                     /locus_tag="J8C37_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAZ5305821.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5041..6045
                     /locus_tag="J8C37_002405"
     CDS             5041..6045
                     /locus_tag="J8C37_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAZ5305822.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1964 a         1063 c         1297 g         1809 t
ORIGIN      
        1 taaggattgt taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt
       61 acagcaaaga atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata
      121 gatgctgcaa gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt
      181 gaattaggca cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa
      241 atcgatcaag taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga
      301 cagattgctt tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt
      361 tgcggatctg gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca
      421 gaacttgtga tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat
      481 cagtcagaaa caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact
      541 gacgcatttt caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct
      601 gtgagcagag aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct
      661 gtcgaagccg gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg
      721 ttatctgagg atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt
      781 acagtattct cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt
      841 gcctctgtgg tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta
      901 gcaaccatca aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca
      961 atcaaagcca ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt
     1021 tttgagatca atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta
     1081 gatgaagaaa aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca
     1141 agcggtgcac gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac
     1201 ggtattgctt cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat
     1261 atggagcaaa cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa
     1321 cggcgcagtc aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag
     1381 gaacaaacac tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg
     1441 gaaatcccaa tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg
     1501 atggcaacag aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga
     1561 aatatctgcg cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa
     1621 tcggagtatc aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt
     1681 gcaaatgaaa gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact
     1741 cgggaattca tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa
     1801 gatgcaatgg gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg
     1861 gagtggttcc ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca
     1921 ttggcttctg cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt
     1981 gaacaaattg ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt
     2041 gcagcaacgc acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga
     2101 aatgatacac gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac
     2161 caaggattaa cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct
     2221 ttggctgttg ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta
     2281 gctatgttag atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta
     2341 gctcaaaacc tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg
     2401 ggacttcaag ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa
     2461 gttgcgaaaa aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg
     2521 gaaaaaatcc gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag
     2581 ttattttatt tactgcagat cttgcttttt tctctatcag aaaatatgtt tattattaga
     2641 attttttata gttaaggtta ggcatcttaa gaaatcaggc aactccaaga tatcttcagg
     2701 tagaagagaa gtcgcctaac attgtaggat cataggtgat ttgttggaca ggtcaagctg
     2761 atatttgagc ctgatgttag aaggaatttc tttttacttg ctgtagaaat tattatatga
     2821 ctaaaaaggc tgggatttta aatcccagcc ttttatgaca aatagaattt ttatcgatca
     2881 gtaatcgctg tcgctgcaaa ttgttcgatc aaaggaatca aagtcaattt gactttgtat
     2941 ttccgttcga gtgctttttc gataacgaac tcattttcag tagtcataga aatagttgct
     3001 aatttacgag gttcatcagc atttttttca ttaataacta cttcatgttc attttgttct
     3061 gtgtaaaatt taggatactc agcaacggct tttgaaaaat cagcaaaact taaagcggca
     3121 ttcgtatcag ttgagcgtgt attttcagtt gtgtccgctt ttggtttttc cgttttttct
     3181 tctggcacac tgactgtttc ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca
     3241 tcgtttttca atcgttgatc attccagtaa gacacgattt caataagaag attgtcacgg
     3301 aatcgttcga aaatcccgtt ttctcctgaa cgaattctct ttaatacagt attgaagttg
     3361 tcatcgattg gcacgttgat ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt
     3421 aagatttgac ggtttttggg atcaagcatg acttgtaacg gcaaaccata acgtcgcaag
     3481 ctctcttctt ctagagaacg gaataattgt cctcttacac catccaatga aggacggaat
     3541 tgatttaata atttttgttt ctgttttttc ttcatatgtt ctctccttcg ctcataccct
     3601 ttcgattata agaaaaaaac agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata
     3661 cgcaaattac aacagaaaaa tttgcgtatt ttcccgtttt ttgaaagaaa aataaaaaat
     3721 ggaacggatt atgtatatat acataatccg ttccattctt tctatttttt cggtatcaag
     3781 gcaaaaacag gtagttcagg tgaataaatg acttcatgtt cgtgccaggg tttgaccatt
     3841 agcgaatgat cgaaccgttt tttcagttct tcgatgattt gatcatgtgt ccagtttctt
     3901 gcaggaaaat gttggatttt ctctgcatcg atcaaaaaga caaaatcgga atgaaaagtc
     3961 tgggtaactt cttccgagag agaattcaag cgttcgattg gatccataaa atcacctttc
     4021 gacttaaaat tgggtagtat ctgggtcttt tgctttgccg actacaccat ctaactgatc
     4081 gatttgtttc atttcttctt ctgttaattc aaaatcaaat aattcagtat tttctttgat
     4141 tctgcttggt gtcactgact taggaagagg aaggaattcg tgttgcagcg accaacgcaa
     4201 agcaacttgt gcaatcgtct tgtcatgtgc ttcagcgatt tgtttcattt caggcacatc
     4261 gaagatttta ccagtgccta atggactata tgcttcgagg atcatatcat gtttttttgc
     4321 atactcaact acagttgtct gtaattcgcc tggtgcaagg aaaacttgat tgaccatcgg
     4381 cataattttt gctgtttttg ctagtgtatc tagatggtgg ggtaagaaat tgctcacacc
     4441 gattgctttg attttacctg cttcatacag ttcttcaaat gctcgccatg tatctgcatt
     4501 tgcttgttcc caattatcac gaaaagcaac agggtttggc cagtggatca aaaacagatc
     4561 taaataatct gtttggagct tcttcaatga ttcttcaaaa gaagacatca caagttcata
     4621 gctgtggttt gcattccata attttgttgt caagaatatc tcttctctag gaattccact
     4681 ttccttaatc gcttggccga cgctttcttc attcttgtat ccttgggcag tatcgatatg
     4741 gcgataacct gcagccagcg cttctttaac agaggaaaca gcgacatccc catcaggtgt
     4801 ttgccatgtg ccgaatccta ctaccgggat ttcgtaacca tttgatagac gataagtgct
     4861 tgttaatgaa ttcatacttt cagctccttt tttattcgtt caactataag tgtagcgcat
     4921 ttttcgaaaa aaagaatcca ttttgtttct actcttgaaa tttttgttta aaagaaaatg
     4981 attttgattc tggtattttt gatttttttt ccgtaaacta agacagaagg gaatgagaaa
     5041 atggatatct atttaaaaaa agcaattctg catattattg acagagaaac tggttctcca
     5101 gtcttttcac aaaaagagct ggatctgaca aaagaatata ttcgtgattt tcttcaaaag
     5161 aagatccaaa aaatctcttc tgctcaaaca aaaactggac aactagcaga agaaagcatg
     5221 atcgttgaat cacttaaaat cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt
     5281 cagcggtggt tcgatatcta ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt
     5341 gtcttgtatg aattagatac agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag
     5401 gcttacactc actttgttga agcagacgaa gcagggatcg acaaccgctt gattttgaat
     5461 cgagcaattc taggggcgaa aacacaaaaa gcagatgaag cattcgctgt gaatttgact
     5521 gattggacct acgaattaat tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat
     5581 ttttctggaa gagtgatcga aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg
     5641 atccaaaaag tagccaagca tcttggaaaa aaatttgaga cagaagaatt tgatatcatg
     5701 gctgatttga aagaagctgt ctatgatacg atagaagaaa aaggtcggtt ggatcatgag
     5761 atgattgcgg aaaaagtctt caaggaaaac atcacggcaa aaatggcttt taaagaagaa
     5821 gtacaagagc aaggatttgt cccagaagca cctcctgtaa aagaagtaca agaaatttct
     5881 gagaaaaaat ttggaaaaca aaaattcaag ctatcaaacg ggatcgaact gatcgtacca
     5941 gtagatgtat atcgcaaccc agatttgatt gaatttgtga ataatccgga tggtacgata
     6001 tccgtgatga tcaaaaatgt ggatgaagta ttgaatcgtt tataaaaatg actagaatat
     6061 taggtgttca agtaccagtg atcagttaaa tgaattgtgt caagagaagg atcggaaaag
     6121 acgaatataa caa
//
LOCUS       DADOOS010000143         6110 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.144, whole genome shotgun sequence.
ACCESSION   DADOOS010000143 DADOOS010000000
VERSION     DADOOS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.144"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(322..807)
                     /locus_tag="J8C37_002406"
     CDS             complement(322..807)
                     /locus_tag="J8C37_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305823.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(946..1161)
                     /locus_tag="J8C37_002407"
     CDS             complement(946..1161)
                     /locus_tag="J8C37_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAZ5305824.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1385..2185
                     /locus_tag="J8C37_002408"
     CDS             1385..2185
                     /locus_tag="J8C37_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAZ5305825.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2204..4072
                     /locus_tag="J8C37_002409"
     CDS             2204..4072
                     /locus_tag="J8C37_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAZ5305826.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4065..4556
                     /locus_tag="J8C37_002410"
     CDS             4065..4556
                     /locus_tag="J8C37_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAZ5305827.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4544..5098
                     /gene="srlA"
                     /locus_tag="J8C37_002411"
     CDS             4544..5098
                     /gene="srlA"
                     /locus_tag="J8C37_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAZ5305828.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5116..>6110
                     /locus_tag="J8C37_002412"
     CDS             5116..>6110
                     /locus_tag="J8C37_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAZ5305829.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2162 a          968 c         1223 g         1757 t
ORIGIN      
        1 tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa
       61 ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt
      121 aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa
      181 gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat
      241 ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta
      301 aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga
      361 tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg
      421 tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat
      481 atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt
      541 ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa
      601 cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat
      661 ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga
      721 ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa
      781 caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc
      841 agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa
      901 gggaaggttc atgaagataa taactttttt aataattata gctttctaat atctttcatc
      961 aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac
     1021 gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc
     1081 aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc
     1141 aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat
     1201 caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat
     1261 gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg
     1321 tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc
     1381 acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc
     1441 aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt
     1501 tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc
     1561 ttctaaaacg gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga
     1621 tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc
     1681 aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa
     1741 aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt
     1801 gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc
     1861 tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag
     1921 gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca
     1981 agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg
     2041 ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga
     2101 tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat
     2161 ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag
     2221 atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca
     2281 aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga
     2341 aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga
     2401 ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc
     2461 ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg
     2521 tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa
     2581 gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg
     2641 actaagaata ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc
     2701 ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt
     2761 tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat
     2821 ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg
     2881 gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga
     2941 actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac
     3001 aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat
     3061 gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat atcaagagat
     3121 gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt
     3181 attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat
     3241 acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac
     3301 gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc
     3361 cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat
     3421 attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct
     3481 aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc
     3541 ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc
     3601 aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga
     3661 aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta
     3721 tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt
     3781 taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc
     3841 agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt
     3901 atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga
     3961 tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca
     4021 agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc
     4081 tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac
     4141 taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta
     4201 aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc
     4261 aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac
     4321 ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg
     4381 aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg
     4441 caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc
     4501 ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa
     4561 atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat
     4621 gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt
     4681 tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg
     4741 ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat
     4801 ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg
     4861 tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg
     4921 gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt
     4981 gcttgtggga ctgctgatga actttattgg tggttggata actgatttta ctactggatt
     5041 tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag
     5101 aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga
     5161 ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga
     5221 ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat
     5281 gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg
     5341 actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca
     5401 ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg
     5461 ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca
     5521 tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag
     5581 cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat
     5641 caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc
     5701 gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt
     5761 atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg
     5821 ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg
     5881 atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt
     5941 gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca
     6001 aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc
     6061 atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DADOOS010000144         6108 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.145, whole genome shotgun sequence.
ACCESSION   DADOOS010000144 DADOOS010000000
VERSION     DADOOS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.145"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(182..1144)
                     /locus_tag="J8C37_002413"
     CDS             complement(182..1144)
                     /locus_tag="J8C37_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5305830.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="J8C37_002414"
     CDS             complement(1146..2585)
                     /locus_tag="J8C37_002414"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAZ5305831.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="J8C37_002415"
     CDS             2770..4716
                     /locus_tag="J8C37_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAZ5305832.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="J8C37_002416"
     CDS             4980..5852
                     /locus_tag="J8C37_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAZ5305833.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1945 a         1049 c         1077 g         2037 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaat
//
LOCUS       DADOOS010000145         6039 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.146, whole genome shotgun sequence.
ACCESSION   DADOOS010000145 DADOOS010000000
VERSION     DADOOS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.146"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..66)
                     /locus_tag="J8C37_002417"
     CDS             complement(<1..66)
                     /locus_tag="J8C37_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAZ5305834.1"
                     /translation="MVQIKTTTILIYLLAIQELSKR"
     gene            complement(81..1031)
                     /locus_tag="J8C37_002418"
     CDS             complement(81..1031)
                     /locus_tag="J8C37_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAZ5305835.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1028..1870)
                     /locus_tag="J8C37_002419"
     CDS             complement(1028..1870)
                     /locus_tag="J8C37_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAZ5305836.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(1872..2591)
                     /locus_tag="J8C37_002420"
     CDS             complement(1872..2591)
                     /locus_tag="J8C37_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305837.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            3283..3441
                     /locus_tag="J8C37_002421"
     CDS             3283..3441
                     /locus_tag="J8C37_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5305838.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            3513..4739
                     /locus_tag="J8C37_002422"
     CDS             3513..4739
                     /locus_tag="J8C37_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5305839.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(4827..5219)
                     /gene="rpsI"
                     /locus_tag="J8C37_002423"
     CDS             complement(4827..5219)
                     /gene="rpsI"
                     /locus_tag="J8C37_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAZ5305840.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5235..5678)
                     /gene="rplM"
                     /locus_tag="J8C37_002424"
     CDS             complement(5235..5678)
                     /gene="rplM"
                     /locus_tag="J8C37_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAZ5305841.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         2047 a         1059 c          902 g         2031 t
ORIGIN      
        1 tctttttgat aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg
       61 taccatacct accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat
      121 ttcatcatcg catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa
      181 atcgttgaat agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc
      241 acactgcttt ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt
      301 tttatttgat ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc
      361 acattatacg ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta
      421 tcattcggaa ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca
      481 atatacttat ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac
      541 tgtttttcaa tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga
      601 tcttccttcc ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct
      661 gcaaaatagt ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca
      721 tttcctccag tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt
      781 tgaacatctt caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg
      841 tgtaaatcaa ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta
      901 actactactt ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa
      961 catgccccga atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat
     1021 tttctcatca taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa
     1081 aactaattac aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa
     1141 aactataacc aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa
     1201 atatcatagt tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca
     1261 ttgcaacaat taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata
     1321 gaaccatcaa tagataatga taaaaactga cattcatccc tattgctttt gccattaatg
     1381 gatcaaaaga agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa
     1441 ccgaaacgcc aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata
     1501 aaatatgaaa taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta
     1561 aaaaagaact gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta
     1621 taaaagtaat aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa
     1681 tacctaaaat ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca
     1741 taagagacat tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa
     1801 ctacagatgt aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat
     1861 tttgaagcat attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa
     1921 ttttcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga
     1981 ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt
     2041 tttccctgat gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct
     2101 atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct
     2161 aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt
     2221 cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt
     2281 tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac ttttattgga
     2341 aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg
     2401 atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta
     2461 agcaatgtgg attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt
     2521 atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc
     2581 acttctaaca taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca
     2641 aaaattatat tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga
     2701 ttagaatgac aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa
     2761 aaaggagcgt tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa
     2821 ttatacttga agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa
     2881 ttattcaaga ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact
     2941 tttttcgtag tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa
     3001 ctatttttga tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc
     3061 tagaaactca agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt
     3121 ctgggaaaac aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc
     3181 gtaaactggg gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata
     3241 actactattt taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc
     3301 tgtgcatttc taggaatcga taataatact ctagataagt ggattcgtat atataatttc
     3361 ccagtaataa gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg
     3421 aatacattta aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata
     3481 tactgcctta ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat
     3541 ataaaactaa tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa
     3601 agtatcaaaa aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga
     3661 agaaaataga aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa
     3721 taacgtttaa aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa
     3781 ttagatcaaa caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt
     3841 tgcatatttt acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga
     3901 taatatcaga tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag
     3961 gatatgttcg ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa
     4021 ctactaaaat tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa
     4081 tacaagaagg taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa
     4141 atgatgattt taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc
     4201 tgtatcttgg tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg
     4261 aaaaacaaac ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa
     4321 aaggtcgaaa agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat
     4381 ggaaatcggt acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt
     4441 atctctttac ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc
     4501 taaactatcg aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac
     4561 acaaactccg tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa
     4621 tttcaaatgc tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag
     4681 atatagttga taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat
     4741 atgtgttctt aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg
     4801 ccagaaacgt tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct
     4861 ttcttaagac ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca
     4921 cgtttaagag ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga
     4981 cggatagctc ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat
     5041 gcgccttttg tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt
     5101 ggaatgtatt cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga
     5161 cgtacgcgag caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat
     5221 gaaattccct cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca
     5281 tgtggatgtt ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg
     5341 tttttaggaa gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc
     5401 aattcgccag cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt
     5461 ttgtcagtcg cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa
     5521 tcgccagtat ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca
     5581 acaactgttg aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt
     5641 tctacttcgc ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt
     5701 tctgatgttt gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt
     5761 taacatatta cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta
     5821 tatgaaactt tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa
     5881 aaacaaaaag aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt
     5941 tctcctgcca actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt
     6001 taaaattgtt tcttgtgact cggagctgaa cggcaggct
//
LOCUS       DADOOS010000146         6017 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.147, whole genome shotgun sequence.
ACCESSION   DADOOS010000146 DADOOS010000000
VERSION     DADOOS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.147"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            81..1088
                     /locus_tag="J8C37_002425"
     CDS             81..1088
                     /locus_tag="J8C37_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ5305842.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1100..2104
                     /gene="neuB"
                     /locus_tag="J8C37_002426"
     CDS             1100..2104
                     /gene="neuB"
                     /locus_tag="J8C37_002426"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAZ5305843.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2101..3249
                     /gene="neuC"
                     /locus_tag="J8C37_002427"
     CDS             2101..3249
                     /gene="neuC"
                     /locus_tag="J8C37_002427"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAZ5305844.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3222..3899
                     /locus_tag="J8C37_002428"
     CDS             3222..3899
                     /locus_tag="J8C37_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAZ5305845.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3889..5031
                     /locus_tag="J8C37_002429"
     CDS             3889..5031
                     /locus_tag="J8C37_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAZ5305846.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5044..>6017
                     /locus_tag="J8C37_002430"
     CDS             5044..>6017
                     /locus_tag="J8C37_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAZ5305847.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEV"
BASE COUNT         2240 a          761 c         1160 g         1856 t
ORIGIN      
        1 tgataattag tttagttata tattttttaa agattaagaa tcgaattata gatgactata
       61 attcatagga ggagatataa gtgtttaaag gaaaaacaat attagtaact ggcggaactg
      121 gttccattgg aagtgaaatt gttcgtcagc ttttaaaata tgaaccaaaa gctatcagaa
      181 tttatagtag aggtgaagaa aaacaatttt atatgcaaca agagctctca aatcacacta
      241 atttgagatt tttaattggt gatatcagag actctcaaag gattgattat gcatgtcgtg
      301 atgtggatat tatttttcat gctgccgcaa tgaaacacgt tccagcaagt gaatataatc
      361 ctatggaagc tgtaaaaaca aatgtgatgg gaactcaaaa tgtaatagat gcagcgatca
      421 aaaataatgt aaaacatttt gtaggtatca gtacagataa agtagtgagt cctactaaca
      481 ccatgggtgc gacaaagtta ttgagtgaaa aattaataat agctgccaat cagtataaag
      541 gaaaaacaga aacaatattt tcttgtgtaa ggtttggaaa tgtcatgggg tcaagtggat
      601 ctgttattcc tctttttagt aaacaaataa aaaacaataa agatattact ttgacagatg
      661 ttgatatgac acgatttatg atgtcaatac cggatgctgc aagattagtt ctgtctgctg
      721 ctatgaattc aaaatttgga gaaacatatg tcttaaaaat gccggcaatc aatattttaa
      781 ctttagctca atcattaata aaaatatata atgatacaca tgatgaaaaa tatagaggtg
      841 aaataaaagt tgtaggtatc aggccaggag aaaaattata tgaagagcta atgactacag
      901 acgaaattga acgctcatat gaaaatgata aaatgtatat cattccacca cactatcatg
      961 ctactgacga actgtacaaa gattatgaaa aaattaaaat cactgcatac tcttcagatg
     1021 caacagaatt aatgagtgga caggaattta ttagaatcgc aaaagagaat aatgtcgtga
     1081 cttggtaagg agttttatga tgaaatcaga agtatatgta attgcagaaa ttggtgtaaa
     1141 ccataatgga agtctagatt tagctttaga aagtattgat aaagctaaag aatgtggagc
     1201 taacgcggtt aaatttcaaa catttaaaac gaataagcta gtttccaaaa aggctccaat
     1261 ggcaaaatat cagaaggata atcttaaaga ggacagttct caatttgaaa tgctaaaaaa
     1321 attagaatta tctcaatctg attttattag aataaaagat tattgtatac aaaataaaat
     1381 tgattttttg tctactcctt ttgatgaaga aagtgcagac tttctaaaac aaattggtgt
     1441 acaaggattt aaaattggct caggagattt aacaaattta ccttttctta gaaaaataga
     1501 tatgtatggg ttgccagttt tattatctac aggtatggct gatattcatg aggtcagtga
     1561 ggctgtagat tgtttcatag atagtccagt tactatttta cattgtactt caaattatcc
     1621 ggctagttct gaagatatta atttattggc gattaacaca ttaaaagaaa tgttcgggat
     1681 acccgttggg tactccgatc attcattggg atatgatgtt ggtatttgtg ctgttgcgat
     1741 gggtgctaag gtaattgaga aacattttac cttggataag gaactacctg gtccagatca
     1801 taaagcttct ttagatcctc aggaatttgc agattttgta aagcatatta gaaacactga
     1861 aatattttta ggtgatggtg ttaagcgagc aatgccttct gaacttagta caaaagaagt
     1921 atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg ggagatattc ttacagagaa
     1981 atctcttaca ataaaacgtc ctggaaatgg aatagctcct aagtttttat atcaatatat
     2041 tggcaaaaaa gttaaaagac caataagtgc agatagtgtt ctgcttgagg aagatgttga
     2101 atgaaaaaaa tacttatagt aagtggaaca cgagctgatt atggaatata taaacctgtt
     2161 gctgagaatt tactacaaga taattgggat gtatcatttg ttgtttctgg aatgcattta
     2221 tcccataaat atggatttac taaagaattg atcttagctg acggatttag aattagtgga
     2281 gaagttcaaa gtttgttttt agagaacaat aagggaaaca tggctcggtc tgtggcatta
     2341 gagataaatg gtttcacaaa tatatttgaa caaactagcc ctgattttgt attattacta
     2401 ggggatagag gagaaatgtt ggcagctgcg atagcttgtt tgtatcttgg aatcaaaaca
     2461 gcacatattc acggaggcga agtgtctggt actgtagatg aatcaataag acatgccata
     2521 acgaaaatag catctgttca ttttgctgct acggaaaaaa gcaaagagcg tttaataaaa
     2581 atgggggaag attcttggag agtttttcca gtcggagctc ctagaataga tactatttta
     2641 aataatagat tacctagttt tagagatata aaagaaaaat atcatctaaa atttgaaaag
     2701 aaaaaatacg ttttgcaagt atttcatcca gttgtaaccg aactagaaaa tatagaaaaa
     2761 caaattttag ctttgatcaa ttcaatggaa caacaatctc aaccaattat ttgcatttta
     2821 cctaatagtg atgctggtag cgaaattatc agaaaaatgt accagaaata tttaaaaacg
     2881 gatattattt atattacgaa tttagagccg gaggaatacc tgactatatt aaaagagtgc
     2941 aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt caaaatacca
     3001 gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat tataaatgca
     3061 aatctaaatg aaaatgaaat tgaaactgct ttgaataaaa tacaagatcc tcagtttata
     3121 aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag aagaataaca
     3181 aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca aaaacaaata
     3241 gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga cacgcaaaag
     3301 tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt gatgataagc
     3361 aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat agctgggcgg
     3421 atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta gtaagaaaaa
     3481 agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt catccaagtg
     3541 ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg aatgctgtaa
     3601 ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc attgttgagc
     3661 atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta gcgggtggag
     3721 tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag atgaagaaaa
     3781 ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt ccctcacatt
     3841 ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt gaattctaga
     3901 attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc taaggctttt
     3961 aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga aagtattaca
     4021 aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc tgccatacat
     4081 cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc ttcatttact
     4141 tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt cattgactca
     4201 gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga agaagctgaa
     4261 agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca aatggctgat
     4321 tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga agatgcagcg
     4381 gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac aattgggatc
     4441 tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct agtgtctagt
     4501 gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga tcaagcaccg
     4561 tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc tgccggtatc
     4621 ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga aattttcaat
     4681 cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga agtggatggc
     4741 atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc tattgatcaa
     4801 gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc tttgtggaaa
     4861 ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga taataatctt
     4921 agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag taatatgaca
     4981 gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta ggtgtgatat
     5041 tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact tagactcatt
     5101 caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc gagacgaaag
     5161 gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc aaaaaatttg
     5221 tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt tattataaaa
     5281 gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa tgaatattat
     5341 gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc ttctacattt
     5401 ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa aaaaatttct
     5461 tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga ttatcgcaat
     5521 ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat tgaattacct
     5581 tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca gaaattatcc
     5641 aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga aactggtata
     5701 gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt aactataatt
     5761 ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc atggaatgaa
     5821 gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga taataattat
     5881 tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt cacgcttttg
     5941 gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat taacttaatt
     6001 aacgacattg aggtaaa
//
LOCUS       DADOOS010000147         6002 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.148, whole genome shotgun sequence.
ACCESSION   DADOOS010000147 DADOOS010000000
VERSION     DADOOS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.148"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..395
                     /locus_tag="J8C37_002431"
     CDS             <1..395
                     /locus_tag="J8C37_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305848.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="J8C37_002432"
     CDS             398..1228
                     /locus_tag="J8C37_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAZ5305849.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="J8C37_002433"
     CDS             1244..2095
                     /locus_tag="J8C37_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAZ5305850.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="J8C37_002434"
     CDS             2092..2451
                     /locus_tag="J8C37_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAZ5305851.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="J8C37_002435"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="J8C37_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="J8C37_002436"
     CDS             2624..2929
                     /locus_tag="J8C37_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305852.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="J8C37_002437"
     CDS             2929..3240
                     /locus_tag="J8C37_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305853.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="J8C37_002438"
     CDS             3407..3823
                     /locus_tag="J8C37_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAZ5305854.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="J8C37_002439"
     CDS             3823..4491
                     /locus_tag="J8C37_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAZ5305855.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="J8C37_002440"
     CDS             4488..4865
                     /locus_tag="J8C37_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305856.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="J8C37_002441"
     CDS             4862..5161
                     /locus_tag="J8C37_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305857.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="J8C37_002442"
     CDS             5403..5819
                     /locus_tag="J8C37_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAZ5305858.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DADOOS010000148         5990 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.149, whole genome shotgun sequence.
ACCESSION   DADOOS010000148 DADOOS010000000
VERSION     DADOOS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.149"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(444..1226)
                     /locus_tag="J8C37_002443"
     CDS             complement(444..1226)
                     /locus_tag="J8C37_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305859.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1291..2127)
                     /locus_tag="J8C37_002444"
     CDS             complement(1291..2127)
                     /locus_tag="J8C37_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5305860.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2145..3554)
                     /locus_tag="J8C37_002445"
     CDS             complement(2145..3554)
                     /locus_tag="J8C37_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAZ5305861.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3568..5391)
                     /locus_tag="J8C37_002446"
     CDS             complement(3568..5391)
                     /locus_tag="J8C37_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5305862.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5671..5898)
                     /locus_tag="J8C37_002447"
     CDS             complement(5671..5898)
                     /locus_tag="J8C37_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305863.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1858 a         1285 c          969 g         1878 t
ORIGIN      
        1 ataaaaagca aaaaaaaaaa gaaagaaact tattcttgct aataaactac gcgaaaggag
       61 tctaacgttg tttgatataa cttagactga tcgttaactt attttttata tttctacata
      121 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
      181 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      241 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      301 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      361 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      421 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      481 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      541 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      601 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      661 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      721 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      781 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      841 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      901 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      961 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
     1021 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
     1081 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1141 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1201 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1261 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1321 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1381 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1441 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1501 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1561 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1621 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1681 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1741 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1801 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1861 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1921 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1981 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     2041 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     2101 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2161 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2221 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2281 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2341 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2401 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2461 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2521 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2581 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2641 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2701 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2761 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2821 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2881 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2941 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     3001 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     3061 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3121 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3181 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3241 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3301 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3361 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3421 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3481 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3541 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3601 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3661 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3721 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3781 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3841 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3901 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3961 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     4021 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     4081 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4141 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4201 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4261 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4321 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4381 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4441 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4501 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4561 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4621 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4681 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4741 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4801 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4861 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4921 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4981 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     5041 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     5101 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5161 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5221 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5281 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5341 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5401 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5461 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5521 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5581 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5641 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5701 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5761 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5821 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5881 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5941 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca
//
LOCUS       DADOOS010000149         5904 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.151, whole genome shotgun sequence.
ACCESSION   DADOOS010000149 DADOOS010000000
VERSION     DADOOS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.151"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..986)
                     /locus_tag="J8C37_002448"
     CDS             complement(<1..986)
                     /locus_tag="J8C37_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAZ5305864.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="J8C37_002449"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="J8C37_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAZ5305865.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="J8C37_002450"
     CDS             1811..2965
                     /locus_tag="J8C37_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAZ5305866.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="J8C37_002451"
     CDS             3180..4148
                     /locus_tag="J8C37_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAZ5305867.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="J8C37_002452"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="J8C37_002452"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAZ5305868.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="J8C37_002453"
     CDS             5178..5786
                     /locus_tag="J8C37_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAZ5305869.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa ggca
//
LOCUS       DADOOS010000150         5831 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.152, whole genome shotgun sequence.
ACCESSION   DADOOS010000150 DADOOS010000000
VERSION     DADOOS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.152"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            175..1200
                     /locus_tag="J8C37_002454"
     CDS             175..1200
                     /locus_tag="J8C37_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAZ5305870.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1375..2619
                     /locus_tag="J8C37_002455"
     CDS             1375..2619
                     /locus_tag="J8C37_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAZ5305871.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2772..3719
                     /locus_tag="J8C37_002456"
     CDS             2772..3719
                     /locus_tag="J8C37_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5305872.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3853..4482
                     /gene="upp"
                     /locus_tag="J8C37_002457"
     CDS             3853..4482
                     /gene="upp"
                     /locus_tag="J8C37_002457"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAZ5305873.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4621..4863)
                     /locus_tag="J8C37_002458"
     CDS             complement(4621..4863)
                     /locus_tag="J8C37_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305874.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4984..5056
                     /locus_tag="J8C37_002459"
     tRNA            4984..5056
                     /locus_tag="J8C37_002459"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5017..5019,aa:Thr,seq:cgt)
     gene            complement(5175..5813)
                     /locus_tag="J8C37_002460"
     CDS             complement(5175..5813)
                     /locus_tag="J8C37_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAZ5305875.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         1965 a         1034 c         1213 g         1619 t
ORIGIN      
        1 tttatttgtt tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg
       61 gtttatcaac aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt
      121 gaggataaac tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa
      181 acgaaacgat atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa
      241 cttgttgctt ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac
      301 gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat
      361 gtcgcctctt ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag
      421 attgtgaaga acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact
      481 ttgccctcag ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa
      541 aaaacactag aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg
      601 tcgggaaaac caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt
      661 accgggatta ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta
      721 agtgatccaa cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc
      781 gaagcagtca tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca
      841 ccaaaagccc ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc
      901 agagatggcg attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt
      961 gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg
     1021 tataacgaca actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta
     1081 gatgaaccaa cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg
     1141 gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga
     1201 tcaattggtt attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat
     1261 cagtgaacga aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat
     1321 ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta
     1381 gattacaaaa cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa
     1441 gaacataatt tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca
     1501 caaggaagta tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt
     1561 gggtgtgaat ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc
     1621 ggagcgaaat ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat
     1681 ctcgctttgg tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat
     1741 ctaacacatg gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt
     1801 gttgatccga caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat
     1861 caaccaaaat tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa
     1921 ttcagagaaa tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca
     1981 ggattagtag cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact
     2041 accactcata aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca
     2101 ttagcaaaaa aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat
     2161 gtgattgcgg gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat
     2221 agtgaacaaa tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga
     2281 accagagtca tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta
     2341 ggtataaatg gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa
     2401 aactcgattc cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg
     2461 ccagcaatca ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt
     2521 gtcaaagcat tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta
     2581 cgcgaattaa ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa
     2641 acagaaaagt tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca
     2701 atatcggtga tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg
     2761 gaggaaaagt catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg
     2821 caaatgcgcc tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag
     2881 aagaagtaca agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg
     2941 atactggcga atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata
     3001 aattaccagt tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc
     3061 atgataaatt gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat
     3121 atagtcgatc accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc
     3181 aacagctgaa taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag
     3241 gagattcagt aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg
     3301 gcctcccaat atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag
     3361 attcctatca tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt
     3421 gggaccaata tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag
     3481 caaatataaa tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg
     3541 tattaagaga tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta
     3601 cacaagtacg cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga
     3661 caaaggcaac gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag
     3721 ctttgcttgt ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga
     3781 gtttgactag tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg
     3841 agagcttaaa aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta
     3901 actattattc gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc
     3961 gctatgttga tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa
     4021 acaccgatca ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca
     4081 atcttgcgcg caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa
     4141 gtgggacata tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc
     4201 aaacttccag aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca
     4261 ggcggatcag caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa
     4321 tttgtttgtt tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat
     4381 attgacatct atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct
     4441 ggactagggg atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt
     4501 gtgacacaac ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct
     4561 ttttcacagc tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat
     4621 tcaaatcttt tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa
     4681 gttcgcattt tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga
     4741 aatcgttcca gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa
     4801 tttttcacca atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt
     4861 cataaaacct gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa
     4921 actaattttc catcggactc tttacaatca gccaaataga gtatataatt tcttttggat
     4981 aaggccagct tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg
     5041 atccctatag ctggcattaa aagagaagac agatatatca aggattttta atccattgat
     5101 acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt
     5161 atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc
     5221 acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac
     5281 aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta
     5341 tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga
     5401 atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt
     5461 atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct
     5521 gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca
     5581 ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt
     5641 ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa
     5701 aaaatgctaa taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata
     5761 gtgcccaata tccctttttt aagttctatt ttttttccta aaatgttatt catgataata
     5821 actcctacta t
//
LOCUS       DADOOS010000151         5809 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.153, whole genome shotgun sequence.
ACCESSION   DADOOS010000151 DADOOS010000000
VERSION     DADOOS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.153"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            450..1604
                     /locus_tag="J8C37_002461"
     CDS             450..1604
                     /locus_tag="J8C37_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAZ5305876.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="J8C37_002462"
     CDS             1598..2020
                     /locus_tag="J8C37_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305877.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="J8C37_002463"
     CDS             3143..4075
                     /locus_tag="J8C37_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ5305878.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="J8C37_002464"
     CDS             4092..4607
                     /locus_tag="J8C37_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAZ5305879.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="J8C37_002465"
     CDS             4903..5184
                     /locus_tag="J8C37_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305880.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="J8C37_002466"
     CDS             5186..5602
                     /locus_tag="J8C37_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305881.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DADOOS010000152         5780 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.154, whole genome shotgun sequence.
ACCESSION   DADOOS010000152 DADOOS010000000
VERSION     DADOOS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.154"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(89..1219)
                     /locus_tag="J8C37_002467"
     CDS             complement(89..1219)
                     /locus_tag="J8C37_002467"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAZ5305882.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1867..3069)
                     /locus_tag="J8C37_002468"
     CDS             complement(1867..3069)
                     /locus_tag="J8C37_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5305883.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="J8C37_002469"
     CDS             complement(3129..3332)
                     /locus_tag="J8C37_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAZ5305884.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="J8C37_002470"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="J8C37_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4869..5681
                     /locus_tag="J8C37_002471"
     CDS             4869..5681
                     /locus_tag="J8C37_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5305885.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1709 a         1189 c          921 g         1961 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta
//
LOCUS       DADOOS010000153         5704 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.155, whole genome shotgun sequence.
ACCESSION   DADOOS010000153 DADOOS010000000
VERSION     DADOOS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.155"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(302..805)
                     /locus_tag="J8C37_002472"
     CDS             complement(302..805)
                     /locus_tag="J8C37_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAZ5305886.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1164..2189
                     /locus_tag="J8C37_002473"
     CDS             1164..2189
                     /locus_tag="J8C37_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAZ5305887.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2512..3390
                     /locus_tag="J8C37_002474"
     CDS             2512..3390
                     /locus_tag="J8C37_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305888.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3866..3982
                     /locus_tag="J8C37_002475"
     CDS             3866..3982
                     /locus_tag="J8C37_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAZ5305889.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4910..5248
                     /locus_tag="J8C37_002476"
     CDS             4910..5248
                     /locus_tag="J8C37_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305890.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5342..5572
                     /locus_tag="J8C37_002477"
     CDS             5342..5572
                     /locus_tag="J8C37_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305891.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1922 a          854 c         1052 g         1876 t
ORIGIN      
        1 acaagctttt tatagaaaaa taatatacct gagtagggta taaattaaga cggaattcct
       61 ttctagattg agatattgac taggaagaat gttaagattt taagccttta agctaaaaaa
      121 aaaccctttt tgttaaataa ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct
      181 gctgcaataa tatagtagat tagagatgaa aatagatttt tttaaaccaa tgactgaata
      241 aaaagaccgt catagataca cattggaatg tgtctattac ggtctttttt tacgaacgat
      301 tttattgaag acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga aataagaata
      361 aaatgtatcc caaaataatg gctagacggt ctaacttatt tgtcgataga tgtttgttta
      421 gaagttgata ggcccataaa cctatcagtc ctcctgttgt attagttatc aaatcagtga
      481 tgtccgctac gccaataccg aataggaatt gaatcaattc tgccaagaag cttacaagga
      541 aaataatccc taatttttta gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc
      601 caaaaggaaa aaagacaatc aagttataag tggtctcacg taataccgtc gtattttcaa
      661 aagaaaaagt agaaaaaggg acgagattga cattgctgta agaataagtg agaaattgtg
      721 gaagattaaa tgatagcttg aataaaatca accataatag aaagaataaa taaatcccta
      781 ataaaaattt tgatacatac cgcatagaca tctcctccat ttaaaaagta taccatcgaa
      841 aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac caacgtagtt atcgtacgaa
      901 aattagaagc ttttttttgt tgggaatccg gtttctttaa caaagctaag atagcgagtt
      961 gcttggaaaa gttgctatct tttttctgtc aaaacgactc ggaatgttcg ttttttgttc
     1021 taatttctcc aagttgttta ctttttgata ctaagtttat gaaaaatcaa gaaatagttg
     1081 ttatactagg taatgctgac aaacgggatt ggcatttcat cttggttcag ctttttttcc
     1141 aattactagg aaggagggat agattgttca agacttattt tcgtttcaat aaaatacaag
     1201 ataatccttt tcgtatcatg aatctgatgc tacttatctt tattgtatta tggttaggtt
     1261 acataaatca tgattttttg atttcaagtt tttcatcatt agggatcttt acttttttct
     1321 attatcaaaa cataccgatg aaatatttgc tgaaacgtat gtttctgatc ggttgcggac
     1381 tgttggttgc tttcgttttg gggatcttat cgacttatgt tttatggctt gaaccatttg
     1441 ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg attattccat atttctaaac
     1501 caggtggctt gttttttgcg atgctttctg ccatgggaac gagcatgcag ctgccaatcg
     1561 ctcaattacc gatcgtctca ctttattttt ttatgggtgt cgtttttgcc ctgattgcgg
     1621 cagtgattac aaaattactg gattcacggc ctgagcagac aattgaaaaa gcaactttaa
     1681 aagaaagatt tcacgaagag cctttagtga tcattgattc tgttttctac agcgctgcct
     1741 tatttctttc cgtttatgtc agtcacggct tgaatctgca taatccttat tggttgacct
     1801 tgtcatgtgc atcgattttg ctagcagaaa acttggatgc gatgaagcat cgacaagtcc
     1861 aatatctgat tggatcgatg ggtgggttat gtgtgtcggc attcttgtca tttgtgccgt
     1921 ttacgcagtt gcagaccatt tttctgatca cttttctata tggaatcgca caatttctag
     1981 ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc gatggcgttg atgctatcta
     2041 cacttattcg tggagcatac ttgatttctt tgatcgaata tcgtttttta gggatcgtca
     2101 taggaagttt catcggttta ggtgttgcgt gggtaatgac ggttggtttg caacattatt
     2161 taacggttgt tcgtcaaaaa ttagaataga aggacaaata tatgtgccaa aagaggttat
     2221 gacaagattt ttgtcacaac ctctaattca aggtgttcaa aagccagatt ctcagcatta
     2281 agtcaaattc acacaaaaat gtggaaagga actttcaaaa aaattttata tttattctat
     2341 caaaaaattt tgtttatgat ggtaactgaa taaactttcc tgagatggtc aatgctttcc
     2401 ttgaaagtct tacagatatt ctttcttcat agaaagttta agttttatag ttagtgtaca
     2461 ataaaagtat gtttatacca tatggtgtcc ggataatcag tcatttaaac aatgaggttc
     2521 aaagatatga aaaggggaaa tttgatgaag aaaataagca atgaaatcca aattctggca
     2581 gaaactttaa ctcttcccaa agtcgttata gagcaaatga ttgtacaagc aaaaaacatc
     2641 cttgatgagg tgtttgagca atatagtcat gaactaatgg atcctaataa agcaaaggcg
     2701 actgcaaaga aaatagacaa agtctatgaa gaggaacctt ttgcagcatt agctatttat
     2761 ctatatgcag cttctcaaag ttggaaaaaa atttatcaac cattgaagat tccaagaact
     2821 atctatttgg caacaatgaa tgcttttact agatttattc atgaacattt ccaagtaaca
     2881 ggaagctatc gctttgaccg tggtttttgg atttggcatt acactagtgg cttgatcttt
     2941 cggattggtg aattggagta cgaaatgaca tattttccga aagggtacac ggatctaaga
     3001 ttagaaggaa agcagtgcct ttctcttcat attccttccg atgcagattt atctcagaat
     3061 aagataagag atagttacga aagtgccatt cgtttttttg aactttattt tcctgattac
     3121 cattaccaag tgatgtacac agatacttgg ttactatcac ccaatcttac taaatggttg
     3181 aaaaaagagt caaaaatctg tctttttgca gccgattatc gattattatc agttgatgaa
     3241 caggacgata gtggagtccc ttggatcttt ggacgagtag atgcccaaat acatgattat
     3301 ccagaaagta cgagtttaca gcgacaagca aaagagcaat tgcttgcagg cgagcacatt
     3361 ggctctggct taggtattct tacttcataa gtttagtact tcgaaatagt ttaaaagagc
     3421 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     3481 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     3541 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttagtgttct
     3601 atcgaacaca cagtacgttc aataggtaaa ctagctataa caatagatat taaagttaac
     3661 ggacaagagg gcctttttta ggtagctaaa acgtaataat gaaaatttat ttccagagat
     3721 aggagtcaat cagctagtct gatcgactgt tttaacaagt tgactttcat tttaactttt
     3781 gataccattt aaaacacaaa tcgtaattgt ttcgatttgt gttttttagc atgactacaa
     3841 tcaaagaaga aagtgggtcg tccatatgga aaaaacagat atctcaagtg cataccgccg
     3901 cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg aagatcatca aagatgtgaa
     3961 acggaatagt gggaaaaggt aagaagaagg aaacttaatt aaaaaaatag ccgaaaaacg
     4021 tttttcggct attttcaata atcggttttt gtgtaaggtt tccgaaagaa ctgctgttga
     4081 ttttccattt ttaacatatg ataaattggt agaatctttc ttttcaaatt gataccattc
     4141 cctaattcca gttatatagt taactacaga accacaaagc acgtacgttt ataaaagtat
     4201 tatctgtagt tatttttcaa ttccctttca acctcttatt ttgaccagta gtattgatca
     4261 ctttaccttt taacaccttt gttcgttttt gaaacatttt tagtcaagaa tggaaacgta
     4321 actacataat caaaggtttt gcgaaatatt tcatgaaata tttcatgtag aagcgagcaa
     4381 tacggttgca aataaccatt atcgtttaat aagcagttac gaaagctccc aatatctcta
     4441 acgttcagct cactccattt cttttcttca aaaaaatcag tgattcgtat ctctttctat
     4501 ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa tatgcaaaaa aataaaaaag
     4561 cagttactat tgctagaata acatcaagga ataataagtt ttggttttca gataatagtc
     4621 gataagttgt gtattggaat tttagataca gtaataaagt atttttcatg aaattttgtg
     4681 agaatcacta ttacaagtat ctttaataaa attaatggtt aaggaggaac ttaaagtaga
     4741 atgtaaatga atagttgtag tcagtcgatt gtgatactag actcaaagtt gcaggaataa
     4801 gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga agggcttttt tttatacttt
     4861 gttgattgta gtgtttattt tccctctgtt tagccaagta acattttcta tgactcaacc
     4921 agtttcaagt gaggaacatg ataacctttt tcgtgctaca gaagagttta agaaatatca
     4981 ggaaaatatt gttccggaga ctccattagc tagtgagcca attactgata ttgatggaaa
     5041 ccaaatagga tggtatatac agtacgaagt tatggtggta aataatgact caatagtaaa
     5101 aaatacagta ggcttagagc taacaccgat tgaaaaggtg agttcattgt taacgtatat
     5161 ttatactgat gaaagtgacg agactgagat cattttaacg gattactcaa aaattgctac
     5221 tgataaaaag tatatattgt ggatgtagac actagtgaaa tactttatga tatagaaatt
     5281 gatgaaaatt tactagatgt agcacaagaa atcgatcatc aaaagcagga agctattcga
     5341 tatgctggta gaaaagggaa aagaaaatat ttattacgtg aatgtagcaa aagttagaga
     5401 agatgaaaat gagtggaaag aattcaagag tcgctactca attaactcga ctccgacatt
     5461 tacagtttac agagaaggat cgatagaaaa aactgttttt tggactaaag aaagtggaat
     5521 gtctttagct gaagtagaag agtttttgga ttatgttagt atgcaacagt aagggagttc
     5581 ttattatgaa aaattttgac acatattatg ttattgtttt ttcgatatct ttgctgttaa
     5641 tattactaac tttaatgcag tataaagagt atccagagta ttttatttta acttttgtat
     5701 attt
//
LOCUS       DADOOS010000154         5666 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.156, whole genome shotgun sequence.
ACCESSION   DADOOS010000154 DADOOS010000000
VERSION     DADOOS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.156"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(72..542)
                     /locus_tag="J8C37_002478"
     CDS             complement(72..542)
                     /locus_tag="J8C37_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAZ5305892.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="J8C37_002479"
     CDS             872..2512
                     /locus_tag="J8C37_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAZ5305893.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="J8C37_002480"
     CDS             2666..3988
                     /locus_tag="J8C37_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAZ5305894.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="J8C37_002481"
     CDS             complement(4056..4490)
                     /locus_tag="J8C37_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5305895.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5392)
                     /locus_tag="J8C37_002482"
                     /pseudo
     CDS             complement(4555..5392)
                     /locus_tag="J8C37_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1696 a         1129 c         1038 g         1803 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     4981 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5041 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5101 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5161 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5221 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5281 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5341 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5401 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5461 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5521 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5581 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5641 aagaaagagg tcgtgagcct gccgtt
//
LOCUS       DADOOS010000155         5614 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.157, whole genome shotgun sequence.
ACCESSION   DADOOS010000155 DADOOS010000000
VERSION     DADOOS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.157"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(59..2008)
                     /locus_tag="J8C37_002483"
     CDS             complement(59..2008)
                     /locus_tag="J8C37_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAZ5305896.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="J8C37_002484"
     CDS             complement(2050..3108)
                     /locus_tag="J8C37_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAZ5305897.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="J8C37_002485"
     CDS             complement(3227..3682)
                     /locus_tag="J8C37_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305898.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="J8C37_002486"
     CDS             complement(4266..5069)
                     /locus_tag="J8C37_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ5305899.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DADOOS010000156         5427 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.160, whole genome shotgun sequence.
ACCESSION   DADOOS010000156 DADOOS010000000
VERSION     DADOOS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.160"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..380)
                     /locus_tag="J8C37_002487"
     CDS             complement(<1..380)
                     /locus_tag="J8C37_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305900.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLA"
     gene            complement(831..1715)
                     /gene="rsmA"
                     /locus_tag="J8C37_002488"
     CDS             complement(831..1715)
                     /gene="rsmA"
                     /locus_tag="J8C37_002488"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAZ5305901.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            complement(1708..2277)
                     /gene="rnmV"
                     /locus_tag="J8C37_002489"
     CDS             complement(1708..2277)
                     /gene="rnmV"
                     /locus_tag="J8C37_002489"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAZ5305902.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(2270..3046)
                     /locus_tag="J8C37_002490"
     CDS             complement(2270..3046)
                     /locus_tag="J8C37_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAZ5305903.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            complement(3201..4523)
                     /locus_tag="J8C37_002491"
     CDS             complement(3201..4523)
                     /locus_tag="J8C37_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5305904.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            4698..5285
                     /locus_tag="J8C37_002492"
     CDS             4698..5285
                     /locus_tag="J8C37_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305905.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
BASE COUNT         1579 a         1158 c          938 g         1752 t
ORIGIN      
        1 tctgctaaag aatgaatcac accgatcaca aaattgaacc cttggaaacg ccaattagca
       61 aaagtaaact gtccattttt taaaacgata ctcggatgct tttgcaagta ccaagcccca
      121 atcaacgcaa acacaagatg tgacaatgct cgcaatgcga tgataaccgg cgttgttaaa
      181 aagaaaccaa atgtcgtacc tagcgcaaca gccactgcaa ctcctggtgt aaaaaacatc
      241 gcaataaata atggcacatg agacgccaaa gtaaaggaag ctggtggaat catgatttta
      301 ggcatgacca ttgggataat gacccccatc ccaatcaata aagcagaaat cgttagtttt
      361 cgaactgaat tttttctcat tttttctctc tcctaatatg tcttgacaca taaatgtcga
      421 cttagtataa ctcacttact aataactgtc aagacacata ttcttttcta aatccataca
      481 aaatgagtgt gagtatcaag tataagaatt ttttgatcaa gaaacaaaaa taggagatgt
      541 gacaaaattt gtgtcacatc tccttgttcg gcataatagt ggtcaaaagc ttgcatcaag
      601 taataagacc aactaactca aaaacatgag aagctgtttt ttgaaattgt tccttgtgac
      661 ttagagttaa acggcaggcc cacaacctct ttcttaaacg gtgttcaaaa gcttgcatct
      721 gcggtaacaa gcccaaacaa acgaaaaaca tgaggaacta tttttgtttg gctgttctta
      781 ttacttgatg caggacagct ttttcacaac ttctgtcaca acctcttttt ttactgcata
      841 ttttctgaca tcgcattgct taatgcggcg aattcttgta atgacaaagt ctccccacga
      901 cgttttggat cgattccagc tgtttccaag ctttttgcta accatgcttt tgtctggtca
      961 tctttgccat acgaatgctg caagttattc caaagcgttt ttcttctttg ctgaaatgcc
     1021 gcttttgtca atcggaaaaa tgctttttcg tctgtcactt cgactgccgg tatatctctt
     1081 cttgtcagtt tcaaaatcgc tgaatctaca tttggctgag ggacaaatac tgtttttggt
     1141 acgatgaatg ctaaactggc ttccatataa tattgaacag caatcgataa tgaaccataa
     1201 gctttcgttc ctggttcagc agagatacga tcagctactt ctttttgcat catgacgact
     1261 agttcatcca caaccaaatc tgattcaaga aaatgcatca tgatcggtgt cgtaatatag
     1321 taaggtaaat tcgctacgac ttttaacgga agctcctcat gaaatgtttc tctaacagct
     1381 gttgacaaat cggtctttaa aatatcttga tgtacaatag tcacattgtc atagggttgc
     1441 atcgtatcag ccaatacagg gatcaaacga tcatcgatct caaatgctac gacttgtttt
     1501 gcatgcttgg ctaattgttc cgtcaatgca ccgattcctg gtccaacttc gatcacgttc
     1561 gtatgttcat ctatttgcgc agtttctacg attttcctta aaatatttgg ttcagtcaga
     1621 aaattttgtc ctaaactttt tttaaatgaa aaaccatgtt tcaataaaat ctcttttgtt
     1681 ctagagggtg tcgcaatttc acgatattca ctcatgttgt tcctccacta ttttcattgc
     1741 ttgggcgagt tctttcggcg taatacgaaa catcttcaaa cgtttttcca attgtttgcc
     1801 gttcgtgtaa ccgatgcgca actcgtcacc tagtttttct cgtttttctt ttgcttgtgc
     1861 cccagctatt aaaccatagt ctaaaaggtc ttgtctagtg atttcagaaa cagccgtttg
     1921 ggaaatgtct ggcgtcacga cctttttcaa tgcttctaaa atagcagcat cacttgcatg
     1981 ctcgacacca aggcttccct ttttctttgg tttagcttgt gtgcgcggca aaaaagcatg
     2041 ttttgcctga ggaacagctt ccataatgat ttttcttatt ttttctcctg aaaaatcagg
     2101 atcggtaaaa acgatcactc cacgagtctc ttgggcatgt tcaatttgca tcaggatctc
     2161 ttcattgatt gcagacccta ttgtttctat cgtatctgca tcaactactt cccgcagcct
     2221 tcttgtatcg tcttttcctt caacgactat aatctcttgg atcttcatct tatccatgtt
     2281 cgcttagacc aaataatcgg tgcgcatttg tccatgtttg ggcagccact tcttctgtcg
     2341 gaagattccg aagttcagca atcttttcta ctgtataacg agtatagcct ggttcatttc
     2401 gctttcctct aaaaggaact ggtgccagat aaggggcatc tgtttccaca agtaatcgtt
     2461 cgattggtac atttgctgca gcctcttgga cgtctaatgc ttttttgaaa gtgacgacac
     2521 cgctaaatga aatatgcatc cctaagtcaa gaaatcgttt tgcccattca taatcaccac
     2581 tgaaactatg catgattcca ccgatatctc gaacatcttc ttcttttagg atttgatacg
     2641 tgtcttcaag tgcttctcta gtatgaatac tgattggtag gttcatttct tttgcgatag
     2701 caatctgtct gcgaaatact ttatcttgga cttctttcgg gtcttccatc caatgataat
     2761 ccaagccaat ttcccctaat gctacaacct ttggtaaagt caattgttct tgtaatttat
     2821 tttcgatttc tttcgtataa ctgccagcct ctgtcggatg ccagccgata atactgtata
     2881 tatttggata gtcatggctt aattgcaaag acttttcaat cgttggcgta tcgaagccca
     2941 cgaccgccat tttcgttacc cccatttctt gtgctcgctg aatcgtttct ggaatatctt
     3001 cattgaactg ttcagcattt aaatgggtat gagaatcaaa tatcatgttt atctctcctt
     3061 ttcattctta ctttagcaca taagcactac ttgcagaagt agcaaaaatc aaaatagaag
     3121 aacttgtttt attgaaatag aaaaagcacg aagatctaga ggacctagtt cgtacttttt
     3181 ctgtctgttg ttgaaatatt ttactcttct gagcgttttt tctttttttg ataatagact
     3241 gggataccta tcaaggtaac acctataccg atcaatgcta atttcgtttg cgtcatcatc
     3301 gtcataccta aaatgaaaat agcaccgaga atcgcaataa tcggaacgat tggataaagt
     3361 ggtactttat atggacgtgg caattccggc gctttttttc ttaaaataaa gacaccgata
     3421 aaaatcagta aactgaatag ccacatcaca aaaatcaaca tgtctgttaa aaagtcgaaa
     3481 gaaccaaggc tcatcataat acaagctact gctaactgga aaaccgcagg aacataagga
     3541 accgtaaact tctttgaaag ctttgtcagc tgtttactga aaggtagctc gtcttctaaa
     3601 gccatcgcat aaggcacgcg gatcccagtt agtgtataac cgttcaatgc accgtatacc
     3661 gagatcaaga tcccaatagt gaccagcttt ccaccgattc ctccaaaaat gacgtctgat
     3721 gcttcagaag ctgcattcaa attaccggca agatgatcaa tcggcaaagt tttcaaaaaa
     3781 acaaaattga tcaatagata aacgactgtt acaaaactca aaccaagaat aattgcttta
     3841 ggcaaatctt tttccggccg tttcatttcc cctgccattg caccgacacc taaccagccg
     3901 tcataagcaa acaacgttgc taacaacgca ctgctcaatc cttcgacaaa agtaacatct
     3961 tttccagctt cgattggaaa taactggaca gttccttgtc ctggggtcaa taatccccat
     4021 atgacaatca cagcaattgg tattaattta acaatcaacg tcactgattg tacagaagca
     4081 ccgacttttg tccctaacaa attcaaccct gtcacagaaa cagcggtgat cactgcaatc
     4141 aacaaaagat aatcggtaga taattgtaat aagttcgtca attgtgtcgc aaagataatc
     4201 gataatgcag caatattagc aggaaagtaa atcaagcttt gcgcccatcc taataaaaag
     4261 ctgggcaatt ttccatacgc agcttcaata tattttacag ctccaccagt ttccgggatt
     4321 gctgtagcta attcagccac tgtcaatcca gcacagatcg ttaagaaacc acccaaaagc
     4381 caagctagca atgtcaaact tgtagactga gtactagcag taacagcagc agttttgaag
     4441 aagacgcctg caccgatcac ggttcccata acggtagcta atgcgccaaa cagattgatt
     4501 tctcgtttta gtttgccttt cattcccatc ctctttcttc tttgaaattt ggtataattt
     4561 tcagtataac tgagaatatt ttaatttacc attctttttt ctccactttc agtgaaaaac
     4621 tccctcttaa gacggatgta tttctttttt atttttcgta tactaaacct accttttcca
     4681 cgaaggagga acagtttatg gattttaagc gttcttatcc gaaattagca gattatttat
     4741 ccaaccgggt cgatttttcc cgtaaagaac aacagcagtc ccattggcat tggttcacat
     4801 taattttatt tggattaatg actgttgcaa cgttatcaca aaaactaata ttgctgactt
     4861 tgttcatcgc aatcgttgca ttgatcaaag gtcctgtgat gatcttgtgg gggatgttgt
     4921 attccttttt ggttgggctt tttccgccta tcggtatttt gctttcagcg attttctttt
     4981 tattgaatat cggaacattt acaaagaact ggcggatgac cttagtcggg atctattttt
     5041 atttctatcc ttttggagtg atggcactta gtgaagtcat gcattgggat aatcattggt
     5101 ttatagctgg ctccttgctt ttagggttga tcttgttaca cgtgatgttg acaaagctct
     5161 atcaacatta tggtatcgga cgtacgattt tctggtatgt cttttcgatc ccatttgcac
     5221 tgcttactgc tctcttacca agccgtttga agacaaaaat aaaaggttac cataaaatca
     5281 agtgatagga tttttacaaa ataaagaaaa caatcatcga agtcaaagcc gatgattgtt
     5341 tttttatcta tattttcaat cacacatata agagcacaaa ctaattgaac ataaagtgat
     5401 agaacttata attaacacat caacgct
//
LOCUS       DADOOS010000157         5426 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.161, whole genome shotgun sequence.
ACCESSION   DADOOS010000157 DADOOS010000000
VERSION     DADOOS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.161"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(87..545)
                     /locus_tag="J8C37_002493"
     CDS             complement(87..545)
                     /locus_tag="J8C37_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAZ5305906.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(558..1883)
                     /locus_tag="J8C37_002494"
     CDS             complement(558..1883)
                     /locus_tag="J8C37_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAZ5305907.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1900..3312)
                     /locus_tag="J8C37_002495"
     CDS             complement(1900..3312)
                     /locus_tag="J8C37_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAZ5305908.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3329..4093)
                     /locus_tag="J8C37_002496"
     CDS             complement(3329..4093)
                     /locus_tag="J8C37_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAZ5305909.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4197..5072)
                     /locus_tag="J8C37_002497"
     CDS             complement(4197..5072)
                     /locus_tag="J8C37_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5305910.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5327..5411)
                     /locus_tag="J8C37_002498"
     tRNA            complement(5327..5411)
                     /locus_tag="J8C37_002498"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5375..5377),aa:Leu,seq:caa)
BASE COUNT         1655 a         1144 c          798 g         1829 t
ORIGIN      
        1 aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc ataaatatca
       61 ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt aatctttact
      121 tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac aatgatcacc
      181 gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag tgtcccttct
      241 tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag ttcaactgta
      301 tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt tatggcatgt
      361 cgggtaggaa atatattatc gattattcct ttaactggcg catggatata tccatcatga
      421 ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc ttttactgct
      481 gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt tttaaagaaa
      541 tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac tttgatcatt
      601 tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa tgtaaatgct
      661 tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt ctctactgca
      721 acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag tgtaattggc
      781 tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga ccgtagattc
      841 ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc attcaacaag
      901 gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt caatacttct
      961 tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc tcgtccatcc
     1021 atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat atcaggatat
     1081 aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt gatgactgac
     1141 atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc gacttgcatt
     1201 tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa ttctttatca
     1261 taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat ccaattctta
     1321 cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat atcacaggca
     1381 ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat cgactctgga
     1441 tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc gacaatttcc
     1501 aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca cgtttcttgt
     1561 cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc caatcctcca
     1621 acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg atcatccgtt
     1681 gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac aatcaagtac
     1741 atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa tggcaaccgt
     1801 ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc gcctccagct
     1861 atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta ataatttatt
     1921 gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat ttttttcagt
     1981 tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga ccatggtggg
     2041 atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga tattggcttt
     2101 tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac ttgctaaccc
     2161 taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt cacgtcctaa
     2221 tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca aggcaaaaaa
     2281 gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat tgatgagata
     2341 atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt aacagacaat
     2401 ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa gaaacatgaa
     2461 actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac cgcttcccat
     2521 gattcctttg accatctctt tattttcttt ataagctgct ttatagatag ctagacatat
     2581 agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc cagcctgagg
     2641 atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag gagatgtata
     2701 gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa aggggatcag
     2761 caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa cgagcagttt
     2821 tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa aaacagctgc
     2881 gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa aataagaaaa
     2941 aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta ctaaaccgga
     3001 aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta agcctttttt
     3061 caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct tatttttgac
     3121 aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa cggcatttcc
     3181 agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca tgatcaatcc
     3241 cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct gcacatttga
     3301 tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact aactcaatca
     3361 aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg acttggtggg
     3421 tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac gctgcgtgac
     3481 acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc aaatattctt
     3541 ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca tcgaaacaag
     3601 gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc cgaaccggca
     3661 aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa gtatgagtat
     3721 gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt tgaatctgag
     3781 cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga taataagata
     3841 aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt ggtttgccac
     3901 acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt agtttcacag
     3961 catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt cctcgctgat
     4021 aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc gaattaaaaa
     4081 tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa ccaaataatc
     4141 atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc cttattttaa
     4201 tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat ggcaagttga
     4261 aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt gctatgttta
     4321 aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata aatcaagcgt
     4381 ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc tttatcataa
     4441 acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc tactattttt
     4501 aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat agtgatgaat
     4561 cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc gaccacttct
     4621 actgctatat catattcatt tggataaagg aatctcattt ctggcaacat aattgctgtg
     4681 acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt caatgtaata
     4741 tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat cacttcttcc
     4801 gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg tttgatcttt
     4861 tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg cttactcact
     4921 ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc gtcatcgatc
     4981 aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc tcctctatat
     5041 gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa acaattttta
     5101 aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt gaatagtagc
     5161 accctacata atttacaaaa atagtttaac acataataaa agcgttatca atatcttttt
     5221 tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa
     5281 aaaaatatag caactctcta taagaaagtt gctatattga taattatacc ggcggccggg
     5341 gtcgaaccgg cacgtcctca acggacactg gattttgagt ccagcgcgtc tgccaattcc
     5401 gccacgccgg caaatttaaa aataat
//
LOCUS       DADOOS010000158         5426 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.162, whole genome shotgun sequence.
ACCESSION   DADOOS010000158 DADOOS010000000
VERSION     DADOOS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.162"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            152..1084
                     /locus_tag="J8C37_002499"
     CDS             152..1084
                     /locus_tag="J8C37_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305911.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1305..3068
                     /locus_tag="J8C37_002500"
     CDS             1305..3068
                     /locus_tag="J8C37_002500"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAZ5305912.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3152..3610
                     /locus_tag="J8C37_002501"
     CDS             3152..3610
                     /locus_tag="J8C37_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAZ5305913.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3719..4402
                     /locus_tag="J8C37_002502"
     CDS             3719..4402
                     /locus_tag="J8C37_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAZ5305914.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4549..5163
                     /locus_tag="J8C37_002503"
     CDS             4549..5163
                     /locus_tag="J8C37_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAZ5305915.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5179..5385
                     /locus_tag="J8C37_002504"
     CDS             5179..5385
                     /locus_tag="J8C37_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305916.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1940 a          850 c         1111 g         1525 t
ORIGIN      
        1 ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc cagaatttga
       61 ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct atgaaaataa
      121 aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga ctcatttttt
      181 taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag agatcgcaat
      241 cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg cagaaacgaa
      301 gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc tagacttaca
      361 agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata ccaatgagaa
      421 catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc tcaatgacct
      481 atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg atgcagaaaa
      541 ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag atggacaagt
      601 atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt acggaaaaat
      661 tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat ggaatgaaaa
      721 tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag cggggagaca
      781 ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt atcgaaccca
      841 atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat ttcgttcatt
      901 agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt gcgatgctgc
      961 tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct cattaaaagg
     1021 tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa aacatatttc
     1081 ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg tagaaggttt
     1141 ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct ctgaaatttt
     1201 tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa gtgtaacaaa
     1261 tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt acagtattac
     1321 atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt gaggaaatga
     1381 aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat gtaggtgggc
     1441 acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat gtatttgatt
     1501 caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat aagcttttaa
     1561 ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga tattccaacc
     1621 aacatgaaag taaacatgac tattttacag tggggcacac atctacatct atcagcttag
     1681 caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg gtagcattta
     1741 tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat ggtggtgttt
     1801 tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat gagaatcatg
     1861 gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc agcaataacc
     1921 tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat ctggaaacgg
     1981 tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt cacgtccata
     2041 cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat cattggcatg
     2101 taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa acatttaata
     2161 tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct attgccgcag
     2221 tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa catccagatc
     2281 attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct gctttggctt
     2341 ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg gcatatgatc
     2401 aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc ggtggcggaa
     2461 cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac ttatcaagta
     2521 tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct atgctagatt
     2581 gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat ggtgtagaac
     2641 atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta aataatggag
     2701 gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa acagtaaaag
     2761 cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc acagaaaatg
     2821 acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca ttagaagatg
     2881 gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat tcttcaatga
     2941 acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta gggcagcttt
     3001 atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt gtcttgaata
     3061 ttcaataatt cattatgctc accatccaga taattgttta tactagtatc tggatggttt
     3121 tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag aatatgtcaa
     3181 aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct tttcatttgg
     3241 ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga taaagactgg
     3301 tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa aagttggtca
     3361 attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa atataaatgt
     3421 gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag gagaagggga
     3481 tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg cttataaaga
     3541 agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat ttgaagtaat
     3601 tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc ctgaatagaa
     3661 aagggttaca tttttatatg aagctgtatt tttatataac attactggag gagatattgt
     3721 ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt tggaaaacga
     3781 gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag aaggcggagc
     3841 agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa tcgttgattt
     3901 accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta ttactgcgac
     3961 agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac tggatgcaac
     4021 ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga ttcacgaaca
     4081 cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag cgcaagaaga
     4141 tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac gaaagacgca
     4201 gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc cagtaattat
     4261 ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag gtgctttttc
     4321 agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat atgtcgaagc
     4381 cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca aaaaagaaaa
     4441 attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact tttcattttg
     4501 tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat gtttgaacgg
     4561 agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc agtcatccct
     4621 agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat cgcacccttc
     4681 agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat ccagagaaaa
     4741 caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc tgttatccat
     4801 atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct gcctaatgag
     4861 gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat ttcagtacca
     4921 gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt cgagatacgg
     4981 aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata tcatttaaat
     5041 gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa tgctgtaagc
     5101 cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc acgtcccgaa
     5161 taaaaaggag cactagttat gttaaaaata agctataaac catcgacaga ttcaaaagaa
     5221 atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt agaagttccg
     5281 ccattgcaag accattttgt cgttactact gttactttag atggtaagga aatagaaatg
     5341 ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt tattccgaaa
     5401 agtgaaaaaa catataaaaa aacaca
//
LOCUS       DADOOS010000159         5371 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.163, whole genome shotgun sequence.
ACCESSION   DADOOS010000159 DADOOS010000000
VERSION     DADOOS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.163"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(100..1911)
                     /locus_tag="J8C37_002505"
     CDS             complement(100..1911)
                     /locus_tag="J8C37_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAZ5305917.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(1908..2273)
                     /locus_tag="J8C37_002506"
     CDS             complement(1908..2273)
                     /locus_tag="J8C37_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAZ5305918.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2436..2831)
                     /locus_tag="J8C37_002507"
     CDS             complement(2436..2831)
                     /locus_tag="J8C37_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAZ5305919.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2849..3811)
                     /locus_tag="J8C37_002508"
     CDS             complement(2849..3811)
                     /locus_tag="J8C37_002508"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAZ5305920.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3811..4023)
                     /locus_tag="J8C37_002509"
     CDS             complement(3811..4023)
                     /locus_tag="J8C37_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAZ5305921.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4044..4742)
                     /locus_tag="J8C37_002510"
     CDS             complement(4044..4742)
                     /locus_tag="J8C37_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAZ5305922.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4762..5304)
                     /locus_tag="J8C37_002511"
     CDS             complement(4762..5304)
                     /locus_tag="J8C37_002511"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAZ5305923.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
BASE COUNT         1565 a         1202 c          873 g         1731 t
ORIGIN      
        1 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
       61 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
      121 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
      181 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
      241 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
      301 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
      361 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
      421 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
      481 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
      541 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
      601 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
      661 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
      721 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
      781 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
      841 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
      901 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
      961 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
     1021 caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
     1081 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
     1141 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
     1201 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
     1261 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
     1321 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
     1381 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
     1441 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
     1501 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
     1561 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
     1621 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
     1681 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
     1741 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
     1801 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
     1861 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
     1921 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
     1981 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
     2041 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
     2101 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
     2161 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
     2221 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
     2281 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
     2341 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
     2401 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
     2461 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
     2521 aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg
     2581 tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct
     2641 aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc
     2701 attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt
     2761 agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc
     2821 cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac
     2881 caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt
     2941 gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg
     3001 ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc
     3061 aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt
     3121 cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt
     3181 tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg
     3241 tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag
     3301 gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc
     3361 cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat
     3421 cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga
     3481 tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc
     3541 agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac
     3601 ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga
     3661 ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc
     3721 tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac
     3781 ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc
     3841 aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa
     3901 acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc
     3961 acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc
     4021 catctttaag cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa
     4081 atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa
     4141 tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc
     4201 ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac
     4261 acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga
     4321 aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc accaccagac
     4381 agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata
     4441 taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac
     4501 agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact
     4561 aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg
     4621 atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa
     4681 gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc
     4741 atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct
     4801 ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg
     4861 aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct
     4921 attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc
     4981 tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc
     5041 ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt
     5101 ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc
     5161 tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc
     5221 tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg
     5281 taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg
     5341 aaaaaaagaa aaatagcaat cgaactttca a
//
LOCUS       DADOOS010000160         5278 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.164, whole genome shotgun sequence.
ACCESSION   DADOOS010000160 DADOOS010000000
VERSION     DADOOS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.164"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(1283..1948)
                     /locus_tag="J8C37_002512"
     CDS             complement(1283..1948)
                     /locus_tag="J8C37_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5305924.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2074..4110
                     /locus_tag="J8C37_002513"
     CDS             2074..4110
                     /locus_tag="J8C37_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAZ5305925.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4114..4752
                     /locus_tag="J8C37_002514"
     CDS             4114..4752
                     /locus_tag="J8C37_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5305926.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4780..5208
                     /locus_tag="J8C37_002515"
     CDS             4780..5208
                     /locus_tag="J8C37_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305927.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1752 a          801 c          960 g         1765 t
ORIGIN      
        1 aatccaataa aaaatgattt agaatatcaa aataacaaaa aagaggacta cctgtattcg
       61 tcctcttttt tgaccagatt ttgaccagtc taatttacat tctaatatat tcatatgagc
      121 taattttttg attaaatggt aagtttttcc ttgtttgttt tagtttaaac tagtgtgtaa
      181 tttaaaaatc tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga
      241 taacatcaag gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag
      301 agccgagatt atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc
      361 ttggtgttcg tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg
      421 ttgataagga agataagata actgtgttat ataaactagg taactaactg taacaagcta
      481 attttaagag attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag
      541 gtaattgtca tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca
      601 gaatcagttg gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt
      661 cccactaaca agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat
      721 gctaccgaaa tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc
      781 cattactcaa agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata
      841 ggcgcttgaa caatccattt tgtattttat tgagtactct tggataaact accagcgatt
      901 atcatattta tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa
      961 atataaaaaa gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat
     1021 attacttact agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa
     1081 tgtccatcct tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat
     1141 actataagcc agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag
     1201 gctttttcaa ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc
     1261 tatctctacg ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg
     1321 cgtcgtcata gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga
     1381 gcaggcgaac gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt
     1441 tttctacctc tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata
     1501 cccaacgttc gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg
     1561 gtttcaaaaa aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa
     1621 aaagatagac caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg
     1681 gttgttttga tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta
     1741 ttgattcggt agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta
     1801 aaagtgacag catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt
     1861 tatcggaaac accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt
     1921 aagttcgtat tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga
     1981 tttattctac atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat
     2041 tttatcaaag aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt
     2101 ttactcacaa tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa
     2161 caggtactct atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga
     2221 ttgccacaac attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat
     2281 gtctcatcag aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta
     2341 gatacatctt ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg
     2401 gatgtcaaat cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc
     2461 attgaagaat cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa
     2521 aaagaagcct atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac
     2581 actgattatg ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt
     2641 tcaaatacag acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc
     2701 cttttgttat catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat
     2761 gacttttcat tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca
     2821 ttcataattg caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt
     2881 tatttgttcc agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct
     2941 cttttattag ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt
     3001 ctttttacat ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca
     3061 actatcgttt tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct
     3121 ggtgaggact atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt
     3181 tattccaatc gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac
     3241 ggaagtatat tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga
     3301 gggataaaaa taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg
     3361 ttgatccaaa taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga
     3421 catacagatt taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa
     3481 aaagaaccga aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct
     3541 atcccctgga tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat
     3601 ttggaaagaa atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct
     3661 ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag
     3721 ttatcatttc cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt
     3781 cattacttga tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc
     3841 atgttatgca tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt
     3901 tattcgctct ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg
     3961 acaagtgtaa tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt
     4021 ttctttagct tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa
     4081 agtcagttat tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt
     4141 gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct
     4201 ggggatcttg tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata
     4261 ttaggattga atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa
     4321 caattaactg ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa
     4381 gattttgggt tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt
     4441 tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat
     4501 ataaacaaga agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc
     4561 gcctgtgcga gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg
     4621 tcattggata aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa
     4681 ggtacgttag ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag
     4741 atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt
     4801 tgtgcactag ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat
     4861 tcagttcacg gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct
     4921 aaaggtaata ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt
     4981 aatcatagct ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt
     5041 cattctaacc actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac
     5101 tctattcgat cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat
     5161 agaactgaca cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt
     5221 ttatataaaa tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtt
//
LOCUS       DADOOS010000161         5203 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.165, whole genome shotgun sequence.
ACCESSION   DADOOS010000161 DADOOS010000000
VERSION     DADOOS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.165"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            110..1084
                     /locus_tag="J8C37_002516"
     CDS             110..1084
                     /locus_tag="J8C37_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ5305928.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1066..1527
                     /locus_tag="J8C37_002517"
     CDS             1066..1527
                     /locus_tag="J8C37_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAZ5305929.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1577..2041
                     /locus_tag="J8C37_002518"
     CDS             1577..2041
                     /locus_tag="J8C37_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305930.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2055..2804
                     /locus_tag="J8C37_002519"
     CDS             2055..2804
                     /locus_tag="J8C37_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5305931.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2804..3634
                     /locus_tag="J8C37_002520"
     CDS             2804..3634
                     /locus_tag="J8C37_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5305932.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3634..4281
                     /locus_tag="J8C37_002521"
     CDS             3634..4281
                     /locus_tag="J8C37_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ5305933.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4379..5155)
                     /locus_tag="J8C37_002522"
     CDS             complement(4379..5155)
                     /locus_tag="J8C37_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305934.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1773 a          707 c          939 g         1784 t
ORIGIN      
        1 aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga taataggtat
       61 tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa tgagacaact
      121 acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa ttgatcatgt
      181 atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat atctatttga
      241 taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact atgctatacc
      301 aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca caacaccaga
      361 aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt gtttatctaa
      421 tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg attggggtaa
      481 agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat ttaatatttt
      541 acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg ataagttaaa
      601 tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat ggggtaaaaa
      661 ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg gagaaattta
      721 ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg ctattcattc
      781 tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat tcatcttagt
      841 gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca ctaaaaaatt
      901 cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta tagatagtgt
      961 agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa tggtagaagc
     1021 gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc aaatgaaata
     1081 ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt gattacaaca
     1141 cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat gaatgacgcc
     1201 acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt tgaggagagt
     1261 atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac ggatttaatt
     1321 ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat tcaaattatt
     1381 gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc tggtaaatct
     1441 atagaagaaa tagattttaa ggaattactg aataactctc aaaaaagtat ggttcatatt
     1501 aattcgcttt taaaagatgt cagttaaggt ggcaatttct attctataaa ttttaaaaat
     1561 tgaaggagga aatagaatga ttaaattagt gcgtgtagac cacagattac ttcatggaca
     1621 agtaatattt tcttggacta aacagtatga tattaaccat attattgtgg ctgataacaa
     1681 agttccagga gatccaatgt ctgtgatggc tttgacaatt gctaaacctg ctgattgcaa
     1741 attagacatt gttaaaatta gtgatgttaa agggattatg gaaacatccc caaatgataa
     1801 ttacatgatt ttaattaaag gacctaagga agcttttgaa ttagttcaaa atttaccaga
     1861 aattaaagaa attaattttg gcggtgtgtc taagaaaagc gagtccaaac aatatggaaa
     1921 ggcagtcttt ttaactcctg aagaattaga agccacaaaa aaaattcttg atcaagaagt
     1981 gaaaatattt gtgcaacaag tcccatcttc aagtattgaa aatgtagatt ttagtaagta
     2041 aggggggaaa ataaatgtta ttaaaagcca ttttattagg tttagttggt gtggtagctg
     2101 ttattgactc caggttaatt ggtcgacaaa atattggtag accactaatt ttgagtacac
     2161 ttgccggact tgtgctaggt gacttgcata cagggattgt tcttggggca tctttagaat
     2221 taatttctat gggattcgtc tcaattgggg cggctggacc tcctaatatg caacttggta
     2281 gtattattgc cactgctttt gcaattttaa ctaatagtag tacagaaaca gccttaacaa
     2341 tagctatccc agtggctgta gctggtgaat ttatgagtat tatcatgcgc atgataattg
     2401 ctcaatttgg acatgctgca gataaagcca ttgaaaataa taaatttaga aaagctcaaa
     2461 ttattcatat ttattggtca tttattttta atgcttttgt ttactttatt ccaatctttt
     2521 taacagtata ttttggtgct gacgttgtgg cagatttagt agataaaatt ccagaaatga
     2581 ttacagacgc cttgactgtc tctggaaata tgctttccgc attagggttt gcaatgctat
     2641 taagcacaat gttgagtaaa aaattatatc cttactttat ttttggattc tttattgttg
     2701 cctattcagg tctttcacta attggcgtga caatttttgc agctctgatt gcctttgtaa
     2761 tggatcaagt taaatatgga aaaagagaag aggcgcatgg ttaatggaaa atagtacaga
     2821 aaaaaaacgt ttaatcacaa aaaaagatct taataagatg tgtcttcgtt catggacaat
     2881 catgtgttcg tggaattatg aaagacaaat gcatatggga tttatgtatg ggatggcgcc
     2941 aattttaaat aaagtttata aaaatgatga agagaaaaaa agagaagctt atactcgtca
     3001 tatggcattt tttaactgta cgcctcaaat gacttcattt attatgggac tttgtgcttc
     3061 tatggaagaa caaaatgctc aagcagaaga aggaaacttt aatactgaat ctatctccat
     3121 gattaaaacc agtttaatgg gtccttttgc tggaattggt gattcatttt tccaaggtac
     3181 tttaagaatt attacatttg ggattggatt gtcttttgct aagcaaggta gtattttagg
     3241 accgatttta gctgttggcc tttttgctat accttctatt ttagctgttt tttacggcgg
     3301 taaattagga tatgagtctg gcaataaata ccttactaaa ttgtatcaag aaggtcttat
     3361 ggatcgagta atgcaatttg cttctatcat tggtttggct gtagtaggtg gaatggtggc
     3421 tagcatggtg ccaattgtca cccctttaaa attcactaca gggggaacag aacttgtttt
     3481 acaagactta cttgattcca ttattcctgg attattacca cttgtatgta ctttgggaat
     3541 atataaattg attaagaaaa atgttagtac aaatatgctc ttattaggtt gtattttggc
     3601 agggtttgtt ttaagtataa taggtatctt ataatgatta aggctctaat ttttgatatg
     3661 gacggagttt taattgatag cgagattatg tatcaaaagt gggtatttaa ttttttaaaa
     3721 caagaaaatt taagctatcc tttgtattct tatcaaaaaa agattggaac tacagcaagt
     3781 atttttgatg atatagaaaa gtacaatgag ggatgtaaca gtttagaatt gaagggaaaa
     3841 tttactaaat attggaaatc ccaaaagatt gattacttgg atatttttcc taatatacta
     3901 attgaacagc ttgaatggtt aaaaaagaaa aaaataaaaa tggcaattgc ttcttccagt
     3961 ccaatggata ctataaatga agttgttacc acttgtaaaa tagagtcata ttttgattgt
     4021 ttaattagcg gaagagattt gccagaaagt aaacctaatc ctactatatt ccttaaagct
     4081 tcagaacagc tcagaattcc agttgaagaa tgtctggtga tagaagattc ttataatggt
     4141 ataaaagctg gtaaaagagc aaatatgaag gttcttgcaa ttaaagataa acgtttttct
     4201 caagatgtaa gtctagcaga tgatgttatt tacgatatta aatatctgcg acaagaagta
     4261 caggtgaaat ttggaatata aataagtttt ttgatgtatt tagagaatat tagtaaaaat
     4321 aaaaatcatg cccaaatcta atcaaaattg attgctttgg gtatgatttc ttttaatatc
     4381 aatctattac tttaggatct gaatcatttt tatgcaccgt atctaaagtg atattttctg
     4441 tgacttcaat aacataacta attctatcac aacgaactgt ggattgagaa taatgaaatg
     4501 gttgattatt tttatctttg ccaacctttt ctaaaataaa tagcggatct cctactatac
     4561 attgcaataa gtcggaaagc tcaggtgttg cagataaacc gtttatttcc ataatagagc
     4621 tcgtagcttt gacatcatag tcatcttcta gaagtttata taatgaagta tattggctac
     4681 ctttttcaaa taaagttgga aatttttctt ctgatgcgaa aaaattatca attcccatcg
     4741 gggcaccgtc ttcccacatt aaacgtttaa tattgtaaac tctatcagtt acattgattt
     4801 gtaatttatt agctaaaaac tcatcagcct ttataatttt agaatgtaaa acagtatatc
     4861 taattttatg tcctcgggta gaaagagaat cgctaaatcc atgaactcca gctaatttta
     4921 aactttcttt tttttgagtt acaaaggtcc ctttccccct taatcgttca actgttccac
     4981 tttctgctaa aagtttaaaa gcttttcgaa tggtaatctt acttactcca taaattttac
     5041 ataattcttc ttctgtaggt aattgctctc ctggaagata ttttttttca cgaatatcgt
     5101 tacgtataga agcttctact tgtaaatata atggtctatt atcttgaaaa ttcatagtac
     5161 actccctaaa aagttatact tcattatatc acaatttatt aaa
//
LOCUS       DADOOS010000162         5158 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.166, whole genome shotgun sequence.
ACCESSION   DADOOS010000162 DADOOS010000000
VERSION     DADOOS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.166"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..151
                     /locus_tag="J8C37_002523"
     CDS             <1..151
                     /locus_tag="J8C37_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5305935.1"
                     /translation="PKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNG
                     KFCLA"
     gene            complement(185..1414)
                     /gene="hflX"
                     /locus_tag="J8C37_002524"
     CDS             complement(185..1414)
                     /gene="hflX"
                     /locus_tag="J8C37_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAZ5305936.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1416..2333)
                     /gene="miaA"
                     /locus_tag="J8C37_002525"
     CDS             complement(1416..2333)
                     /gene="miaA"
                     /locus_tag="J8C37_002525"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAZ5305937.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2337..3074)
                     /locus_tag="J8C37_002526"
     CDS             complement(2337..3074)
                     /locus_tag="J8C37_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAZ5305938.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(3165..3692)
                     /locus_tag="J8C37_002527"
     CDS             complement(3165..3692)
                     /locus_tag="J8C37_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAZ5305939.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3872..4465
                     /gene="clpP"
                     /locus_tag="J8C37_002528"
     CDS             3872..4465
                     /gene="clpP"
                     /locus_tag="J8C37_002528"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAZ5305940.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4569..5054)
                     /locus_tag="J8C37_002529"
     CDS             complement(4569..5054)
                     /locus_tag="J8C37_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAZ5305941.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1392 a         1148 c          876 g         1742 t
ORIGIN      
        1 accaaaagaa atggaattca accaagtcaa tcaaggattc atctcatccg ttgcgtccaa
       61 aagaaaccat atccctagaa aatcactaaa ttaccaaaca ccattagaag tttttttgag
      121 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taatattgtt aaaaaaataa
      181 aattttattg gttttttctt aaccattttg aattggtttt tgcaaaccct ttaacttcat
      241 attcgtttgt ttcttccatg tacgcttcgt ccaaaaccat tgtttgtctt ctcaactcgc
      301 ttaatttctg tccttcgttg cttggcacag aaagtgtata aggggtcatt agttccatca
      361 gctgacgttt gattgcttcg atcaacgcca tcttaccttc tttcgtctga gtagaaatca
      421 atacatttgg gaatagcgta ggcgtgaaca tcgctgggtc gatctggtct gccttattgt
      481 aaacagtcag tatcggcatt tctttcatat tcagttcatc catcaatttt aagacagtct
      541 gctcctgtaa aatacgatct ggagaactag catcgactac atgtagcaaa agatccatat
      601 tttgactttc ttctaatgta gaatggaaag catcgatcag ttgcgtaggc aggtcttgga
      661 tgaacccgac ggtatccgtt acagtaatct caaatccttc agcaaaacgc catctcttag
      721 tcaatggatc taacgttgca aacaattgat ctttcgaata agtatccgct tgtgtcaata
      781 gattcaagat agttgacttc ccggcattcg tgtagccaat aagtccaatc tgaaaaatct
      841 cgctattttt tcttttttgg cgattgcgct ctcgatgagc ttctacttct ttcaattctt
      901 tcttaacagc aaaaatttta ttccgtatat gtcggcggtc tgtttctaac tttgtttcac
      961 ctggccctct tgttccgatc ccaccgccaa gacgagataa tgttttccct tgccctacta
     1021 aacgaggcag caggtattct aactgagcaa gttcaacctg cagcttccct tctttcgaac
     1081 gagcacgcat cgcaaaaata tctaagataa gctgcacgcg atcaattacc ggaagaccaa
     1141 cagcatcggt aatcagttgg ctctgtctcg gagttagctc atgattaaag atgatcaaat
     1201 ccgcttcatg cgcatccgct tgttgaacta attcttgtaa tttcccttta ccgataatcg
     1261 tttgccgatc cacttgtggt ctcttttgtg taattgtgaa tacgacttct cctgatgcgg
     1321 tattcgtcag ctgaactagc tcttccattg actctgaaaa gtaacgctgg tttgcttcgg
     1381 tttctacccc tacgatgatc actttttctt ccattttatt ccgcctccag ccattttttg
     1441 attgtttgct caatctgact tttttgattt ggctcactta taagatcaaa ccactgtgca
     1501 tccaaacggt tacgaaacca cgtcaactga cgtttcgcgt atcttctcga atgtagtttg
     1561 acttgttctg ttgcttcttc caatgtttct ttcccttcaa aataagggaa aaattcttta
     1621 tatccaatac cttgactggc ctggacttct ccaagctcat acaccttacg cgcttctgct
     1681 actaaacctt ctgctagcat caaatcgact ctttgattga ttcgttcata aagaatacta
     1741 cgcttcgtat ctaatccaat catgaggtaa tcatacaatt gctttggctc ttctttaggt
     1801 gccgtgatac tatgtcctgt cgtctctaaa acttccagag ctcttatcac tttgcgttga
     1861 ttgttccagt ggattttctc agcagctgcc gggtcttttt ctttcaactg attccataat
     1921 gcttgggaac caattgtttc agctaacgtt tcatactttt ttctaatctc aggattcgct
     1981 tcttctgtcg ctcctaactg ataatcatat agaagggatt ggatatagag acccgtacca
     2041 ccagctatga taggtaggtg ccctcggttc gcaatctcgg taatcaattg tcgacctgaa
     2101 tgctgaaaat cagcggctga gtaacgttga gtaggttcga tcacatcaat caaatgatgc
     2161 ggaacattat tcatctcttc ttctttgatt tttgctgtac cgatatccag atttttatag
     2221 atttgcaatg aatcaccact gatgatctct cctccaaata gttgtgcgag ttccacactt
     2281 agcgctgttt taccgactgc tgtcggaccg acgatcacta atactttttc catattttac
     2341 ctacttttcg ttgattgttt ccaccgaaga gctttctccg gaaaatcagt tattattcca
     2401 tctattttga gttcttgaca tttggccatt tcttcttcat cgttaatcgt ccatgcacga
     2461 attgcttttg gaaaatcagc tgcctggact ggatgttcga acaaccaagt attttttgga
     2521 tgtactcctt ctatataagg ggtttctgct gctagttgga tctttttatc agaagtactc
     2581 atcaataagt ccagctgtat ctccggttcc agtaatgcga ttttttccaa agaatccaaa
     2641 tgaaagctgc aatatgcatg ttgaaaaggc caatctctac tttttaatgt ttcggctaat
     2701 ttttcttcga taccgggata ttcatactga tcagttttaa tttcaatcat tagcatccct
     2761 cggtaatttt tttggaggag cagtcctaac acttctctca attcaggaac tttagctgct
     2821 ggaaattctt tgctgaattt gccccctgca ttcaattttt tgagttcttc caatgttttt
     2881 tctcgaatca gccctttacc gttagtcgtc cggtccactt cttcgtcgtg cataacgatc
     2941 aaataaccat ctttgcttag atggacatct agttcgatac catctgcacc tactcgtaca
     3001 gcttctgcaa atgcaggaag cgtattttct gggtgggtgc cgctgcttcc gcgatgggca
     3061 tagatttgta acatctttct tcactccgtt cagttctatt gtatcattaa gaaaaaaaga
     3121 aagcattacc acgctctcat gtgcaacggc aatgcttcaa aatatcagct ccaaacatca
     3181 tctttcgaga agaaaccaat cggctggcgc gtcatagtct tcaactgagc ttttttttca
     3241 ttgatcattt cagtcagact atccgtaaaa ttctctaaaa tcacgcgtcc gcctttataa
     3301 gcatatccac caatcttcat tgtttcatct ggagataaaa tcacttttcg atgggagata
     3361 ttcttaaata gctcgtctaa aatataattt ggcagataaa aactattttg cgctgaaaat
     3421 gattggagat acgcaaaatc gtcaatcgtg atataacaat catcaaatgc atcaaagctg
     3481 acatattctt ccatcagcgt tgtccgtttt tgcaagatct cgttgatctt tttatttaat
     3541 tgattcagcg tctcgatttc tttgactttc aattccaatt cacgtctacg ctgttcttta
     3601 ttttccaagt gacgcatttg cagtatcatg ccgatataga caactacagc acctgtcagc
     3661 actccgataa ggccagtaat accaactatc aatctgttgt tcctccttgt tttgtttctc
     3721 tgcttatcat accataaagc gggtttatgc gactaatttg cctaagtttt ttatttgacc
     3781 ttttttgacc aaaaatgtat actacatttt aggtattaga aactaaatta tggtacacta
     3841 tttctaaacg aaatcaagga ggaactctat tatgaattta attcctacag tcattgaaca
     3901 atcttcacga ggtgaaagag cttacgatat ctactcacgt ttattgaaag accgtatcat
     3961 catgttaagc ggccaagtaa cagatgatct tgctaattcg atcatcgccc aattgctttt
     4021 cttagacgct caagattctg aaaaagatat ctatctttat atcaattctc ctggtggttc
     4081 agttacagca ggtatggcta tttacgacac aatgaacttt gtcaaagcag atgtccaaac
     4141 aattgttatg ggtatggctg cttcaatggg aagcttcttg ctgacagctg gtacaaaagg
     4201 taaacgattt gctttaccaa atgctgagat catgatccat caacctcttg gcggcgctca
     4261 agggcaagca actgaaatcg aaattgcggc acgccatatt ttgcaaacac gcgaacgctt
     4321 aaacaaaatt ttagctgaac gcactggaca accgcttgaa gtgattgaaa aagatacaga
     4381 tcgtgacaac tatatgacag ctgaacaagc aaaagcatat ggattgatcg atgaagtcat
     4441 ggaaaacagt tcttcattaa attaatctgt ttcaaaggaa gcttttttaa gctctcttat
     4501 ctaaaccaaa taaaaaagga tggtcaactc aaactgacca tcctttttta ttttatggat
     4561 gctactgcct atctttgctc cttgaatttt ccttgcgcat gcatccgacc tatctcgaca
     4621 taatgctgga tgttttcttt gtttttttgt atttcttctt ttgtcaaagg acggatcact
     4681 ttagctgggc gaccaaaagc caagacatta ggaggaataa cttttccttc tgttactaat
     4741 gatccagcac caatcaaact attctcacca ataaccgcat gattcaatat cgtggtcccc
     4801 atcccgatca gcgccccttt ttcgattgta cagccatgta gcatgcactg atgtcctacc
     4861 gtcacattct ccgcaatatc tacaggtgca tcatgatcga catggatgat tgtaccatct
     4921 tggatattcg ttctttgtcc tatttttatc caattactgt ccccgcgtaa aacagcttga
     4981 taccagatcg tcacatcttc tgacaatgtc acatcaccaa tcactgtggc atttgatgct
     5041 ataaaccgtt ccatatttat ttttctcctt tcattttttg tagaaactaa tcctattgaa
     5101 taaaggtatc atagcatata tttatttcat aagaaaaaaa acaggtaaaa cctgctca
//
LOCUS       DADOOS010000163         5052 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.167, whole genome shotgun sequence.
ACCESSION   DADOOS010000163 DADOOS010000000
VERSION     DADOOS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.167"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            53..1221
                     /locus_tag="J8C37_002530"
                     /pseudo
     CDS             53..1221
                     /locus_tag="J8C37_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1347..2426)
                     /locus_tag="J8C37_002531"
     CDS             complement(1347..2426)
                     /locus_tag="J8C37_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAZ5305942.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2529..2969)
                     /locus_tag="J8C37_002532"
     CDS             complement(2529..2969)
                     /locus_tag="J8C37_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5305943.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3026..3436)
                     /locus_tag="J8C37_002533"
     CDS             complement(3026..3436)
                     /locus_tag="J8C37_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAZ5305944.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3616..4050)
                     /locus_tag="J8C37_002534"
     CDS             complement(3616..4050)
                     /locus_tag="J8C37_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAZ5305945.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4150..4998)
                     /locus_tag="J8C37_002535"
     CDS             complement(4150..4998)
                     /locus_tag="J8C37_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAZ5305946.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1838 a          973 c          848 g         1393 t
ORIGIN      
        1 atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata
       61 ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac
      121 agtccacaca gaggagatag aaacagccaa atggacagaa accaagtggg ccaacgtcaa
      181 tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa
      241 aggacaaaca gcgaccatca aagcaaaaat tgactattca ggagacttag cacctatact
      301 acaatcagct gttacatttg agaatgttga tcccgctttg tcgattggct atgacgagaa
      361 cactttgact atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga
      421 cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca
      481 tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcta tcgactcagg
      541 aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc
      601 gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga
      661 tacagaaact caaccagagc cagacaatga atcaaaacct gaaataaccc ctggagaaaa
      721 accagatgta gacccagaag aaaaaccaga tgtgactcca gaaccagata cggattctgg
      781 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
      841 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
      901 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
      961 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
     1021 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
     1081 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
     1141 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
     1201 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
     1261 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
     1321 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
     1381 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
     1441 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
     1501 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
     1561 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
     1621 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
     1681 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
     1741 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
     1801 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
     1861 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
     1921 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
     1981 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
     2041 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
     2101 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
     2161 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
     2221 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
     2281 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
     2341 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
     2401 taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa
     2461 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
     2521 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
     2581 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
     2641 ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt
     2701 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
     2761 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
     2821 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
     2881 ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat
     2941 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
     3001 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
     3061 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
     3121 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
     3181 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
     3241 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
     3301 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
     3361 atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag
     3421 aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca
     3481 caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat
     3541 gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata
     3601 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac
     3661 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
     3721 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
     3781 tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
     3841 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
     3901 cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
     3961 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
     4021 tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
     4081 ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta
     4141 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
     4201 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
     4261 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
     4321 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
     4381 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
     4441 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
     4501 aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
     4561 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
     4621 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
     4681 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac
     4741 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
     4801 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc
     4861 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
     4921 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
     4981 ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt
     5041 ataacttact tt
//
LOCUS       DADOOS010000164         5026 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.168, whole genome shotgun sequence.
ACCESSION   DADOOS010000164 DADOOS010000000
VERSION     DADOOS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.168"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(4..1029)
                     /locus_tag="J8C37_002536"
     CDS             complement(4..1029)
                     /locus_tag="J8C37_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5305947.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="J8C37_002537"
     CDS             complement(1274..2545)
                     /locus_tag="J8C37_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAZ5305948.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="J8C37_002538"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="J8C37_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="J8C37_002539"
     CDS             3788..4261
                     /locus_tag="J8C37_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ5305949.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="J8C37_002540"
     CDS             complement(4434..4988)
                     /locus_tag="J8C37_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ5305950.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1590 a          955 c          746 g         1735 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct tcttaaaaat attctgagtt aatgat
//
LOCUS       DADOOS010000165         4978 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.169, whole genome shotgun sequence.
ACCESSION   DADOOS010000165 DADOOS010000000
VERSION     DADOOS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.169"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(484..858)
                     /locus_tag="J8C37_002541"
     CDS             complement(484..858)
                     /locus_tag="J8C37_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAZ5305951.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(881..2281)
                     /locus_tag="J8C37_002542"
     CDS             complement(881..2281)
                     /locus_tag="J8C37_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAZ5305952.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2285..2695)
                     /locus_tag="J8C37_002543"
     CDS             complement(2285..2695)
                     /locus_tag="J8C37_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAZ5305953.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2713..3204)
                     /locus_tag="J8C37_002544"
     CDS             complement(2713..3204)
                     /locus_tag="J8C37_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAZ5305954.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3214..4005)
                     /locus_tag="J8C37_002545"
     CDS             complement(3214..4005)
                     /locus_tag="J8C37_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAZ5305955.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3998..4771)
                     /locus_tag="J8C37_002546"
     CDS             complement(3998..4771)
                     /locus_tag="J8C37_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5305956.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1573 a         1016 c          666 g         1723 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttactct aggagtgagc atttttcgaa aaatactaaa aatttttcaa
      121 aataaaaaaa ggcatacaga caataaactg tctatacgcc tcacagactc tattctatac
      181 atctaatttt ggctgtcctg caaaaaatat gtgttagcct ccatcgaaag atcaaacttt
      241 ggaaaacatc attataactt tgcttggtaa gagctttgct aaaacatgat atgacttgta
      301 tacaaaatgt ggagtgtatt ccaataaata ggaaggatta caaaatttga aaccaaataa
      361 gtaaaaaata ctgtcacttt gaattaattt gaaatagtaa attctcatta aactattgaa
      421 atgctgatac caaaaaatga agtacttttc gttcatacca acttagagca cttcgttatc
      481 tatttatttc ttcatttggc ttatctctat aatctcatga tttggtccgt agaaattaaa
      541 gtaacggatt ccattttccc agaacggtaa ttccataata tttttatcta ataattcata
      601 tcctaaatct tgaattgaat tgaatacctc atctatgtca tccgtatcta atgctagatg
      661 atcaatggct cctctacact cagtagtttt atcttcccat atttctatta tcgttccatt
      721 caactcgaaa aagccgactt tacttcccac attcatttct tcattaatca gactaaaccc
      781 taaggattca taaaagattt ttgaagctcc aaaatctttt gttggaattc caatatgttg
      841 taaaccattt aatttcactt acatcatccc ttcaattgtt ttaaaatcca cattcataat
      901 ttattgaata gtctagttta tctaccttga ttgctccctc cgtaacagtc atacttaatg
      961 ctgttccgac tttatcatac attctagtac ttaaagcaac attatcaata tataaaacag
     1021 ctatcgtatc atcaacaatt aactttagtt catattcttt gcctatttcc aaaaatagtg
     1081 gtcgttgcag ccccttatca aacatttgag gccaaggata gttcggagtt tttccgaaag
     1141 taagctgatt attaatcaat gaaatacgaa attcgtatcc ttcttgagta tcactatcta
     1201 caaataattt taaagcaaac tcctttgtta acgatgtaat agtcaaagtt gtttcaaata
     1261 gaaaataatc ttcgatttta ggaacaatta ctctttcttt ttttccaaat tctttctcta
     1321 acttaaaact tgaaaatgga gtgatttctt taaaattctc aattattttt tcaggcagtt
     1381 taacaccaag agttttgtct gtcttttgaa taatttgatg tggaacaagt gcaccgcccc
     1441 aatcccagtt attttgatcg ttctcccctt cttttgttgc cacccagcca gttagaaaac
     1501 ggccagtttc attagttccg actgttcttg ctgcataata ggcttttccg tcgaaagctt
     1561 cgtctactgg tgatttccaa tctgaaaata atcctgctcc gactcgatat ttagtctttt
     1621 tatcttcaca atactctgaa aaaagtaaat accacgttcc atcaatttca aatatatcag
     1681 gcatttcaat catgttaaat tcattattag cccagtagtc tcctttgaaa ttccaatctt
     1741 ttaaatcttc ggattcaaaa tagaccaatc tccccgtcgg ctttttctta cttgctggtt
     1801 ttctagctcc tagtatcaaa acgtattttc catgatcttg atcaaaaatt gcaaaaggat
     1861 cgcgccaatc attcttatca tatccttctt gaggaacaag ttttgctgct tcgccatcct
     1921 ttttccaatg aattaaatct ttagaacttg caatcattaa cacttctgaa gtttttccat
     1981 ccaacttagc tttgcgatta tgtccagtat acaaagctaa atactcattg attccttgga
     2041 caatacttcc agcgtaaatg tattgatcaa tgtcatcgtt gccaccacga tgaattactt
     2101 ccccgtattc ttcaaatttc acaaaatcag tggttgtaac tagtgaccac ccaaaaggat
     2161 cagttaaagg tttaggattt ctcgtatctc gttgatgaaa tagataaaaa gttccatctt
     2221 tataaaaagg catgcaatcc ccaaaccaaa aatctttagg cttgtaaaaa attttttgca
     2281 tttatcactc atcctcttcg ttagtatgtc tttgcatatc tgcaagacac gcaattgtat
     2341 tcatatttct tagattttcg atgatatcat cgcttttttt tccttgtaat ctcaaattga
     2401 cagcctcaat caaaattcct aaattcatgc caccaagaag ttgaaaccca ttttgaaata
     2461 cttcacttct tttagcaaat tcgttgaaag gtgttccacc aatcaagtca gcaaatacca
     2521 taacttcttg attttttact tgagaaacta ttttttcaat gttttcacgg aattcttcaa
     2581 tgcctgcatc tccaagtgtt accacatgac aatttaaatt atcgccaaaa aacattttta
     2641 atgtatctac aaatccaatg gcaatatttc catgagatgt aattataaac tccatccact
     2701 catcctctgt tcttaataaa taaaatcctt taaaaattct tttgaatctc taggagtttg
     2761 tatcactgtc acttcaactc catcactaat tagttcttta aaagcctctc tctcttcttc
     2821 agtaatattt atcgtcggac ggatgcgttc tgttccattc cttcttgata aatttcctac
     2881 gtttacactt gttattggta aaccgttatc aatcaaaaat ttaacatcga ttggagaaac
     2941 tgcaacaata agaacttttt gtccttcata tttaccagaa agaatatttt taactgctac
     3001 ttcccttgta attattgaag ttctaacatt aggtggtgca gcgaggcgaa ctacagcttt
     3061 ttttacgtca tcattggcta cttcattatt aataaccata attctgttag cactcaattg
     3121 atttgtccat tgtgttgcta cttgtccatg aatcattcga tcatcaatac gaacatgtat
     3181 aatacctttc attttttcat ccatattcaa cacctacttc aaaatattta acgctcttaa
     3241 cgcaatagaa actactaaga ttaatagaat tacataaact ggtttaacct ttcgtcctaa
     3301 caaataatac acaattgcta ctacacatgc aggtaataaa ccaggcataa cttgatcaaa
     3361 gatttcattt ccggtcattg ttaagtcgcc ttgtttaaat tcaaagccaa cattcacttt
     3421 tacaactgtg gcaatgagtg caccgaccac cattaaccct aaaattgata ctgcatcagt
     3481 aatagacttt aattttgttt caatttgagt tagtaggctc gcacccgatt ctttcccagc
     3541 aaggtataaa ggataagcag taatccttaa taaaattgat gccagtatcc acaaaataat
     3601 tccaacaaaa ctaccttcca atgccatatt agctgcaatt gctccaaaaa tagtccatgg
     3661 aataactaca aatacactat ctccaattcc cgctaaagga cccattagac ctgttttcag
     3721 agcacttact gcttccaaag acttattttt ttcttctttt tcaattccaa cattcatacc
     3781 aataatgtaa ggcgctgtat acggcgtagt gttaaaaaat tgtaagtgag ctaaaacgga
     3841 ttgtttcatt ccttcaggat catctttata tgtgtccttg ataaaaggaa gcatagaata
     3901 cgcataaccc aacccttcca ttttttcata atttaatgaa gttgcaccat tgagaatcca
     3961 ccggaaaatt acttgtttgt cgatttttgt cagtttctta ttcatcttcg tcaaaacctc
     4021 ctgttgcagc tggtgctact tgagtattat ttgatctatt tttatatacg attagtgcga
     4081 ttgcagcgcc aagtaacgaa atccctaaaa ttggtatttg gaaataaact gccaaaacga
     4141 aaccaactaa caaataggca aagtaatctt ttgtaggtaa atatttaagt agcattccta
     4201 tcccaacagc aggtaatagt ccccctgcta cctttaaacc accggtaacc cattcaggaa
     4261 tataattgat tgcacttccc actaacgttg ggcctaatat cacagttaac aaaacaggaa
     4321 ttccagatac aaagcttgta ttaattgatg ccaaatattg catccactct aatttttgat
     4381 aatcttcttt ttgtgcgaat ttttctgcct tttgttgaaa aaaaatgttt gttgaccact
     4441 ttagaacatc tagttgtaca attaacattg ctaccggtat tgctaaagtt attcctatag
     4501 aagcatcctg gttagttgag attgctaaat aagtaccgat caatgctgct gtttgataat
     4561 ccggtactga tgcaccacca aaactagtaa tacctagtgt cattaattgc aaagtcccac
     4621 cgatataaag tccagtttga atatctccca taataagtcc ggtcaagaaa cctgcaacga
     4681 caggttggga ggttccgatt ttgggccctt ccttatccca gttaatgaaa aatccataca
     4741 acacaactaa aaatgtttgc caaaacgcca ttttgttctc tcctttcttc ttatactttt
     4801 atataagcaa ttactgtgcc aactaacatt atccaataaa gcgggaaaat acagtaaata
     4861 ttaattaaat gagcgtctaa atatactttg gacacccttt cattttttta gacgctagta
     4921 tgctatttag aaaatagtct gctcaaaaat tgtatagatt aactcatact ctcctttg
//
LOCUS       DADOOS010000166         4920 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.171, whole genome shotgun sequence.
ACCESSION   DADOOS010000166 DADOOS010000000
VERSION     DADOOS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.171"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(26..466)
                     /locus_tag="J8C37_002547"
     CDS             complement(26..466)
                     /locus_tag="J8C37_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAZ5305957.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="J8C37_002548"
     CDS             626..1132
                     /locus_tag="J8C37_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ5305958.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="J8C37_002549"
     CDS             1300..3213
                     /locus_tag="J8C37_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAZ5305959.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="J8C37_002550"
     CDS             3218..4219
                     /locus_tag="J8C37_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAZ5305960.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="J8C37_002551"
     CDS             complement(4421..4627)
                     /locus_tag="J8C37_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAZ5305961.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1813 a          755 c          995 g         1357 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
//
LOCUS       DADOOS010000167         4892 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.172, whole genome shotgun sequence.
ACCESSION   DADOOS010000167 DADOOS010000000
VERSION     DADOOS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.172"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..258)
                     /locus_tag="J8C37_002552"
     CDS             complement(<1..258)
                     /locus_tag="J8C37_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305962.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="J8C37_002553"
     CDS             complement(274..924)
                     /locus_tag="J8C37_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305963.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="J8C37_002554"
     CDS             complement(935..2107)
                     /locus_tag="J8C37_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAZ5305964.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="J8C37_002555"
     CDS             complement(2126..2998)
                     /locus_tag="J8C37_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ5305965.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="J8C37_002556"
     CDS             complement(3020..3940)
                     /locus_tag="J8C37_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305966.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="J8C37_002557"
     CDS             complement(3940..4209)
                     /locus_tag="J8C37_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305967.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="J8C37_002558"
     CDS             complement(4206..4586)
                     /locus_tag="J8C37_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAZ5305968.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1297 a         1065 c          801 g         1729 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc ca
//
LOCUS       DADOOS010000168         4788 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.173, whole genome shotgun sequence.
ACCESSION   DADOOS010000168 DADOOS010000000
VERSION     DADOOS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.173"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            564..989
                     /locus_tag="J8C37_002559"
     CDS             564..989
                     /locus_tag="J8C37_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305969.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="J8C37_002560"
     CDS             2141..3118
                     /locus_tag="J8C37_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ5305970.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="J8C37_002561"
     CDS             3128..3625
                     /locus_tag="J8C37_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5305971.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="J8C37_002562"
     CDS             4001..4282
                     /locus_tag="J8C37_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305972.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="J8C37_002563"
     CDS             4284..4700
                     /locus_tag="J8C37_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305973.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DADOOS010000169         4640 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.174, whole genome shotgun sequence.
ACCESSION   DADOOS010000169 DADOOS010000000
VERSION     DADOOS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.174"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            63..1574
                     /gene="gadC"
                     /locus_tag="J8C37_002564"
     CDS             63..1574
                     /gene="gadC"
                     /locus_tag="J8C37_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAZ5305974.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1587..2987
                     /locus_tag="J8C37_002565"
     CDS             1587..2987
                     /locus_tag="J8C37_002565"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAZ5305975.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3152..4592)
                     /locus_tag="J8C37_002566"
                     /pseudo
     CDS             complement(3152..4592)
                     /locus_tag="J8C37_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1450 a          767 c          878 g         1545 t
ORIGIN      
        1 ataagcgatt ttccataacc ttggtaggaa tgacaaatta aataggaaag ggaagtaata
       61 atatgaataa aaaaaagtta tctctattcg gtttttttgc cctgacagct tccatggttt
      121 tgactgttta tgagtatccg acctttgcaa catcacaatt acatttggtt ttctttcttc
      181 ttttgggagg tattttatgg tttctaccag ttgctttatg tgctgcagaa atggcaacag
      241 ttgaaggatg gaaagatggt gggattttca gttgggttag tcaaacttta ggagaacgtt
      301 ttggttttgc agctattttt ttccaatggt ttcaaattac agtaggtttt attacaatga
      361 tctacttcat actaggagcg ctgtcgtatg taattaattt tccagagctt aatacagatc
      421 catttatcaa gtttattggt gtattagtca ttttctgggt actgactttt tcacagttgg
      481 gcgggaccaa tcgtaccgct aaaattgcta aagcaggatt tgtcattgga attgttattc
      541 cttcgatcat tctctttggt ttagcagcag cctactttat cggcggtaat ccgattcaaa
      601 tggctattaa tgcgaaagct tttattcctg acttttctca gctttcaact ttagtagtat
      661 ttgtatcttt tatattggct tatatgggcg tagaagcttc agcatcacat attaatgagc
      721 tggaaaatcc ccaaagaaac tacccattgg caatgttttt actagtgatt ttggccatcg
      781 ccttagatgc aatcggcggt ttctcagtag cagcggttat tccacaacat gacttgtctc
      841 tcagtgctgg tgtaatccaa acatttgaaa cgcttatttt acattttaac agtcatttac
      901 attggttggt aaaagtgatt gctctcatga ttgcctgtgg cgttatgggt gaagttagtt
      961 cttgggtagt tggtccatca cgagggatgt ttgcagctgc tcagcgaggt ctgttaccag
     1021 ttttcttccg gaaaactaat aaacaagacg ttccagttcc gttgattatg atacaaggaa
     1081 ttatcgttac tatttgggca gcagtcttaa cttttggtgg aggaggcaac aatttatcat
     1141 tcttagtagc aatctcctta actgttgtca tttatctcgt aggatattta ctatttttcc
     1201 taggttactt tgtattagtt tttaagaaaa aagacttaaa acgaacttac aatgtaccag
     1261 gaaaaactgt tggcaaaacg attatcggtg gaattggttt tattttatca gtgttcgcat
     1321 tgcttatctc ttttgtacca ccagcttcaa ttgcagctag tgagcaccga acataccaaa
     1381 tcatactact tattagcttt gtagtgactg ttcttttacc ttttatcatt tatgaagtac
     1441 atgataaaag tgctcacaca actattgagg aacctactca cgtgaaagct gaagacgtta
     1501 atcccgctgt ttatccagct gctagaggag agcaccacgt tacaaaaaat gatgaacata
     1561 ttttaaaaca ttaggaggaa ttcattatgt tatacggaaa agataatcaa gaagaaaaaa
     1621 actatttgga accaattttt ggctctgcaa gtgaggatgt tgacttgcct aaatataagt
     1681 taaacaaaga atccattgaa ccacgaattg cttatcaatt agtacaagac gagatgttgg
     1741 atgaaggaaa tgcgcgatta aacttagcta ctttttgtca aacgtatatg gaacctgaag
     1801 cagtgaaatt gatgacccaa acgttagaaa aaaatgcaat tgataaatca gaatacccac
     1861 gaacaacgga aattgaaaac cgctgtgtaa atatgattgc tgatttatgg catgctccaa
     1921 ataatgaaaa attcatggga acttcaacga tcggctcttc agaagcctgc atgctgggtg
     1981 gtatggccat gaaatttgct tggcgtaaac gtgctgaaaa attaggtctt gatattcaag
     2041 caaaaaaacc taacctggtg atctcttctg gttaccaagt ttgttgggaa aaattctgtg
     2101 tatattggga tgtggaactg agagaagtcc caatggatga aaaacatatg tcaattaatc
     2161 tagatactgt catggattat gtggatgagt acacaattgg tattgtaggt attatgggta
     2221 ttacttacac tggtcgttat gatgatatca agggtctgaa tgatttagtt gaagctcaca
     2281 ataaacaaac tgactataaa gtatacattc atgttgacgc tgcatcgggt ggcttttatg
     2341 caccatttac tgaacctgat ctagtttggg attttcaatt gaaaaatgtt atctcaatta
     2401 attcttcagg tcacaaatat ggtttggtat atccaggtgt gggttgggtc ttatggcgtg
     2461 accaacaata cttaccagaa gaattagtat ttaaagttag ttacttaggt ggagaaatgc
     2521 caactatggc tatcaacttc tctcatagtg cagcacaact aattgggcaa tactacaact
     2581 ttgtacgcta tggctttgat ggttatcgtg atattcacca aagaactcat gatgttgctg
     2641 tttatttagc caaagagatc gaaaaaactg gtatttttga aatcattaat gatggatcag
     2701 aattgccagt tgtgtgctat aagctgaaag aagatcccaa tcgcgaatgg acactatatg
     2761 atttatctga tcgtctgtta atgaagggat ggcaagtccc agcctaccca ttacctaaag
     2821 acttggatca attaattatt caacgcttag ttgttcgagc agactttgga atgaacatgg
     2881 ctggtgatta tgtacaagat atgaaccaag caattgaaga gttgaataaa gctcatattg
     2941 tttatcataa aaaacaggat gtgaaaacgt acggctttac tcactaaaat tcttcagaga
     3001 aatataaaag aagttaatta actaataaaa ggagttgtga ttaccacaca gctccttaaa
     3061 tctatttgaa taaatatatg atattcattt gttacactct caatcttagc tgttgttcta
     3121 ttcatcatta ctcttattgt aagtagttca gtcaaatttg cttgttcaat tgatcgataa
     3181 cagtatgaat tgtttgaaaa gtaatcggat atataattgt tttctcacta cctaagcctt
     3241 cttggtataa agaactatcg caaagtataa tccgctgagt acaaaagaaa gtgtcgtcta
     3301 ctatttctct aagttgttta caaatgatat tactaataga aaagtgttct cccaaaatgt
     3361 cttctaactg ttgtcgatag tataaatacc taaagtctga cggagctact atgaacactt
     3421 caattggaga tttccgttta tgctcattta aaagacttaa actaatggtt agtttacgaa
     3481 tagaattctc attccaacgc agaaataaat tgttttgttt tcgccattgt tcaagaattt
     3541 catgaacttc taagtacaaa gcgcgttgct cttcatttaa caagtaagtc ttatcaggtt
     3601 gaaatatctg aatatcagcc caaagtgagc ggataaacgg gagaagtgct ttatcaaaaa
     3661 gtatctgatt ttcttcagat atattcagag ataagataag tttttgactg agctcaatca
     3721 ccaaagggtt atctgctaca tggttttgat aaacaacgtt caagtctttt tgtaacaatt
     3781 caaagttatc gcatagataa tttctagagt tgaatagaaa gtagatataa aggcattcat
     3841 cttcgtcatg acataaagag aaagaaagtt gctctaatcc tttctgtatt agctgaaaca
     3901 agggagtatg tcgggcaact cttttttctt cctgactaaa atatattggg ttagtcttag
     3961 atctctgcaa accgatagcg atacctaata aaataaattg tttttcaact gtagggtaac
     4021 ttcttgaaaa aaaatgtttt tctacataat caatcaattg ttgaccgctt tgaataattg
     4081 atttatttag ctggatcaac tctgtccgat ttgtctcata gattatcgtt agtaacaaac
     4141 ttcgataatc cttttcagga atttcaaaat ggccatcagc atttaagtac tgcatttgtt
     4201 taaaaaaatt aatcaactca tttcgtaagt tgtaaacttt gcttaacgaa atgaaggtag
     4261 tatcagctat attactccaa ttgtgttccc ctgataaata atgtcctagt acattgaaaa
     4321 agtcagagcg ctgatagatg agttgagtga gtgccaacct cgaaaacgaa gagtcgaatg
     4381 cgactgtaaa gctacttcta gatttctcca ctgaaataat ttgctgatgg agtatgtcgg
     4441 taatgcattc caagtcgtta gtgacagaca gtgagtgggc aaacgttcaa ttgatctgct
     4501 atttcgcgaa tctgaatggt ccctttttcg tgtaattgct cacaaatgta aacttgacga
     4561 aaaatacttt tctccagata ggtttccaac atagtactcc tcctagtcat tttatacata
     4621 actattataa ctctaccaat
//
LOCUS       DADOOS010000170         4611 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.175, whole genome shotgun sequence.
ACCESSION   DADOOS010000170 DADOOS010000000
VERSION     DADOOS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.175"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(86..340)
                     /locus_tag="J8C37_002567"
     CDS             complement(86..340)
                     /locus_tag="J8C37_002567"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ5305976.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="J8C37_002568"
     CDS             complement(451..705)
                     /locus_tag="J8C37_002568"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5305977.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="J8C37_002569"
     CDS             complement(898..1173)
                     /locus_tag="J8C37_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAZ5305978.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="J8C37_002570"
     CDS             complement(1145..2098)
                     /locus_tag="J8C37_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ5305979.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="J8C37_002571"
     CDS             2710..4203
                     /locus_tag="J8C37_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAZ5305980.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="J8C37_002572"
     CDS             4338..>4611
                     /locus_tag="J8C37_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAZ5305981.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DADOOS010000171         4602 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.176, whole genome shotgun sequence.
ACCESSION   DADOOS010000171 DADOOS010000000
VERSION     DADOOS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.176"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1056
                     /locus_tag="J8C37_002573"
     CDS             <1..1056
                     /locus_tag="J8C37_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAZ5305982.1"
                     /translation="KYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQT
                     VPAYRRTNDTLEETVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTA
                     FNGRELHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESIT
                     IWEEILLDLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRV
                     DDRKEVCDDFKMVYQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPL
                     AIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGF
                     QQAEGELEQMLSQLIEN"
     gene            1190..3379
                     /locus_tag="J8C37_002574"
     CDS             1190..3379
                     /locus_tag="J8C37_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5305983.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3376..>4602
                     /locus_tag="J8C37_002575"
     CDS             3376..>4602
                     /locus_tag="J8C37_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAZ5305984.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1655 a          699 c          830 g         1418 t
ORIGIN      
        1 aagtatgatc gcattggttt taacacgggt aattctcgta acggctccta tgaccgtacg
       61 gtcaagaccg agtatgggga acttcatctc cagattccgc gcgaccgcaa tggtgagttc
      121 aagcaacaga ctgttcctgc ttatagacgg acgaatgaca cgttagagga gaccgtcatt
      181 cacctcttcc gaaaaggtat taccatgtcg gaaatcgcag acttgattga gaaaatgtat
      241 gggcattact acacgcccca aaccatgtcc aatataacaa aatcatttac agaagaggta
      301 acggcgttta atgggcggga gcttcatgac cgttatgctg ctatttatat ggacgcaacg
      361 tatattccgt taaagcggaa aaccgtcgcc aaggaagcta ttcatatcgc agttggcatt
      421 cgcccggacg gatcaaagga agtattgagc tatgcgattg caccgactga atccatcacg
      481 atttgggagg aaattttatt ggaccttcaa gagcgcggtt tgaaaaatgt cctcctgttc
      541 atcacggatg gcttaaaggg gatggtagga gcgattagtc ggttctatcc caaagctcgt
      601 tttcaacatt gttgtgtaca cgtttcccgt aatatcagtc ataaagtgcg tgtcgatgat
      661 cgtaaggaag tctgtgatga ttttaaaatg gtgtatcaag cctcatctaa agaggtggcg
      721 ttggaagcac gtggtacttt tgcggaaaaa tggaaaacca gctatccaaa agtggttgaa
      781 tcgattcttt cgaacgatca cttgctcact ttctatgatt tccccttggc catacgcaag
      841 agtatttatt ctacgaactt gattgaatcc tttaataaac aaatcaagaa atacagccac
      901 cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt tcttagtctc gtcttttgat
      961 acttacaacc aaaaattcct aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag
     1021 ctcgaacaaa tgctaagcca actgattgag aattagaaac aatctgcagt agggaagaat
     1081 catttacaca aaattattga cgctccctta taatgagaac ttttactaac ataatttatg
     1141 ataaacttac tatataatca tattaattaa atagttagga gatctgctta tgtttatatc
     1201 tactcttcaa attcgaaatt ttagaaatta tgaatccgaa atctttcgat tcgataatga
     1261 gacaaatact attataggtg agaatgattc agggaaaacc aatgctctta ctgcattgag
     1321 aatcttatta gatgatagtt attactatag tagtaaaaca ctaaaagaat cagatttttt
     1381 tcatggaatt caaaatgggt ggcaagggca ttggattatt atctctgcga catttgaagg
     1441 aatcagcgaa gaagaatttg acaatgagat ttgtgcttcc atttctttaa attctgaaag
     1501 tcaaacaatc ttagaagaat tgatttcaaa tgcagataaa ggtgtaggta gtatttctct
     1561 atttattaga cctaacaaag ctattagaaa gcaattattt gatatttcca ctgaattaga
     1621 ctctcatcaa tttaacgaat ttagaaattc tattcgtcta tccgactatg aattttacta
     1681 cacatctaaa tcaaatctaa atttctgtat caatgaaaat tatgataaat tggtaggaag
     1741 actcaatgac aatatagcta gtaatcctga agaagacgat gaagctctgt tgggctcaag
     1801 aattgacatg tcagatattt tcaagcatat ttcggttgtt tatgttgacg ctcttcgaga
     1861 tgttcttaga gaaatgaaaa acaatcgaaa cccagtcaaa agaatcatgg aaacaattga
     1921 atcaaaaata tcatcagata atgttgagtc attaaaaaca attatacagc aattaaatga
     1981 gactattacc agtgttcctg aaattaggaa aattggcgaa aatattaatc ggcaactaaa
     2041 tagcataatt ggttcagtgt attctcctaa tcttcttctt tcctcaacta tgtcagatga
     2101 tatgggttct ttagcaaaat ttctttcaat gaagccagaa caaaatatcg acttagactt
     2161 actagggtta ggacatctaa atatgattta tttagctttg aaaatagtcg aatttgaagc
     2221 gtgtcgttca agagaactac ttaatattat gttgattgaa gaaccagaag ctcatattca
     2281 tcatcatatt caaaaaactc tgttcgaagg attaaatctt caaaaaaact atactcagat
     2341 tctcatgacc acacattctg tacatttagc tgaagtgtct gaaatatctc ggatgaatgt
     2401 attaaaatcg tttaatggca aatctatcgt tatgaatcca gcaaataatc tgaactcatt
     2461 tgctaaagaa aagttaaaaa agaacaatct aaatctaata aacgcagtag agagatattt
     2521 agatgttaaa agaaatggat tgcttttttc taaaggtgtt atcttagttg aaggagatgc
     2581 tgaagaaata cttattcctc aaatagtaaa aaaattattt ggtataaact tagacgaact
     2641 tggaataggt ttgattaatg ttggtagtac ttcgtttgaa tatatcgcta gcttgtttga
     2701 cgatgataga atacagagaa agtgtgctat tgtaactgat ttagatgaac aggcaatccc
     2761 atcagaacat cgattgtata aaagcactgc acaagagaaa ggtcaagcaa gaaaagaaaa
     2821 attatcctcc ctttttaatg aaaacaattg ggttaattgt ttttatgcta atacaacatt
     2881 tgaaattgag tttttagatg tgaataaaga aaatattcta acttatgtca gtccaattat
     2941 agatgacgca ttcaaagata aaaaaacaat aactgcttat aaagaagaaa tacaaagtga
     3001 acaatattta gaaagaaatt tagcgatgtt aaaactagcc aatcatgttg gtaaggggtg
     3061 gtttgcaatt gagttaggtg aaaaaattga ctttttaatc aaaattccta agtatattct
     3121 taatgctatt gcttttgcat gtcaggaaat agtagatctc tcaatttata aaaaaattct
     3181 actccataca atcgacttgt acgaggaaac tacagaattg tcagctttat cagctagttt
     3241 aaaaacaagt gatacagctg atttgaatat agcaataatt tctttattag agtactttgg
     3301 taaagatgag gatgttgtgt atctaatcaa tcagatagaa aattacacaa aaatacttta
     3361 tgctgaggtt tcaaaatgac aaatatcttc gaaaaaatcg taaatgattt aaatttagaa
     3421 caaaaagatg ttgtggaaag tgaagataac ctttatgtaa atgcatgtcc tgggagtggg
     3481 aaaacaaggg tacttacaag gaaaattgct taccaagcaa ttaaacacga tggatcacta
     3541 aaaagaataa ttgctatcac atacactaat agagctgctg aagaaattaa ggaacgccta
     3601 agtttactta gtatagatga tgatattaat atttgggtgg ggacaataca tcagttttgt
     3661 cttgaattta ttatttatcc ttttaaaatg aatctctcaa gagtatcaaa aggatttaca
     3721 attattgatg attatactca aagagcttac gtcagccaaa ttaataatat gctgaattta
     3781 aatctcaaaa cgtatgaact gaacaaaatt gatttgtcac tttctcatga ttttaaaatt
     3841 aatgaagttc agtatactca agtttctgaa atgtatcaca ctatgttgga aaaaaataaa
     3901 gaaattgatt ttgatatgat tttactagta gcgtataaaa ttttaactgt aaacagtaac
     3961 gttgcacgta atattgctag taatataagg gcgatttatg tggatgaatt tcaagatact
     4021 agagaattac aatacaatgt aatagctaaa ttacttcaaa acaaccctca aattcagtca
     4081 atgtttgttg gagatattga ccaagcaatt tatggatcat tagatggtat agctaaatca
     4141 gttgaagaac tagaaatgct aactagtcac acttttcaat caaagacgct acatggctgt
     4201 tacaggtcta atcagagatt agtagacttc tactctaatt ttcagagttg tccttacgag
     4261 attgaaagca gagggaataa taaaaacgac cgtggtttta taagttatga ttgcaaagtt
     4321 actaaggagg atttacccaa cataataaaa aatataataa aggaaaagat tgaaagcggc
     4381 attcatgaaa aagatatttg tgtacttgct cctcaacact atcctctgtt ttcactgggt
     4441 gagtatttaa agaaagaatt accgtattgc aattttgatg ctataaatat atcaccaatt
     4501 aaggtaaata atcatagttt atttttcaag ttagcaattt tgtactttac tgaatcaggt
     4561 gaaaaggttc gaagaagaaa gctaattgca aacgatattt ta
//
LOCUS       DADOOS010000172         4574 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.177, whole genome shotgun sequence.
ACCESSION   DADOOS010000172 DADOOS010000000
VERSION     DADOOS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.177"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(22..3171)
                     /locus_tag="J8C37_002576"
     CDS             complement(22..3171)
                     /locus_tag="J8C37_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ5305985.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3185..4402)
                     /locus_tag="J8C37_002577"
     CDS             complement(3185..4402)
                     /locus_tag="J8C37_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAZ5305986.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4392..>4574)
                     /locus_tag="J8C37_002578"
     CDS             complement(4392..>4574)
                     /locus_tag="J8C37_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAZ5305987.1"
                     /translation="DTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPE
                     NEAWLQGALEVFKHEQ"
BASE COUNT         1227 a          974 c          758 g         1615 t
ORIGIN      
        1 aattttctta actcttctcg tttacctcaa ttcattttca agtggaatca actcatcatc
       61 taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggtt tcgcttctgg
      121 atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat
      181 tggatcttca cctggcatat attgcagatt agaagcatgt aattctttct cagaaacaaa
      241 ccactcattg gaaaaggcat gaatcaaatt atcacttgct tgtgtaaaat agcgacgttt
      301 aatgacaaag atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg
      361 tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc
      421 ttctcgtgca tctgaactat tcgcagcata agttcctagt aattgatcaa tataagtaga
      481 atcaatatca tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc
      541 tccctctttt tctggttctt cctccatcaa tgaacccttc acggtctcgt acacgccaat
      601 ttgttcttcc aaaagttgaa tttgattgtt caaagttgaa gattgcacca tgtcatcatt
      661 gtaatcatca taagtcacta acgattcata cgcacgattc aattcttgaa atgctttgac
      721 gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc
      781 ttttaactta ccatatgctt gcttgaatct ttttttggat tcttggtaac ttggataaat
      841 caaacccgat tcttctttag cttctgaata cagttttgtc gcatcctcga cattcttctc
      901 cattgtcgct ggctttcgga aagtaacaat tagtcctttt gttttttcag gatacgtacg
      961 gttcgtacgt gaaaaggctt ggatcaatcc tgcatactct agattacgat ccacaaatag
     1021 ggtttgaatc gtaggggcgt caaaacctgt caatagacga tccactacga ttactaaatc
     1081 gatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc
     1141 tccgttataa cgctcaatgt ctgctagtga ccaggcagta tcataatact gattatattc
     1201 ctcaataatt ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa
     1261 tgaataggta atagcgattc gtgggaaatc aggatcctcc atcgttcgtc cttcacggat
     1321 aggctgattg ggaaactctt ttattaacca atcagattct ttcgtcatct ctttgatggc
     1381 ccgataatac tttttggcca tatcaatgga actcgtggtt aagatcgcag acttcgttgg
     1441 acggccattt cgaaaatcaa attttgtata ggcattgtct ggacggaaca ttttatgaat
     1501 gactttttgg atatgttcct ctgcttcaaa aacagtaggg tccaaataac tttctttttt
     1561 gacagactcc atttgatcga tcatacgatt gatctgttcc ggtgaatact ctgcatatgt
     1621 ttctacttct tttaatttcc gatagatttt attctctaat gaagtcggct ccacggtgtt
     1681 ttcatgctct acttgaaatc ctaaaaccga gccatcttct aatgcattct tgatggtata
     1741 ggtgtgcaag acctctccat actgatcatg ggtcgtacgt gccaattgcc ctttggcttg
     1801 tttccgattc tcagggaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa
     1861 catttttttg atttcttcca tattttcagc acttaacgct cgatgacact catcaactac
     1921 aaacacgata tgttgcccca ttaatttttg gaagcgattg gttccttttt tctcttcctg
     1981 tttctttgca tatttcaacg ccgcatctag tttttgacgg gttgtaatga tcacaacatt
     2041 cgcattggca tctgccaaca gagtttcact taactctttg gcacttcctg ttcccacgat
     2101 caagctattc gctttggcat taccagaaga aaccccagta ttaaactccg aagcaaattt
     2161 cgtaaactct gtggtggtct gattatctaa atctttgcgg tccactaaca tgatcgttcg
     2221 atcgatcccc gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc
     2281 tgaaccagtc gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc
     2341 ttgaatcgca tgcacttgat aaggatgtaa caccatgagg gttttattgt cttgatcttc
     2401 actgacaatc gtataatccg caatcaagcg atgggcatct gggatattta gcacttgttt
     2461 gcaaaactca tataagtttt ctaccttttt attatctttc gttcgccaac taaataaaaa
     2521 cttaggatgc aaatccttcg gtaaggcatt tgcaaaatac cgagtcgttt gttcattgga
     2581 aaccacaaac aactgtaagg tcgaaaagat attgttccga aacatgcctt cttcagcata
     2641 ttttttgatt tgattaaacg cctgataaaa accatctttt gcagttactt gttttagctc
     2701 gatttggata atcggtaagc cattgatcaa taaggtgacg tcaaaacgtc gatcccgcgc
     2761 ttctatgtta cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc
     2821 accaatgtct tgattggaat aaaggataag tgagacagaa cccaattgcg gattttcccg
     2881 ttcaatcgtg atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg
     2941 ggttttcgtc cgtagcaata attcggtttt gatcgtttca aactctttat cggtcaaagg
     3001 agaatcattc agttctgctt gattatttga gatgattttt tgacgtaaat tgctccaaag
     3061 atcttcttct gattttagat ccggacgata cgtccactga ttatgtcctt ccccgaggac
     3121 acgaatcaac tgttcttcga cttccgcttc ctcacggtga ctaattttca ttcttcttcc
     3181 ctccctaaac aaacatcttt tgcaagaagc cttttttcgt ttccttcagc aaatctaact
     3241 tacgttgatg aagagcgatc gtatcatcta gctgcttgaa gaaggcacca attttttgtt
     3301 gttcatttat atccggtgga aactgaaatg tcctttgttc tataccacct ttagtcacat
     3361 gtaccattgt cgttccgtta gttgattctt taattttctc tttatcagtc aatagccaag
     3421 tatatagata ctgtttgtcc aattccttag ataaaaattt aagtttccaa atatgataat
     3481 ggtagattac acgttcttca ttccacattt caggaccgaa gcttgttgcc cataaatata
     3541 agagatctcc tttattagca tacttgtctt cctctaattc taagtttgaa taataccatc
     3601 tatcattagt attaaaatta cctactctta aaaccctata ttttccagaa tcaagtaatt
     3661 cttgttgttt aaaagctcta ccattcaaaa aaactaccac ttcccccaac ttccgctctt
     3721 cccaatctcc cgtaaatcct ggaaaacgaa cttctggcac ttttgctccg tttttaggga
     3781 acattttttg taagaatcct tttttcgttt ccttcagcaa atctaattcg tgctggtgaa
     3841 gcatgatggt gtgatcaagt tgtttgaaaa atgaaccgat cttgatctgt tcgtcataag
     3901 ttggaagtga gataggtgtc tttttaccac tatcaattgt ataggttccg acggttccaa
     3961 tgcctgcaac gtcacggata tacttctgaa ttttgttatt tatgaacgag tagtaaaaga
     4021 agtttccgtc aattttatgc tcattcttga accaaatgat gtttccatcc ttaaagtaaa
     4081 ttgggttatc atcagtgatt aataagggga cacctattga tccgacacca gttacaagca
     4141 agtctccttg agaaacttta cctgaatttt tcgaatattc attatatttt tcttctgaaa
     4201 tgtaaagata atcggatggt tcctcatttt ttgcagctga gacaatatca cgagcacgta
     4261 aaaatctaat acctgagtta gtccaatctg attgatgaat tcgcttcaca gaagtaacat
     4321 ccatcatatc tcctaactta cgctcttccc aatcttccgt aaaaccagga aaccggattt
     4381 ctggtcgtgt atcattgctc atgtttgaac acctccagcg caccttgtaa ccacgcttca
     4441 ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa gttctttttc tagcacttgc
     4501 tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa ccatatcaat tggttcttct
     4561 tcttcgaaag tatc
//
LOCUS       DADOOS010000173         4475 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.178, whole genome shotgun sequence.
ACCESSION   DADOOS010000173 DADOOS010000000
VERSION     DADOOS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.178"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            323..1054
                     /locus_tag="J8C37_002579"
     CDS             323..1054
                     /locus_tag="J8C37_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAZ5305988.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="J8C37_002580"
     CDS             1199..3226
                     /locus_tag="J8C37_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAZ5305989.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="J8C37_002581"
     CDS             3334..4431
                     /locus_tag="J8C37_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAZ5305990.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DADOOS010000174         4425 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.180, whole genome shotgun sequence.
ACCESSION   DADOOS010000174 DADOOS010000000
VERSION     DADOOS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.180"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            690..1658
                     /locus_tag="J8C37_002582"
     CDS             690..1658
                     /locus_tag="J8C37_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ5305991.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="J8C37_002583"
     CDS             1669..2484
                     /locus_tag="J8C37_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAZ5305992.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="J8C37_002584"
     CDS             2552..3262
                     /locus_tag="J8C37_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5305993.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="J8C37_002585"
     CDS             3298..>4425
                     /locus_tag="J8C37_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAZ5305994.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DADOOS010000175         4405 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.181, whole genome shotgun sequence.
ACCESSION   DADOOS010000175 DADOOS010000000
VERSION     DADOOS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.181"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            475..1386
                     /locus_tag="J8C37_002586"
     CDS             475..1386
                     /locus_tag="J8C37_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305995.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1873..2139
                     /locus_tag="J8C37_002587"
     CDS             1873..2139
                     /locus_tag="J8C37_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305996.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2211..2552
                     /locus_tag="J8C37_002588"
     CDS             2211..2552
                     /locus_tag="J8C37_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305997.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2607..3026
                     /gene="ssb"
                     /locus_tag="J8C37_002589"
     CDS             2607..3026
                     /gene="ssb"
                     /locus_tag="J8C37_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAZ5305998.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3103..3399
                     /locus_tag="J8C37_002590"
     CDS             3103..3399
                     /locus_tag="J8C37_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5305999.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3414..3845
                     /locus_tag="J8C37_002591"
     CDS             3414..3845
                     /locus_tag="J8C37_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306000.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4027..4332
                     /locus_tag="J8C37_002592"
     CDS             4027..4332
                     /locus_tag="J8C37_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306001.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1608 a          703 c          929 g         1165 t
ORIGIN      
        1 tctccgactt ccaggttttt gaccattttc tctcaacgtg gaaaaaccgc cacttatgag
       61 agaaaatggt caaaaaccta agcgggaagt ctccgaataa cataccaagc gccgacagcc
      121 aacagataaa gaatacacta ctaaaaatag tagtagcata aaatttaaaa gggaaaaaat
      181 caacccctac cccagtcttg gggtctcaaa actaactccc ccctaacccc ctctttcagc
      241 ctcttttgaa agagggggag aatcaacccc acggccaacg ccccagtgtt cttggggctg
      301 tccagaacag aaacttgcga gagagtgggt gtaaaacaag tgtagaggaa acttgaagac
      361 ttgttcgaac acaaacttac aagagagggg agtaaagcag ttgcagagga atcaggaaga
      421 tttgttcgaa cagaaacttg caagaaagca ttattaaatt gaaggtgatg aaatatgaag
      481 tatttattgc atggctattt tattgattat tctactcgca atagtagtac tttagcgatt
      541 ctatgtttgc ttcatgaatt gagagtggtt actagaaaac agctatacaa actattgaat
      601 tttgacagat caattagtaa acaaggagta acgaatgctt tagtgagatt atatgaaaaa
      661 cagttaatcg atcgaaaaaa atatggaaca gaaatgtatt attatttgac gaaagaggga
      721 catcaaagca taggtgggta ttatacgttg ccgaaagttc ctgaatataa tttgaatcac
      781 catttacaaa ttaacaatta tttgattaaa ggaatggcat tggcaaaaga ccacccacat
      841 ttaaaacgga ttatttcaga aagacgtacc gtctatgaaa cgaaggattt aaatcctaag
      901 acaaaaggaa gaaaattttt tgtgccagat tttatttttc gttttcgaga caaagaaaat
      961 catgagatca attggtcctt tgaaattgag ctaacgttga aaacacgtcg tcgctaccgt
     1021 gaagcgattt ttccgaaata tattcgtgag ttgcgaacac gtcctgaatc acatttaatt
     1081 tatgtgacac ctagtggcgc aattcaagaa gaacttcatg cgtttaagcg tatttttttc
     1141 aagcgcgaaa ctgaaaaaga tgcgagtcaa caagaattgt ttgagcggtt gcatattttt
     1201 tcagctttag agtttgaaga gggattaaaa gcattattag agaatgatcc agcagtcaat
     1261 tggcggacag tagaacgaga aaaaatttat ccgaatggcg ctgttcaaag tcgtttgaat
     1321 ttatttgaag aatcaactga aaatgaggaa aaaccaaaag tgaagtttac gcctgaagta
     1381 ttgtaaaaga gggtgttcgc ttcgctcttt ttttgtgcca gtttgctggc accgttcaga
     1441 cgcaagcgtc tgaaccggct cgtttttagg aaggactaac ttgtttgatt tatccgttgt
     1501 caaagaagtg gacgttcgag agtccctaat cgtatagcag tcattcggcg agtataacct
     1561 aaaacataac ctgccaattg ccaagggtaa aatgcacgct gcgcgccctt ggcaatcgtc
     1621 agaacatgtt ttcgagccgg ttatgccgaa cgaccgctat acgaaaaggg cctctaaaca
     1681 gaagtccact ttgatccttg acaactgaaa cccaaaatca aacaagcagg ccttcgtcta
     1741 aaaacgagcc gaatgaagac cttgggggtt gcacccccaa acccccgtgc ccccgacaca
     1801 ctaccgaaaa ggtgggggtg ggtggcgaaa aaatcaaata aaacattcta ttcaagaaag
     1861 gaagaaacaa ccatgacaac taaaaacgag caactgaaag aacaggcaag aaaagagttg
     1921 caagaaagag gtttgattat tgaggggagc tttgaaggag attttgaaac ctatattggt
     1981 tgttatgcaa gaccgataaa caaacctaca gccttagacc caatgaacga acaagaagta
     2041 aaagaacaag aaaaacatgc agtgaatggt tttccgcaag atttcactga atggtatgag
     2101 tgggaaatta acaacgggaa gcttgaaaac ttcttataaa agtcagatga cgtgcaaaga
     2161 gaatgattct ctttgtacgg taaaaatata aagaaagaag gaaaaagaaa atggcaaaaa
     2221 acacacctta tcaagtagga gacatttttt atagtagttg gggctacgaa caaacgaacg
     2281 tgactttttg gcaaattgtg aagttaacag aaaaaacagc atggtttcag cctattcaaa
     2341 agaaaacggt cgagcattat tcgtccatga gtggaaaagt tgttccaatg aaagacaagt
     2401 ttatagagta tcctttagca aaaactaaag acagtattgt aaaaaaacga attgatccag
     2461 aaacctatcc tgatatgtta tgtggttatc gtagttggga cacgtttcat agatataaaa
     2521 atcaagctgt ttatgaaagt agttattatt aaggggatag tttccccttt caaaaataat
     2581 aaatagaaga atggagagaa aaaatcatga tgaattcagt gaatttagta ggacggttaa
     2641 caaaagaagt ggatttaaaa tacacaccaa aaggcaatgc gacaggcaca tttattttag
     2701 ctgttaatcg aaattatacg aatgcaaacg gcgagcgtga agcagattat atccgctgtg
     2761 tcatttggcg gaaagctgcg gaaactttag caaaatatac tggaaaagga agtttgattg
     2821 gcattaatgg acgtcttcaa acgaggagct atcagaatca gcaaggacag accatgtatg
     2881 ttacggaagt tctagtgaca gatttctatt tgttagagag taaagaaatc aatgagcaac
     2941 ggtcaaaatc aatggcgaat aatcaaaata attcttcaat tccaggaaat gagattcaaa
     3001 tttcagatag ttatttgccc ttttaagtga aaaataattt tgttctatac ctgatttctt
     3061 caggtataga acaaaaaata aatagagatg aggcgatttt ttatgaaaat cattgacagt
     3121 tacgatcaac gaaccatgca atatttgggc tcgtttgatt atacagatga aaatattatt
     3181 aattatgttg caagtttacc tccttatcaa agcgttcgct tggtagattt tgcaagtgat
     3241 gaattggtat taacaacgat gggcaacttt ttagattatg tcggagacaa aaaatggttg
     3301 gaaaaaattt tgtctaaatt gattgcgaaa caaacaggag aagaacccat aaatgcgatt
     3361 caattgttca atcgatatga acaagaaaac aatgattaaa gaggagagaa aaaatgaaaa
     3421 gattaaaacg aagtattttt attgtgctag cattgattga ttttgttggg acaggttgtg
     3481 aagcaattga cgttcaaaaa gctcccttat cggaaataaa acaagcaata acaaaacaag
     3541 atgaagaaca accgctaaat acacaagtag aaatagtgaa tcaaaataaa ccgagttttt
     3601 ctgaagaaga tttttcgtta gaaaatggaa ccaggcaatt tttttcagat ttggatcgtt
     3661 taaatcgggt aggtgtagca agtgcacttt tgcataaaag caggatgcca acagaagaac
     3721 gagaccgatt atacattaag ccaactggct ggaaacaaaa gaaaatgaag aatggcgatt
     3781 atctttataa tcgttgtcat ttgattggct atcaacgtgc gacaagtttc cccataagta
     3841 tgtaatacga aacgggagga ttaatcttaa gattaatcac aactaaccta tttgacagtt
     3901 ggaatgaaag ctatcatgtc tgctgaatca actggtctga tactcctaca tgcaataaag
     3961 tctactagga agttattgta tgaagctagg taaaggcggc agaacaattt ctaagtgtgg
     4021 aatcatatga agttggatat gccgggacgc tataaattac tcagggtgag actgttttta
     4081 attaaactga cgaacttgcg aatgtacggg tctataaccg acttatattc gaaagatata
     4141 agattgcgaa tgcaaagaac attatcgaaa agtaagaatg ttggtcatga aattcgagat
     4201 ggaagactag cggtgtatct gtcaagagtt ggtaaaacat gtttgattga ttcagatgag
     4261 agtgggaatg cgacaaaacg ttttacagaa gaaatagagc aactagcaga actgaatata
     4321 cgatattttt aaatacttta gcggagaata aaaaaatcct ctacagaaaa ctacggaggg
     4381 caacatgtgc tattgtttca acaat
//
LOCUS       DADOOS010000176         4401 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.182, whole genome shotgun sequence.
ACCESSION   DADOOS010000176 DADOOS010000000
VERSION     DADOOS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.182"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..329)
                     /locus_tag="J8C37_002593"
     CDS             complement(<1..329)
                     /locus_tag="J8C37_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306002.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADL"
     gene            complement(537..1463)
                     /gene="glsA"
                     /locus_tag="J8C37_002594"
     CDS             complement(537..1463)
                     /gene="glsA"
                     /locus_tag="J8C37_002594"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAZ5306003.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1486..2913)
                     /locus_tag="J8C37_002595"
     CDS             complement(1486..2913)
                     /locus_tag="J8C37_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5306004.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2944..4158)
                     /locus_tag="J8C37_002596"
     CDS             complement(2944..4158)
                     /locus_tag="J8C37_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAZ5306005.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1384 a          928 c          779 g         1310 t
ORIGIN      
        1 agatcagcca gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa
       61 aaataagatt gaccgtacag aaactgtgtg agctcagaaa gagcaatacc atgtttgaat
      121 acgagtttat acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg
      181 gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt
      241 tgttcgatgt ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat
      301 atatttcgct cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga
      361 ttttatttaa tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt
      421 tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct
      481 cattgtaggt agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat
      541 tcaaagatgt cagcgtctag ttctttgact acatctttca acaattggat accagctgcg
      601 ctgttaccaa atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca
      661 gctgccatca acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac
      721 tctcctgatt catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt
      781 tcttcagtaa tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca
      841 gctaattttg ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg
      901 agtatctccg gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag
      961 cgattgatat cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta
     1021 ggatcatcac agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct
     1081 ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga
     1141 tgtttgcgat gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc
     1201 gtgttgattg tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg
     1261 ggaacttttg acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt
     1321 tgttttgtat ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat
     1381 gctggaatgt aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat
     1441 tgttctaatt cttttacttc catttgaata accagctttc attttttatt tttctgcatg
     1501 ctggaattta tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga
     1561 aacaatcaga atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac
     1621 aaatgagata caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt
     1681 tacaactttt ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag
     1741 cttcaaatag cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga
     1801 catcgctgca agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca
     1861 gattgttgca acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc
     1921 ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg
     1981 agaggttacc caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat
     2041 tttcgcaatc catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat
     2101 cacccctgta ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc
     2161 aaaagtatct aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt
     2221 tttagggttt tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa
     2281 aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt
     2341 catagacagt atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa
     2401 caagactaat gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt
     2461 gtatttggtt ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc
     2521 caatttcaga tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc
     2581 agttaagaaa tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat
     2641 tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt
     2701 ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag
     2761 caaaccgcct aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata
     2821 ttcatataat gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt
     2881 ttttgcagga attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt
     2941 gtttcaattg agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt
     3001 tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata
     3061 ggcatacacg gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct
     3121 tggattcgtt agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa
     3181 tccgcccatg ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg
     3241 aaaatcaaca gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt
     3301 tgcttctaca taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag
     3361 tacgtcgaca aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat
     3421 cgcaaatgca agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg
     3481 aagtgcacca ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc
     3541 agctgccata agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag
     3601 tgtgaggata catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc
     3661 gaaatctcta aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg
     3721 gaaataaatc agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct
     3781 ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag
     3841 aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg
     3901 tctcatcaaa aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc
     3961 caatccgaat ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg
     4021 agacatgatc gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc
     4081 aggagtcatc aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt
     4141 gataggtaat gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt
     4201 gtagttgttt ttttcttctg tttttttgaa ttaatacttg catttataaa agacagacaa
     4261 ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa
     4321 taaacggtct tctttttata gaaaaccgtt tattttcgtc tttttttatt tttaaagata
     4381 ttttttcata aggaataaag a
//
LOCUS       DADOOS010000177         4384 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.183, whole genome shotgun sequence.
ACCESSION   DADOOS010000177 DADOOS010000000
VERSION     DADOOS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.183"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(238..2010)
                     /locus_tag="J8C37_002597"
     CDS             complement(238..2010)
                     /locus_tag="J8C37_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5306006.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1964..3718)
                     /locus_tag="J8C37_002598"
     CDS             complement(1964..3718)
                     /locus_tag="J8C37_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAZ5306007.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3828..>4384)
                     /locus_tag="J8C37_002599"
     CDS             complement(3828..>4384)
                     /locus_tag="J8C37_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAZ5306008.1"
                     /translation="YIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGII
                     FVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRA
                     IPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIIS
                     VLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1489 a          820 c          609 g         1466 t
ORIGIN      
        1 caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca
       61 attttttaaa actttgatta ttccttatat tataagaata gacattaagt gaactaattc
      121 aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt
      181 tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca
      241 tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag
      301 atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt
      361 tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat
      421 aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt
      481 ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa
      541 agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc
      601 gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg
      661 agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa
      721 aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga
      781 agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc
      841 atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa
      901 cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt
      961 atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga
     1021 tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata
     1081 aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc
     1141 tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc
     1201 tactagaatg actactgcta aactaacata tgagaaaata gaggataagg ccatgactga
     1261 aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag
     1321 tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca attctatagt
     1381 ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt
     1441 ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc
     1501 taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc
     1561 cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga
     1621 catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt
     1681 tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata
     1741 taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac
     1801 ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat
     1861 tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt
     1921 agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat
     1981 tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat
     2041 gtttagccat taattcatta tgatttccac gttctactat tttcccttct gacaagacaa
     2101 gaatttcatc tacagattta atgctggata atcgatgagc tatcataata actgtttttt
     2161 ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg
     2221 cactggcttc atccataata acaatctttg catcttttaa aattgctctg gcaatagcta
     2281 tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc
     2341 tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt
     2401 tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata
     2461 gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat
     2521 ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta
     2581 gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt
     2641 gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat
     2701 tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt
     2761 gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt
     2821 gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca
     2881 acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta
     2941 taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg
     3001 ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca
     3061 aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat
     3121 attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg
     3181 tattctctgt cattttcttt aacaagaata caccaattac taacatggcg attagaacca
     3241 atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg
     3301 cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat
     3361 caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg
     3421 ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag
     3481 aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa
     3541 ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt
     3601 aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta
     3661 accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt
     3721 tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta
     3781 atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat
     3841 ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac
     3901 actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca
     3961 gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc
     4021 cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct
     4081 cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg
     4141 ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg
     4201 taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca
     4261 gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta
     4321 accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata
     4381 taat
//
LOCUS       DADOOS010000178         4282 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.184, whole genome shotgun sequence.
ACCESSION   DADOOS010000178 DADOOS010000000
VERSION     DADOOS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.184"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(37..1257)
                     /locus_tag="J8C37_002600"
     CDS             complement(37..1257)
                     /locus_tag="J8C37_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ5306009.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="J8C37_002601"
     CDS             complement(1277..1882)
                     /locus_tag="J8C37_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAZ5306010.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="J8C37_002602"
     CDS             complement(1931..2863)
                     /locus_tag="J8C37_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAZ5306011.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="J8C37_002603"
     CDS             complement(3284..4144)
                     /locus_tag="J8C37_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5306012.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DADOOS010000179         4266 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.185, whole genome shotgun sequence.
ACCESSION   DADOOS010000179 DADOOS010000000
VERSION     DADOOS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.185"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..281)
                     /locus_tag="J8C37_002604"
     CDS             complement(<1..281)
                     /locus_tag="J8C37_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAZ5306013.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="J8C37_002605"
     CDS             complement(730..1272)
                     /locus_tag="J8C37_002605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306014.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="J8C37_002606"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="J8C37_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="J8C37_002607"
     CDS             complement(2359..2721)
                     /locus_tag="J8C37_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306015.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="J8C37_002608"
     CDS             complement(2725..3033)
                     /locus_tag="J8C37_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306016.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="J8C37_002609"
     CDS             complement(3042..3461)
                     /locus_tag="J8C37_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306017.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="J8C37_002610"
     CDS             complement(3454..3687)
                     /locus_tag="J8C37_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306018.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4266)
                     /locus_tag="J8C37_002611"
     CDS             complement(3684..>4266)
                     /locus_tag="J8C37_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAZ5306019.1"
                     /translation="FIEPHFSRADIACDIIDVPDEFITQYRVVDPVSFKPIYGRSGKL
                     ETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQLRRGKATDWHEMVHE
                     SLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKKYRDLLKQESQNDELT
                     NHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1200 a          954 c          612 g         1500 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaac
//
LOCUS       DADOOS010000180         4235 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.186, whole genome shotgun sequence.
ACCESSION   DADOOS010000180 DADOOS010000000
VERSION     DADOOS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.186"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..893)
                     /locus_tag="J8C37_002612"
     CDS             complement(<1..893)
                     /locus_tag="J8C37_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5306020.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(886..1566)
                     /locus_tag="J8C37_002613"
     CDS             complement(886..1566)
                     /locus_tag="J8C37_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5306021.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1567..2349)
                     /locus_tag="J8C37_002614"
     CDS             complement(1567..2349)
                     /locus_tag="J8C37_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAZ5306022.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2342..3259)
                     /locus_tag="J8C37_002615"
     CDS             complement(2342..3259)
                     /locus_tag="J8C37_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5306023.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3423..3746)
                     /locus_tag="J8C37_002616"
     CDS             complement(3423..3746)
                     /locus_tag="J8C37_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5306024.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1243 a          932 c          745 g         1315 t
ORIGIN      
        1 accctccgca acttgacgga gatggagaag atgcccccgt ctaatctagc ggtcatctct
       61 cctcccattc cttccaccaa tttttttgac aggtaaagcc cgattccgct agattccgta
      121 tttgtgcggc tggtattttc ggtataaaaa cgctggaaga ctttttcaac agaggtttgc
      181 gttcctggtt tgataggatt ggccactgtt aatcgaatga atggatcctc ttttgaaaat
      241 gtcacctttg cttgtccatc tgcataacgc aataaattct gtatgatatt ttgcagcact
      301 cgcagcaaca tgagcggatc tgctaataca gttgtattta tcgagtgttc agcgaacgct
      361 acctcgatat ttttctcaga aaattgttca taaaaagaga aaaatacttc ttccacctct
      421 ttagaaaaag aaagcgtatg gagatccatt tctttttgct tcgtctctat tcgagcaaga
      481 tcataaaact gatccgtcat actgagtaat cttgcaacag attgtttgat gatcgttaaa
      541 tacttctccc gtttctccgt atcctctacc tgttccagta attgtacata accgttaata
      601 gcagtcaaag gagttctgag atcatgagaa aggccgacga tcgatagtct gatgttttct
      661 tccatttctt gaaatgcttg tcctttttgt tcattttcat ccaaccatgt attcagtgca
      721 tctactaggt ccattagatt cttttctcga aattcgactg aaagacgacc accatattct
      781 tgtcgttcag aaagagtggc gattttctta gttaatctct tgatttccct ttgcaggaag
      841 aaaataaaac ttcccataaa gataacaacg agaagcaaca gccatttaat caaatctaaa
      901 ccccactccc cacaccgtat gaatatacac agcatgtgcc tttgctagct tccctcgaag
      961 attggaaata tgcatattca ctgttttctc actatcaaaa tatggttctt gccaaactgt
     1021 ttcataaaga tttgctcgcg aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag
     1081 atcgaattct cttcctgtca aatggacagc cacaccattc acatttacat ccctcgactc
     1141 tttgtccagt aagatttcct tgtaatagat gcgttgcgac agatctttta cttggacaga
     1201 agtctgatga cgaagttgga tgccgattcg taacaaaagt tcttcgatat caaatggttt
     1261 cgtcacatag tcatccgctc ctgctgccaa caaggaaact ttatctcttt gatcgctttt
     1321 tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg atttctgcta acaatgcttc
     1381 acctgaaagc cctggaagca tgagatctag aaggatcaaa tcgacaggat atgtgcttaa
     1441 caataatttt ccttccgtac ctgaataagc atcaagaagt ttgtatctct gttcaagaac
     1501 ctcctccagt aaggaatgaa cagcttcatc gtcttcgata atcaaaatag tttccagcag
     1561 gaccacctac ttaatgtcag atttttggaa aataaacatt cctagagctg atgataccac
     1621 gattgttact agactgacca gaatcatttt cggtgcttgc gaataagttg cttgttgcgc
     1681 aaagtccgta tacatgagcc acgtttccgt aaaccactcc gttagaaatt ccaagccttt
     1741 gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg atataaagaa aagaaagaag
     1801 caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc actggaacag ataatgtccc
     1861 taatgtcagc agaagcaata caggaaatac ttttagaaca taatttccat agtttttgat
     1921 tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca agcatggcta gtcccgtgat
     1981 caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga agtttcgaca cataataagt
     2041 cattcgattc cgtcctaaag ataacgtatt gcgaatcgta ccgttttgaa attcttcccc
     2101 aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc catgcagcca ttttttctac
     2161 cattaaaatc cctgttgcac cgttgatgac tgtatccgtt gcttggttta attcattgcc
     2221 gtattttgta tgcatatacc attcgttcca agaataaaaa agcggaagta cgaccatcag
     2281 tcctagtgag acccacattt tacggctctt ccatactttt attttttcag cagttaatag
     2341 attaaacatg tggattgcct ccagttaatt gtaagaaata atcttccagt ttttgcccaa
     2401 ctaggtgaat accatccaca ggaacttttg cttctaccaa tgcttgattg atggctgcta
     2461 cttggtcaat tccttcaaaa agctggataa cacggctaga ttgtacaaaa taatccctat
     2521 aaccaagaga gtcgagaacc gttactgctt gttccgtcgc cgaagtgtca atttttataa
     2581 attgacgaga ttcttgtgcc aattcttctt tcgataattc tttgatcaag tgtccttcat
     2641 gcaaaatacc atagtgagtg gcgatctgtg acaattcatc tagcaagtgg ctagaaagca
     2701 agacggtgat gccccgttct tttaccaaac gttggatgat ttctcgcatt tctgccatcc
     2761 ctgctggatc catcccattc gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca
     2821 gtgcatttgc aatagctaaa cgctggcgca tacctaaaga gaaattcttt gctttctttt
     2881 tccctgtatt ttccaatcgc atcattttca gcaggtcttc gatttctcta tccgacacac
     2941 cgccgttcgc tgcttggata cgtaggttat ctcttgcagt taattctgga taaagagcag
     3001 gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg caaatccgtc gttccaaata
     3061 attcgatcga accttcatca atcgagatca aacccataat agttctcata aaggtagact
     3121 tacccgctcc attttcccca atcaatccat agatcattcc acgtttgatt tcgattgata
     3181 ctttatctaa tgctttggca gcaccgtatt ttttggttat accagttgct ttcaagaccg
     3241 tttcatttcc aaaatccata cttatccttc tttcttattg atgagtgaag tatagttgac
     3301 gggtataaag aaagcagcca gaaatgtata aagaaagtgt aaattttcca attgaatgtc
     3361 aaaaaaaaca atcggacaat acgtgtcttt cctattatca ggcagtttgt tatacgcttg
     3421 tatcagtttc ctgattcttt cttttcgacc aaagtcgttt ctctgtattc ctcttcactg
     3481 aaaatttttg cttctttcag atcaagcttg agtaatttta ccagaccggt tataaggata
     3541 actatagcaa gactccctcc cagaaaaatt agactgtctc gcagacgtgg ttgcgttgtc
     3601 ttccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac catggtaatc
     3661 gttgttatcg ttgacataag tcgtccttgt atatgcgttg gaacttctcg ctgaagccag
     3721 accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga aaaataatac
     3781 atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa gacttttcac
     3841 aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
     3901 tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa gctgaccact
     3961 tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta tctttttccc
     4021 gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa catagctaaa
     4081 aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg attaaggtaa
     4141 ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc gaacgataat
     4201 cgcaattaac gttttgagaa ctgtgaagct ttacg
//
LOCUS       DADOOS010000181         4231 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.187, whole genome shotgun sequence.
ACCESSION   DADOOS010000181 DADOOS010000000
VERSION     DADOOS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.187"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            11..733
                     /locus_tag="J8C37_002617"
     CDS             11..733
                     /locus_tag="J8C37_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAZ5306025.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="J8C37_002618"
     CDS             791..2830
                     /locus_tag="J8C37_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAZ5306026.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="J8C37_002619"
     CDS             complement(2939..4090)
                     /locus_tag="J8C37_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAZ5306027.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1538 a          783 c          818 g         1092 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa t
//
LOCUS       DADOOS010000182         4180 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.188, whole genome shotgun sequence.
ACCESSION   DADOOS010000182 DADOOS010000000
VERSION     DADOOS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.188"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            64..1365
                     /locus_tag="J8C37_002620"
     CDS             64..1365
                     /locus_tag="J8C37_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAZ5306028.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1392..1652
                     /locus_tag="J8C37_002621"
     CDS             1392..1652
                     /locus_tag="J8C37_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAZ5306029.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2651..3886)
                     /locus_tag="J8C37_002622"
     CDS             complement(2651..3886)
                     /locus_tag="J8C37_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAZ5306030.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1203 a          670 c          871 g         1436 t
ORIGIN      
        1 tgaacattct ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa aggagtggtt
       61 ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt
      121 ggtacacagc gtcatttatt agcaattcgt aatggggttg tttcaacatt gtcattgatt
      181 ttgattggga cattttttat ggtctttatc aatttaccgt ttcctggatg gaatgaattt
      241 attgcccctt attcggcaac gattgtttta ccctttagga tgacgatggg attgatggct
      301 atttatgcaa cttttgttat gggttcggat ctagctaaat catatggatt agatagtgtg
      361 acaggtggta tattatcttt aggaacgttc tttatgttac aagttcctgt aaacgtttta
      421 actcctgaag aggcacctct tggctgggtc ttacctatga gttctttggg agcatctggg
      481 atgtttgcgg ggatcttatc tatgattttt gcagtggaag tatatcgttt ctttaaaaat
      541 cgaaatatta ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg tagttttgag
      601 gcattgattc caggtgcagt cattttaaca attacttggc tgattcgaag cgtcgcgggc
      661 tttgatgtaa acgctgcctt attatcgtta ttcgagcctt taaccaatat tttagggaat
      721 aatttgcttg gtgttttact accaatgttt ttgatccatt tgttgtggtc atttggtatc
      781 catggcatga gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat gttggatgag
      841 aatgcaaagg cattggcaga tggtacagct gccacccaat taccctatat cgctcctgaa
      901 cagttttatc aatggacggt aacaattggt ggtgcaggag caacgatcgt ggtatctgtt
      961 ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt ttctattatt
     1021 cctggtatct tcaatatcaa tgagcccata atttttggag ctcctatgat gttgaatcct
     1081 tacatgttca tcccgttcaa cttagttcct ttagttttga ctattatcag ttattttgca
     1141 gtcaaattag aaatggtcaa tagatttact gttttgccaa catggacatt accagctcca
     1201 atcggcggtt atctttcagc aggaaatgac tggcgtgttg ttatcttgat catgatcaac
     1261 acattgatag catttattat ctattatcca tttgtaaaag cttatgataa aaagatgctg
     1321 tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg gtttttagaa
     1381 ggtgggaaac aatgtattgg ctgattttat tttttgtttt tatcttttta ttaacggcta
     1441 gtcaccttat attgaatatg ctagctacat atcatattca aatcaatcga tggatttggg
     1501 cactggcatc atttttgatc gtgattcttc caaagatcat tgtaccgcat atgaatgttt
     1561 tgttttcatg ggggacatat gtactttgtg gaatttttgc tatcaatttt atgatcgagc
     1621 agcatagatg gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg taacagaagt
     1681 ggttagcttc aagaaataag aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat
     1741 gttggcttat ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt
     1801 ttattcggaa tatgggactg tgacaaggct tttgtcacag ccctttttta cataaagatt
     1861 attattgttt taactgtttt ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa
     1921 tggatttaga attagtagat ctactaccaa agtgatcaag tcaaatatag atttgggagc
     1981 ttacaaccat atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag
     2041 tgtttattca aatcatgaaa cgtgtataga aataattgtg ctgggaatag caatcagtca
     2101 tggtgaacgt gacttttagt gtatcgttgc tgacgacata gaacattctg acatgatcta
     2161 ctcaatatgt ggggattgct ggcagtttat ataggagtat gcatcggatt gcgagatcat
     2221 tgtctcaaca gaagagggag gttgattatc aaagataaag tggtaaatcc tacatttgat
     2281 ttagtaattg ttgaggggac tagcggcaca aatctctaca aagcgatgct tctagatcta
     2341 tatgacacat tttaagaaaa tgtatttgcc taattattat ggtctttctt ttgaagaaac
     2401 gttgagatga tccaaccaga gaaaggtgtc aaagaatttg actaagaata aatggcagac
     2461 gctacctatg ctcatttgga tagcgtcaga tcttttattt taaggatgat tttgtgtttt
     2521 ttagacaccg taatttaagt aactctaact tttcaatcca ctatttactt tttttgtatt
     2581 ttcactgtct gggtctcgaa tagcgaaatt actgatgagt ctctattaaa aaagcttttt
     2641 ctacttgaaa ctatattggg tggttgctca ggaaggtttc gatttcttga attgcttgtt
     2701 gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg
     2761 aagaaacttc gcgcagagca atctgcagtt ttctttcagt cactggtttt gtgagggcga
     2821 ttgcgtttcc gtattttaca caatagtcaa tattacagct ttgctcaaat tgcatcccca
     2881 caccgataaa cggtttgccg gattcacagg cagtttggac ggtcccttcc ccgccgtgga
     2941 taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga cagatattct gttattaaaa
     3001 gatcttctga gtcagattga atgtcatcaa gcagatagtc aataggtgca ataaccgcta
     3061 gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac
     3121 tacccattgc gaaatagata atacgttttc tttcttgttg ctttcgctgt ataaaagtta
     3181 gcacttcttc aggcaaggaa gtactgagct tagcaaaaat tggaccaacg attcgataat
     3241 tttccggcag ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga tttagatcag
     3301 catccatcat ttgtaaactt gtttttggta aaggaagcat ttctttttgt gcagcgtgtt
     3361 tgaaggcatt ggggatccat cgggtattta ctatacgctt ttttgtccag tgccaaaggg
     3421 gattgcttaa agggccgaag aaacgtaact cgtggggcat cagcgcatgc tctgattgaa
     3481 aaaaaggttc actcaaagca tagggtttaa catatactaa aggaatcttt ttattgcggg
     3541 ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag tcgaggctca aacttagcaa
     3601 tcagactctg ttcactagtg atacgagagg caaccatttc tttggtaaaa gggtttttaa
     3661 gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg ttttttcgta aggatcggcg
     3721 ctagtaaatg atactcaaag ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc
     3781 caaagaatac acagtaataa cggtctttca tagctttggc aacctcaatc attcgtgttg
     3841 tctcagctaa attgaatact gcaggagcaa aaagaattgt cttcatgaaa tcctccttat
     3901 tggaatgagt ctatcccttc gtttcttact agtatactaa aaaacacagc gttcgagtag
     3961 cctatgtata ttttgaatat taaactgtct ctcgctgttt tcacaacttc taaatttaaa
     4021 ttttgtaatc aagttgaaac tccaattttc aaaaaaacac gcaacaattt tttgatcgat
     4081 ttatatgttg tgccagataa atacgagaag ttgtgtcatt aaaaataaac atgagaacat
     4141 aaaaaaataa aataattata ttaattgata ggtgtgctgt
//
LOCUS       DADOOS010000183         4076 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.189, whole genome shotgun sequence.
ACCESSION   DADOOS010000183 DADOOS010000000
VERSION     DADOOS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.189"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(1..354)
                     /locus_tag="J8C37_002623"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="J8C37_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="J8C37_002624"
     CDS             complement(614..1162)
                     /locus_tag="J8C37_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306031.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="J8C37_002625"
     CDS             complement(1163..2020)
                     /locus_tag="J8C37_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAZ5306032.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="J8C37_002626"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="J8C37_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="J8C37_002627"
     CDS             complement(2582..2911)
                     /locus_tag="J8C37_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAZ5306033.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="J8C37_002628"
     CDS             complement(2988..3260)
                     /locus_tag="J8C37_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAZ5306034.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="J8C37_002629"
     CDS             complement(3260..3523)
                     /locus_tag="J8C37_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ5306035.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="J8C37_002630"
     CDS             complement(3629..4045)
                     /locus_tag="J8C37_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306036.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
BASE COUNT         1192 a          827 c          507 g         1550 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta atacct
//
LOCUS       DADOOS010000184         4073 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.190, whole genome shotgun sequence.
ACCESSION   DADOOS010000184 DADOOS010000000
VERSION     DADOOS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.190"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            591..1280
                     /locus_tag="J8C37_002631"
     CDS             591..1280
                     /locus_tag="J8C37_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5306037.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="J8C37_002632"
     CDS             1267..2475
                     /locus_tag="J8C37_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5306038.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="J8C37_002633"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="J8C37_002633"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAZ5306039.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          703 c          812 g         1238 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DADOOS010000185         4034 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.191, whole genome shotgun sequence.
ACCESSION   DADOOS010000185 DADOOS010000000
VERSION     DADOOS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.191"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(67..609)
                     /locus_tag="J8C37_002634"
     CDS             complement(67..609)
                     /locus_tag="J8C37_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAZ5306040.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            744..1133
                     /locus_tag="J8C37_002635"
     CDS             744..1133
                     /locus_tag="J8C37_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306041.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1137..1901
                     /locus_tag="J8C37_002636"
     CDS             1137..1901
                     /locus_tag="J8C37_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ5306042.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2030..2977
                     /locus_tag="J8C37_002637"
     CDS             2030..2977
                     /locus_tag="J8C37_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAZ5306043.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3107..3847
                     /locus_tag="J8C37_002638"
     CDS             3107..3847
                     /locus_tag="J8C37_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306044.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1342 a          687 c          814 g         1191 t
ORIGIN      
        1 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
       61 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
      121 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      181 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      241 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      301 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      361 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      421 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      481 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      541 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      601 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      661 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      721 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      781 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      841 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      901 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      961 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
     1021 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1081 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1141 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1201 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1261 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1321 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1381 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1441 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1501 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1561 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1621 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1681 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1741 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1801 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
     1861 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1921 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
     1981 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
     2041 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
     2101 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
     2161 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
     2221 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
     2281 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
     2341 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
     2401 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
     2461 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
     2521 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
     2581 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
     2641 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
     2701 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
     2761 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
     2821 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
     2881 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
     2941 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
     3001 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
     3061 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
     3121 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
     3181 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
     3241 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
     3301 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
     3361 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
     3421 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
     3481 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
     3541 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
     3601 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
     3661 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
     3721 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
     3781 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
     3841 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
     3901 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
     3961 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
     4021 cgcagatcgt ttgc
//
LOCUS       DADOOS010000186         4025 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.192, whole genome shotgun sequence.
ACCESSION   DADOOS010000186 DADOOS010000000
VERSION     DADOOS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.192"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            852..1064
                     /locus_tag="J8C37_002639"
     CDS             852..1064
                     /locus_tag="J8C37_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306045.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1342..3141)
                     /locus_tag="J8C37_002640"
     CDS             complement(1342..3141)
                     /locus_tag="J8C37_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAZ5306046.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3265..3861)
                     /locus_tag="J8C37_002641"
     CDS             complement(3265..3861)
                     /locus_tag="J8C37_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAZ5306047.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1448 a          711 c          688 g         1178 t
ORIGIN      
        1 tattaaatat tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc
       61 ataaaaaacg agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga
      121 gagacaattt ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta
      181 caaacgtctt ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc
      241 ttatctagat tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt
      301 attttcgttt tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg
      361 gtattgcata tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag
      421 ttcttgaaag tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt
      481 gatgcaatta aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta
      541 ttttctttta atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata
      601 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
      661 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      721 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      781 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      841 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      901 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      961 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
     1021 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
     1081 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
     1141 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
     1201 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
     1261 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
     1321 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
     1381 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
     1441 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
     1501 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
     1561 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
     1621 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
     1681 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
     1741 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
     1801 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1861 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1921 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1981 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     2041 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     2101 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     2161 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     2221 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     2281 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     2341 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     2401 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     2461 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     2521 ccgaatggct gctcgaaaag gacgatctcg tagaatcatt tccggtaaag caaaaatcag
     2581 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     2641 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     2701 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     2761 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     2821 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2881 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2941 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     3001 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     3061 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     3121 gctacgtttc aaataattca tcctatcacc tgctcttcac attgtagcgc aaatctaatg
     3181 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     3241 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     3301 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     3361 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     3421 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     3481 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     3541 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     3601 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     3661 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     3721 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     3781 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3841 tcgagaggca gcaatattca ttattcatca actccttaga ggttctttgt taactatact
     3901 ttactagaaa agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt
     3961 aagttcatca taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta
     4021 ataac
//
LOCUS       DADOOS010000187         4024 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.193, whole genome shotgun sequence.
ACCESSION   DADOOS010000187 DADOOS010000000
VERSION     DADOOS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.193"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(133..1638)
                     /locus_tag="J8C37_002642"
     CDS             complement(133..1638)
                     /locus_tag="J8C37_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAZ5306048.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1635..2321)
                     /locus_tag="J8C37_002643"
     CDS             complement(1635..2321)
                     /locus_tag="J8C37_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5306049.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2514..3935)
                     /gene="gndA"
                     /locus_tag="J8C37_002644"
     CDS             complement(2514..3935)
                     /gene="gndA"
                     /locus_tag="J8C37_002644"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAZ5306050.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1106 a          852 c          690 g         1376 t
ORIGIN      
        1 ttaattaacg gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg
       61 aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt
      121 aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt
      181 cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt
      241 agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg
      301 ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg
      361 aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt
      421 agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg
      481 ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg
      541 atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc
      601 attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa
      661 gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg
      721 atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt
      781 ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat
      841 acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat
      901 ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag
      961 taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat
     1021 caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa
     1081 agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata
     1141 tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt
     1201 acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc
     1261 caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat
     1321 cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt
     1381 cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa
     1441 acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag
     1501 attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa
     1561 agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga
     1621 ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag
     1681 ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg
     1741 tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat
     1801 accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca
     1861 attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa
     1921 gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat
     1981 gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct
     2041 ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt
     2101 ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc
     2161 atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg
     2221 ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt
     2281 gccaagttct tttcatcttc aatgattaga atattgctca tttattattt tatcctttct
     2341 attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag
     2401 tcattttatc atggatagca attttaaaaa agagctgaag tctactcata acaatcgact
     2461 tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct
     2521 tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg
     2581 ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa
     2641 tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag
     2701 gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct
     2761 agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat
     2821 cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc
     2881 caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt
     2941 ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac
     3001 tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca
     3061 gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc
     3121 gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct
     3181 actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct
     3241 atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct
     3301 actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct
     3361 ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata
     3421 tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca
     3481 actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct
     3541 tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca
     3601 ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta
     3661 tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt
     3721 agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct
     3781 ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg
     3841 aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg
     3901 gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt
     3961 tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
     4021 attt
//
LOCUS       DADOOS010000188         3925 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.194, whole genome shotgun sequence.
ACCESSION   DADOOS010000188 DADOOS010000000
VERSION     DADOOS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.194"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(573..1130)
                     /locus_tag="J8C37_002645"
     CDS             complement(573..1130)
                     /locus_tag="J8C37_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306051.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="J8C37_002646"
     CDS             complement(1309..1920)
                     /locus_tag="J8C37_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306052.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="J8C37_002647"
     CDS             complement(2033..2290)
                     /locus_tag="J8C37_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306053.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="J8C37_002648"
     CDS             complement(2887..3165)
                     /locus_tag="J8C37_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306054.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="J8C37_002649"
     CDS             complement(3199..3450)
                     /locus_tag="J8C37_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306055.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DADOOS010000189         3920 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.195, whole genome shotgun sequence.
ACCESSION   DADOOS010000189 DADOOS010000000
VERSION     DADOOS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.195"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1383
                     /locus_tag="J8C37_002650"
     CDS             <1..1383
                     /locus_tag="J8C37_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306056.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
     gene            1543..1989
                     /locus_tag="J8C37_002651"
     CDS             1543..1989
                     /locus_tag="J8C37_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306057.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1991..2128
                     /locus_tag="J8C37_002652"
     CDS             1991..2128
                     /locus_tag="J8C37_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAZ5306058.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            2165..2458
                     /locus_tag="J8C37_002653"
     CDS             2165..2458
                     /locus_tag="J8C37_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306059.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2455..2679
                     /locus_tag="J8C37_002654"
     CDS             2455..2679
                     /locus_tag="J8C37_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAZ5306060.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2676..3695
                     /locus_tag="J8C37_002655"
     CDS             2676..3695
                     /locus_tag="J8C37_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ5306061.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         1367 a          693 c          812 g         1048 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taacttacta
      481 gatgaccctt actgggtagg taaaacatcg ttgggtgaga atattgcaaa taaaggtacg
      541 acgttcccaa ttaaatctag cgcgtacgaa atatataaag gtaacacgga agaagagctt
      601 ataataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt
      661 gtagcatata attattggaa cgttaatttt ggagaactaa aaccagttga gggattgaca
      721 gacgtatggt ctctaacatt cacaccaacg aaacttgaac cgggtttgcc taaagacctt
      781 cgcatttttc agttgcctaa agaaacggcc ggcgcatgcc aaattgactg gctcaaaatt
      841 gaaaaaggcg acacccgaac cccgaatatt agtcagttta aatactttgg tgaaggattg
      901 aaagatagca acaatccgaa cgactacagc tgggacatca cgcctgaata tactgaaaaa
      961 ggcttgaatg atacggttag tttgactgaa ccgcaatcca tcgaaggatt gaaaaatttc
     1021 gaagatggta ttcaatcaaa aggaaaatct gtattgacat cagacgacaa caaatatgaa
     1081 gtcgtaacct taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga
     1141 aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa gcagtggtgg aaatgattca
     1201 acgatactaa caattccaga aggctatcgg ccaccaatta gttttgagca actggttggc
     1261 tcgatagacc gttctacttt gaacagtgct cagttatcta ttggtgcaga tggagccatt
     1321 aaatggcgaa gaaactcaag ttatggatca gcttattcat ttgttatcac ttattcaatt
     1381 taagggagga aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca
     1441 aatcattgtg aatgacgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat
     1501 tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa
     1561 tatggccgta ctggcggaga gtatgcagga aaagtattgg acgacatgct tgtatccgtt
     1621 ccttacacag atcagccacc gctcgaaggg gttcgttcag atggcgaacc gctaacgatt
     1681 gctgatcaga tgtttgatcc taaattgaac caatggatta ttttagcgaa cgcgttagat
     1741 cacaacgatt taaacaatct caaagcgatg tacgaggctc ttgaacatga aaacggcaac
     1801 ctaaaacagc taaatgccaa actcatgcta agcgatgtag cgattaaaca ggaaaatact
     1861 gcattgaaag aaaaagctga cagtttagca caaatcaatt caaaaatgat gcttgcttcg
     1921 ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaagga
     1981 ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac
     2041 tgacgatcaa gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat
     2101 cattaataaa gataagagcg catcttaatt gatgcgtttt tatttaaggt aaaggagttg
     2161 tcacatgatt aatttagggg aatggggaat gatagcagga tcaataaccg ctatcgtttc
     2221 tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta ctgagactct
     2281 ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
     2341 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatacaga
     2401 aaaattggtg gaacacacgc aacaaatcaa aactttgttt agggagagaa gaaaatgaat
     2461 aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc actagctact
     2521 ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg tgttgtcatc
     2581 attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg aacctacaaa
     2641 atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt actattactg
     2701 cgatgagcct gttaacggct ctttttttat tgccaattaa cggatttgcc tatactatca
     2761 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtata
     2821 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
     2881 aacgttcatg gactagcgct tatactgctt atattgtggg agacggcgga attgtttacc
     2941 aagtcggaca acctggttat gtacagtacg gtgctggttc atatgctaat gccaatagtc
     3001 ctgtgcagat tgagttacaa cacacacatg ataaagcaac ttttgaaaaa aactataaag
     3061 catacgttga attggctaga gattcagcaa tgaaatatgg tattccatta acattagaca
     3121 cgccttataa ccaaccagga atcaaatcac atttatgggt aactcaaaac atttggggcg
     3181 atcacactga tccgtatgga tacttatcag aaatgggtgt aagtaaggaa aaactggctt
     3241 acgatttagc tcatggattt acagacgaaa acccaacgac ttctgagaac aagcctgtca
     3301 ttgatccaac ccgagcaggt gcagcaaatc caactttatc agatggaacg aaccattctc
     3361 acattgatca gtttggagaa atcgaaaatg cgaacttaca cgtcgctgga tggcacatcg
     3421 ctaactataa gtacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
     3481 gagtaagagc tgatggaatt tatagaccgg atgtaaatca agcttataat acttctggaa
     3541 atgttggcta tcatgtatct tttaacatgc gtaacttccc taataagaaa gtctatgtca
     3601 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
     3661 acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcatt
     3721 acataactat attgacaact ataaaaatca ttcgataaaa tagtgatgtt atcgcatatc
     3781 ttcactatca cccataatag tcacactcca agctatgcga taacaggttt gttgccacac
     3841 attctactgg ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt
     3901 ggtcagagca gacggctcat
//
LOCUS       DADOOS010000190         3848 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.196, whole genome shotgun sequence.
ACCESSION   DADOOS010000190 DADOOS010000000
VERSION     DADOOS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.196"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            131..505
                     /locus_tag="J8C37_002656"
     CDS             131..505
                     /locus_tag="J8C37_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ5306062.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="J8C37_002657"
     CDS             complement(619..1248)
                     /locus_tag="J8C37_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAZ5306063.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="J8C37_002658"
     tRNA            complement(1382..1454)
                     /locus_tag="J8C37_002658"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="J8C37_002659"
     tRNA            1668..1741
                     /locus_tag="J8C37_002659"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="J8C37_002660"
     CDS             1696..1845
                     /locus_tag="J8C37_002660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306064.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="J8C37_002661"
     CDS             2338..3666
                     /locus_tag="J8C37_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAZ5306065.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT         1313 a          596 c          714 g         1225 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggattaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaatt
//
LOCUS       DADOOS010000191         3770 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.197, whole genome shotgun sequence.
ACCESSION   DADOOS010000191 DADOOS010000000
VERSION     DADOOS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.197"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            251..484
                     /locus_tag="J8C37_002662"
     CDS             251..484
                     /locus_tag="J8C37_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAZ5306066.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            481..888
                     /locus_tag="J8C37_002663"
     CDS             481..888
                     /locus_tag="J8C37_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAZ5306067.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(2025..2543)
                     /locus_tag="J8C37_002664"
     CDS             complement(2025..2543)
                     /locus_tag="J8C37_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAZ5306068.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            3661..>3770
                     /locus_tag="J8C37_002665"
     CDS             3661..>3770
                     /locus_tag="J8C37_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5306069.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1306 a          626 c          576 g         1262 t
ORIGIN      
        1 atgctctaaa atttattaat ttgtttcaaa aaaagcatat aaacaggtat acgtttaata
       61 atttaaaata aatggtatac ataaataggg aaggaaatag aagggagcaa tctaaatttt
      121 ttgaattttc ttaattaatt atatagaaag aacaatcttt ctatagagtt agacatgaga
      181 gggactatgc ttgatttaaa atcaacaagc gtatatactg tatatacaaa gaaaaaggag
      241 ggagaagact atggcaatta aagaaagaaa atttagggaa gtggggaact cagtagtatt
      301 aactatttca aaggagtttc ttaaagaaaa tgggttaaat cccggagata cggttttttt
      361 agatgaagaa aaacttcgag cggcaattac aaaaaaacaa gccttatctg atgaaattga
      421 tttagcaata tctcaagcat taactgaata tgatactact tttagagagc ttgtcgataa
      481 atgagtattc attacctaga agccaaagat attataaaaa tgaatgtaat ccaaataaaa
      541 aaatactctc ctaatgaacc ccttggtgta aaggatgcga atgctttaga aatgtgtgta
      601 gggcaacttc gagcctcagc ttttggtgaa gaggtttatc cttcgattta tgaaaaagca
      661 gctatcctac ttatacaatt gataaagaaa catccttttc acaatgcaaa taaacgtact
      721 gctttcttag ctacttttgt ctttttgaaa atcaatggac agcttttagc tattgatcaa
      781 aaagatgctg taaatttagc ggtatatatt gctacgtatg ataaggattt cgatcaacta
      841 aaatatgaag tgatcaacac tataaagaca catacgcagc ccatttaagg agttactgta
      901 tgtgtcttta tagggttatt agctgctata gcgaaataat tgaagtaaat tacaaatagc
      961 tataattcaa atccaactat atagaaaacg taaattttct atatagttgg attaagataa
     1021 cgactattta ttttatcata tttttactat accctatact tttacttggt atatttatga
     1081 ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa aaatactaaa
     1141 gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca
     1201 cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa
     1261 gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc
     1321 ttgcagaaat cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt
     1381 tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt
     1441 caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag
     1501 gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt
     1561 agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca
     1621 atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc
     1681 atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa
     1741 aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga
     1801 tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa
     1861 attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac
     1921 ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat
     1981 acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc
     2041 ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata
     2101 aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt
     2161 gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta
     2221 cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac
     2281 ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat
     2341 ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct
     2401 aaattttcag caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga
     2461 atagtttcaa attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct
     2521 tctttatttt tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa
     2581 ttcatctata atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca
     2641 ttgactgtct aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa
     2701 atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg
     2761 actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac
     2821 taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt
     2881 cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat
     2941 tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg
     3001 acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac
     3061 tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta
     3121 ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg
     3181 cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg
     3241 taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata
     3301 attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat
     3361 tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg
     3421 ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg
     3481 gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct
     3541 gctcatttca acttatatgt tttagtgaac tacccccact tagcttggat aggctacaat
     3601 taactagaca gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg aggaattttt
     3661 atgtctaaga gaacacgaag aactttttca caagaattca agcaacaaat cgtcaatctt
     3721 tacttagctg gaaagccacg tgtagaaatc attcgagaat atgaactaac
//
LOCUS       DADOOS010000192         3715 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.198, whole genome shotgun sequence.
ACCESSION   DADOOS010000192 DADOOS010000000
VERSION     DADOOS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.198"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="J8C37_002666"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="J8C37_002666"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAZ5306070.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="J8C37_002667"
     CDS             complement(2261..2512)
                     /locus_tag="J8C37_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAZ5306071.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="J8C37_002668"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="J8C37_002668"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAZ5306072.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="J8C37_002669"
     CDS             3462..3680
                     /locus_tag="J8C37_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306073.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1027 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaa
//
LOCUS       DADOOS010000193         3714 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.199, whole genome shotgun sequence.
ACCESSION   DADOOS010000193 DADOOS010000000
VERSION     DADOOS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.199"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            127..579
                     /locus_tag="J8C37_002670"
     CDS             127..579
                     /locus_tag="J8C37_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306074.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="J8C37_002671"
     CDS             593..1282
                     /locus_tag="J8C37_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306075.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3714
                     /locus_tag="J8C37_002672"
     CDS             1407..>3714
                     /locus_tag="J8C37_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAZ5306076.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFS"
BASE COUNT         1251 a          489 c          772 g         1202 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctg
//
LOCUS       DADOOS010000194         3643 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.200, whole genome shotgun sequence.
ACCESSION   DADOOS010000194 DADOOS010000000
VERSION     DADOOS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.200"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            75..470
                     /locus_tag="J8C37_002673"
                     /pseudo
     CDS             75..470
                     /locus_tag="J8C37_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="J8C37_002674"
     CDS             790..1104
                     /locus_tag="J8C37_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5306077.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="J8C37_002675"
     CDS             1208..2386
                     /locus_tag="J8C37_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAZ5306078.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="J8C37_002676"
     CDS             2789..3082
                     /locus_tag="J8C37_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAZ5306079.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3283)
                     /locus_tag="J8C37_002677"
     CDS             complement(3170..3283)
                     /locus_tag="J8C37_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAZ5306080.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         1248 a          531 c          712 g         1152 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aat
//
LOCUS       DADOOS010000195         3557 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.201, whole genome shotgun sequence.
ACCESSION   DADOOS010000195 DADOOS010000000
VERSION     DADOOS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.201"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            1..492
                     /locus_tag="J8C37_002678"
                     /pseudo
     CDS             1..492
                     /locus_tag="J8C37_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="J8C37_002679"
                     /pseudo
     CDS             712..1008
                     /locus_tag="J8C37_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="J8C37_002680"
     CDS             1171..1617
                     /locus_tag="J8C37_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306081.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="J8C37_002681"
     CDS             1619..1756
                     /locus_tag="J8C37_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAZ5306082.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="J8C37_002682"
     CDS             1794..2087
                     /locus_tag="J8C37_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306083.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="J8C37_002683"
     CDS             2084..2308
                     /locus_tag="J8C37_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAZ5306084.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="J8C37_002684"
     CDS             2305..3330
                     /locus_tag="J8C37_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAZ5306085.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         1247 a          616 c          737 g          957 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcata
//
LOCUS       DADOOS010000196         3498 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.202, whole genome shotgun sequence.
ACCESSION   DADOOS010000196 DADOOS010000000
VERSION     DADOOS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.202"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(465..1028)
                     /locus_tag="J8C37_002685"
     CDS             complement(465..1028)
                     /locus_tag="J8C37_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAZ5306086.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1043..2086)
                     /locus_tag="J8C37_002686"
     CDS             complement(1043..2086)
                     /locus_tag="J8C37_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306087.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2219..2623)
                     /locus_tag="J8C37_002687"
     CDS             complement(2219..2623)
                     /locus_tag="J8C37_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAZ5306088.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2856..3491)
                     /locus_tag="J8C37_002688"
     CDS             complement(2856..3491)
                     /locus_tag="J8C37_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAZ5306089.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT         1082 a          718 c          587 g         1111 t
ORIGIN      
        1 aaagcactaa aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca
       61 tcgaattgct taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg
      121 tggattgcga tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct
      181 ttcttggata tgactattta ctactacttt actatctttc attcttcttg tctcacttat
      241 gataaggcgc tgcgtatcat gagtgaatga tattaggaaa acttcataaa ataaatcttt
      301 tatacatttc cacttctaga tggtcattca tgttagaaga atcaaactat ttttttactg
      361 tcgtaataat attaaaacac tagaacaaat caattaaatt ccataaaata taaataatag
      421 acctagcatc atctggtata aaatggatgc taggtccgtt atatttactc aaaattgatt
      481 ctacgaatag atcttcttat catttctatt ttagtagtca tgcgttgttg ttcttctaaa
      541 ggtggtaatg gtataagtag tttagataaa tttgtaccat ttacatttgg ttgcccagtt
      601 ccgtaagaaa ttttttctaa taacttccaa tataatggcg agtctaagaa gcagtctacg
      661 tattctgata ataatttctc aactaaccta attcgaatca agtaagaagc aaaaacagat
      721 tcctcactta tttccttaac caagaaactt tttcccatag ttcctcctgt ccttgctatc
      781 aaaatgtcat tttcttccaa tcttaaatca actagtttag aattactaat atcacagtaa
      841 ggcacactgg accaatttac acgtccctct tgtatatctg tgattctcaa aaacttaaca
      901 ttgccttgtt ttttcgccga atctgtatag ccatattgaa tcttagtaga aatacttcct
      961 aaataaaccc atagtcgaac atcgctagat gatttttctt gtcgttcttt atgaacaaca
     1021 gtgatcatct tgagttgtcc gactattttt tagttgcagc aatctttttg ggacgcttat
     1081 ttgcatgaag caacgttgct aagtctagta ataaaacatt ggctaacaca ggattggctt
     1141 tctcgcgata cgctgcggcg ccactttcaa acaatactgc tagacgatct tctacaacaa
     1201 cgatttgttc caaatacggt ggaaatttga ttcgttttag gatccggcta ggagagtccc
     1261 gctcggacac taagagttcc gaggtataag gaccataaga acgactggct aagagataat
     1321 ggccataaaa agccacacct tgaatcttat cagatgtttt tctgctcatt gtcacggttg
     1381 gttggatttt gtctttgcgg cgaattgctg ttttcatttc agctatcgtc ggcaatagat
     1441 agaaagtcaa ctgtccttca ccttttagtg tgaagttgcc agataccagt tgatcattgt
     1501 tttgagtcaa atacgatgct tgtggaatct ctgagagatc agttgtcagc caataagcga
     1561 ttggctgtcc cgtctcttca gatgtatctg ctaagatatc gatcaaacgt aaagcggcta
     1621 ctcgtccagt agcagatcct gtgattgtca accataaaag ttcccgtttt gtatcatata
     1681 ctaagccccc tacatgtggc cgtttaggta aaatcagcag tttgatcggc tgtcctgttt
     1741 ctctatccag tacatgaata atagaatgat gctgatggtc atagcagtag gcagaaataa
     1801 acacaaaatc ttgagaaaaa gccaaccctt gcggcgtcat ccattgacaa acctcatttt
     1861 cgccggtttc cggcaccacc attcttgttt ctgttaatcc aggaatgtta tattgttttg
     1921 cttcagcctt ttcccaagct gcttgaacaa taggataccg ttttaacttt ttttcaatat
     1981 cctgatgtat ccgttgctgc ttccaatgct taaactttag gacatagtaa acaagaaggc
     2041 caatgaccag caacactaaa atcattattg ctacccgctg tgtcattttt ttgcctcctt
     2101 gttctattcg atcaatcgtc catataccgc tattaattac cgtaactaca tgtttctttt
     2161 actagcgttt ctttatagga tctttcataa tctcatttat agtaacatta agaatccctt
     2221 attctactat agtcacttcc acatcaatat tgcctcttgt tgctttagag tatgggcaaa
     2281 atgcgtgggc ttcttctgcg attttctctg cttggtcctt ctcaagtcct ggcaaagaaa
     2341 cttctaaaac gaccccgatt tggtagcccc catcttctaa ttgaaataaa gatacccgtg
     2401 ctttgacttc agaatcagac tcaagattat tttcttccag catgtgttga accgcgccgt
     2461 tgaagcaaga tgcgtatcca gccgcaaaaa gttgttccgg atttgtccct tcactatgga
     2521 ttcctgggcg gtcgatcttc atgtgcatac ttccgtttgg tgcctcaact tcgccatcac
     2581 ggcctccacg attgatcatt gctgtttcgt acattttttt cattgagatc atcctttctt
     2641 tcgtcgttct atattaagtg taacgagtat tttttaatct gacaaaggta atgcttgcat
     2701 tcggcgacat cagttagaca acatcgatga tcagtgatag aagcagttta tctatacaaa
     2761 aaaagaaggg ttctatgata tttggtgtcc ttactcgcct tgagtaacaa caccaaatat
     2821 catagaacca cttaatcagc ttcacactgc tttttctact ttcgtagttg aaaaaaactt
     2881 aaacaaatca tcggcaaata gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc
     2941 ttaaagaagg caaactgact aatcgacgga atccgtgcta atccgtaaag acctgcaatt
     3001 agtaaactaa taactgttaa aacaagataa aaatggctaa gcgtaccttg accactttga
     3061 cgataaagca gaacaagtaa caatgggaaa agtagaaagc ctgcatagcc tagcccagaa
     3121 cctatattat ggatccaagt cgatagatta caagagcttt cattcgtatt gatactgaat
     3181 aagccagtaa aaatacaatc acctatccca tataaaccaa ctgttgcaac aacaagtatt
     3241 gcaagtgttt ttgatgttcc aacaaatagg tgatacaacg caggcaacga aagaacaaaa
     3301 aaaagtccag atacaactga ccagaccaag aaagcacgtc tgactggact atctacgtca
     3361 ccaaacacac taattacggt agtcatttga ttcagtttgg gataaaaaag gccaaggata
     3421 tacggtgtcg ataaatcact catcacacct aataataaaa aataaaaacc atatttttgc
     3481 aaaaaaatca ttttcttc
//
LOCUS       DADOOS010000197         3476 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.203, whole genome shotgun sequence.
ACCESSION   DADOOS010000197 DADOOS010000000
VERSION     DADOOS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.203"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(775..1731)
                     /locus_tag="J8C37_002689"
     CDS             complement(775..1731)
                     /locus_tag="J8C37_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306090.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1840..2085)
                     /locus_tag="J8C37_002690"
                     /pseudo
     CDS             complement(1840..2085)
                     /locus_tag="J8C37_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2230..2652)
                     /locus_tag="J8C37_002691"
     CDS             complement(2230..2652)
                     /locus_tag="J8C37_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306091.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2791..3221)
                     /locus_tag="J8C37_002692"
                     /pseudo
     CDS             complement(2791..3221)
                     /locus_tag="J8C37_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1067 a          738 c          560 g         1111 t
ORIGIN      
        1 ttttttatgt aaagttctgt caaatatagt cactttatat tactttctct tgagtaaaaa
       61 aacatgtgag gtcgtaaaca aagattgaac tatctacatt catactctat aatctgttta
      121 tttgcagact gataatagca aaagaaaact tataatcaaa taatatcaac atttgatcac
      181 ataatttgag attgaatgct gatttaccta tgttttacgt aatggtcttt agttattttt
      241 gctcataaaa tgcataaaac acgcgttttt atgtgtgctt gaaatttctt ggctctataa
      301 tttctaagac tgataaaaat attctatcgg ttctttttgc aaataaaaaa acatatagaa
      361 gctccattag attgaatcac cacaacacaa tcaaaggagc attccgtatg cttgattttc
      421 attctataaa actactttta ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag
      481 aatatccgaa gtaccattgt ttcatcgatc atgagaaaag cattcacctc tgtctgcaga
      541 tgttcttata tcgctctgtc agtcctatta ataaagtact gttttgtttt caaaaaaact
      601 attacgagta ctttttaaga gaatgccttg caaatattca tcacacaccg ttatataagt
      661 caaatagttt ctttcagtcg tcaagaaaag attctcctgt ccttggcatt atcagttaag
      721 tagcaactta attctcggac ttgttctact ctagcaaaat ttattttcat caacttacgt
      781 ccaaatgttt ttttgaataa ttacttcatc gcgggtctgg aagacttgcc tcattcgata
      841 agtcactagc ttttggcgtc cttcaggatt tctagtatac accaactgac atttgggcag
      901 cgacgattga tttaattcgt ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg
      961 ttctggtacc gtcagaacta tctttttccc ttcgattaaa taccgcggat tctccagcgg
     1021 tgctaaaagc tccctcatag cttcagtaaa aagctgtcct tctcgatagc cagcattctc
     1081 aagatagcat ctaaattcct gctgatcagc cgctacgatt acttttctat cactaacttc
     1141 accaaggcgc tgcaacgtcg tttgaacgca tatggccaag ctttttttgc gggcacattc
     1201 cttttcccat agcttttgtc gcctacgaat ttggaaatat tccagcaagt tacctttaaa
     1261 tatagctgac cagaagctta gaggctctat attttttact actatcggca atgaagtttc
     1321 ttgtttttgc tttacctgat cgactttcct aacgacgatt ggcatagata ggtgccagcc
     1381 atcagctaaa gcagtactcc attgttcttt caaccgactt cgttcacaag aaaaatagaa
     1441 catcgactga gtataagttt ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg
     1501 atcgccacca ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa
     1561 acgccgtagc attcttttca aatcaggctg ttcagacaga ggaactcccg gtaatccaat
     1621 cgctaaatgc caaatgttcg ttactttatt ttgtgcacct aggcgtaagc cccgccctcg
     1681 tagttgttgt gtttgcacaa aagtggaagc ttgattagcc aagatcaaca gattaattgc
     1741 aggacaatcc cagccttctc ctagcaaact aacggttcct accaaaatat tcaacttctt
     1801 ttcggttaat aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt catccaccgt
     1861 caaataaaaa taacccgaca tataaggatg ttcattcata cccgtcgcta gttctgccag
     1921 ctcaacagtt actagcattt tcggtaacag taagaactct ccacagatgg caccgatttg
     1981 ctgagtgtta cctaatagac gaacaacttc atcaaacaaa ggaaccacgc cataagctaa
     2041 ggtaccatca ctgcctgcta atacctcctt gcaaatacga tccatcaaaa tgaccgctgt
     2101 cagttcttgc ttcagctgaa cttcttcttt taacaaaatt tgacaaactg cttgcttttt
     2161 ctcaggaatc gcctgactac caacagtgtc tggatactct ggaaacaaat gcagcgactc
     2221 atcaaacaat catccagctt ttttcaattg ataaaaaata ggcaattccg gttgatcttc
     2281 aaacatctgc tgataaatag tgatcagctc ttttttacct aaagatggaa ctgaagcttt
     2341 tttaccttgc tgcttagaac tgcctaatag ctgccaatgt tcgggtatca attcatagtt
     2401 atgatgattc aagtaaatta atgcactagt gaacagctca aaattttcat aaatttcctt
     2461 cgaatgctca agcggatgct gaacaaattc taaagaaagc aaataatcca ccgtctcttg
     2521 gctttccatt aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa aataatgctg
     2581 agtagtttcc tgattagccg gaatcaaata tagaaaatcc tgatgaggac atagaacctc
     2641 ctcctttacc aactcgggaa tgctaatttc ttcgtcaatc aggccatttt aactcaatat
     2701 attgcttcca ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca
     2761 acagattcgg ttgttttttc ttcaagtcaa tcaacaattc accccagctt ttttttaaat
     2821 gatgagcttc atcaaaaaac taacagctcc agctgatcac cataagttgg ctcctccttc
     2881 agtcggttgt aaatggtctg atacgtttcc actgtaatat gactaggatg ttcaatcgcc
     2941 ccagaaagat agcaactatc tgctggattt actaagaaat attcctcgat tgttgttaac
     3001 cactgtttct tcaatattag ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg
     3061 atcatctcaa ttcctaaaaa ggtcttgcca gcccctggtg gagccactag atggactgaa
     3121 ttgttattta aataagtagc aaagttagtc aatatacgct tctgatagct acgccactga
     3181 attttttcat cagatagcca catcccaggt ccccctctca cgtattttct ctatcataat
     3241 ctaaatgaat acagcaaaaa aaagatttct tcaagaatat gtgtttttat agtaatattt
     3301 tacttactct aaactcaaat gaaaaggtaa aaaaactggt agtacaatct tttaaattct
     3361 cattgttttg tacgttcttt actatcaaaa aaacaaaaaa aacgcgtttc tacgcgtgac
     3421 caaaattttt agctctataa tttctaagac tgatagaaaa tcattctgtt tttgaa
//
LOCUS       DADOOS010000198         3419 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.204, whole genome shotgun sequence.
ACCESSION   DADOOS010000198 DADOOS010000000
VERSION     DADOOS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.204"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            77..1123
                     /locus_tag="J8C37_002693"
     CDS             77..1123
                     /locus_tag="J8C37_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAZ5306092.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1125..3278
                     /locus_tag="J8C37_002694"
     CDS             1125..3278
                     /locus_tag="J8C37_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAZ5306093.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         1064 a          669 c          663 g         1023 t
ORIGIN      
        1 aaaaaatatc tttttattta ctttttcatc cattatggta ttatttgaat atgatatatc
       61 attaagaggt gtttatatga ctcaaccatt gtaccagaca atatttaatg atttgcaaac
      121 ggctatttta actggtgaat tacctgtaga cagccaacta ccaactgaaa aagaactttc
      181 acgtacctat aaagtaagca gaatcacttc caaacgtgca ttgactgaat tagagcaatt
      241 aggacttatt tatcgcgttc gcgggaaagg cagtttcgtt aaatccgctt ctccggaaca
      301 gattcttgct tctcctacaa aaacacgacg gattctgttt cttcttccat atgtcgctga
      361 tttgtctgtt ggtgatttta gcaagggatt gaaccctgtg atgcaagata aacagattga
      421 tgtcatgatg acgacgcttg actttctaca aaataaaaca gcaaaagagc taatcaatga
      481 atttgacgga ttgatttatt atgctcttga tacagaacaa catcttgact tactttttga
      541 gctttcattg aaagaattcc ctgtcgtcat attagataaa aaaatctatg agttaccttt
      601 tcctaccgtt ctttccgata atttccaagg cggtgcgctt gctgcctctt atttgatcga
      661 acagggacat tcaaaaatcg cttatttgtt tggtacgcag actcatccac aatccgttcg
      721 acagcgttac cttggttacc ttgaagcatt ggacaaagct ggtttgactt tccacacttc
      781 actggatgac aaaagcgcga cattagatga attgctttct tatatcacta agtatgaagt
      841 aactgctttt gtttgtgaaa acgatgttgt cgctatccag accatgaatc tcttgagaca
      901 gcatggttat catatccctg aagatttttc aattatcgga tttgatgata tccaagcagc
      961 agctcttgtt gacccgccac tgacaaccat cgcacaagat tttgaacagt taggtaagct
     1021 tgcgggagat gctttggtca cttggttaga gacaaaagat cgaccatccg atagtaaatt
     1081 tccagtttca ttcatcaaaa gacaatcaac aaaggagaaa taaaatgaat atcggtcaaa
     1141 tagatactag acatgcaaca gcgaatcagc caaatttctc taatgggaac tgccagcctt
     1201 atactggcgt tccttttggg atgaattatt ttgcaccgca aactacggat caaaacggca
     1261 gctggtggtt tcatcctgat gaccgcgttt ttcaaggcta ccgtcttacc catcaaccaa
     1321 gtccttggat gggtgatttc agtcatatgc tgtttacgcc gatcaatgga aaactacagg
     1381 agaacaccct tttccatgct caaagttctt accgcccaga agaaagtata ttttgcccga
     1441 ctcatctgtc gatctgtcaa ttacgttatg gcatctgttc tacattgata cctagcatgt
     1501 atggcggaat cttatctatt gattacagca aaaatgacag cggtttactt ttatcctttc
     1561 cgggacgcca tcagctcttt gttatagatc cttacacagt ggaaggaaaa atcacacatt
     1621 ttgctggttc agaagatcct gatttttctt tttattttat attgagattt gaatatcctt
     1681 taacaattga tgagttagct gtttccaaca aaaatagcga ctcgatcccg ttgtattttg
     1741 aacagacaaa aaaacaaact atccgatttg ggacatcttt tatcagctcg gaacaagcag
     1801 cttataacct ggaactagaa caaaaccaaa gcaaagagac ttaccttgct cacagtcaag
     1861 aacaatggca gtcctatttt gatcgtatac agctcacaca ccatgatcaa caacaattgc
     1921 ggacgtttta tcataattta tatcgggtgt ttttatttcc acaaacattt tatgaaattg
     1981 atcctagtgg gcaaaaaatc cactatgata cgactagtcg atctataaaa cctggtgttc
     2041 tctatacaaa taatgggttt tgggatacgt ataaaacggt ttatcctctt tattctttga
     2101 ttgctgtaga aaaatacgaa gaaatgctgg aaggtttttt gaacagctat agagaaagtg
     2161 gctatttgcc aaaatggctg tctcctgatg aacgtggact tatgcctggg acattgatcg
     2221 atgcggtcat tgcagatgct gctgtaaaag atatccgacg agacttgatg cctgaatttc
     2281 taacggcaat ggaaaaaggt gctacggttc aaagcaaaca tccaaattat ggacgccaag
     2341 gaacagaaga ttacttgaca tatggctatg ttcctaatca ctaccacgaa tcagtcaatc
     2401 acacattaga ctatgcttat agcgattttt gtattagcca agttgccaag gtactagaag
     2461 aagaggaaat gacgaagaaa tatcagcagc aatcattgaa ctatcaaaat atctttgact
     2521 atgagtcagg atttatgcgt tcaaaagata aagatggcaa gttcagagaa ccattttcag
     2581 ctaatcgttg gggacaagat tatgcagaag gaagtgcttg gcaaagcagc tttgctgttt
     2641 atcacgattt ttcaggactg atcggaaaat atggtggcag tgagagatat aaagaaaaac
     2701 tcattgagct gtgcaatcag cccccaacgt ttgacccagg aggatatggt tttgagatcc
     2761 acgaaatgag tgagatggcc gcgatagaat ttggacaatt agctatttca aaccagccaa
     2821 gttttcacta tccttacttg ttcagttata taggacaacc tgaaatggca cagccactgc
     2881 ttaaacagtt gatgactcag accttcgacg attctccaat gggttaccct ggagatgagg
     2941 acaatggaag tatggccgct tggtatatct tcaatagtct gggattttat cctgtcacgc
     3001 ctggaaccgg tgaatatgtt atcggtatgc cattaattga caaggctgtt ttgcttttgt
     3061 caaatggaaa ggaattgacg attgaagcaa cgcctaatcg gccgcagcaa caatttatcc
     3121 ataaaatcaa acggatggac agagaatata cgaaattata ttttacccat caagatttgc
     3181 tgaaaggcgg cacgatctct taccaattag ggatcatccc tcaccctaag aaatatcaag
     3241 caaaagattt gcctttttcc ttatcaaact ataattaaca agaggttgtg aaaaagctgt
     3301 cctgcatcaa gtaataagaa cagccaagca aaaatagatc ctcatatttt tcgtttggtt
     3361 ggtcttatta ccgcagatgc aagcttttga acaccgttta ttgagaggtt gtgaaatca
//
LOCUS       DADOOS010000199         3334 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.205, whole genome shotgun sequence.
ACCESSION   DADOOS010000199 DADOOS010000000
VERSION     DADOOS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.205"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            15..1100
                     /locus_tag="J8C37_002695"
     CDS             15..1100
                     /locus_tag="J8C37_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ5306094.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1097..2989
                     /locus_tag="J8C37_002696"
     CDS             1097..2989
                     /locus_tag="J8C37_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ5306095.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2996..>3334
                     /locus_tag="J8C37_002697"
     CDS             2996..>3334
                     /locus_tag="J8C37_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5306096.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQ"
BASE COUNT         1272 a          436 c          666 g          960 t
ORIGIN      
        1 agaggtggga acatgtgaag gttcaaagga tagaagtgga gaataagccg tatccattgt
       61 atttattact agataaagaa taccagctaa tagaaccagt aatgaaattt attaaatact
      121 tagataatac tggtaagtct cctaatacca ttaaagcata ctgctatcat ttaaagttgc
      181 tgtacgagtt catggaacag agaggtgtta ttcttaatga tattaacttt gagttgttag
      241 cagacttcgt aggttggttg agatatcctt cagcatcaaa tgtaattgat cttcagtcaa
      301 aaaaagccat aagagaagaa acgacagtga atacaatttt aaatgtagtt atgagttttc
      361 ttgattattt aagtagatta ggagaattta aatcaattga tgtatttaaa caagcaaagg
      421 gaagaaattt caaaggattt ttacatcatg ttaataaggg tagataccaa aagaatgtct
      481 taaagttaag ggttaaaaag aaacagataa gaacattgag atcagaggaa gttaagcaaa
      541 ttattgatgc ttgtcatacg aaaagagata aattaatttt aatgcttatg tatgagggtg
      601 gtttaagaat cggtgaagtg ttatcgctta ggcttgaaga tattgtcact tgggacaatc
      661 aaatccattt aacacctaga gatgttaatg ttaatgaagc ttatattaaa ttaaggaagg
      721 aaagaacaat acatgtgagt aaagaactta tgtcacttta tacagattac ttgatatatg
      781 agtatagtga ggaattggag catgattatg tttttatttc cttaaaagaa ggctattttg
      841 ggaaaccact aaagtaccaa agtgttcttg atctagttag aagaatagtt aaaaggactg
      901 gaatagaatt tacatcacat atgcttcgcc acactcacgc aacgcagcta attagggaag
      961 gatgggatgt tgcgttcgtt caaaagagat taggtcacgc acatgttcag acaacgttaa
     1021 atacctatgt tcatctttca gatcaggata tgaaaaatga gtttaataaa tacctcgaga
     1081 gaaaggagca taagaaatga atgcttctag taaaaggaaa attattagtc agagtgagat
     1141 tagcaaaaaa atagctgtaa tgaatgaaga aatgcagggg ttttgggcta ataatagttg
     1201 ggatataaga aaatgtccac atccttctgc catagaatta agtaagaatc ctgctttaag
     1261 gaatcgttgg gttcgttttg aacgtgttaa aaatctgtgg ttaagaacag aattgaaata
     1321 tttttatttt taccatttaa acaatggaat atggaatgca aaaactgtct ggattagaaa
     1381 aggaacagta attaataaaa tgttagattt cttagattta aagtatccta gcattacttc
     1441 aattactgaa gttcctattg aaaaagcaat gacggagtat agaacttatt tgacaaaacg
     1501 gggtgttaga attaccacta ctaattataa gattactgct aatcaagaaa aaacacctgt
     1561 aaaagctaat tcctactatg ttactaatct aaaacaattt atggagtttt atgagaactt
     1621 ttattttgat ggagaggagt gggataaaga cgtttgggat agacgtaact tacctttgcc
     1681 agatgataag gttaacccaa cacaatatga atatacaatt aactttaaag ggtttcggaa
     1741 tacatatttt aaacaacttg taaaaagata ttgtaagttg agattgaaca tggatagctt
     1801 ttcctatgta agtgatattg cccaaagact taaagagttc tttaattttc tggacatgaa
     1861 atttaaacaa gttcagagag tacaccaatt aacgagagtg gaaattgaag catatttaag
     1921 tgaactaaac atgatgggaa taaaacctag tacaataact gggaggatct ctatattgga
     1981 aggactattt agtacccttc ttaggctaga atgggatgat gttccttcca aaatattaat
     2041 ttattctgag gactatccga aaataccaag agcaaaacca cgctttatag atgaattcgt
     2101 cctagagcaa ttgaacagtc atcttgataa attacccgaa tatatagcta cgatgactat
     2161 gattgttcaa gaatgtggaa tgaggataag tgaattgtgc accttgaaaa aaggctgtct
     2221 attagaggac aaagatggag atttcttttt aaagtattat caatggaaaa tgaaaaagga
     2281 gcatatagtt ccaatatcta aagaggtagc tttacttatt aaagttcggg aagataaagt
     2341 ttcagaggaa tttccagata gtgaatacct ctttccaaga aaagatggat cgccattaaa
     2401 acaagaaaca tttagaggtg agttaaataa attagcttat gagcaaaata tagtggataa
     2461 atcaggtgag atttatagat tccatgccca tgcctttcgc catacagtag gaacaagaat
     2521 gattaacaac gggatgcccc agcatattgt gcagaaattt ttggggcatg aaagcccaga
     2581 aatgacaagc agatacgctc atatctttga tgaaactcta aaaaatgaat ttactaaatt
     2641 tcaggaaaaa ctggttacca ataatggaga tgtgcttgat ctagatgaag ataatgaagt
     2701 cgatgatgta gagcttcaat ggttcaagaa aaatataaat gcacaagtgc ttccaaatgg
     2761 ttattgtaga ttgccagtag tagcaggtgg ttgtccacat gcgaatgcat gcttagattg
     2821 cactcacttc tgtaccagta agcaattctt accacagcac gaagaacagt tagagcgtac
     2881 agaagagtta ttagccatag ctaaggataa acaatggcaa agacaagtag agactaatag
     2941 ccgtgttaaa gagcgtttag aacaaatcat tggaagtttg acggggtaat tatcaatgga
     3001 taaacaagtt agaaatacaa cagaaattgt acgtttggcg aagcagaaat caaaaaagac
     3061 aagggaaaaa gtagacaaag cgatttctaa attttcgatt gaaggtaaag ttattaattt
     3121 taattcaata gcaaaggaag ctaatgtttc taaatcatgg ctttataagg aacacgatat
     3181 taggcaaaga atcgaatccc ttcgtgagcg tcaaataaca gcaaatgtag tctcaaaacc
     3241 caagaaaagt tctcgttcgg aggaaatcct tattaaaacc ttaaaaagaa gagtaatgga
     3301 attagaaaaa gaaaataaaa aattacagaa ccaa
//
LOCUS       DADOOS010000200         3313 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.206, whole genome shotgun sequence.
ACCESSION   DADOOS010000200 DADOOS010000000
VERSION     DADOOS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.206"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            107..415
                     /locus_tag="J8C37_002698"
     CDS             107..415
                     /locus_tag="J8C37_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAZ5306097.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            442..741
                     /locus_tag="J8C37_002699"
     CDS             442..741
                     /locus_tag="J8C37_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAZ5306098.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            915..1466
                     /locus_tag="J8C37_002700"
     CDS             915..1466
                     /locus_tag="J8C37_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAZ5306099.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1612..1905
                     /locus_tag="J8C37_002701"
     CDS             1612..1905
                     /locus_tag="J8C37_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306100.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1898..2194
                     /locus_tag="J8C37_002702"
     CDS             1898..2194
                     /locus_tag="J8C37_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306101.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2265..3269
                     /locus_tag="J8C37_002703"
     CDS             2265..3269
                     /locus_tag="J8C37_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAZ5306102.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         1254 a          458 c          656 g          945 t
ORIGIN      
        1 agtataaaat gaatcaaatt gaatatcaaa tatatattat atccatgaag cccaatcttc
       61 tttaatagaa gattgggcta tttttatggc aaaggaggga aaagaaatgt ttcaaaataa
      121 aggacaacgt tatgtcacaa agggagtaat ggacagtttg ccggtggaat tacaagcttt
      181 atgttggaat ctaatcgatc aaaacgttca aaagcaattt catttagact atcttcaaat
      241 ctttgaattt tctacggaaa aaggaaatca aaagttggtt catcgtcaag aagaaccaga
      301 agcaagaaaa gagtatctga tttcacctaa gcttcgtttg aaatcagtgt ctcaaaaaat
      361 atgggtcatt gattcgggtg agtatcaaac gatgttatta ccagaagaat actgatatct
      421 aaaagaaagg aacgaaagga cttgttagaa acggctatcg aagtactgga aaagtgtgca
      481 cagctagtca ctactcctga agagtggggc tatgaatccg ttacgatgga aaaagaggaa
      541 attgaaatgg gtatgcttcc tgaagatgtt actctaccac gtttagtgat gacacatctt
      601 tatatttatt gcgcaccaga agatggaaaa gactatgtag tttatttcat tacagatatt
      661 accagccagc gagaatttgt tcgtgggtta ctggtagaag gacgattggt ttggagtcag
      721 attggaggaa cgaatgaata agatcgatta tttgagaatg attgcgaaat gtttagaaga
      781 tatccttaca gcgattcatc aaattctgaa gaaaaaagga gaaagaatat gaaatatttt
      841 caactagtat tgacacttat aacggcaatg gtgattgaat tgcttgaaca aaaattagca
      901 taggagaaaa agagatgatt gaaaaagaat tagtgaccac acaaacagaa gtttggcgag
      961 atcctcatca tacccatcgc tatgttttca aacgacaatg ggcgatcaaa ggaaaagaag
     1021 cggaagataa attagcggta gtcattacta ttcgtccaac agatacagaa ccatttacga
     1081 atgatttgag tatgttatta atcgaaaaga atatccgaca attaggattt actggtttta
     1141 ttgctgtgaa tcttttttcg tatacaaaag cgaaaagtcc tcgggtattt cctcgtggaa
     1201 atgatgaaca atctttggaa atattaacaa ctgttttcac agaaaagaaa atcaacacca
     1261 ttatttttgc ttgtggttcg attacagcga cgagtcaagt ggcttatgaa caagcgaaga
     1321 ccatttacga tcaattgagt gcgaaacaaa aaaagatgac caaagtattg ataaatgcag
     1381 aaggtaagtt atcccaccca ctaagtattc atgttcgtaa tgaatggcaa ttggcagata
     1441 gtcaattatt atttcaagaa aaataacatt ttattttctt atgttactta taagtagttt
     1501 tacatgcttt ttgactttaa aaatacatgt gatgatacat gttatatgaa ttatcaatgt
     1561 acccagtaat atgctatttt cattatgtaa aaaataacag ggggtgtgta gatggagatt
     1621 gatgaaactt gtttgagtaa ctcttataaa agtatgaatg acttttttga ggaaatggaa
     1681 ctggattcag aattgctcta tcaattttat cttgcttttc gagggcaaca gatttctttt
     1741 ccaatgaaaa tgtacgatcg tgagttagtt agaaaaagaa tcgaaaacat gatagaacag
     1801 gaaaaaactg tagatatccg tcaattgaca gaagtttatg gtttctctgc aagatggatt
     1861 caaaaagtaa ttaaacaaaa agaaaggaga cgagtacatg gctaagctga ttgtattagg
     1921 tggtttaatt tacggtggat attggttata taaatatatg cttgcgcatc cgaatataga
     1981 taaaggaaaa gtaattaaga cagttggggt aatcatagtt ggactcatag ctctaactaa
     2041 gaaaaataat actaatagcc caaaaataat gagaacttat cgctgtagag gatgtggaag
     2101 ggaaatttct actagtttta atccatctga aagccctcaa ggaagagatc gttgtcatgc
     2161 tggctatcga cattcttggg aaaggattaa ctaaatgaac accattgaga ttaattgact
     2221 gataaaataa gtacgcgagg gagaaattga aagggggaaa taaaatgaaa aaatggttgt
     2281 tattactaat tggaatgggg attttattca ctggttgtac atctgagaat acgaggacta
     2341 aagagactgt agaaactgat acaacaattg aggctaaaaa aagtaaaacg caatcaactg
     2401 aacaaactac aacagaaatg aaacaatctg tggaagcaag tacaagtgaa agttcaatca
     2461 ttgaatcttc ttctgaagag gttaaaaata ctttgtggga tagtgagaaa tctactcaac
     2521 tagcaatttt tgtcccacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2581 ctcagaacaa tgtaagtctg tatggtacgc ctttaccaag tgcagttatt gatggaaatt
     2641 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2701 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2761 agcatgtata tttctttggc tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2821 atcaaggcaa tgataaaaac tacatgtact tcaatgaaac tcagaaccaa cagttaaaac
     2881 aaggtttcat tgatattgtg aatggacaaa ctcctcaaac tccagtagta gaagaatcta
     2941 ctcaacaagc aacaactaca acgacacaat ctacaagtag ctcagcaatt tcaagttggg
     3001 aagctgctaa agaaactgta ctaaataata aagctttatg gttgtatgat tctcctgata
     3061 aaaatgcatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3121 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgataatgga aatacaacaa
     3181 tggctggtgg aaaacaattc cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3241 tgactaacaa ttttcaaatg attaaataat tgaaaatatt atttatcaaa gaaaaactga
     3301 ttttgagaaa aat
//
LOCUS       DADOOS010000201         3288 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.207, whole genome shotgun sequence.
ACCESSION   DADOOS010000201 DADOOS010000000
VERSION     DADOOS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.207"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..183
                     /locus_tag="J8C37_002704"
     CDS             <1..183
                     /locus_tag="J8C37_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAZ5306103.1"
                     /translation="DTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPE
                     NEAWLQGALEVFKHEQ"
     gene            173..1390
                     /locus_tag="J8C37_002705"
     CDS             173..1390
                     /locus_tag="J8C37_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAZ5306104.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            1403..>3288
                     /locus_tag="J8C37_002706"
     CDS             1403..>3288
                     /locus_tag="J8C37_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ5306105.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPD"
BASE COUNT         1157 a          559 c          685 g          887 t
ORIGIN      
        1 gatactttcg aagaagaaga accaattgat atggttcatg tcggaaatga catcaagaag
       61 atacgacaag aacagcaagt gctagaaaaa aaacttttag aagctatctc ttccctgcaa
      121 acaacacctg aaaatgaagc gtggttacaa ggtgcgctgg aggtgttcaa acatgagcaa
      181 tgatacacaa ccagaaatcc gtttcccagg atttacagat gattgggaac agaggaagtt
      241 gggggacttc gttgtagatt atgttgaaaa gacaagtgta caaaatcaat ttcctatgct
      301 aacttcatct caacaaaaag ggatagtttt gcaagaagac tattttgcaa atcggcaagt
      361 aactacagaa aataatattg gttactttgt actacctaga ggatatttca cgttccgtag
      421 tcgaagtgat aatgatgtgt ttgtgtttaa ccgtaatgac attattgata gagggattat
      481 aagctatttt tatcctgtat ttactttaaa atctgctgat tccgacttct tcttaagacg
      541 tattaacaat ggaatccaac gacaattatc tattcaagca gagggaacag gtcaacatgt
      601 tctgtctcta aaaaagttta aaaatatagt agcaatgttt cctagtgagg aagaacaaca
      661 aaaaatcggc accttcttca aacaacttga cgacacgatc gctcttcatc agcgtaagtt
      721 agatttgctg aaggaaacga aaaaaggatt cttacaaaaa atgttccctg aaaacggagc
      781 aaaagtgcca gaaatccggt ttcctggttt tacggaagat tgggaagagc gtaagttggg
      841 agaattaggt aaaactcaat cgggaattgg atttcctgat gccgagcaag gtggttctga
      901 aggtactcca ttttttaaag tttccgacat gaataacatt ggtaatgagt atgaaatgag
      961 aaatgcaaat cattatgtat ctaatgagca aattgagcgt aaaaaatgga agccaataaa
     1021 agatgtgcca gcgattatat ttgctaaagt tggcgcagca ataatgctta atagaaaacg
     1081 tttggtcaca agccctttct taatagataa caataccatg gcttatttat ttaataatac
     1141 ttgggatatc tattttggta aaatattgtt tgaaactata aatttgccaa gatactccca
     1201 agtaggggca ttgccttctt ataacagttc tgatattgag aatatcagtg ttaaggttcc
     1261 tgtaaaggat gaacaacaaa aaatcggtac tttcttcaaa cagctagatg atacgatcgc
     1321 tcttcatcaa cgtaagttag atttactaaa agaaacgaaa aaaagcttct tacaaaagat
     1381 gtttgtctag gagggaagaa gaatgaaaat tagtcaccgt gaggaagcag aagtcgaaga
     1441 acagttgatt cgtgtacttg gagaagggca taatcagtgg acgtatcgtc cggatctaaa
     1501 atcagaagaa gacctttggg tcaatttacg ccaaaaaatt atctcaaata atcaagcaga
     1561 actgaatgat tttcctttga ccgataaaga atttgaaacg atcaaaacgg aattactgct
     1621 gcgaacgaaa accccatttg atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg
     1681 gatcacgatt gaacgggaag attcgcaatt gggttctgtc tcacttatcc tttattccaa
     1741 tcaagacatt ggtggaggaa tctctactta tgaagtggtc catcaaattg caaaacgtgg
     1801 aagtaacata gaagcacggg atcgacgttt tgacgtcacc ttattaatca atggattacc
     1861 gattgtccaa atcgagctga aacaagtaac tgcaaaagat ggtgtttatc aggcatttaa
     1921 ccaaatcaaa aaatatgcag aagaaggtat gtttcggaac aatatctttt cgaccttaca
     1981 gttgtttgtg gtttccaatg aacaaaccac tcggtatttt gcaaatgcct taccgaaaga
     2041 cttgcaccct aagtttttat ttagttggcg aacgaaagac aatgaaaaag tagaaaactt
     2101 atatgagttt tgtaaacaag tgttaaatat cccggatgct catcgtttga ttgcggatta
     2161 tacaatcgtc agtgaagatc aagacaataa aaccctcatg gtgttacatc cttatcaagt
     2221 acatgcgatt caagccttgt ttattgccgc aaataaacat caatcagggt atgtgtggca
     2281 tgcgactggt tcaggaaaga cgttgactag ttttgtatct actaagcttt tagcacgaaa
     2341 atcggggatc gatcgaacga tcatgttagt ggaccgcaaa gatttagata atcaaaccac
     2401 cacagagttt acaaaatttg cttcggaatt taatactggg atttcttccg ggaacgccaa
     2461 agcgaatagc ttgatcgttg gaacgggaag tgccaaagaa ttaagtgaaa ccctgttggc
     2521 agatgccaat gcaaatgttg taatcattac aacccgtcaa aaactagatg cggcgttgaa
     2581 atatgcaaag aaacaagaag agaaaaaagg aaccaatcgc ttccaaaaat taatgggcca
     2641 acatatcgtg tttgtagtag atgagtgtca tcgagcgtta agtgctgaaa atatggaaga
     2701 aatcaaaaaa atgtttccga aatccacttg gtttggcttt acaggtacac cgatttttcc
     2761 tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca acccatgatc agtatggaga
     2821 ggtcttgcat acctatacca ttaagaatgc gctagaagat ggctctgtct taggatttca
     2881 agtagagcat gaaaacaccg tggagccgac ttcactagag aataaaatct atcggaaatt
     2941 aaaagaagta gaaacatatg cagagtattc atcagaacag atcaatcgta tgattgatca
     3001 aatggaacct gtcaaaaaag aaagttattt ggacccagct gtttttgaag cagatgaaca
     3061 tatccaaaaa gtcattcata aaatgttccg tccagataat gcctatacaa aatttgattt
     3121 tcgaaatggc aggccaacga aatctgcaat cttaaccacg agttccattg acatggccaa
     3181 aaagtattat cgggccatca aagagatgac gaaagagcct gattggttaa caaaagagtt
     3241 tcccaatcag ctcattcgtg aaggacgaac gatggaggat cctgattt
//
LOCUS       DADOOS010000202         3286 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.208, whole genome shotgun sequence.
ACCESSION   DADOOS010000202 DADOOS010000000
VERSION     DADOOS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.208"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..2177)
                     /locus_tag="J8C37_002707"
     CDS             complement(<1..2177)
                     /locus_tag="J8C37_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306106.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="J8C37_002708"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="J8C37_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          910 a          731 c          528 g         1117 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgaatcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatag
//
LOCUS       DADOOS010000203         3199 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.209, whole genome shotgun sequence.
ACCESSION   DADOOS010000203 DADOOS010000000
VERSION     DADOOS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.209"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            301..585
                     /locus_tag="J8C37_002709"
     CDS             301..585
                     /locus_tag="J8C37_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306107.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="J8C37_002710"
     CDS             588..1004
                     /locus_tag="J8C37_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306108.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="J8C37_002711"
     CDS             complement(1141..1860)
                     /locus_tag="J8C37_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ5306109.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="J8C37_002712"
     CDS             2513..2608
                     /locus_tag="J8C37_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAZ5306110.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="J8C37_002713"
     CDS             complement(2910..3155)
                     /locus_tag="J8C37_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306111.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DADOOS010000204         3159 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.210, whole genome shotgun sequence.
ACCESSION   DADOOS010000204 DADOOS010000000
VERSION     DADOOS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.210"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..103)
                     /locus_tag="J8C37_002714"
     CDS             complement(<1..103)
                     /locus_tag="J8C37_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAZ5306112.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVL"
     gene            261..524
                     /locus_tag="J8C37_002715"
     CDS             261..524
                     /locus_tag="J8C37_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306113.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            548..736
                     /locus_tag="J8C37_002716"
     CDS             548..736
                     /locus_tag="J8C37_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306114.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(819..1004)
                     /locus_tag="J8C37_002717"
     CDS             complement(819..1004)
                     /locus_tag="J8C37_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306115.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1219..1590
                     /locus_tag="J8C37_002718"
     CDS             1219..1590
                     /locus_tag="J8C37_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAZ5306116.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1740..1812)
                     /locus_tag="J8C37_002719"
     tRNA            complement(1740..1812)
                     /locus_tag="J8C37_002719"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1777..1779),aa:Gln,seq:ctg)
     gene            complement(1858..2286)
                     /locus_tag="J8C37_002720"
     CDS             complement(1858..2286)
                     /locus_tag="J8C37_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306117.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2346..3104)
                     /locus_tag="J8C37_002721"
     CDS             complement(2346..3104)
                     /locus_tag="J8C37_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAZ5306118.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1037 a          509 c          594 g         1019 t
ORIGIN      
        1 gtaaaactga aaacttcttt tttgtccatt ttatgtaata gaaaagaatg cttatcataa
       61 taaaaaaagg aatataaaaa acaaattgac cgatcgcgat catactattg cctccgataa
      121 ataaagtcca tgatacaaaa taacactttg tgcgaaatgg cgcaagcagt tttgtggatt
      181 tatccggtaa aatctgtatc gattttgaaa ttagaggaga aacttgctat aatcaaagtg
      241 aaagaaggag tgggattttc atggatcatg tagtgttaga aatcgatttg catttgatca
      301 ataatattcg ggtgatttac tatgttgtaa gtaacagtgt cgaacaacgt gtactgacga
      361 ataaaataaa tggtatttta gcaaagaaag acgttcaccg attcaataat ggtgaaggaa
      421 gttattatag tatcccagta gaaaaaatta tttatacgac agtcaaagtc agagaagatc
      481 tagataccaa aaaagcatat gaacctattt ttacaactta ttgattgaca gcagatggag
      541 gaaaaaaatg aatccatatg aacaacttat tcaagatttg atcgatggaa aaatcgaaaa
      601 gattgatgta aaaagagaag aattaatggc ttttcgagaa gcgtggctga agttggagaa
      661 tcgtaaatat gtggtgggaa aagcctcact gaacggcaat gtgacttatc attacgatcc
      721 gacacgttta ttttagtatg aagtattcag aaaagaacca aaggaggacg atgcagcagc
      781 attgtcctcc tttgatttta caaaagtgaa agttttactt atttgaatag atttttaaat
      841 ttgtttttga cactgtcgat atttacatct tttaaaagat tttgggcttt ctctttataa
      901 ggtcctaatt gttctttatg ttcttcgata aatcgttttg cgccatcgaa attgccgttc
      961 ttaatcattt ccttgacttt ttgaaataat tcttcttgat tcattttgaa cacatccttt
     1021 gctatcaaca tgatttttta catggacaaa acgagaaaaa ctgatttatc cacacgtcaa
     1081 taatatcaaa taacgatcat ttttactaaa aaaacactcg attctcgctt ctaaagtagg
     1141 agaaaaatct ccaacttggg acggatggaa tttctttaac tgacccatat actgaatata
     1201 taacaaggag gtagaaagat gaaagtgatt aaatatttga ttggactggc ggcgattagt
     1261 ggtattttgc tatttggact gaaatattat actgaaggaa gttatggaga aatccctggt
     1321 atacttgatc agctaaatcc attagttgaa aaaggagagg tctatgtaaa gacacaaaaa
     1381 ccagaggaag taaatgaata tggtacagca aggtatgtgc agaaagctgc tgatgccaat
     1441 gggaaagaac gaacggttga atttaatggt ctttctgtgt taaaagaaaa tcattatttg
     1501 aaaattagca ataaaggagc acacatagaa acgtatgagg aagttcctag agaaaaagtt
     1561 ccggagaaag cattagcaat tattgggtaa atcagttttt gtattaagaa tacgcccgat
     1621 tttattattc ataatttaat tatgtaaact aaatacaagt agtaaaaata atacaataat
     1681 ccccctgcaa aaagattgtt cttttacagg gggttcattt tttcagcaag cacatcaaat
     1741 tgggatggca gggttcgaac ctacaattgc ttgattcaga gtcaagtgcc ttaccagttt
     1801 ggctacatcc catttattca acaaaataat catagcactt tcctaaaaaa acgcaagtta
     1861 tttttcttga tcggatagtt gttgctttac tcgtaaaagg cggcgtacta gtttcttttc
     1921 taatccagca ggaagggcaa tcagttctgc tactgacaag acggtgtcca ataactgata
     1981 ttctttgaca ggttcattca taatatgata gatgtttgct aaacgttcaa tatattcaaa
     2041 gcgagtatac tctgttcgag caattagaaa tttcaataaa tcggtggctt tctcgtactc
     2101 ttgatctgcg taataaacaa gagattgacg atacaatttg atttccagtt cattgagggt
     2161 agcaggtctg cgacgctcgt tccactcatt aatagagact ttaccttgaa catttccctt
     2221 gcagtcacgg acaaaaataa tcggaatttc atttggatcc atttttttat gtgtgaattt
     2281 ttccattatc tacgctccct tcaaaggttt ggtatcttga gtataaggga tttttgtttg
     2341 aattactagc ttttctccaa agttagcatc tgtccaccca ttgcagtgaa aatcgccgaa
     2401 aaatcgttga tcggtactgc tcgatttttt acaccatagg gatcgttgac ataaatattt
     2461 tctttgtcat atccagtgac tacacaagca tgaacaagag gagtgactct tacttctcca
     2521 ttatttgtgg tccaagtcat aaaatcagtt tcatctggga cttcaaaatt gatagtcaca
     2581 gaagtccaga caggatttcc tttagatagt gcttttttga tatcatcgag ttgagcatcg
     2641 gtttttggaa aagtgtgata atccgtttgg acgatttgct gagcaaggga ggcaactggg
     2701 ggaacataga cgaccatagc ccagtctccg ccactgatat caccaacaaa tccttcatta
     2761 ggatcgccat gagtcccatc tgcattgaaa acagggacgt aatccaattg ctcagctaac
     2821 tggtttttag ttgtttcata accataaaaa gataaaagca tagaaagcga agtgacttca
     2881 cagccatttt ctaaagaagg agaatcaaac tggctctcta atggaacatc tagtaaaact
     2941 tctgctttct cttttgtctt tttcgttttt tgtgttgaac ttttttgtgc agtgttcatt
     3001 gtatgttctg gagaatggat atgtaggtaa taacttgtac cgcttaataa aagagcagct
     3061 gtgcccccta gaaacaatag ttttttgaac gtttttttct tcatttgaaa tctccctttt
     3121 cagctgttta tctttcctat tataaatgta tttttattg
//
LOCUS       DADOOS010000205         3156 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.211, whole genome shotgun sequence.
ACCESSION   DADOOS010000205 DADOOS010000000
VERSION     DADOOS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.211"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..522
                     /locus_tag="J8C37_002722"
     CDS             <1..522
                     /locus_tag="J8C37_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAZ5306119.1"
                     /translation="KILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSHIDIDYV
                     FLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGLMAIRFL
                     ADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSELAKKVKV
                     TFLTDPRWVRIDK"
     gene            657..1235
                     /locus_tag="J8C37_002723"
     CDS             657..1235
                     /locus_tag="J8C37_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306120.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1181..1699
                     /locus_tag="J8C37_002724"
     CDS             1181..1699
                     /locus_tag="J8C37_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306121.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1707..2747
                     /locus_tag="J8C37_002725"
     CDS             1707..2747
                     /locus_tag="J8C37_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAZ5306122.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            2996..>3156
                     /locus_tag="J8C37_002726"
     CDS             2996..>3156
                     /locus_tag="J8C37_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5306123.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1122 a          351 c          633 g         1050 t
ORIGIN      
        1 aaaattctac tgattgcacc aggaaaaagt tctgtagatg aggtagaaag aataaaagag
       61 tttgttacca aagaagaagt cgtaataatt agtgtaaatt ttgaatattc tcatatagat
      121 atagattacg tatttttaag taatttaaga cgttttcatg aattaccgaa agaaagcaga
      181 actaaatgta ttgttacatc aaatatacaa gcatcggatg tatatttacg gacgagttat
      241 aaaaagttgt tatctgaaag ggaagttgta agagacaatg caggattaat ggcaattaga
      301 tttttagccg atatgaatgt ggatgaaata ttcttagctg gatttgatgg atattcacat
      361 aatgaagatg agaattatgg tgagcaatcc atggaaatta taacaaaatt tgcattatta
      421 gatgcaatga acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa agtaaaggtg
      481 acatttttaa cggatccacg ttgggttcga attgataagt agtagtaata atgtttttaa
      541 aattttgata aagagatagt gagagaataa aaatgggtaa agtcttaaat aaaaaagaag
      601 aaaaatattt aagggacgat ggggcgagat gaaactctaa ttttgaactt ttaagaatca
      661 tttctatatt gtgtattgtt gcttaccatg ctaatttgcg tttaaattat aatgcagacg
      721 gaattatagt acatggttca ttaactgcta accagatttt cttgaattat attggtattt
      781 ggggtcagct aggagttaat tgttttatat ttatttcggc atatttttta agtgagaaaa
      841 gaaacattca ctttgaaaat gttttgggaa ttgtattaga atgtattttt tatagtgtat
      901 tagtttatgg aattctttgt gcatgtggaa tagaaacatt ttcattgaac agctttgtaa
      961 aagaaatatt atcatttgct ctaggtagat attggtttat tacatcctat ttagcattgt
     1021 atattctaag tccgtattta aatatcatgc ttaacaatct ggaaaaactt cagttgcaaa
     1081 aaataagtat gttattgttt ataggtgtat gctgttatcg tatgttattt aaaacatctt
     1141 tgtttgggga ttgtgctgtt ttaattgcac tatatgtcat atgttataca ctgaaaaaaa
     1201 cgataataat atttttgaga gacacagaat cataggaaca attgtatgta taacagttat
     1261 accacttctc atatccatag gaatactttt caaatttata ccaaatgcaa tggttaacca
     1321 gatggttcat attggttctc cattgatggt tatagcaagt atatttttat tttatgtatt
     1381 taaaaatttt tcattttaca gtaagtatat aaataaactt gcgagaacca gtttaggggt
     1441 atatatttta catgaaaatc attatttcat tgatggtatg aagtctagag gcttaatatg
     1501 ggatgggata tttggattag gcaaaaggtt tttagatagg gatttttgga tttggtttat
     1561 aattgcagtt gtagttgtgt gcataatttg catggctatc gatgttgtaa gagaaaaatt
     1621 tgaaagacag atttttaaaa ttaagagtgt aaagaacctg tgtaaactta tgaatcatgt
     1681 tataaatata tacccataat taataaatga ggagaaaaga agtgaaagtt atatcattcg
     1741 gagaattatt attgagactt gcagcacccg gttattcaaa gttatttcaa agagatagtt
     1801 tggagactac attctgcgga ggggaagcta atgtagcggt atccttagca atatttggat
     1861 atcaatcaga atttataaca aagcttccgg attcagatgt tggatatgcg gcagctaatg
     1921 ctttgcgtta ttttggagta aatgttgata atattatatt tggagaggga cgcatgggac
     1981 tgtattattt agaaaaagga gcagctcaga gaccatctaa agtaatttat gacagaaaat
     2041 tttctgcaat ttcgttagct gatagtgatg aatttaagtg gaaggagatc ctaaacgatg
     2101 caaattggtt tcattggaca gggataaatc cagcattgtc tgagaatctc tttgagacgt
     2161 ccttagatgc atgtcaggct gccaaaaaaa gaggtataac aatttcttgt gatttaaatt
     2221 atcgatccaa gctttggaca tcagaggaag caaaagaaaa gatggaacag atactaccat
     2281 tggttgatat ttgtatttgt aatgaagaag atgcagaaaa agtgctagga attaaagctg
     2341 agcatacaaa tattaatagt ggagaattaa ataaagatgg ctatgtgcag gtggctaggg
     2401 agattcacaa gaagtttgga tgtaggtatg tagctacaac tctgcgtaga agttattcgg
     2461 caagtagaaa tggttggagt gcaatgttat acgaagcaga aaaagataaa gcgtattttt
     2521 ccacagaata caacatacaa attgttgatc gcgttggtgg tggagacagc ttttctgctg
     2581 gtataatata tgcgttatgt gaaaatatgt ccggacaaaa ggctattgaa tttgcgactg
     2641 catctagttg tttaaaacat actatagaag gtgattttaa tcgaactaca attgaggatg
     2701 tatataattt aatgaatagc ggtggaaatg gtcgcgtgtt gcgttagtca acatataaag
     2761 ataaatcaca gtaattttta tggtgattat ctgtcttagt agtgattaga ataacgtgaa
     2821 aataaattgt tgagcgttta tactgttttg aaaaataaat ttaacatatt gaaaaataca
     2881 aatatatacg tatattataa agcgccgatt gtaaaattaa gctagacaac taaaacagtc
     2941 atttgtgcta cactcaaaat agttccgacc aaagaattat aaggagtgag tgcaaataac
     3001 ctatacctat cttacaacgg atgaacttgt aataatagag tcttatttga aaattaacca
     3061 atctgttgct aaaactgcgc attgcttgaa tcgttcaagt caaacgatcc ataaagtata
     3121 tctgttcttc aagcaaagaa aatcagcctt agaata
//
LOCUS       DADOOS010000206         3123 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.212, whole genome shotgun sequence.
ACCESSION   DADOOS010000206 DADOOS010000000
VERSION     DADOOS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.212"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            93..848
                     /gene="fabI"
                     /locus_tag="J8C37_002727"
     CDS             93..848
                     /gene="fabI"
                     /locus_tag="J8C37_002727"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAZ5306124.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(958..1971)
                     /locus_tag="J8C37_002728"
     CDS             complement(958..1971)
                     /locus_tag="J8C37_002728"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAZ5306125.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2136..3056
                     /locus_tag="J8C37_002729"
     CDS             2136..3056
                     /locus_tag="J8C37_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAZ5306126.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          913 a          626 c          593 g          991 t
ORIGIN      
        1 ctaataatta agaaatcaag aagttatttt agctattatt ctctattttt ttacgactat
       61 caaattttta tcagaaacaa aggagagtca ttatgtcatt tctagaagga aaaaaaatcg
      121 tcattatggg cgtcgctaac aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga
      181 aacaaggggc agaaattatc tatacttacc aaaatgaacg aatgaaaaaa tcattagtca
      241 aattggtcgg cgaagaagca ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg
      301 aaaaagcaat gaatgccatc ggtgaatatg ctggggagat ccacggcctt gtccatgcag
      361 ttgcttacgc caacaaagaa gaattgtccg gaaatgtgtc tgatatttcc agagacggct
      421 atcaattagc tcaagatatc agcagctact ctttaattgc tgtatcaaaa tacgcacaac
      481 cgtttcttgc accaaacagc gggatcgtta gcatgactta tctaggttca gaacgcgcaa
      541 tcccgaatta taacatgatg ggtattgcga aagcttctct tgaagctgct atccgttatt
      601 tggcagctga gttctcgcct aaaggaattc gggtcaatgg aatctcagct ggggcaatca
      661 aaacattagc tgtcacaggt gttaaagact atcaaaaact gatccagtta tctgaatctc
      721 gtacgcctga tcagcaaggt gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa
      781 tcagcccact ttccagcgga atcatcggtg atattatcta cgttgacaaa ggtgtccatc
      841 tttcataaac ttttgttgca ttctttaaag taaaacatat aaaaatcagg aaaaaacttt
      901 ttaaagtctc ttcctgattt tttcttttta cgtttttttc aactcattac tttgaaatta
      961 ttccacgact tcaatccagc cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa
     1021 ttcatcatac aaccgttttg ttactggtcc tacttccgtt tcactataaa atacatgaag
     1081 attgttattg tgataaatcc caccgattgg agaaataaca gccgctgtac cacatgcacc
     1141 agcttctgca aattgatcta gatcatcaat gaaaatatct ccttcaattg cttgcagtcc
     1201 tagtcgttct tcagctaact gcaacaagga atatttcgtg atacttggta agatagaggg
     1261 cgattgcgga gtgatgaatt ttccgtcttt tgtaattcca aagaaatttg ctgcacctac
     1321 ttcttcgatt ttcgtatgtg tggctggatc aagataaaca caatctgagt aatccagttc
     1381 ttttgcttct gttcctggaa gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc
     1441 agtcccctta ggagcagcac gatcgtaatc agatacaata aaattcgttg gttttagtcc
     1501 acctttgaaa tacgctccta ctggcgtaca gaagacgaca aataaatatt ctggagctgg
     1561 atggacacca atgttttcac cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc
     1621 tgttccatag ggcggcacaa aatcttcatt tgcttttacc acttgcttga cagcatcaac
     1681 gaacatcttt tcagggacca ttggcatcag caatcgtcta gcactttgat tcatccgtcg
     1741 actattttgt tcaggtcgaa agagattgac cttgccgtct tttcgacgat aagcttttag
     1801 accttcaaaa cattgctgac cataatgaag cgctggagag ccttcatgaa tcgtcaggta
     1861 attatcttct gtcaattctc cttcttccca ttgtccgtct ttccatttgg caataaaacg
     1921 ccaaggagtt ttgatataag aaaaacctag attgttccaa tcgatttgca tatacttacc
     1981 accttcgtta ttttctccac attttatcat tatccatatt atttgtcatt ttattttcag
     2041 cttttttcct tcattatagt tatttttgct atttcctttt ttgaaaactt cttggtacaa
     2101 tacagaaaga atcaaatttt agaaaagagg attttatgtt ttcattatta gcaactgccg
     2161 gtacgacaga ctcttctggc gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt
     2221 ttcagcggtt ttgggacggg attgattggg acgccatttt ggctgccttt atccaaaaaa
     2281 gtttatatct gatttttttg atcgtccttt tttggattct tcaccgtgtg ggaaaatatc
     2341 tgattgataa gtcttttaat cagtattcta aaaaagccat tctaaacgag agccgcttaa
     2401 agactttaca tagtctttta aatactattt ttcattatac ccttggtttc ttttttatct
     2461 atgcaatttt atctgctatc ggtgtgccga tcggatcact tttggctggt gccggaattg
     2521 caggtgtggc aatcggtctt ggtgcccaag ggttcatgag tgatgttatt accggatttt
     2581 ttattatcat ggaacaacag atggatgttg gggactacat caagctagca aacttaacga
     2641 ttgaaggaac cgtcgtatct gttggtatcc gtacccttca gctgaaagca acggatggta
     2701 ctgtccattt catcccgaat cgaaacatca ccacgatcag caatctctct cgtgcaaata
     2761 tgcaagtact tttagatatt cggattgttc ctgaagaagg atatgacaaa atctatgaga
     2821 tcattgatcg agtaaatcgt acattagctg aaaaatacca agaagagctc caaactgaac
     2881 cttctatctt tggattagtg gatcttggaa acagcaattt tgcgattcgt acggtctgct
     2941 acgcattgaa cggaaaacaa tatgccttaa aagaagagtt cttgagctcc tatgtcaaag
     3001 aattgacagc ttctggtttc acgattccga acagtccaat tgctgtcgga aaataatttg
     3061 gacaaacgat gcaacgaatc tcactaaaaa gagcgtggga caaaagtaaa gaacactttt
     3121 gtc
//
LOCUS       DADOOS010000207         3026 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.213, whole genome shotgun sequence.
ACCESSION   DADOOS010000207 DADOOS010000000
VERSION     DADOOS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.213"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            758..>3026
                     /gene="esp"
                     /locus_tag="J8C37_002730"
     CDS             758..>3026
                     /gene="esp"
                     /locus_tag="J8C37_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAZ5306127.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1107 a          442 c          590 g          887 t
ORIGIN      
        1 gatgaattta tggattatca aaaattggat cagttaattg aaagagaaag ctgggaagat
       61 tattacgtgg gtctaacttt ttcagaaaag ttaaacaact acaagttaat agattataag
      121 tatttgagtg aaagcaatta attattcatg tagagtacta gacactttaa atatggacaa
      181 ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg tcattttttg
      241 tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta agattctagg
      301 aatcattcag aaatgctgtg aaaccgatac tttataatta taaagaaggt gatttcaaat
      361 tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt cagtaatgcg
      421 ggataaggct ccttattccg ttatttgaat actctgaagt ttaatctaag aaagggtaat
      481 attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga atacatacat
      541 atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc gaaaaaaaca
      601 tgaagaaatg tacttttttt gttgtaatgt tcatgaaatt ctctaagtaa attagttaac
      661 ataaggttag taagattcct attctgtgaa taatacgtat cagatttatc ttttattctt
      721 actaggaaag taattattaa gggagttgat ttgaataatg gttagcaaga ataataagag
      781 agtatttcta gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa aattaagtgt
      841 tggggttgca tctgttttga ttggtgttgg tcttgtcttg ggaacaactg aactagttaa
      901 agcacaagat gaaatttctc caagcagccc acttgaaaca gcgataagct ccgtacaagt
      961 aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat cctggttata cgaaaaatta
     1021 taacttttcg gatttacaat ttagtcctca agaactaact ggagacacac taaaaggcaa
     1081 tacaattgga tttgaagttt acggaaaaca taatattgca gcatcaacta aaaactggga
     1141 aatccgtctt caattagatg aacgattagc taaatatgta gaaaaaattc aggtcgatcc
     1201 aaagaaagga attggatctt ctagacggac ctttgtaaga attaatgatt cgctgggtag
     1261 acctacaaat atttggaagg tcaattatat tcgtgcaagt gatggattat ttgctggggc
     1321 agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt acgtttgaaa agagcctaga
     1381 tgaaattttt aaagagatag gcatagacaa tctcaagacc gatcgtttaa tgtatcgaat
     1441 ctacttggta agtcatcaag acgatgataa aattgtaccg ggtattgata gtacgggcta
     1501 ctttttaaca gattcagatg atttctataa tagcttagat gtatctgaaa ataatcctga
     1561 ccagtttaaa catggatcag taagtgcaaa atatgagaaa cctaatactc aaacaaaaga
     1621 tggttcagga tccacaggtg ctaatggagc tataatctta gatcataagt taacgaaaaa
     1681 ttataatttc tcatattcat cttccgcaaa gggcacgcct tggtatgcta attacaaaat
     1741 tgatgaacga ttagttccat atgtagcggg tatacagatg cacatggttc aggctgacaa
     1801 agtaacatat gatgtttcgt ttgaatccgg aacaaaagta gctgatttag caatcgagcg
     1861 acgggaaggt catgaaaatt atggcatagg ttcaataact aataatgatt taactaaact
     1921 tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt cgatatgttt tacaattgac
     1981 taagccatta gatgaaatct tagaagatat gaaagcaaca gcacaagttg aagaaaataa
     2041 accatttggt gaagatttca tctttgattc ttggttgtcg gatacgaata aaaaattaat
     2101 ccagaacact tatggaacag gttattatta tttgcaagat attgatggtg atggaaaccc
     2161 tgacgataaa gaagagagcg gagacacgaa tccatatatc gggaaacctg aattagaaga
     2221 agtatatgat gttgacacaa cagttaaggg gaaagtattc atccacgagt tagcgggaac
     2281 aggtcacaaa gcccaacttg ttgataaaga aggtactgta ttagcagaaa aaactatcgc
     2341 tccaaatgaa aaagatgggg ctccaatttc agatactgta gaatttgaat ttacgggtgt
     2401 agattcaagt aaattaatcg cgaaagatga attaaaaatc caaatcgttt ctccaggttt
     2461 tgataaacca gaagaaggtt caaccgttat taaggaatca ccaaaagcgg ttgataaaca
     2521 aaccgtggta gttggattta aaccagatgc taaagaatca attcggaata ataaaaactt
     2581 acctgaagat gcagagtatt catggaaaac agagcctgat acttctaacg ttactgatag
     2641 tacgaaaggt attgtaactg ttaagatcgg aaatcgaact ttcgacgtgg atgtagagtt
     2701 tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta taacaacaac
     2761 tccagaaacg acagttcaaa gtggaaaacc tacatttgat aaaccagatg ttcctttagc
     2821 taaagatgcc ttttcaattt cagatgtcta taataagtac ttcggtaatg caagtgttga
     2881 tgcaaacact ggtattgtta cattcactcc agctaaaggt gtaggagaat cggagccgat
     2941 tactggaaca attcctatta aaattgttta ccaagatggt tctgtaggca cgaccgattt
     3001 agcagtaact gtaagtaaag atattt
//
LOCUS       DADOOS010000208         3024 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.214, whole genome shotgun sequence.
ACCESSION   DADOOS010000208 DADOOS010000000
VERSION     DADOOS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.214"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1243
                     /locus_tag="J8C37_002731"
     CDS             <1..1243
                     /locus_tag="J8C37_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5306128.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="J8C37_002732"
     CDS             1256..1888
                     /locus_tag="J8C37_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAZ5306129.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="J8C37_002733"
     CDS             2093..2320
                     /locus_tag="J8C37_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAZ5306130.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="J8C37_002734"
     CDS             2389..2655
                     /locus_tag="J8C37_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAZ5306131.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="J8C37_002735"
     CDS             2645..2914
                     /locus_tag="J8C37_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAZ5306132.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DADOOS010000209         3016 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.215, whole genome shotgun sequence.
ACCESSION   DADOOS010000209 DADOOS010000000
VERSION     DADOOS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.215"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            339..422
                     /locus_tag="J8C37_002736"
     tRNA            339..422
                     /locus_tag="J8C37_002736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:373..375,aa:Leu,seq:cag)
     gene            complement(746..1384)
                     /locus_tag="J8C37_002737"
     CDS             complement(746..1384)
                     /locus_tag="J8C37_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5306133.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            1719..2153
                     /locus_tag="J8C37_002738"
     CDS             1719..2153
                     /locus_tag="J8C37_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAZ5306134.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            2176..2496
                     /locus_tag="J8C37_002739"
     CDS             2176..2496
                     /locus_tag="J8C37_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306135.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            2637..2828
                     /locus_tag="J8C37_002740"
     CDS             2637..2828
                     /locus_tag="J8C37_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306136.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2899..>3016)
                     /locus_tag="J8C37_002741"
     CDS             complement(2899..>3016)
                     /locus_tag="J8C37_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAZ5306137.1"
                     /translation="FSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
BASE COUNT         1097 a          464 c          637 g          818 t
ORIGIN      
        1 aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac agaagaaata
       61 atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa agaaatgaag
      121 aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa tcgatcatat
      181 ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg gatgaattat
      241 ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa tatgttcttg
      301 acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg gaattggcag
      361 acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc ttcttagccg
      421 cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag aaaataacat
      481 attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa ataaatcaaa
      541 aaaaatttgt gtgaaggtgg gacagtttaa aacctaatag attgattcaa atagtagatt
      601 tatagattct gatacaagaa atactggcaa acgatagtta aggaataaag actaaactta
      661 aactcaaaaa gaggtcatgc gaaagaataa atcgcatgac ctcttactat attttgaatg
      721 tgtttaaacg ttcaaataat gaatcttagt accaaccgtt tgcttcccag aatgctttcg
      781 ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt tcttggtttg
      841 cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacggccg atgtaacgtc
      901 cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct tttgcagagc
      961 ttgtacttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt gtttcagcaa
     1021 caggcgctgc ttgtgtttcc gtttgaaccg tttcttgtac aacaggttgt tcagcttgaa
     1081 caggttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca gctgcttgtg
     1141 cagagccttc tgttgggatt gttaattgtt gtcctgagta gatcaggtta atatcagaaa
     1201 ttgaatttga ttctgcaatg gcattgatca aggagttatc accaacgtat ttttgagaaa
     1261 tcgttgatag agtatcacct gattgaacgg tataagcttc gtccgcatga gcagtcgttc
     1321 ccatgaagaa tgcagcgccg gcagccaaag ttgttccaaa aagtaaagtt ttaagtgatg
     1381 tcatgaaaaa tctcctttat tcgttaaaaa gttttatttg ttgtgttcaa cgaggaatac
     1441 tttaacacgt gaaaatattt cataggtaaa agaagcatga caatttatga caaatccacg
     1501 ttttttgtaa cagaatgatg ttcgctcgat gtgaaaaatg atgaggaatt tcacataatg
     1561 cagaaataat gggattagaa caggtgttct gatgagaaaa gaaatgtata aataacttga
     1621 aagaaattgc ttttgttcat cattttattt acatattgcg tatatttttt gtatttttgc
     1681 ttgagatgtt acaataaaag aaaaaggagg cggttccaat gagtaaaaga taccagaaca
     1741 tcatggtagc agttgacgga tcaagacaat cgatcaaagc gttcgctgaa gcccttgact
     1801 tagcaaagga caatgaagcc cacttattta tcgtgtcaat catcaacaaa gttgagctga
     1861 cccatagtgc ctatgccttt tcaaaaatct atgcagatga aaaacaaaga acagaagtag
     1921 ccatgctgaa aaaaatcaat gatgccaaag agtacggcat tgctgagatc cacgcaatag
     1981 ttgaaactgg agatccaaga aatttgattg ccaatgtcat ccctggacaa gaaaatattg
     2041 atttgatcgt tatgggagcg acaggaaaag gtgctataca acaagcatta gttggatcga
     2101 ccgcttctta tgtcgtatca catgcccctt gcagtgtgtt ggtcgtgaag taatgagata
     2161 agggaggaat agaaaatggt tcgtcctcaa gaagtccgtg cgccaaaaga aaaaatagaa
     2221 attttagcaa ttattgaaga cggcacacaa acaaagaagg gatactcgat cgctttagta
     2281 aagtggaaag gaaaaaaagg tgttgctctg agatgggatg gagataacca acaagataaa
     2341 ggattccctg tcacagctaa cggttatcat ccagcgtggt ttgttctgcc tgataaattt
     2401 atggaattat acagctatga ttatgcaaga gccgcagcat caatcaaagc attagataaa
     2461 ttaacagaag agcagaatga aatggaggaa aaataaaaaa gacgaaccaa tcagccttta
     2521 gaaaactgat tagttcgagg agtaaaaatg aaaaaaggtt atttgggatg aaactagaat
     2581 aatgcgtata acttaaataa aacttaaagc aataaaatgg caagagggtg aggcaaatgg
     2641 aagcaggaaa agtattacag gaagtaaaag cactatgtgc agataaaaaa tatgaagaag
     2701 cgaaaagcct gatcgaaaag aacaaagaaa atttggaaga aaagtacccg atcgcacaag
     2761 aatttataga attaaaacaa ggaacgatcc ttcaccgatt caaacatttt tttggtataa
     2821 cagaatagaa aaaacgatta aagatcggca gaaatttgct gcatctttaa tcgttttctt
     2881 tttaaacatg cgagggattc aattctgatt cttatataaa aaatagatcc aaataataag
     2941 aaaaattact gaaagcgtca attcgatgat gtgtgttatt aatacagacg ttgtttccca
     3001 atgcctaaaa gaaaaa
//
LOCUS       DADOOS010000210         3012 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.216, whole genome shotgun sequence.
ACCESSION   DADOOS010000210 DADOOS010000000
VERSION     DADOOS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.216"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            177..359
                     /locus_tag="J8C37_002742"
     CDS             177..359
                     /locus_tag="J8C37_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAZ5306138.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="J8C37_002743"
     CDS             462..746
                     /locus_tag="J8C37_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306139.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="J8C37_002744"
     CDS             762..998
                     /locus_tag="J8C37_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306140.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="J8C37_002745"
     CDS             1020..1274
                     /locus_tag="J8C37_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306141.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="J8C37_002746"
     CDS             1356..1541
                     /locus_tag="J8C37_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAZ5306142.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="J8C37_002747"
     CDS             1608..2003
                     /locus_tag="J8C37_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAZ5306143.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="J8C37_002748"
     CDS             1993..2370
                     /locus_tag="J8C37_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306144.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="J8C37_002749"
     CDS             2371..2766
                     /locus_tag="J8C37_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306145.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DADOOS010000211         2992 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.217, whole genome shotgun sequence.
ACCESSION   DADOOS010000211 DADOOS010000000
VERSION     DADOOS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.217"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(106..1053)
                     /locus_tag="J8C37_002750"
     CDS             complement(106..1053)
                     /locus_tag="J8C37_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ5306146.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="J8C37_002751"
     CDS             1299..1763
                     /locus_tag="J8C37_002751"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAZ5306147.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="J8C37_002752"
     CDS             complement(1825..2868)
                     /locus_tag="J8C37_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAZ5306148.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          989 a          593 c          571 g          839 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DADOOS010000212         2977 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.218, whole genome shotgun sequence.
ACCESSION   DADOOS010000212 DADOOS010000000
VERSION     DADOOS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.218"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1128)
                     /locus_tag="J8C37_002753"
     CDS             complement(<1..1128)
                     /locus_tag="J8C37_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ5306149.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMD"
     gene            complement(1258..2634)
                     /locus_tag="J8C37_002754"
     CDS             complement(1258..2634)
                     /locus_tag="J8C37_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAZ5306150.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2634..>2977)
                     /locus_tag="J8C37_002755"
     CDS             complement(2634..>2977)
                     /locus_tag="J8C37_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAZ5306151.1"
                     /translation="KSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAP
                     IILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKL
                     KKVNKMNLEVE"
BASE COUNT          904 a          572 c          417 g         1084 t
ORIGIN      
        1 atccataaaa tggactttgt ctacagtctg cgcattctca ttagagaaca ctcccttttt
       61 tgtgactttt ctgaagctag agtgaatact ttcaactgca ttagtagtgt acatgatttt
      121 tctaatggcg ctgccataat caaagagttg ttcaacatga gaaaagtttc gtttgcatac
      181 atctactgca ccagaataat gagaccaccg attttgaaaa ctgtcgaagg cagcgtgtgc
      241 agcgtttaaa gaagaagcac tgtagaattt tttcatgtct cgacagactt ccttataatc
      301 cttacttggg aggtatcgta acgcatttcg aacaagatga acaatacagc gctgcacaat
      361 gacagaagga aaaattgctt ttgcgccttc tttgagaccg gaaacaccgt ccatcgaaat
      421 gaaaaaaaca tcttctacac cacctacttt cagttcatca aatacttgca tccagcgatt
      481 tttagattct gtttgattga gccataggcc taaaatctcc ttgtttcctt taagatcata
      541 gccaagaatg gtgtacacag catattcttt agcttcatag ttttcgcgta aagtgacgta
      601 catacaatca acgaatagaa atgcataaca cttggctaat ggccgagctt gccattcttc
      661 caattcagga aggacggcat ccgtgatatc agaaatcatt tcgtgggaaa tatcaaagcc
      721 gtaaatatct tcaacggttg cggcgatatc gcattgactc attcctcgtg catacatgga
      781 aagaacctta ccttcgatgt cggagacatc tcgttttcta ttaggaatca actcaggttc
      841 aaaagaagcc tcccggtctc tgggaacatc aatgtttact ttaccaaagc tcgttttaag
      901 cgtcttattg ccataaccgt ttcgtcgatt gtcatgtttc tttggctctt tagaatgggc
      961 gtcataccct aaatggttat tcaattctcc ttgaagcatt ttttcaaata agggaccaaa
     1021 cacatctttt aaaacatctt gcatatcctc tacagattcg ggttgataag tattcaaaat
     1081 ggattcagct aacttctcag catcaggatt tcttttcttt ctagccatcg tgtaatcacc
     1141 tcttgatctt attttagaaa aataaaaacc gtgaagcaac cacccgccta ggattgatgg
     1201 ttacttacac ggtttatatt acactctcat ttttggtgct aaaaagcacc gaaaatttta
     1261 attattgtca attaataatt cttttggatt ccttcttaat atattgaatg aagaaataat
     1321 caatgataca agaagtacta aactcataaa tataactaca taaatcatga atttaggtaa
     1381 tacattgata tctaaacttg ataatgtctt gttaaatcct tcagcttctg ccccaccacc
     1441 taattggcta gatgcagatt gtttagctat ttgtttagct atatcaccag taaccttatt
     1501 caatatatta ttcccaagct tgtcagctgt atattgagct aagaaatagg aaccaacgaa
     1561 tgcggggatt gatataaata ccatctcaat taggaattga ccaaaaattt ctaattttga
     1621 tatacctaat gatagtaata ctgctatttc tttctttcta gcattcatcc ataagaataa
     1681 taataatgaa actacaactc ctgcaaatat taatgagcca aaaaataatt tatttgaaat
     1741 tgagtaaata cctgatatag attgttgtaa tgcagggtaa tttgatgaac ttttaatcaa
     1801 attatattct ctccaattta tatctaattt cccaagatct tttattacac tatcaagatt
     1861 cttatcacct tttacaaaga atgttgcatc ttggtataca gctgtatctt cggtattacc
     1921 ataaacttta gcagctgtat gaatatcagt gattaatgta ttttcgtata gttcttgtgc
     1981 tgcacttact ccaccgctat tatgcccatc gaatagacct ttaatttcta cttctactgt
     2041 ttcatctgca cctttctcat tatcagcatc aaataaatta gattttattt ttatcttatc
     2101 tccaacttta aggttatttt tttcagctaa atctttatgc attaagattt tatttttatc
     2161 ttcattttct aaatgctttc cttctactaa tttatatgct tctgatacaa attttgtttc
     2221 ttttgttgag tcgttaaccc cagttaacat aactgttctt ttaaaattct ttgctctttc
     2281 aggtgattga ttcgcaagag tctcttgagt ttcaataata tcataatcaa ctaaatctgc
     2341 aacactgttt attcttttta cataggagtc tatactatct gtttgagata tctttttaat
     2401 atcttcacct tttacatttc ccccacctct aggtgttcct ggatttactt gtctatttat
     2461 ctccatagaa aaactatttg ttatattagc aaatgtttct tttgaagctc tgtccgtagc
     2521 atctttaata gataaactaa tgagacttaa agttgacata gataaaataa ctaatatgat
     2581 aattaatgat tttaaacttt ttctagttac ataagcaaat gcatttttta tcattattca
     2641 acctccaaat tcattttatt tacttttttc aactttttac cacttagttc taaaatgata
     2701 tccgcagaat ctgctacttc cttactatgt gttacaacta ttacacattt atttctatct
     2761 ttagctaatg tctttaatat attaattatt tctccagcag taacactgtc tagattacca
     2821 gtaggctcat cagctagtat tattggagca tctgatacca atgctctagc aatagctact
     2881 ctttgttgct gtccaccaga taatttcata acatttcttt ttatttgttt tttatctaaa
     2941 cctagttcaa acaagatact ttcatctgct gatttat
//
LOCUS       DADOOS010000213         2937 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.219, whole genome shotgun sequence.
ACCESSION   DADOOS010000213 DADOOS010000000
VERSION     DADOOS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.219"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            191..547
                     /locus_tag="J8C37_002756"
     CDS             191..547
                     /locus_tag="J8C37_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ5306152.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            557..1456
                     /locus_tag="J8C37_002757"
     CDS             557..1456
                     /locus_tag="J8C37_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAZ5306153.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1853..2932)
                     /locus_tag="J8C37_002758"
     CDS             complement(1853..2932)
                     /locus_tag="J8C37_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5306154.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          793 a          649 c          688 g          807 t
ORIGIN      
        1 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
       61 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
      121 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
      181 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
      241 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
      301 ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca
      361 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
      421 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
      481 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
      541 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
      601 taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
      661 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
      721 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
      781 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
      841 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
      901 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
      961 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     1021 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     1081 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa
     1141 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     1201 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     1261 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     1321 agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca
     1381 cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac
     1441 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     1501 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat
     1561 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     1621 tgtattcttt ttttgaacga taatcacaaa aaaaaatata atcgtaaatt tcaagtttaa
     1681 gttagccacc catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg
     1741 agtgggccct tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag
     1801 ctttccagac acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg
     1861 tcaaccgttc ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa
     1921 tctttcgcgg gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt
     1981 tgccgattgg gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg
     2041 tcccgcgctc ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg
     2101 catcgtgcag tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc
     2161 cgaagtacgc accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat
     2221 ccttcccggc cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca
     2281 gcacggcatc cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg
     2341 ttttccgggt accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat
     2401 tcgggcggtt gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca
     2461 tattcttcgt tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga
     2521 taatggccgg gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc
     2581 atttttccaa cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata
     2641 agggccgtct gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt
     2701 cgtagacgtc atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag
     2761 taacggtccc gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg
     2821 caacctgacg gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt
     2881 aggttaggtg ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaa
//
LOCUS       DADOOS010000214         2900 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.220, whole genome shotgun sequence.
ACCESSION   DADOOS010000214 DADOOS010000000
VERSION     DADOOS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.220"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            798..1064
                     /locus_tag="J8C37_002759"
     CDS             798..1064
                     /locus_tag="J8C37_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306155.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="J8C37_002760"
     CDS             1122..1634
                     /locus_tag="J8C37_002760"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5306156.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="J8C37_002761"
     CDS             1631..2668
                     /locus_tag="J8C37_002761"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ5306157.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2828..2900
                     /locus_tag="J8C37_002762"
                     /pseudo
     CDS             2828..2900
                     /locus_tag="J8C37_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1048 a          515 c          577 g          760 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctacaacg attaaaggca
//
LOCUS       DADOOS010000215         2885 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.221, whole genome shotgun sequence.
ACCESSION   DADOOS010000215 DADOOS010000000
VERSION     DADOOS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.221"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            106..357
                     /locus_tag="J8C37_002763"
                     /pseudo
     CDS             106..357
                     /locus_tag="J8C37_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="J8C37_002764"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="J8C37_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAZ5306158.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="J8C37_002765"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="J8C37_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="J8C37_002766"
     CDS             1786..1869
                     /locus_tag="J8C37_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAZ5306159.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="J8C37_002767"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="J8C37_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAZ5306160.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          976 a          462 c          575 g          872 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tctta
//
LOCUS       DADOOS010000216         2850 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.222, whole genome shotgun sequence.
ACCESSION   DADOOS010000216 DADOOS010000000
VERSION     DADOOS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.222"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            174..518
                     /locus_tag="J8C37_002768"
     CDS             174..518
                     /locus_tag="J8C37_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAZ5306161.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="J8C37_002769"
     CDS             519..938
                     /locus_tag="J8C37_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAZ5306162.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="J8C37_002770"
     CDS             complement(1030..1380)
                     /locus_tag="J8C37_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306163.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="J8C37_002771"
     CDS             complement(1346..2566)
                     /locus_tag="J8C37_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAZ5306164.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1062 a          506 c          544 g          738 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaaggcg
     2821 gattttataa ttaacttagc catttggaaa
//
LOCUS       DADOOS010000217         2810 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.223, whole genome shotgun sequence.
ACCESSION   DADOOS010000217 DADOOS010000000
VERSION     DADOOS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.223"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(23..1447)
                     /locus_tag="J8C37_002772"
     CDS             complement(23..1447)
                     /locus_tag="J8C37_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAZ5306165.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(1711..2688)
                     /gene="guaC"
                     /locus_tag="J8C37_002773"
     CDS             complement(1711..2688)
                     /gene="guaC"
                     /locus_tag="J8C37_002773"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAZ5306166.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT          793 a          612 c          445 g          960 t
ORIGIN      
        1 ttaatctatt tactttgtga aattattctg ttaaccactt ttctagaatt ttcatctttg
       61 aataaaacaa cggtatttgg ttttcatcca gcctactgta attgattctg attccattta
      121 tatcttgccc aaaaagtaga cttggtgcaa ttccaagacc catatctact actttttctg
      181 ctagttcggg gtctagtttt ttcccatccc aagtaatcca ggcatagtaa ccacctaaaa
      241 ttgaatgaag cttgaaaaac gattgtgaat ctccccaagc gcttatcagt cggctttttt
      301 ctttcaactt ttttcttaca tcttgcattt tcaaatcaat tgcttcatta aatacattag
      361 ccactgtgat ttgtgtaagg atattcatcg aaaactccat catttcttgc gcttgagcta
      421 gttgcgagat aaaagtctca ggtcctatca cccaaccgat tttcgttgtt tttcctaaaa
      481 ccctagataa cgaaccgacg tataacacat tatccggatc aatcgttcgt ataggaagga
      541 cttcttcttg gtttagaaat gaaagatccg taaatacatc gtcttcaata attggaagcc
      601 gatactgtcg acacaattct actagctttt ctcgtcgttt ttgactcatc acttttcctg
      661 tagggttttg aaaattcggg ttgataagca ctgctttcaa tttattttta tggatcgatt
      721 ctttcagttt ttctaagtcg atcccttctt catccatcgg tatcccaaat aaacgcgttc
      781 ctgaagcttt gaaaagaggt aatcggtaaa aaaaagaagg tgactctaca gctatcccct
      841 ctccagctga aagaatcgtt tgtaaaatca aaaagattgc ttgctgaccg ccaccagtca
      901 caagaagctg gttgggggaa aatgccaatt caaatttact ttccaaatac cgtttgattt
      961 tgttacgtaa cggagtatag cctaaaagat ccatttcttt ttcttccgct aagatttctt
     1021 caagagaata agaaggaaac tcaaaattcg gcaaaagctc atctggcatc tcgctagcgt
     1081 ataaatcaag aaacgatgtt ttttggatct gctttgcttc tcttattttt tgctcatacc
     1141 agtccgtctg ctcataacga ggagagatca tttgccgcca atcgattctt ggaacagcga
     1201 atttccccca ttcagtctga ttaacaaacc atccgctgcc acgtttgctc gttagtaccc
     1261 caagctccct aagctcttcc aatgcatgga caacggtgga acgattcaca ccaaaaagtt
     1321 gccctagttt tcgttctgat tcaagttgac ttccaggatt gatccttccg ttttcgattt
     1381 ctctcataat ctgttccata atttgacgat aaatcggtcc ttgttttttc tctacttttt
     1441 cccacataag cttctctttt ctattgtcta ttttttcttt tgattttacc gaatttacga
     1501 gaaaaaagaa aaactttttt tgcaaaacat aagggactgt gacaaaaatc ttatcacagt
     1561 cccttattct gaataatgat gttcaaaagc ttgtatctgc gataataatt ccagccaaac
     1621 aaaacatata aagaactatt tttgtttggc tgttcttatt acttaatgca ggacaacttt
     1681 ttcacaacat cctaatattt tatttatttt ttagattgtg tctccattaa aaatcgaatt
     1741 tttcacgatg acatagtcaa cttttcgaat ggcttccaga tctcttcctc cagcataaga
     1801 aatagatgat tgaaggtctt gttgcatttc cactagtgta tctttcaaag atcctttgtg
     1861 ttggatccag attttctttc cttcaacatt tcgtttttcg cctttttgga attctgaagc
     1921 agaaccaaag tattctttat acacgattcc attttcgact tttgtttcgc caggagattc
     1981 ctcgtgaccg gcaaacaatg aaccaatcat gaccattgtc gcaccaaaac ggacagattt
     2041 tgcaatatcg ccatgtgttc tgatcccacc gtcagcgatg atcggcttgc gagctgcttt
     2101 cgcacaccat ctaagagctg ccagttgcca gcctccagtc ccaaagcctg tcttgatttt
     2161 tgtaatacat acttttcctg gaccaatccc gacttttgtt gcatctgcgc ccgcattttc
     2221 caattcacgg acggcttccg gtgtaccaac attaccggca atcacaaaag tttcaggcaa
     2281 atatttttta atatgctgaa tcatatcaat aactgagtta gcatgtccat gcgcgatgtc
     2341 gatagtgata taatcaggat ttagcgattc ttctgccaac ttctctataa aggaatattc
     2401 ctctttcttg acgccaacac tgatcgaggt aatcaatcca cgctttttca tttttttgat
     2461 aaacggaatt cgagctgctt catcaaaccg atgcatgata tagaaataac cattttccgc
     2521 taagaattct gcgattgttt catcaataat cgtctgcatg ttggcaggca ctactggcat
     2581 tttgaatgta tgtttaccta atgtgaccgt tgtatcacat tcagaacggc tatttacgat
     2641 acatttatta ggaattaatt gaatatcttc gtaatcaaat actttcatta cattatcctc
     2701 ctgatgttca catccagcaa aacacgaaca ttattaattt aatcaaggct ttttttagcg
     2761 ccctatgtaa tttaactcaa ttaagcaaaa aaatcaagag caaaaagaga
//
LOCUS       DADOOS010000218         2615 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.224, whole genome shotgun sequence.
ACCESSION   DADOOS010000218 DADOOS010000000
VERSION     DADOOS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.224"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1017
                     /locus_tag="J8C37_002774"
     CDS             <1..1017
                     /locus_tag="J8C37_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAZ5306167.1"
                     /translation="EGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLN
                     KEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVT
                     DTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKG
                     TVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDG
                     SQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSK
                     GSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
     gene            1151..2494
                     /locus_tag="J8C37_002775"
     CDS             1151..2494
                     /locus_tag="J8C37_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAZ5306168.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1047 a          329 c          488 g          751 t
ORIGIN      
        1 gaaggtgtca ttgaagtaac gtatccagac ggtacgaaag atacggtgaa agttccagta
       61 gaagtgacag acaatcgttc taatgcagat aaatacaatc ctgaaggaca aaaagtgact
      121 acagacttaa ataaagagcc agatgcatct gagggaatta agaacaaaga agatttacca
      181 aaaggaacta cttatgcttg gaaagagaaa gtagatgtta gcacagctgg aaataaaaaa
      241 ggtactattg tagtgacata tccagatgga tcatctgatg aagttgaagt agacgttaca
      301 gtgacagata ctcgttccga tgcagataaa tataatcctg aaggacaaaa agtgactaca
      361 gacttaaata aagagccaga tgcatctgag ggaattaaga acaaagaaga tctaccaaaa
      421 ggaactactt atacttggaa agaaaaagta gatgttagta cagctggaaa caaaaaaggt
      481 actgtagtag taacatatcc agatggatct aaagaagaag tagaagttac tatttctgta
      541 gtagataaaa aagcaccgaa taaacctcaa gttgatccta ttacagaggg tgaccaaatt
      601 gttactggta aaactgaacc aaatgcagag gtgacagtta cattaccaga cggaagtcaa
      661 caccatggga cagctgataa aaatggtaat tttacagtta aagttcctaa attagaggca
      721 gggacaaaag tgatagtaac tgcaactgat gaatctggta atactagtga acctactaat
      781 gtagttgttt cttccaacaa aaaagacagt ggaaaaaacg gtagtaaagg tagtaagact
      841 gacaatcaag gtagcaacag caatcaagac aaaaatagag gcaaaagcca gtcctcaaaa
      901 gtttttccta agacgggaga gagtgacagt aatatcttta ctattagcgg tggtttaata
      961 ctgttaggaa cgttaggatt attagggtat aaaaatcgaa aaaaagagaa tgaatagaaa
     1021 atcaatttct taagtcttct ctgagaagag gaagaatctt tttcttcctc tttttgcttt
     1081 tttatgatgg gaaataagtg ttagttaaag atgaatatac tttttataga aaggaaaaga
     1141 atgtaattaa gtggaaaaaa tagtaattat tggttcaaac cattcagggc tagccgcagc
     1201 tcgatttcta aacttaagcg attcaaatta tgaagttatt ctaatcgata aaaaatcaga
     1261 aattagttac ttggaatgtg ggacatcgtt atttataaaa ggagaagtaa gtaatttaga
     1321 agatcttttt tatcaaaata gaatgacact aaattctgag ataaaaaagt tatttctaga
     1381 gacagaagtt gacaaaattg atattcaaaa taaaaagatt agttgtgtaa aaaaagatgg
     1441 tacaagattc tctcaagaat atgacaaagt tatattagct acaggttcaa agcaaattat
     1501 gctagatata ccggggaata atttagctaa tattttttcg ataaagaatt ttccaaatgc
     1561 aaaagagatt tttaaagctt tggatgatag tactattaaa aaagtaggaa ttattggagc
     1621 aggatatatt ggagttgaaa ttgctgaagc cataagaaaa agagggaaag aagtatatct
     1681 tttcgatgtt gcagatagag tgttgtcgac ttattacgac cgtagttttt cagataaagt
     1741 tgaagaaata ctatcaaaaa atggaataca tctttgtcta tcagaaagtc ctaaaaaata
     1801 tttaggaaga aaaaagattg aaaaaatagt cactacaagt ggtacccaat actctatgga
     1861 tatggtcgta caagctatag gatttctatc taatagtcca attggggaaa aggaactctt
     1921 gagagatgtt agtggagcat atctaactaa tgaataccag caaacaaata taaaagatat
     1981 atatgctatt ggggattgtg ctacaacttt ttttacgtcc attaatagaa gtagtgttga
     2041 tttttctatt tctaatgctc taagaacagc ttatattgca actcaacata ttaacaatca
     2101 agattttaca ccttctggtt cccaatttac aaatggcttt tctatattta attacaattt
     2161 ttacgcagca ggtttgaatt taaaaacagc acgattatta agtgagtcta tagattatat
     2221 agaaatagaa gatttcatta gacctgcttt tttgagaaaa aatagtcgtg taaaattaag
     2281 aattagcttt gaaaaaaata caaagagaat agtaggtgta caactttgta gtcaagaaga
     2341 tttatctgga ctattatcaa tgttttctct tgctatagaa gaacaaatta cattagacaa
     2401 attgaaaatg ttagattatt tatttcatcc tagttttagc caaccctata attttatttt
     2461 aaaggcagta acaggattaa acattgaaac ataatcgtta ctaaatgaaa tcaagaaata
     2521 tactgtatta ctcaattcat tgaaaatatt tatcataatt tttaatagaa aacactatta
     2581 aagagaaaaa agagaggtaa atcaaatgaa aaaat
//
LOCUS       DADOOS010000219         2593 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.225, whole genome shotgun sequence.
ACCESSION   DADOOS010000219 DADOOS010000000
VERSION     DADOOS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.225"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1042)
                     /locus_tag="J8C37_002776"
     CDS             complement(<1..1042)
                     /locus_tag="J8C37_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAZ5306169.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFL"
     gene            1231..1992
                     /locus_tag="J8C37_002777"
     CDS             1231..1992
                     /locus_tag="J8C37_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ5306170.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2107..2547)
                     /gene="fabZ"
                     /locus_tag="J8C37_002778"
     CDS             complement(2107..2547)
                     /gene="fabZ"
                     /locus_tag="J8C37_002778"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAZ5306171.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          806 a          528 c          451 g          808 t
ORIGIN      
        1 ggaggaataa ttcgtcatcg gagattcttc ctacaacatt gacacgtccg tcgtttttca
       61 tatccttcaa ggcgatttgt gtttctcctt tgtattgacc gtacccttca ttgtcaatca
      121 gtacatcccc atgatgcata tctcgcgtat tatgaggcgg gaaatcttta tctttgtagt
      181 atacacgagt catcgttgaa cgaaggatat attctgagcg atcccctcga taactatgaa
      241 ggttatcaaa caagcagatt cgttcgtttt ctgtaatacc ttcttccgta tctactttca
      301 atgttggata atccgcgtta aatgcttctg ccatttcttt caattcttct tcacttgcgt
      361 acgcatttcc aatagaaata tcatcaatca atcctgtaag gattaaatgc ttcacttgtg
      421 tcgcaatttc aagatctcgg tggtcttcca gcgtgcacaa gccatcttgc gtaggccaag
      481 ggccaaactc agcagattgc gaattgacga atgccatcgt attcaaatta tattttctga
      541 atttttctga acaaaaaaca aagtgttcgt atcctaaacc agagtatcga tgaggataaa
      601 aattatgaga acctaataaa ttatccgtgt ttggcgaata actcatgatg ttgtcaacgt
      661 aattcgtgcc cgaactcata ttgatctcga ttttgatacc aaaaggattg cgcgtcattt
      721 ttgcttcttc tgctcctgtg aatccgatat ctaagcggac cccgtaggca cccatcttat
      781 ggaaaaaaga taagtcatcg taagaaatct ccaactgttc aaataacgct ggattgatat
      841 caaccattac ttccatgcct aggctattcg cgtaatcaac tacttcttta aattctgaaa
      901 gaactttttc tttatcatca ttgatctgca gcaaactggt aaaaacccgt ttatacccat
      961 acttatgagc taaatccaag tacgctttgt ccttttcaaa tgttgaccgt tctggataaa
     1021 tcgagattcc taattttccc atattaccat ttcctcctgc atcttttcgt ttttgtatcc
     1081 acttacatta tagcaaacac aaagtaaatg gtctacacct tttctttttc ttttaaaata
     1141 aatgataaat tccaataatt taagtaggta ttttccctct attttgttat cattaaagat
     1201 aacaaaatag taaattggaa aggaacagtt atgaagaaaa ataaagttat tttaagtatt
     1261 agtttagcag cagcattgct gtttgctgct ggttgttctg caaacaacac gacttcttct
     1321 agttcgtcta cagaaagttc aaaaagtaca tctactgcgg aaagcacgac tgccagtagc
     1381 gtcgatttga acgcattgga attaccacaa ttgacgaaag atgtcgccga aaatgaagat
     1441 ctggtcgaaa tggtgacgaa taaaggcaca atcaaaatca aattatttcc cgaacacgca
     1501 ccaaaagcgg ttgaaaactt tatgacacat gcaaaagacg gttactacaa cggactgact
     1561 ttccatcgcg tcatcaaaga tttcatgatc caaggtggcg atcctaaagg cgatggcact
     1621 ggaggggaaa gcatttgggg agaaggattt gaaacagaga tcaacaacca cctttataat
     1681 ataagaggtg cgttatctat ggctcgctca caagatccta acagtaacgg cagccagttc
     1741 tttatcgttc aaaatacaga tgatatgcat gatggtcttt taaaagacga ctatcctcaa
     1801 gcaatcatcg acgcttataa aaacggcggt tatccttcac tagatggcca ttatactgtc
     1861 ttcggccaag tgattgaagg aatggatgtc gtggatgcta ttgcaaatat cgaaacagat
     1921 agttcagaca aaccaaaaga agaagtaaaa atcgataaaa tcaatatact gcaagaagca
     1981 aaacaaaaat aaaaaaataa cgtctgaatc ctgcatagat gacaacgtat gctaggattc
     2041 aggcgtcttc tttttacata aaaagcaagt aagcttcctt ttgttttgct atatcaggtt
     2101 ttatagtcaa gcattttggt tgacgatgaa cgtgaactcg cactcacaga cttttttatc
     2161 atctacatat gctgcagata acgctgtacc gactgatccc ttcattttgg tgatttcaaa
     2221 atgacatttc agcacatctc ctggaacaac tttttgacgg aatttggcct tattgatccc
     2281 accgatataa gctctttttc ctagaaactc ttctgatttt aggataagta tcgaacctgc
     2341 ttgtgccagt gtttctagaa tcaacgcacc cggcatcact ggattgtcag gaaagtgacc
     2401 ttggaaaaaa tcttcattga ttgttacatt ttttgtcgca acgatctttt ttcctggttc
     2461 taaagtatct acataatcaa tataacaaat aggataacga ttaggaatca attccattac
     2521 ttcagtagca gataatacgt aagacataat taaactcctt ctttcaggca attgccattt
     2581 tcattacttt gac
//
LOCUS       DADOOS010000220         2567 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.226, whole genome shotgun sequence.
ACCESSION   DADOOS010000220 DADOOS010000000
VERSION     DADOOS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.226"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..1342)
                     /locus_tag="J8C37_002779"
     CDS             complement(<1..1342)
                     /locus_tag="J8C37_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAZ5306172.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERD"
     gene            complement(1361..1765)
                     /locus_tag="J8C37_002780"
     CDS             complement(1361..1765)
                     /locus_tag="J8C37_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAZ5306173.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1997..2254)
                     /locus_tag="J8C37_002781"
     CDS             complement(1997..2254)
                     /locus_tag="J8C37_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAZ5306174.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          780 a          487 c          350 g          950 t
ORIGIN      
        1 aatctctctc tatttcctta acatactgtc taaatcgaat ctcttgatag gtgggatatg
       61 ttaaagctga attattttgg atatcaacat tcttttgtct aaaatatcta tcatatttct
      121 caaaaagctg ctgcttatca gtgtctttct tccacgtttg ataaagatac tctataagtt
      181 cgttgatatt aaaaacttcg tctacttttt ccaaatcatc catttgaata tattttaatt
      241 cattctgtct tttcataacc ttcatgccgt tgaaattcac ccattgtggt aaatgataca
      301 tctgaatata aagttcttcc aaatcatcta agaaataaaa ggcattggca caggtcaatt
      361 gaatgatatt tttcaaattg ccaacatttt cactaaagcg atagtttaat aaaaaatcta
      421 ataattgttg agatatcagt attttctttg ctagtctttc ttcttctgat ttaaaaaata
      481 tcttgattaa ctgttttctt tcataaagag aacgactgtc taatgttgga agtgttagct
      541 ttacaggtat tcttctcaac aaagttgata atagtttatc ctcaggtggt tctgatgttg
      601 caaatataat ccagcaactt gatgtgtacc agttttcatt atcacccatc cgatgaaatt
      661 tcctcgtatc cataaactga aaaagtttct cctgacattc attgctcaat gcatgaattt
      721 catctaaaaa taagactcca ttattagcta attctgctaa accctttctt ccatcttcgg
      781 ctcctgtata tgcacctttt acatgcccaa aaaaattgga caagaaaagt tctggattat
      841 tggcatattc actacaatta actgaaatga aagatgcctt ctcttctata taattattgg
      901 ccaacaaatc cttatacaca taatttgcta aatagctttt tccagttcca gtctcgccat
      961 gaagtaaaat gggtaagcca tttggcggat agtagattgc tgctttaatt tgagcaatta
     1021 catttcgcag acttccttga cttccgataa ccctttctat tccaacccta aaagagctat
     1081 cataccattt ttccgcttcc tctgaagagc taaaaatttg ttgatttgga actttctccg
     1141 gtaaagaaaa gaagattact ggacgagtat tgatttttcc aaattctcca tcttttacga
     1201 attcattcaa atattgagag acaatattac gactaacatt taatttttca gatattttct
     1261 gtgctgtatt gttcccccat ttatttttaa tctcagagtt ctctatgaca taattgacaa
     1321 tgcgttcttt taattcttcc atcacagcac ctcctcgaca ctattcgaat tttgccgaca
     1381 gtatttttcc ataaatatga cttgctaaaa aggctttgtt tagatcattc aattggaacg
     1441 ttgcttgttt tgcttgatcc gctaagacgt tgaaaagata ctctggcgaa atcgcatacc
     1501 ccaaaatatc aacgattgtt gcaatcagat cttcttttga ttcttcatct gaaagtaatt
     1561 cacgatacaa catcgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta
     1621 aaagaaccac ttgttccaac aaacggtcgg ataaatattt atagaaaatc agccccaata
     1681 aataattttt atattctgac gcatccattt tactacggag attatctgcc gcactaaata
     1741 acttctgatt taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta
     1801 tataccctct tattttagca tactcagttt tagaaaaagc aaaaaaataa agaaacttat
     1861 tcttgataac aagctacgtg acgtatatcc ctaacacaaa aatagccata tcctttggtt
     1921 tggatacggc tatttgatat tactcattca attatcaaat cacagtaggt gtgaatctca
     1981 tcgggatcgg caatatctac ttctgattag gatcaaaggt gattttctca ataaaggtgt
     2041 gatacaaagc tttaacttgc ttcttctcac tctggataag aaagcaatcc aaactagtca
     2101 atatctgttg gacatctttg acttgaatcg gttccccttg ttgttgaaag atccctaaga
     2161 tttcattctc tcgaatttgt aactgtcttc gtttttcagt caattcattt aaacgatctt
     2221 ttaacatcgg aaacaattcg ggactatctt ccaaagcagc ttcctgtttt tgctctaata
     2281 gttagatttt gcgtaccatt ttcttctttt attttccttc gattgctgat tttaatatag
     2341 tctaaaggaa atttatattt attgtattct ttataacttc aagagaactt cgtttgagta
     2401 ttactgtatt tttttaattc cataagaaaa ttagttatta cctgccacct aatcagcaaa
     2461 tctgaacctg agtcaatata attaatatta ttttccatct ccaacattgt aagtactgtt
     2521 ggtgttaata tagggacttt gtacaattgt atttttttga ttttatc
//
LOCUS       DADOOS010000221         2548 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.227, whole genome shotgun sequence.
ACCESSION   DADOOS010000221 DADOOS010000000
VERSION     DADOOS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.227"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..386
                     /locus_tag="J8C37_002782"
     CDS             <1..386
                     /locus_tag="J8C37_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306175.1"
                     /translation="EEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFA
                     NTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYN
                     YIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            711..1328
                     /locus_tag="J8C37_002783"
     CDS             711..1328
                     /locus_tag="J8C37_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306176.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1369..1668
                     /locus_tag="J8C37_002784"
     CDS             1369..1668
                     /locus_tag="J8C37_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306177.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1702..1869
                     /locus_tag="J8C37_002785"
     CDS             1702..1869
                     /locus_tag="J8C37_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAZ5306178.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1914..2519)
                     /locus_tag="J8C37_002786"
     CDS             complement(1914..2519)
                     /locus_tag="J8C37_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306179.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          886 a          390 c          474 g          798 t
ORIGIN      
        1 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
       61 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
      121 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
      181 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
      241 caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag
      301 aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag
      361 aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc
      421 aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact
      481 agtttattta atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat
      541 ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga
      601 ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt
      661 aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat
      721 ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga
      781 aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta
      841 aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac
      901 aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa
      961 ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt
     1021 acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct
     1081 atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg
     1141 cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg
     1201 cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg
     1261 gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact
     1321 tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg
     1381 aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt
     1441 ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg
     1501 gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa
     1561 accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt
     1621 tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa
     1681 tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata
     1741 actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa
     1801 agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat
     1861 ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa
     1921 tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc
     1981 tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt
     2041 tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt
     2101 tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca
     2161 atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca
     2221 gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata
     2281 aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg
     2341 atgacgcaat caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta
     2401 tctttaccac tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc
     2461 aattgtcgat ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata
     2521 aaaaactcct ttttaaaagt gatataat
//
LOCUS       DADOOS010000222         2515 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.228, whole genome shotgun sequence.
ACCESSION   DADOOS010000222 DADOOS010000000
VERSION     DADOOS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.228"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..179)
                     /locus_tag="J8C37_002787"
     CDS             complement(<1..179)
                     /locus_tag="J8C37_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAZ5306180.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="J8C37_002788"
     CDS             complement(192..1295)
                     /locus_tag="J8C37_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAZ5306181.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="J8C37_002789"
     CDS             complement(1385..2320)
                     /locus_tag="J8C37_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAZ5306182.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT          632 a          520 c          449 g          914 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaa
//
LOCUS       DADOOS010000223         2492 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.229, whole genome shotgun sequence.
ACCESSION   DADOOS010000223 DADOOS010000000
VERSION     DADOOS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.229"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            359..1468
                     /gene="pdhA"
                     /locus_tag="J8C37_002790"
     CDS             359..1468
                     /gene="pdhA"
                     /locus_tag="J8C37_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAZ5306183.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1471..2448
                     /locus_tag="J8C37_002791"
     CDS             1471..2448
                     /locus_tag="J8C37_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAZ5306184.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          826 a          467 c          525 g          674 t
ORIGIN      
        1 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
       61 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
      121 aaaaatgcag atgaaaaata aaacgctttc cttattgtga ttttattcac taatgctgtt
      181 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
      241 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
      301 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
      361 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
      421 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
      481 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
      541 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
      601 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
      661 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
      721 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
      781 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
      841 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
      901 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
      961 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
     1021 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
     1081 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
     1141 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
     1201 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
     1261 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
     1321 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
     1381 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
     1441 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
     1501 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
     1561 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
     1621 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
     1681 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
     1741 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
     1801 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
     1861 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
     1921 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
     1981 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
     2041 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
     2101 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
     2161 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
     2221 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
     2281 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
     2341 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
     2401 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
     2461 cgaaacaaag tcatggctta tcaatttaaa tt
//
LOCUS       DADOOS010000224         2407 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.230, whole genome shotgun sequence.
ACCESSION   DADOOS010000224 DADOOS010000000
VERSION     DADOOS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.230"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            5..1150
                     /locus_tag="J8C37_002792"
     CDS             5..1150
                     /locus_tag="J8C37_002792"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAZ5306185.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="J8C37_002793"
     CDS             1321..1779
                     /locus_tag="J8C37_002793"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAZ5306186.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="J8C37_002794"
     CDS             1938..>2407
                     /locus_tag="J8C37_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAZ5306187.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DADOOS010000225         2375 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.231, whole genome shotgun sequence.
ACCESSION   DADOOS010000225 DADOOS010000000
VERSION     DADOOS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.231"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            134..484
                     /locus_tag="J8C37_002795"
     CDS             134..484
                     /locus_tag="J8C37_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5306188.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            898..1716
                     /locus_tag="J8C37_002796"
     CDS             898..1716
                     /locus_tag="J8C37_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAZ5306189.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1789..2148)
                     /locus_tag="J8C37_002797"
     CDS             complement(1789..2148)
                     /locus_tag="J8C37_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAZ5306190.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          786 a          343 c          444 g          802 t
ORIGIN      
        1 tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga tgggtataag
       61 tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag tgtaacataa
      121 atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag caatcattcc
      181 tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa ttgtcggttt
      241 tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt ttattggtaa
      301 gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa aaagaattga
      361 tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat ttgaaattga
      421 atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt tagtaaaaaa
      481 ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat ttttattttg
      541 atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca ttcagtaata
      601 aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac cagttataga
      661 aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag gctgacctgt
      721 gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt atgtatctag
      781 atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa taggctgctt
      841 taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag tgtttttatg
      901 aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac atttgctcgt
      961 actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat gaaagctgtt
     1021 gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc gttatttcca
     1081 aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa agataaaaca
     1141 gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct tgatttatgt
     1201 aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta ctgtgaaaga
     1261 ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag attagaatgg
     1321 gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac tatgcctgtt
     1381 gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct attagtacct
     1441 accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa aactttcggc
     1501 ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc ttttggagat
     1561 gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat ggataatgca
     1621 acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga agacggtgtt
     1681 ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc atcaaaaaac
     1741 ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt acgaagcgtc
     1801 tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat tctttaaacg
     1861 aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa aggtagtatc
     1921 gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat atgctggaat
     1981 ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc cctctgctga
     2041 agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta aacgaggtac
     2101 ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt tcacgaccac
     2161 catcgacttc cacagttgaa attgacattt tatttaacac acctattccc ccttaactat
     2221 aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc aaatcgccaa
     2281 tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg taaaaattaa
     2341 accgaagatc tgataaattt tctctatttt ctaca
//
LOCUS       DADOOS010000226         2295 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.232, whole genome shotgun sequence.
ACCESSION   DADOOS010000226 DADOOS010000000
VERSION     DADOOS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.232"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            102..1964
                     /locus_tag="J8C37_002798"
     CDS             102..1964
                     /locus_tag="J8C37_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5306191.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1964..>2295
                     /locus_tag="J8C37_002799"
     CDS             1964..>2295
                     /locus_tag="J8C37_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAZ5306192.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          825 a          341 c          452 g          677 t
ORIGIN      
        1 attagttaaa aagctataag ttgcacttag ttaagtgcaa acatttcata ttgtcgttag
       61 tttagaaaac gcttatatta gcaatgtaag gagagatgat aatgcagcag ctaacaaaaa
      121 gagaaaggca gcttttgatt tacttggtgg aaatgtctga accggtaaca atcaaaaagt
      181 tggctgaaaa agaaaatgtt agtgttcgga cgataaaata cgatctagat gatataagag
      241 agtggctgaa tgagcggaag caagtcctat atagcaaacg tagtcaggga atttggatga
      301 atattagtag ctctgatcga atcaaattaa aaagcgaatt gatggatgtt aatcgttttg
      361 aaatatttgc agatcaaagt ctccgaatca aacgtttact aattatgctt ttagtaacaa
      421 aagaagctat tactggaatg aaaatggcca acgcattaga tgtcagcaaa gatacgatca
      481 tgaatgattt agatgtgctg gaaaataatt ttcttaagga aggattgacc ttgaatcgtc
      541 aggcgcgaaa gggtttttgg atcataggag aagaacgtct agttcgatta acgattgaag
      601 agattcttca aaaagagttc acggattatg acatttataa attaatgtca ttgttattga
      661 atggcggtga gactgaatac tttgaaatgt attctgctac tgctacacct atccaagagg
      721 tatttaatca agtaatcatt cgaatgcgac acttactgga atttgaaaat ctagagaaac
      781 tgaattacgc ggaattacta aatattctga ttcgagtgac aattgctacc gttcgcttac
      841 gcaaagaggc tacgattggt cgttatcaat tggttgctga acaagaatta gacaaaaacg
      901 atctttctta tcgcgtcatg cagcaggtct ttgattatta tcagttgcca ctgttcgagg
      961 atgaatatca ttatatctat agtgatacat tcgacacgat tcaccagcaa gacgtcatga
     1021 aactagttga gaatgtgatt tataaagtaa gtaaagcaat caaccattct ttttatcaag
     1081 atccacaatt gttgacgaat ctgtatgctc atttatccat gcgattatcg agaaagaaaa
     1141 aatttgtcaa tgaatataat ccttttaaag aagatatcaa gcagaaatac ccggaattat
     1201 ttgatgcgat caagcaagca gttcgacaag aaataagtga acaaaatatt ctgatcaatg
     1261 attctttcgt ggcatatatt gccttacact ttttggtttc ctacgaaaga gaagaagatc
     1321 tacgaagcgt tagagccgtc tacatttgtt caacagggtt gggtgtgact agtctgatca
     1381 agcaaaagat caatgaagaa ttaaataata tcactatcgt ggcatttgct tcagtattga
     1441 atgcgcaaga aatgattgct accaaagatc ctgatttggt gattagtatt tttccaatta
     1501 atggcatcga tcgtcctttc atcaaagtcc atccattacc cactgaacag gacttgaaaa
     1561 aaattcaaca aataataaaa aaaatacttg cttcttccaa tcagcaggaa aggcataact
     1621 tgaatcttag agaaacgtcc atcaataaag atactttaga agactttagc aaggaattga
     1681 ttttgaaagc ttatacgatt tatgaacgtt tacttgtcat tttggaagca gaattgatcc
     1741 aagaatatcg agaagctttt ttgcttcatg tattcttaat ggttcatcgt atcacatttg
     1801 atcaacaata tatggcagaa ggtcgtcaag tagaccagat tattttagac aaaggaacac
     1861 tggtaggaaa aatcgaagca gtttttgcag aaaataattt aggaatcaat cagtcagaaa
     1921 tcagtgcatt attctcatat ataaaagaag gagcgtcaag ttagtgaaaa tgtcagacga
     1981 ggctttaaaa attatcgagg aaagtcaaaa tcgggaaaag ttaaaacaag tgattgatct
     2041 aattgaagag cgattagtag aacatgctat tgtaccaacc gagttgcagt ggaccattct
     2101 aattaatcac ttgaatgaga tgctgaaacg ttctcaaaga aaggaaacga ttcccgtagt
     2161 cgatcgggat ttgttcaaag aagtatccaa agaagccata actatttctg atgaaatagt
     2221 tagaagtatc ggtaatttaa ccgatgatga aatatatgtt ttgtcgattc attttgaaac
     2281 agcaaaaaat aatta
//
LOCUS       DADOOS010000227         2266 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.235, whole genome shotgun sequence.
ACCESSION   DADOOS010000227 DADOOS010000000
VERSION     DADOOS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.235"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(153..2150)
                     /locus_tag="J8C37_002800"
     CDS             complement(153..2150)
                     /locus_tag="J8C37_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAZ5306193.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          577 a          508 c          411 g          770 t
ORIGIN      
        1 acaatttttg tcacaacctt ttttgccgag taaacggtgt tcaaaagcca gtttctcggt
       61 atgaagtcaa atttaagcaa aaatgtgaga agctattttc acaaaatttt tcttcatact
      121 cgaaactaag cggctttttc acgacctctt ttttattttg ccatgaacat atcataatac
      181 gcgtccatta tttctttagc aatcgtcaag tttatgtggt cttgttcgtc taataagttc
      241 ggcaagacaa cactgatggc aatttccgga ttgtctgttg gtccgtatgc cacgatatta
      301 ctattgactg tcgcaatatc tggatgacct tccgcaaccg tttctgcagt ccctgttttt
      361 gcggaaaggt ccattttcgc actggctaat ggtttggctg ttgtgtaagg atcggtacca
      421 tggacaactt ggtggaatcc ttcacggagg atcgccatat tttctgcaga aatattgacc
      481 gtattttcta ctgttgtgcc aatttctttc ttcacttcac ccaagtttcc attttcgtca
      541 ttgccataga tcgctttgac taggtgcggc tgtactcgtt tcccgccatt agcgactgtt
      601 gaagcatatt gagctaattg gattggtgta tatgtgtcaa actgtccaaa tgccaaatcg
      661 gtaaagttac ctggagcata ccattctttg ccattatccg cattgaactt cttcatataa
      721 tcaacagaac gtgagatccc aggagattca ttcggcaaat cgatcccagt cgttgtacca
      781 agaccaaact ctttgaaaga gtctctcaat tgctgataag ctttagcttc ttcatctaaa
      841 ctaggtagtc ccattccagg tgtatagtct agtcctaaca tctttaacgc cactttgatc
      901 atatacgtat tagaagacaa ttccgatgct ttgactgcat caagagcaac ttgtccgctt
      961 cgattaaaga cagagctttt ttctgaagca ccttgcagac gaatcggctc atcaatcaaa
     1021 acttgatttc cgctgatcgc gttacttgcc cagcctgctg tcaaagtacc agccttgact
     1081 acagaccctg gatcaaaggc gctagtaatc gtgcctaaag cattctcggt gatttctttg
     1141 gaaccttgtt catgactgaa accagtcatt gctagcactt ctcctgtctg aggattcata
     1201 gccactgcat aagcaccagg cgaatactgt gctttgccgt tgttgatcaa tgtttgataa
     1261 ttccttttca aaatttcttc tacttttgat tggaattctg cattcataga taacattaaa
     1321 ttagaacctt tttccccaga gaagatttct ttttgattcg aaacatttcc ttcattatca
     1381 agggaaactt catattgggt tttggttcct tgcaggacat cttcatattg tttttccaaa
     1441 taactttgtc ctacacggtc attccgagaa taaccttttg ctaggtattt ctctgcttca
     1501 tctgctggaa gtccttgctt ttcagtcgtc acactgccaa gaatactttt cagtgaactt
     1561 gctgcattat attctcgtgt ccagtctgtg ccagtcgaaa ctcctggaag ttcagaagcg
     1621 cgttctgcca caacagctaa ctcttgatca gtaacaccag agtttttgat atagacagtg
     1681 tttaattcat atgcactatt catccgttta aagatggtag caatttttaa ttgctcctcg
     1741 tttaaattaa tctcatcagc tgtgacttta ttgaccatcg cactatattg ttcgctgttc
     1801 cctaattgct tttcttttgc cgaaagtctt tttgtggctt ctttcaaatg tttgggatca
     1861 gccagccaaa aatctttttt atctctatct gtcagatttt catctaccgg aacatcgatc
     1921 agcgagttca gcttcgttgc gatttcaagc aaatctttag ccgtaacttg tgttcctctc
     1981 gtatatgtga tggcctgatt cgctttattc gtaactaaag cttttcctgt ggagtcgtag
     2041 atcattccgc gaggagtact ttcttggacc gtaatcgtag aagtagactt cactcgcgcc
     2101 tccatctctt cgccgttcac gatctgcaaa taacccaatc tagcaatcaa cgcaacaaat
     2161 aacgtaaata taatgaaaaa aagaaaattt aagcgaaaag gaacatggga ccggcgaaac
     2221 gttttgttct taggttcttg tgcacctttt cttatttttt ctaacc
//
LOCUS       DADOOS010000228         2232 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.236, whole genome shotgun sequence.
ACCESSION   DADOOS010000228 DADOOS010000000
VERSION     DADOOS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.236"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..543
                     /locus_tag="J8C37_002801"
     CDS             <1..543
                     /locus_tag="J8C37_002801"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ5306194.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="J8C37_002802"
     CDS             complement(899..1471)
                     /locus_tag="J8C37_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306195.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="J8C37_002803"
     CDS             complement(1487..2092)
                     /locus_tag="J8C37_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAZ5306196.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DADOOS010000229         2218 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.237, whole genome shotgun sequence.
ACCESSION   DADOOS010000229 DADOOS010000000
VERSION     DADOOS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.237"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            121..2205
                     /gene="cadA"
                     /locus_tag="J8C37_002804"
     CDS             121..2205
                     /gene="cadA"
                     /locus_tag="J8C37_002804"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAZ5306197.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          724 a          352 c          508 g          634 t
ORIGIN      
        1 aaattttccg atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac
       61 cattcattta taattagtta tgaagagatg ttcatatgtt gaggcgagga ggtcattaaa
      121 atgaaaagtt acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa
      181 ggagttcaga agatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta
      241 acgattgacg cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg
      301 aaggaattag aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat
      361 ggaaatgaaa aagagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct
      421 ttattgctat tatttgattt taatgaacca attcgcttga tagggtattt attgatttat
      481 ctgctgatag gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc
      541 tttgatgaga attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat
      601 ccagaagccg tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca
      661 gtcagtcatt caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac
      721 gtgcagacga cggaaggatt gatgaccgtg aaccctgaag acgtgcagat cggtcagttt
      781 gtacttgtga agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca
      841 ttagtcgata cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa
      901 acagtattaa gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct
      961 tatgaaaatt ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa
     1021 gcaccagctg aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga
     1081 ttggccgttt tattagctgt tttacctcca cttgttgttt ctggagcggc gttttctgaa
     1141 tggatctatc gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc
     1201 gtgccactta gtttctttgg cggtatcgga ggcgcaagta aaatcggtgt attagtaaaa
     1261 ggttcaaatt acttggagtt gcttgcgcaa accgagacag tggtattcga taaaaccggc
     1321 acattaacga aaggtgactt ttcgatccag acgatcgata caaaaatcga tccaaagaaa
     1381 ttcatgcaat atattgcgag tgcagaacag ttttctacac atccgattgc tcaatctgtt
     1441 ttagaaggct acgcaggtcc gcttttacca acaactaata tccaggaatt tgcaggtgaa
     1501 gggatcttag ctgaagttga tggcaaacaa gtattagcag gtaatcacaa attgatggaa
     1561 agatttgaaa tctcttttcc tttaagtcaa gaaatcggta cattgcttta tctagcgatc
     1621 gatcaaagtt acagtggtta tcttgttatt gcagatacct tgaaagaaga tgccgtagac
     1681 gcattggttc aattaaaaca ggctggtgtg aagaacacag ttatgttgac gggcgactcg
     1741 aaaaaaattg ctgatcatat cgggaagcaa gtaggcgtgg acaagatata tagcgaattg
     1801 cttcctgaag ataaagtgca gcggttagag gaaatcttac aaagaaacaa taaaaagacc
     1861 gcatttgtcg gagatggaat caacgatgca ccggtgctag caagagcaga tgtaggaatc
     1921 gcaatgggtg gattgggaag cgatgctgca attgaagcag cagatgtagt catcatgaat
     1981 gatcaaccat cgaagattgc agaagcaatc catttagcaa agaaaacatt gaaaatcgtc
     2041 aaacaaaaca ttgtctttgc tattgggata aaaattttgg tacttgcctt aggtgctttt
     2101 ggttttgcat caatgggaga tgcagtattt gcagatgttg gggtgacagt attagctgtt
     2161 ttgaatgcca tgcgaagctt gcatgtaaaa aaagaggacg aatgaacgtc ctcttttt
//
LOCUS       DADOOS010000230         2217 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.238, whole genome shotgun sequence.
ACCESSION   DADOOS010000230 DADOOS010000000
VERSION     DADOOS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.238"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(440..1924)
                     /gene="guaB"
                     /locus_tag="J8C37_002805"
     CDS             complement(440..1924)
                     /gene="guaB"
                     /locus_tag="J8C37_002805"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAZ5306198.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          592 a          482 c          404 g          739 t
ORIGIN      
        1 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
       61 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
      121 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
      181 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
      241 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
      301 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
      361 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
      421 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
      481 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
      541 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
      601 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
      661 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
      721 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
      781 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
      841 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
      901 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
      961 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     1021 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     1081 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     1141 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     1201 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     1261 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     1321 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     1381 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     1441 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     1501 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     1561 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     1621 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     1681 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     1741 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     1801 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     1861 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     1921 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     1981 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     2041 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     2101 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     2161 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagta
//
LOCUS       DADOOS010000231         2168 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.239, whole genome shotgun sequence.
ACCESSION   DADOOS010000231 DADOOS010000000
VERSION     DADOOS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.239"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            71..2005
                     /locus_tag="J8C37_002806"
     CDS             71..2005
                     /locus_tag="J8C37_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAZ5306199.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT          666 a          395 c          506 g          601 t
ORIGIN      
        1 taataagtat caaactaaaa gaattatcat atattcatta tatagatagt ctataaggag
       61 cggatgatca atggcaaagc aaaatattgt cgttgtagga gcaggatatg ccggtgtttc
      121 agctacaaaa tacttggcaa agaaactgaa aaaagaagat gtagcaatta ctctgatcga
      181 tcgtcattcg taccatacta tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga
      241 accagaagca atccaatacg atctgcagca tttgtttgct cgtcaaaaga atgtacaact
      301 tgtcacagac actgtaatcg gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg
      361 ttcctatcct tttgatcagc tgatccttgg aatgggcgga gagccgaatg actttggtac
      421 acctggtgtc aaagcacacg gattcaccct ttggtcattt gaagattctg taaggattcg
      481 ggaacatatt gagaagacag tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa
      541 agccatgctg actttcgttg tctgtggttc tggatttacc ggtattgaaa tgattgggga
      601 actgatcgac tggaaaaatc gcctagccaa agattataaa ttagatccaa acgaaatcac
      661 actgatggtc gtagaggcaa tgccgacgat cttgaatatg ttggatcgaa atgatgctgc
      721 aaaagctgaa cgctatttgc gtaagcacaa cgtggaatta ttattgaatg caccaatcgt
      781 agaagtggct gctgatcata tcaaattgaa agacggacgc aacattccga ctcacacact
      841 gatttggaca gctggtgtca aaggaacttc tgacgctgct gactttggat tagaatcagc
      901 acgcggacag cggttgatcg ccaatgaata tatgcaggca aaaggctacg aagataaaaa
      961 catctatatc attggtgatt tagtctttta tgaagaattc ccaggaacac cgacaccgca
     1021 gatcgttcaa gctgctgaac aaacaggaca cacagccgca gcgaatatcg tggcttcgat
     1081 caaaggcgga gaaaagcata aatttaaagg aaactatcaa ggatttatgg tttctattgg
     1141 tgccaaatgg ggggtagcta acctatttga taagatccat ttaagcggat ttttagctat
     1201 cgtaatgaaa catatcgtga atttgaaata cttctttgat attcgttctg gttattatat
     1261 gttccaatat ttaatgcatg agtttttcca tatcaaagat gatcgcaacg tgactcgagg
     1321 acattcttct cgttacggca atgtcttatg gagtgtgcca cttcgtattt tctatggttt
     1381 agtttggttc gttgaagctt ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag
     1441 tacttggttt ggtgaaggat catggttcac tggaaacagt gtgttccctt ggacatggga
     1501 atacaatgta acagatgttg ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc
     1561 cagcggagca ggggcaagtg ccggagaagc agcgactcaa gctgcacatt ttggtttgag
     1621 ttatgcatac ggtgaagaac caatggctgt gattgagaag tcaccagact ggttcacaaa
     1681 gatcatgcaa gtgatcatgc caaacaatga tgtagcaatg ttcttccaaa aattcatggt
     1741 attctttgaa attgccttgg cattagcatt gattgctgga ttattcacat ggctatgcag
     1801 tgcgacaacg attgtattgg tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa
     1861 catttggttc atttttgttg ctttcgcatt gatgaacggt tccggacgtg cgattgggtt
     1921 ggatcgctgg gtaattccat ggctacaacg tacactaggt aattggtggt acggtagacc
     1981 aagttcccag tacggcaaga aataaataga taaagaacgt tatttgaatg caagatttca
     2041 atagaaaggg gctgagacaa accgtcacag cctccttttt atgtaaaatt aaaggaataa
     2101 gatgcccgag ataagcctcc atgagcaaga cacaggtgtc acaacctctg ttacaacctc
     2161 ttgttata
//
LOCUS       DADOOS010000232         2118 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.240, whole genome shotgun sequence.
ACCESSION   DADOOS010000232 DADOOS010000000
VERSION     DADOOS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.240"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            15..>2118
                     /gene="acm"
                     /locus_tag="J8C37_002807"
     CDS             15..>2118
                     /gene="acm"
                     /locus_tag="J8C37_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAZ5306200.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVI"
BASE COUNT          837 a          343 c          412 g          526 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatca
//
LOCUS       DADOOS010000233         2105 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.241, whole genome shotgun sequence.
ACCESSION   DADOOS010000233 DADOOS010000000
VERSION     DADOOS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.241"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            188..391
                     /locus_tag="J8C37_002808"
     CDS             188..391
                     /locus_tag="J8C37_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306201.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="J8C37_002809"
     CDS             602..1186
                     /locus_tag="J8C37_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306202.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(1375..2055)
                     /locus_tag="J8C37_002810"
     CDS             complement(1375..2055)
                     /locus_tag="J8C37_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAZ5306203.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          686 a          337 c          384 g          698 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt taatattaaa
     1321 ggttctgttg caaagtttta aataaagaat aaaatccctt acggtatcta tgatttaagc
     1381 tgggattccc aataatacct tgatttcagt acagaccgaa aacccgaaga gagtgccttc
     1441 ttttcgggtt ttcttatata atcctcgaat ggcttccatg cctttaatcg tggtagaggc
     1501 agtgcgtaaa cttcgataga atttattgcg tctctttact ggacgatggt cttgttcaat
     1561 caaattattc aggtatttaa tggtacgatg ttctgtccct tgataaaagc cgtattcttt
     1621 tagtttctta aaggcacttg taatagaggg ggctttatct gtgactacaa ccttcggttc
     1681 atcaaactgc ttcactaacc gcttaagaaa agcataggct gcttgtgtgt cccgtttttt
     1741 acgtaaccaa atatccaagg ttaaaccatc tgcatcgatg gctcgataca aataatgcca
     1801 ttttccttta attttgatgt acgtttcatc cattttccat gaataaaagg attttttatt
     1861 tttctttttc caaatttgat agagtagttt gccatattct tgcacccaac gataaatcgt
     1921 cgtatgagaa acgttaatgc cacgatcata taagatttct tgaacttcac gatagctaag
     1981 gttataacga agatagtagc ccacggctac aataatcaca tcctgctgaa attgctttcc
     2041 tttaaaatga ttcatcgtca ttcctcctgc tatctttttc tattattcta ccttatttga
     2101 tagta
//
LOCUS       DADOOS010000234         2104 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.242, whole genome shotgun sequence.
ACCESSION   DADOOS010000234 DADOOS010000000
VERSION     DADOOS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.242"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            169..1539
                     /locus_tag="J8C37_002811"
     CDS             169..1539
                     /locus_tag="J8C37_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAZ5306204.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="J8C37_002812"
     CDS             1560..1844
                     /locus_tag="J8C37_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306205.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DADOOS010000235         2090 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.243, whole genome shotgun sequence.
ACCESSION   DADOOS010000235 DADOOS010000000
VERSION     DADOOS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.243"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..914)
                     /locus_tag="J8C37_002813"
     CDS             complement(<1..914)
                     /locus_tag="J8C37_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAZ5306206.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="J8C37_002814"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="J8C37_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAZ5306207.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          700 a          373 c          407 g          610 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt
//
LOCUS       DADOOS010000236         2070 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.244, whole genome shotgun sequence.
ACCESSION   DADOOS010000236 DADOOS010000000
VERSION     DADOOS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.244"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            122..640
                     /locus_tag="J8C37_002815"
     CDS             122..640
                     /locus_tag="J8C37_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAZ5306208.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            647..1159
                     /locus_tag="J8C37_002816"
     CDS             647..1159
                     /locus_tag="J8C37_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306209.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1430..2053
                     /locus_tag="J8C37_002817"
     CDS             1430..2053
                     /locus_tag="J8C37_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306210.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          817 a          249 c          332 g          672 t
ORIGIN      
        1 tttatttaca tgccatccgc tttgaacgga ctacactggg catgtgacag catgcccttt
       61 catttatatt atatctcatt cttttatata atgaaagtaa ataaatcatg gaggataaaa
      121 tatggataaa tttaatagat taacattaat aaatcagttt gaaattttaa aagctttaaa
      181 tcctaacgaa gaaaaatact atgctgaaaa aatagaaata ctaacagaag gttacgaata
      241 tcactatagt gaaatatttg aaaatatttc tgatccattg ccagaagaag attcgcggtt
      301 tgttctagac atattaaata tgtatagaga tattactttt agtaaaaata aattaaaaga
      361 tataaattca attgataatt attatataca atttaaaggt tttgacttta atagctctac
      421 cgaatttaag ttagcacttt atgcacaatt ttttattgaa aaattaaata ggtttcaaga
      481 aatagttgaa gatagtgatt ttaacacttt taatagccat aaaccgatga gaggtactta
      541 tataaaattt ctcgaaaatt ataatgattt aaaatctacc aataattatc aatttggaaa
      601 cttgtcttat gagatgataa gtaaagttct aagtttgtga tgataaatga aattaaatag
      661 tataaatgat ttatttgcta ttataggtgt tattgctaac atactttttt cgtgtttgaa
      721 ttttagattt actcaaaaga acaaaaagaa caaactcagt atagaacaaa tgaaaaaaga
      781 cattgaaact gatttaaaaa atgaccataa agaaagaaga aaaattgaag aatatacttc
      841 tttaatggaa aagtgtaaag caactgatgc aatttttagc tttttaagaa aaagtgatga
      901 gataatcaca cgtgaaaata tagatttaga aaatgagatt agtaaacttg aatctatctt
      961 ttccgatata aattctctta agtatttaat cgtcgaagaa aattcggata gctttttaga
     1021 atttgaaaaa tatctaacag atcttataga taatagaaat aatgaattaa tgagacaaaa
     1081 cattgcaaga ttccttacta aattagtttc tgaatggaaa agtattattt ctcaaaatat
     1141 ggataaatta agatattaag gatgttagtt ttaaaataaa ctaatgtcct tatttttgtg
     1201 ttcacatttc tgttacctaa gaaatactta aaggaggtga tgtagccagc aacgactaca
     1261 acttgaaccc gctatctaca gtttatttgt tgtttctttt gggtatactc taatggtaca
     1321 aatccatatt tgcttaaagc gttgattttt cagcggtttt tctttattgc aaagtgtacc
     1381 ctaagacctg tatactaata gtaaatagat tggataggaa tgatggcaaa tgtatttagc
     1441 ttatttaaga gtttcttccg acgatcaaag tttagctcgg caacataagc taattgaaaa
     1501 ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa attttcgagg aaaaagtctc
     1561 tggaaaaaat attgaaaaca gaccagagct gcaagagctt atttctttca tacgtgaacg
     1621 agatacgatt attattccta gtcttgatcg attagcaaga aatagtaaag atacaaaaga
     1681 cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa atattagacc taccgtcgtt
     1741 taatggggta actgaccctg cattaagaga cttgctaaca aatttagtca tagaagtgtt
     1801 tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag cgtcaaaaac aaggcattga
     1861 aattgcaaag aaaaatggtc gttatactgg taaaaagatt caataccatg ccaatgcaac
     1921 aggaaaaaac aaattgattt acgatgaaat tgttgatggt ctgaagaaaa aagaaactat
     1981 ttctaaaata gcaaagaacg caggagttac aagaaaaacc gtttatcgag taaaaagaga
     2041 tttaggaatg taatatgttc cagttagaaa
//
LOCUS       DADOOS010000237         2056 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.245, whole genome shotgun sequence.
ACCESSION   DADOOS010000237 DADOOS010000000
VERSION     DADOOS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.245"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(587..952)
                     /locus_tag="J8C37_002818"
     CDS             complement(587..952)
                     /locus_tag="J8C37_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306211.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1028..1273)
                     /locus_tag="J8C37_002819"
     CDS             complement(1028..1273)
                     /locus_tag="J8C37_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5306212.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1343..1597)
                     /locus_tag="J8C37_002820"
     CDS             complement(1343..1597)
                     /locus_tag="J8C37_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306213.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1761..1973)
                     /locus_tag="J8C37_002821"
     CDS             complement(1761..1973)
                     /locus_tag="J8C37_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306214.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          758 a          352 c          366 g          580 t
ORIGIN      
        1 ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat
       61 tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt tactatcaat
      121 attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac tgatcacaaa
      181 atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg actacagagg
      241 cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc gatgtagtaa
      301 tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct
      361 gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg
      421 ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt ttagttttgc
      481 acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta agatggttaa
      541 agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag gtaagataaa
      601 ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg tgttccggat
      661 aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat gatttgccac
      721 gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc atttcatcca
      781 tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca taatccacag
      841 tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga tgttgtccta
      901 aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc atagaaatgt
      961 ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc accaaggctg
     1021 gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa
     1081 tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct tctccttctt
     1141 cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct acttgagata
     1201 aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt aaaatagctt
     1261 cttcgatact catttaatca cctttcatta tagtagaata gttataatga ctatagaaag
     1321 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
     1381 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
     1441 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
     1501 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
     1561 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
     1621 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
     1681 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
     1741 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
     1801 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
     1861 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
     1921 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
     1981 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
     2041 tcgaataaaa tcgcaa
//
LOCUS       DADOOS010000238         2003 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.246, whole genome shotgun sequence.
ACCESSION   DADOOS010000238 DADOOS010000000
VERSION     DADOOS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.246"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..378
                     /locus_tag="J8C37_002822"
     CDS             <1..378
                     /locus_tag="J8C37_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAZ5306215.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="J8C37_002823"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="J8C37_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAZ5306216.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="J8C37_002824"
     CDS             1859..>2003
                     /locus_tag="J8C37_002824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAZ5306217.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DADOOS010000239         2002 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.247, whole genome shotgun sequence.
ACCESSION   DADOOS010000239 DADOOS010000000
VERSION     DADOOS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.247"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            617..949
                     /locus_tag="J8C37_002825"
     CDS             617..949
                     /locus_tag="J8C37_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAZ5306218.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1568..1909
                     /locus_tag="J8C37_002826"
     CDS             1568..1909
                     /locus_tag="J8C37_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAZ5306219.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          692 a          271 c          423 g          616 t
ORIGIN      
        1 cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga gataatttca
       61 ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg aatattattc
      121 gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt gattttcgtt
      181 ctgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg
      241 aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat ttggccctta
      301 atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata acagatattc
      361 aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa
      421 tctatgccta agcacaatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg
      481 ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga
      541 aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa
      601 ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta
      661 cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa
      721 aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat
      781 ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct
      841 atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa
      901 tggagtcttg cgttgtaccg caaaagggat ggggcgtttg atcggctgaa ttgatcattc
      961 tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaagg
     1021 aacttagagt cactagaaaa ataaggagta gacttgatct atcaacaatt cccttcgggg
     1081 agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg
     1141 cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt aaacaagagg
     1201 gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca gctactaagg
     1261 gatctagact ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga
     1321 cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttgatta
     1381 ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga cgccataaac
     1441 gagtcgaaat tttttatgta tttttaagta aacgaaaaac gataaaatat gaacaagtcc
     1501 tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg taatggagga
     1561 atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta ttaaggtttc
     1621 tttccaacgt tgggattaca aaggatttgt gacgtttaga agaggcggaa attgtaaagg
     1681 attagatgtt ttagcattag acgaggatga tttatacgat caaacattga ctgataatcc
     1741 aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct
     1801 tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta
     1861 tattgtcagc tttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga
     1921 ataaatttta cagataaagg cttagaagga gtcacgatga ttaataaaaa ggtagtatta
     1981 ccttaggcat tgagtaagga at
//
LOCUS       DADOOS010000240         1982 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.248, whole genome shotgun sequence.
ACCESSION   DADOOS010000240 DADOOS010000000
VERSION     DADOOS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.248"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="J8C37_002827"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="J8C37_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAZ5306220.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="J8C37_002828"
     CDS             852..1040
                     /locus_tag="J8C37_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAZ5306221.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="J8C37_002829"
     CDS             1053..1610
                     /locus_tag="J8C37_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAZ5306222.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="J8C37_002830"
     CDS             1642..1884
                     /locus_tag="J8C37_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAZ5306223.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          305 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 tt
//
LOCUS       DADOOS010000241         1962 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.249, whole genome shotgun sequence.
ACCESSION   DADOOS010000241 DADOOS010000000
VERSION     DADOOS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.249"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            44..117
                     /locus_tag="J8C37_002831"
     tRNA            44..117
                     /locus_tag="J8C37_002831"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Ile,seq:cat)
     gene            complement(502..999)
                     /locus_tag="J8C37_002832"
     CDS             complement(502..999)
                     /locus_tag="J8C37_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAZ5306224.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1039..1848)
                     /locus_tag="J8C37_002833"
     CDS             complement(1039..1848)
                     /locus_tag="J8C37_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAZ5306225.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          569 a          363 c          346 g          684 t
ORIGIN      
        1 gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt tagctcagtt
       61 ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa
      121 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
      181 ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag
      241 gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt
      301 tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta
      361 agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga
      421 tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat
      481 cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag
      541 ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc
      601 tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc
      661 aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat
      721 ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc
      781 ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag
      841 ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa
      901 tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg
      961 atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat
     1021 atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc
     1081 attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc
     1141 aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat
     1201 gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt
     1261 gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc
     1321 tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa
     1381 tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt
     1441 ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt
     1501 cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata
     1561 gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat
     1621 ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat
     1681 tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac
     1741 gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc
     1801 atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta
     1861 ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat
     1921 gaaactaact tgctttgtca ttatgaattt tttcaaaaaa ca
//
LOCUS       DADOOS010000242         1955 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.250, whole genome shotgun sequence.
ACCESSION   DADOOS010000242 DADOOS010000000
VERSION     DADOOS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.250"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            103..1569
                     /locus_tag="J8C37_002834"
     CDS             103..1569
                     /locus_tag="J8C37_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAZ5306226.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          609 a          334 c          397 g          615 t
ORIGIN      
        1 ataaaagaat acacacataa aataacactg tcttgtaagt ttgttaaaaa ttgaatatga
       61 ttacatctgg ataacaaatt tttatcataa aggagagaac gaatgaacaa caaacaatta
      121 cgctggttta ctgttgcgtt aattgcattt aacatggttt ggggcatggg gaatgtagtt
      181 aataactatg ctcagcaagg agtatcagtt gtcacctctt ggctattgat tttggccatt
      241 tattttattc catacgcatt gatcgttgga caattaggat cagccttcaa ggatagtaaa
      301 ggaggcgtca gttcatgggt cgaaaatacg acatctaata aacgactggc atactatgcc
      361 gcttggactt actgggtcgt ccatattcct tatttggcac aaaagcctca agcaattctg
      421 attgctggtg gttgggcagt ccaaggaaat ggaaatatcg taaatacgat gtcagtacaa
      481 atggtagcag ttatttcact tattatattt cttgcctttt tatatttatc aacaaaagga
      541 ctttctactt tgaaagtgat tggcggactg gccggaacag cgatgttcgt catgtcgatc
      601 ttatttattc ttttagctgt aacggcaccg ttcatcaatc caacgatgga atttgccaca
      661 ccacatatga atcaagtgaa aacatatatt ccaacatttg atttcagtta tttcacaacc
      721 gtttcgatgc tagtatttgc agttggaggg gcggaaaaaa tatcccctta tgtcaactct
      781 acaagaaatc ctgcaaagga atttcctaaa gggatgatct tcttagcagc aatggtaggg
      841 atttgtgctg tattaggctc agtcgcaatg ggtatgctgt ttgctagtga taatattcct
      901 aaagacttga tggcaaatgg ggcatacagt gcttttcaaa ttttaggtaa ttactatggt
      961 atcggtaact cattgatgat catttacgca ttgacaaaca tgattggaca aatctccgca
     1021 ttagcctttt caattgatgc accactaaaa atcttgttag cagatgcgga tccagaattt
     1081 gtaccaaact ggctacgtaa acgtaatcgt aaaggtacat tggtgaatgg ttatcttctg
     1141 acaggtatac tagtaggaat cattattttg ttgccgattt ttggtatcaa agatatgaat
     1201 gaattagtaa aatggttgac gaacttgaac tcagttgtca tgccaatgcg ttacctatgg
     1261 gtgttcttgg catatatgat gttgaataaa gcttataaga aattcaattc tgaatataaa
     1321 tttgttaaaa atccaaaaat cggttttatc tttggtgctt ggtgctttgc cttcactgcg
     1381 tttgcatgta tcctaggaat ggtgccaaaa gtagattata ctgctgatcc taaagcatgg
     1441 tggttccaat tatttacgaa tatcatcaca ccaatcgtct tgatcttatt aggtatgatc
     1501 ttgccagcaa ttgctcgtcg agacaaaaac aaaaaaatca ctgaaccaga aacagtggtt
     1561 cagccttaaa atagaaatac ttgttacaag aggttgtgac agaagtggtc agcttcaaga
     1621 aataagaagg aatttacgaa aattgcttct caaatttttg tgaagtttcg gcttgttttt
     1681 cgaagaagct acttctggaa caccgtgaat cagaggttgt gaaaaagctg tcctgcatca
     1741 agtaataaga acagccaaac aaaaatagcc cctcatattt ttcgtttggc tgggcttatt
     1801 accgcagatg caagctttgg aacaccgttt aaggaagagg ttgtaaaatc agcgatttga
     1861 cctgagtatt agagaaacaa acggaaaaat agctcctcat attttgccgc tttttgagct
     1921 aatgctgaag gtcagctgat tgaacaccat ttatt
//
LOCUS       DADOOS010000243         1918 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.251, whole genome shotgun sequence.
ACCESSION   DADOOS010000243 DADOOS010000000
VERSION     DADOOS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.251"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(243..464)
                     /locus_tag="J8C37_002835"
     CDS             complement(243..464)
                     /locus_tag="J8C37_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306227.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="J8C37_002836"
     CDS             complement(562..807)
                     /locus_tag="J8C37_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306228.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="J8C37_002837"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="J8C37_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          642 a          335 c          281 g          660 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcat
//
LOCUS       DADOOS010000244         1884 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.252, whole genome shotgun sequence.
ACCESSION   DADOOS010000244 DADOOS010000000
VERSION     DADOOS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.252"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..493
                     /locus_tag="J8C37_002838"
     CDS             <1..493
                     /locus_tag="J8C37_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAZ5306229.1"
                     /translation="FLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVYT
                     SNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYL
                     EFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLS
                     ILI"
     gene            495..>1884
                     /locus_tag="J8C37_002839"
     CDS             495..>1884
                     /locus_tag="J8C37_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAZ5306230.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDST"
BASE COUNT          693 a          234 c          324 g          633 t
ORIGIN      
        1 gtttttagta atgaaaatta ttgatgctat caagatagag ttttttacta tattttttgc
       61 gacatcctta tatattgtat ttcgaatgtt gccggtcgta atgatctcat ggttgttggt
      121 tagtgtctat actagcaatg aattattatc ctctttagaa aaaatacatt taccgaaaaa
      181 aataatgtta agtattacag tagtgatcag attctttccg acttatcgtt ctgaaataag
      241 aatgattaaa gaatctttaa aaatgagaaa cattcgcttg tctttttttc agcctctgca
      301 atatttggaa tttctgttag ttcccgttct tatacgtgca actatgattg ctgaagaaat
      361 gaccgctaat gcaataacaa aggggataga atctcctgtg aaaagaagtt cttactataa
      421 aacaaacatg agcttattag actatatcat cctgttttcc gttactattt ttttcttaag
      481 tattttgatt tgaggtgaaa gaattgtttg aatttaacaa tgtttctttt tcatataagg
      541 atgttaagga acaagcgctt gttgacctta atttttcaat aaaaaaaggc agtcttacat
      601 tgattactgg ttcttctggg agtgggaaat ccacactatt aaagttaatg aatggactta
      661 ttccaagact ttatgacgga aaattggttg gggaaatttt atttgaaaat aactcgctac
      721 aatccttaac acaagaaagt atatcaaaaa atattggcta cgtaagtcaa gatcctagag
      781 gacaattctt tacaacaaat acaacgagtg agttagtatt ttcgatggaa aactttggat
      841 ttacaaaaga gcatatggat aaaagaatca atgaattagc tgaattatta gatctttcaa
      901 atatattaga taaaaacatt ttttcgctat caagtggtga acggcagaaa atttctattg
      961 gatgtagttt atcgctaaac tctaaattaa ttatattgga tgaaccgtct tcaaatttgg
     1021 acttcaaagc gactaagaaa cttgctgact tactttgtga tttaaaaaat aaaggattta
     1081 ctattgttat agctgaacat cgtttttact atttgaaaga attaattgat caagttctat
     1141 ttgttaaaaa tagaactgta aaaactacaa ctattcagtt gttaaaaact gaaaacaata
     1201 acgcattaag gaaatttgac atttttaata gttcgattga agttccctat atcgaaaagg
     1261 taagtgcaga tgtattaaaa gttgaagaaa ttacttatga aaatattcta aaagacataa
     1321 attttgatgt caaagttgga gatgttattg gtttggtagg gaaaaacggt gtggggaaaa
     1381 caaccttact aagaatactc atgaagatta ttcgccctac caaaggaaag ataatcgaaa
     1441 ataaaaaaaa tttgtcatcg ccgtttttag taatgcaaga aatggattac caatttttca
     1501 cagaatcagt gaggagcgag ttagccctgg ggaatgaaat agttagtaaa gatcagcaag
     1561 aaaaaatttt aaaaaaaatg gaactttata aaatgaaaga tcaattaccg tttgatttat
     1621 ccggtggaga aaaacaaaga ttattggttt ctatagcttc attgtcaaaa actaatttat
     1681 ttctatttga tgaacctaca agtgggttgg attatattaa tatggaaagg attgctgacc
     1741 ttattaaaga cttaaaagaa aagggtgcag tagttatagc tactcatgat ccggaatttc
     1801 tttataaaac atgcaataga attatttatt taaaaaatgg tggaataaaa aaagatataa
     1861 aacttagagt agaagattca actt
//
LOCUS       DADOOS010000245         1878 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.253, whole genome shotgun sequence.
ACCESSION   DADOOS010000245 DADOOS010000000
VERSION     DADOOS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.253"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..426)
                     /locus_tag="J8C37_002840"
     CDS             complement(<1..426)
                     /locus_tag="J8C37_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAZ5306231.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="J8C37_002841"
     CDS             complement(535..1566)
                     /locus_tag="J8C37_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAZ5306232.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          578 a          316 c          325 g          659 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctc
//
LOCUS       DADOOS010000246         1829 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.254, whole genome shotgun sequence.
ACCESSION   DADOOS010000246 DADOOS010000000
VERSION     DADOOS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.254"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            127..693
                     /locus_tag="J8C37_002842"
     CDS             127..693
                     /locus_tag="J8C37_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5306233.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            838..925
                     /locus_tag="J8C37_002843"
     tRNA            838..925
                     /locus_tag="J8C37_002843"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Ser,seq:cga)
     gene            1259..>1829
                     /locus_tag="J8C37_002844"
     CDS             1259..>1829
                     /locus_tag="J8C37_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAZ5306234.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          641 a          276 c          355 g          557 t
ORIGIN      
        1 aaaaagttaa agaagactga aaaaaaattt aaaatataac gttttgagta aaatgtttgc
       61 aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag aagtggggga
      121 gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc ttatgatgtt
      181 gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa gatggggatt
      241 tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa tacgttgctg
      301 gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac tggtgataaa
      361 atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag tctttacatg
      421 gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc atacaattat
      481 ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga atatgtcgat
      541 gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat gtcatcaaac
      601 atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat tgactcgatt
      661 ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa tttgatcggt
      721 aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga tttctgttat
      781 ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga
      841 aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta ataccggcgc
      901 gcaggttcaa atcctgtacc ttccttatat caacgattag aggttgtgaa gaagctgtcc
      961 tgcatcaagt aataagaacg gccaaacaaa aatagctttt cctatttttt cgtttgattg
     1021 ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaataag gggctgtgac
     1081 aagacttttg tcacagcccc ttattgttgt acttttaacc tacaatattt tttgattccc
     1141 cctgagtact attttttata aatgctagta aatcaatctt ctttggcctt tttttgataa
     1201 aaaaaatact agatgtaaga taaaagtgta aaaacgattt gttttaggag gggaaacgat
     1261 gtgtaccgca atcacttatg caacgaaaga ccattatttt ggaaggaatt ttgattatga
     1321 gatgtcttat aatgaagtgg tcactattac tccaagaaat tatcgtttcg attttagaaa
     1381 ggtaaaaaat ttagataaac attatgcaat gataggaata gcagctgggg tatccaacta
     1441 tccactctat tatgaggcta caaatgaaaa agggttgagt atggcaggac tgaattttcc
     1501 tggcaatgct gattataaag aattacaaga aggaaaagat aacgttgcac catttgagtt
     1561 tattccatgg atactagggc aatgctcaac catagacgaa gctaaagaat tattggctac
     1621 tatcaatcta gtcaatatcg attttagtga aaaactaccc ttgtcacctt tacactggtt
     1681 attagctgat aaagaaaaat cgatagtaat tgaaagtatg aaagatggac ttcatttata
     1741 tgataatcct gtcggtgtgt tgacaaataa tccgccattt gattatcaat tatttaatct
     1801 gaacaattat cgctcgctat caaatggaa
//
LOCUS       DADOOS010000247         1817 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.255, whole genome shotgun sequence.
ACCESSION   DADOOS010000247 DADOOS010000000
VERSION     DADOOS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.255"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..216
                     /locus_tag="J8C37_002845"
     CDS             <1..216
                     /locus_tag="J8C37_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAZ5306235.1"
                     /translation="TIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGY
                     DKKVLSMYKNVFFEMLERIIQLENEKE"
     gene            329..544
                     /locus_tag="J8C37_002846"
     CDS             329..544
                     /locus_tag="J8C37_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAZ5306236.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            561..833
                     /locus_tag="J8C37_002847"
     CDS             561..833
                     /locus_tag="J8C37_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAZ5306237.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            835..1698
                     /locus_tag="J8C37_002848"
     CDS             835..1698
                     /locus_tag="J8C37_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAZ5306238.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT          736 a          285 c          330 g          466 t
ORIGIN      
        1 acgataaaat caaacctgga agaactgtat aagcaacata aagaagataa cttggttttc
       61 caaaatatta tcaagcgaag taataaagta agtacctggt ctaaaaacgg cattacagag
      121 cataaaggct atgacaaaaa agttttatcc atgtataaga acgtattttt tgaaatgctt
      181 gagcgaatta ttcaattaga aaacgaaaaa gaatagaatc acaaatcaca agtgattaat
      241 cacaaatcac ttgtgatttg tgattaatca cttgtgattt gtgattgttg atgataaaat
      301 aagagtaaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaagc
      361 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      421 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      481 aaaagaagtt atggatcaag ccttagaaga atatatacgg aaatatttac ctgacaaact
      541 ttaaaaggag tgaaaatgaa atggcagtta cgtatgaaaa aacatttgaa atagagatca
      601 ttaacgaatt atcggcaagc gtttataatc gagtattaaa ctatgttttg aaccatgaat
      661 taaataaaaa tgactctcaa ttattggaag tcaatttatt aaaccaatta aagcttgcaa
      721 aacgtgtaaa tctttttgat tattctttag aagaattaca agccgttcat gagtattggc
      781 ggtcaatgaa tcgttactca aaacaagttt tgaataaaga gaaagtggct taatatggca
      841 aatatagtca attttactga caaacaattt gagaatcgct taaatgataa tttagaagaa
      901 ttgattcaag gaaaaaaagc ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca
      961 gggtcaggga aaaccagttt gcgatcagca atttttgaag aaacacaagg gaatgttatt
     1021 gtcattgata atgatacctt taaacaacag caccctaatt ttgatgaact agtgaaactt
     1081 tatgaaaaag acgtagtaaa acacgttacc ccttattcta atcgcatgac agaagcgatc
     1141 ataagccgtt tgagcgatca agggtataat ttggtgatcg aaggtacagg acgaacaaca
     1201 gacgttccta ttcaaaccgc aacaatgctt caagccaaag gttatgaaac aaaaatgtat
     1261 gtcatggcag tacctaaaat caactcatat ttaggaacaa ttgaacgata tgaaaccatg
     1321 tatgcagatg atccaatgac agccagggca acaccaaaac aagcgcatga tattgttgtc
     1381 aaaaacttac cgaccaattt agaaaccctt cataaaacgg gcttatttag cgatataagg
     1441 ctatataaca gagaaggagt aaaactctat tcaagtttgg aaacaccttc tattagtcca
     1501 aaagaaacct tagaaaaaga attgaatcgt aaagtatcag ggaaagaaat tcaaccgact
     1561 ttagaacgaa tagagcaaaa aatggttcta aataaacacc aagagacacc tgaatttaaa
     1621 gcaattcaac aaaaattgga aagcttgcag ccacctacac caccaatacc caaaacacct
     1681 aaacttccag gtatttaaac cttaaaaaag gaaaagagta gttaccaaaa aacggtaact
     1741 actctttttt tataaaaaca tttcctcatg ttggggtccc gagcgcctac gaggaatttg
     1801 tatcgataag aaataga
//
LOCUS       DADOOS010000248         1815 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.256, whole genome shotgun sequence.
ACCESSION   DADOOS010000248 DADOOS010000000
VERSION     DADOOS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.256"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            177..374
                     /locus_tag="J8C37_002849"
     CDS             177..374
                     /locus_tag="J8C37_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306239.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            480..962
                     /locus_tag="J8C37_002850"
     CDS             480..962
                     /locus_tag="J8C37_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306240.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1020..1616)
                     /locus_tag="J8C37_002851"
     CDS             complement(1020..1616)
                     /locus_tag="J8C37_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ5306241.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          562 a          276 c          364 g          613 t
ORIGIN      
        1 tttttgaaaa aaattcttct aaatacaaca gctgttttaa ttgtgaaaac gaaaacagta
       61 cgctataatg cctgtaaggt tgatttttaa ctgtaaataa aaatcaatga gttaaatctc
      121 caaagaaata gcagtggtaa tgtggagaaa agttccagca gtttaggagg attttgatga
      181 cggaagaaat gattaattta ggagaacaat atgcatgcaa accaattggt tttacaaaaa
      241 cggtcattgg tgaagttgta tcaaaaatga caaactgcgc cgtggtcaaa gttgcccaat
      301 gtgctgcaga ggaccaagaa ttattagatg aaaaagcaag catggttgtt gcaaaatatg
      361 atacatttga ataaagaaaa caattggacg tgcggcgtgc tgcaagtcca atttctttga
      421 gaaggtaaat cagtattctt ttcctatttt ctggagaaga aaaagcgagg cgagttgtca
      481 tggtaacatt ctttacggtt ctattgtttt tattcgttgt tgtgatgttt ttatttcttt
      541 ggctggttcg aaaagaaatt atttatcgga ctgtgcgaaa tcgatgggtc tacttggtga
      601 tacccttctt agtgtcacta gtcatttggt atacactcat cagtcatcct acagcagatg
      661 aattggcaaa agggatcttg tcagcaatga tttttattag ttttttgttg gacagtagag
      721 gaatcacaga agaaggactt gttttgaata gctttgacaa aaaaggtgtt cctttttctg
      781 agatcaataa aattgtatta tatcagccaa aaggttctaa aatcgtaaaa atgaattttt
      841 tccgaaacgg atggcgagga ccgatgctga aattttctgc atcattagaa gaattagttc
      901 catttttgtc ccaacgctta aacgatgagg cagagattga tattatgatt gatccagaat
      961 aaaaacagag gttgcgacaa gatgtttgtc acaacctctg ttttttattt caaataaaat
     1021 tatccaaata cgcggaagcg atcggcatcg ttcatgtatt ctttttcccc agccggtgtt
     1081 tctggtgaac cgaatactaa ttgcgaacgt agtttccagt ttgaagggat attccattct
     1141 tttgcaactg cttcgtcaat aactgggttg taatgttgca agtttgcgcc caatccttta
     1201 tcaactaatg ctacccaagt attagctgtt gcgattccgt ttgattgttc tgaccaatct
     1261 gggaagttgt ctgcatataa ttcaaattgt tcttgtagac cttttaccac gtcagtatct
     1321 ttgaagaata atactgtacc ataaccattt ttgaaaccag ctaatttgtt ttgtgtattt
     1381 gggaaagctt ctgctggagt caatgggcga agtgcttctt cagtgatttc ccaaagtttt
     1441 tcatgtgcat ctccgaaaag gataactgcg cgtgttgatt gagcattgaa agcagttggg
     1501 ctttctttaa ttgcttcttt gatcaaagtt gttaattctt cattagaaag tgaaacatta
     1561 cgtcctagat gatagattga acggcggttt ttcaaagtgt cagtaaaagt tgtcataata
     1621 aaatttctcc tttgatttgc gttttgcatt tttttaattg attacgaaat gaagtatagc
     1681 accttacaaa aagtaagtaa acagatatgc tcacaaaaaa taagcgaagt tcaaaaaaca
     1741 attttaataa gaaaaatcgt tatgtaagga ttttcttagt tatcgctttc attgttgtgt
     1801 tttaggaatt tttgc
//
LOCUS       DADOOS010000249         1769 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.257, whole genome shotgun sequence.
ACCESSION   DADOOS010000249 DADOOS010000000
VERSION     DADOOS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.257"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            62..676
                     /locus_tag="J8C37_002852"
     CDS             62..676
                     /locus_tag="J8C37_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306242.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..1665)
                     /locus_tag="J8C37_002853"
     CDS             complement(841..1665)
                     /locus_tag="J8C37_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306243.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          588 a          303 c          267 g          611 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     1081 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     1141 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     1201 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     1261 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     1321 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     1381 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     1441 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     1501 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca
     1561 attactatct tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc
     1621 tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag caatattgct
     1681 aaaatcagct aatttttctt tagaataaaa aaaacattta tatcagaatc aataaattct
     1741 atttttttta ttactatatc gtcatcatt
//
LOCUS       DADOOS010000250         1731 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.258, whole genome shotgun sequence.
ACCESSION   DADOOS010000250 DADOOS010000000
VERSION     DADOOS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.258"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            143..802
                     /locus_tag="J8C37_002854"
     CDS             143..802
                     /locus_tag="J8C37_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAZ5306244.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            821..>1731
                     /locus_tag="J8C37_002855"
     CDS             821..>1731
                     /locus_tag="J8C37_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAZ5306245.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTI"
BASE COUNT          669 a          211 c          324 g          527 t
ORIGIN      
        1 gaggaagaaa cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat
       61 ttttataaaa agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa
      121 aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa
      181 caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta
      241 tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa
      301 cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt
      361 agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc
      421 tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa
      481 aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca
      541 agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac
      601 acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt
      661 gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat
      721 tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca
      781 tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt
      841 agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac
      901 aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct
      961 cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc
     1021 acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga
     1081 atataatata gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc
     1141 gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg
     1201 ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc
     1261 agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg
     1321 aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa
     1381 agatatacag ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca
     1441 aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat
     1501 gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc
     1561 aggaaaaaaa tatcaattaa aaccattatt atatataatt gatgaaatac taaatagttt
     1621 ctatcagaag agttattggg gatattcatt accaaactat atttcagcag ttcataatgc
     1681 tcatcctaat tatgcaagct atctgtcata taagaatact ttaacaattg a
//
LOCUS       DADOOS010000251         1709 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.259, whole genome shotgun sequence.
ACCESSION   DADOOS010000251 DADOOS010000000
VERSION     DADOOS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.259"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(149..526)
                     /locus_tag="J8C37_002856"
     CDS             complement(149..526)
                     /locus_tag="J8C37_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306246.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(826..1707)
                     /gene="galU"
                     /locus_tag="J8C37_002857"
     CDS             complement(826..1707)
                     /gene="galU"
                     /locus_tag="J8C37_002857"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAZ5306247.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          479 a          379 c          251 g          600 t
ORIGIN      
        1 ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga
       61 aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt
      121 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
      181 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
      241 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
      301 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
      361 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
      421 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
      481 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
      541 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
      601 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
      661 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
      721 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
      781 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
      841 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
      901 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
      961 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
     1021 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
     1081 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
     1141 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
     1201 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
     1261 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
     1321 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
     1381 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
     1441 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
     1501 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
     1561 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
     1621 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
     1681 aggaatgact gcttttttga ccttcactt
//
LOCUS       DADOOS010000252         1698 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.260, whole genome shotgun sequence.
ACCESSION   DADOOS010000252 DADOOS010000000
VERSION     DADOOS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.260"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..145)
                     /locus_tag="J8C37_002858"
     CDS             complement(<1..145)
                     /locus_tag="J8C37_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAZ5306248.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNM"
     gene            complement(142..795)
                     /locus_tag="J8C37_002859"
     CDS             complement(142..795)
                     /locus_tag="J8C37_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAZ5306249.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1083..>1698
                     /locus_tag="J8C37_002860"
     CDS             1083..>1698
                     /locus_tag="J8C37_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5306250.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLR"
BASE COUNT          543 a          310 c          234 g          611 t
ORIGIN      
        1 ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa
       61 ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt atgaacattt
      121 ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt
      181 agttatacag tctaattgta atttttttct aagttctttt atatatacat ctattactcg
      241 atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa
      301 cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg catttacatc
      361 tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata
      421 ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa
      481 gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat
      541 tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct
      601 aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa
      661 atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt
      721 atatccaaat tctgaaagat attcacttat tccctctctt atcatattat catcttcaac
      781 agttaatatt ttcatatata cctccactta agcctataca ctagatatta tacagcatat
      841 tatggctcta taatatctag tgttggtgac accttctgtt gccaatactt tttctttttt
      901 ctaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta taatattttg
      961 tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa
     1021 agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct
     1081 ctatggataa tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg
     1141 aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat
     1201 tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt
     1261 cctatacgac acgtactcag atattgaaag tcaaagaaaa gttgactatt ttagaattaa
     1321 aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag
     1381 ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg
     1441 aaaatcaatc acgtaaacta atcgctaaga agtacttcgt ctcggatggg acggtgacac
     1501 ggatcttgcg tgaagcaacg aagcattacc agccacgtat gaacttcctt ccgacggtgt
     1561 tgtgtatgga tgagttcaaa tcaatgaagt ctgtttctgg ttccatgagt tttatttgtg
     1621 ttgatgggac aacccatcag ctatttacga tccttgaaga tcgcagactt tacaaactaa
     1681 ctcaatactt cctgcgct
//
LOCUS       DADOOS010000253         1693 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.261, whole genome shotgun sequence.
ACCESSION   DADOOS010000253 DADOOS010000000
VERSION     DADOOS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.261"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            445..1575
                     /locus_tag="J8C37_002861"
     CDS             445..1575
                     /locus_tag="J8C37_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAZ5306251.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          487 a          274 c          350 g          582 t
ORIGIN      
        1 attctttcaa cttatttgtt atttttttct aaaaaatatt aaagattgtt aaatttcact
       61 atttcatttc aaattccatt gcctaatcat taggaaaagt ctatcatgaa ctcgtaaata
      121 gaatatagta ataaccttgt taggtgaggc tcctatacaa acataggctg ttgcccagaa
      181 acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga ttaaggcttt ttccaatagc
      241 taagaatgtt ttctttacgt tgtatagtgc taaaactcgg acgatgagat gatgctaggt
      301 cagaattctt gttcgaaaag gtttctgacc ttttctttat atttttgtga ctgacttatt
      361 tgatgagaca aactcgctcc tcacctgaca agtggattgc tgtgtgagga ggagatgcgg
      421 tgaaaaaaga tcgattggtg gattttgggt ccttatttga ctttgtccat gatcggtctt
      481 ttagaagtat acagtgccag ttcttatcgt ttattgcaag ccgatgaaaa tacaaaaagt
      541 ctattattgc gtcaactaat tttcatattt ttgagttgga gcgtgatctt cttagctcgt
      601 tcagtcaaac tacactattt acttcaccct aaaatagcag gatacggttt agccttatcg
      661 attttctttt tagtattagt aagaataggg atattcggtg tcactgtcaa cggcgcacaa
      721 cgttggatct ctctgtttgg cattcaattc cagccttctg aactggcaaa tctttttttg
      781 attttttatt taagctggtt ttttcgtgac ggaaatagta gcccaaaaga tctaaaaaaa
      841 ccattcctga ttacagtagg tataactttt ctgattttat ttcagccaaa gattgctgga
      901 gcattgatga tcctttcgat tgcgtgggtc atattttggg cagcggcggt tccatttaaa
      961 aaagggatct atctaatcgt tactttttct gcattgctga ttggagcagc aggcggggta
     1021 ttatatttag gaaataaagg ttggcttcca cagatgttta atcatgctta tgaaaggatc
     1081 gcgactttaa gagattcttt tatcgatagt catggtgcgg gctatcaaat gacccattcg
     1141 ttttatgcac tatataatgg tggcatattt gggcggggat taggaaatag tatcacaaaa
     1201 aaagggtatc ttccagagac agaaacagat tttatttttt cgattattac agaagagctt
     1261 gggctaatcg gtgcattgtg tgtgcttttc ttattgttct cattatgcat gagaatcttt
     1321 tgtttaagca gtcgctgtaa aaatcagcaa gctggattgt ttcttttagg attcggaact
     1381 ttgctattcg ttcagacgat aatgaacgtg ggaagtatcg cggggctgat gccaatgact
     1441 ggtgtgccat tgccatttgt tagctacgga ggaacaagtt atctgatcct ttctttagga
     1501 atcggaatca cgttgaatat ttcttcaaag atacaggccg aagagttggc gctctatcga
     1561 cctgaaaaac aataaagaga aactgagaaa ctctcagttt gaattatcat aaagtacaat
     1621 cttttatttt tcaagcgaga ggttctttta gaaagaggaa ttcgcaatga aagtatctca
     1681 aaaaatcata tta
//
LOCUS       DADOOS010000254         1685 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.262, whole genome shotgun sequence.
ACCESSION   DADOOS010000254 DADOOS010000000
VERSION     DADOOS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.262"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(141..1433)
                     /locus_tag="J8C37_002862"
     CDS             complement(141..1433)
                     /locus_tag="J8C37_002862"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAZ5306252.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          483 a          351 c          295 g          556 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaaaa gaggttgtaa cagaagtggt cagcttcaag aaataagaag
     1621 gaatgtacga aaattgcttc tcaaattttt gtgaaatttc gacttatttt tcgaagaagc
     1681 tactt
//
LOCUS       DADOOS010000255         1660 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.263, whole genome shotgun sequence.
ACCESSION   DADOOS010000255 DADOOS010000000
VERSION     DADOOS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.263"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            138..752
                     /locus_tag="J8C37_002863"
     CDS             138..752
                     /locus_tag="J8C37_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAZ5306253.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="J8C37_002864"
     CDS             complement(776..1126)
                     /locus_tag="J8C37_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAZ5306254.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="J8C37_002865"
     CDS             complement(1120..1383)
                     /locus_tag="J8C37_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAZ5306255.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          558 a          294 c          256 g          552 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa
//
LOCUS       DADOOS010000256         1657 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.264, whole genome shotgun sequence.
ACCESSION   DADOOS010000256 DADOOS010000000
VERSION     DADOOS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.264"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            206..544
                     /locus_tag="J8C37_002866"
     CDS             206..544
                     /locus_tag="J8C37_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306256.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="J8C37_002867"
     CDS             complement(749..1324)
                     /locus_tag="J8C37_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAZ5306257.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          519 a          334 c          279 g          525 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaa
//
LOCUS       DADOOS010000257         1655 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.265, whole genome shotgun sequence.
ACCESSION   DADOOS010000257 DADOOS010000000
VERSION     DADOOS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.265"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            1..399
                     /locus_tag="J8C37_002868"
                     /pseudo
     CDS             1..399
                     /locus_tag="J8C37_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            554..1129
                     /locus_tag="J8C37_002869"
     CDS             554..1129
                     /locus_tag="J8C37_002869"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAZ5306258.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1172..1339)
                     /locus_tag="J8C37_002870"
     CDS             complement(1172..1339)
                     /locus_tag="J8C37_002870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306259.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            1356..1538
                     /locus_tag="J8C37_002871"
                     /pseudo
     CDS             1356..1538
                     /locus_tag="J8C37_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          585 a          251 c          325 g          494 t
ORIGIN      
        1 ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga
       61 ccgtacggtc aagaccgagt atggggaact tcatctccag attccgcgcg accgcaatgg
      121 cgagttcaag caacagactg ttcctgctta tagacggacg aatgacacat tagaggagac
      181 cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa
      241 aatgtatggg catcactaca cgccccaaac catgtccaac atgactaaag tgctgactga
      301 agaagtaaat gcatttaaag ttagaactct aaatgataag tatgtcgcta ttttcatgat
      361 tccttcagtt tttcagatga tataccactc aaaaaacaat atgagagatg ttcaggaata
      421 ccatctctaa tggcttataa attggtttta gggatagtag atgttataga catatcaagg
      481 aataaacgag cagctgcata aaagcagatg gaattaagga ataattgaat tgtaaaagaa
      541 gggaataaat ttaatggcac aattatttta cagatatgga acaatgaata gtggaaagag
      601 tattgagcta ctgaaagtgg ctcataatta tgaagaacaa gggaagagag ttatcataat
      661 gacaagcgca atagatactc gatcaggatt tggaaaagtt gagagtcgta taggtgtttc
      721 gaaaccagct attgcaatta cttctgaaat ggatgtgtac ggagaattag tggagattat
      781 tgatcagtac ggaaaagttg attgtatttt ggtcgatgag tctcaatttt taagtagaga
      841 gaatattatt gatctatcac ttatcgtaat gaatgaggaa gtaccagtaa tttgctatgg
      901 attgaagaat gattttcaaa ataatctctt caagggatcc gaagcattat tgacatttgc
      961 tgataaaatt gaggaaatta aaactatttg ccatttttgc aataaaaagg ctgtaatgaa
     1021 tgctcgtatt gttgatggta ttccagttta ttcaggtgaa cagattgtag taggaggtaa
     1081 tgaagattac ctttcggtat gtcgcaacca ctactacaac gaagattgaa ataaaaaacg
     1141 tgtaatcaaa taattgatta cacgtttttt attattctgt taaaggttga ggatcagaaa
     1201 tttttaggta tctataatcc tcacctaaaa atccgttaaa acgaagtaaa acatcatgac
     1261 catttaaatc agttggaact aaaattggaa ttaatttatc aacagtagtt ttgttgtaaa
     1321 gttcaaaagt gttctccatt ccttctggtt ctacaatcga atctttcaat aaacaaatca
     1381 agaaatacag ccgcagaaaa gagcagtttc aaaatgaaga atcactagag cgtttcctag
     1441 tatccatctt tgatacatac aatcaaaaat tcttaaatag aagccataaa ggcttccaac
     1501 aagtgacgga tacattagct tcaatgttta ctgagtaacc cattattttg cagaaggacg
     1561 agttatttac acaaaattat tgacgctccc tgtttggagt ggtaaaaaga ttgagaaatt
     1621 gtttcattag ctattacgat tattattgta atata
//
LOCUS       DADOOS010000258         1616 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.266, whole genome shotgun sequence.
ACCESSION   DADOOS010000258 DADOOS010000000
VERSION     DADOOS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.266"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            34..1062
                     /locus_tag="J8C37_002872"
     CDS             34..1062
                     /locus_tag="J8C37_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAZ5306260.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1118..1336
                     /locus_tag="J8C37_002873"
     CDS             1118..1336
                     /locus_tag="J8C37_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306261.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          609 a          188 c          307 g          512 t
ORIGIN      
        1 aaatattata tttaaataag gagtatgata ataatgaata gcaaaaaact tcagatgggt
       61 gaaattgtta gaggtcctag tcaaatatct attgatgtta catataaatg taattttaga
      121 tgtaaacatt gttacaatgc gagtggtgga gacaattgtt ttatggataa tgagttgact
      181 gatgatgaac tgttagaact tacaaaagat atatgtaatt taaagccata taatatttgt
      241 ttctgtggag gagaaacttt actgagaaaa aaaatagttt tatcttgtgc cactttatta
      301 aaagaggcag gtattcctaa tattgctatt gttactaatg gatcattagt aactcaggat
      361 gttgcaaaag aattattcaa agcaggtatt aatcgatttc aagtaagttt agatggaaaa
      421 aatgctagta gccatgaagc acttagatta tatccaaaca gttttgaaaa ggcaattaat
      481 gctatcaaat tatttagaga attgtccgaa gaagtggatg ttgctttttg tcctactagt
      541 tttaattaca atcaattacg agaagtatat caactagtaa atactcttaa agtgaagtct
      601 ttaagaattc aaccatttat gcttagtggt aggggaatgc aaaataggga aatagtgcca
      661 actgtttctc aatatagaaa actagtttct gatattaact atattaaaag aaatgaagat
      721 tataccaaga tagattgggg agatccaatt gatcatatca tccgacacag aactttattg
      781 aaagattttt cgaattttgt ttctattagg tcagacggag gattaatggc atcaccttac
      841 ttgccaattc aagttggaaa tattaaatgt cacagtcttt ctaaatattg gcgagcagga
      901 ctttataaag ttttttcttt agatttagtt caagatatag ctactcaagt ttcttcaata
      961 caagatatga gtaaagaatt aaaaaaagga catattaaat ggcttaatga agatatagaa
     1021 attgatattc tcgatgaata caaacatgat attttaattt aggtttatca ttggaaagtt
     1081 tagataaaca aagtactttt agaaaggagg gaaaaaaatg atttctgaac ctcaaatgaa
     1141 taatatgtat gaaagcccaa ctataatggg agctggtgaa gctgcgccaa gagcttgttc
     1201 agcaattatg gtgtgtgtgt tattagcatc tgcaggagta ggtatccaag caggagcggt
     1261 gtatacttat gctgtagttg ctacagtagc tgtagctgca gccgcagttt ggaaaacagt
     1321 aggattagtt ggttaggaat taataggaga ctaactgtct cctattgatt ccaatgatta
     1381 agctaaggag gaattttaag aaaataatga aatacaataa tttaaaaccg aaattactta
     1441 atgagaacat agaatatata agagaagaaa attatggata tttaaccata gttcccaaaa
     1501 atggaactga attgaaagat aacgttttca atccaacagt aagatatata ttggctaact
     1561 gtactggaaa aaacagtgta aaaaaaatag cgaatggttt aaaaaagaag tttcct
//
LOCUS       DADOOS010000259         1611 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.267, whole genome shotgun sequence.
ACCESSION   DADOOS010000259 DADOOS010000000
VERSION     DADOOS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.267"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            233..1402
                     /locus_tag="J8C37_002874"
     CDS             233..1402
                     /locus_tag="J8C37_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ5306262.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          590 a          227 c          258 g          536 t
ORIGIN      
        1 tactatgttt gggttttata gcaaagaaag tttaataaga gtttggacct agcaaacaat
       61 attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc tacacagaca
      121 aaatgatagc gtaaaatatt ttgtgtaaat agatatttag ggtagaaaaa aagaaagtcc
      181 attctgtaaa attaaagtta ccacaaccga aattttatag gaggactttc tcatgcccaa
      241 ttttactaca gaaattatgg aaacactaat caataaaggt gatttagatg aattatttcg
      301 atgtcattta gaattagctg ttaattcatt attgcaggct gaattaacag cttttcttga
      361 ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat tcccgtaatg ggaattattc
      421 acgttcattc agaacagagt atggagaatt aaatttggta attcctagag atagaaatgg
      481 taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt tagaaaccac
      541 tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat tgattgaaaa
      601 aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa tagtttctga
      661 agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca tttttatgga
      721 tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt atattgtaat
      781 tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc caactgaatc
      841 tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa aagaggtttt
      901 attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg tttatccaaa
      961 tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata aggttcgtgt
     1021 tagtgatcga caagaaatct gtagcgattt caaattagtt tatcaagcaa gttcaaaaga
     1081 agaagcaaat aatcaaataa ggtttatgat agataaatgg aaaaagcagt atccacgagt
     1141 ggttaaattg ctcatgaatc ctgctatatt aaccttttat aatttccctc cgtcaatcag
     1201 aagaactatt tattcaacta atttgattga aggttttaac aaacaattaa agaaatatac
     1261 caaaagaaaa gagcaatttc ctaatgaaga atctctggag agatttctag tatctcaatt
     1321 caacaactat aatcaaaagt ttctatgccg agttcataaa ggttttaaag aaatacatga
     1381 tacattagaa tcaatgtttt aacttgatca acagaatggg cttttccatt tacacaaaat
     1441 tattgacgct cccgacaaaa ttatgatctt gcgtaaagtt ataaaatatt aataaaaata
     1501 ttttattaat gaaaagcttg gtagcacggt tattcatatg ctaatttatt tcactttata
     1561 gtaaggggag ttcttagtat attgttcaaa cttaaatttc atataattta a
//
LOCUS       DADOOS010000260         1588 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.268, whole genome shotgun sequence.
ACCESSION   DADOOS010000260 DADOOS010000000
VERSION     DADOOS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.268"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(264..563)
                     /locus_tag="J8C37_002875"
     CDS             complement(264..563)
                     /locus_tag="J8C37_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAZ5306263.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            914..1546
                     /locus_tag="J8C37_002876"
     CDS             914..1546
                     /locus_tag="J8C37_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAZ5306264.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          553 a          244 c          212 g          579 t
ORIGIN      
        1 tttttcaatc tatatttcca atttttaact tacaaaaatg caacattaat aaataacgat
       61 agtttcataa aatacacttt taattaacat tattagttta tactagctgt ttttgtgtta
      121 attaaaggat aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata
      181 cagactctgt tcaaataaat gaacctagcc tgcattatga gattgactat actttttaat
      241 tcattgagat gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt
      301 cctcgaccta attctttttc aaattttcct attgttgata tctccatata aaacggtgat
      361 actggtttag acaattctcc cctgattgct aacggacgta aactacgttc caaattattt
      421 atgactttag gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata
      481 cgttcctttt ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt
      541 tctatttctt cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac
      601 attctataaa caaagtatac gctacatcta gattttttta aatcattact ggattgttaa
      661 aacctttcaa tacactgtta tggcaacgat ttttatttta aaaacattct aaattaaatc
      721 agtaaaaatt tttattctaa gatttttatt agaattacgc ctaatttgtg tctctcaggg
      781 caattctaag aacgtttcaa gagatagcga cactatcgtt atcgaaaaag gcgaaaacct
      841 atctcatttt ctgctaatgt aagttggttt agcttttgtt aatatgactt tatgaaaatg
      901 gaggtaatta aatatgattt ttcctaaagt taatgaagca agcttgaata aaggatcctt
      961 ttttcatatc tatgaaaacg aagattttga actgtataat atcctttcac aaaaagaggt
     1021 catcgaaaac caaataatct gccatccaat tatttacaaa tctattttca agtatgcatt
     1081 ttttgcttcc aatgattttc aaagaatgga gattaccgac tgtatattcg aaaactgtga
     1141 tttttcaggc tgtatattga caaaaagcat gttgcatcgc gtgaaatttg tcaattgtaa
     1201 attaacaggt actaaattga tggaaagtta tattgggaac acgttattcg agaattgtaa
     1261 aatggactat gtaaatttat ctggttcaaa cataaaagaa agtacctttg agcactctgt
     1321 cttatcaagc gcagattttg tagactgttc cctcactaaa ataatgtttt ctactaatga
     1381 tttagaaaat gctagctttt ttgatactga tttaaaaaat atcgatttaa gtagcaactc
     1441 ctttgaaatg attgaagtac gctcggaaca tctaaaaaat tgtacgctta atcagttgca
     1501 agcacttggt tttgctagaa aatttttaca aataaaagta gtataatcaa aatgaatata
     1561 taagataaat ttttagcaat acaaatat
//
LOCUS       DADOOS010000261         1573 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.269, whole genome shotgun sequence.
ACCESSION   DADOOS010000261 DADOOS010000000
VERSION     DADOOS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.269"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DADOOS010000262         1539 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.270, whole genome shotgun sequence.
ACCESSION   DADOOS010000262 DADOOS010000000
VERSION     DADOOS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.270"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            54..734
                     /locus_tag="J8C37_002877"
                     /pseudo
     CDS             54..734
                     /locus_tag="J8C37_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            796..1413
                     /locus_tag="J8C37_002878"
     CDS             796..1413
                     /locus_tag="J8C37_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306265.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          561 a          231 c          301 g          446 t
ORIGIN      
        1 caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggacaga gtaatgaatc
       61 attttaaagg caaacaattc aaaaaagacg tcattattgt cgctgttggt tactacctgc
      121 gttacaatct aagctatcgt gaagttcagg aattgttata tgatcgtgga ataaatgttt
      181 gtcatactac gatttatcgt tgggtataag agtacagcaa agtcctctat tatctttgga
      241 agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat atcaaaatta
      301 agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc ttagatatct
      361 ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga ctccataaac
      421 agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc tccgccttta
      481 gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg aagtatctta
      541 acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt tatcaaagtc
      601 tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata tataaaaaga
      661 accgaagaaa tggaacgctc ttcggctttt cggtgtctac tgaaatcaag gtattaatgg
      721 gaataacagc ctaagatatt tggagttcag agagggcgcg tttgattttc aaacttcgca
      781 acagaaccga gaaccttggg ttttcaagtg ctttttggtt cattaatctt aaccatagtg
      841 tttttaaagt ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg aatagttctt
      901 attattttta ctgtgactgc agcaacagaa gcaaattatt cgcataaaat tcagtcgtat
      961 aaaggtcgtt tagttatgac caaaaaagat gttacacgtt atacatcctc aagaactgga
     1021 ggcctatatg actatgcacc attagatgca ttgaaatata aagatcattt aaagaaacat
     1081 gaagtaaaag ttggtgatcg gtgggagaat gcatctcata aagcatataa ttccaaaata
     1141 gtctatacag tagaagatgc cgatgagcaa aatatcacat tacctgtatt caattattgg
     1201 ggaacagttg tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa tggtttagtg
     1261 tctattaaag gtacggggaa aagaactata gttgaggttt catctacgta tccaactacg
     1321 atgattctat ctttattaat tagttcaata acttttatat tcttatttac acgttattta
     1381 ataagatgct gcggtgaaat gaaaatagat tagatagata ttataaatat gttgattcta
     1441 ttttcatagc aatggagaaa atacattagc aatatataaa agaaggtatc aagagtcaca
     1501 gtgaatttat tccgaaaagt gaaaagagca aatataaaa
//
LOCUS       DADOOS010000263         1528 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.271, whole genome shotgun sequence.
ACCESSION   DADOOS010000263 DADOOS010000000
VERSION     DADOOS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.271"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            15..1481
                     /locus_tag="J8C37_002879"
     CDS             15..1481
                     /locus_tag="J8C37_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAZ5306266.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          325 g          497 t
ORIGIN      
        1 ttggggtata gattatggaa aaacagcaag acaagggctt cttcgggcag ccgaagggat
       61 tgtcaacttt gttcttcact gagatgtggg aacgatttag ttattacggt atgcgtgcat
      121 tacttatcta ttatatgtac gatacagttg ccaacggggg acttggatta ccacgagcaa
      181 cagcattagc tatcatgtcg atctacggat ctttgatcta catgtctagt atcgtcggcg
      241 gatgggtatc tgaccgagtg ttaggagcaa aacgcactgt attcttcggt ggagttttca
      301 ttatgcttgg ccacatcgtc ttggctttac catttggtgt aagtacattg tttgtttcga
      361 tggcattgat tattatcgga accgcctttt tgaaaccaaa tgtttctggc atggtcggac
      421 atctttattc aaagactgac ttgcgaagag atgcaggatt ttcgattttt tacatgggtg
      481 tcaacttagg ggcattatta gcaccactaa tcgttggtac aatcggtcaa gaatataact
      541 atcatttagg gttctcttta gctgctattg ggatgttctt tggattatta caatacatca
      601 tccaaggacg caagacatta gacggagtgg gtacaacacc gccgaatcct ttgacaactg
      661 aagaacgtaa aggattcatc cgtaacttga tcatcgcact tgttgttatc gctgcagtat
      721 tcggcggggc acaactaact ggtcatctga caattgactt ttttgtcaat acaatcagtg
      781 tattggggat tatcttaccg ttgtattact ttatcaaaat gctgacttca aaagatgtaa
      841 ctgctgaaga aaaaccaaga gtatgggctt atatcccatt attccttgca gcaatcattt
      901 tctggtcatt acaagaacaa ggttcatcta tcttagcttt atttgctagt gaacgtacac
      961 aatctacgct atttggtgta acgatccaag caagctggta tcaatcattg aatccaattt
     1021 tcatctttat tctgacacca gtattcgtaa cgatgtggac gaaacttggt gaacgtcaac
     1081 catctacagt agtgaaattc tctcttggac ttttatttgc aggattatcg tacttgctat
     1141 tgatgttgcc gggtatgctt tatggtacaa gtggaaaagt aagtccattg tggttgttgg
     1201 gaagtttctt catcgtggtt attgctgaat tgtgtctttc accagttggt ttgtctgtga
     1261 caacaaaatt agcaccacga gcatttgaat cacaaacaat cgctatttgg ttcttagctg
     1321 atgcttcttc acaagcaatc aatgcacaaa ttgcacgctt ctacacaccg ggaacagaat
     1381 ctgcttactt cggtattatc ggtgcagtag caatcgtcgg tggtatcttg ctatttactg
     1441 tgaaagaacc aatcaaaaaa ctaatgggct ctattcatta atcaatgaac ataaaaacag
     1501 gaattgctta tttgcagcag ttcctgtt
//
LOCUS       DADOOS010000264         1497 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.272, whole genome shotgun sequence.
ACCESSION   DADOOS010000264 DADOOS010000000
VERSION     DADOOS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.272"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="J8C37_002880"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="J8C37_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAZ5306267.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          398 a          264 c          303 g          532 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaaca
//
LOCUS       DADOOS010000265         1492 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.273, whole genome shotgun sequence.
ACCESSION   DADOOS010000265 DADOOS010000000
VERSION     DADOOS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.273"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(717..>1492)
                     /gene="menA"
                     /locus_tag="J8C37_002881"
     CDS             complement(717..>1492)
                     /gene="menA"
                     /locus_tag="J8C37_002881"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAZ5306268.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          542 a          276 c          260 g          414 t
ORIGIN      
        1 tatgtaagat tgcttcgata attctcaaaa cgaaataagt agggaagttg gagattttga
       61 ttatcttcgt aactgacgaa gagtaaatga atccatttgg tatgcactct taagttcaaa
      121 atattggtat cgatgtttaa aagaaataga aatgtatcga agaacagatg tttaaaaaag
      181 tgtgactttg tataaataaa ttagaagaag caaacgagga tgttaaaaga ataccatata
      241 gttaccaaac caagaaagtt ctcgtcaagt tcgctggtac gaatcaaatc aagtgaaaaa
      301 agggactgtg aaaaaaattg tgtcatagtt agtcactttt tccgaacaaa tagtgttcaa
      361 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccatt
      421 tgtttctcta atactcagga caaaacactg attttacaac ctctgataaa cggtgttcaa
      481 aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc attttcacaa
      541 aatttttctt aatactcgaa actaagcgac tttttcacaa cctcctgata aacggtgttc
      601 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga gctattttta
      661 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
      721 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      781 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      841 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      901 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      961 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
     1021 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
     1081 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
     1141 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
     1201 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
     1261 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
     1321 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
     1381 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
     1441 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta at
//
LOCUS       DADOOS010000266         1460 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.274, whole genome shotgun sequence.
ACCESSION   DADOOS010000266 DADOOS010000000
VERSION     DADOOS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.274"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(34..462)
                     /locus_tag="J8C37_002882"
     CDS             complement(34..462)
                     /locus_tag="J8C37_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAZ5306269.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            554..1414
                     /locus_tag="J8C37_002883"
     CDS             554..1414
                     /locus_tag="J8C37_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAZ5306270.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          470 a          278 c          245 g          467 t
ORIGIN      
        1 tttaaattat caaatgttat tttatttaaa tccttattga caattttcac atcttccgaa
       61 aatctcgaat ctatgccctt cgatcgtgca tccacctagc tgttcttcga aataattcat
      121 tggacacata tggatttctt tcgttgttcc gcacactgta cagataaaat gatggtgatg
      181 gccgatttcc ccctgacagc aatgaaaacg aaacttcatt tcaccattta aatcggtttc
      241 ttccagtaaa tcgatctctg caaaatcatg gagattccgg taaatcgtat cataacttac
      301 ccctttgaat tcctgattca tatagtcaaa aacttcttta gcagatacgt agcggttacg
      361 cttcactaaa taagttaaca atgcttctct ttttttagta tatttaaaac cgttattttt
      421 caatgtttct aatgattgtt cgatcaatgt ctgttttttc atgacttatt cctccaaatc
      481 aaaatgatta cgaagtacgc attctagagt atggtataat aaacataaaa aaaatgcaag
      541 tgaggtttta tccatgtctt ccgattctac agaaatcgtt actttttttg tcatctccga
      601 ctctgctgga gaaactgcga caaaactagc gcaagctact atggctcagt atcctaccgt
      661 tgagttcaac ctattccgca gaacgttcgt gacagatgaa aaaacattaa ggcaggcatt
      721 gcaagatgca ttagaagaaa aagcacttgt cctgcataca ttaatcaatc aagaactgat
      781 cgagataaca cgttcttttt gtgaagaaca tcaattattt agtttcgatg tgatgacgcc
      841 accgatatca gaaattgaac gtttaactgg tatcaagcca attcggcaac ctggtgccct
      901 ccatttattg aatgagaact atttcaaacg gatcaaagca atggaatttg ctgtaaaata
      961 cgatgatggg aaagatcctc gtggtttttt agaagcagat gtcgttttgc taggcgtatc
     1021 ccgaacttcc aaaacgcctt tgagcttgtt tttagcaaat aaaaatttaa aagttgctaa
     1081 tttacctttg attccccaag cacatattcc caaacagctg tgggaaatcg atcctaaaaa
     1141 aatcgtggga ctaacgaata acccagatat tttaaataat atccgtaaag aacggatgcg
     1201 ctcttacgga ttaaaccctg atactgctta ttccgatatc gaaaaaatta gagctgaact
     1261 cgattttgcg aatgatcttt atgaaaaact aggatgtgta gtgatcaatg tggcatcttt
     1321 atctattgaa gaaacggctt cgcttatttt gaatgcttta gacctagaag atcatagtta
     1381 ttatggaact gaaacaacag aaaaaagcga ttaatcattt ttgtcttttt cttaaaaaac
     1441 actggagtgt gacaacgaaa
//
LOCUS       DADOOS010000267         1440 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.275, whole genome shotgun sequence.
ACCESSION   DADOOS010000267 DADOOS010000000
VERSION     DADOOS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.275"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            105..1406
                     /locus_tag="J8C37_002884"
     CDS             105..1406
                     /locus_tag="J8C37_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAZ5306271.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DADOOS010000268         1428 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.276, whole genome shotgun sequence.
ACCESSION   DADOOS010000268 DADOOS010000000
VERSION     DADOOS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.276"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..319
                     /locus_tag="J8C37_002885"
     CDS             <1..319
                     /locus_tag="J8C37_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAZ5306272.1"
                     /translation="RRNPYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFK
                     PEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYL
                     YRR"
     gene            324..1271
                     /locus_tag="J8C37_002886"
     CDS             324..1271
                     /locus_tag="J8C37_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAZ5306273.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          486 a          199 c          294 g          449 t
ORIGIN      
        1 aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta ctatagttaa
       61 taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga atgcctcaat
      121 gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg
      181 gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag attttaaatg
      241 gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag atatctataa
      301 ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg gctggcttca
      361 ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc gtcgatgact
      421 tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt attgagggag
      481 atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt gattatatct
      541 ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta gaaacacatc
      601 gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat caacctgatt
      661 taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa
      721 aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa ttcgctgcag
      781 aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca gttcgttttt
      841 tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg atttctattc
      901 tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca ttatttggtg
      961 atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca ttacttttag
     1021 ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt aaagcaacca
     1081 cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg acacttgaat
     1141 acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact aagatccagt
     1201 caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat ttgaaaactg
     1261 aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct ttgtcaataa
     1321 ggactgatga gttttgtaaa atcaaatctg ggatagaagg gctctttgtc aaatgatgtt
     1381 ggtaaagaga aatcttagaa taatttggac agaacagaaa gaagaaaa
//
LOCUS       DADOOS010000269         1418 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.277, whole genome shotgun sequence.
ACCESSION   DADOOS010000269 DADOOS010000000
VERSION     DADOOS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.277"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(150..1337)
                     /locus_tag="J8C37_002887"
     CDS             complement(150..1337)
                     /locus_tag="J8C37_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAZ5306274.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DADOOS010000270         1416 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.278, whole genome shotgun sequence.
ACCESSION   DADOOS010000270 DADOOS010000000
VERSION     DADOOS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.278"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(128..418)
                     /locus_tag="J8C37_002888"
     CDS             complement(128..418)
                     /locus_tag="J8C37_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306275.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(536..796)
                     /locus_tag="J8C37_002889"
     CDS             complement(536..796)
                     /locus_tag="J8C37_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306276.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(933..1346)
                     /locus_tag="J8C37_002890"
     CDS             complement(933..1346)
                     /locus_tag="J8C37_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAZ5306277.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          523 a          235 c          208 g          450 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaa
//
LOCUS       DADOOS010000271         1412 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.279, whole genome shotgun sequence.
ACCESSION   DADOOS010000271 DADOOS010000000
VERSION     DADOOS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.279"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..625)
                     /locus_tag="J8C37_002891"
     CDS             complement(<1..625)
                     /locus_tag="J8C37_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAZ5306278.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="J8C37_002892"
     CDS             complement(784..1050)
                     /locus_tag="J8C37_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAZ5306279.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="J8C37_002893"
     CDS             complement(1040..1396)
                     /locus_tag="J8C37_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAZ5306280.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          388 a          277 c          178 g          569 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca ta
//
LOCUS       DADOOS010000272         1410 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.280, whole genome shotgun sequence.
ACCESSION   DADOOS010000272 DADOOS010000000
VERSION     DADOOS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.280"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            90..311
                     /locus_tag="J8C37_002894"
     CDS             90..311
                     /locus_tag="J8C37_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306281.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(635..880)
                     /locus_tag="J8C37_002895"
     CDS             complement(635..880)
                     /locus_tag="J8C37_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAZ5306282.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(874..1275)
                     /locus_tag="J8C37_002896"
     CDS             complement(874..1275)
                     /locus_tag="J8C37_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAZ5306283.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          479 a          229 c          200 g          502 t
ORIGIN      
        1 tttgttttca aaagagactg tagtacccct ttttttcact aaaagcccac tgtagtcatt
       61 gatatttttg ataagaaagg aacataatta tgcgtaatga aattgctaat ataacaataa
      121 tccgagaaac tttacaaaac catactgcaa atgagatctc taaacataca ggattaaatt
      181 tgagtactat aaaaaaacta aaaagtggag aacgttcaat agaaaaacta aatttacatg
      241 atgctatttg cctcactgaa tttggtctta aaaataatag aaaaaatgtt gaaatcaaca
      301 tatggaaata attcttaatc agctgctttt gtcaaaagca cataggaatt tcacctctct
      361 ccaagtttac ggcgagcctt acggaagtat cattatccca acttcaaatc atcgcttttt
      421 attgactata aaaattattt tagaatgttt atcgctcctt ctttacagta agaaatcatg
      481 gcgcaactaa aatatgctcc ttgaagtgtt aacgtactga tagaatgtat ttaaatttca
      541 atgatcaata ggttttctgt ttaaaagaat ctactaatcc tttaactatt agaagattct
      601 tttaaacaga ataagtggaa aggggacttt ttagttagtt aattttaaat tttaagatcg
      661 cttctatcaa tttaatttct agctgtctta gcatatactc atctacatag gtaataaatt
      721 gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta tgataatgtt
      781 tgagtatcat cttgattgaa attggatctt cttctagagc agattggata atggatagag
      841 gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc ccatttttaa
      901 ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat tgaggatttt
      961 accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct ttcttcttat
     1021 ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata tttccattcc
     1081 aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac cttcatttga
     1141 attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa tagacttagt
     1201 ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag atttctcatt
     1261 gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat acaacaatca
     1321 gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac agaaaatctt
     1381 tttataaaaa aaaggagttg gcaattaagc
//
LOCUS       DADOOS010000273         1384 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.281, whole genome shotgun sequence.
ACCESSION   DADOOS010000273 DADOOS010000000
VERSION     DADOOS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.281"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            38..>1384
                     /locus_tag="J8C37_002897"
     rRNA            38..>1384
                     /locus_tag="J8C37_002897"
                     /product="23S ribosomal RNA"
BASE COUNT          422 a          270 c          406 g          286 t
ORIGIN      
        1 tctatcgcta gaagaaggat caaaacccaa ccgtaaggtt gataaggtta agtgaataag
       61 ggcgcacggt ggatgccttg gcactaggag ccgatgaagg acgggactaa caccgatatg
      121 ctttggggag ctgtacgtaa gctatgatcc agagatttcc gaatggggga acccaacatc
      181 tttaatagga tgttacgatt gtgtgaatac atagcacatt cgaggtagac gcagagaact
      241 gaaacatcta agtacctgca ggaagagaaa gaaaattcga ttccctgagt agcggcgagc
      301 gaaacgggaa aagcccaaac cagcaagctt gcttgttggg gttgtaggac tccaatatgg
      361 tagttctttc agatagtcga atgacttgga aaagtcagtc aaagagggta aaaaccccgt
      421 agacgaaatg tggaagacac ctaggaggat cctgagtacg gcggaacacg agaaattccg
      481 tcggaatccg ggaggaccat ctcccaaggc taaatactcc ctagtgaccg atagtgaacc
      541 agtaccgtga gggaaaggtg aaaagcaccc cggaagggga gtgaaataga acctgaaacc
      601 gtgtgcctac aacaagtcaa agcccgttaa tgggtgatgg cgtgcctttt gtagaatgaa
      661 ccggcgagtt acgattgcat gcgaggttaa gttgaagaga cggagccgca gcgaaagcga
      721 gtctgaatag ggcgtttgag tatgtagtcg tagacccgaa accatgtgat ctacccatgt
      781 ccaggttgaa ggtgcggtaa aacgcactgg aggaccgaac ccacgtacgt tgaaaagtgc
      841 ggggatgagg tgtgggtagc ggagaaattc caaacgaact tggagatagc tggttctctc
      901 cgaaatagct ttagggctag cctcggaatt gagaatgatg gaggtagagc actgtttgga
      961 ctaggggccc atctcgggtt accgaattca gataaactcc gaatgccatt cattcatatc
     1021 cgggagtcag actgtgagtg ataagatcca tagtcgaaag ggaaacagcc cagaccacca
     1081 gctaaggtcc caaaatatat gttaagtgga aaaggatgtg gggttgcaca gacaactagg
     1141 atgttggctt agaagcagcc accatttaaa gagtgcgtaa tagctcacta gtcgagtgac
     1201 cctgcgccga aaatgtaccg gggctaaaca tattaccgaa gctgtggagt acacctttag
     1261 gtgtattggt aggagagcgt tctaagggcg ttgaaggcag atcgtgagga ctgctggagc
     1321 gcttagaagt gagaatgccg gtatgagtag cgaaagacag gtgagaatcc tgtccaccga
     1381 atga
//
LOCUS       DADOOS010000274         1374 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.282, whole genome shotgun sequence.
ACCESSION   DADOOS010000274 DADOOS010000000
VERSION     DADOOS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.282"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            33..1372
                     /locus_tag="J8C37_002898"
     CDS             join(33..474,474..1372)
                     /locus_tag="J8C37_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAZ5306284.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DADOOS010000275         1373 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.283, whole genome shotgun sequence.
ACCESSION   DADOOS010000275 DADOOS010000000
VERSION     DADOOS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.283"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            191..445
                     /locus_tag="J8C37_002899"
     CDS             191..445
                     /locus_tag="J8C37_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAZ5306285.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="J8C37_002900"
     CDS             446..763
                     /locus_tag="J8C37_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAZ5306286.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DADOOS010000276         1364 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.284, whole genome shotgun sequence.
ACCESSION   DADOOS010000276 DADOOS010000000
VERSION     DADOOS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.284"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="J8C37_002901"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="J8C37_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAZ5306287.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKFHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          394 a          241 c          215 g          514 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaaacttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaattg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DADOOS010000277         1352 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.285, whole genome shotgun sequence.
ACCESSION   DADOOS010000277 DADOOS010000000
VERSION     DADOOS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.285"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="J8C37_002902"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="J8C37_002902"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAZ5306288.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..>1352
                     /locus_tag="J8C37_002903"
     CDS             1131..>1352
                     /locus_tag="J8C37_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306289.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKL"
BASE COUNT          413 a          273 c          205 g          461 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tg
//
LOCUS       DADOOS010000278         1336 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.287, whole genome shotgun sequence.
ACCESSION   DADOOS010000278 DADOOS010000000
VERSION     DADOOS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.287"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            366..1277
                     /locus_tag="J8C37_002904"
     CDS             366..1277
                     /locus_tag="J8C37_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAZ5306290.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          537 a          200 c          256 g          343 t
ORIGIN      
        1 gtataaaaat atatcataat aaaaacaaca aaaaaatgtc tggaaaatcg aacataaaag
       61 tgtctgattt gaatatccca gcagcggaag aagaaaaaca aaatcgataa catttgttct
      121 aagaattagt tacgttaaat acggaacgca agatagactt gaaaaacggt aacaaaagaa
      181 gtagtatatt aaggtgtgat gtgctacttg acatttattc tgctgagcga actttctgga
      241 ttgtgtagcc ttatatctat tcaggacgta tcgagcagat tagtaaaggt gttcttgatg
      301 attttcatct tgaacacctt tgtttttagc aattcgcaaa aaacgaaaag taggagaatt
      361 gataaatgaa aaaaagattt ttagcacttg ccatcgtact aggcactggc ttattgtcag
      421 gctgtacgaa tgccggtgaa aaaacagccg ttagttataa aggcggtaca atcagcgaac
      481 aagaagtaat ggatagcttg aaaaaaatgc aaggagcaga ttccgctgtg caacagttga
      541 tcgtttatca agtatttgaa gataaatatg gagatgacgt atcaaccaaa gaaatcgatt
      601 ctcaatatga tcaaacaaag aaacaattgg gtgattcatt tgattcacaa ttgaaatctg
      661 caggttacac agaacaaaca ttcaaagaca gtatcaaaca aagtctagcc tttcaagaag
      721 gtctaaagaa acatatcaaa ttgaccgatg aagatttaaa aacggcttgg gaatctttcc
      781 atccagaagt ggaagctcaa attatccaag tagcaagtga agatgatgca aaagatgtga
      841 agaaagctgc tgataaggga gacgatttct ctaaattagc caaagacaag tcaactgata
      901 ctacgacaaa agaagatggc ggaaaagtga aatttgattc cactactaca acggttccgg
      961 cagaagtcaa agaagcagcc tttaagttga aagatggtca agtttctgat gtgatcactt
     1021 ctaccaatgc ttcaacttat actacggaat attatgtggt taaaatggtg aaaaaccaaa
     1081 acaaaggcaa cgacatggat aaatacaaaa aagaattgaa agaaattgca acagacacaa
     1141 aattaagtga cagtaccttc cagaataaag taattggcga agtattgaaa gacgctaatg
     1201 taaaaatcaa agacaaagac tttgaaaatg ttttgagtac attcacttca gattctagta
     1261 cagcaagtag taaataagcg gtataaataa aaaatatgaa ctatttaaat caataaaact
     1321 gttcatctaa atcttt
//
LOCUS       DADOOS010000279         1333 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.288, whole genome shotgun sequence.
ACCESSION   DADOOS010000279 DADOOS010000000
VERSION     DADOOS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.288"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(305..919)
                     /locus_tag="J8C37_002905"
     CDS             complement(305..919)
                     /locus_tag="J8C37_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAZ5306291.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          432 a          264 c          222 g          415 t
ORIGIN      
        1 caacgtgtat taagagacaa gaaagaatcc atatggattc tttcttgtct ctttttccac
       61 tactttttat ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca
      121 acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta
      181 aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt
      241 ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg
      301 ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct
      361 gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca
      421 aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa
      481 ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa
      541 caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt
      601 ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa
      661 taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca
      721 atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg
      781 tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc
      841 cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt
      901 caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca
      961 gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct
     1021 ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa
     1081 ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat
     1141 taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt
     1201 gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt
     1261 ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca
     1321 agaaaataca aaa
//
LOCUS       DADOOS010000280         1333 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.289, whole genome shotgun sequence.
ACCESSION   DADOOS010000280 DADOOS010000000
VERSION     DADOOS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.289"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            83..>1333
                     /locus_tag="J8C37_002906"
     CDS             83..>1333
                     /locus_tag="J8C37_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAZ5306292.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          419 a          262 c          255 g          397 t
ORIGIN      
        1 ttttttgtat aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta
       61 ccaattcata ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat
      121 tctagaccta aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca
      181 aatttgcttg atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct
      241 ttgtgtcgtc ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa
      301 tgatgtgtct gagtatcgca cttatctgga gctgaagaaa caacgattta aatgtaagtc
      361 ttgccaaaga acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca
      421 aaaagttcgt tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc
      481 tcgtcagaaa aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc
      541 attgaatcct ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt
      601 tcgaagtgtt gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt
      661 ggatattgtc gaaaatcgac gcttgcctta tttagaacgc tatttttctc ggttttcatt
      721 agcgactcgt gaagccgttc aattgattgt tattgatatg tatgcgcctt atgtttcgtt
      781 ggtgaaaaag cttttcccga atgctcagtt gatcatcgat cgtttccaca tcgtccaaca
      841 tattggcaga actttcttga atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa
      901 ttcccactct caacgagggt tagggaaaaa attgaagcgc tattggaaac tcctacaaaa
      961 agaagaagca aagttaaact atgaaaaacg ggtttggcgt gccagtttta aagattattt
     1021 gactgaaagt gagattgccg atcgactgtt agctgcttgt cccaacttac gccaaggcta
     1081 tcagttatac caagacattc tttatgcggt aaagaaaaga gatcagtcac tatttgagga
     1141 ttgtttaact agagacatta gcggtctgcc agaaacctat acaaccactt tacgaacgtt
     1201 taaaaagttt ctgccacaga tccaaaatgc gcttcactac agctattcaa acggaccatt
     1261 ggaatgctta aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct ttcgcaactt
     1321 ttacaatttc aaa
//
LOCUS       DADOOS010000281         1329 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.290, whole genome shotgun sequence.
ACCESSION   DADOOS010000281 DADOOS010000000
VERSION     DADOOS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.290"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..848
                     /locus_tag="J8C37_002907"
     CDS             <1..848
                     /locus_tag="J8C37_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ5306293.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          431 a          243 c          258 g          397 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaattcggtt
     1321 ctgttgcaa
//
LOCUS       DADOOS010000282         1324 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.291, whole genome shotgun sequence.
ACCESSION   DADOOS010000282 DADOOS010000000
VERSION     DADOOS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.291"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            278..820
                     /locus_tag="J8C37_002908"
     CDS             278..820
                     /locus_tag="J8C37_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306294.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            910..>1324
                     /locus_tag="J8C37_002909"
     CDS             910..>1324
                     /locus_tag="J8C37_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAZ5306295.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DADOOS010000283         1320 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.292, whole genome shotgun sequence.
ACCESSION   DADOOS010000283 DADOOS010000000
VERSION     DADOOS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.292"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>1320
                     /locus_tag="J8C37_002910"
     rRNA            <1..>1320
                     /locus_tag="J8C37_002910"
                     /product="23S ribosomal RNA"
BASE COUNT          349 a          291 c          403 g          277 t
ORIGIN      
        1 gttcctgatg tcacactgcc gagaaaagct tctagtgaga aaacagcggc ccgtaccgca
       61 aaccgacaca ggtagtcgag gagagaatcc taaggtgagc gagagaactc tcgttaagga
      121 actcggcaaa atgaccccgt aacttcggga gaaggggtgc tgatcatacg atcagccgca
      181 gtgaataggc ccaagcgact gtttatcaaa aacacaggtc tctgcaaaat cgtaagatga
      241 agtatagggg ctgacgcctg cccggtgctg gaaggttaag aggagtgctt agcgcaagcg
      301 aaggtacgaa ttgaagcccc agtaaacggc ggccgtaact ataacggtcc taaggtagcg
      361 aaattccttg tcgggtaagt tccgacccgc acgaaaggcg taacgatttg ggcactgtct
      421 caacgagaga ctcggtgaaa ttttagtacc tgtgaagatg caggttaccc gcgacaggac
      481 ggaaagaccc catggagctt tactgtagtt tgatattgag tgtctgtacc gcatgtacag
      541 gataggtagg agccgtagaa atcggaacgc tagtttcgat ggaggcgctg gtgggatact
      601 acccctgcgt tatggccact ctaacccgca ccactaatcg tggtgggaga cagtgtcaga
      661 tgggcagttt gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc
      721 tcagaatggt tggaaatcat tcgaagagtg taaaggcaga agggagcttg actgcgagac
      781 caacaagtcg agcagggacg aaagtcgggc ttagtgatcc ggtggttccg catggaaggg
      841 ccatcgctca acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagtcc
      901 acatcgacgg ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg
      961 gtcccaaggg ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg
     1021 tgagacagtt cggtccctat ccgtcgcggg cgttggaaat ttgagaggag ctgtccttag
     1081 tacgagagga ccgggatgga cttaccgctg gtgtaccagt tgttctgcca agggcattgc
     1141 tgggtagcta tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa gcccacctca
     1201 agatgagatt tcccatttct ttaagaaagt aagatccctg agagatgatc aggtagatag
     1261 gtcaggagtg gaagtacagt gatgtatgga gcggactgat actaatcgat cgaggactta
//
LOCUS       DADOOS010000284         1261 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.293, whole genome shotgun sequence.
ACCESSION   DADOOS010000284 DADOOS010000000
VERSION     DADOOS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.293"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            150..1190
                     /locus_tag="J8C37_002911"
     CDS             150..1190
                     /locus_tag="J8C37_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAZ5306296.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          507 a          178 c          206 g          370 t
ORIGIN      
        1 agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa cagttttaat
       61 gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat tttacataaa
      121 taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt aaggcggata
      181 gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct gacgaataca
      241 aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga ttagagatcg
      301 caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat acgacaaatg
      361 aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa aaagagttgg
      421 aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta gggaaaaata
      481 atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc tacttactcg
      541 aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg aaaatcatac
      601 ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt atgaaaatca
      661 taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa cttaataata
      721 atatattaga cactaataga cacaatatag acactgaaaa agaccgccta caagatcaat
      781 tgttgttaga caattttgag acaattatga caaacgacag cattgctacg tttgtccctg
      841 aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct caaaaaaccg
      901 tccagactat tcataatgca aagaaaaaag ctgaaataga aagtggtatt tcgatagttt
      961 ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg acactgttaa
     1021 aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg atttttgtct
     1081 atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt gaaaaacgtt
     1141 tgaattatga aagctcccca agcactatta cgaaagactg gttagagtga aactttactg
     1201 acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aaatttttta ttattctaat
     1261 t
//
LOCUS       DADOOS010000285         1251 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.294, whole genome shotgun sequence.
ACCESSION   DADOOS010000285 DADOOS010000000
VERSION     DADOOS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.294"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            6..1085
                     /locus_tag="J8C37_002912"
     CDS             6..1085
                     /locus_tag="J8C37_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5306297.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          369 a          300 c          306 g          276 t
ORIGIN      
        1 ttcacatgac gcaaacccat gataacacag tttcacgcaa gggaaaacat ctttcctact
       61 cagaacgttg ccaaatcgct atcctgaaaa aggaaggcta ttccaaccgt caggttgcca
      121 aagtgctggg acgtgttccg caaaccataa acaatgagat tgatcgcgga accatcaccc
      181 agttaaaacg ccaaacgcaa aatggaaaag tgtatgacac ctactattct gtttatgacg
      241 ctgatgcagg acaggccttc tatgaaaagc aacgactgaa ctgtggcaga cgccccaaat
      301 gggcgaacac aaatgccttc attgattggg cagatgacaa aatgttaaat gaaaaatggt
      361 cgccggacgt cgtggtcgga tttgccagaa aacacgagct gttcgattgt gccatcattc
      421 ctggcacaac cgctctttat caatggattg accgcggaat catgcgaaca aagaacatgg
      481 acttgcttga aaaactgtcc cgtaaaatca aagcggtccg cccaggacac cgtcccaaca
      541 agcgtgttct cgggacgtca attgaagaac gccctaaaga agttgaaaca cgggaaacgt
      601 tcggtcattg ggaaatcgac acggtagtgg gaaacaagac caagtcggat gccgtgctgc
      661 tgacgctggc tgaacggcag acacgctttg aagtcatcct aaagttgaac gggaaggaca
      721 ccgaatcggt ggaccaagcg gttcaacagc taagagaacg ggctggcgct aacttcgatc
      781 aattatttaa gaccgttaca tccgacaatg gctcggagtt ttctgggcta cacgatgctc
      841 tgcaagatgt agctgacgtc tatttcagcc acccctatgc ttcttgggag cgtgggacga
      901 gcgaaaacca gcacaaatta attcgccgtt tcgtccccaa gggaagcccg attagtgagg
      961 tgagcgagaa ccagattttg cggatccagc gttggatgaa cgattacccg cggaaaattt
     1021 tgggttatgc gacaccacat gatacattcg tccaggcatt caagcaagaa cggttggtgg
     1081 cttgacccat tattccttga taacgatcct cactcgggac atggcttcat gtctggaaag
     1141 ctggcctggg gccagaatcg tctgaagact atcatacctc agttcattat caaggcctca
     1201 ctgatttaga ataacgaggc agttacggta tcaaacaaat gagttacctg t
//
LOCUS       DADOOS010000286         1245 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.295, whole genome shotgun sequence.
ACCESSION   DADOOS010000286 DADOOS010000000
VERSION     DADOOS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.295"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1244
                     /locus_tag="J8C37_002913"
     CDS             <1..1244
                     /locus_tag="J8C37_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAZ5306298.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DADOOS010000287         1238 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.296, whole genome shotgun sequence.
ACCESSION   DADOOS010000287 DADOOS010000000
VERSION     DADOOS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.296"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            36..>1238
                     /locus_tag="J8C37_002914"
     CDS             36..>1238
                     /locus_tag="J8C37_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAZ5306299.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHI"
BASE COUNT          412 a          240 c          242 g          344 t
ORIGIN      
        1 cgacaaaata aacaactgag aggacgatat gctatatgaa tgattctatc aaaaaaatgc
       61 tgcgattaat agagaaagat ttgatgatta cagaggtctc ttacgagacc tttcagaaga
      121 aaaagacgtt gatcgtcgat gctgttttct cacctgctcc ccatacctgt agaaattgtg
      181 gttctactgt ggtagatgga aacgggaaag taattgtagt gaaaaatggg aagaaggaaa
      241 cgattgtccg ctttgaacaa tacaatcaca tgcctttggt tatgcgctta aaaaagcagc
      301 gctatacctg taaaaactgt cgaacccatt ggacggctca aagttatttt gttcaaccca
      361 gacattcaat cgcgaatcat gtcagatata aaattgcttc tttactgact gaaaaagtat
      421 ctttatcttt tattgcgaaa aattgtcagg tatctttgac caccgttatt cgtacgttga
      481 aagagtttaa aagctactta ccaaagcaat ctaagaggat tctccccaga gtattgatgg
      541 ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt tgcgcagatg
      601 gcgaaacagg acaattaatt gatgtcttgc ctacgcgtaa attgcctcga ttaacaagct
      661 atttcttagg ctgcgctaat cctgaagaag taaaattttt agtcacagac atgaacgccg
      721 cctacttcca gcttaccaaa cgtattctgc caaatgcgaa agtagtgatt gatcggtttc
      781 atattatcaa acacatgaat caagccttta atgagttgcg tatccgtgaa atgaatgaac
      841 ttcgtaaagt aggacagaaa agtcaggcag aaaaactgaa aaagaactgg cgctttttgc
      901 taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt tttcgagcac
      961 caaaataccc atttcttacg gaagcgatga tgattgatcg attgcttggc ttttctacgt
     1021 ccctgaagga ggcgtatccc tattttcatg aattagttga agcctttcga gacaaagacc
     1081 ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac ggctttcggg
     1141 aaaagctcca aaaccttctg acctatgaag aaggcatcac caacgcgatg atctatcctt
     1201 acaccaatgg aaaaatagaa gcgaagaata cccatata
//
LOCUS       DADOOS010000288         1230 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.297, whole genome shotgun sequence.
ACCESSION   DADOOS010000288 DADOOS010000000
VERSION     DADOOS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.297"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(61..279)
                     /locus_tag="J8C37_002915"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="J8C37_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="J8C37_002916"
     CDS             complement(545..739)
                     /locus_tag="J8C37_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306300.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          435 a          207 c          168 g          420 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac
//
LOCUS       DADOOS010000289         1206 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.298, whole genome shotgun sequence.
ACCESSION   DADOOS010000289 DADOOS010000000
VERSION     DADOOS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.298"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            414..946
                     /locus_tag="J8C37_002917"
                     /pseudo
     CDS             414..946
                     /locus_tag="J8C37_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
BASE COUNT          387 a          223 c          224 g          372 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaattcggg acagatccat tgaaaaaact tgtgactata aagtaaaaga atacttatga
      961 aacacacaaa gaggtcgtga gcctgccgtt tcactccgag tcacaaggag caatttcaaa
     1021 aaatagcttc tcatgtttat taaatattgt gactggtgac cgaggagctg gcaggagaac
     1081 accgtttatt ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
     1141 caaaaatagc ttctcatatt tttcatttgg ttggacttat taccgcagat gcaagctttt
     1201 ggacac
//
LOCUS       DADOOS010000290         1201 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.299, whole genome shotgun sequence.
ACCESSION   DADOOS010000290 DADOOS010000000
VERSION     DADOOS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.299"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1094
                     /locus_tag="J8C37_002918"
     CDS             join(<1..208,208..1094)
                     /locus_tag="J8C37_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAZ5306301.1"
                     /translation="GKPRVEIIREYELTASAFDKWVKQSKTSGSFKEKDNLTPEQKEL
                     LELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHLYPISAMCRILGLSRQSYYYQS
                     KPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSSY
                     TVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFN
                     REILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINR
                     SLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLG
                     YETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          428 a          215 c          242 g          316 t
ORIGIN      
        1 ggaaagccac gtgtagaaat cattcgagaa tatgaactaa cagcttcagc atttgacaaa
       61 tgggtaaagc aatctaaaac gagtggttca ttcaaagaaa aagataatct tacgcctgaa
      121 caaaaagaat tgttagaact acgtaaaaga aaccagcaat tagaaatgga aaatgatatt
      181 ttaaagcaag cagcgctgat attcggacga agagacaagt aatcgatgcg aataagcatc
      241 tttaccctat atcagcgatg tgcagaatat taggtctatc acgtcagtcc tattattatc
      301 aatcaaaacc aaagaaagac gaatcagaac ttgaagaagt agtcgctgaa gaatttatcc
      361 gcagccgaaa ggcctacggc tcaagaaaaa taaaaaaagc cttatcaaaa cgaggcattc
      421 agatcagccg acgaaaaatt agtagaatca tgaaaaatag aggattaaaa tcgagctata
      481 ctgttgctta ttttaaagta catcattcta cttgcaatga agccaaaacg acaaacgtat
      541 tgaatcgtaa attcttaaga gacaacccat tagaagcgat cgtaacagac ttgacttatg
      601 tacgagtcgg gaaaaaatgg aattatgtct gtttcatttt ggatctgttc aatcgagaaa
      661 ttctcggcta ttcttgtgga gaacataaag atgccgttct agtaaaaaaa gcatttagcc
      721 gtatcaaaca acctctgaca gaggttgaga tttttcatac tgatcgtgga aaagagtttg
      781 ataaccaagc tattgatgaa ttattaacaa cttttgacat caatcgatca ttgagtcata
      841 aaggctgtcc ttttgataat gccgtagctg aatcaactta taagtcgttg aaagtagaat
      901 ttgtctatca atacacattt gaaaccttac aacaattgga tttggagtta tttgactatg
      961 tcaattggtg gaaccacctt cggttgcacg gtacacttgg ctacgagaca ccggttggtt
     1021 accgtaacca gagattggcg cagcgaatcc ttgataatga gctcggatgt gctaacgcta
     1081 gcgaggcagt ctaactttaa ctgttagctc ctgccgaaga tcgtcacatc cgaggaggct
     1141 cattgtcaag gacaatcgga atagcatacg gaaagaagtt ctgcacctta taaaatttgt
     1201 c
//
LOCUS       DADOOS010000291         1193 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.303, whole genome shotgun sequence.
ACCESSION   DADOOS010000291 DADOOS010000000
VERSION     DADOOS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.303"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..1076
                     /locus_tag="J8C37_002919"
     CDS             <1..1076
                     /locus_tag="J8C37_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAZ5306302.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DADOOS010000292         1166 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.304, whole genome shotgun sequence.
ACCESSION   DADOOS010000292 DADOOS010000000
VERSION     DADOOS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.304"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            143..928
                     /locus_tag="J8C37_002920"
     CDS             143..928
                     /locus_tag="J8C37_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAZ5306303.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          451 a          181 c          182 g          352 t
ORIGIN      
        1 atttaattct gcacccaaaa agatacactc ctatctctta tttaaagttg ctatataccc
       61 tcttatttta gcatactccc ttttagaaaa agcaaaaaaa aaagaaaaaa tcttattctt
      121 gataacaagc tacgtgacgt atatgcctaa caaaaataga acttttgaat caatcttaaa
      181 tgaaggttta accacagaac cggcatccat tatttttgat gaagttatcg atgacgttga
      241 agatagtaag tatcataaaa atttaaaaga acttttttat tttgcactca acaatggacc
      301 aagtttgttc actggtatat cagtacctga tagtgataat ccattaatta tggcaaaaat
      361 ttacttaaca aaatggtgta ataaatacat cagggatagg aataacccag cattaaaaaa
      421 acctcttaaa acatttggcg aaaaagatgc tgctttaact actagagtag cagcaaatgc
      481 taatattgat gatcaaaaag tattgaatga ctacttgcgt ggacattttt tatatatgag
      541 cgctgagaac atgaacggaa gtattttaga ggaatattta gccgaagtgt tagaacctga
      601 aggttggatt tggtgcgccg gctctgtata tcgagctgtt gatttctgtt atttaggaac
      661 ctcaccaatt cttttacaag tgaaaaataa atacaataca gaaagcagtt cgagttcagc
      721 tataagagta ggaacaacaa ttagaaaatg gaatcgttta aataagtcga caaaaatttc
      781 tggtttggat agccctatcc ccaattggaa agcattaata gaaatgactg aagctagtaa
      841 ggaactggca gctaagttaa ctgaaaatag ttatttagct tatattaatg aaaaatccac
      901 acgagaatta tggacactcg atgactaaca atatccttat tagtatctat ccacgataca
      961 tggaaaaaat tataaacaaa caaaaaaact ttgaatttag aaatttctat cttacctcag
     1021 atccttataa tatcttttgg atttacgaaa caatccccac caaagcttta aagtataaat
     1081 tgattgtaaa aaatccaatt actaaactta agaaaaatca acactacttt ttaggagacg
     1141 atgagtttga acaattagtt taaaag
//
LOCUS       DADOOS010000293         1143 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.305, whole genome shotgun sequence.
ACCESSION   DADOOS010000293 DADOOS010000000
VERSION     DADOOS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.305"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="J8C37_002921"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="J8C37_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAZ5306304.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DADOOS010000294         1132 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.306, whole genome shotgun sequence.
ACCESSION   DADOOS010000294 DADOOS010000000
VERSION     DADOOS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.306"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            48..458
                     /locus_tag="J8C37_002922"
     CDS             48..458
                     /locus_tag="J8C37_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAZ5306305.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            472..828
                     /locus_tag="J8C37_002923"
     CDS             472..828
                     /locus_tag="J8C37_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAZ5306306.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          401 a          164 c          216 g          351 t
ORIGIN      
        1 aaagaaatta ctgtagaatt ggattataat taaaaatgag tgagaaaatg ataaaagtat
       61 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
      121 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
      181 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
      241 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
      301 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
      361 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
      421 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
      481 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
      541 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
      601 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
      661 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
      721 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
      781 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
      841 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
      901 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
      961 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     1021 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     1081 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gt
//
LOCUS       DADOOS010000295         1128 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.307, whole genome shotgun sequence.
ACCESSION   DADOOS010000295 DADOOS010000000
VERSION     DADOOS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.307"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>1128
                     /locus_tag="J8C37_002924"
     CDS             <1..>1128
                     /locus_tag="J8C37_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAZ5306307.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGT"
BASE COUNT          468 a           93 c          192 g          375 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaa
//
LOCUS       DADOOS010000296         1117 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.308, whole genome shotgun sequence.
ACCESSION   DADOOS010000296 DADOOS010000000
VERSION     DADOOS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.308"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..900)
                     /gene="vanY"
                     /locus_tag="J8C37_002925"
     CDS             complement(<1..900)
                     /gene="vanY"
                     /locus_tag="J8C37_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAZ5306308.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTN"
BASE COUNT          329 a          228 c          159 g          401 t
ORIGIN      
        1 attagtatgt gttgatccgg gaaacactgt cacaattaca ccgtctatat tgtttcctga
       61 tatctcataa cgaagattag tcggcacatg aatggtggta tttttagtaa caggataata
      121 aaagatctca tatttttccc catttacact aacagaaatg gttttttctt cttttaggta
      181 atccatatat tcctcgagaa cgaaattctt ttctttcata atcgcactat gtggtaaacc
      241 aacatagcga atatgccatg gttcatattg aattcctgtt aactctgttt tgtcctctgg
      301 ataacgtaaa atgaacccgt atttccaagc attttcttct atccactttc cttcaggggc
      361 tcgttccatt ttcgtcaagc ttgatcctac atctagtgat aaacctgaat tatgctcact
      421 ataacctgct ggtaaggcat actcagcccc catttcttgg taaagcacac tttgctcatc
      481 aaagtctcga tagccactat taataataaa atgactaacg ccacccttta cagcatcatt
      541 gaccatctct gaaaattttt gtgctatttc ttttgacata taaatattac tatcaagcaa
      601 cccgtatcca tttattaatt cgtcatgttt agataaattc acgatatctg acttcacact
      661 ttcttggcga acaggatatt tactattgat taatagcaga tttccttgat aaacctgttc
      721 ttctgtaatt gttttctctt gggtattttc agaagtccca ctattttcta aatgttcttt
      781 gggattttga tcataatttt gaaattcgac tttttcctga gaaaacagtg cttcattaac
      841 gtagtcataa cctaagtata ttaagaataa caataacaat aaaaaaaaca acttcttcat
      901 tttcagtctc ctccttaact atatagttgg aagtatgaaa tatttctaga atcttcccct
      961 taaacggcac aatctcgttt gagattgtgc cgttttgccc cgtaaattac agtcaattat
     1021 ttcgccctac tggatttact attatcacca atgtagatga attttcataa gtgataccca
     1081 tgctgaattt aacgaggctg aactgcctgt tgacaat
//
LOCUS       DADOOS010000297         1093 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.309, whole genome shotgun sequence.
ACCESSION   DADOOS010000297 DADOOS010000000
VERSION     DADOOS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.309"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>1093
                     /locus_tag="J8C37_002926"
     CDS             <1..>1093
                     /locus_tag="J8C37_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAZ5306309.1"
                     /translation="LFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGS
                     VMYLLNITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVT
                     IIANIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLN
                     YSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQI
                     SAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPF
                     LLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIAT
                     VICSYVIYVVRKRAVGKDIFIKHYHIVVES"
BASE COUNT          356 a          131 c          202 g          404 t
ORIGIN      
        1 tattgtttcc aattttaacc ttaaacattg tagatgctgt gatgcgcttt ttaatggata
       61 aagataaaaa taaaaaaaat atagcatcta tagcgattaa agttatattt ttaagcgtga
      121 tagtctttgg atccgtaatg tatttgttaa atattacaaa attatggcaa gatgtttatg
      181 gattagaaaa atatattttt ttgtattata tatcctatgt atggaatcaa tttctaatac
      241 aatttgctaa agggttagaa aaagttaaag ctatgggcat agcaggtatt atcagcacaa
      301 ttgtaacaat aattgccaat attttcttct tgcttattct gaaatgggga ttaattggtt
      361 ttttcattgc aaatatttta tcacagatta tttcagctac ttatctgtca gtattagtga
      421 gaatatggga atatatcgat tttgaggcta aagatcctca gctaaagaga gaaatgctaa
      481 attactcgat tccattaatt gcaactgttg ttggatggtg gattaatagt acagctgata
      541 ggtatgtggt ttcctttata gtaggtgtct cggcgaatgg cttattgtct gtggcatata
      601 aaatacctca aataattaat actttgcagg gaatatttat acaggcctgg caaatttctg
      661 caattaaaga atatggagat aatgagactg caaaatttta tggaaataca tttgttatga
      721 ttaatttgtt aatgtgcata acctgttcaa gtcttatcat attaactaga ccgttttcac
      781 atattcttta tgcgaaagac ttttatgaag cgtggaaata tgttccattt ttattaatag
      841 ctaatgtttt aaattgtgca tcaggtctgt taggacctat cttatcagca aaaaaagatt
      901 ctaaatccat gatgtggtct gcaatcatag gggcattagt taatgtgatt atgagtgtag
      961 tccttgttta taagataggg gttcaaggtg ccacaatagc cactgttata tgttcatatg
     1021 taatatatgt tgttcgtaaa cgagctgtgg gaaaagatat ttttataaag cattatcata
     1081 ttgttgtgga atc
//
LOCUS       DADOOS010000298         1069 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.310, whole genome shotgun sequence.
ACCESSION   DADOOS010000298 DADOOS010000000
VERSION     DADOOS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.310"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            82..>1069
                     /locus_tag="J8C37_002927"
     CDS             82..>1069
                     /locus_tag="J8C37_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAZ5306310.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPP"
BASE COUNT          361 a          204 c          207 g          297 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccc
//
LOCUS       DADOOS010000299         1062 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.311, whole genome shotgun sequence.
ACCESSION   DADOOS010000299 DADOOS010000000
VERSION     DADOOS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.311"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(37..993)
                     /locus_tag="J8C37_002928"
     CDS             complement(37..993)
                     /locus_tag="J8C37_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306311.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DADOOS010000300         1056 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.312, whole genome shotgun sequence.
ACCESSION   DADOOS010000300 DADOOS010000000
VERSION     DADOOS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.312"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            287..694
                     /locus_tag="J8C37_002929"
     CDS             287..694
                     /locus_tag="J8C37_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306312.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..>1056
                     /locus_tag="J8C37_002930"
     CDS             708..>1056
                     /locus_tag="J8C37_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306313.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNV"
BASE COUNT          394 a          133 c          181 g          348 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtat
//
LOCUS       DADOOS010000301         1043 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.313, whole genome shotgun sequence.
ACCESSION   DADOOS010000301 DADOOS010000000
VERSION     DADOOS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.313"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..>1043)
                     /locus_tag="J8C37_002931"
     rRNA            complement(<1..>1043)
                     /locus_tag="J8C37_002931"
                     /product="16S ribosomal RNA"
BASE COUNT          223 a          326 c          234 g          260 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caa
//
LOCUS       DADOOS010000302         1028 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.314, whole genome shotgun sequence.
ACCESSION   DADOOS010000302 DADOOS010000000
VERSION     DADOOS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.314"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            1..333
                     /locus_tag="J8C37_002932"
                     /pseudo
     CDS             1..333
                     /locus_tag="J8C37_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            367..>1028
                     /locus_tag="J8C37_002933"
     CDS             367..>1028
                     /locus_tag="J8C37_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAZ5306314.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLD
                     SK"
BASE COUNT          372 a          121 c          189 g          346 t
ORIGIN      
        1 tcaccaatta ttgattcgac aggtctatgt ttattagatg aattcttggc ggatatcagt
       61 ctatttataa tgtttattga tgtaattgaa gaaacatcaa gaaatttttt gctgtctaag
      121 ttcagaaaat ttcgtatgaa aaatgataaa caaaaattat tggcaaatga gatttggagt
      181 agtttagaag tcgaagaaag taaacaagaa cttttgttga aatggatact ttttcttgta
      241 aagaagttct atgtgaaaat accagaaacc cattgtctga tagttatgca cggtaaaata
      301 acagcttctg cgatcgcaag ggaaacaaat aagttgctga atacatatgt ttatgaggct
      361 ttcgatatgc caattgaagg tgagactagt gacttaatta acctaatcaa caatttttgt
      421 aaaagtatag atacgacgaa tggactcatt ctgctagttg atatgggatc attggaacaa
      481 atgtatgaaa aaattgaatc caatgtaata ggtgatttgg taattttaaa caatgtttct
      541 acagctttag cgattgaatg tgggattcaa gtttgccaaa aaaagccaat tagtcatttt
      601 taccagatgg attttgattc gtttcaagtt aaagttcagt attataaagg attatcacaa
      661 aagaaaaatg taatagtatc ttgtctctct ggtgagggga tttctgaaaa agttaaggat
      721 attttaaaac ggtatctcga taatcaagtg gagattttga tttttgattt taacgcatta
      781 aagaaaatag caaaggaaaa agatactatg atttttaaga atacaatttg tgttctttca
      841 accactgaat tttacattca aggaatcgat tgtttaaatt tagaaaatct aattaatggg
      901 aatcaaacct tagaaaaatt aaataagtat atggactcgg atagctgtga gttctgctta
      961 aatgaattag ttaaactctt tactattgaa ggcgctagta ctaagcttcg ctttttagat
     1021 tccaaaaa
//
LOCUS       DADOOS010000303         1027 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.315, whole genome shotgun sequence.
ACCESSION   DADOOS010000303 DADOOS010000000
VERSION     DADOOS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.315"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..386
                     /locus_tag="J8C37_002934"
     CDS             <1..386
                     /locus_tag="J8C37_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAZ5306315.1"
                     /translation="KYPFFVFEDLASFQKKYSIVLYKYLAMYYKKYLIAKNKGDFEKA
                     EIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSMLNIDFAKVRKGR
                     NYNFAVQFNISSEFDEKTYREKKLG"
     gene            578..976
                     /locus_tag="J8C37_002935"
     CDS             578..976
                     /locus_tag="J8C37_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAZ5306316.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          375 a          120 c          201 g          331 t
ORIGIN      
        1 agaagtatcc gttctttgtt tttgaagact tagcatcttt tcaaaaaaaa tatagcatcg
       61 tgttatacaa atatttggcg atgtattata aaaaatatct tatcgccaaa aataaaggag
      121 attttgagaa agctgaaata tataagaatc ccctgattga aatagttaaa attcgtgaga
      181 ttacgaaaac agaaaatgag tataaaacaa tatcgaattt tgatagacgt gttttaaggg
      241 aatcagttga ggaaattaat gaaaaatcaa tgctgaatat tgattttgct aaagttagaa
      301 aaggcagaaa ttacaatttt gctgtccagt ttaatatttc tagtgagttt gatgaaaaaa
      361 cttatagaga aaaaaaatta ggttaatgca aaatatatct tactcgtttg aagatgcttc
      421 agacgtatgc taagatatat agtgccttgg gagcaaggta ttaatctcct ttagaggtta
      481 attagcttgt gatcgcctat caattttttt ggtaggcgat tctttttttt gaaacaaaaa
      541 agcaaaaatt attttttaat tagagaggtg caatgcgatg aaatactttt tttgtgcagt
      601 attcgaatgg aaaggaaatg aatatgcagt taaattccca gattttccag acttatatac
      661 acatggtgaa actttagaag atgcttttga gatggcgcaa gaagtattgg aaggatactt
      721 ggtgctttta gagttgcaag aaaaagaaat tccatcgcct acgcctgttc aagagttaaa
      781 tgcaagaatc aagcgatctg agaatgttaa tttatttgtc gtaaaagcta atacgaaagt
      841 tgctaaagct cgaaacaagc aaaattatga tcgaaaatca gtgactgtgg aacactattt
      901 aaatgtttta gcggctgaag agaatattaa tctgtcgaaa gctttgaatg atttgttgag
      961 agaaaagttg atttgatgtc tctcagatga tcatattaag caaaaacagc ttgttttagg
     1021 acgtatg
//
LOCUS       DADOOS010000304         1015 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.316, whole genome shotgun sequence.
ACCESSION   DADOOS010000304 DADOOS010000000
VERSION     DADOOS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.316"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..944
                     /locus_tag="J8C37_002936"
     CDS             <1..944
                     /locus_tag="J8C37_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAZ5306317.1"
                     /translation="HYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIA
                     AGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVG
                     EAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNN
                     PSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATF
                     TKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYR
                     TYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          365 a          127 c          176 g          347 t
ORIGIN      
        1 atcattattt tggaaggaat tttgattatg aaatatctta caatgaagta gttactgtta
       61 ctccaagaaa ttataagttg aattttcgaa aggtaaatga tttggatact cattatgcaa
      121 tgattggtat tgccgctggt atagctgact accctcttta ttacgatgcg acaaatgaaa
      181 aaggattgag tatggctggg ctaaattttt ctgggtatgc tgattataaa gaaatacaag
      241 aagggaaaga caatgtatct ccttttgaat ttattccttg gattttagga caatgctcaa
      301 cagtaggaga agctaaaaaa ttgttaaaaa atatcaattt agcaaatata aattatagtg
      361 acgaacttcc tttatcccct ttacattggc tattagctga taaagaaaaa tcaattgtca
      421 ttgaaagtat gaaagatgga cttcatatat atgataaccc tgtgggcgtt cttaccaata
      481 atccttcatt tgactatcaa ttatttaatt taaacaatta tcgtgtctta tcgagtgaaa
      541 ctcctaaaaa taatttttca aatcaaataa gtttgaatgc ctatagccgc ggtatgggag
      601 ggataggctt gcctggagat ttatcctcag tatctcgttt tgttaaagcg acttttacga
      661 agctgaattc tgtatctgga gattcagagt cagaaagtat tagtcaattt ttccatatct
      721 taggttcagt agaacaacaa aaaggtttgt gtgatgtagg tgatggaaaa tatgaatata
      781 caatttattc ttcttgttgc aatgttgaca aaggaatcta ttattatcga acatatgaag
      841 acagtcaaat tactgcaatt gatatgaata aagaagactt agatagtcat aagttaatta
      901 gttatccaat tatagaaaaa caacaaatta aatatataaa ttagttaatg tgttgtgatt
      961 gattatttaa tatagatata atacaaaaag gcaaagatat tttcaaaaca ttttt
//
LOCUS       DADOOS010000305          991 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.317, whole genome shotgun sequence.
ACCESSION   DADOOS010000305 DADOOS010000000
VERSION     DADOOS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.317"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(21..94)
                     /locus_tag="J8C37_002937"
     tRNA            complement(21..94)
                     /locus_tag="J8C37_002937"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="J8C37_002938"
     tRNA            complement(108..181)
                     /locus_tag="J8C37_002938"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="J8C37_002939"
     tRNA            complement(209..281)
                     /locus_tag="J8C37_002939"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="J8C37_002940"
     tRNA            complement(301..374)
                     /locus_tag="J8C37_002940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="J8C37_002941"
     tRNA            complement(384..457)
                     /locus_tag="J8C37_002941"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="J8C37_002942"
     tRNA            complement(468..553)
                     /locus_tag="J8C37_002942"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="J8C37_002943"
     tRNA            complement(570..641)
                     /locus_tag="J8C37_002943"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="J8C37_002944"
     tRNA            complement(653..725)
                     /locus_tag="J8C37_002944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="J8C37_002945"
     tRNA            complement(743..824)
                     /locus_tag="J8C37_002945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="J8C37_002946"
     tRNA            complement(832..904)
                     /locus_tag="J8C37_002946"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="J8C37_002947"
     tRNA            complement(908..980)
                     /locus_tag="J8C37_002947"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DADOOS010000306          987 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.318, whole genome shotgun sequence.
ACCESSION   DADOOS010000306 DADOOS010000000
VERSION     DADOOS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.318"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(26..913)
                     /locus_tag="J8C37_002948"
     CDS             complement(26..913)
                     /locus_tag="J8C37_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAZ5306318.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          285 a          206 c          179 g          317 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattat
//
LOCUS       DADOOS010000307          967 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.319, whole genome shotgun sequence.
ACCESSION   DADOOS010000307 DADOOS010000000
VERSION     DADOOS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.319"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            334..967
                     /locus_tag="J8C37_002949"
                     /pseudo
     CDS             334..967
                     /locus_tag="J8C37_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          328 a          181 c          177 g          281 t
ORIGIN      
        1 taaattttta ccagaaagaa atgaacacct tcaattatct aaatctcgaa aatggttaga
       61 tgtaatcatc aggaaaaaca atcttaaacg aattactatt catggtctta gacacacaca
      121 tgctagcttg cttttagcaa gctagtgcaa atattaaaga tgtgcaagag cgtttaggcc
      181 actcttctat tcaaatcatt atggatctat atattcatat tacagacaaa agaaaagaag
      241 aaaccgtagc gcaattcgca aaatataggc tctataattt atgggactga tgaatcaaaa
      301 ttgattcttc agtcccattg ttattttagg tatatgattc gtacattgaa agagttgaaa
      361 aactatcttc cgaagccgtc aaaaaggacc ctgccaaaag tattgatggt ggatgaattt
      421 cgttcgcacg cttcaattgt ggataaaatg agctttattt gtgcggatgg cgagactgga
      481 aaactcattg atattttgcc tacacgaaaa ttgtctcgat taacacacta ttttagtcgc
      541 tgtgcaaatc ctgaagcggt ggaatattta gtgacagata tgaacgccgc ctacttccaa
      601 ctgacaaaac gtgtcttacc aaatgcgaaa gtcgtcattg attgttttca tatcatcaaa
      661 catttgaatc aggcgttcaa tgattttcgt gtacaagaaa tgaaaagatt acaccagtta
      721 gggcaacgac gggaagccga gaaagtgaaa aagaactggc gctttcttct aaaaaaccgt
      781 caaaaaatca atcactatga ttacaaaaca tggcgcagtt ttcgtgcacc taaatacccg
      841 ctactcactg aagcaatgat gattgatcga ttgctggcat tttcaacgcc tttaaaggac
      901 gcctatactt ttttccatga gctgaccgaa gcctttagag acaaggaatc agacttattt
      961 tttacct
//
LOCUS       DADOOS010000308          948 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.320, whole genome shotgun sequence.
ACCESSION   DADOOS010000308 DADOOS010000000
VERSION     DADOOS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.320"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>948
                     /locus_tag="J8C37_002950"
     CDS             <1..>948
                     /locus_tag="J8C37_002950"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAZ5306319.1"
                     /translation="FVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLN
                     DVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFF
                     SVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLR
                     NGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAF
                     LLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSI
                     PTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI"
BASE COUNT          297 a          164 c          214 g          273 t
ORIGIN      
        1 cctttgtgca attggcatct acctacaatc aatttaatgg gttatttgat gatgtggatc
       61 tccaatcaaa aaaactagga acagatgagc aacaacgaaa tgtgacgatt accgaagtta
      121 tcaagaaatt gaatgacgtg gaagttttag gtcacgatgg ggatgtgatc ggggatgcgt
      181 atgaatttct gatcagtcaa tttgcttcag aagcagggaa gaaagccgga gaattttaca
      241 cgcctcatat ggtttctgat atgatggcac aaatcgtgac gttggatcaa aaagaacgac
      301 gattctttag tgtctttgac ccaaccatgg gttcaggttc cttgatgtta aatgtacgta
      361 actacttaac tcatccagat aacgttaaat atcatgggca agaactaaat acaacgacat
      421 acaatctagc aaaaatgaac ttgattttac atggggtaga tgccgaagaa atgaatctcc
      481 gcaatgggga tacgttgaat aaggactggc caacagatga accttacaca tttgatgcag
      541 tagtgatgaa tccaccttat tcggctaatt ggtcagcaga tacgaccttc ttagatgatt
      601 ctcggtttaa tcgctacggg aaattagcgc caaaatccaa agcagatttt gcgtttcttc
      661 ttcatggttt ctatcatttg aaggaaacag ggactatggc tatcgtcttg ccacacgggg
      721 ttttattccg tggtgctgca gaaggggtca ttcgtcaaaa attattagaa gacggcagta
      781 tttatgctgt gatcggtatg cctgcgaact tgttctttgg tacatcgatt ccaacgacgg
      841 tgattgtatt gaagaagaat cgtcaaactc gagatgtatt attcattgat gctagtcgtg
      901 aatttgttaa agggaaaaat cagaataaat tatcggaaga aaacattc
//
LOCUS       DADOOS010000309          946 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.321, whole genome shotgun sequence.
ACCESSION   DADOOS010000309 DADOOS010000000
VERSION     DADOOS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.321"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            86..355
                     /locus_tag="J8C37_002951"
     CDS             86..355
                     /locus_tag="J8C37_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAZ5306320.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="J8C37_002952"
     CDS             348..605
                     /locus_tag="J8C37_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAZ5306321.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          359 a          101 c          177 g          309 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag
      901 caataggttt aataatctat tttaaatttt ttgtactagt tttagt
//
LOCUS       DADOOS010000310          931 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.322, whole genome shotgun sequence.
ACCESSION   DADOOS010000310 DADOOS010000000
VERSION     DADOOS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.322"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..865
                     /locus_tag="J8C37_002953"
     CDS             <1..865
                     /locus_tag="J8C37_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306322.1"
                     /translation="IVVPKNKSEDGNYELEVLQFKIEQKSSERFMSNQLVKIKKWLPM
                     IDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYK
                     GDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSK
                     SNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERD
                     SYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKI
                     YKEIIEQYFPK"
BASE COUNT          384 a           95 c          160 g          292 t
ORIGIN      
        1 gatagttgtt cccaaaaata aatcagaaga tgggaattat gagttagaag ttttgcaatt
       61 taagattgaa caaaagtcca gtgaaagatt tatgagcaat caattagtga aaatcaaaaa
      121 atggctccct atgattgatc aattagtatc taataataag gaagctaaaa atttatgtat
      181 ttttgtctta ttattacatg gaagagttat taacttagtt aatcgaaaaa gtacgactaa
      241 atttgcaaac tcattctggt ttttaaaaaa tattaataaa acagtggaaa agaatggagt
      301 ttataaaggt gattttcatt ttcaaaatgc tgagactatt gaacgagaac atcattattt
      361 cgatttatta gtgaatacag tggtagaatt agcgaaatgg gcaaagtcaa acgaaactta
      421 tagtcaagta cttaaaagaa ttatgagtca agaaacttca tttatctatg agttaagcaa
      481 gtcaaatttg aaatatactt ataatgaaga tgaacttgaa attttagata atccagaaaa
      541 ttcacaatgg attaaagagt tgaagagaga agactttatt gagacactaa gaattctaaa
      601 atcgttattt gatttgaaat tagccaaagc taccatcatg ggaattgaaa gagattcata
      661 ttattgggag gaaattggac tatatgctcc ccagattgaa ggtgaagata aagagaaaag
      721 gatagacaac attattaact atattaatag tttaagtgaa cctttaaaat gtagaggact
      781 tgattttata ggaacattag gatggacaga aacatactat gagaaaatat ataaagaaat
      841 aattgaacaa tattttccta aataatttaa taaggattga tttatcgtaa aagaaaaaag
      901 ttgtgatttc caaaatcaca acttttttct t
//
LOCUS       DADOOS010000311          929 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.323, whole genome shotgun sequence.
ACCESSION   DADOOS010000311 DADOOS010000000
VERSION     DADOOS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.323"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>929
                     /locus_tag="J8C37_002954"
     CDS             <1..>929
                     /locus_tag="J8C37_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAZ5306323.1"
                     /translation="IYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPDASGEIADEYQ
                     KKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYLVASKENA
                     DIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKDQEVEAFPMSV
                     CMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCYRKEEQESITK
                     SLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVDDANLGIKEKR
                     KYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHK"
BASE COUNT          372 a           95 c          187 g          275 t
ORIGIN      
        1 aatttacaat gttgaaaaat atttaagaaa atgtgtggat agtttattga gtcagacatt
       61 aaaagagata gaaatcgtat tagttgatga tggttcgcca gatgcttcgg gggaaatagc
      121 agatgagtat caaaaaaaat attctaatgt aaaaacaata catagagaaa atggggggtt
      181 aggaccggca agaaatacag gaattgagaa tgcgacagga gaatatgtgg cttttcttga
      241 ttcagatgat tgggtagaaa gtgatatgta tgaaaaatta tacttagttg cgtccaaaga
      301 aaatgctgat atagtagtta gtggacattg tgattttgcg aatgacaaag ctatggtgaa
      361 aaagcaacat cctttgagtg ggaatgtata tgatacgcct aatagtatta aaaatgtacg
      421 aaaaaattta tttggacata gtccaaaaga tcaagaagta gaggccttcc cgatgtcagt
      481 atgtatgtct ttgtataata aaaatctgct agataaatat agactaagat ttgaaaaaat
      541 tttatctgaa gatacaattt ttaacttgaa tgcatatgat gtcgcaaata aaattgcttt
      601 tacagaatat acagattatt gttatagaaa agaagaacaa gagtctatta caaaaagttt
      661 atctccaaat aagcagacgg aatttaaaga atttttaata actctaaaag ctatagcatc
      721 gaaagaagat ttagaatgtc aaatcagagc aaaacgtatg gcaattgatt attgtaggct
      781 ttatgtgggc ttagttgatg atgcaaattt gggaattaaa gagaaaagaa agtatataaa
      841 aaaatttatt gaagatgagg gattaattca atgttggaag ggatatccta caaatacgct
      901 accatttcaa caaaaatttt ttcataaaa
//
LOCUS       DADOOS010000312          919 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.324, whole genome shotgun sequence.
ACCESSION   DADOOS010000312 DADOOS010000000
VERSION     DADOOS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.324"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>919
                     /locus_tag="J8C37_002955"
     CDS             <1..>919
                     /locus_tag="J8C37_002955"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAZ5306324.1"
                     /translation="LASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVD
                     VLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVF
                     DPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGD
                     TLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLH
                     GFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTT
                     VIVLKKNRQTRDVLFIDASREFVKGKNQNK"
BASE COUNT          293 a          163 c          205 g          258 t
ORIGIN      
        1 attggcatct acctacaatc aatttaatgg gttatttgat gatgtggatc tccaatcaaa
       61 aaaactagga acagatgagc aacaacgaaa tgtgacgatt accgaagtca tcaagaaatt
      121 gaatgatgta gatgttttag aacacgatgg ggacgtgatc ggggatgcgt atgagtttct
      181 gatcagtcaa tttgcttcag aagcagggaa gaaagccgga gaattttaca cacctcatat
      241 ggtttctgat atgatggcgc aaatcgtaac gctcgatcaa aaggaacgac gattctttag
      301 tgtttttgat ccaacgatgg gttcaggttc attgatgtta aacgtacgta actacttaac
      361 acacccagat aacgttaaat atcatgggca agaactaaat acaacgacat acaatctagc
      421 aaaaatgaac ttgattttac atggtgtcga cgccgaagaa atgaatctcc gcaatgggga
      481 tacgttgaat aaggactggc caacagatga accttacaca tttgatgcag tagtaatgaa
      541 cccaccttat tcggctaatt ggtcagcaga tacgactttc ttagatgatt ctcggtttaa
      601 tcgttatggg aaattagcgc caaaatccaa agcagatttt gcgtttcttc ttcatgggtt
      661 ctatcatttg aaggaaacag gtaccatggc catcgtctta ccacacggag ttttattccg
      721 tggcgctgca gaaggggtca tccgtcaaaa attactagaa gatggcagta tttatgctgt
      781 gatcggtatg cctgcgaact tgttctttgg gacatcgatt ccaacgacgg tgattgtgtt
      841 gaagaagaat cgtcaaactc gagatgtatt attcattgat gctagtcgtg aatttgttaa
      901 agggaaaaat caaaataaa
//
LOCUS       DADOOS010000313          884 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.325, whole genome shotgun sequence.
ACCESSION   DADOOS010000313 DADOOS010000000
VERSION     DADOOS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.325"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(630..875)
                     /locus_tag="J8C37_002956"
     CDS             complement(630..875)
                     /locus_tag="J8C37_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAZ5306325.1"
                     /translation="MIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEG
                     YKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          295 a          168 c          126 g          295 t
ORIGIN      
        1 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
       61 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
      121 aaaacaagcc cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg
      181 tatatttttt tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc
      241 ctactagtta gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt
      301 atctgaaaca gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt
      361 cagatacatt tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag
      421 cctatgcttt ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat
      481 gaatgaaaaa ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat
      541 gtgaacaaat aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg
      601 aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac
      661 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc
      721 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
      781 ttaggaattg gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca
      841 gaaacaaacc actcattgga aaagacatga atcaaatcat cact
//
LOCUS       DADOOS010000314          871 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.326, whole genome shotgun sequence.
ACCESSION   DADOOS010000314 DADOOS010000000
VERSION     DADOOS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.326"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..705
                     /locus_tag="J8C37_002957"
     CDS             <1..705
                     /locus_tag="J8C37_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAZ5306326.1"
                     /translation="NLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGII
                     PSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLK
                     VRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRE
                     SFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVS
                     PKQYQLGLVDNFNNIS"
BASE COUNT          316 a          100 c          156 g          299 t
ORIGIN      
        1 aatctgttat cacaaaatta cgatccagaa atatatctgt ttctttatga aaataaaata
       61 ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt taatctgagt
      121 aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa ttattctatc
      181 attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat aatagaagcg
      241 tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt acctgaggtc
      301 ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa aactaagata
      361 gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa tatctataaa
      421 agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa attaagagaa
      481 agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg aaaaatttct
      541 acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt aggtttagga
      601 ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt atctcctaag
      661 caatatcaat taggtttagt ggacaatttc aacaacatta gctgagaggt tttattttat
      721 tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt ttaacaaaaa
      781 atttttttgg gaaatttctg atttgagttg tttcgtagaa tctgagcgtc taagtaatgg
      841 taataatagt gtgttaaatg aatatgacaa a
//
LOCUS       DADOOS010000315          870 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.327, whole genome shotgun sequence.
ACCESSION   DADOOS010000315 DADOOS010000000
VERSION     DADOOS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.327"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            51..>870
                     /locus_tag="J8C37_002958"
     CDS             51..>870
                     /locus_tag="J8C37_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAZ5306327.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          301 a          131 c          159 g          279 t
ORIGIN      
        1 tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat atgaacgatt
       61 ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat ttgtttcgtc
      121 gtcacctaga attggctatt aacgctttat tacaagctga attaacggta tttcttgact
      181 atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg aattattcgc
      241 gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat aggaatggag
      301 acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta gaaaccacta
      361 ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta atcgaaaaaa
      421 tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt gtgtctgaag
      481 atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt tttatggatg
      541 ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat atagtaattg
      601 gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca actgaatctg
      661 cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa gaggttttgt
      721 tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt tatccaaatg
      781 ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag gttcgtgtta
      841 gtgatcgaca agaaatctgt agcgatttca
//
LOCUS       DADOOS010000316          847 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.329, whole genome shotgun sequence.
ACCESSION   DADOOS010000316 DADOOS010000000
VERSION     DADOOS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.329"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            93..581
                     /locus_tag="J8C37_002959"
     CDS             93..581
                     /locus_tag="J8C37_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAZ5306328.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          329 a           68 c          143 g          307 t
ORIGIN      
        1 ataaaatagt atttcttata ttgtatagtt tttgttttta tgtgaaaaca tgagcaatag
       61 aattgtacta gtaggataag gatggatgga aaatgaaaaa aactattttt gttttagcga
      121 tttttatatg ttttgggcta cttttggtag gctacttgtt gtttaattct aatgaaatga
      181 gaactatttc tacgaatgac ttgttaaaaa aaagtgaatt aaaggataaa aaaaaagaat
      241 ctcttgcctt tataatgttt actcaagaag agtgtgagta ttgtatagca ataaatccaa
      301 tcatagatag tgtaaataag gaaactttga ataagataaa gaaatatgat atatctgtag
      361 attttcaatc taaacaaaag tgtaaaatta agtttgttcc gactattgtt gcatatagta
      421 agaataaaga agtggggaga attgacaatg aattagataa tttgtatcga aaaaagcaag
      481 atggttcaat tgtaatagac aagaaaaaaa caaagacatt gcttatagat tggatgaaaa
      541 aaatggataa acaaggagaa tatttgtatg gagaagatta aacagacatt tccgttaatt
      601 aataaatatt ggaataagaa aaaaattttt atatttttta ttacgtttct gctgtcatct
      661 ttattttctc taattttccc gtatctattt agtaatgtta ttgatttata tttagagaat
      721 gagttatcac agaatattgt gcgaatagta acgattctta tatttcagtt atttttagtc
      781 ttaattaaag aagtgtatgg atatataaaa actatttatg catttgaagt tgatttatca
      841 atgaaaa
//
LOCUS       DADOOS010000317          842 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.330, whole genome shotgun sequence.
ACCESSION   DADOOS010000317 DADOOS010000000
VERSION     DADOOS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.330"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>842
                     /locus_tag="J8C37_002960"
     CDS             <1..>842
                     /locus_tag="J8C37_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAZ5306329.1"
                     /translation="IQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKN
                     VLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSS
                     EIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVA
                     NQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSF
                     PRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMN
                     ISKFS"
BASE COUNT          309 a          106 c          148 g          279 t
ORIGIN      
        1 atccaattaa catcggacga taaattatat tccttcatca atgatattaa tagttggtgt
       61 agtcgagaag ttcaattagt caatcgtttt attcaaatag gggatattta tttaacaaaa
      121 attcaaaaaa atgttttggt tattttattt aagttaatgt gtagtaaaga atttcagcaa
      181 aaacgtttag cactgatttt ggctcatgga gatctaacgg caacctgtat agcaagtact
      241 gctaatagga tcgttgagag atatatatac gacgccattg atatgccact aatgacttca
      301 agtagtgaga ttgcagacaa aattaataga tacttacaag agtgtaccgg atatgaagaa
      361 attattttac ttgttgatat gggttcactg gagaaaattt atctaaattt ggaactagac
      421 gctcaaaaaa aattcggact actaaacaat gtttcaacaa gaatggctat tgatgttgca
      481 aatcagatat tattagattt accgcttaaa cagattcttg aacaagtaaa agaaaatcaa
      541 aatgtggatt atatttatga atcatcaaat attaagaagg atatgattgt ctgttcttgt
      601 gcaagcggat taggaacttc gtttaaatta aaaaagatac ttgaagatag ctttccaagg
      661 gatactgatc ttgtcataga aacgtgtgac tattactctt tagtgaatga aaactattta
      721 tcggagcttc gtaaagagaa caatattctt tttattgttg gaacactaaa tccacatcta
      781 gaagatgtag aattcttttc aattgaagat atgattttag gaatgaatat aagtaaattc
      841 tc
//
LOCUS       DADOOS010000318          798 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.331, whole genome shotgun sequence.
ACCESSION   DADOOS010000318 DADOOS010000000
VERSION     DADOOS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.331"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(24..>798)
                     /locus_tag="J8C37_002961"
     CDS             complement(24..>798)
                     /locus_tag="J8C37_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAZ5306330.1"
                     /translation="FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIK
                     WMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHS
                     SDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKR
                     AHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILS
                     IFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          306 a          141 c           97 g          254 t
ORIGIN      
        1 aagcaatgaa agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt
       61 cccaaataat accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc
      121 tccaccattt catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca
      181 taattcactt tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa
      241 gcatagatac atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta
      301 tgcatgtttc catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt
      361 ttcaaaaatt ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa
      421 gttggtctta taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg
      481 gagttatcac tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat
      541 tcatttgact ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa
      601 tccttagcat aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc
      661 atccatttga tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca
      721 aacaacaaaa gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa
      781 gaaagtgtaa tataaaat
//
LOCUS       DADOOS010000319          790 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.332, whole genome shotgun sequence.
ACCESSION   DADOOS010000319 DADOOS010000000
VERSION     DADOOS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.332"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="J8C37_002962"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="J8C37_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAZ5306331.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          207 a          175 c          123 g          285 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt
//
LOCUS       DADOOS010000320          779 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.333, whole genome shotgun sequence.
ACCESSION   DADOOS010000320 DADOOS010000000
VERSION     DADOOS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.333"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            169..>779
                     /locus_tag="J8C37_002963"
     CDS             169..>779
                     /locus_tag="J8C37_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAZ5306332.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSID
                     SELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILL"
BASE COUNT          251 a          105 c          110 g          313 t
ORIGIN      
        1 attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta
       61 actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt
      121 tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt
      181 accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg
      241 agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct
      301 cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg
      361 gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat gactatggta
      421 atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta
      481 attattaaat taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt
      541 cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa
      601 aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc agaattacta
      661 tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt taactttaaa
      721 aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat tctactttg
//
LOCUS       DADOOS010000321          773 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.334, whole genome shotgun sequence.
ACCESSION   DADOOS010000321 DADOOS010000000
VERSION     DADOOS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.334"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..128)
                     /locus_tag="J8C37_002964"
     CDS             complement(<1..128)
                     /locus_tag="J8C37_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5306333.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYS"
     gene            complement(334..633)
                     /locus_tag="J8C37_002965"
                     /pseudo
     CDS             complement(334..633)
                     /locus_tag="J8C37_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171333.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
BASE COUNT          228 a          161 c          121 g          263 t
ORIGIN      
        1 gaataaacat tataaatggt ttgtctagag cgatttaaac tttgtgcgac aagagcgaca
       61 ggtttctcga tattgaaata agactctatg attactagtt cgtctgttgt aagatgggtg
      121 taggtcattt gtactcactc cttattaatt ctttggtcgg aactattttg agtgtagcac
      181 aaatgatctt tttagttgtc tagcttaatt ttacaatcga cgcctcaaaa agaatacccg
      241 aaatttaaac agcaaaaaaa gatagcgctt accattgtgt ttaagataaa acgctagcta
      301 agttggctag cgctttatta atctaagcaa gctctaaaat tgcttcaata caaaatcaac
      361 tgctccttta ggatcacgtg ttaaattgat atattctgca cctttagttg caattacttt
      421 tatccctaat ttgtctgcat ctttacggag atcatccaaa taggaattca cttgaggtgc
      481 tagaacgatt aaatcaaaat ttttcatcat atcataatga gaaccataag ctcctgcttt
      541 agcagaaaga ttaacacctg tctcttttgc gccctcttct aatgcattag ccaacattgc
      601 actcgttccg gcaccagcac aaagtactaa aacgcgcttt ccttcaccaa ccgatgcttt
      661 tgcttcatca actataactg ccgcagttgc accaacttct tggatttctt cgaccaattc
      721 ttcttttact tcttctgcag caatgttttt taactcttgt tctactaatt cag
//
LOCUS       DADOOS010000322          765 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.335, whole genome shotgun sequence.
ACCESSION   DADOOS010000322 DADOOS010000000
VERSION     DADOOS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.335"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            509..>765
                     /locus_tag="J8C37_002966"
     CDS             509..>765
                     /locus_tag="J8C37_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306334.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNY"
BASE COUNT          299 a           86 c          137 g          243 t
ORIGIN      
        1 tttcgttagt gtattaatgg gtgaaagttt aatcctaata ctgattttgt gtttaattgt
       61 tcatgaagtg aataaacaat tgtgaattaa aaaaataaaa acctagtata atcactttaa
      121 taaattgttg gtagaaggtg atcaaaatgg gagtaaacga aatgatgaaa gtcagattga
      181 gtttttaaat gataccttcc tcagaacttc aaaacgaatt caaagattcg ttgacaaatc
      241 ttgagcaaga tcatttaaaa aaatattttc tcttcatttg acgaaaaatg tttagaaatc
      301 ttatataagt gaagataaag taattggtgt aattaattgt agagaatagc ctttgagcaa
      361 ttgaagcgat acaattattt agaaaatgaa cttcctccag catactacat gattccttac
      421 gggacgatgt taaagaagaa agcaagaaat tttagaagta ttagtagata aactaatggg
      481 cttttctcaa tttaagggag tagcccaaat gtttatcaaa tatggtgcag atacagtagc
      541 aaccatgttt acagaagaat atcaagtaga ctattatgaa gtaatagata aatgtgtaca
      601 ttttgtttat gaaaaagtgt ggaattttat gaattttgcg ataagtgaag aaatcttacg
      661 agtacaagaa aacagaacag gaatatttac cagagacaga aagcttaatt tgattgcgca
      721 ttatatacgg aatcgctggt catcaatacc aaattttaat tataa
//
LOCUS       DADOOS010000323          753 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.336, whole genome shotgun sequence.
ACCESSION   DADOOS010000323 DADOOS010000000
VERSION     DADOOS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.336"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..473)
                     /locus_tag="J8C37_002967"
     CDS             complement(<1..473)
                     /locus_tag="J8C37_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306335.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKE"
BASE COUNT          235 a          122 c          115 g          281 t
ORIGIN      
        1 ttttctttga tgatgacatc gtctacagct tgctgccagt ttgatacatt tttagtaaat
       61 aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa ctgtgtcacg
      121 attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga ttctttgatg
      181 tcgtaatcat ctgctgaaag tttgacagag gatagcttat ttgacaaact tgtgattgtt
      241 tcttctgtta tttcagggtt tagaaaaatt ttttcttttg tactatacag cttgtctact
      301 tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc tgcataattg
      361 atccgttgct gatgttgccg ctgattattg atatggtatc caaacaaaca gatcgcaata
      421 gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc catagattca
      481 ctcctaagta ttgatgatag ataaattaag atataattca cttacataaa aatgacagct
      541 ttttatttaa aaaaacataa ttaaactaat atattcacag aaatatttgt aaacaagttt
      601 tccctatata atcataccac aaactgtttt cttgactagg gcaatttacg atttaaaagt
      661 aaataaaaca ttaataagat aaaaattaat cgatttcatt tttatttcca taattttcat
      721 aggtgttttt aaaagttaca caaaaaatag aat
//
LOCUS       DADOOS010000324          742 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.337, whole genome shotgun sequence.
ACCESSION   DADOOS010000324 DADOOS010000000
VERSION     DADOOS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.337"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            178..381
                     /locus_tag="J8C37_002968"
     CDS             178..381
                     /locus_tag="J8C37_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAZ5306336.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="J8C37_002969"
     CDS             381..731
                     /locus_tag="J8C37_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAZ5306337.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DADOOS010000325          714 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.338, whole genome shotgun sequence.
ACCESSION   DADOOS010000325 DADOOS010000000
VERSION     DADOOS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.338"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>714
                     /locus_tag="J8C37_002970"
     CDS             <1..>714
                     /locus_tag="J8C37_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAZ5306338.1"
                     /translation="QKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDIQV
                     MNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNCIW
                     VCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGIIT
                     RTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASGYF
                     AGYSLACNQESRWMFAIIRD"
BASE COUNT          280 a           92 c          133 g          209 t
ORIGIN      
        1 cagaaacatt caagtataaa aagaattata catcgcatat ccgaagaatt gaatgcaaca
       61 agacgaattg caaagataac atcttggaag gaagcatttg ccacgttaca agctaaaatt
      121 gatattcaag tcatgaatcg taatggatat aatgaaagta aaaaacagaa ggaacattta
      181 ttaaaaaaac atgaggtaat gctgaagtat tatgaaaaga ctttttcaga ttttttgaaa
      241 acatatgatt atagtcaaaa tcaaataatt aatcaatcaa aatcaccata ttctaactgc
      301 atttgggttt gttggtggca gggattggat caatcgccgg aaattgtaaa agcttgtgta
      361 aaatcaataa aacgaaatgc agggaaacat actgttataa ttttaacaga agataattat
      421 agacaatatg ttgatatacc aaaatgggtg gaagaaaaga aaaacaaagg aatcattacg
      481 agaacaaatt attctgattt attaagatta tctttgctag caaaacatgg aggtttgtgg
      541 ttagacgcca cattttattg tacaaaacca ataccagatg agtattttga tatcgatatt
      601 tggtctatta ggaggcctga ttatgctcac gcatccgtag catcaggata ttttgcgggt
      661 tattcattag catgtaatca agagtctaga tggatgtttg ctataattag agac
//
LOCUS       DADOOS010000326          712 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.339, whole genome shotgun sequence.
ACCESSION   DADOOS010000326 DADOOS010000000
VERSION     DADOOS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.339"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..446)
                     /locus_tag="J8C37_002971"
     CDS             complement(<1..446)
                     /locus_tag="J8C37_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAZ5306339.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DADOOS010000327          697 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.340, whole genome shotgun sequence.
ACCESSION   DADOOS010000327 DADOOS010000000
VERSION     DADOOS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.340"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>697
                     /locus_tag="J8C37_002972"
     CDS             <1..>697
                     /locus_tag="J8C37_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ5306340.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLES"
BASE COUNT          235 a          113 c          138 g          211 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgat
//
LOCUS       DADOOS010000328          697 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.341, whole genome shotgun sequence.
ACCESSION   DADOOS010000328 DADOOS010000000
VERSION     DADOOS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.341"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>697
                     /locus_tag="J8C37_002973"
     CDS             <1..>697
                     /locus_tag="J8C37_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAZ5306341.1"
                     /translation="WSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIVVDRLLTGF
                     DAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKNVEDATKLY
                     SKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNR
                     AYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKEGEDFSTIE
                     FYSENTTKLYDIDS"
BASE COUNT          249 a          113 c          148 g          187 t
ORIGIN      
        1 cctggtcatt agcagacatt gagcgttata acggagacat caataatcgt ttggcacgaa
       61 aaagagcgga attcaaacaa tttgggaaac aaatcgattt agcgattgta gtggatcgtc
      121 tattaacagg ttttgacgcc cctacgattc aaaccctatt tgtggatcgt aatctagagt
      181 atgcaggatt gatccaagct ttttcacgta cgaatcgtac gtatcctgat aagacaaaag
      241 gattgattgt gacgtttcgt aagccagcga caatggagaa gaatgtcgag gatgcgacaa
      301 aactgtattc aaaagcaaaa gaagaatcag gtttgattta tccaagttat caggaatcca
      361 aaaaaagatt caaacaagca tatggtaagt taaaagaggt ttcatccatg gaatccattg
      421 atgaacatac gccacttgaa atccgtatcg agtacgtcaa agcatttcaa gaattgaatc
      481 gtgcttatga atcgttagtg acttatgacg attacaatga tgacatggtg aaatctccaa
      541 ctttgaacaa tcaaattcaa cttttggaag aacaaattgg cgtgtacgag accgtgaagg
      601 gttcattaat agaggaagaa ccagaaaaag aaggagaaga tttttcaacg atcgaatttt
      661 atagtgaaaa taccactaag ttgtatgata ttgattc
//
LOCUS       DADOOS010000329          696 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.342, whole genome shotgun sequence.
ACCESSION   DADOOS010000329 DADOOS010000000
VERSION     DADOOS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.342"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>696
                     /locus_tag="J8C37_002974"
     CDS             <1..>696
                     /locus_tag="J8C37_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAZ5306342.1"
                     /translation="QGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTP
                     DVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHE
                     REKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLN
                     QWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLR
                     RDGLLKSMDFNSV"
BASE COUNT          237 a          118 c          141 g          200 t
ORIGIN      
        1 agcaaggaaa atcagcctta gaatattatc aacagtataa gaaaaacaaa tcaaactgtg
       61 gtagacgtcc gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc
      121 aaggatggac acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc
      181 gtaccattta tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag
      241 gcaagcgtaa accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta
      301 ttcatgaacg tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg
      361 acactatcgt aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa
      421 aagttatcat cacattgaaa ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa
      481 atcaatggtt tgaatctgta ccgaaaaacc tattcaaatc catcactttt gattgtggaa
      541 aggaattttc aaattggaaa cagatcagta atgtcaatga tattgccatt tatttcgctg
      601 atccaggaac gccgtctcaa agaggcctaa acgagaattc taacggattg ttacgtagag
      661 atggtttatt gaaatctatg gatttcaatt cagtag
//
LOCUS       DADOOS010000330          689 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.343, whole genome shotgun sequence.
ACCESSION   DADOOS010000330 DADOOS010000000
VERSION     DADOOS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.343"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          231 a           98 c          115 g          245 t
ORIGIN      
        1 gtaattcctg gagttttagg tattattgcg ggattgatgc ttctaataag aaaatcttaa
       61 ttaggcaatg tgtagacaac gtgaaacgat taatcaatta aaagctaaat acgttttgag
      121 aaatgataac ttgtagtcga tggaagacat gtagataaaa aaactacgtg tcttttattt
      181 attctaattt tttcgaaaaa aagacactct ctgattgttg tattaatgga aaaggggaaa
      241 gcagcaagta aatttagttt ttctattaaa tattcttcac tttttaacct tttatagtac
      301 ttagatttaa tcctatatgt ttagagagtt cattggcagc atgattttgc aaagcttctc
      361 gaattattgt tatattagaa actttactgt tcataattat gcttcttttt taactcaaag
      421 tccaaagggc tacagtggac tttgagttaa aaataaaaag ccctagttat tttgaaaaat
      481 aaaaaaagaa cagcgtaaat gttttcgaaa tgctttcgca ccaacattta cctgttccgt
      541 gggtttggca cccaatgggc cgtgaggggc tcgaacccgc gacccgctga ttaagagtca
      601 ttaatgtcaa tttttaaata aaattaatat gtctctttaa gtattaaact atcactcttt
      661 atcctatgat aaatcaatgt tttttaata
//
LOCUS       DADOOS010000331          686 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.344, whole genome shotgun sequence.
ACCESSION   DADOOS010000331 DADOOS010000000
VERSION     DADOOS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.344"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(109..>686)
                     /locus_tag="J8C37_002975"
     CDS             complement(109..>686)
                     /locus_tag="J8C37_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAZ5306343.1"
                     /translation="EAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLF
                     NREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGIT
                     RSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTL
                     AYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          194 a          158 c          127 g          207 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcat
//
LOCUS       DADOOS010000332          669 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.345, whole genome shotgun sequence.
ACCESSION   DADOOS010000332 DADOOS010000000
VERSION     DADOOS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.345"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(1..448)
                     /locus_tag="J8C37_002976"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="J8C37_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          200 a          135 c          100 g          234 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttc
//
LOCUS       DADOOS010000333          662 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.346, whole genome shotgun sequence.
ACCESSION   DADOOS010000333 DADOOS010000000
VERSION     DADOOS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.346"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>662
                     /locus_tag="J8C37_002977"
     CDS             <1..>662
                     /locus_tag="J8C37_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAZ5306344.1"
                     /translation="YLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIG
                     LVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGG
                     IYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVM
                     VVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVAS
                     AIV"
BASE COUNT          202 a           82 c          125 g          253 t
ORIGIN      
        1 tatttgattt tatcttttat atctactttt ttgaatattg caatctctgt agctttgatg
       61 cagacattat attttgatga taaatatgtt ggcagagtag tcggcggaac actgtcattt
      121 gtgttgatag gtttagtaat ttatttaaga ataatgataa agggtgctca tttttttgat
      181 aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc taatcccaca ttccttagca
      241 aatatagttt tagcacaatt cgatagaatt atgattaatt cttatttagg tcctgctgcg
      301 gggggaatat attcctacat tagtaacata ggtattattc taagcgtaat atggatgtct
      361 actaataacg cctgggttcc atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa
      421 gtgaagaaag ttagtaacta ttatttagta gtctttactt tgattacgat gattgtaatg
      481 gtagtttcga ttgatttagc aaaatttatg gctccgaaag actattattc tgggttatct
      541 ttagtggttc cgataacctt aggctatttc tttcagtttt tatatagttt accagttaat
      601 gctgaatttt atgagaaaaa aacatcgttt attgcgattg gtacagtagc aagtgctata
      661 gt
//
LOCUS       DADOOS010000334          639 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.348, whole genome shotgun sequence.
ACCESSION   DADOOS010000334 DADOOS010000000
VERSION     DADOOS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.348"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..531
                     /locus_tag="J8C37_002978"
     CDS             <1..531
                     /locus_tag="J8C37_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306345.1"
                     /translation="IQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGG
                     EPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDF
                     NDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMK
                     NILKNVKIAESLDDIL"
BASE COUNT          247 a           76 c          111 g          205 t
ORIGIN      
        1 atacaaattc cattagttac agctgggtca agaaccgcat tgcaactttc ggatattttt
       61 aaccctatga tagatattta taaagaaaaa aaatatatat tttttacgtc tgtttatgat
      121 tttgagggcg gagaacctgt cggagaatat gaattagtat gcaaaatagc agacttagta
      181 ggaaaagata atctaattgt taagacgcat cctagagatg taagaacaat ttataaagat
      241 aatggattta tcgaggataa aaattcatca ataccttggg aggcgattca attgtcaggc
      301 gattttaacg ataaaatatt tatgacaatt aattcaggaa gtgttatttc aggtagtaca
      361 atgtctgcaa aacctgtacg cacgtattat atgtataaat tgtgtaacat agaaggaaat
      421 ccgtcttgca aaaagaatgc gtatgatatt gagcgattgc ttaagcaaaa aacgatgaaa
      481 aacattttaa agaatgtgaa aatagcagaa agcttagatg atatactatg agcaaaacta
      541 aatatttaat taaaaatatg ggaatattaa caataagtaa tttttcttca aaaattttag
      601 tttttttgtt agtcccttta tatacaagtg tattatcca
//
LOCUS       DADOOS010000335          628 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.349, whole genome shotgun sequence.
ACCESSION   DADOOS010000335 DADOOS010000000
VERSION     DADOOS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.349"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..194)
                     /locus_tag="J8C37_002979"
     CDS             complement(<1..194)
                     /locus_tag="J8C37_002979"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5306346.1"
                     /translation="METRDYIITHQKLIESGLSHFEKEGFQQTKIRQLCLTAGVTTGA
                     FYKHFTSKEALFEEIIQPHI"
     gene            complement(486..>628)
                     /locus_tag="J8C37_002980"
     CDS             complement(486..>628)
                     /locus_tag="J8C37_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAZ5306347.1"
                     /translation="AKVLSVSPASVSEMLAKLRYEDYLDIGFQLTDKGKKFIDDYKKG
                     FK"
BASE COUNT          209 a          121 c           77 g          221 t
ORIGIN      
        1 ttaatatgcg gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa
       61 aaagcacccg tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat
      121 ccttcttttt caaaatggct tagtccactt tctattaact tttggtgagt tataatataa
      181 tctctggttt ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca
      241 ggacttgaag tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa
      301 tgagaaagag cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct
      361 aaatcattca ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat
      421 taggataagt cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa
      481 gtcttttact taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat
      541 tgaaatccta tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat
      601 gctggactaa cacttagaac ttttgcta
//
LOCUS       DADOOS010000336          606 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.350, whole genome shotgun sequence.
ACCESSION   DADOOS010000336 DADOOS010000000
VERSION     DADOOS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.350"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..553
                     /locus_tag="J8C37_002981"
     CDS             <1..553
                     /locus_tag="J8C37_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306348.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRL
                     SEEKILLPENWAELRKLVEYGII"
BASE COUNT          228 a           83 c           93 g          202 t
ORIGIN      
        1 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
       61 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      121 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      181 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      241 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      301 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      361 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcattatc
      421 tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct
      481 atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta
      541 tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta
      601 taggga
//
LOCUS       DADOOS010000337          604 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.351, whole genome shotgun sequence.
ACCESSION   DADOOS010000337 DADOOS010000000
VERSION     DADOOS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.351"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            179..577
                     /gene="spxA"
                     /locus_tag="J8C37_002982"
     CDS             179..577
                     /gene="spxA"
                     /locus_tag="J8C37_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAZ5306349.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          211 a           94 c          118 g          181 t
ORIGIN      
        1 aagcgagaat gctggtttta ctgtatatta accttagata actttcattt ttttaaaaaa
       61 attatgaaaa aaaggtagct atttaaaagt tgattatgta taatagaaac agatgggaga
      121 ttattatgat cttctgttac aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt
      181 gttaacatta tatacttccc caagttgtac ttcctgcaga aaggctcgtg cgtggttgca
      241 agaacatcag attccatttg ttgaacgtaa tattttttca gaaccattga acagttctga
      301 attaaaagcg attttacaaa tgacagaaga cggaacagaa gaaatcatct cgacacgttc
      361 caaagtattc caaaaactga acatggattt agatgatctg ccgttacaag aattattgga
      421 acttgtgcaa aacaatcctg gattgcttcg ccgtccaatc atgattgatg ataaacgtct
      481 gcaagttggt tttaatgaag atgaaattcg tcggttccta ccgcgcgatg tacgacaatt
      541 agaattacgt caagcacaat tgatggctgg attatagaag gataatacga aacttggaaa
      601 agac
//
LOCUS       DADOOS010000338          597 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.352, whole genome shotgun sequence.
ACCESSION   DADOOS010000338 DADOOS010000000
VERSION     DADOOS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.352"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..>597)
                     /locus_tag="J8C37_002983"
     CDS             complement(<1..>597)
                     /locus_tag="J8C37_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAZ5306350.1"
                     /translation="NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYP
                     RINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDY"
BASE COUNT          197 a           85 c           72 g          243 t
ORIGIN      
        1 agtaatccga tttaactttt cttacaaata atttttcact ttcttttatt tgatctatat
       61 ccctttcatc aaaccatttg ttcatggaag gatcaataat atgaatattt gctaatttcg
      121 aagtttgtaa agtagtttct tcaatatttt tcaattcaag tggtaaatct ggtcctccaa
      181 taacattttt atttttgaac gaggaattaa aaaaaagtgt atgaaaaaat ttttcatctg
      241 gggcaaacat acctatttta aaattcaggt agtcttgttg attttgttca tacatttgta
      301 ttaattctgt taggcattct ttcgacaaag cccaccattg agaaccatga tacaaatcaa
      361 atttatggtg tctaattttt gggggaagtt taatcatcct cagacaacga ttaattatag
      421 ctctaaaaat tttaaatgca gttgtgttat tcctattaat tctaggataa tcatgttttt
      481 gatatacatc tatatgttta taataaaatt ctttatgttt aatttgatct agatctattc
      541 cacgaataaa ctccactgaa ctattctttt taaaataatt ataaatatac tcattat
//
LOCUS       DADOOS010000339          538 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.356, whole genome shotgun sequence.
ACCESSION   DADOOS010000339 DADOOS010000000
VERSION     DADOOS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.356"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(18..371)
                     /gene="tnpB"
                     /locus_tag="J8C37_002984"
     CDS             complement(18..371)
                     /gene="tnpB"
                     /locus_tag="J8C37_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAZ5306351.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          155 a          120 c           92 g          171 t
ORIGIN      
        1 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
       61 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      121 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      181 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      241 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      301 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      361 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      421 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      481 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DADOOS010000340          522 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.358, whole genome shotgun sequence.
ACCESSION   DADOOS010000340 DADOOS010000000
VERSION     DADOOS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.358"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..123)
                     /locus_tag="J8C37_002985"
     CDS             complement(<1..123)
                     /locus_tag="J8C37_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAZ5306352.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVL"
     gene            complement(253..>522)
                     /locus_tag="J8C37_002986"
     CDS             complement(253..>522)
                     /locus_tag="J8C37_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAZ5306353.1"
                     /translation="SAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEE
                     IHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          189 a           90 c           70 g          173 t
ORIGIN      
        1 caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa
       61 aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga
      121 cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc
      181 gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca
      241 agcttttttt aattaactca gtaattcaat ccacccagga acagaaaatt gatacaatct
      301 tctggttaat agcatagtct tttcttttga atagctatgc ttcaatatat catagaatgt
      361 agcgtagaat gagtaaatgt agaagtggat ttcttcttca ttaaagacta aattttgatt
      421 gatcattccg atagtcttta acttatctag acaacagact gtctttttcg tcacaaatga
      481 tgtaattttt tcaacaatat cttgatattt ggttccagct ga
//
LOCUS       DADOOS010000341          517 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.359, whole genome shotgun sequence.
ACCESSION   DADOOS010000341 DADOOS010000000
VERSION     DADOOS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.359"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            27..>517
                     /locus_tag="J8C37_002987"
     CDS             27..>517
                     /locus_tag="J8C37_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAZ5306354.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDA"
BASE COUNT          189 a           95 c           86 g          147 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgc
//
LOCUS       DADOOS010000342          514 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.360, whole genome shotgun sequence.
ACCESSION   DADOOS010000342 DADOOS010000000
VERSION     DADOOS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.360"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(107..>514)
                     /locus_tag="J8C37_002988"
     CDS             complement(107..>514)
                     /locus_tag="J8C37_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAZ5306355.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DADOOS010000343          494 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.361, whole genome shotgun sequence.
ACCESSION   DADOOS010000343 DADOOS010000000
VERSION     DADOOS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.361"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            38..127
                     /locus_tag="J8C37_002989"
     tRNA            38..127
                     /locus_tag="J8C37_002989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="J8C37_002990"
     tRNA            142..213
                     /locus_tag="J8C37_002990"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="J8C37_002991"
     tRNA            229..301
                     /locus_tag="J8C37_002991"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="J8C37_002992"
     tRNA            311..383
                     /locus_tag="J8C37_002992"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
     gene            419..494
                     /locus_tag="J8C37_002993"
     tRNA            419..494
                     /locus_tag="J8C37_002993"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Phe,seq:gaa)
BASE COUNT          111 a          114 c          134 g          135 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtcccgcgcc acca
//
LOCUS       DADOOS010000344          485 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.362, whole genome shotgun sequence.
ACCESSION   DADOOS010000344 DADOOS010000000
VERSION     DADOOS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.362"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(62..259)
                     /locus_tag="J8C37_002994"
     CDS             complement(62..259)
                     /locus_tag="J8C37_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5306356.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DADOOS010000345          460 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.363, whole genome shotgun sequence.
ACCESSION   DADOOS010000345 DADOOS010000000
VERSION     DADOOS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.363"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(20..91)
                     /locus_tag="J8C37_002995"
     tRNA            complement(20..91)
                     /locus_tag="J8C37_002995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="J8C37_002996"
     tRNA            complement(113..200)
                     /locus_tag="J8C37_002996"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="J8C37_002997"
     tRNA            complement(225..298)
                     /locus_tag="J8C37_002997"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="J8C37_002998"
     tRNA            complement(312..382)
                     /locus_tag="J8C37_002998"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="J8C37_002999"
     tRNA            complement(384..459)
                     /locus_tag="J8C37_002999"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DADOOS010000346          455 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.364, whole genome shotgun sequence.
ACCESSION   DADOOS010000346 DADOOS010000000
VERSION     DADOOS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.364"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>455
                     /locus_tag="J8C37_003000"
     CDS             <1..>455
                     /locus_tag="J8C37_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAZ5306357.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV"
BASE COUNT          150 a           95 c           90 g          120 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct
      421 ctaaaggagg cgtatccctt ttttcatgaa ttagt
//
LOCUS       DADOOS010000347          454 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.365, whole genome shotgun sequence.
ACCESSION   DADOOS010000347 DADOOS010000000
VERSION     DADOOS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.365"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          155 a           73 c           69 g          157 t
ORIGIN      
        1 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaatt
       61 gtgagataat aataaagaag agtttaaagc gcaccccaaa ccacttcccc ataagtgtgt
      121 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
      181 agtgagaaaa aaggcttaca attggagtgg tagttaatta gtgacttatt tttgatttta
      241 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
      301 aaaatatctc ctttcacaag tatggtgata aaattcgttc cgggctacct ttttaggtag
      361 cctactttaa tctttatacc tttctggatc aacgaaagta tactttatat agtcataacg
      421 ccgatgatcg ctacgtgcgt ccggcacgtc agtc
//
LOCUS       DADOOS010000348          453 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.366, whole genome shotgun sequence.
ACCESSION   DADOOS010000348 DADOOS010000000
VERSION     DADOOS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.366"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..>453)
                     /locus_tag="J8C37_003001"
     CDS             complement(<1..>453)
                     /locus_tag="J8C37_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306358.1"
                     /translation="GLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLV
                     FIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLY
                     LSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTK"
BASE COUNT          177 a           93 c           46 g          137 t
ORIGIN      
        1 tttttagtta gtcttatcgg tttcacaaaa aataaccaaa acatccatat aataaaaact
       61 atacatccaa cttctatata aatagttaat atatcactgt gtaggcttgc tgcaattctt
      121 ccaccattta acctaaagtg cgataaataa agattaaaaa agtattttga aaaccatgtc
      181 catcctgttc ctacatatcc taaaccaagt tcataaaatt tagatgaata tagataaaat
      241 gaaagcctcg caccgtcatc tgtaatgccc aaatttgaca taagcaatgc caaaattcct
      301 gtatctataa tgtaaataaa cactaacgaa ataacagttg cacaaatagt acacactaat
      361 gcacggattt gaatcttttt tccccacttt aataaaacat aatacactag aattgcacca
      421 cttaaagcta acagttcaat tctcttcaat ccc
//
LOCUS       DADOOS010000349          450 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.367, whole genome shotgun sequence.
ACCESSION   DADOOS010000349 DADOOS010000000
VERSION     DADOOS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.367"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
BASE COUNT          164 a           63 c           54 g          169 t
ORIGIN      
        1 ttgtaattcc taattttgct ccacttttag ataattttta ggtctattta actcatcaga
       61 cattctatgc tcatttttct ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat
      121 ttgaaaattc ttcttttaga gtacttttat tacgatacta gtatgatata tatctatagc
      181 aaacagttaa aatcttccta aaataagcga atagcattcc taaaacctat aaaattttta
      241 gatgaagcga caagtggaca attaataaac tacttgaaca tgaaattatg gatcaccttt
      301 caaatttaga aaatggaatt cttatactta ttgcgcaaca tttattgatt gcaaaattat
      361 gtgacaaaaa aaactagtag aagaaagaat aatggcttga taataaagta ctacctatta
      421 aaggtagtac tttatttaat ttagtataaa
//
LOCUS       DADOOS010000350          437 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.369, whole genome shotgun sequence.
ACCESSION   DADOOS010000350 DADOOS010000000
VERSION     DADOOS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.369"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>437
                     /locus_tag="J8C37_003002"
     CDS             <1..>437
                     /locus_tag="J8C37_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306359.1"
                     /translation="SDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESKSFDDNI
                     GYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNNKN
                     VQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSY"
BASE COUNT          169 a           47 c           76 g          145 t
ORIGIN      
        1 ttccgatgag tatgttgaaa ttattatttc tgatcattct aaaaacacta aagaaattgc
       61 gaaaaaaata gaagaaaaaa aagtgtttga tggctgtcat tatttagaaa gcaaatcttt
      121 tgatgataat attgggtatt ttcaaaagat taaggactgt cttcagattt cttgtggtaa
      181 atcaaatcga tatgcctatt atttgaaaaa ctgtaaaagt gaatattttg atgaatttat
      241 ttgtttttca atgggagagg tttggttgga aggaatatat gcttgcttat atcgaaataa
      301 taaaaatgta caggtatcaa ttttagaaga aggtatttta agctatggag ttgacattat
      361 tccaacagca agaacaaaag ctatcgcagt actcaattac ttaaaaaaaa ggactccact
      421 atataacaaa agctata
//
LOCUS       DADOOS010000351          408 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.370, whole genome shotgun sequence.
ACCESSION   DADOOS010000351 DADOOS010000000
VERSION     DADOOS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.370"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..390
                     /locus_tag="J8C37_003003"
     CDS             <1..390
                     /locus_tag="J8C37_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAZ5306360.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           73 c           64 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggag
//
LOCUS       DADOOS010000352          375 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.denovo.372, whole genome shotgun sequence.
ACCESSION   DADOOS010000352 DADOOS010000000
VERSION     DADOOS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.denovo.372"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="J8C37_003004"
     CDS             complement(<1..132)
                     /locus_tag="J8C37_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAZ5306361.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DADOOS010000353         6732 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.133, whole genome shotgun sequence.
ACCESSION   DADOOS010000353 DADOOS010000000
VERSION     DADOOS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.133"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..188
                     /locus_tag="J8C37_003005"
     CDS             <1..188
                     /locus_tag="J8C37_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAZ5306362.1"
                     /translation="GDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMEDI
                     QLEGYDPHPAIKAPIAV"
     gene            208..735
                     /locus_tag="J8C37_003006"
     CDS             208..735
                     /locus_tag="J8C37_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAZ5306363.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(798..1451)
                     /locus_tag="J8C37_003007"
     CDS             complement(798..1451)
                     /locus_tag="J8C37_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAZ5306364.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1583..2425
                     /locus_tag="J8C37_003008"
     CDS             1583..2425
                     /locus_tag="J8C37_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAZ5306365.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            2583..3479
                     /locus_tag="J8C37_003009"
     CDS             2583..3479
                     /locus_tag="J8C37_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAZ5306366.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            3482..4195
                     /locus_tag="J8C37_003010"
     CDS             3482..4195
                     /locus_tag="J8C37_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAZ5306367.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            4215..4733
                     /gene="msrA"
                     /locus_tag="J8C37_003011"
     CDS             4215..4733
                     /gene="msrA"
                     /locus_tag="J8C37_003011"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAZ5306368.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            4730..4957
                     /locus_tag="J8C37_003012"
     CDS             4730..4957
                     /locus_tag="J8C37_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAZ5306369.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            5109..5660
                     /gene="lepB"
                     /locus_tag="J8C37_003013"
     CDS             5109..5660
                     /gene="lepB"
                     /locus_tag="J8C37_003013"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAZ5306370.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(5743..6285)
                     /locus_tag="J8C37_003014"
     CDS             complement(5743..6285)
                     /locus_tag="J8C37_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAZ5306371.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(6522..>6732)
                     /locus_tag="J8C37_003015"
     CDS             complement(6522..>6732)
                     /locus_tag="J8C37_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAZ5306372.1"
                     /translation="EIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGM
                     PEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         2427 a         1190 c         1277 g         1838 t
ORIGIN      
        1 taggagatgc tcatctttat atcaatcata tcgaacaaat gaaagaacaa ttgagccgag
       61 aaatacgcgc atttccgact ttagttttga atcaggaaaa gcagtcagta tttgattttg
      121 agatggaaga tatccaatta gaaggatatg acccacatcc ggcgatcaaa gctcctatcg
      181 ctgtttagaa tcagaaagga tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac
      241 ggattgattg gaaaagaaga ccgtcttcct tggcgtttgc caaatgatct gaaattcttc
      301 aaacaaatga cagaagcaaa tacactagtg atgggacgaa aaacattcga agggatgggt
      361 agccgtccgt tgccgaatcg tcagacgatt gttttgacga gagattcttc ctataaagca
      421 gaaggcgtgc atgtcatgca tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac
      481 ggtatctttt tcatcgctgg agggtcagct gtttatcaag aatttctgcc attttgtacg
      541 atattatacc gtacagtcat ccatcatgta ttcgatggag atgcttattt tccacctgtt
      601 gactggtctg attggtcact gatcaatact agtcaaggag aaatggatga aaaaaatcgt
      661 tatccgcatc aatttgaaac gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc
      721 gaattgacga tataaaaaag ctatggatag actagctgtt atttagagct gtcccatcca
      781 tagctttatt ttttttatta agtaaagaat aaaacagaga agtacataaa acctgcacct
      841 aacataacaa acaaatgcca aacaacatgc atatagcgga tagaattcaa gctataaaag
      901 aatgcgccta tcgtgtaaga aagcccgcct gctaccatca aaataacacc agtcattcca
      961 agagagtgat aaagaggaac agcagccacg acacagagcc accccataaa aatatagatg
     1021 atcgtagata cttttgatac tctctcccgt ttatgcaacg tcaacgcttt atatataatg
     1081 ccaagaatcg caaacaacca gattaataca aataaagtcc aacctagcca tcctcggata
     1141 ctcagcaaac aaaaaggagt ataacttcct gcaatcaaaa gaaaaatcga actatgatcg
     1201 aatacttgga acaccttttt tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt
     1261 aaaaataaaa gaatcatcat cgatccatag atagcataag agacaatatg gagggaggaa
     1321 tgcaagtggg caccttttac taaaagaacc actagtcccg cgatactcaa tccagtccca
     1381 attccatggg tgactgcatt aaacacttca tttaatattt catacgttcg attaaatttc
     1441 gttttttcca tgaaaacggc tcctttacta ggtgtaatta atgaaaaatc attatttgtc
     1501 tgttatacta aaaatagaaa ttgtcttaca tgtagtttaa catgaataag gcatcttgtt
     1561 aaagaaagag agagagaaaa taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac
     1621 gatggaaaaa aatgtacgcg atgctttagg cattcgtgtc atgccgctat ccatcatgat
     1681 cgacggaaca gtatatcccg atgatgacca actagacggc gaaagattta tggaaatgat
     1741 ggcgaaagca aaaaatctgc ccaaaacaag ccaaccacca attggagaat tcatctcgat
     1801 gtatgatgag ctaggagaag acggcagtga agtgatttcg atccatatga caaaaggctt
     1861 gagtggaaca gtagaagcag caagacaagc cagtaatcta acaaaaacga aagtaacggt
     1921 tatcgacagt gataccactg accaaggatt atcttttcag gtaattcgag cagctgaatt
     1981 agcaaaagaa ggtaaaacag tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa
     2041 tacaaaatta tacatcggaa tatccacact agataacttg gtaaaaggtg gaagaatcag
     2101 ccgaacaact ggtttgctgt ctagtgtttt gaacatacgc gtggtgatga atttcgccca
     2161 ttctgaatta atccctgtaa ctaaggggcg aggaaagaaa acgttcgata aatggtttaa
     2221 taatttgaaa aatgaactga aagaattgcc aaatgtgcga caaatcggta tttcacatgc
     2281 ggatgcaaaa gaattggcgc aagaattcaa agagggtttg caagaattat tcccggacat
     2341 gcatatccct gtactgcata cgaatccggt tatcgctacg catactggta aagacgcgtt
     2401 tgcaatcatg tattatgtag attagtcgaa aagaggataa gccctcagcc tgaaaggctt
     2461 gcagcttatt ctctttttct ttataaaatt aggaaaataa ttgttttctt ttttcataaa
     2521 gactaccttt ttgataaaat ttttgataac attagaacca atcgaataca agaggtgaaa
     2581 aaatgaaatt aacgaaacga catttgatcc tcgttttgtt cccattatta attgcgatca
     2641 tcatctatgc aggtttagct gcttctatac cgaaagctgg cccactaatg aaaccagtaa
     2701 ctactgtgag agaagatcca aataaaaaag aaaaacttca ctacatagct atcggagatt
     2761 cgctgactga aggagtagga gatcaaacca agcaaggagg attcgttccc cttgtcgcaa
     2821 atgatctaaa agaccgttat aatttgaccg cggttgaaac agaaaactat ggtgtaaatg
     2881 gtgaacgaag tgaccagatc ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata
     2941 tcaaaacagc tgattttatc acactgactg ttggcggtaa tgatttgatg aaagttatcc
     3001 aaaataattt attcgggtta acggtcaatt cctttaaaaa gccactgaaa aactatcagg
     3061 aaaatgtcac agaactattg gaagaatttc gatccctcaa taaaaccgca cctatttaca
     3121 ttttaggcat ctacaatcca ttttatttga actttcctga gatcactgac atgcagacaa
     3181 ttgttaacaa ttggaacaaa ggaacagaaa cggtgacaaa aaaaatgccg aactgctact
     3241 ttatcccaat caatgatttg ctttatcaag gattgaatca ggaagtaggt gtcacagaag
     3301 aaggaacaga tcaaacaact agcacgacag aaaatctaaa tggcatcaaa aataatgtgc
     3361 tctatgaaga agatcatttc catccaaata atttaggtta tcaattgatg gcaaatgcag
     3421 tgagagatca gctgatcgag acacaaaacc aatggttaga gaaggaggat gatcaataaa
     3481 catgaaaaca agatctgacg tatcttcaaa caaaacaaag aaaacaaatt actggaaatt
     3541 cgccttcctt atccttcttg gggtagtact tggttcgacc gtatttttag gcagccgtat
     3601 ttttgctaat cgggaaccag aactcccaga aataccagct cttacggaaa gacaaggaga
     3661 tcctgtctta acgatcaatt caaataaaga aaaagtcaat caaatcatca gctttttcct
     3721 tagtgagtat caaaaagata gtgatattga atataaattt tatttggaaa atgaagcact
     3781 actcaatggt actttcgaag tactaggttt tcctatcgat ttctatttat attttgatcc
     3841 gtatgtgatg gaaaacggaa atgtccaact aaaagctaga agtctttcta tcggaacatt
     3901 gagtttgcca atcaaagatg tgatgaatat gatcaagaga aactataaat taccagattg
     3961 gatcgaagtc aatacagatg atctgactat catgcttagg ttggatcaat tccgaatgca
     4021 aaacgggatg tatattaaag cagataaaat cgacttagta aatgatgaca ttcggtttag
     4081 tctatatttg ccaaaagata cctctgatgc ttcaaacaac tcaaatacca acaaaactag
     4141 caccagcagt actgaaagca agacaaaaga aaataaaaaa tcatcggatg agtagtatta
     4201 gagggggaat cataatggaa cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc
     4261 agcctttcga cacgcttcct ggtatccata cagtgatgtc aggatatact ggagggcatg
     4321 taccagatcc gacttatgaa caagtaaaaa gccaaacgac tggacataca gaagcagtgg
     4381 agattatcta tgatccaaca ttgatttcat atgaacaatt agtagaactt tactggcagc
     4441 aaactgatcc aactgacgct ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg
     4501 tgatctttta tttgacagaa gaacagcgga tcattgctga aaaaagtaag aaacagttag
     4561 ctgaaagtgg acgtttcgaa cagccaatcg ttacaaaaat cgaaccagcc caaccatttt
     4621 atctagcaga agaagaccat caagagtatt ataaaaagca tcctgaaaat tttaaaagaa
     4681 accatgcacg ccgggctgcc tttatcgaag aaaattggag gaacgaaggg tgaatcgaag
     4741 cttttttcat tatttaatga cactgcgagg cggtggcaca catgatgctt tatctgtttt
     4801 cgctacagaa gccggtaaag acatccaatt tcctaagcac agctctagct acgaagaaat
     4861 ttctaattat ctggaactga atgtcgacta tttgccgtcc atggatatct ttgacgaagc
     4921 atgggaaaaa tatttagaaa ataatcatga catataagag atagttttgt actttatgat
     4981 caaaaaagtt ttttgtccaa tagcctaatt gaaagaatta tttttatttc agctatctag
     5041 tcgcattttt ttgatatttt tcttataata aagaaagaat gaaaaaaaga gggatggatt
     5101 gatagagatt gacaaaaaaa caacgctata ttaatcgctt ctggcttgta ttcaaatact
     5161 tacttgtttc tgtattcctt gcgttcatgt tgcggggatt tttattcatc ccggtaccag
     5221 tggaaggcaa ttcaatggaa aacgtgttga agcaaggtga tatggttgtg atggaaaaat
     5281 tttcggagat cagacgattt gatattgttg tttttcaatt agcagatggc acgatttata
     5341 tcaaacgagt aatcggtctt ccaggagaaa atgtcagcta ccaaaatgat caattgaaga
     5401 tcaatgggaa agtagttaag gaaccttatc taactaaaaa cctgaaatca gatcatgcaa
     5461 atgcatccta tacgacggac tttacactac aagagctaac gggacaatcc aagcttccgg
     5521 aagataatta ttttgtactt ggagataatc gaagagtttc aaaagatagt cgttcgtttg
     5581 gcacgatcaa taaaactgat attctaggta aagcaagatt tgtctattat ccacttgatg
     5641 aaataaaatg gatccagtga gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt
     5701 attgtttaat ccaaaatcag tttactacct tagtacgtaa aatcaaacag ctcgtttaaa
     5761 cacttttcta gcctctaaag ttttgataaa tgaataaaca accattattt gaatagggat
     5821 ttggatcata ttcctgatga atctacctag aaaaattact ttgaaatctg caccaaacaa
     5881 aacagacatc agccaagaat tgataaaaag gctgactggg atcataatca ataattgggc
     5941 agcgatgacg gaaggcaggt ttatcttttt atgatataaa aataagccat taataaatcc
     6001 accaaggaaa gccgtgataa gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa
     6061 atttaaagtg ttcgacaaca tttcaagcaa accagcccag atcggtccat atagataacc
     6121 cataagcgcc atacttaaaa atccgaatgt gatttgaaaa taagcaggac caatcgctat
     6181 cttgtataag atgacatcaa gggctaataa aagagcagag gtcgacaaca atcttgcatt
     6241 catttcgggc aatctaaaaa cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt
     6301 gtcagttaat aataaaagga taccataaaa tcaccaatgt gtcatgacac attggtgatt
     6361 tacatacact ggcatctaac aattaaaaaa agggtgagaa gactgctttt ataaactgta
     6421 aaaaagagat tgtacgaata aaaaaaatag aggcggagaa tacttatcgt atcatccgcc
     6481 tttgtttttt tgttataaaa aacgtcagat tagttatctg cttacatctt catccatacg
     6541 ggttctaatg tatttccgtc tagatcttgg acttctaatg aaaacatttc ttcttcaggc
     6601 attcccatat ctacttcgtg gaaagtacca ccattttctt ttgcaatttg agcaaatttt
     6661 tttacttcat ccgcgttttc aacactaaac gaaatcaaag caccacttgt ttttttatta
     6721 tcagctattt ct
//
LOCUS       DADOOS010000354         5986 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.150, whole genome shotgun sequence.
ACCESSION   DADOOS010000354 DADOOS010000000
VERSION     DADOOS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.150"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            complement(41..1027)
                     /locus_tag="J8C37_003016"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="J8C37_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="J8C37_003017"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="J8C37_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAZ5306373.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="J8C37_003018"
     CDS             1852..3006
                     /locus_tag="J8C37_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAZ5306374.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="J8C37_003019"
     CDS             3221..4189
                     /locus_tag="J8C37_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAZ5306375.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="J8C37_003020"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="J8C37_003020"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAZ5306376.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="J8C37_003021"
     CDS             5219..5827
                     /locus_tag="J8C37_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAZ5306377.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1348 g         1666 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     5881 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagatgta
     5941 aggcagaggg ttctgttgca aagttttaaa taaagaataa aatccc
//
LOCUS       DADOOS010000355         5480 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.158, whole genome shotgun sequence.
ACCESSION   DADOOS010000355 DADOOS010000000
VERSION     DADOOS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.158"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            495..959
                     /locus_tag="J8C37_003022"
     CDS             495..959
                     /locus_tag="J8C37_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5306378.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            943..1572
                     /locus_tag="J8C37_003023"
     CDS             943..1572
                     /locus_tag="J8C37_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ5306379.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1926..3404
                     /gene="msr"
                     /locus_tag="J8C37_003024"
     CDS             1926..3404
                     /gene="msr"
                     /locus_tag="J8C37_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAZ5306380.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3451..3897
                     /locus_tag="J8C37_003025"
     CDS             3451..3897
                     /locus_tag="J8C37_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAZ5306381.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4096..4611)
                     /locus_tag="J8C37_003026"
     CDS             complement(4096..4611)
                     /locus_tag="J8C37_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ5306382.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4728..>5480
                     /locus_tag="J8C37_003027"
     CDS             4728..>5480
                     /locus_tag="J8C37_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5306383.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELE"
BASE COUNT         1739 a          910 c         1146 g         1685 t
ORIGIN      
        1 gttattaaaa agaggtagtg aaaaagctgc cctgcatcaa gtagtaagaa cagccaaaca
       61 aaaacagatt ttcatgtttt ttgtttggtt gaacttatta ccgcagatgc aagtttttga
      121 acaccgttta ctcaaaaaag gttgtgacaa catcttgtca taactttttt attactgttt
      181 aagcatactg atccaatttc taatattcga cgggattctt gcaaaaggcg atttcggaaa
      241 tttttggcgg tgtaaaatta tctactgcag aagagctatc ttctatagtt tgataagaat
      301 caataatctt ggttttagcg ttgtgctttc tttatcattt tttccgttca ttctacaaaa
      361 aatttctttt ctctatactt ttctattgag agcgtttttt ttattacaca gctgattctt
      421 tgactttttt ctttttttat tctattattg acaagtcaat cgttgacgag tcaattatta
      481 gaaaggagga aattatgtct ttagaagaga ttagtcacct tttatatcaa atcaaattag
      541 ccgatcaaaa aatcgtccag atctttgaag aaaatatcgg tataagtctt actagatacg
      601 agatcatgat gagactgaaa gaagaaaatc cattgctaca aagcagtttg caagaaaagt
      661 tgcagattga tcaaggagca gttactcgtc atctgaaggt attggaagca aatggctatg
      721 tttcgcgtca gagaaatcct cagaacaacc gggaagtgta tgtagcgcta acagaaaaag
      781 cccagaagaa aatcaagcat tgtgaaatgg accataaaga tacaggttcc gtgctatccc
      841 cggctttcag cgatgtagaa atgaaacaat ttctaacact tttagataaa ttgaataccc
      901 aactttgcaa tataacaaca gaaacgaaag aggttaataa taatggaaca aaaaaatgat
      961 ttttcaacaa ttatgactgg tagaagatcc gttcgcgtat atgatgaaac atacaaaatc
     1021 ccacacgaag aaatgctgga aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat
     1081 atgaaaccat ggcgctttgt ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct
     1141 ttgatccgtt tcaatacgag acaaaatgat acttcttctg caatggtcat gatttttggc
     1201 gatatgcaat gttacgaata tggcgaagaa atctacaacc aagcttacga atcagggaag
     1261 atgccaaaag aagtcagaga tcaacaatta gctgcgatca ttccttatta caagagtttt
     1321 tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc
     1381 atgttagttg cgcgtgatca tggctatgaa acaaatccaa ttggtggatt cgaagcagat
     1441 cagttggcag aggcatttgg tttggataaa gaccgttatg ttccagttat tattttatca
     1501 ataggaaaag cggtagaaga aggttacgag tctgttcgtt tagcaccaga aaagatcact
     1561 atgttcgaat aaaaggtttt cagttactat tcagtagcta ttgctttttt tgaatcgtgt
     1621 gtggtaacct aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg
     1681 aacttttgaa taacaactaa ataaattcaa aagacatgta taaacaacaa tttcatggat
     1741 ggagtatgtc cattttttcg aacgacagta gaataggtga aagctatgac agatttggtg
     1801 atttagtcat agttatttcg ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac
     1861 agatttttct gctctttttt agtatcgata attgttgttt tacatgaaat aggagtgtaa
     1921 aacatatgga aaatttagca gtaaatataa caaatctaca agtcagcttt ggcaaccagc
     1981 tagaactatc tattgattct cttcgtgtct atcagcaaga tcgtataggg atcataggag
     2041 aaaatggtgt cggtaaatcg actttgctca aactaatagc cggtgaactt tttcccgatc
     2101 atgggaaaat ccaaacagag atcaccttca actacctgcc tcaattaacc tatctcgctg
     2161 aagcaaagga cctaaatttg gaattagcca gtcatttcca gttaagactg gaagaaactt
     2221 cagagcggaa atggagcgga ggggaagaac gaaagatcga gttgatacgt cttctttctt
     2281 cttatgaaca agggatgctt ctagacgagc caacaaccca tctagataga aaaagtattg
     2341 atcgactgat tgaagagctt cgttattatt atggtacgct ggtttttgtt agtcatgatc
     2401 gctattttct agatgagcta gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag
     2461 agttttcggg gaattatagt gcctatctca ctcaaaagga attggagaaa aagactcagc
     2521 tacgagaagc agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa
     2581 agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca
     2641 gactggatcg gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga
     2701 atgcgaaaac attagagaga agactccaaa aaataggaga aacaaccaag ccgcagcaga
     2761 tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact ccacagccgc tatcccatca
     2821 tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt actggtaaat ggtgattttc
     2881 agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt aagacaacct
     2941 tattagaaca tatccgcaaa caaggagaag gaatccttct ctctccgaaa gtaagctttc
     3001 aagtatatca gcaaaagggt tatcaaatga catctgaaga atccatcatt cgttttgtca
     3061 tgagacaaac agagttttca gaatcgcttg tccgtagttt gctgaatcac ttagggtttg
     3121 ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg gggagaagcg acccgtctga
     3181 cgattgcttt gctttttact aagccaagta atgtgttgct gttagatgaa ccgactaatt
     3241 ttattgatat ggcaacgatc gaagctttag agaagctaat gcaaatatat ccgggaacga
     3301 ttttgtttac ttctcatgat tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa
     3361 taaaagggca gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc tacgatgatt
     3421 ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa ataaaaaaga aggaataatt
     3481 tttacaacaa tgatgtgttt tttgatggtc ttggggatga gcgcgtataa tttatggctg
     3541 catcaagatc ttcatttatc aagtttgttg attggtctag tgcctggatt cgtggtggct
     3601 ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt taaactacca
     3661 gtaaatccag aaagcaaggt ccaaatgatc ttaactattt catgcttgat ggttttagga
     3721 atggtgacat gtatgtcttt attcggtgtg atcatggaac aaggaatacc tgatcaatta
     3781 ttctctgctt atatgaatgc ttggaaaatg aatgtgattg ccgctttacc gttgcagttg
     3841 atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg
     3901 aacactgtta tttgaaaaaa ggccttggca tgctttttgt catagtctct tgaatgcagt
     3961 tctagaaaaa tagtgatccg aacgataggt tttggcctgt cgttcggatc gctttttctt
     4021 ttccaactgt attgctcttt tattgaagat aatttgctct tcaatagata tttctagaaa
     4081 ggttctgttt atcccttact caaaaaactg ctgcatttgt tctcttactt gctgacacca
     4141 ttggagttcc tgctgacaaa gcataattct gcgctggatc acaacataac gtccaaattc
     4201 ttgtttcttg tctagcgaga ggtcattcaa ttttttttga tttttttgca tgattttgtt
     4261 cagctgttgt tctctagtga atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa
     4321 agcatagatt tttgctagaa attcatcttt ttgcgtagga gcgggagttt cctctttgat
     4381 ccattcctct acaagagcac gtccttcatc agtcaagtgg tagatctttt tttgagagtg
     4441 ctcgtctttg tccaaaacgg tcacataacc ctgctggtct aacttggcaa gcgttgtata
     4501 gatctgactg tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt catacccaga
     4561 acgtggtttg gataaaagca cgcataataa tatataggct aaagtattca taaaaatcct
     4621 acctctcgtc ttattttctt tattatatca cagtaaagtt tccacgtttt acatgtgcat
     4681 attgacatgt aaaataaatc atgtatgatg ctaatagaga gaaagggatg gaaatattgg
     4741 agaatcaaat agatatacaa gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc
     4801 tagttgtagc agctacctgt atgatcgctt ttatgggagt gggtctagta gacccaatct
     4861 taaaaacgat tgctgcacag ttaaacgcta cgccagctga aacgaccctg ttatttacta
     4921 gttacatgtt agtcactggt gtggtgatgc tatttacagg tttcttatct agtcggatcg
     4981 gtttgaaaaa aacattgatg acaggattgt ttatcatcgt tgtattcgct ggtcttggcg
     5041 gattatctgg gaatatcgga atgctgattg ggttacgtgc cggctggggt gtcgggaatg
     5101 ctttatttgt ctctactgct ttagcaacga tcgtcagtat tttagttggt aacacagaaa
     5161 aagcaatcat gatgtatgaa gcagcacttg gctgcggaat ggctgttggc cctctagtcg
     5221 gtggggtctt gggcagtggt tcatggcgtt atccattttt tggggtagca tttttgatgt
     5281 tttgcggttt tatagcccta gcggtacttt tacctgaaac tgagaaacca aagagaaaag
     5341 taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca gttggactaa
     5401 tcgcattgtt atataatttc ggattcttta ctttacttgc gtatgcgcca tttttattga
     5461 ttggctattc tgaattagaa
//
LOCUS       DADOOS010000356         5480 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.159, whole genome shotgun sequence.
ACCESSION   DADOOS010000356 DADOOS010000000
VERSION     DADOOS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.159"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            495..959
                     /locus_tag="J8C37_003028"
     CDS             495..959
                     /locus_tag="J8C37_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAZ5306384.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            943..1572
                     /locus_tag="J8C37_003029"
     CDS             943..1572
                     /locus_tag="J8C37_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAZ5306385.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1926..3404
                     /gene="msr"
                     /locus_tag="J8C37_003030"
     CDS             1926..3404
                     /gene="msr"
                     /locus_tag="J8C37_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAZ5306386.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3451..3897
                     /locus_tag="J8C37_003031"
     CDS             3451..3897
                     /locus_tag="J8C37_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAZ5306387.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4096..4611)
                     /locus_tag="J8C37_003032"
     CDS             complement(4096..4611)
                     /locus_tag="J8C37_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAZ5306388.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4728..>5480
                     /locus_tag="J8C37_003033"
     CDS             4728..>5480
                     /locus_tag="J8C37_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAZ5306389.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELE"
BASE COUNT         1738 a          910 c         1146 g         1686 t
ORIGIN      
        1 gttattaaaa agaggtagtg aaaaagctgc cctgcatcaa gtagtaagaa cagccaaaca
       61 aaaacagatt ttcatgtttt ttgtttggtt gaacttatta ccgcagatgc aagtttttga
      121 acaccgttta ctcaaaaaag gttgtgacaa catcttgtca taactttttt attactgttt
      181 aagcatactg atccaatttc taatattcga cgggattctt gcaaaaggcg atttcggaaa
      241 tttttggcgg tgtaaaatta tctactgcag aagagctatc ttctatagtt tgataagaat
      301 caataatctt ggttttagcg ttgtgctttc tttatcattt tttccgttca ttctacaaaa
      361 aatttctttt ctctatactt ttctattgag agcgtttttt ttattacaca gctgattctt
      421 tgactttttt ctttttttat tctattattg acaagtcaat cgttgacgag tcaattatta
      481 gaaaggagga aattatgtct ttagaagaga ttagtcacct tttatatcaa atcaaattag
      541 ccgatcaaaa aatcgtccag atctttgaag aaaatatcgg tataagtctt actagatacg
      601 agatcatgat gagactgaaa gaagaaaatc cattgctaca aagcagtttg caagaaaagt
      661 tgcagattga tcaaggagca gttactcgtc atctgaaggt attggaagca aatggctatg
      721 tttcgcgtca gagaaatcct cagaacaacc gggaagtgta tgtagcgcta acagaaaaag
      781 cccagaagaa aatcaagcat tgtgaaatgg accataaaga tacaggttcc gtgctatccc
      841 cggctttcag cgatgtagaa atgaaacaat ttctaacact tttagataaa ttgaataccc
      901 aactttgcaa tataacaaca gaaacgaaag aggttaataa taatggaaca aaaaaatgat
      961 ttttcaacaa ttatgactgg tagaagatcc gttcgcgtat atgatgaaac atacaaaatc
     1021 ccacacgaag aaatgctgga aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat
     1081 atgaaaccat ggcgctttgt ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct
     1141 ttgatccgtt tcaatacgag acaaaatgat acttcttctg caatggtcat gatttttggc
     1201 gatatgcaat gttacgaata tggcgaagaa atctacaacc aagcttacga atcagggaag
     1261 atgccaaaag aagtcagaga tcaacaatta gctgcgatca ttccttatta caagagtttt
     1321 tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc
     1381 atgttagttg cgcgtgatca tggctatgaa acaaatccaa ttggtggatt cgaagcagat
     1441 cagttggcag aggcatttgg tttggataaa gaccgttatg ttccagttat tattttatca
     1501 ataggaaaag cggtagaaga aggttacgag tctgttcgtt tagcaccaga aaagatcact
     1561 atgttcgaat aaaaggtttt cagttactat tcagtagcta ttgctttttt tgaatcgtgt
     1621 gtggtaacct aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg
     1681 aacttttgaa taacaactaa ataaattcaa aagacatgta taaacaacaa tttcatggat
     1741 ggagtatgtc cattttttcg aacgacagta gaataggtga aagctatgac agatttggtg
     1801 atttagtcat agttatttcg ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac
     1861 agatttttct gctctttttt agtatcgata attgttgttt tacatgaaat aggagtgtaa
     1921 aacatatgga aaatttagca gtaaatataa caaatctaca agtcagcttt ggcaaccagc
     1981 tagaactatc tattgattct cttcgtgtct atcagcaaga tcgtataggg atcataggag
     2041 aaaatggtgt cggtaaatcg actttgctca aactaatagc cggtgaactt tttcccgatc
     2101 atgggaaaat ccaaacagag atcaccttca actacctgcc tcaattaacc tatctcgctg
     2161 aagcaaagga cctaaatttg gaattagcca gtcatttcca gttaagactg gaagaaactt
     2221 cagagcggaa atggagcgga ggggaagaac gaaagatcga gttgatacgt cttctttctt
     2281 cttatgaaca agggatgctt ctagacgagc caacaaccca tctagataga aaaagtattg
     2341 atcgactgat tgaagagctt cgttattatt atggtacgct ggtttttgtt agtcatgatc
     2401 gctattttct agatgagcta gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag
     2461 agttttcggg gaattatagt gcctatctca ctcaaaagga attggagaaa aagactcagc
     2521 tacgagaagc agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa
     2581 agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca
     2641 gactggatcg gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga
     2701 atgcgaaaac attagagaga agactccaaa aaattggaga aacaaccaag ccgcagcaga
     2761 tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact ccacagccgc tatcccatca
     2821 tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt actggtaaat ggtgattttc
     2881 agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt aagacaacct
     2941 tattagaaca tatccgcaaa caaggagaag gaatccttct ctctccgaaa gtaagctttc
     3001 aagtatatca gcaaaagggt tatcaaatga catctgaaga atccatcatt cgttttgtca
     3061 tgagacaaac agagttttca gaatcgcttg tccgtagttt gctgaatcac ttagggtttg
     3121 ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg gggagaagcg acccgtctga
     3181 cgattgcttt gctttttact aagccaagta atgtgttgct gttagatgaa ccgactaatt
     3241 ttattgatat ggcaacgatc gaagctttag agaagctaat gcaaatatat ccgggaacga
     3301 ttttgtttac ttctcatgat tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa
     3361 taaaagggca gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc tacgatgatt
     3421 ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa ataaaaaaga aggaataatt
     3481 tttacaacaa tgatgtgttt tttgatggtc ttggggatga gcgcgtataa tttatggctg
     3541 catcaagatc ttcatttatc aagtttgttg attggtctag tgcctggatt cgtggtggct
     3601 ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt taaactacca
     3661 gtaaatccag aaagcaaggt ccaaatgatc ttaactattt catgcttgat ggttttagga
     3721 atggtgacat gtatgtcttt attcggtgtg atcatggaac aaggaatacc tgatcaatta
     3781 ttctctgctt atatgaatgc ttggaaaatg aatgtgattg ccgctttacc gttgcagttg
     3841 atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg
     3901 aacactgtta tttgaaaaaa ggccttggca tgctttttgt catagtctct tgaatgcagt
     3961 tctagaaaaa tagtgatccg aacgataggt tttggcctgt cgttcggatc gctttttctt
     4021 ttccaactgt attgctcttt tattgaagat aatttgctct tcaatagata tttctagaaa
     4081 ggttctgttt atcccttact caaaaaactg ctgcatttgt tctcttactt gctgacacca
     4141 ttggagttcc tgctgacaaa gcataattct gcgctggatc acaacataac gtccaaattc
     4201 ttgtttcttg tctagcgaga ggtcattcaa ttttttttga tttttttgca tgattttgtt
     4261 cagctgttgt tctctagtga atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa
     4321 agcatagatt tttgctagaa attcatcttt ttgcgtagga gcgggagttt cctctttgat
     4381 ccattcctct acaagagcac gtccttcatc agtcaagtgg tagatctttt tttgagagtg
     4441 ctcgtctttg tccaaaacgg tcacataacc ctgctggtct aacttggcaa gcgttgtata
     4501 gatctgactg tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt catacccaga
     4561 acgtggtttg gataaaagca cgcataataa tatataggct aaagtattca taaaaatcct
     4621 acctctcgtc ttattttctt tattatatca cagtaaagtt tccacgtttt acatgtgcat
     4681 attgacatgt aaaataaatc atgtatgatg ctaatagaga gaaagggatg gaaatattgg
     4741 agaatcaaat agatatacaa gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc
     4801 tagttgtagc agctacctgt atgatcgctt ttatgggagt gggtctagta gacccaatct
     4861 taaaaacgat tgctgcacag ttaaacgcta cgccagctga aacgaccctg ttatttacta
     4921 gttacatgtt agtcactggt gtggtgatgc tatttacagg tttcttatct agtcggatcg
     4981 gtttgaaaaa aacattgatg acaggattgt ttatcatcgt tgtattcgct ggtcttggcg
     5041 gattatctgg gaatatcgga atgctgattg ggttacgtgc cggctggggt gtcgggaatg
     5101 ctttatttgt ctctactgct ttagcaacga tcgtcagtat tttagttggt aacacagaaa
     5161 aagcaatcat gatgtatgaa gcagcacttg gctgcggaat ggctgttggc cctctagtcg
     5221 gtggggtctt gggcagtggt tcatggcgtt atccattttt tggggtagca tttttgatgt
     5281 tttgcggttt tatagcccta gcggtacttt tacctgaaac tgagaaacca aagagaaaag
     5341 taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca gttggactaa
     5401 tcgcattgtt atataatttc ggattcttta ctttacttgc gtatgcgcca tttttattga
     5461 ttggctattc tgaattagaa
//
LOCUS       DADOOS010000357         4973 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.170, whole genome shotgun sequence.
ACCESSION   DADOOS010000357 DADOOS010000000
VERSION     DADOOS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.170"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..687
                     /locus_tag="J8C37_003034"
     CDS             <1..687
                     /locus_tag="J8C37_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAZ5306390.1"
                     /translation="MNRQTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQN
                     ERTTPKKETALSNVKKQQGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVK
                     YALHQGKENVTHNVTDFKQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRR
                     KENPSSPVQNNQQHVRKRQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLN
                     KRTDKRGQQT"
     gene            684..1706
                     /locus_tag="J8C37_003035"
     CDS             684..1706
                     /locus_tag="J8C37_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAZ5306391.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            1723..2652
                     /locus_tag="J8C37_003036"
     CDS             1723..2652
                     /locus_tag="J8C37_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAZ5306392.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            2897..3013
                     /locus_tag="J8C37_003037"
     CDS             2897..3013
                     /locus_tag="J8C37_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAZ5306393.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3029..4948
                     /gene="tet(M)"
                     /locus_tag="J8C37_003038"
     CDS             3029..4948
                     /gene="tet(M)"
                     /locus_tag="J8C37_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAZ5306394.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT         1758 a          837 c          994 g         1384 t
ORIGIN      
        1 atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca
       61 gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat
      121 actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag
      181 caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa
      241 aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat
      301 gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa
      361 caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa
      421 aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga
      481 aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa
      541 accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa
      601 aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga
      661 acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct
      721 tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca
      781 gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag
      841 tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg
      901 tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc
      961 aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac
     1021 aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag
     1081 attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg
     1141 ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa
     1201 aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg
     1261 gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat
     1321 ttgatgatga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct
     1381 atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg
     1441 tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc
     1501 aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata
     1561 atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg
     1621 caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg
     1681 gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag
     1741 cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt
     1801 ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt
     1861 ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt
     1921 gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa
     1981 tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat
     2041 gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt
     2101 aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg
     2161 aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac
     2221 aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg
     2281 gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa
     2341 cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta
     2401 gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt
     2461 aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc
     2521 tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa
     2581 acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag
     2641 attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt
     2701 tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact
     2761 atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata
     2821 gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt acccagttta
     2881 agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc
     2941 tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct
     3001 tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca
     3061 tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac
     3121 agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca
     3181 gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa
     3241 catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt
     3301 tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat
     3361 attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga
     3421 ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga
     3481 aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga
     3541 atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc
     3601 cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg
     3661 ttctctgttc cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat
     3721 agaagttatt actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg
     3781 aaatgttttc aaaattgaat atacaaaaaa aagacaacgt cttgcatata tacgccttta
     3841 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
     3901 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
     3961 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
     4021 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
     4081 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
     4141 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
     4201 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
     4261 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
     4321 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
     4381 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
     4441 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
     4501 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
     4561 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
     4621 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
     4681 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
     4741 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     4801 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     4861 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     4921 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa ata
//
LOCUS       DADOOS010000358         4460 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.179, whole genome shotgun sequence.
ACCESSION   DADOOS010000358 DADOOS010000000
VERSION     DADOOS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.179"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            199..981
                     /locus_tag="J8C37_003039"
     CDS             199..981
                     /locus_tag="J8C37_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAZ5306395.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1107..1838)
                     /gene="erm(A)"
                     /locus_tag="J8C37_003040"
     CDS             complement(1107..1838)
                     /gene="erm(A)"
                     /locus_tag="J8C37_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAZ5306396.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1896..1983)
                     /locus_tag="J8C37_003041"
                     /pseudo
     CDS             complement(1896..1983)
                     /locus_tag="J8C37_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2347..3009
                     /locus_tag="J8C37_003042"
     CDS             2347..3009
                     /locus_tag="J8C37_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAZ5306397.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3244..3387
                     /locus_tag="J8C37_003043"
                     /pseudo
     CDS             3244..3387
                     /locus_tag="J8C37_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            3537..3737
                     /locus_tag="J8C37_003044"
     CDS             3537..3737
                     /locus_tag="J8C37_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAZ5306398.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(3991..>4460)
                     /locus_tag="J8C37_003045"
     CDS             complement(3991..>4460)
                     /locus_tag="J8C37_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAZ5306399.1"
                     /translation="QDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGI
                     GDIGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQ
                     RILFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         1482 a          696 c          833 g         1449 t
ORIGIN      
        1 aaccaaattc aaaaattata tggagatctg tataataaag aataattatt aatctgtaga
       61 caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca gtcatttagg
      121 tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata tgaacataat
      181 cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa tcaagcgatt
      241 caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt atatctattt
      301 ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct agtcgtcgtg
      361 aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat gactatatca
      421 ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat aaataggagt
      481 gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga gtggctcagg
      541 ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt ggctattgtt
      601 ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc aagtatactt
      661 gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc agaactaatt
      721 gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat gtggcaaaca
      781 gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat acctctttta
      841 cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg agagtgtgat
      901 gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct
      961 atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta cttttaaaac
     1021 tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa
     1081 attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt gaaaatagaa
     1141 agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt caaagcctgt
     1201 cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt ataaacaaaa
     1261 gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc aagaacaatc
     1321 aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt tttgagcatt
     1381 tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt ttgcaatctt
     1441 ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc aaaggtaatt
     1501 cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt atagtttata
     1561 tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt ctcagagggg
     1621 tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc agtaactgat
     1681 cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat ttctattacg
     1741 ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt tttagaagta
     1801 ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc cttactggtt
     1861 ataatgaact gacattaagc gttcattata accaattaat tttgatttgg ttgataacga
     1921 actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt ccattttttt
     1981 cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat aattgaaacc
     2041 tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac ctttatatgt
     2101 aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt ttgaaacttg
     2161 ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac agtgacttta
     2221 ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag ttttatcccc
     2281 aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta ggaatggggg
     2341 atctttttga atacaataga atcacagttt gataaggttg cagaagatta cgatttcgtg
     2401 aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa gaaaggcaga
     2461 gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag ttattatgat
     2521 gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc taaacgtcaa
     2581 ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa tgagaagttt
     2641 gattttattg taagccgaac aacctttcat catttggatg atatagccag cgtaatacag
     2701 caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa tgtatctgaa
     2761 gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt tttaccgcat
     2821 tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac atctaaatca
     2881 tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta cgatttatat
     2941 gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg ggttgtctgg
     3001 acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt tcagagctat
     3061 cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta tgttactatt
     3121 aagtaattta agatacaggt tttactattt ttgaaataag taccttccac ttcaaataca
     3181 tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc ttgataatat
     3241 gaaattccat cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg
     3301 atggggatca ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga
     3361 gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt
     3421 acaattatat tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg
     3481 tacacgagca atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg
     3541 aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag
     3601 atggaagcga cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag
     3661 aagaaggtca agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa
     3721 acgttgtaaa agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg
     3781 agagaacaaa gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt
     3841 atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca
     3901 aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca
     3961 ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg
     4021 caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat
     4081 cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg
     4141 gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc
     4201 acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac
     4261 agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat
     4321 atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg
     4381 cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc
     4441 aggaacacct acatcttgtg
//
LOCUS       DADOOS010000359         2279 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.233, whole genome shotgun sequence.
ACCESSION   DADOOS010000359 DADOOS010000000
VERSION     DADOOS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.233"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..293
                     /locus_tag="J8C37_003046"
     CDS             <1..293
                     /locus_tag="J8C37_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ5306400.1"
                     /translation="QADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRD
                     QELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            551..2053
                     /locus_tag="J8C37_003047"
     CDS             551..2053
                     /locus_tag="J8C37_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAZ5306401.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2120..>2279
                     /locus_tag="J8C37_003048"
     CDS             2120..>2279
                     /locus_tag="J8C37_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ5306402.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIH"
BASE COUNT          810 a          406 c          497 g          566 t
ORIGIN      
        1 aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg atccacggaa
       61 aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt
      121 gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt gtcttaagta
      181 ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca ttagaaaaaa
      241 caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag tagttgacaa
      301 aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
      361 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggaa
      421 gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct ttttgtgtgt
      481 atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag
      541 gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg acagccaagg
      601 cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac taggacttat
      661 cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca aactacctta
      721 tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga caaaagataa
      781 agaacgttta acctattacg tgttaaatga tattacggat tttgagatat gggaaatcga
      841 aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
      901 atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg acactcattt
      961 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac aagtagcggc
     1021 ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg gatttatcga
     1081 tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa
     1141 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga
     1201 aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc
     1261 tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata ctgaatctag
     1321 acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac gatccaaagc
     1381 aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa aagatatcga
     1441 atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac ttttaagcgt
     1501 agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt ttacaatcga
     1561 gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat ccattatcca
     1621 ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc caaaacatct
     1681 gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg cggaatttgc
     1741 agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc ttgggatgga
     1801 aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga aaaaagtgga
     1861 attggctaaa tctttatcac agccagctga actatataca tgggatgaac cattgaatta
     1921 tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga aacctgccat
     1981 gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga ttatttctct
     2041 tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg ggaactttgc
     2101 caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc
     2161 acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac agatgcaggc
     2221 acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccatt
//
LOCUS       DADOOS010000360         2279 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.234, whole genome shotgun sequence.
ACCESSION   DADOOS010000360 DADOOS010000000
VERSION     DADOOS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.234"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..293
                     /locus_tag="J8C37_003049"
     CDS             <1..293
                     /locus_tag="J8C37_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAZ5306403.1"
                     /translation="QADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRD
                     QELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            551..2053
                     /gene="lsa"
                     /locus_tag="J8C37_003050"
     CDS             551..2053
                     /gene="lsa"
                     /locus_tag="J8C37_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAZ5306404.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQDFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2120..>2279
                     /locus_tag="J8C37_003051"
     CDS             2120..>2279
                     /locus_tag="J8C37_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAZ5306405.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIH"
BASE COUNT          809 a          406 c          497 g          567 t
ORIGIN      
        1 aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg atccacggaa
       61 aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata atcgctattt
      121 gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt gtcttaagta
      181 ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca ttagaaaaaa
      241 caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag tagttgacaa
      301 aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg atccgtgcat
      361 tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg agacaaggaa
      421 gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct ttttgtgtgt
      481 atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag
      541 gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg acagccaagg
      601 cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac taggacttat
      661 cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca aactacctta
      721 tcaagggcaa gtaatccatc agcaagattt tgcctatttc ccgcaacaga caaaagataa
      781 agaacgttta acctattacg tgttaaatga tattacggat tttgagatat gggaaatcga
      841 aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat tcagcacact
      901 atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg acactcattt
      961 cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac aagtagcggc
     1021 ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg gatttatcga
     1081 tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt atcaagggaa
     1141 tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg ctcaaaatga
     1201 aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag ccgaatggtc
     1261 tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata ctgaatctag
     1321 acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac gatccaaagc
     1381 aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa aagatatcga
     1441 atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac ttttaagcgt
     1501 agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt ttacaatcga
     1561 gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat ccattatcca
     1621 ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc caaaacatct
     1681 gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg cggaatttgc
     1741 agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc ttgggatgga
     1801 aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga aaaaagtgga
     1861 attggctaaa tctttatcac agccagctga actatataca tgggatgaac cattgaatta
     1921 tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga aacctgccat
     1981 gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga ttatttctct
     2041 tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg ggaactttgc
     2101 caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa cttggaacgc
     2161 acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac agatgcaggc
     2221 acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa tcatccatt
//
LOCUS       DADOOS010000361         1349 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.286, whole genome shotgun sequence.
ACCESSION   DADOOS010000361 DADOOS010000000
VERSION     DADOOS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.286"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            46..297
                     /locus_tag="J8C37_003052"
                     /pseudo
     CDS             46..297
                     /locus_tag="J8C37_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="J8C37_003053"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="J8C37_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAZ5306406.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DADOOS010000362         1194 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.300, whole genome shotgun sequence.
ACCESSION   DADOOS010000362 DADOOS010000000
VERSION     DADOOS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.300"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            55..138
                     /locus_tag="J8C37_003054"
     CDS             55..138
                     /locus_tag="J8C37_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAZ5306407.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            263..1000
                     /gene="erm(B)"
                     /locus_tag="J8C37_003055"
     CDS             263..1000
                     /gene="erm(B)"
                     /locus_tag="J8C37_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAZ5306408.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          457 a          189 c          183 g          365 t
ORIGIN      
        1 aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg
       61 gtattccaaa tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt
      121 gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt
      181 tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg
      241 taatcacttc aggagtgata acatgaacaa aaatataaaa tattctcaaa actttttaac
      301 gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt
      361 ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca
      421 ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact
      481 gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca
      541 gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt
      601 ggtttttgaa agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg
      661 taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca
      721 attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat
      781 aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt
      841 tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca
      901 agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc
      961 tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta
     1021 aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt atagggcacc
     1081 tctaataact accaattaat tcacctcaaa atgaaattag aaaaaaacag cgaaattttt
     1141 aaatctattt cttatggcgg attttataat gaacttagcc atttaaaata aaaa
//
LOCUS       DADOOS010000363         1194 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.301, whole genome shotgun sequence.
ACCESSION   DADOOS010000363 DADOOS010000000
VERSION     DADOOS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.301"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="J8C37_003056"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="J8C37_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAZ5306409.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          427 a          172 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacacaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DADOOS010000364         1194 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.302, whole genome shotgun sequence.
ACCESSION   DADOOS010000364 DADOOS010000000
VERSION     DADOOS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.302"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="J8C37_003057"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="J8C37_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAZ5306410.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DADOOS010000365          861 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.328, whole genome shotgun sequence.
ACCESSION   DADOOS010000365 DADOOS010000000
VERSION     DADOOS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.328"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            146..694
                     /gene="aac(6')"
                     /locus_tag="J8C37_003058"
     CDS             146..694
                     /gene="aac(6')"
                     /locus_tag="J8C37_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAZ5306411.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          306 a          127 c          190 g          238 t
ORIGIN      
        1 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
       61 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
      121 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
      181 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
      241 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
      301 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
      361 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
      421 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
      481 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
      541 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
      601 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
      661 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
      721 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
      781 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
      841 cttagtttcg agtagtaaga a
//
LOCUS       DADOOS010000366          660 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.347, whole genome shotgun sequence.
ACCESSION   DADOOS010000366 DADOOS010000000
VERSION     DADOOS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.347"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>660
                     /locus_tag="J8C37_003059"
     CDS             <1..>660
                     /locus_tag="J8C37_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAZ5306412.1"
                     /translation="GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETN
                     VKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL
                     DYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCL
                     CHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRM
                     YG"
BASE COUNT          275 a           47 c          110 g          228 t
ORIGIN      
        1 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
       61 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      121 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      181 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      241 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      301 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      361 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      421 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
      481 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
      541 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
      601 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
//
LOCUS       DADOOS010000367          568 bp    DNA     linear   BCT 02-APR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00015557
            SAMN18082976-rid11849363.guided.354, whole genome shotgun sequence.
ACCESSION   DADOOS010000367 DADOOS010000000
VERSION     DADOOS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18082976
            Sequence Read Archive: SRR13825532
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 31-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000995186.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000995186.1
            Genome Coverage       :: 59x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/31/2021 12:16:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,060
            CDSs (total)                      :: 3,016
            Genes (coding)                    :: 2,942
            CDSs (with protein)               :: 2,942
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 28 of 74
            Pseudo Genes (incomplete)         :: 35 of 74
            Pseudo Genes (internal stop)      :: 20 of 74
            Pseudo Genes (multiple problems)  :: 8 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18082976-rid11849363.guided.354"
                     /strain="AUSMDU00015557"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /collection_date="2018"
     gene            <1..>568
                     /locus_tag="J8C37_003060"
     CDS             <1..>568
                     /locus_tag="J8C37_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAZ5306413.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLV"
BASE COUNT          226 a           53 c          104 g          185 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagtta
//
